LOCUS       NZ_KI260397.1          15255 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_KI260397.1 Ruminococcus callidus ATCC 27760 Scaffold283, whole
            genome shotgun sequence.
ACCESSION   NZ_KI260397
VERSION     NZ_KI260397.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 00:42:46
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15255
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15255
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[176:5255]"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      177..5255
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
     cand_cluster    1..15255
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /kind="single"
                     /product="Rnf_complex"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15255
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Rnf_complex"
                     /region_number="1"
                     /rules="(Complex1_51K and minimum(5, [FMN_bind, FeS, Fer4,
                     Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N, SLBB]))"
                     /tool="gutsmash"
     CDS             complement(177..1025)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FeS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4_9"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1020:4Fe-4S binding domain (Score: 29.6; E-value:
                     1.4e-09)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_1"
                     /sec_met_domain="Fer4_7 (E-value: 3.7e-09, bitscore: 27.6,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_9 (E-value: 2.8e-10, bitscore: 30.8,
                     seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 6.1e-08, bitscore: 22.8,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="FeS (E-value: 7.8e-16, bitscore: 48.0,
                     seeds: 364, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.3e-06, bitscore: 16.6,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFVYPILIFVVMGIVFGVLLVVISKVFAVKTDERAVQITEALPG
                     ANCGACGYAGCADYADAIVNKGAPMNACLPGGANAAAAIGAIMGVSVTASERMVPVLHC
                     NGTCEATNRKFTFDGVQSCTAAKRFYGGTGVCAYGCLGLGDCVSVCENDVISIKDGIAT
                     FCTEKCVACNKCAKVCPNGLIELRSEKKKVDVRCSSRNMGKVAMQSCQNSCIGCKKCEK
                     VCKFEAIIVENAYPVIDYDKCKSCGLCAKECPRGCIENLRKPKVVAPKPEPVKEKPAAE
                     E"
     CDS             complement(1038..1622)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_2"
                     /sec_met_domain="Rnf-Nqr (E-value: 4.8e-60, bitscore:
                     192.9, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTYIAIIIAAVLTNNFVLSKFYGCCPFLGVSKKLDSSVGMGAAV
                     VFVMLCASLCTYPIYYFVLKPLELTYLRTVAFILVIAVFVQLLEMVIKKLMPPLYKTLG
                     VYLPLITTNCAVLGVTIMNIDESYSFGQSLVNAVFSGVGFALAMVIFSGVRSRVDDAES
                     ETPKMFRGVPATLIAAAIVSMSFLGFGGLGQ"
     CDS             complement(1619..2284)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_3"
                     /sec_met_domain="Rnf-Nqr (E-value: 4.3e-69, bitscore:
                     222.4, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYGKEFTKGILKENPTLVGLLGMCPTLAVTTAAFNGIGMGLSTM
                     FVLICSNIVISLLRKVIPEQVKLPCYIVIIASFVTVIQFLVHGFVPALYTSLGAFLELI
                     TVNCIILGRAEMFASKNNVLASALDGAGMGLGFTLALFLMGSVREILGSGTWFGLTIPG
                     MVGHTMSLFVMPAGGFLVLGFVIAVVCALSKKNPPKKIGCASCPNAAACHAKCEEADA"
     CDS             complement(2281..2850)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FMN_bind"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_4"
                     /sec_met_domain="FMN_bind (E-value: 2.9e-18, bitscore:
                     56.8, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEAKTSSVKSMVLPPLVLAIICAVCCAFLALANMVTKDKIAAAE
                     ADAIQTSLKQLPDAGTFEEVTDFQPENNEKASATGLYLDENGQYAVLVTADGYNKGGLQ
                     VVIGVDKGGAVTGVSFVTVSETPGLGTKVQSNPELLVDHLVGLSDSDAVDGVDNITGAT
                     YSSKGMKAAVNCALATVHANQEVTGA"
     CDS             complement(2852..3847)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: NQR2_RnfD_RnfE"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_5"
                     /sec_met_domain="NQR2_RnfD_RnfE (E-value: 9.6e-113,
                     bitscore: 366.7, seeds: 472, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLLTVSPSPHVRSGMTTQKIMLLVVLALVPAIVAATVVFGPRA
                     LFLVVFCAVISMFTEMVCQKLMQKETTYTDGSAMLTGVLLALNLPATLPLWEAAIGCVF
                     AIAVVKQLFGGLGCNFANPAITGRVFLLLSFGGDMTTWVKPFFYRGRSFFEMFAATGNL
                     PDGVTGATPLASGDAGLLDLFLGNVGGSLGETSVLALLIGGVFLLVLGLISWHTPVAYL
                     GGLAVLELFYTLATGGEMTDVLYALLSGGVILGAFFMATDYVTTPMTGAGKLIFGLGCA
                     VLTFVIREFANMPEGCSFSILLMNLLTPYIDKLTMTHPFGAKPAVKKEAK"
     CDS             3901..4128
                     /locus_tag="ctg96_6"
                     /transl_table=11
                     /translation="MCSCNMRYRSFQKDLTKQYILNITHRVKDFSKTPPPPNGGPPRTE
                     RSPLPCGHLPTPWGVTPFRGGLFSCHYYNE"
     CDS             complement(4242..5255)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: SLBB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_10"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Complex1_51K"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_7"
                     /sec_met_domain="Fer4_10 (E-value: 1.2e-10, bitscore: 31.9,
                     seeds: 59, tool: rule-based-clusters)"
                     /sec_met_domain="SLBB (E-value: 1.9e-12, bitscore: 37.4,
                     seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Complex1_51K (E-value: 4.2e-49, bitscore:
                     156.8, seeds: 88, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.3e-06, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEIETDGEQTVSPDVRPPAVTDKESFLKAVRESGLVGMGGASFP
                     THVKLNPKQTVDTLVINAAECEPYITSDYRQMIEAPDEVLDGVRQVLHWLQIPKAVIGI
                     ETNKPEAIRVLTEKTKSEPEIQIFSLPTTYPQGAEKVLIYHTLGRTVMEGQLPADQGVI
                     VMNVSSVAFLSRYLKTGMPMVQRMVTVDGDAVGKPCNVMVPMGTPVQDVLDFAQCDMER
                     AKKVLYGGPMMGIAVPDTADPILKANNAVLALEKFVSPKPTACIHCGRCVHACPLRLMP
                     TEIAKAFRQEDSEALGKLKVTLCMNCGCCTYACPAKRPVAETNQLAKAFYMSSIKK"
     CDS             complement(5877..6836)
                     /locus_tag="ctg96_8"
                     /transl_table=11
                     /translation="MMHGKKLFAVLLPLVLTACTSAKPAQTETVWAMDTACTISLHGSG
                     VSETASLLKTLDKTLDNYQENSAISRLNQSGTISGNDTIYQLVSETAALQKRFGDSVDL
                     TVGQLTALWGITTDTPHVPTAAELEPVLKTISPDHVTAADGTVTLTDGAQLDCGAVGKG
                     YSLDCVKEALDQSGTASYGTVSMTSSILCYGEKPDGKPFQIEIRDPSGDGTLGTVSTDS
                     CFLSTSGGYERYFIADDGKQYCHILDPKTGMPAESDLTTVTVFCDSGLQSDFLSTLIWL
                     EGTEHLEEHLHAEDYQIVAQDQSGKLYVSDGLHFTPNA"
     CDS             7020..7682
                     /locus_tag="ctg96_9"
                     /transl_table=11
                     /translation="MYRLSNEFHNGGLFFMKGYRISVIRHGRTAANDKGIYIGRTDFPL
                     SDKGAAELAGKMDEFRYPNVARVYSSPLRRCTETAEILFPDVQIQTVDNLIEMDFGTFE
                     GKSADELVHKPEFKEWLKGGMDCRPPQGESVKEVQLRIFRSLREIIRDMMQEDLLHCAV
                     ITHGGMIANMLAGFGLPKISPQELQCQPGEGFDIYVTADLWQRSQAFEILGMVPYSR"
     CDS             complement(7638..7880)
                     /locus_tag="ctg96_10"
                     /transl_table=11
                     /translation="MCSVRNRFHMHTKTFFHSKNSCGIAYKTPNLPQFVKSKSKTALFS
                     GKLRQRVRFSTIIFPTIIKVSSSGIRHHAQNLKRL"
     CDS             complement(7882..8232)
                     /locus_tag="ctg96_11"
                     /transl_table=11
                     /translation="VSPLLCRRFAILELGAFQMQVGDLFQQVFLLCRCQSGIIPEQVLL
                     TVGSQISQCFFIRHVYNPFLKNRCRTGSIVFGNLGNLGNPAEIPVSIGINTLYHNMQEN
                     TSLFEKKQAFSK"
     CDS             8389..9282
                     /locus_tag="ctg96_12"
                     /transl_table=11
                     /translation="MYNMGKTRDLYIFECLIHNLKKVTDEAEAYVPLMIMTSEQNDAET
                     RAFLKEHDYFGYSADNVHFFKQETEPAVDENGKIFLNAPDQIVLSPNGNGGWFSSMQRA
                     GLLQTIEEIGLEWLNVFAVDNVLQQIADPVFIGATIASGCASGGKVVSKADPDERVGVL
                     CLEDNKPSIVEYYEMTEEMRTLRNEDGSLAYRFGVILNYLFRVDALMHTLGSDFPLHIA
                     HKKISHLNEAGELVKPEEPNGYKFETLVLDMVHMQDSCLAFEVDRSKEFAPVKNAVGVD
                     SVETARALLEQNGVVL"
     CDS             9712..11265
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_8"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg96_13"
                     /sec_met_domain="NAD_binding_8 (E-value: 1.5e-14, bitscore:
                     44.5, seeds: 167, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKVVILGGGPAGLTAAYELLKDTKDEYEVTILEESGVLGGISQT
                     VRYHGNRMDIGGHRFFSKDDTVMKWWSDLMPIQGQPSFDDKKLGREKPLEAGGPDPEKE
                     DVVMLVRDRVSRIYFNKHFFDYPVTMNKNTIQSMGFATTMKAGFSYLGSCISKKPETNL
                     ENFYINRFGKVLYSMFFEGYTEKLWGRHPSEISADWGAQRVKGLSIRAVLKDMISKRSG
                     KKNNKNAETSLIEQFWYPKYGPGQLWELVGKKAEEKGCKILFHHSVKTIHTENGKVTSV
                     VCETPEGSKTISGDIFISSMPIQDLIAGMDGCQNTEVQRIAAGLPYRDFVTVGLLVNKL
                     NLVNQTGTKTLGNIVPDCWIYVQDKGVKLGRIQIFNNWSPYMVEKPEDTVWIGLEYFCA
                     EGDKFWNMSDKECVDFAVKELTKMGVISEGAVLDAHREKVKKAYPAYFDTYAQFDQVVD
                     YLNTFENLFCVGRNGQHRYNNMDHSMLTAIHAAEAIKHDSTDKGPVWNVNQEKEYHEQK
                     "
     CDS             11252..13051
                     /locus_tag="ctg96_14"
                     /transl_table=11
                     /translation="MSRSKLFSKNSKRTGNRSHGCRCSCICLPAAESNRYFHLLERHPY
                     LCSLFVCLLLNALYFGGEAYLAQNAVLLETLALLAAGAAVLWRLVRYQPQKKPAAVCVS
                     VVYTLLVLAGAWCYHEARHSGLWVLVGGCAVLSGLFWLTRKQYHNQRRRVSLLLLGTSF
                     LLKFFYVCYTPITRRQHDVGKFGDADNHAGYITYLLQHHQLPDFDPRGHWQFYHPPLHH
                     MISALWLWLSEHVFGISNAAAQESLQTLSLFYAMAIVITAYRILRHFRLEGMALYVPLA
                     VVAFHPGFILFSGSINNDALSVVFILGAVLWTLKWYEKQTWEGIVKIALCIGLGMMTKL
                     SAAMVAIPVAAVFLVVLVRQLRQRNWRILGQFGGFAVICFPLGLWYPVRNLVRFGVPLT
                     YVQEMPENSVQYLGEQSFFSRILDFSPHQVASVFEQWVQRTGGSYNEYNPLIALLKNAM
                     FGEYINEYTLDCSLWRILTGVVLFWLNVVLAAAAFAAMLWLCGKREQTGGRLPKLFLVL
                     FYAVLMGGFYQLSAAEPFTCSMNYRYITPTCVIGAVFLGLAFQRFRNGKKPVCRWLCGI
                     GWTLCGCFAACAAAFYIGLGLIG"
     CDS             13825..14493
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Hexapep_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg96_15"
                     /sec_met_domain="Hexapep_2 (E-value: 3.5e-13, bitscore:
                     39.6, seeds: 55, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKLATQYALTERRNLIMTEEEKMLAGKLYDPSDPKLDARRLLAH
                     RLSMEYNNTLETDAEKRKAILAQLLPDCGEDTFLQGPLQFDYGCYTRFGKRCYANFNLV
                     VLDVCPVTFGDDVFIGPNCSFVSPMHALLPEERNLKQRPDGSYYDLEYGKPITVGSNCW
                     FGSNVTVCGGVTIGEGCVIGAGSVVTKDIPPHSLAVGNPCRVLREITEKDSVQYKGITA
                     "
     CDS             14561..14878
                     /locus_tag="ctg96_16"
                     /transl_table=11
                     /translation="MAVIRLTKENFQAEVMQADKPVLIDFYADWCGPCQMVSPIVDEIA
                     GERSDIKVCKVNVDEQMELAQSFGVSSIPTLVVISGGKLVKHAVGAQPKSAILDMLEAV
                     E"
ORIGIN
        1 gaatctttgt ctaacttttt ggggtcagtt cattgcggcg gttatttctt ttaatcgttt
       61 tccgttaatg tgggagcaac cgtctgccgg tatgctcttt ctatttttat tatacgcatt
      121 tttctccggt ttgtcaagag gcagaccggc gttttttcgg ttttgcggta ttgcctttat
      181 tcttccgccg ccggcttttc ctttaccggc tccggcttcg gggcaactac cttgggcttc
      241 cgcaggtttt caatacaccc tctggggcat tcctttgcac acaagccgca ggacttacac
      301 ttatcataat cgatgaccgg ataggcgttc tcgacgatga tcgcctcaaa cttgcagacc
      361 ttctcgcact tcttacagcc gatacaggag ttctgacagg actgcattgc caccttgccc
      421 atattgcggg aggagcagcg aacgtccacc tttttctttt ccgaccgcag ttcgatcagc
      481 ccgttgggac agactttcgc acacttgttg caggcaacac acttttccgt acagaatgtg
      541 gcaatgccgt ctttgatcga gatgacatcg ttttcacaga cagaaacgca gtcccccagt
      601 cccagacagc cataggcaca gacaccggtg ccgccgtaaa accgtttcgc agcggtacag
      661 gactgcacgc cgtcaaacgt gaacttccgg ttggtggctt cgcaggtacc gttacagtgc
      721 agcaccggca ccatgcgttc cgacgcagtc acagacacgc ccatgatggc accgatcgct
      781 gccgcagcat tggcaccgcc gggcagacag gcattcatgg gagcaccttt attcacaatg
      841 gcatcggcat agtctgcaca gccggcatag ccgcaggccc cgcagtttgc acccggcagt
      901 gcctcggtga tctgcaccgc acgctcatcg gtcttcaccg cgaacacctt tgagatcacg
      961 accagcagga caccgaacac aatgcccatg accacaaata tcagaatggg atagacaaat
     1021 tccatgacac accctcctta ctgtcccagt ccgccgaagc ccagaaaact catggacaca
     1081 atggcggctg cgataagcgt tgccggaacg ccgcgaaaca tcttcggggt ttcgctctcg
     1141 gcatcgtcca cgcgggaacg gacaccggag aagatcacca ttgccagtgc aaagcccacg
     1201 ccggagaaca cggcattcac cagcgactgt ccgaagctgt agctttcgtc gatgttcata
     1261 atggtaacac ccagcacggc acagttggtg gtgatcaaag gcagatacac gcccagcgtt
     1321 ttatacagcg gcggcatcag ttttttgatg accatttcca gcagctgtac aaatacggca
     1381 atgaccagaa tgaatgccac tgtccgcaga taagtcagtt ccagcggctt caggacaaag
     1441 tagtaaatgg gataagtgca cagggacgca cacagcatga cgaaaaccac tgcggcaccc
     1501 atgcccacgc tggaatccag ctttttcgaa acgcccagaa acggacagca gccgtaaaat
     1561 ttcgacagaa cgaagttgtt cgtcagcaca gccgcaatga tgattgcaat ataggtactc
     1621 atgcgtcagc ctcctcacac tttgcgtgac atgccgccgc attggggcag cttgcacagc
     1681 cgatcttttt cgggggattc tttttggaca gggcacagac cacagcgatc acgaagccca
     1741 gcaccagaaa gccgcctgcc ggcatgacaa acaggctcat ggtatgtcct accatgccgg
     1801 ggatcgtcag tccgaaccat gtgccgctgc cgaggatctc ccgcacgctg cccatgagga
     1861 acagagccag tgtgaagccc agtcccatgc cggcaccgtc cagagccgaa gccagcacgt
     1921 tattcttgga ggcgaacatt tccgcccgtc ccagaatgat acagtttaca gtaatcagtt
     1981 ccagaaatgc tcccagactg gtatacaacg ccggcacaaa accgtgcacc agaaactgaa
     2041 tgaccgttac gaacgaagca atgattacga tatagcaggg gagtttcacc tgctccggaa
     2101 tcactttccg cagcagagag atcacgatat tggaacagat cagcacgaac atggtagaca
     2161 agcccatgcc gatgccgtta aatgctgcgg tggtaactgc cagcgtcggg cacatgccca
     2221 gcagtcctac cagcgtcggg ttttctttca ggataccctt ggtaaattct tttccgtagt
     2281 tcatgcaccg gtcacctcct gattggcatg tacagttgca agggcacagt tcacagcggc
     2341 tttcatgccc ttggaagaat aggtcgctcc ggtgatattg tccacgccgt ctactgcgtc
     2401 actgtccgac agtcccacca gatgatctac cagcagttcc ggattggact gcacctttgt
     2461 gcctagtccg ggggtttccg aaacggtgac aaaggaaacg ccggtaacgg ctccgccctt
     2521 gtccacgccg atgacgacct gcaggccgcc tttgttatag ccgtctgcgg tcaccagcac
     2581 ggcatactgt ccgttttcgt ccagatacag accggtggca gatgcctttt cgttattctc
     2641 cggctggaag tctgtgactt cctcaaatgt gccggcatcg ggcagctgtt tcagactggt
     2701 ctgaatggca tctgcctctg cggcagcgat cttgtccttg gtcaccatat tggcaagtgc
     2761 cagaaatgca cagcagactg cacagattat tgccagcacc agcggcggca gcaccatgga
     2821 ttttacagaa gatgtctttg cctctgccat gttacttcgc ctcctttttc actgcgggct
     2881 ttgccccaaa ggggtgagtc atggtcagct tgtcgatgta cggagtcagc aggttcatca
     2941 gcaggatcga gaacgagcag ccctccggca tgttggcaaa ctcccgaatg acaaaggtca
     3001 gcaccgcaca gcccagaccg aaaatcagtt ttcccgcacc ggtcatgggg gtggtcacat
     3061 agtcggtcgc cataaagaat gctcccagaa ttacaccgcc gctgagcagg gcgtagagca
     3121 cgtcggtcat ttcgccgccg gtggcgaggg tatagaacag ttccagcact gccagaccgc
     3181 ccagatatgc cacaggggtg tgccacgaga tcagccccag caccagcagg aacacgccgc
     3241 cgatgagcag agccagtacc gaggtttcac ccagcgaacc gcccacgttg ccgaggaaca
     3301 agtccagcag accggcatca ccggaagcca gcggagtggc accggtcacg ccgtcgggga
     3361 ggtttccggt agcggcaaac atttcaaaga agcttctgcc gcgatagaaa aagggcttca
     3421 cccaagtggt catatcgccg ccgaaagaca gcagcaggaa cacacgtccg gtaatggcgg
     3481 gattggcgaa gttgcagccc agtccgccga acagctgctt gacgactgca atggcaaaga
     3541 cgcagccgat cgccgcttcc cacaggggca gcgttgccgg cagattcaga gccagcagca
     3601 cgccggtgag catggcactg ccgtcggtat aggtggtttc tttctgcatc agcttctgac
     3661 agaccatttc ggtgaacatg ctgatgacag cacagaacac aaccagaaac aatgctcgcg
     3721 gtccaaagac caccgtcgcc gccacgatcg ccggcaccag agccagcacc accagcagca
     3781 tgatcttttg cgtggtcatg ccgcttctca cgtgaggtga cggagaaaca gtcaataatg
     3841 atgtcatggt ttttcctcct attttcatgg attggtcgga gagtctgggg gattttaaat
     3901 atgtgcagtt gtaatatgag gtatcgttct tttcagaagg atctcacaaa gcaatacata
     3961 ttaaatataa cacatcgagt aaaagatttc agtaaaaccc ctccaccgcc taacggcggt
     4021 ccccctcgta ctgagcggtc ccctctgccc tgcgggcatc tccccacccc gtggggagtc
     4081 acccctttca ggggaggctt atttagttgt cactattata atgaatagcg gggcgatgtg
     4141 gcttgctatc cttgggcaag tctgccgtcg ccatttccgc tgggcgacgg gctgggcatc
     4201 gtcccctaca acaagggctg tcaactctcc ggatgccatg cttatttttt gatgctggac
     4261 atatagaatg cctttgccag ctggttggtt tctgccaccg gacgctttgc cggacaggcg
     4321 taagtacagc agccgcagtt catgcacagg gtgactttca gtttgcccag tgcttcgctg
     4381 tcctcctgcc ggaacgcctt ggcgatctcc gtgggcatca gccgcagggg acaggcgtga
     4441 acgcatctgc cgcagtgaat gcaggcggtg ggtttcgggg aaacgaattt ctccagagcc
     4501 agcacagcgt tattggcttt cagaatcggg tcggcagtgt ccggcacagc gatgcccatc
     4561 atggggccgc cgtaaagcac tttctttgcc cgttccatat cgcactgtgc aaaatccagc
     4621 acgtcctgca ccggtgtgcc cataggcacc atgacattgc agggcttgcc tacagcatcg
     4681 ccgtccaccg tcaccatacg ctgcaccatc ggcatgccgg ttttcaggta gcggctgaga
     4741 aatgccacgg aggacacgtt catcacgatc acgccctgat ctgccggcag ctgtccctcc
     4801 atgacggtgc gtcccagtgt gtgatagatc agcacctttt ctgcaccctg cgggtacgtg
     4861 gtggggagag agaagatctg aatctccggt tcggacttgg tcttttccgt caggacgcgg
     4921 atcgcctccg gcttattggt ttcaatgcca atgactgcct tggggatttg cagccagtgc
     4981 agcacctgcc gcacgccgtc cagcacttcg tccggtgctt cgatcatctg gcggtagtcc
     5041 gaggtgatat agggctcaca ctctgccgcg ttgatgacca gcgtgtccac ggtctgtttg
     5101 gggttcagct tcacgtgagt ggggaagctt gcaccgccca tgcccaccag tccgctttcc
     5161 cgcactgcct tgaggaaact ctctttatca gtgacagcgg gcggacggac atccggagaa
     5221 acagtctgtt cgccgtcggt ttcgatctcc accatgggac atgtcctgcc gttggacata
     5281 cggtaggtgg aaactgcctt gactttgccg gaaacaccgg agtggatcgg cactgccatg
     5341 aatgcgtcgg tatcgccgat cttctgcccc accagcacgg tatcccgcgg ctttacaaga
     5401 ggtgtacagg gtgcaccgat atgcatgctc atgggaatcc gcaccagttt cggcagcggc
     5461 agcggtacag aagcactgtt ttctgtgttt ttatggtgtg ccagatgcac gccatgcagt
     5521 ttcttcatag aatacagctc ctttcacttt ttcggattgc tttactttag gaaacactga
     5581 ataaagcacg cctgacggct ttgcacgcaa tctgcgtgca gtgtttcctt tatatatcta
     5641 cagaaacgga gtggtttctg cgataggcgt tgggaatgat ttatagggtg ggaatttttt
     5701 tcttcatgtg tgaaattcca cagaagaatc cctccaccag cttcgctgat ccccctcgta
     5761 ctgagcggtc ccctctgccc tgcgggcatc tccccacccc gtggggagtc acccttttca
     5821 agggaggctt atttctatag ttttagctgt tcatcgtatt acaatgtaca ggagttttac
     5881 gcattggggg tgaagtgcag cccgtcgctg acgtacagct tgccggactg gtcttgtgct
     5941 acgatctgat aatcctctgc gtgaaggtgt tcctccagat gctctgtgcc ctccagccag
     6001 atcagcgtgg agagaaagtc ggattgcagc ccgctgtcac agaacaccgt cactgtggtc
     6061 aggtcggatt ccgccggcat gcctgtctta gggtcgagaa tgtggcagta ttgctttccg
     6121 tcatcagcaa taaagtaccg ttcgtatccg ccggaggtgg acaggaaaca actgtcagtt
     6181 gacacggtgc ccagcgtacc gtcgccggac gggtcacgga tctcgatctg aaacggtttc
     6241 ccgtcaggct tctccccgta gcacaaaatg gaagatgtca tggaaactgt gccatagctg
     6301 gcggtgccgg actggtcgag agcctctttc acgcagtcca gactgtagcc ctttcccacg
     6361 gctccgcagt ccagctgtgc tccgtcggtg agggtcacgg tgccgtctgc cgccgtcacg
     6421 tggtcgggag aaatggtttt cagtaccggt tccagttccg ccgccgtggg cacgtggggc
     6481 gtatctgtgg taatgcccca cagtgccgtc agctgtccca cagtcaggtc tacactgtcc
     6541 ccgaatcgtt tttgcagggc agccgtttcg gacaccagct ggtatatggt atcgttccca
     6601 gaaattgtgc cgctctgatt cagccgggag atcgcactgt tttcctgata attgtccagc
     6661 gtcttgtcca gcgttttcag cagcgaagcc gtttcggaaa cgccactgcc gtggagagaa
     6721 atggtacagg cggtatccat tgcccagacg gtttcggtct gagccggctt tgcagaagtg
     6781 catgccgtca gcaccagcgg cagcagcacc gcaaaaagtt ttttcccgtg catcataaaa
     6841 acatctttct tttccgaggg aaatgtgata taataagggt aggcatttcc ggacggaact
     6901 tgtccgattt cttcaaaaat gcctaccctt tattataata caagccgttt attttgtaaa
     6961 cggtaaataa ttgtttattt tacagcgaaa ttattacctt tttcgcaaaa tttccgaaga
     7021 tgtatcgact atccaacgaa ttccacaatg gaggtttatt ttttatgaaa ggttaccgca
     7081 tcagcgtgat ccgccacggc agaactgccg caaatgacaa aggcatttac atcggcagaa
     7141 ccgattttcc gctttccgac aaaggggcgg cagagcttgc cggtaaaatg gacgagttcc
     7201 ggtatcccaa cgtggcgagg gtgtacagca gcccgctgcg tcgctgcacc gaaaccgcgg
     7261 aaattctctt tccggacgta cagatccaga cggtggacaa tctcattgaa atggatttcg
     7321 gcacatttga aggaaaatcc gctgacgaac tggtacacaa gccggagttc aaggagtggc
     7381 tgaaaggcgg tatggactgc cgtccgccgc agggcgagag cgtcaaggaa gtgcagctgc
     7441 gaatcttccg ttcgctgcgg gagatcattc gggacatgat gcaggaggat ctgctccact
     7501 gtgccgtcat tacccacggc ggcatgatcg ccaacatgct ggcagggttc gggctgccga
     7561 aaatttcccc gcaggagtta cagtgccagc cgggagaggg cttcgacatc tacgtcaccg
     7621 ccgacctgtg gcagcgttca caggcgtttg agattctggg catggtgccg tattcccgat
     7681 gaggatactt ttataatagt cggaaatata atagtagaaa accgcaccct ttgccggagt
     7741 tttccggaaa agagtgcggt ttttgatttt gatttgacaa actgcggcaa gtttggggtt
     7801 ttataggcaa taccgcatga attcttcgaa tgaaagaaag ttttcgtgtg catatgaaaa
     7861 cgatttcgca cggagcacat attatttcga aaaagcttgt tttttttcaa aaaggcttgt
     7921 attttcctgc atattatgat ataatgtgtt tattcctatc gaaacgggaa tttcggcggg
     7981 atttccgaga tttccgagat ttccgaagac aatacttccg gtacggcacc ggtttttcaa
     8041 gaaagggtta tacacatgac gtatgaagaa gcattgactt atctgactgc ccacggtcag
     8101 cagcacctgc tccggtatta tcccactctg acaccggcac agcaggaaca cctgctggaa
     8161 cagatctcca acctgcatct ggaacgcccc cagttccaga atcgcgaagc ggcggcacag
     8221 cagcggggac actttgcacc gctgggagct gtgacactgg aacagatcgc agaaaaggcg
     8281 gacgaatacc gcaggatcgg cttacaggca attcgtgatg ccaaagtggg agccgttctg
     8341 ctggctggtg gacagggctc ccgtctggga ttcgatcacc ccaagggcat gtacaacatg
     8401 ggcaagacac gggatctgta catctttgaa tgcctgatcc acaacctgaa aaaggtcact
     8461 gacgaggcgg aggcgtatgt gccgctgatg atcatgacca gcgaacagaa tgatgcagaa
     8521 acccgtgcat ttctgaaaga acacgactac ttcggctaca gtgcggacaa cgtacacttt
     8581 ttcaagcagg aaacggaacc ggcagtggac gaaaacggca aaattttcct gaacgctccc
     8641 gatcagatcg tactttcccc caacggaaac ggcggctggt tctcctcgat gcagagagcc
     8701 ggtctgctgc aaacgatcga ggagatcggg ctggaatggc tgaacgtctt tgctgtggac
     8761 aatgtgctcc agcagatcgc cgatccggta ttcatcggtg caaccatcgc ttccggctgt
     8821 gcctccggcg gaaaggtcgt cagcaaggca gatccggacg aacgggtggg tgtgctctgt
     8881 ctggaggaca acaagccttc gatcgtggaa tactacgaaa tgaccgaaga aatgcggaca
     8941 ctgcggaacg aggacggctc gctggcgtat cggttcggcg tgattttgaa ctacctgttc
     9001 cgcgtagatg ctctgatgca tacgctgggc tcggatttcc cgctgcacat cgcacacaaa
     9061 aagatctctc atctcaacga agcaggggag ctggtgaagc cggaggaacc caatggctac
     9121 aagttcgaaa cgctggtgct ggatatggtg cacatgcagg acagctgtct ggcgtttgag
     9181 gtagaccgca gcaaggaatt cgctccggtg aaaaatgctg tgggcgtgga ctctgtggaa
     9241 accgcccggg cactgctgga gcagaacggt gtcgtactgt aaccgatacc ggaaatgatc
     9301 cgattctgaa agagggcaag acggcttgtc ctcttttttg ttttccctag ggcaataccg
     9361 cacaaagatt gtgcggcaga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 naaagtcgct agacgctctt tgtgcggtat tgccctaggg caacattgcc caaaaaatga
     9541 ggaattacag aaaaataaat tgacaaacta cgaaaattgt ggtataatgt atagagtatg
     9601 ttctgcgttc tttcgcatgc atctgacagc atcaagcatg ctttgaaaat taaaaagcag
     9661 tactatgcac agcttataca gtgctgctaa aaaagaaagg atttttcttt tatgaaaaaa
     9721 gtagtgattt taggcggcgg tcctgccggt ctgacagcag cgtatgaact gctgaaagac
     9781 accaaggacg aatacgaagt gaccattctg gaagaaagcg gcgtattggg cggcatctcc
     9841 cagacggttc gctatcacgg caaccgcatg gacatcggcg gacaccgctt tttctccaag
     9901 gacgacaccg tcatgaaatg gtggtcagac ctgatgccca ttcagggaca gccgtccttt
     9961 gatgataaaa aactgggacg ggaaaagccg ctggaagccg gtggccctga tccggaaaag
    10021 gaagacgtag tcatgctggt gcgtgaccgc gtttcccgca tctatttcaa caagcatttc
    10081 ttcgattatc cggtgactat gaacaagaac accattcaga gcatgggctt tgccaccacc
    10141 atgaaggctg gcttcagcta cctcggttcc tgcatctcca agaagccgga aaccaatctg
    10201 gaaaacttct acatcaaccg gttcggcaag gtgctgtaca gcatgttctt cgagggctac
    10261 acggaaaagt tgtggggcag acaccccagc gagatctccg cagactgggg tgctcagcgt
    10321 gtcaaggggc tgtctatccg tgccgtgctg aaagacatga tctccaagcg ttccggcaag
    10381 aagaacaaca aaaatgcgga aacctctctg atcgaacagt tctggtatcc gaagtatggt
    10441 ccgggacagc tttgggaact ggtcggcaag aaagccgagg aaaagggctg caagatcctg
    10501 ttccatcaca gcgtcaagac cattcacacc gaaaacggca aggtgacttc tgtagtctgt
    10561 gaaacaccgg agggcagcaa gaccatttcc ggcgacatct tcatttcctc tatgccgatc
    10621 caggatctga tcgccggcat ggacggctgc cagaataccg aggtgcagcg gatcgctgcc
    10681 ggtctgccgt accgcgactt tgtcaccgtg ggactgctgg tcaacaagct gaatctggtc
    10741 aatcagactg ggactaagac actgggcaat attgtaccgg attgctggat ctatgtacag
    10801 gacaagggtg tcaagctagg tcgtatccag atcttcaata actggtcgcc ttatatggta
    10861 gaaaagccgg aagacaccgt ctggatcggt ctggagtatt tctgtgccga aggggacaag
    10921 ttctggaaca tgtccgacaa ggagtgcgtg gacttcgccg tcaaggagct gaccaaaatg
    10981 ggcgttatct ccgagggagc cgtactggac gcacaccgcg aaaaagtcaa gaaggcttat
    11041 ccggcatatt tcgacaccta tgcacagttc gatcaggtag tggattatct gaacaccttt
    11101 gagaatctgt tctgcgtggg cagaaacgga cagcaccgct acaataatat ggatcactcc
    11161 atgctgaccg ccattcacgc agcagaagcg atcaagcacg attccacgga caagggtccg
    11221 gtctggaacg tcaaccagga aaaggaatat catgagcaga agtaagctct tttccaaaaa
    11281 cagcaaacgc accggcaatc gttctcacgg ctgccggtgc agctgtatct gcctgccggc
    11341 ggcggagtcc aaccggtatt ttcacctgct ggaacggcac ccatatctgt gcagtctgtt
    11401 cgtgtgtctg ctgctgaatg cactgtactt cgggggagaa gcgtatctgg cacaaaatgc
    11461 cgtgcttctg gaaacgctgg ctctgctggc ggcgggagca gcggtcttgt ggcggctggt
    11521 gcggtatcag ccccagaaga aaccggcggc ggtctgcgtc agtgtggtgt atacgctgct
    11581 ggtgctggct ggggcgtggt gttatcacga agccagacac tccggactgt gggtgctggt
    11641 cggcggctgt gctgtgctaa gcggactgtt ctggctcacc cgaaagcagt accacaacca
    11701 gcggcggcgg gtttcactgc tcctgctggg gacaagcttt ctgctgaaat ttttctacgt
    11761 gtgctataca cccattaccc gacggcagca cgatgtggga aaattcgggg acgcggacaa
    11821 ccacgccggc tacatcacct atctgttgca gcatcaccag ctgccggatt ttgatccgcg
    11881 gggacactgg caattctacc acccgcccct gcatcacatg atctctgccc tgtggctgtg
    11941 gctgtcggaa cacgtgttcg gcatcagcaa tgccgcggct caggagtccc tgcaaacgct
    12001 gtccctgttc tacgccatgg cgatcgtcat cactgcctac cgcattctgc ggcacttccg
    12061 gctggaaggc atggcgttgt atgtaccgct ggcggtggta gcatttcacc ccggcttcat
    12121 tctcttttcc ggcagcatca acaacgatgc gttgtcggtg gtgttcattc tgggggcagt
    12181 gctgtggacg ctgaaatggt acgagaaaca gacgtgggag ggcatcgtga aaattgccct
    12241 ctgcatcggg ctgggcatga tgacaaagct ttcggcggca atggtggcaa taccggtggc
    12301 ggcggtcttt ctggtggtgc tggtacggca gctgcggcag agaaactggc ggatcctcgg
    12361 gcagttcggc ggatttgcag tgatctgttt cccgctggga ctgtggtatc cggtgcggaa
    12421 tctggtgcgg ttcggcgtac cgctgaccta tgtgcaggaa atgccggaaa actctgtgca
    12481 gtatctgggg gaacagtcgt ttttctcacg gatcctggac ttttcgccgc atcaggtggc
    12541 aagcgttttc gaacagtggg tacagcgaac cggcggcagc tacaacgagt ataacccgct
    12601 gatcgccctg ctgaaaaacg ccatgttcgg ggagtatatc aacgagtaca cgctggattg
    12661 cagcctgtgg cgaatcctca ccggtgtggt gctgttctgg ctcaatgtgg tgctggcggc
    12721 ggcagcgttt gcggcaatgc tctggctctg cggcaaacgg gaacagactg gcggacggct
    12781 gccgaagctg tttctggtgc tgttctacgc ggtgctgatg ggcggatttt atcagctttc
    12841 cgcggcggaa ccttttacct gttccatgaa ctaccgctac attacgccga cttgtgtcat
    12901 cggggcagtg tttctggggc tggcattcca gcggttccgg aacgggaaaa agcccgtgtg
    12961 ccgctggctc tgcggcattg gctggacgtt gtgcggctgc ttcgcggcat gtgcggcggc
    13021 gttttacatc gggctgggct tgattggcta gggaagtttt agaaatggga aaaccggcag
    13081 gggacaggct ctgccggttt tttgtttcac gtgaaacagt gggtggggtt gtgctttgcc
    13141 ttgtaaaagg ttctcccctg atatgtcaga cttcatggaa gaacctcttg gaatgtgtag
    13201 agggcgatgc ccacatcgcc ccgcaggatt gcccgaggac agtgattttc tagggcagcc
    13261 gtgtcgaaac cgtccggcgg aattcgctcg ttcctcgctg tccgccttgg gcttgccaac
    13321 gccggttgtt gccggtaatg ttggtattca agagttttct tgtgcaggtg gttaaccttt
    13381 atttgatgca gcgtttgcaa gttggtggag gggttctgtg aaatcttttg ttaatgtttc
    13441 agttacacag aagaatccct cagtcagcct gcggctgcca gctccctttt caagggagcc
    13501 taagtacaaa tgagcctccc ttgaaaaggg agggggaccg ccactaggcg gtggagggat
    13561 tctccgccac agcagccaac aggtttcata agaagcaaaa aaagaaaaga gttcgcattt
    13621 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaattggg gcgtaaggtc
    13741 agcggcgact cgccgcacgc cgcagaatag aaataatgca agttcctata gaatttgtac
    13801 ttgatttttt ctgcaaaatc cgctatgata aagttagcaa ctcaatacgc tctcacagaa
    13861 cggaggaatc tgatcatgac agaagaagaa aaaatgcttg ccggaaaact ctatgacccc
    13921 agcgacccca aactggatgc ccgccggctg ctggctcacc ggctttccat ggaatacaac
    13981 aacacactgg aaaccgatgc cgaaaaacgc aaagccattc tggcacagct gctgcccgat
    14041 tgcggagagg acacatttct gcaagggccg ctgcaatttg actacggctg ctatacccgc
    14101 ttcggcaaac gctgctacgc caacttcaac ctggtcgttc tggacgtgtg tccggtgact
    14161 ttcggcgatg acgtgttcat tggcccgaac tgctcgttcg tttctcctat gcacgccctg
    14221 ctaccggaag aacgcaacct gaaacagcgt ccggacggct cctactatga tctggaatac
    14281 ggcaagccca ttaccgtggg cagcaactgc tggttcggca gcaacgtcac cgtctgcggc
    14341 ggcgtgacca ttggtgaggg ctgcgtgatc ggtgcaggca gcgtggtgac caaagacatt
    14401 ccgccccatt cgctggcggt gggcaacccc tgccgtgtgc tgcgggaaat tacggaaaag
    14461 gacagcgtgc agtataaagg aatcactgca taaagcccgc ctgcatgcca atattataat
    14521 tggaaaatct cccgcacttc acacagaaag gacgattcat atggcagtga ttcgattgac
    14581 aaaggaaaat tttcaggcag aagtgatgca ggcggacaag ccggtgctga tcgacttcta
    14641 tgcagactgg tgcggtccgt gccagatggt ttcgccgatc gtggacgaga tcgccggcga
    14701 acgcagtgac atcaaggtct gcaaggtgaa tgtggacgaa caaatggaac tggcacagtc
    14761 tttcggtgtg tccagtattc ccacgctggt ggtgatctcc ggcggaaagc tggtaaagca
    14821 tgccgtgggt gcacagccga agtctgcgat tctggacatg ctggaagctg tagaatgatc
    14881 cacatattcc cgaggcaaca ccgcacaaaa cccgcctgac ggctttgcac gcaatctgcg
    14941 tgcagatttt tcgtcgtgtt gccccgaaaa aacaaagccg ctgtcctgtc agcttcgcgg
    15001 ggcagcggct tttttgcgga gatgcggcaa aagtccacgg gcaataccgc acaaagagcg
    15061 tctggcgact ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatctgtc
    15181 gcacaatctt tgtgcggtat tgcccacgaa atatacgatt ctgatatgat tttcatcaga
    15241 aaatgtacgt ttttg
//
