LOCUS       c00054_NZ_LDYG..       13930 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_LDYG01000054.1 Bacillus coahuilensis p1.1.43 ABFP000054, whole
            genome shotgun sequence.
ACCESSION   NZ_LDYG01000054
VERSION     NZ_LDYG01000054
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 00:33:34
            Original ID  :: NZ_LDYG01000054.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13930
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13930
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[7726:12104]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      7727..12104
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..13930
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..13930
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             2..433
                     /locus_tag="ctg54_1"
                     /transl_table=11
                     /translation="AALRPFACAPGFCVRILVRCVLLFLFCVRISVACVRLSAVCVHFQ
                     IFRAGTAPSCAVARHYCAVRQSFCAANDQSCADTITLVRQSLDLVRLIYHPVRIHTQMV
                     RVATNPVRDPFAGHPSHPSHPSHPGLPGLPILPQLNISP"
     CDS             complement(537..2141)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg54_2"
                     /sec_met_domain="GATase (E-value: 2.8e-63, bitscore: 202.9,
                     seeds: 97, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVTIQKFDPYLN
                     VDPGTMSPYQHGEVFVTSDGAETDLDLGHYESFIDINLNQYSNVTTGKVYSSVIRKERR
                     GDYLGGTVQVIPHITNEIKERVFRAGHETNADVVITEIGGTVGDIESLPFLEAIRQIKS
                     DIGRDNVMYIHCTLVPYIKAAGEMKTKPTQHSSKELRSLGIQPNIIVVRTEQPISQDMK
                     EKIALFCDIDSQSVIEALDADTLYAVPLALQAQHMDQIVCDHLKLDSKEADMTEWNALL
                     ERVRNLSTKTTIALVGKYVELQDAYISVVEALKHAGYNYDADVKVKWLNSELLTTENVE
                     ESLSDVDGILVPGGFGDRGVEGKIVATQFARERGIPFFGICLGMQLASVEFARNVLNME
                     GAHSSELNPETAYPIIDLLPEQKDVEDLGGTLRLGVYPCKISQGTKALEAYGEEVIYER
                     HRHRYEFNNHYREKMEEAGFVFSGTSPDGRLVEIIELTDHPWFVACQFHPEFTSRPTRP
                     QPLFREFVNASLLFGEK"
     CDS             complement(2395..2856)
                     /locus_tag="ctg54_3"
                     /transl_table=11
                     /translation="VNIRELSQDELKEVSFIEITNAILLEKKEAIPFNEIVKDIQKILE
                     ISDQEMKRKIAQFYTDMNIDGRFLAIGDNKWGLREWYPVDQIEEETAPTIKVRKKKKKS
                     EDDEDLFDDIDEEELDLDELDDFEEDEDLAEDEDLEDFDDTDDEEIIRG"
     CDS             complement(3407..6646)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) B12-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MeaB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MM_CoA_mutase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg54_4"
                     /sec_met_domain="B12-binding (E-value: 5.6e-14, bitscore:
                     42.1, seeds: 91, tool: rule-based-clusters)"
                     /sec_met_domain="MeaB (E-value: 1.5e-49, bitscore: 158.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="MM_CoA_mutase (E-value: 1.4e-147,
                     bitscore: 482.0, seeds: 314, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIYQPKHSIRFVTASSLFDGHDASINIMRRILQSSGAEVIHLGH
                     NRSVEEVVNAAIQEDVQGIAISSYQGGHVEYFKYMYDLLQERGAGHIRIYGGGGGVIIP
                     REIKELHEYGIARIFSPEDGRTQGLQGMINRMLKECDFLTVGGEIAEQVNDVSPNNVNA
                     VSKLITLAELHVREATDVAATAESILTSVKKHEKDVPVLGITGTGGAGKSSLTDELIRR
                     FLHEVPEKRVAILSIDPTKQKTGGALLGDRIRMNAIFHSRVYMRSLATRGSKSELSLAI
                     RDAVSIVKAAGFDWIIVETSGIGQGDAEITEIADLSMYVMTSEFGAPSQLEKIDMIDFA
                     DFIVINKFDRKGSEDARSQVQKQYQRSRMLFDQELSEMPVYGTIASQFNDPGTNALFAA
                     ITEKMNERFRSGWTTAFSKHEVVEKQNVIIPPNRRYYLRDISEAVRGYHRKVAEQEKVA
                     RQLFQLQGTEDLLQEVPESFNRVKESVQESLNAESKEILSKWEETKRLYEQDQFVTNIR
                     GKEIVTELKTKSLSGLSIPKVVLPKYKDYGEIIKWVYKENVPGAFPYTAGVFPFKRKGE
                     DPKRQFAGEGTPERTNRRFHYLSKDDDAKRLSTAFDSVTLYGEDPAHRPDIYGKVGESG
                     VSICTLQDMKKLYDGFDLCAPSTSVSMTINGPAPIILAMFMNTAIDQQVNKREEEVGRP
                     LTLEEFMETKEATLKVVRGTVQADILKEDQGQNTCIFSTEFALRMMGDIQQYFIDHEVR
                     NYYSVSISGYHIAEAGANPISQLAFTLANGFTYVEYYLSRGMDIDSFAPNLSFFFSNGL
                     DPEYTVIGRVARRIWAIVMKDKYGANERSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQA
                     LMALHDNCNSLHTNAYDEAITTPTEESVRRAMAIQMIITKEHGLTKNENPLQGSFIIEE
                     LTDLVEEAVLQEFERINDRGGVLGAMETQYQRGKIQEESMHYEMKKHSGELPIIGVNTY
                     LNPNPPSEEEMDNMELARASKEEKDAQIRHLREFQQEHEDKTRVALRNLQETAMSGGNI
                     FAELMECVKVASLGQITQALYEVGGQYRRNM"
     CDS             complement(6621..7277)
                     /locus_tag="ctg54_5"
                     /transl_table=11
                     /translation="MKRPVKASVKDEKLIEIRRNQIIRGAVTLFKEKGFHRTTTREIAK
                     AAGFSIGTLYEYIRTKEDVLYLVCDNIYDGVKEKLSEMDVNGGTISQLKQAIAYYFHLM
                     DELQEEVLVMYQEAKSLSKDAIPYVLQKEMEMVDMFQVLLRNCVRSGEIELDEQQIYVT
                     ANDIFVQGQMWAFRRWAFQRRYTIEEYIELQTELVCHGIECKRGKVKHGNLSTKA"
     CDS             complement(7727..8869)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_6"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.1e-56, bitscore:
                     179.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2.8e-29, bitscore:
                     91.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2e-47, bitscore:
                     150.4, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFKLSEEHEMIRKMVRDFAVNEVAPTAAERDEEERFDRDIFDKM
                     AELGLTGIPWPEEYGGIGSDYLAYCIAVEELSRVCASTGVTLSAHTSLAGWPVYKFGTE
                     EQKQKYLRPMAQGEKIGAYGLTEPGSGSDAGGMRTTARLEGDHYILNGSKIFITNGGIA
                     DIYVVFALTDSEKKQRGGTTAFIVESGFEGFSVGKKEKKLGIRSSPTTEIIFEECKVPK
                     ENMLGNLGEGFKIAMMTLDGGRNGIAAQAVGIAQGALDESIRYAKEREQFGKPIASQQG
                     ISFKIADMATSIEASRLLTYQAAWLESEGLPYGKESAMSKLMAGDTAMNVTTDAVQIFG
                     GYGYTKDYPVERFMRDAKITQIYEGTQEIQRLVISRMLTK"
     CDS             complement(8891..10027)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_7"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 8.1e-57, bitscore:
                     181.4, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.5e-30, bitscore:
                     94.0, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.5e-43, bitscore:
                     137.9, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFLFTEEQEMVRKMVRDFAREEVAPFVERMEAGEFPRPILSKMA
                     ELGLMGMTIPEEYGGAGMDFTSYIIAIHELSKVSATLGVVLSVHTSVGTNPILYFGSEE
                     QKQKYVRKLASGQYLGAFCLTEPSSGSDAGSLRTRAVLEGDQYRLNGSKIFITNGGEAD
                     TYVVFASTDPEKGTRGISAFIVEKDTPGLVIGKDEEKMGLHGSRTVQLTFEDMLVPASN
                     LLGAEGEGFKIAMANLDVGRIGIAAQALGIAEAAFDEAVEYAKERVQFGKPIAAQQGLA
                     FKLADMGTAIEASKLLVYQGANLRTEGRPCGKEASMAKLFASKTAVHVTTEAIQVFGGY
                     GYTEDYPVERYFRDAKVTEIYEGTSEIQRVVLGKHLLK"
     CDS             complement(10041..10892)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_8"
                     /sec_met_domain="HCDH (E-value: 9.6e-40, bitscore: 125.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 6.3e-71, bitscore: 227.7,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIKRVMVIGAGQMGSGIAQVCARGGYDVVLNDVKQEFAEKGLAS
                     ITKNIARSVDKGKMTEEEKEGVLSRLTLSTNLEDAKNVDIVIEAAVENMTIKKDIFSKL
                     DSIAPEHTILATNTSSLPITEIAAVTKRPEKVIGMHFMNPVPVMKLVEIIRGLATADEV
                     YQAVEDMSKSLSKVPVEVNDFPGFVSNRVLMPMINEAIYTLYEGVASKEAIDEVMKLGM
                     NHPMGPLTLADFIGLDTCLYIMETLHEGFGDDKYRPCPLLRKYVKAGWLGKKTGRGFYE
                     YS"
     CDS             complement(10917..12104)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg54_9"
                     /sec_met_domain="Thiolase_N (E-value: 2e-99, bitscore:
                     321.9, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 1.1e-47, bitscore:
                     150.6, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKTVIIAGARTPFGKLGGALSSFTASELGGKAISAALERAGVAK
                     DQIDEVIMGNVLQSGQGQIPSRQAARHAGIPWEVKTETINKVCASGMRSISLGDQIIRA
                     GDESIIVAGGMESMSNAPYAIPKARWGLKMGDHQVKDLMLHDGLMCCFNDVHMGTYGNR
                     TATKFEITREEQDRWSFRSHERAVIAMDNGTFAEEIVSVEVPQRRGEPVVVDHDEAPRK
                     DTSYEKLSALRPVFDHDGTITAGNAPGVNDGACALVLMSDEKAEELGKTPLATILAHAE
                     VGVPAEEFPETPGLVINKLLQKTGKSLDDIDLFEINEAFAAVALTSGRIAELDHDKVNV
                     NGGAVALGHPIGASGARIVLTLALELKRRGGGIGVAAICSGTGQGDAIMIEVPKA"
     CDS             12398..12712
                     /locus_tag="ctg54_10"
                     /transl_table=11
                     /translation="MKRTKHLPLTETVFYVLLSLLEPSHGYLILQKVEELSKGSVRMAA
                     GTLYGAIENLVKLGFIEPVESGDKRRKVYVITHLGLEVLQADQQRMSHMVLLAENALKQ
                     "
     CDS             12721..13338
                     /locus_tag="ctg54_11"
                     /transl_table=11
                     /translation="MMKRLRYFMDLDKEEEWLNEKAKEGNVLYQKSFFYHFKATTEKDP
                     TLKLDYRTFKHREDFEDYIALFEDSGWKHIAGSKRSGVQYFQRVSNEATGDIFSDSHSK
                     AERYKRYAQTSASLLFTYFILLIGLMSADVLHLQEMLQPKSLYYTPGLWEKEGADFWRA
                     FLFETPFAFFRGFAWLLFPIAIVIFFLSATISYIKYEKLVNQ"
     CDS             13416..13640
                     /locus_tag="ctg54_12"
                     /transl_table=11
                     /translation="MKMGDYLNTIAWFIVTCEFSFWIVILLGLYIRYSLGKKSLELIFL
                     ALTSLIDLALNVLTTLDLARGSTSTVAQC"
     CDS             complement(13721..13930)
                     /locus_tag="ctg54_13"
                     /transl_table=11
                     /translation="KRECRKEEKEGRRTPREPGAVQKRGEGSKKSAARAERGAEKGERK
                     QEERRASLARCRKEGEEARRAPPL"
ORIGIN
        1 tgcggctctc cgcccgtttg cgtgcgcacc gggtttttgc gtgcggattt tggtccgttg
       61 cgtgctactt ttcctgtttt gcgtgcggat ttctgtcgct tgcgtgcggc tctcggccgt
      121 ttgcgtgcac ttccagattt ttcgtgcggg tacagcccca tcctgtgcgg ttgctcgtca
      181 ctattgtgcg gttcgccagt cattttgtgc ggcaaatgac caatcttgtg cggatactat
      241 tactctcgtg cggcaaagcc tagatctcgt gcggctaatc tatcatcctg tgcggataca
      301 tacccaaatg gtgcgggtag ccacgaatcc tgtgcgggac ccatttgccg gtcatcctag
      361 tcatcctagt catcctagtc atcctggtct ccccggtctt ccaatcctcc cccaactcaa
      421 catctccccc tgacacctgg ccttacgatt tccaattccc cctgaccttc acatcctccc
      481 aataaattaa aacaaacacc gctacagcgt agcggtgttt gccctaacta ttcaaattac
      541 ttctcaccaa atagtaaaga cgcgttcacg aactcacgga atagtggttg tgggcgagtt
      601 gggcgagatg tgaattctgg gtggaactga caggctacga accatgggtg atcggttagc
      661 tcgatgattt ctactaggcg gccatctggg cttgtgcctg agaatacgaa gcctgcttct
      721 tccattttct cacggtagtg gttgttgaac tcgtagcgat gacgatgacg ctcgtatatc
      781 acttcttcgc cgtatgcttc aagcgccttt gttccttgtg aaattttaca agggtacaca
      841 ccaagacgta gcgtcccacc aagatcttct acatcctttt gttctggaag tagatcaatg
      901 atcggataag ctgtttctgg gtttaactca gaagaatgtg caccttccat gtttaacacg
      961 ttacgggcaa attcaacgga tgccagctgc atacctaagc agattccaaa gaacgggatg
     1021 ccgcgttcgc gagcaaattg ggttgcgaca attttacctt ctacaccgcg gtcaccgaag
     1081 ccacctggta caagaattcc gtctacgtca cttaggcttt cttccacatt ttcagttgtt
     1141 aaaagctcag agtttaacca ttttactttt acatctgcgt catagttgta gcctgcgtgt
     1201 tttagagctt ctactacaga aatgtacgca tcttgtagtt ctacgtattt accgacaaga
     1261 gcaatcgttg ttttcgtaga caggttacgt actctttcta gaagagcatt ccactctgtc
     1321 atgtccgctt cttttgaatc caattttaag tggtcacaca caatttggtc catgtgttga
     1381 gcttgtagag ctagtggtac ggcgtataat gtatcggcat caagagcctc aatcacggat
     1441 tgagagtcga tgtcacagaa tagggcaatc ttctctttca tgtcttgaga gataggttgc
     1501 tctgttcgaa cgacgatgat gtttggttga atacctaggc tgcgcagctc tttactacta
     1561 tgttgagttg gctttgtttt catttcgcct gctgctttga tgtaaggaac tagtgtacag
     1621 tgaatataca tcacgttatc gcggccgata tcactcttaa tttggcggat ggcttctagg
     1681 aatggtagag attcgatatc ccctacagtt ccaccgattt ccgtgatgac gacgtctgca
     1741 tttgtttcgt gtcctgctcg gaagacacgc tctttaattt catttgtgat atgtggaatg
     1801 acttgaaccg tgccaccaag gtaatctcca cgacgctctt tacgaataac agaagaatac
     1861 actttaccag ttgtcacgtt gctatactgg tttaagttaa tgtcgataaa cgactcatag
     1921 tgaccaaggt caaggtccgt ttcagcacca tcactcgtaa cgaatacttc cccgtgctga
     1981 taaggactca tggttcctgg gtccacattt aaatatgggt caaatttttg gatggtaacc
     2041 tttaacccgc gatttttaag tagtcttcct aaggatgctg ctgtgattcc ttttccaaga
     2101 gaggatacaa ccccacctgt tacaaaaata tattttgtca tgactattcc cctactttcg
     2161 tctcgtaatt gtattttgtt agtgtatcca agggatagaa aaaataaaaa acgactccct
     2221 acaaaaagtg tagggagcgt tcagatttat atccgtcctc ttttaaagag cccaaataag
     2281 attctacata cgtgataacg tgaagtcaag aaaaagaaaa attaaaattc ttcttcctca
     2341 tcttcctctt cctcttcctc ttcctcgtca tcctctacga tttctaaatc atcgtcatcc
     2401 tcgtataatt tcttcatcgt ctgtgtcgtc gaaatcttct aagtcttcat cttcagcaag
     2461 atcttcatct tcttcaaaat catctagttc gtcaagatcc agttcttcct catcgatatc
     2521 atcgaataga tcttcatcat cctctgattt tttctttttc ttgcgaactt tgatcgttgg
     2581 agctgtctct tcttcgattt gatctactgg gtaccattca cgaagacccc atttgttatc
     2641 accgattgca aggaaacggc catcgatgtt catatctgtg tagaattgtg caatttttcg
     2701 cttcatttct tgatctgaaa tttctaggat tttttgaata tcttttacga tttcattaaa
     2761 cggaattgcc tcttttttct ctagtaaaat ggcattcgtt atttcgataa aagacacttc
     2821 ttttaattcg tcttgtgata attctcttat gttcaccctt cgcacttcct ttctctattt
     2881 cacactctcc acgagtttaa gcgcataaaa tttccatttt aattagaaac atatacatca
     2941 ttataaacaa tctacaatac tttatgctag atatatctat gtttcttagc tttttttgtt
     3001 ttattcttta ttctttcggt actgtcgtat tgctagatat aaaaagtaaa aggaaagcag
     3061 aaagaaggta atagcgccta attgcttttg atatagaaaa attccgataa aaacaacgat
     3121 gataataatt aatcctttta tataaaacat aggttcactt cctagacacg aatggcttgc
     3181 cacccctaag tataacgaat tttcgaaaat tgttgtatta acctagtgta aaatatttct
     3241 ttttttatgt tgaggatgaa acatgagttt gtttgattga attgaatggc cattcctacc
     3301 attcctctag tacctgatac ccttcaccat ctgaattttt ttatcactat tcaccacaat
     3361 taaacaaaaa aacggggtgg ccaatctttc cccgcttaag tatgccctac atatttcttc
     3421 tatattgacc gccgacttcg tataaagctt gtgtgatttg tccaaggctc gctactttga
     3481 cacattccat gagttcagcg aagatatttc cgccactcat ggctgtttcc tgtagatttc
     3541 ttaaggctac cctcgtcttg tcttcatgct cctgctggaa ttctcgaaga tgacgtattt
     3601 gagcatcctt ttcttctttg gaggcacgtg ctagctccat attatccatt tcttcctcag
     3661 aaggagggtt tggatttaga tacgtattca ccccaataat cggtagttca cctgagtgct
     3721 tcttcatctc ataatgcatg gattcttctt gaatcttccc tcgctgatac tgcgtttcca
     3781 tcgcgccgag taccccgccg cggtcattaa ttctttcaaa ttcttgcagt acagcttcct
     3841 ctactaaatc cgttagttct tcaattataa acgagccctg aagagggttt tcattcttcg
     3901 ttaagccatg ttcctttgtg ataatcattt gaatggccat agctcgtcga acggattcct
     3961 ctgttggtgt tgtaatggcc tcatcatatg cattcgtgtg gagagaattg cagttatcat
     4021 gaagagccat aagtgcttgt agagtggtac gaatatcatt aaagtcgatc tcttgtgcat
     4081 ggagacttcg acctgatgtt tggatatggt atttcagttt ttgacttctt tcgttcgcac
     4141 catatttgtc tttcatcaca atcgcccaaa ttctgcgcgc cacgcgtcct ataaccgtat
     4201 actctggatc tagcccattg gagaagaaga aggatagatt tggcgcaaag ctatcaatat
     4261 ccatgcctct acttaaataa tactccacat acgtaaatcc attagcaagg gtaaacgcca
     4321 attgagatat tgggttcgca cccgcttctg caatatggta gccggaaata gaaacagaat
     4381 aatagttacg cacctcgtgg tcgataaaat attgctgaat atctcccatc atgcgaagcg
     4441 caaattctgt tgagaatata cacgtgttct gtccttggtc ttcttttaaa atgtctgctt
     4501 gaacggttcc tcgaacaacc tttagcgtgg cttccttcgt ttccataaat tcttcaagcg
     4561 ttaacggtcg gccgacttct tcttcacgct tattcacctg ctggtcaatt gccgtgttca
     4621 taaacatcgc aagaataatc ggtgcaggtc cattaattgt catagatact gacgttgacg
     4681 gtgcacataa gtcaaagcca tcataaagct ttttcatatc ctgaagagta caaatgctca
     4741 caccgctctc tccgactttt ccgtagatgt cgggacggtg tgctggatct tctccgtata
     4801 aagtcacgga atcaaatgcc gtactcagtc gcttcgcatc gtcatccttt gataagtaat
     4861 ggaagcggcg gtttgttctc tctggagtcc cttctcctgc gaactgacgc ttcgggtcct
     4921 ccccttttcg tttaaaagga aacacacccg ctgtataagg gaaagcacct ggtacgtttt
     4981 ctttatacac ccattttata atttcaccgt agtccttgta tttcggtagc acaaccttcg
     5041 gaatagaaag cccagataaa ctttttgtct ttaactcggt gacgatttct ttccctcgaa
     5101 tgtttgtaac gaactgatcc tgttcatata atctctttgt ctcttcccat ttggaaagaa
     5161 tctctttact ttcagcattc aaggattctt gtacgctttc ttttacacgg ttgaaagatt
     5221 cagggacttc ttgtaaaagg tcttcggtac cctgcagctg gaatagttgt cgcgctacct
     5281 tctcttgctc ggctacctta cggtgatacc ctcttaccgc ttctgaaata tctcgcagat
     5341 agtagcgacg attgggcgga atgatcacat tctgcttctc cactacttcg tgtttactaa
     5401 acgcagtcgt ccatcctgaa cgaaaacgct cattcatctt ctctgtaatg gctgcgaaca
     5461 aggcgtttgt ccccggatca ttaaactgac tcgcgatggt tccgtagaca ggcatctcag
     5521 aaagctcctg atcaaacagc atacgactgc gctgatattg cttctgtacc tgacttctcg
     5581 cgtcttctga ccctttccgg tcaaatttat tgatcacaat aaaatccgca aagtcaatca
     5641 tatcaatctt ttctagctga ctcggtgccc cgaattcact cgtcatcacg tacattgaga
     5701 ggtccgcaat ctccgtgatt tctgcgtcgc cttgcccaat cccgcttgtt tcaacaataa
     5761 tccaatcgaa tccggccgct tttacgatac tcacagcatc acgaattgca agagataact
     5821 ctgacttaga gccacgagtg gccaagcttc gcatatacac acgtgaatgg aagatggcat
     5881 tcatacgaat tcggtctcca agtagggcac ccccggtctt ctgttttgtc gggtcaatgg
     5941 atagtatggc cactcgcttt tcaggcactt catgtaaaaa gcgtcggatt aactcgtcgg
     6001 ttaacgagct tttacctgcg cctcctgtcc ccgtgatccc aagaactgga acatccttct
     6061 cgtgtttctt cacagatgtc agaatgctct cggccgttgc tgccacgtca gtagcctccc
     6121 ttacatgaag ctctgcaagg gtaatcagct tactaacagc attcacattg ttcggagaaa
     6181 cgtcgtttac ctgctcggca atctctcccc caactgtcag gaaatcacat tccttcaaca
     6241 ttcgattgat cattccctgt aagccctgtg ttctcccgtc ttcaggtgag aatatgcgcg
     6301 caattccgta ctcatgaagt tcttttatct cacgagggat aatcaccccg ccgcctccac
     6361 cgtaaattcg gatatgccca gcgcctcttt cctgcaggag atcatacata tatttaaagt
     6421 attcaacgtg cccaccttgg tacgaagata tcgctatccc ttgaacatcc tcttgaattg
     6481 ccgcattcac tacttcttca acagaacgat tgtgacctaa atgaatcact tccgccccgc
     6541 tgctttgtaa aatacgtcgc ataatattaa ttgacgcatc gtgaccatca aacaaactac
     6601 ttgctgttac aaacctgata ctatgctttg gttgatagat ttccatgctt aaccttcccc
     6661 cttttacact caataccgtg acaaacaagc tctgtttgaa gctcaatata ctcctctatg
     6721 gtgtaccgcc gctgaaaggc ccaacgtcta aatgcccaca tctgcccttg aacgaagata
     6781 tcgttcgctg ttacatagat ttgttgttca tcaagctcaa tttctcccga tctcacgcag
     6841 ttgcgaagga gtacctgaaa catgtcgacc atttccattt ctttttggag cacgtagggg
     6901 atggcgtcct tcgataagga ctttgcttcc tggtacataa ctagtacctc ctcctgaagc
     6961 tcatccatta gatgaaaata gtacgctatt gcttgcttta gctgcgaaat ggttcccccg
     7021 ttcacatcca tttcggacag cttctccttc actccgtcgt aaatattgtc acaaactaaa
     7081 tagaggacat cttcctttgt cctgatatat tcataaagcg tcccaatact aaaccccgca
     7141 gcctttgcaa tctcccgcgt tgtcgttcgg tgaaacccct tttctttaaa cagcgtcacc
     7201 gctcccctaa tgatctggtt ccgcctaatt tcaattaatt tttcatcttt aactgatgcc
     7261 ttaacaggtc ttttcataca cttccactcc cctaagtgga atttggtttt tctcctattt
     7321 cctttgttcg taaggtgcgc ggaggttaaa ggattggatg agatttttgg cgggttggcc
     7381 gcttttggtg ggttgggtgg catgcctttg gtgagttggt ccttttttgc gtgcgtctac
     7441 aagctctttg cgtgcacttt tcaccctttt gcgtgcactt ttccgccgct tgcgtgcaga
     7501 tttcggactt ttgcgtgcac ctgctccttt tccgtgcggc tacaggctct ttgcgtgcac
     7561 ttttctgcca cttgcgtgcg gatttccgcc acttgcgtgc aggtttaagc cttttgcgtg
     7621 cacccgctct ttttccgcgg ctgctattta acacgcccac accaacttcc catccattcc
     7681 acccactacc tcaaacttcc tcccaatcct cccctaccaa atagtcttac ttcgtcaaca
     7741 tcctagaaat aactaaacgt tggatttcct gcgttccttc gtagatttga gtaatctttg
     7801 cgtcacgcat aaagcgttcg actggataat cttttgtgta accatagcca ccaaagattt
     7861 ggacggcatc cgttgtgacg ttcatggctg tgtctcctgc catgagcttc gacatcgcgg
     7921 actctttgcc gtatggcagt ccttcagatt ctaaccaagc tgcttgatag gttaatagtc
     7981 ttgacgcttc gatgcttgtt gccatatcgg cgattttaaa cgaaatgcct tgctggctcg
     8041 caatcggctt cccgaattgt tcccgttctt ttgcatagcg aatactttca tccagtgcac
     8101 cttgggcgat tcccactgct tgcgcagcga ttccgtttcg tccgccgtct agcgtcatca
     8161 tggcgatttt gaatccttct ccaaggtttc ctagcatatt ttcttttggt actttacact
     8221 cttcaaaaat aatttccgtg gttggagagg aacgaatgcc tagttttttc tctttctttc
     8281 caaccgagaa gccttcaaat ccgctctcta cgataaaagc agttgtgcca ccacgttgtt
     8341 tcttttctga atctgttaac gcaaatacta catagatgtc tgcaatgcca ccgtttgtga
     8401 taaagatttt acttccattt aaaatataat gatctccctc gaggcgagcc gttgttcgca
     8461 ttccccctgc atcagaacca gagcctggct ccgttaaccc gtaagcaccg attttctctc
     8521 cttgtgccat tggacgaagg tatttttgct tttgctcttc tgtaccaaat ttatagactg
     8581 gccagcctgc aagtgacgta tgggcagaca gcgtaacccc agtagaggca cacacgcgtg
     8641 atagctcctc cactgcgata cagtaggcta agtagtcgct gccaattccg ccgtactcct
     8701 ctggccacgg aattcctgtt aagcctaact ctgccatttt atcgaaaatg tctcggtcga
     8761 agcgttcttc ttcatctcgc tctgcagcgg ttggcgctac ttcgttgacc gcaaaatcgc
     8821 ggaccatttt acgaatcatt tcatgctctt cacttaattt gaaattcatg tcccatccct
     8881 ctttttccct ctattttaat aaatgctttc ctaatacgac gcgttgaatt tcgcttgtgc
     8941 cttcgtagat ttcggttacc ttcgcatcgc ggaagtagcg ttctactgga tagtcttctg
     9001 tgtagccata gcctccgaat acttgtatgg cttctgttgt gacatgaacg gctgttttag
     9061 acgcaaacag cttcgccatg gaggcttctt ttccacatgg gcgcccttct gtacggaggt
     9121 ttgctccttg atagactagt agcttcgatg cttcaatagc agttcccata tcggctaact
     9181 tgaaggctag gccttgttgg gcagcgattg gctttccgaa ctgaacgcgc tccttcgcat
     9241 actcaaccgc ctcatcaaat gctgcttctg ctatccctag agcttgggca gcgattccga
     9301 ttcttcctac atcaaggttt gccatcgcta ttttaaaccc ttccccttcc gcacctaaaa
     9361 gattggaagc tggaaccaac atatcctcaa aggttaattg aacggtgcga gagccgtgaa
     9421 gacccatttt ttcttcgtct ttcccaatga caaggccagg cgtatccttt tccacgataa
     9481 acgctgaaat gccgcgtgtt cccttttcag ggtccgttga agcaaagacc acatacgtat
     9541 cagcttctcc gccgttagtg ataaagattt tactaccatt cagtcggtac tgatctcctt
     9601 ctagaacggc acgagttcgt agacttcctg cgtcagatcc tgagcttggt tccgttaaac
     9661 agaacgcccc taagtactgt ccactcgcaa gctttctcac gtacttttgc ttttgctcct
     9721 ctgaaccgaa ataaaggatc gggttggtgc caaccgatgt atgaaccgat aggacgacac
     9781 caagggttgc actgaccttt gatagctcgt gaattgcgat aatataggac gtgaagtcca
     9841 ttcctgcccc gccgtattct tctggaatgg tcattcccat taagccaagc tccgccattt
     9901 tcgataaaat agggcgtgga aactctcctg cttccatcct ttctacaaac ggtgctactt
     9961 cttcgcgtgc gaagtctcgc accattttac ggaccatttc ttgctcttca gtgaataaaa
    10021 agttcatccc ctaatccccc ttagctgtat tcgtaaaaac cacggcccgt tttcttcccc
    10081 aaccagcctg ccttcacata ttttctaagt agaggacatg ggcggtactt atcgtcacca
    10141 aagccctcgt gtagcgtttc cataatatag agacatgtat caagcccaat aaaatccgca
    10201 agcgtaagtg gtcccatcgg atgattcatc ccaagcttca tgacctcatc aatcgcttct
    10261 tttgatgcca ccccttcgta taaggtgtaa atcgcctcgt taatcatggg cattagcaca
    10321 cgatttgaca cgaacccagg gaagtcattc acttcaactg gcacttttga aagagactta
    10381 ctcatatctt caacggcttg atacacctca tctgccgtcg ctaatccacg aataatctca
    10441 actagcttca tcactggcac gggattcata aaatgcatcc caattacctt ttcaggacgc
    10501 tttgtcacag ccgcaatctc cgtgatgggg agtgaagagg tgttggtcgc taaaatcgtg
    10561 tgctctggcg caatgctatc aagctttgaa aatatatctt ttttaatagt catattttct
    10621 acagctgctt caatgacgat atctacgttt ttcgcgtcct caagatttgt tgataacgtg
    10681 agacgggaga gaaccccttc tttctcctcc tctgtcattt ttcctttatc cactgagcgc
    10741 gctatgttct ttgtaatact tgctaaccct ttttccgcaa actcttgttt aacgtcgttt
    10801 aaaacgacgt catatccccc tcgagcacaa acctgggcta tccccgagcc catttgtcct
    10861 gctcctatga ccattacacg tttgattgcc ataccttctc cccctttgct tttctcttac
    10921 gcttttggaa cctcaatcat aatggcatcc ccttgaccgg taccactaca aatcgctgcc
    10981 actcctattc ctccacctcg acgcttcagt tctagcgcga gagttaagac aattctcgcc
    11041 ccacttgctc cgatgggatg accaagtgct actgctccgc cgtttacgtt cactttgtca
    11101 tgatccaact ctgcaattct cccgctcgtt aacgccacag ctgcaaaggc ttcatttatc
    11161 tcaaacaggt caatgtcgtc taaggacttt cctgtttttt gtaaaagttt attgatcact
    11221 aaaccaggcg tttctggaaa ctcttctgct ggcaccccaa cctctgcatg agctaaaatg
    11281 gtcgcaagcg gtgtctttcc taactcttct gccttttcat cactcattaa aacaagggca
    11341 cacgctccgt cgttcactcc aggtgcgttc cctgccgtaa tggtgccatc gtgatcgaac
    11401 actgggcgaa gggctgatag cttttcatac gacgtatctt ttcgaggagc ttcatcatga
    11461 tccacgacca ccggctcgcc tcttcgctgt ggcacttcga ctgaaacaat ttcctcagca
    11521 aatgttccgt tgtccatggc gatgacagca cgctcatggc ttctgaagga ccagcgatcc
    11581 tgctcttctc gtgtgatttc aaatttggtt gctgttctgt ttccgtatgt ccccatgtgc
    11641 acatcgttaa agcaacacat taatccatcg tgaagcatca aatcctttac ttgatgatcg
    11701 cccatcttta gaccccatct tgctttcgga atagcatacg gagcatttga catactctcc
    11761 attccaccgg ctacaatgat gctctcatct ccagcacgaa tgatctggtc tcctagagat
    11821 atacttcgca tgcccgaagc acatacttta ttaatggttt ctgtctttac ttcccacgga
    11881 atacctgcat gtcttgctgc ttggcgagaa ggtatttggc cttgaccact ttgtaacaca
    11941 ttccccatta ttacttcgtc aatttgatct tttgcaaccc ctgctctttc gagtgctgcg
    12001 ctaattgctt taccccctaa ttcggatgca gtaaagctac taagagctcc tccaagcttc
    12061 ccaaatggag ttcttgcccc cgcaataatc acagtttttg tcatactgtt tctcccctct
    12121 cttttgttcg taaacgcttt caaaaatgac gctgactgaa cgctcgctca tttttgtagc
    12181 aaataaaaaa gaaaaacctt cccacctcca ttataaggaa agttcttggg tttttacaga
    12241 attttaagaa attattttat aatactattt cactctttga atcacacgtt gtggaatcct
    12301 attttctttt tacacttttt tctaatttag gattgactta ttcagttgaa ctgtataaaa
    12361 ttcaactata tagttcaact gtataaagga gattaatatg aaaagaacta agcatttacc
    12421 attaaccgag acggtgttct acgttttatt gtccctactt gaaccaagtc atggctatct
    12481 tattcttcag aaagtagaag agttaagcaa aggcagcgta cggatggcag ctggcacgct
    12541 ttatggtgct attgagaatt tagtaaagtt agggttcatc gagccggttg aatcaggaga
    12601 caagagaaga aaggtgtatg ttattaccca tttaggttta gaggttttac aagcagatca
    12661 acaaagaatg tcacacatgg ttttattggc agagaacgca ttaaaacaat aggagtgatc
    12721 atgatgaaac ggttacgtta ttttatggat ttagataaag aagaagagtg gttgaatgaa
    12781 aaggcaaaag aaggcaatgt actctatcaa aaatcattct tctatcactt taaggccacg
    12841 acggagaaag atcccacttt gaaactcgat taccgaacgt tcaagcatag agaagatttc
    12901 gaagactata tagccctttt tgaagattct ggttggaagc atatagcagg gagtaaacgt
    12961 tctggagtcc aatattttca gcgtgtttct aacgaggcaa caggggatat tttctccgac
    13021 tctcattcaa aggctgaacg ctataagagg tatgcgcaaa cgtccgcaag tttactattc
    13081 acatatttta ttttattaat tgggttaatg agcgcagacg tccttcatct gcaagaaatg
    13141 ctccagccaa agtcattgta ttatacacct ggattgtggg agaaggaggg tgctgatttt
    13201 tggagagcat ttttatttga aacgccattc gcatttttca gaggtttcgc ttggttgctc
    13261 ttccctattg ccattgtgat ctttttcttg tccgctacga tttcttatat aaaatatgaa
    13321 aaattagtaa atcagtagta atataatttt ttagtatgtt tacgatgagc ctctccctaa
    13381 aataatcctc ttccagttaa atggatgata atattatgaa gatgggtgat tatttgaata
    13441 cgattgcctg gttcattgtt acctgtgaat ttagtttctg gattgtcata ttgctcggat
    13501 tatatattcg gtattctttg ggtaagaaat cgctcgaatt aatcttttta gccttaacat
    13561 ctttaattga tttggcttta aatgttttga ccacgttgga tttagcaagg ggctcgactt
    13621 ctacggtcgc tcaatgttaa ttatttggct atctaaacat atttaactcc ttggaaaatg
    13681 tgtgttcttt acctttattt aatgatggat taggttttaa tcataagggg ggagctcttc
    13741 ttgcttcctc tccctctttt ctgcaccgcg ccaggctcgc gcggcgctct tcttgcttcc
    13801 tctccccctt ttctgcaccg cgctcggctc gcgcggcgct cttcttgctt ccttctcctc
    13861 ttttctgcac cgcgccaggc tcgcgcggcg ttcttctccc ttccttctcc tcttttctgc
    13921 actccctctt
//
