LOCUS       c00023_NZ_BMBU..        7524 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_BMBU01000023.1 Bifidobacterium pullorum strain LMG 21816
            contig23, whole genome shotgun sequence.
ACCESSION   NZ_BMBU01000023
VERSION     NZ_BMBU01000023
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-13 23:37:26
            Original ID  :: NZ_BMBU01000023.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7524
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7524
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3670:7235]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3671..7235
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7524
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7524
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(165..1535)
                     /locus_tag="ctg23_1"
                     /transl_table=11
                     /translation="VKISVRNLEPTKVKLTITVDPEEFNPYLDAARKEIAKQVTVPGFR
                     KGHVPGKIIDQRIGFAAVAGEAVNNGVPELYSKALEEKKIRPMAQPEIDVQEVPSSAKD
                     ETKLKFVATVERRPDIELPELEGMEIDVPKAEITDEDVNNRLENLRQRFGTLVGVDRPA
                     AKGDFANIDLVAQIDGETVDSQEGVSYELGSNTMLDGLDEALDGLSAGEETTFEGTLEA
                     GEHEGEKAQIKVKVNSVKAEELPELDDDFAQEASEFDTLDELKADIRKACERDAEGRQA
                     TEARDAFIAKLKEGLEIPVPKGVKAEAAEQHLKGMTPDPEQATKEQKAEAEEAAENELR
                     DQMVLDALAEKMDVKVSQMDVTNFLASIAQQYGMDPSAFINAIIRNGQLGSAVEEVGRS
                     KGLLAGMRAVTFKCEGEVLDLSSFLGDVAEDEEAESVEAASAAAAVADELAQAEDAE"
     CDS             complement(1589..2890)
                     /locus_tag="ctg23_2"
                     /transl_table=11
                     /translation="MAKGRRLNREPRLQTEPRDGVPDVIDTPEGYRRYCARLRETSGPL
                     AADAERASGFRYGHEDWLVQFKRDGAGIGLLDPVALDAAGVRWNEFNDAVGDAMWIIHD
                     ASQDLPGFAQIGLEPRALFDTEIAARLLGLHRFGLAAVMEQYLGITLAKEHSAADWSYR
                     PLPRDWRNYAALDVELLIELEQRLSHDLKRQGKDEWARQEFAHTLHEGMLPRPGHPVPW
                     MRISRITSLSRDRQGLAVAKALWQERDRLAQHYDIAPGLLLSDAAIIEAAQKKPRNARE
                     FRAIRSLNERVRIHTGGEQDKMFERYAPIQRAVKPNVWKTVIQEALALRPDQWPSVPAQ
                     PADAPNNAPRSMRIWSTRHPERYRRLQAVRQTINQIAEDTRTPAEMIIKPQIIRNLCWM
                     DDPAGGDVAEFLTEQGARPWQVSLVAASVSRVIM"
     CDS             complement(2865..3494)
                     /locus_tag="ctg23_3"
                     /transl_table=11
                     /translation="MASIYSFPMNTPEEERTGTAHTEDDIRPSRPAGVPDEVWHAVESV
                     RVMRRLDGVDYREIPVPSASADYGIGVELECAERDGTGVRVASGWIMILYAGVARSGWW
                     SRWRCVAFASLPLEEGGDCLTSSMYWEDMADVLSGVDPSHLKGTVTVTQNTSFGGMEAE
                     PHTGCEMRVSWTPAEPPEGVDAGAQVMAWARFLRASLRNGERETIE"
     CDS             complement(3671..4552)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.1;
                     E-value: 1.5e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_4"
                     /sec_met_domain="Radical_SAM (E-value: 5.3e-31, bitscore:
                     96.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSETTEFRTTTRHMLKRSKQYASQTLMGGLSGFESPIGLDRRDRI
                     EALRTGDIGFVHSWDINTSVDGPGTRMTVFMSGCPLRCQYCQNPDTWKMRDGQPVYLEA
                     MIKKVERYADLFKATGGGITFSGGESMMQPAFVSRVFRAAKEMGVHTCLDTSGFLNRNY
                     TDEMIEDIDLCLLDVKSGDEETYHKVTGGLLAPTIEFGQRLAKAGKKIWVRFVLVPGLT
                     DSEENVENVARICETFGEAIEHIDVLGFHQLGRPKWHELRIPYPLEDAKGPSAKLKARV
                     KEQFEDHGFVVY"
     CDS             complement(4860..7235)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_5"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-38, bitscore:
                     119.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6e-238, bitscore:
                     779.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVDATAVSPEELNAKAWDGFEGGHWQEDIDVRDFIQKNYTPYE
                     GDESFLADATEKTKHLWQYLDDNYLSVERKQRVYDVDTHTPADVDAFPAGYIDSPEVDN
                     VIVGLQTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPQIKKIFTKYRKTHNDGVFGVYT
                     KQIKVARHNKILTGLPDAYGRGRIIGDYRRVALYGVNALIKFKQRDKDSIPYRNDFTEP
                     EIEHWIRFREEHDEQIKALKKLINLGNEYGLDLSRPAQTAQEAVQWTYMGYLASVKSQD
                     GAAMSFGRNSTFFDIYIERDLKNGTLDEAGAQELIDNIVMKLRIVRFLRTKDYDAIFSG
                     DPYWATWSDAGFGDDGRTMVTKTSFRLLNTLTLEHLGPGPEPNITIFWDPKLPEGYKRF
                     CAKISIDTSAIQYESDKEIRGHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAI
                     NGGRDEMTGMQVIDKGVIDPIQPEADGTLDYEKVKNNYEKALEWLSETYVMALNIIHYM
                     HDKYAYESIEMALHDKEVYRTLGCGMSGLSIAADSLSAVKYAKVYPIYNKDAKDMPGHE
                     YEYVEGADDDLVVGYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQIKRLPVYRGAVPTQ
                     SILTITSNVEYGKNTGSFPSGHEKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDAL
                     DGISLTNTITPDGLGRDEDERITNLVGILDAGNGHGLYHANINVLRKEQLEDAVEHPEK
                     YPHLTVRVSGYAVNFVKLTKEQQLDVISRTFHQGAVVD"
ORIGIN
        1 cgcacccccc ccccacccac ccccgccgcc aacagcccca ccccccgccg acgtccaccc
       61 accccacacc ccccccgcac caccttttgg gggctgcacc cggcccaccg gttaccagcc
      121 cacaatcatc gttattcaga cccttgccac ggcaaggtca caactcactc ggcgtcctcg
      181 gcctgggcga gctcgtcggc cacggcggca gcggcagagg cggcctcaac ggactcggcc
      241 tcctcatcct cagccacgtc accgaggaag gagctcagat cgagcacctc gccctcgcac
      301 ttgaaggtca cggcacgcat accggccagc aggcccttcg agcggccgac ctcctccacg
      361 gcggagccca gctggccgtt gcggatgatc gcgttgatga acgccgacgg atccatgccg
      421 tactgctggg cgatggaggc caggaagttg gtcacatcca tctgggagac cttgacgtcc
      481 atcttctcgg ccagggcatc gagcaccatc tggtcgcgca gctcgttctc ggcggcctcc
      541 tcggcctcgg ccttctgctc cttggtggcc tgctccggat ccggggtcat gcccttcagg
      601 tgctgctcgg cggcctcggc cttgacgccc ttcggcaccg ggatctccag gccttccttg
      661 agcttggcga tgaacgcgtc acgggcctcg gtggcctggc ggccttcggc gtcacgctcg
      721 caggccttgc ggatatcggc cttcagctcg tcgagggtgt cgaactcgga ggcctcctgg
      781 gcgaagtcgt cgtccagctc cggcagctcc tcggccttga ccgagttgac cttgaccttg
      841 atctgggcct tctcgccctc atgctcgccg gcttccagcg tgccttcgaa ggtggtctcc
      901 tcgccggcgg acaggccgtc cagggcctca tccagaccat caagcatggt gttggagccg
      961 agctcgtagc tcacgccctc ctgggagtcg acggtctcgc cgtcgatctg cgcgaccagg
     1021 tcgatgttcg cgaagtcgcc cttggcggcc ggacggtcga cgcccaccag ggtgccgaaa
     1081 cgctggcgca ggttctccag acggttgttc acgtcctcat cggtgatctc ggccttcggc
     1141 acgtcgatct ccatgccttc gagctccggc agctcgatgt ccggacgacg ctccacggtg
     1201 gcgacgaact tgagcttggt ctcgtccttg gcggaggacg gcacttcctg cacgtcgatc
     1261 tccggctggg ccatcggacg gatcttcttc tcctcgagcg ccttggaata cagctccggc
     1321 acaccgttgt tcacggcctc gccggcaacg gcggcgaaac cgatgcgctg gtcgatgatc
     1381 ttgcccggga catggccctt acggaagccg ggcacggtga cctgcttggc gatctccttg
     1441 cgggcggcat ccagatacgg gttgaactcc tcgggatcga cggtgatggt gagcttcacc
     1501 ttggtgggct cgaggttcct gacgctgatt ttcacgcttg cgctcctgtg aaatattcgt
     1561 attcgttatt cctgccgtta ggcaaccgtt acatgataac gcgactcacg gacgctgcga
     1621 ccaacgacac ctgccatggc cgggcaccct gttcggtgag gaattccgcg acatccccgc
     1681 cggcgggatc gtccatccag cacaggttgc gaatgatctg cggcttgatg atcatctccg
     1741 ccggggtccg cgtatcctcg gcgatctgat tgatcgtctg ccggaccgcc tgcagccggc
     1801 gataccgttc gggatgccgg gtggaccata tccgcatcga acgcggcgcg ttgttcgggg
     1861 cgtcggcggg ctgcgcgggc acgctgggcc actggtcggg ccgcagcgcc agcgcctcct
     1921 ggatcacggt cttccagaca ttcggtttga cggcgcgttg aatcggcgca taccgctcga
     1981 acatcttgtc ctgttcgccg ccggtatgga tccgcacccg ctcgttcaac gaacggatgg
     2041 ccctgaattc cctggcgttg cggggtttct tctgcgccgc ctcgatgatg gccgcatcgg
     2101 atagcagcag tcccggggct atatcgtagt gttgcgccag ccggtcgcgc tcctgccaga
     2161 gcgccttggc cacggccagt ccctgccggt cacggctcaa cgaggtgatc cgggagatcc
     2221 gcatccaagg caccggatgc cccggacggg gcagcatgcc ctcatgcagc gtgtgtgcga
     2281 actcctggcg cgcccactcg tccttgccct gccgtttgag atcgtgcgac aggcgctgct
     2341 caagctcgat gagcagttcc acgtccagcg ccgcgtagtt gcgccagtcc cgaggcagcg
     2401 gacggtagga ccagtcggcc gccgaatgct ccttggccag ggtgatgccc agatactgct
     2461 ccatcaccgc cgccagaccg aaccgatgca atcccaacag ccgggcggcg atctccgtgt
     2521 cgaacaaggc ccgaggctca agaccgatct gggcgaaccc cggcagatcc tgcgaggcgt
     2581 catggatgat ccacatcgcg tcccccacgg cgtcgttgaa ctcgttccaa cgcacgcccg
     2641 cggcgtccaa cgccaccggg tccagcagac cgatgcccgc accgtcacgt ttgaactgga
     2701 ccagccaatc ctcgtgcccg tatcggaaac cggaggcgcg ttcggcatcg gccgccaacg
     2761 gccccgaggt ctcgcgcaac ctggcgcagt accggcgata cccttcaggt gtgtcgatga
     2821 cgtcggggac gccatcccgc ggctcggtct gcagcctcgg ctccctattc aatcgtctcc
     2881 ctttcgccat tccgcagcga tgcgcgcagg aatcgagccc acgccatcac ctgtgccccg
     2941 gcgtcgacgc cttccggcgg ttccgccggc gtccatgata cgcgcatctc gcacccggtg
     3001 tgcggttcgg cctccatacc gccgaaggac gtgttctgcg tcaccgtcac cgtgcccttc
     3061 agatggctgg gatcgacgcc gctcagcaca tcggccatat cctcccagta catcgacgac
     3121 gtcaggcagt cgccgccctc ctccaacggc agactggcga acgccacgca acgccaccgc
     3181 gaccaccagc ccgaccgcgc gacgcccgca tacaggatca tgatccaccc ggaagccaca
     3241 cggacaccgg tcccgtcgcg ctccgcgcat tccaattcca cgccgatgcc gtagtcggcc
     3301 gatgcggacg gcaccgggat ctcccgatag tccacgccgt cgaggcggcg catcacacgc
     3361 accgactcga cggcatgcca gacctcgtcg ggcacgccgg cgggacgcga cggccggatg
     3421 tcgtcctcag tatgcgcggt tcccgtccgc tcttcctcgg gcgtgttcat cgggaacgag
     3481 taaatgctcg ccatattcca aggctacgtg tctcggcccc gattcgtggt atcacacgcg
     3541 cggggacgtc tcagtagaca acaaacccgt gacgttcggt ccagtggacc gaacgtagcc
     3601 gggcacccca ccacgggggt gcccgcgcac aaacaacggc agttcgagga ccccccacac
     3661 acacaaaacg tcagtagacg acgaaaccgt ggtcctcgaa ctgctccttg acccgcgcct
     3721 tgagcttggc cgaggggccc ttggcgtcct ccagcgggta cgggatgcgc agttcatgcc
     3781 acttgggacg gccgagctgg tggaaaccaa gcacgtcgat atgctcgatg gcctcgccga
     3841 aggtctcgca gatcctggcc acgttctcca cgttctcctc ggaatcggtc agaccgggca
     3901 ccagcacgaa gcgcacccag atcttcttgc cggccttggc caggcgctgc ccgaactcga
     3961 tggtcggcgc gagcaggcca ccggtgacct tgtggtaagt ctcctcgtca ccggatttga
     4021 cgtcgagcag gcacaggtcg atgtcctcga tcatctcgtc ggtgtagttg cggttgagga
     4081 atccggaggt gtccaggcag gtgtgcacgc ccatctcctt ggccgcgcgg aacacacggg
     4141 agacgaacgc cggctgcatc atcgactcgc cgccggagaa cgtgatgccg ccgccggtgg
     4201 ccttgaacag gtcggcgtat cgctcgacct tcttgatcat ggcctccagg tacaccggct
     4261 ggccgtcgcg catcttccag gtgtcggggt tctggcagta ctggcagcgc agcgggcagc
     4321 cgctcatgaa cacggtcatg cgggtgccgg ggccgtccac ggaggtgttg atgtcccacg
     4381 agtgcacgaa gccgatgtcg cctgtgcgca gggcctcgat gcggtcgcgc cggtccaggc
     4441 cgatcgggga ttcgaatccg gacaggccgc ccatcagggt ctggctggcg tattgtttcg
     4501 accgcttcag catatggcgg gtcgtggtgc ggaattcggt ggtctcagac atcctcgtcc
     4561 tccttatcgc gcgaacgcgc gcacatacac atgatactga ttgccatcgt aaggccttgc
     4621 atcgccggac acgcggcttt ccgctgtgag gtgcctcacg tgtcccgagc ggttctcccc
     4681 caccgtcgca tgcgggcctt tcgaccgtgc tgcggctgcc tccttgtcga agggaggaaa
     4741 ctacgacccg cgccgtgcgc ggagcctctc ccgtcaaagg gagggaatag gaaaggcggg
     4801 gccttccgaa gaagaccccg ccggaccgtt cagccgttca cacggtgcgc gtcatcgact
     4861 cagtcgacga cggcgccctg gtggaacgta cgggagatca cgtcgagctg ctgctccttg
     4921 gtgagcttga cgaagttcac cgcgtagccg gagacgcgca cggtcaggtg cgggtacttc
     4981 tccgggtgct cgacggcatc ctcgagctgc tccttgcgca gcacgttgat gttggcatgg
     5041 tacaggccgt ggccgttgcc cgcatccagg atgccgacca ggttggtgat gcgctcatcc
     5101 tcgtcgcggc ccaggccgtc gggggtgatc gtgttggtca gcgagatgcc gtccagcgcg
     5161 tcgttgtagt cgatcttgcc gaccgagaac atcgacggca gcatgccgtg ggagtccatg
     5221 ccgttctccg ggttcgcgcc cggggcgtac ggggtgccct tctcgtggcc ggacgggaag
     5281 gagccggtgt tcttgccgta ctcgacgttc gaggtgatcg tcaggatgga ctgggtcggg
     5341 acggcgccgc ggtagaccgg caggcgcttg atctggccca tgaccgtgga gacgacccac
     5401 ttggcgatgt cgtcggcgcg atcgtcgtcg ttgccgtaga ccgggaactc gccctcggtg
     5461 cggtagccga ccaccagatc gtcatcggcg ccctcgacat actcgtactc gtggcccggc
     5521 atgtccttgg cgtccttgtt gtagatcggg tagaccttgg cgtacttgac ggcggacagc
     5581 gagtcggcgg cgatggacag accggacatg ccgcaaccca gggtgcggta cacctcctta
     5641 tcgtgcaggg ccatctcgat ggactcgtag gcgtacttgt catgcatgta atggatgatg
     5701 ttcagcgcca tcacgtaggt ctcggacagc cactcgaggg ccttctcgta gttgttcttg
     5761 accttctcgt aatccagggt accgtcggcc tcgggctgga tcggatcgat cacgcccttg
     5821 tcgatgacct gcatgccggt catctcgtca cggccgccgt tgatcgcgta cagcagcgcc
     5881 ttggcggagt tcacgcgggc ggcgaagaac tgcatctgct tgccgacgcg catcggggac
     5941 acgcagcagg cgatggccgc gtcgtcgccc cagtggccgc ggatctcctt gtcggactca
     6001 tactggatgg ccgaggtgtc gatggagatc ttggcgcaga agcgcttgta gccctccggc
     6061 agcttcggat cccagaagat cgtgatgttc ggctccgggc cgggtcccag gtgctcaagg
     6121 gtcagggtgt tgagcagacg gaacgaggtc ttggtgacca tggtgcggcc gtcgtcgccg
     6181 aagccggcgt ccgaccaggt cgcccagtac gggtcgccgg agaagatcgc gtcgtagtcc
     6241 ttggtgcgca ggaagcgcac gatgcgcagc ttcatgacga tgttgtcgat gagctcctgc
     6301 gcgccggcct cgtcgagcgt gccgttcttc aggtcgcgct cgatgtagat gtcgaagaag
     6361 gtcgagttgc ggccgaagga catggcggcg ccgtcctggc tcttgaccga ggccaggtag
     6421 cccatgtagg tccactgcac ggcctcctgg gcggtctggg ccggacggga caggtccagg
     6481 ccgtactcgt tgccgaggtt gatcagcttc ttcagggcct tgatctgctc gtcatgctcc
     6541 tcgcggaagc ggatccagtg ctcgatctcc ggctcggtga agtcgttgcg gtacgggatc
     6601 gagtccttgt cgcgctgctt gaacttgatc agggcgttca cgccgtacag ggccacacgg
     6661 cggtagtcgc cgatgatgcg gccgcgtccg taggcgtccg gcagaccggt gaggatcttg
     6721 ttgtggcggg cgaccttgat ctgcttggtg tacacgccga agacgccgtc gttgtgggtc
     6781 ttgcggtact tggtgaagat cttcttgatc tgcggatccg gctccttgcc ggcctccttg
     6841 atggcctgct cgaccatgcg ccagccgccg ttcggcatca tcgcgcgctt gcaggggacg
     6901 tcggtctgca gaccgacgat cacgttgtcg acctccgggc tgtcgatgta gccggccggg
     6961 aaggcgtcga catcggccgg ggtgtgggta tccacgtcgt agacgcgctg cttgcgctcc
     7021 acggacaggt agttgtcatc gagatactgc cacagatgct tggtcttttc ggtcgcatcg
     7081 gcgaggaagg actcatcgcc ctcgtacggc gtgtagttct tctgaatgaa gtcacgcacg
     7141 tcgatatctt cctgccaatg accgccttcg aagccgtccc acgccttggc gttgagctcc
     7201 tccggggaga ccgcggttgc atcaactgct gtcatatcca ctccttttgg gtgatcgtcg
     7261 gcgacgcatc gttacgccgt cgttggttac ctattgtagc cgcaggcagc tgaaaacaag
     7321 ctggagggca acgtgagctt cgtcacattt cgttatcgct cacatcgtgc ccggcggata
     7381 acacgacggc ggcccccccc ccgggggggg ggccccccac ccggaaaatt gtggccgggg
     7441 ggggggggaa ttaaggggac cccggggggc ccccccgcgg ggggggcccc ccccacgccc
     7501 cccccggggc caaaggggcc cccg
//
