LOCUS       c00048_NZ_FOBS..       14749 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_FOBS01000007.1 Syntrophus gentianae strain DSM 8423, whole genome
            shotgun sequence.
ACCESSION   NZ_FOBS01000007
VERSION     NZ_FOBS01000007
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 00:58:38
            Original ID  :: NZ_FOBS01000007.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14749
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14749
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[441:4749]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      442..4749
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..14749
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14749
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(442..1296)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_1"
                     /sec_met_domain="BcrAD_BadFG (E-value: 2.7e-64, bitscore:
                     210.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEYFAGIDIGSTMTKAVILGEGIAASVIGPTGAEQRRLANKVME
                     EALDQAGLAFEAISYVVATGYGRINVPFADRQITEITCHARGIHFLFPEARTVIDVGGQ
                     DSKAIRIDSQGRPHDFIMNDKCAAGSGRFIEIIADTLNIPLEAVGDLSLQSTSPATISN
                     LCTIWAQQEVASSLAQGIPVPDLLAGVHRSLADRISRMAHRLRLEKPVVLTGGGSKNKG
                     LIAALREYLNGDLLIPENPLITGALGAALMGRDLVEKARQNNLPLETRPRILEAIHLLG
                     SSD"
     CDS             complement(1311..2714)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_2"
                     /sec_met_domain="HGD-D (E-value: 2.3e-46, bitscore: 152.2,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEEKKEKKPQKRMTATHAAASIGPMVKEFIAGTNRARKEGTHKL
                     AYTFICCHHEEILRAMDVLPVWTENFAGVCGAKRDAERFMQRAESLGLSRSLCTYALCG
                     IGYDQWREELGETPPDAPWGGQARPDFMIQTGQILCDPRAKWYQASQQFMPDIPIYNID
                     LPYPLFQRDRDHREILGYYHRYIVKELRGLIAFVEKMTGKKMDYDRLSERVDLSDRIWN
                     LVHETYELRKAKPSPMGTGDAMNTMVPLNFMMATQESYNFFSDLKKELKEKIAKGEGEV
                     EEEKYRLMWGGGLPAWYALNDFNYFNSKGATFPVETTYRLVMPLDEMDLPKTSDPVERL
                     AWRWLGYWTFWYDKARKRPGSEPDVERLINWVEEYDIDGIVVHQAFSCRTWHVGLIWQL
                     KQLAKIYKPIPILMMGSGGQKEKTNREVPSLILESDIIDITSYSEIDTRNKIDAFIETL
                     ESVRTNRAA"
     CDS             complement(2745..3905)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_3"
                     /sec_met_domain="HGD-D (E-value: 2.5e-55, bitscore: 181.6,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTKNNIMAEVERQYENYGIRAKELCAGGKKAIGYICSFVPLEF
                     ITAAGCIPFRIRGDIHEPITKGDSLLETIVCPFIRSCFDLCVKDRYDFLSGIVIPHGCD
                     SMVRSYSVWSYSLNFPYFHFVNTPSVVKESSHEYFKEELQSYRKSLEDFTGKPISDADL
                     QEAIRLHNENRTKTRALYEFKKADPPLISAAELQKVLAVGASLPVEEANSLFDEVLAEL
                     GRRTEAPVKKGPRILLDGPCVDHLAISGIVEENGGNVVIDTTCMGTRDLWPLADEEGDP
                     VASLARRYLDKLNCPKTYRENTAGTFAGDAEDRFGDIGARAKEFAANGAILYLYKYCDP
                     FGFEVPARKAYYESLNIPLLCLEDTYSAGTIGQLKTRIQAFLEMIG"
     CDS             complement(3961..4749)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MreB_Mbl"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg48_4"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.5e-48, bitscore:
                     158.7, seeds: 18, tool: rule-based-clusters)"
                     /sec_met_domain="MreB_Mbl (E-value: 1.3e-07, bitscore:
                     23.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFFLGIDIGSFSAKGVLLEDQSVQASFACPSGGDYRGAAEQVRD
                     TLLSRAGIAPSGLSFVMATGYGAKQVLFADEEKPDITCQGRGVAFLLPSARTVLDCGDL
                     YSKVLRMDGEGHVHNFLLSGKCAGGSGRLLLIMAKVLRVKLEEIGPLSLKSRSKVDFST
                     GCVVFSESEAVSLIAEGVAKEDLLAGVHRALAAQLASLAERVGIEPDVVLTGGGAVDAG
                     LVQALEDSLRIPVRVPSNPLFTAALGAALLAREKSLSQGI"
     CDS             5239..6030
                     /locus_tag="ctg48_5"
                     /transl_table=11
                     /translation="MTEEGLEKKIRAVADMICEAGKVVVFTGAGVSTESGIPDFRSPGG
                     LWDRFDPDDFTIQKFLRSAQTRRKQWRILIEGGAFAEAQPNRAHLAVAELEKLGKLRCV
                     ITQNIDNLHQKAGNAPERVYELHGNMRWLKCLSCGDRISVPDMLQRTALRELDGFPFCE
                     RCQGLLKPDVIFFGEALPEDTLREATYEASNCDLMLVIGSSLVVYPAAYMPQYAKDVGA
                     KLVIINREETPYDAEADILLQGGAGEMMTRILAAVKDQIDL"
     CDS             complement(6049..6702)
                     /locus_tag="ctg48_6"
                     /transl_table=11
                     /translation="MAVNVLLADDHKIVRDGLRILIENHGNMNVVAEAENGQKAITLAK
                     ELHPQVIIMDISMPDMNGIDATRRITSDFPGIKVIALSMHTDRHFVVGMLEAGAAGYLL
                     KDCAFEELVSAIHTVLENHTYLSPTITDIVVRNYVHKEAKPSVSVSSELTARERELLQL
                     LAEGMTAKQIAKTLRVSVKTVETHRRNIAQKLGAGSVAELIKYAIREGLTTLDS"
     CDS             7082..7510
                     /locus_tag="ctg48_7"
                     /transl_table=11
                     /translation="MSKREYPDCPRVGVGAIVVREGRILLVKRAAEPNRGLWAIPGGSL
                     KLGETLKEGAEREVLEETGIVVEAKHPVYAFDLLERDQEGSFRFHFVIVDMLADYIRGD
                     LKAADDALDARWLLPEDLEQVDLSTSTVKILRHIKFYG"
     CDS             7609..8355
                     /locus_tag="ctg48_8"
                     /transl_table=11
                     /translation="MKVIYHDDFCQCYTTDPAAASGRMEAVVEAISGRVDFLKAEPAEE
                     SDISAVHTESHIALVEREGLYDIAALAAGGAIMAAEIGMNEPCFALIRPPGHHASAGSA
                     WGFCYFNNMAIALEKLKREGKIVKAFLLDFDLHFGDGTVNILERKEYVTIYNPSSCDRN
                     EYLRDIEKKLSSASFDIIAVSAGFDNHREDWGGLLTTEDYKTIGHMVWKICQKKRCGCF
                     AVLEGGYNHRVLGTNVLAFLEGIEGL"
     CDS             8416..8520
                     /locus_tag="ctg48_9"
                     /transl_table=11
                     /translation="MQAYRKYVEEPTTKPTQLAEERQLGVGSFPDAEG"
     CDS             complement(8497..9867)
                     /locus_tag="ctg48_10"
                     /transl_table=11
                     /translation="MLEDTIAAIATPAGVGGIGIVRISGPKSESILFRLFQSSHRISRY
                     QPRRLYHGDLIAPTTGQVLDEVLVSLMKAPSSFTGEDTLEIFCHGGILILEAVLSAVLE
                     AGARLAEPGEFTRRAFLHDRIDLTQAEAIGDVISARTSRGLETAVAHLKGKLKQKIDVL
                     RKMLIDILALLETEIEFSEDVESPAAETPAKLQSLSEHIEILLRTYDQGKIDRHGAAVV
                     IAGKPNTGKSSLLNCLLGEKRAIVTPIPGTTRDFIEEAINIQGLSIRLIDTAGIRPPED
                     RIEQEGIRMVWEKLAAADGVILLFDGSSSLTEEDRDLLKRLADYNVLPVINKTDLDHRL
                     GEEEITACFPGTSPLWISAKFGEGIPALKEKIRRLVMDRAGIQDGEIVISSLRQKMALE
                     KTLKFVAQARNSLEDGLSPEFAALDVREALEALGEIGGETATEEILDRIFSSFCIGK"
     CDS             complement(9909..10736)
                     /locus_tag="ctg48_11"
                     /transl_table=11
                     /translation="MDTIEIEAKTIDEAIEKACTEFNVSREKLNIEIISEGSAGFLGLG
                     SKKAKIKASRLSLGLDLEAPFELLKKEEVVRPSPVREELQPPAPPAPLVPPPSVREVAA
                     VPVAPAVPEVQGDNASLAQRAKSLLEGILQRMHLDFTVSLEETPDTIILTINGDGGGLL
                     IGKRGQNLDAIQYIVNKAATKIHDERKLIIVDTESYRQRREESLNSLAREFAEKVKKTR
                     KPVTVGHMNAHDRRIIHLALQNDDTIVTKSRGEGEYRKIVILPAKIANREPRP"
     CDS             complement(10757..12403)
                     /locus_tag="ctg48_12"
                     /transl_table=11
                     /translation="MDKKAILAIVLSSAVLLVYQLFFYKPAQPSKPQPATQGKGTTTVV
                     QPKSTVSPSTSVSPSPTVGAVASQGGMPLQAEDANVKEQEISIDTPLYSAVFTTRGGAL
                     KSFALKKYRETIAADSKPVELVDVKETMPYPLSLSFPASSIDVSPAALYQADATALDLK
                     STAEPRRLTFVQTWPGKIKIEKIYTFYPQKYVIEMEVRAYNLSDVPLSQEIVLFWNQYV
                     NPNVKEDSYGHVGPVSYIAKDVERESVKKMETAKVLGPDVTWAGFESKYFLAAMIPQNP
                     SLTSLLLSKEGTTNMVSTGLKGPKNIIPPGQAGFFSYTLFLGPKDYDILKSLGAGLENA
                     IDFGSWLKWLAMPLLVSLKFLYSYVQNYGIAIIILTILIKILFWPLGNKSYKSMKEMQK
                     LQPKMQALKEKYKDDKARLSQETMALYKAYKVNPMGGCLPMIIQIPVFFGLYKALLYAI
                     ELRHSPFFWWIQDLSEKDPYYITPLIMGATMFIQQKMTPVSGDPTQAKIMLFMPVVFTF
                     MFLTFPSGLVIYWLFNNLLSIGQQYYVNKRA"
     CDS             complement(12680..13042)
                     /locus_tag="ctg48_13"
                     /transl_table=11
                     /translation="MKKLTLKKNERIRKRKSYLQIYQRGKRSYSRHFTVVVAENDLGVL
                     RLGVAVTKKIGNAVKRNRTKRLIREFFRLNKDRFKASSQDIVIIAKGNTSLMKYADVCR
                     ELGVLLTKKPQNIPQE"
ORIGIN
        1 acataaggat ggccatttct catgtggaag tgtgaaatgt ctccccaagt tcaaaagggt
       61 acgctgacgt acctgtttgc cgatacgctc cgaagaaacc agtcggtagg taaagtaaga
      121 ttcccctggc gtagtcgcac ttccggtttt tgtttggcgg ataaacatgg aggcggtata
      181 caggaatcgg gcctcaatgt caagtagcca atatatttta tggcactaca ttggcggttt
      241 tgacacccct ccattgattt tattggactt ttcaccatcg aaatgccaaa tatcgacgaa
      301 aaatggagca tcggtgttaa agatggggta ggaccgccaa ttcagcctgc actctcaata
      361 attaagattg catatttatg atgggagggc agagatggtc tttttatcgg gtaaagtaag
      421 gctggtgtga aagaatgcgc ctcagtcgga tgaaccgaga agatgaatgg cttccaggat
      481 gcgcgggcgg gtttccaggg ggaggttgtt ctgccgggct ttttccacga ggtcccgtcc
      541 catcagcgcg gcgccgaggg cgccggtgat caggggattt tcggggatga ggagatcgcc
      601 gttaaggtac tcccgcaggg cggcgataag ccccttgttt ttgctgccgc cgcccgtcag
      661 aacgacgggt ttttccaggc ggagccggtg ggccatccgg ctgatccggt ccgcaaggga
      721 acgatggacc ccggccagga ggtccgggac gggaatcccc tgggccaggc tggaggctac
      781 ttcctgctgg gcccagatcg tacagaggtt gctgatcgtt gccggggagg tgctctgcag
      841 ggagagatcg cccaccgctt ccagggggat gttcagggta tcggcgatga tttcgatgaa
      901 tcggccgctg ccggcggcgc acttgtcatt catgatgaag tcgtggggac gcccctggct
      961 gtcgatgcgg atggccttgc tgtcctgtcc cccaacatcg atcaccgtcc gggcttcggg
     1021 aaagaggaag tgaattcccc gggcatggca ggtgatttcc gtgatctgcc ggtcggcaaa
     1081 gggaacgttg atgcggccgt agccggtggc gacaacgtag gagatggctt cgaaggcgag
     1141 tcctgcctgg tcgagggctt cctccatcac cttgttggcc agcctgcgct gttccgcacc
     1201 cgttggtccg atgaccgagg cggcaatccc ctcgccgagg atcaccgcct tggtcatggt
     1261 tgaaccgata tcaatgcctg cgaagtattc cgccatttcg cacaatcatc tcaggccgcc
     1321 cggttggtcc ggacggattc cagggtttca atgaaggcgt cgatcttgtt gcgggtatca
     1381 atctcggagt aggaagtgat atcgatgatg tcgctttcca gaatcaggga aggcacctcg
     1441 cggttggtct tctctttctg tcctccggaa cccatcatca ggatcgggat gggcttgtag
     1501 atcttggcca gctgcttcaa ctgccagatg aggccgacgt gccaggtccg gcaggaaaag
     1561 gcctgatgga caacgattcc gtcgatgtcg tattcctcga cccagttgat gaggcgctcc
     1621 acgtcgggtt ccgatccagg gcgtttccgg gccttgtcgt accagaaggt ccagtatccg
     1681 agccaccgcc aggcgagacg ctccacggga tcgctggtct tggggagatc catttcatcc
     1741 aggggcatga ccaagcgata ggtggtctcc acggggaacg tggccccctt gctgttgaag
     1801 tagttgaaat cgttcagggc gtaccaggcg ggaagtccgc cgccccacat cagccggtac
     1861 ttttcctctt cgacttcgcc ttcgcccttg gcgatctttt ccttcaattc cttcttgaga
     1921 tcggagaaga aattgtagga ttcctgggtg gccatcatga agttcagggg aaccatcgtg
     1981 ttcatggcgt cgccggttcc catggggctt ggcttggcct tgcgcagctc ataggtttcg
     2041 tggaccaggt tccagatgcg gtcgctcaga tccacccgct cgctcaggcg gtcgtaatcc
     2101 atcttctttc ccgtcatttt ttccacaaag gcgatgaggc cgcgcaattc cttgacaata
     2161 taacggtgat agtagcccag gatttccctg tgatcgcggt ctctctggaa cagggggtag
     2221 gggagatcga tgttgtagat cgggatgtcc ggcatgaact gctgtgaggc ctggtaccac
     2281 ttggccctcg ggtcacagag gatctgaccg gtttgaatca tgaagtcagg cctcgcctgg
     2341 ccgccccagg gggcgtcggg gggcgtctca cccagctcct cgcgccactg gtcatagccg
     2401 atgccgcaga gggcgtaggt gcacagggag cgggagagtc ccagcgattc cgcccgctgc
     2461 atgaagcgtt cggcatcccg cttcgcgccg cagacgcctg cgaaattttc ggtccacacc
     2521 ggcaggacat ccatcgcccg gagaatctcc tcatggtggc agcagatgaa cgtataagcc
     2581 agtttatggg tgccttcctt cctggcccta tttgtaccgg cgataaattc cttaaccatg
     2641 gggccgatgg aggcggctgc atgggttgca gtcattcttt tctggggttt cttttctttc
     2701 ttttcttctg tcattgctct gtcctcctta tgccggcatg gccgctagcc gatcatttcc
     2761 aggaaagcct ggatgcgtgt cttcaattgt ccgatggttc ccgccgaata ggtgtcttca
     2821 agacagagga gcgggatatt gagggattcg tagtaggcct ttctcgccgg gacttcgaat
     2881 ccaaagggat cgcagtactt gtacaggtaa agaatggcgc cgttggcggc aaactccttt
     2941 gcccgtgcgc cgatgtcgcc aaagcgatcc tcggcgtcgc cggcaaaggt gccggcggtg
     3001 ttttcccggt atgtcttggg acaattgagc ttgtccaggt agcgacgggc cagggaggcc
     3061 accggatcgc cctcttcatc ggcaaggggc cacagatccc gcgtgcccat gcaggtcgtg
     3121 tcgatcacca cattgccgcc gttttcttcg acgatgccgg aaatggcgag atgatccacg
     3181 caggggccgt ccaggagaat ccggggacct ttcttgaccg gggcttccgt ccgcctgccc
     3241 agttcggcca ggacttcgtc gaagagggaa ttggcttctt caaccggtag gcttgccccg
     3301 acggccagaa ccttctgcag ttccgctgcg gaaatcagcg ggggatcggc ctttttaaat
     3361 tcatacaggg cccgggtttt ggtccgattt tcattgtgca gccggatggc ttcctgaaga
     3421 tctgcatcgg aaatgggctt tcccgtgaaa tcttccaggc tcttcctgta cgactggagt
     3481 tcctccttga aatattcgtg ggaagattcc ttcacgacac tgggcgtatt gacgaagtga
     3541 aagtagggaa aattgaggga ataactccac acgctgtagc ttctcaccat gctgtcgcat
     3601 ccgtggggga tcacgatgcc ggagagaaag tcgtatcggt ccttgacgca gagatcaaaa
     3661 caactccgga taaaggggca gacgattgtc tccaggaggg aatccccttt ggtgatgggt
     3721 tcgtggatat caccgcgaat ccggaagggg atacatccgg cagccgtgat aaattcgagc
     3781 ggaacaaaag agcagatgta accgatcgcc tttttcccgc cggcgcatag ttcctttgcc
     3841 cggattccat aattttcgta ctgtctttcc acctctgcca tgatgttgtt cttcgtatcg
     3901 ctcattaaac tccctcctct tttttaacga cagaacttag tatttccaac ccgtcgttct
     3961 tcagattcct tgcgacaggc ttttctcccg cgccagcagt gccgcgccaa gggcggcggt
     4021 gaacagggga ttcgaaggca ctctgaccgg gatacgcagg ctgtcctcca gggcctggac
     4081 cagaccggcg tctaccgccc cgccaccggt cagaaccaca tccggttcga ttcccactcg
     4141 ttccgccagg ctggcaagct gggcagccaa tgcccgatgc acaccggcca gaaggtcttc
     4201 cttggccacg ccttccgcga tcagggatac ggcttcggat tcagaaaaaa caacacaccc
     4261 cgtgctgaag tccaccttgc ttcgggattt cagagaaagc ggaccgatct cctccaactt
     4321 gacccgcagg accttcgcca tgatcagtaa caaacggcca ctgcctccgg cacatttccc
     4381 gctcaaaagg aaattatgga catgaccctc gccatccatg cgcaggactt tgctgtaaag
     4441 atccccgcaa tcgagaacgg ttcgggctga aggaagtaga aaggcgaccc ctctgccctg
     4501 acaggtgata tccggttttt cttcatcggc gaacaagacc tgctttgctc cgtaacctgt
     4561 ggccatgacg aaggataaac ccgaaggtgc tattccggcc ctggacagca gggtgtctct
     4621 gacctgctcg gcggctcccc ggtaatctcc gccggaggga caggcaaagg aagcctgaac
     4681 ggattgatcc tccagcagga cccctttcgc ggaaaaagat ccgatatcga tacccagaaa
     4741 aaaagccatc gtttaactca gcgtgattat ttgtcgttct tattgcttaa aactctttgc
     4801 ggtgcgtctt ctgaaagcgg aatcataggg gaagccgtct actttatcgg gaaagatatc
     4861 gagtgcaaac gcgttgcttc cggccagcac ttcgctatcc tttgaaagga tcagacggat
     4921 ccgcctttgc ctttcctggg gaagcgatgc gggatctttg actcgaggcg caaaagatca
     4981 agccgacaag ccagtgactt aaaacaaagt atggggagtg tcaatcaaaa aatcagagag
     5041 atgccccgtt gttcatggct tggacaggag gggttttctc cggtggctcg ggagcgtccc
     5101 cctgaaggag agtagcgtta gatccggaaa agtttcgact ggtcacggca taaaatttgt
     5161 tgtaggtatc ttgtcgcaag ttaagttttc tggaaagaat gatcattata aaatcctgaa
     5221 aatagagaga atttaaaaat gacggaagaa gggttggaga aaaagatccg tgccgttgcg
     5281 gacatgatct gcgaggccgg aaaggtcgtg gtctttaccg gggcgggcgt cagcaccgag
     5341 tcgggcattc ctgattttcg cagtccggga ggattatggg accggtttga tcccgatgat
     5401 ttcacgatcc agaagtttct ccgcagcgcg cagacgcgaa ggaaacagtg gcggattctg
     5461 atcgagggag gcgcttttgc ggaagcgcaa cccaaccggg cccatctggc cgtggccgaa
     5521 ctggagaaac tggggaagct gcggtgcgtc attacccaga atattgataa tctgcatcag
     5581 aaggcaggaa atgcgccgga aagggtgtac gaactccatg gcaacatgcg ctggcttaaa
     5641 tgtctgagct gcggcgaccg gatttccgta cccgacatgc ttcagaggac ggcattaaga
     5701 gaattggatg gatttccctt ctgtgagcga tgccagggcc tgttgaaacc ggatgtgatt
     5761 ttttttggag aggctcttcc ggaggacacc ctgcgggagg cgacttatga ggcgagcaac
     5821 tgcgacctga tgctggttat cggttcttcc ctggtggttt atccggcggc ttatatgccc
     5881 caatatgcaa aagatgtcgg tgcaaagctg gtgatcatca atcgggaaga gacgccctat
     5941 gatgcggagg cggacatcct cctccagggt ggtgcggggg agatgatgac ccggatcctg
     6001 gcggcggtca aggatcaaat cgatctatag ttttttccaa tcggtatttc aggagtccag
     6061 ggttgttaag ccctcccgga tggcatattt gattaattcg gccacgctgc cggctccaag
     6121 tttctgggca atgttccgcc ggtgggtttc cacggtcttc acactgacac gcagggtctt
     6181 ggcaatctgt ttggccgtca tgccttccgc aagcagctgg agaagttctc tttcccgcgc
     6241 cgtcagttcc gaggagacgg agaccgatgg tttcgcttcc ttgtgcacat aatttctaac
     6301 gacgatatcg gtaatggtgg ggcttagata ggtgtggttt tccaggacgg tgtgaatggc
     6361 gctgacgagt tcttcaaagg cgcagtcctt taagagatac cctgcggcgc ctgcttccag
     6421 catgcctacg acaaaatggc gatcggtatg catggagagg gcaatgactt tgattccggg
     6481 aaagtcggag gtgatcctcc ttgtggcatc gatgccgttc atgtccggca tggagatgtc
     6541 catgataatg acctgcggat gcagctcttt ggccagggtt attgcttttt gtccattttc
     6601 cgcctctgcg acaacattca tattcccgtg gttttcaatg agaatacgta agccgtcacg
     6661 gactattttg tgatcgtcgg caagaagaac attgacagcc attttccctt tctccttatt
     6721 attttaattt gtcattttta ccatagaagt gctgggccgg tcacactttt ttatacctcc
     6781 tctatcatta aaaaaggaga aaaaaataca tcctcaagaa acattgtgac agtgcctgaa
     6841 aggtcttgcg gagagtgaat tttatcacaa tcttaagtct tgcttatatt acagtccgag
     6901 tttcgttcca ataaggggaa aaccgtatta tgtcagggaa aaattactga tatttgattc
     6961 agtcaaagga ttagaaaaat tagggcggtt attgaacttc ctaccaggta aagaacgcat
     7021 tgtaacgatt agggaaatgt gctatttttt gtcatatttt ctcagggcgg gtgtattttt
     7081 tatgtcgaaa cgggaatatc cggattgccc tcgcgtgggc gttggcgcaa ttgtcgtccg
     7141 ggaggggagg attctcctgg taaaacgcgc ggcggaaccg aacaggggat tgtgggccat
     7201 accgggaggc tctctgaaat tgggggaaac cttgaaggaa ggagcggaac gggaagtttt
     7261 ggaagaaacg ggcattgtcg tggaggcaaa gcaccccgta tatgccttcg acctgctgga
     7321 aagggaccag gagggtagct ttcgctttca tttcgtcatt gtggatatgc tggccgatta
     7381 tattcgaggg gacctgaaag ccgccgatga tgccctggat gcccgctggc ttttaccaga
     7441 agacctggaa caggttgatt tgtcgacttc cacggtaaaa attctcaggc acatcaaatt
     7501 ttatggttag tctttttttc cggacaggga gaggcctttc cgaatcgttg agccgttgtc
     7561 gaccatctgc tatcgaaggg acatatcttg gaggaggtgt cgaaaatcat gaaagtcatt
     7621 tatcatgatg atttttgtca atgctacacg acggatccgg ccgcggcttc aggcaggatg
     7681 gaagccgttg tggaagcgat cagcggccgg gttgattttc ttaaagctga accggccgaa
     7741 gaatctgata tttcggccgt tcataccgaa tcgcacattg ctctggttga aagggaaggg
     7801 ctctatgaca ttgccgcgct ggctgcaggc ggggccatta tggctgctga aatcgggatg
     7861 aacgaaccct gttttgccct gatccggcct ccgggtcacc atgcctccgc cggcagcgcc
     7921 tggggattct gttatttcaa taatatggcc attgccttgg aaaaattgaa aagggaagga
     7981 aagatcgtca aggcctttct tctggacttt gatcttcatt tcggtgatgg tacggtgaat
     8041 atcctggaac ggaaggaata tgtaactatt tataatcctt catcctgcga caggaatgaa
     8101 tatctcaggg atattgaaaa aaaattgtct tcggcgtctt tcgatatcat cgccgtttcc
     8161 gccgggttcg ataatcacag ggaagactgg ggtgggcttt tgacgacgga ggattataaa
     8221 accatcgggc atatggtctg gaaaatctgc cagaagaagc ggtgtggatg cttcgccgtt
     8281 cttgaggggg ggtataacca tcgggttctg gggacaaacg tcctggcgtt tcttgaaggc
     8341 atcgagggcc tttgacgaaa ttccttttaa accaaattgc atttcgaagg ataacaaggc
     8401 ggcaaggagg aagcaatgca ggcgtataga aaatacgttg aggagccgac gacgaagcca
     8461 acgcagttag ccgaagaaag gcaacttggt gtgggatcat ttcccgatgc agaaggatga
     8521 gaagatgcga tccaggatct cttccgtggc cgtttcccct ccgatttctc caagcgcttc
     8581 cagggcttcc cgcacatcca gtgcagcaaa ctcaggagat aaaccgtcct cgaggctgtt
     8641 tcgcgcctgg gcaacaaatt tcaacgtttt ctccagagcc attttctgcc gcaggctgga
     8701 aatcacaatt tccccatcct gaattcccgc cctgtccatg accagacggc ggatcttctc
     8761 tttcaaagcc gggatgccct ctccgaattt cgcggagatc cagagcggag acgttccggg
     8821 aaaacaggct gtgatttctt cttctcccaa gcggtgatcc agatcggttt tgttgatgac
     8881 gggcagtacg ttataatccg cgagccgttt caggagatcc cgatcttcct ccgtcagaga
     8941 gctgctcccg tcaaagagaa ggatcacgcc atcggcggcg gccagttttt cccagaccat
     9001 ccggatccct tcctgttcaa tcctgtcttc cggaggacgg atgccggcgg tgtcaatcag
     9061 ccggatggac agcccctgga tgttgattgc ctcttctatg aaatcgcggg tcgtccccgg
     9121 tatcggcgtg acaatcgccc gcttttctcc cagaagacaa ttcagaagac ttgattttcc
     9181 cgtattcggt ttaccggcaa tcaccacggc agcgccatgg cggtctattt tcccctgatc
     9241 ataggtccga agcaggatct cgatgtgttc ggaaagggac tggagttttg ccggcgtttc
     9301 agcagcaggg gattccacat cctccgaaaa ttcgatctcc gtctccagca gggccagaat
     9361 atcgatcagc attttccgca ggacatcgat cttttgtttc aacttgcctt taaggtgcgc
     9421 cacggcggtt tcgagccccc gcgaggtgcg ggccgaaatc acgtcgccga tcgcttccgc
     9481 ctgggtcagg tcgatccggt catgaagaaa ggcccgccgg gtgaattccc ccggttcggc
     9541 aaggcgggcg ccggcctcaa ggaccgccga aaggacggct tcaagaatca ggatgccgcc
     9601 atggcagaaa atttccaggg tatcctcgcc tgtgaaggag gagggggctt tcatcagact
     9661 gaccaggact tcatcgagaa cctgtccggt cgtcggagcg atcaggtctc catgataaag
     9721 tcggcggggt tgataacggg atattcgatg agaggattga aagaggcgaa aaaggatgct
     9781 ttccgacttc ggcccgctga tacgaacgat tcctataccg ccgacaccgg cgggcgtagc
     9841 gatggcggcg atggtgtctt ccagcatggc gatctttcag acttaaaaag gggttcgtac
     9901 ctcttttttt atggccgagg ttcccggttg gcgatctttg ccggaagaat cacaattttg
     9961 cggtattcac cctcacctct gctcttggtc acgatggtgt cgtcattctg aagagccaga
    10021 tgaatgatcc ggcgatcgtg ggcgttcatg tggccaacgg tgaccggttt ccttgttttt
    10081 ttcacctttt cggcaaattc cctggccagg gagttcaagg attcctcccg ccgctgccga
    10141 taggattcgg tatcaacgat gatcaatttg cgttcatcat ggattttcgt ggccgccttg
    10201 ttgacaatat actggatggc atcgagattc tgtccccgtt tgccgatgag caacccgccg
    10261 ccgtcgccgt tgatggtcag aatgatcgtg tccggggttt cttcaaggct gacggtgaaa
    10321 tcaagatgca ttctctgcag gatgccctcc aggaggcttt ttgccctctg ggccagagat
    10381 gcgttatctc cctgaacctc aggaactgcc ggggctacag gaactgccgc gacttcccga
    10441 accgagggag gcggaacgag cggagcgggt ggagcgggcg gttgaagctc ttccctgacc
    10501 ggtgagggcc ggacgacctc ctcttttttt aaaagttcaa agggcgcttc caggtcaagg
    10561 cccagagaca atcggctggc tttgatcttt gcctttttgg accccagtcc caggaatcct
    10621 gctgaacctt ccgaaataat ttcgatattc aatttttctc gggaaacgtt gaattccgtg
    10681 caggcctttt ctattgcttc atcaatggtt ttggcttcaa tttcaatggt atccattttt
    10741 gcaccctgag tcgtagttat gctcgtttgt tgacgtaata ctgctgtccg atgctgagga
    10801 gattgttaaa cagccagtag atgaccagcc cggagggaaa agtgagaaac atgaaggtga
    10861 agacgacggg catgaagagc atgatcttcg cctgggtagg gtctccggac acgggcgtca
    10921 ttttctgctg aataaacatc gtcgccccca tgatcagcgg cgtaatgtaa taggggtctt
    10981 tttcagacag atcctgaatc caccagaaaa agggagagtg gcgcagttcg atggcataca
    11041 gaagggcctt gtagaggccg aaaaatacgg ggatctggat gatcatcggc agacaccccc
    11101 ccatgggatt gactttatag gccttgtaaa gcgccatggt ttcctggctc agtcttgctt
    11161 tgtcgtcctt gtatttctct ttcagcgcct gcatcttcgg ctgaagtttc tgcatttcct
    11221 tcatggattt gtaactcttg tttcccagag gccagaagag gatcttgatc aggatcgtca
    11281 ggataataat ggcgatgcca tagttctgaa cataggagta gaggaacttc agggacacga
    11341 gaagcggcat ggccagccat ttcagccagg aaccgaaatc gatggcattt tccaggcctg
    11401 ctccgaggct cttgagaata tcgtaatcct ttggccccag gaagagagtg taactgaaga
    11461 aaccggcctg accgggaggg atgatgttct tggggccttt cagaccggtg gagaccatgt
    11521 tcgtggtgcc ttccttggaa agcaaaagac tcgttaaaga gggattctgg ggaatcatgg
    11581 cggcaagaaa atatttgctc tcgaatccgg cccaggttac atccggtccc aggactttgg
    11641 cagtctccat cttcttgacg ctttcccgtt caacatcttt ggcaatatag gataccggac
    11701 cgacatgacc gtaactgtct tccttgacgt tcggattgac gtactggttc cagaagagca
    11761 caatttcctg gctcaggggg acgtcggaga ggttataggc ccggacttcc atctcgatga
    11821 cgtatttctg ggggtaaaag gtgtaaatct tttcaatctt gattttaccc ggccaggttt
    11881 gaacaaaggt caaccggcgg ggttcggcgg tcgacttcag atcgagggct gtcgcgtcgg
    11941 cttgataaag tgcggcaggg gaaacatcaa tgctggaggc cggaaaggag agactcaatg
    12001 gataaggcat ggtctccttg acatcaacaa gctcgaccgg cttggaatca gcggcgatgg
    12061 tctcccggta tttcttcaac gcaaaggatt tcagcgcgcc cccccgagtg gtaaatacgg
    12121 cggaatacaa gggggtgtcg atggaaatct cctgctcctt tacattcgcg tcctctgcct
    12181 ggagaggcat gcccccctgg ctggcaacgg cgccgacagt cggagagggg ctgacggacg
    12241 tcgagggtga aacggtggat ttcggctgaa caacggtggt tgtgcctttt ccctgagtag
    12301 cgggttgtgg ttttgagggc tgcgccggtt tgtagaaaaa cagctgataa accaaaagta
    12361 cagccgagga aaggacgatg gccagtatcg cctttttgtc cattaagaac ctccgtttat
    12421 ttcgtagtta cggtatgccg gcatttcccc gccattatct aacgcaccgg atcgtagccg
    12481 cccggatgga atggatggca tttgagcaga cggagcaatg caagccggga acctttcaga
    12541 ggaccatgtt gccgaagcgc gataaccgca tactcggagc aggaaggata aaaacggcaa
    12601 cagggggcaa acagggggga tatcaatatc tgataaatgc ggataaggaa aataaagatc
    12661 ttcgcaaaaa atttcctggt cattcttgag gaatattctg aggttttttt gtcagcaata
    12721 cacccagctc cctgcagaca tcagcatatt tcattaacga tgtattacct ttggcaatga
    12781 taacgatatc ctgagaagat gctttaaacc tgtctttgtt cagtctgaaa aattctcgaa
    12841 tcagccgctt ggtcctgttt ctcttgacgg cattcccgat cttttttgtt acagcaactc
    12901 caagccttaa aactcccaga tcattttcgg cgacaacgac cgtgaaatgc cttgaataac
    12961 ttcgctttcc acgctgatag atctgcaggt aactttttcg cttcctgatc cgttcattct
    13021 ttttcagggt cagctttttc atctgctgca gaccaggcaa tagttcaggg cgttcgcagg
    13081 caacctctct ccttccggga ggatcaaacc gtgagacgct tcctgccttt ggcccttctt
    13141 cggctcagta ccagcctgcc ccctcttgtg gacatcctgg tgcgaaatcc atgggtcctt
    13201 tttctcttca gattgctttt gttccttaaa ttgattttca taaattcatc ccgtgagcaa
    13261 attagtaagc cactccataa atcatagagc cgttttgttg tcaagattaa aatcccgacg
    13321 gcatccggct gttgataaaa gaaggggctt gtctttgttc gaggaaccgt ttattccatc
    13381 ggaaagaagt tcccgaggaa taaacggttg gaacattctg ccagacattg taaaatgatg
    13441 caaaaaagaa tcatgacttg ctgatgttga aaagatcacg cttatctaaa gtaactccga
    13501 tgtcggacat atttctctgg gggtagagca tgattggacg gaagctggaa tttttaaaga
    13561 tcctgggagt tgcgctggtt gggctgctgc tggccggaat ggcatgccgg cctgtttttg
    13621 caggtgaacc ccaggcaacg atcagtttga aggagagcat cgaggaggcc ttgaggaaga
    13681 gcgcaaccct tcactcggcg aaggagggcg tccaggttgc ggaagcccag cgcaaggagg
    13741 ccttcaccgc ttttctgccg aggttcagta cctcgtacag ttacaaacgc ctgcatgaaa
    13801 accccgaatt caactccttt atcccacttc ttaacaacag gccttttacc gttcagtccg
    13861 gaacaaagga caattataac tgggccgttg aagttcgcca accgctcttt tccggcgggg
    13921 ggattctggc caactatcag gtctattcgg tcggcctgga ggcggtccgc caggaggagc
    13981 ggacagccgt tctagatctg gtcctggaag tccagacggc ctacctgaac atcgtcaagg
    14041 cggaaaagct ccgggaggtt gctgcgtcgt ccttggatcg cctgaccgct caccgggaca
    14101 tgacccggca tttctacgag gtgggcgtgg ttccccggaa cgacctcctc tatgccgagg
    14161 tggaactggc caacgggcag caggcgctgc tccgcgcgga aaacggagtg gcgatggcga
    14221 aggcgaaatt caacacgctg ctgagacgaa ccatggacac gccggtttcc gttgaggaca
    14281 ttctggccta tgcgccttac aacaaagcct ttgacgactg tctgaaaacg gctctggaga
    14341 aaagaacgga gatcaaaacg tataatttca aggtggagca ggcccagaaa tccgttcaac
    14401 tggccagaag cgaatattac ccggcggtca atctcgtggg caattattcc cggtttggcg
    14461 acgatcccgg ggtttctggc agcccttatc aggattcgga aagctggtac gtcatggcca
    14521 tggccaactg gaacttctgg gagtggggaa aaacaaaaaa tcgcgtcgat gccagcctca
    14581 gcagagaacg tcaggcgaat gacgccctgg tgaacgttcg cgattccatt tccctggagc
    14641 tgaaaaatgc ctggctgctt ctgaaggaag cggaaaaaca gattgccgtc agccggacgg
    14701 ccgtggtgca ggctgaagaa aattatcgga tcaatacgga acgataccg
//
