LOCUS       c00018_NZ_FNHQ..       15038 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_FNHQ01000048.1 Megasphaera paucivorans strain DSM 16981, whole
            genome shotgun sequence.
ACCESSION   NZ_FNHQ01000048
VERSION     NZ_FNHQ01000048
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:27:33
            Original ID  :: NZ_FNHQ01000048.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15038
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15038
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[6147:15038]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      6148..15038
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..15038
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /kind="single"
                     /product="OD_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15038
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="OD_fatty_acids"
                     /region_number="1"
                     /rules="(minimum(1, [PFOR_II, POR, POR_N]) and (minimum(2,
                     [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /tool="gutsmash"
     CDS             complement(3..356)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 98.1;
                     E-value: 1.9e-30)"
                     /gene_kind="electron_transport"
                     /locus_tag="ctg18_1"
                     /sec_met_domain="ETF (E-value: 4.2e-31, bitscore: 98.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLANYKGVYVIAEQFEGKLRNVSLELLGQGRILADKIGDEVGAV
                     LIGSGVKPLAQELIAHGAHKVYVYDDPKLEQYNTTAYTKVLQNFFEEVKPNVFLVGATN
                     IGRDLGPRVANVLK"
     CDS             complement(378..1181)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 179.4;
                     E-value: 2.1e-55)"
                     /gene_kind="electron_transport"
                     /locus_tag="ctg18_2"
                     /sec_met_domain="ETF (E-value: 4.8e-56, bitscore: 179.4,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEILVCVKQVPDTAEVKIDPVKHTVIRAGVPNIFNPFDQNALEAA
                     LQLKDEQGAKVTLLSMGPPQAVEVLREGLAMGADEAYLLTDRKVGGSDTLATGYCLAQA
                     VKKVAELQGLDHFDMILCGKQAIDGDTAQVGPQIAAELGIPQITYAAHIEVEGEKVTVK
                     QQNEEGYIVTEASFPVLLTAVKELNEPRFPTIRGTMKAKRREIPTLSADDIKVDESRIG
                     LSGSPTNVRKIFTPPQRTQGVILNNEDVAVSVATLMEKLTEQKII"
     CDS             complement(1367..1507)
                     /locus_tag="ctg18_3"
                     /transl_table=11
                     /translation="METVNTNTAAKIARRPLYIVYLKDFNVIGGKAEIMVAKEFIFCSN
                     I"
     CDS             complement(1550..1657)
                     /locus_tag="ctg18_4"
                     /transl_table=11
                     /translation="MNNAITNNAILHSSNTQYFLFYSIADVDILKKIKT"
     CDS             1877..3427
                     /locus_tag="ctg18_5"
                     /transl_table=11
                     /translation="MFFYIYIYTTNKRKDGIKLPTKNYALTLKNMVTLTQTKLHVLAEV
                     VGYDISYISKWSSGIKLPSSRCIEQVNEAMSLYFAKSIILHHKQENFCDTFNITAPIHD
                     GKELNIEIQRLLCAAYQASIKGNQLHKPKEKDSLFSKIVIGKLNATMFLSKLLQEELSQ
                     VNDTGDLLVFGEFCTLADMGLWNYFDSVQLNASRITIRVGLDVDKLDKKVKYMQHLYWT
                     LNAYLDYDFMFYDSQKLEHTNLIILKDRFVVQYSLNADDTITMCSCISDKDLVQTIYDE
                     FILSFVHQPCLITPMKSLGMNEMGFRTGFYATNHFSFFLTNGIEFLLPRKVFDSIINSI
                     DESTQSQDLVMQVERLRVTWEELLDRVDVDFIIPTTSIIRYIETGYIYLTDIEYNLTLD
                     ERKEHYTSILNAMKSNHNITMGIINSSVKATDYQGYNLSFYSNHSSCFFKKNKQYIHND
                     AYSFYVITNKRLLDIIQKGFDYLKKLPMYHTYSADEITKTYEMYQSLITRMLTLREKQ"
     CDS             complement(3479..4699)
                     /locus_tag="ctg18_6"
                     /transl_table=11
                     /translation="MGLLTKEQIRELIKERNMKTTSDISAMLKDLFSETMQEMLEAEMD
                     TTLGYGKNENAAKQTDNRRNGHSKKTVVSEYGDTELRIPRDREGEHEPLMVRKHQKNLT
                     GIEEQIIALYSKGMTVRDIQDHLNQLYGITVSPILISNVTNKLMPLIKEWQSRPLEKTY
                     AVVFLDAIHYKVRQEGAIVNKASYRVIGIDLDGHKDVLGIWIGEHETSKFWLVVLNELK
                     NRGVQDVLICCVDNLKGFNEAMGACFPEAEIQKCIVHQIRNSIRYVSYKDVKKVLADLK
                     PVYTAASEPLALEFLENFETIWGNKYPLIVSSWRANWSELSTFFKYPPEIRKIIYTTNM
                     MESYHRQLRKVTKGKSIFPNDESLQKMLYLATMDVLRKWTSRVQNWGQILLQLSVFFPD
                     KVKSALS"
     CDS             complement(4863..5327)
                     /locus_tag="ctg18_7"
                     /transl_table=11
                     /translation="LAAKKHILYRNDLQTEIAMQLAVKTEQLIKREADTMDFMTYKKYI
                     GSVQYNKKEKIFYGSVQGICEDIIFEGVSKKQLENNFHKVVDEYVNLCKKIGGNSRKKS
                     HRSVNIKISPEMYYKMYVTAVQKKISINQFIKKSIFDALGDSEIHNKSCI"
     CDS             complement(5612..5896)
                     /locus_tag="ctg18_8"
                     /transl_table=11
                     /translation="MITKRDLIYRQIGAKIRYYRILKGMTQAELAQQIHVNLHMLNEIE
                     CGKYDKNLLVTTLLDIADGLRVDVSAFIYFTEQEKWIACEILKNDNCEK"
     CDS             complement(6148..6807)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: CoA_trans"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg18_9"
                     /sec_met_domain="CoA_trans (E-value: 7.6e-29, bitscore:
                     90.5, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGLTGRALIASRCAKFFKNGDFVNLGIGVPLMCVNYLPEGVDIW
                     LEAEIGTVGSGAVPKWENVDVDVIDAGGQPASIIKGGSVYDHETSFAFIRGGHIDAAVL
                     GTLQVDQEGNIANWTIPGKFVPGMGGAMDLCAGVKKIIVATDHCEKSGKSKILKKCTLP
                     LTGANCVTDIVTEKCYFEVTPEGLLLKEIAQEYTVEDILACTEADVIVPDKIGVME"
     CDS             complement(6807..7583)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: CoA_trans"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg18_10"
                     /sec_met_domain="CoA_trans (E-value: 5.8e-55, bitscore:
                     175.9, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LNYYIRKVVIQLLSKVVSKEEALSKIHDGQTIMFGDWHGEFAPDE
                     IIDGMIEKGVKDIAAIAVSAGMPDQGVGKLIVEKRIKSLITTHIGLNPVAGEQMLAGEL
                     EIEFSPQGTWAERVRCGGAGLGGCLTPTGVGTEMEKGKQKLTINGKEYLLELPLHADIA
                     LVKATKADTAGNISFRMNSRATNSTIAYAADFVIVEVEDVVDVGELGPEEIDVPAPIVD
                     MIYVKTGEKKPLCPMWQRAKAKAEAKAKAKTEGGKK"
     CDS             complement(7607..8401)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg18_11"
                     /sec_met_domain="ECH_1 (E-value: 4.4e-62, bitscore: 199.5,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELQKLHYEVVNGIAVVTMDYSKNLNAIDEQMADELMYAVETAEN
                     DSNVKVVVIKGSTKAFSAGGDIGYFYQLIKAGGKVNMDGIIGKVGKITNGIKKMSKLVI
                     SSVSGSAAGAGVSLALTADFVICAENAKFIMAFVNLGLVPDTGGTYLLVKQLGEKRAME
                     LCATGRPLPAMEAKELGLVHKVVANEELDTATMKLAEKFANGPLISYKNIKRQIYAAGF
                     EDYKHYLDDCEIPTQRECAASADFCEGVKAFMEKRKAAFEGK"
     CDS             complement(8982..10718)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ILVD_EDD"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg18_12"
                     /sec_met_domain="ILVD_EDD (E-value: 9.1e-186, bitscore:
                     608.2, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKELYSQKMRILNAQGDALRLGSGWVPEDLGKPQVLIDTAFGD
                     SHPGSYHLNKLADFTKHALYASGCKPAVYTITDMCDGIGMATIGMSYSLASRELMTMMI
                     EVHAHGGPFDGIVLISSCDKSVPSHLMALARLNMPGIHIPGGSMLPGPEFQTSIAMYNM
                     GEMRNEGKVSDAELLRGQINSCPTCGACQFMGTASTMQCMSEALGMSLPGGALMPACTN
                     LIEQQAYRAGEQVKKLIEKNLTPRDIVTRESFENAIMVHGAISGSTNATIHIPAIAHEF
                     GIEITMDLFDEIHQKIPVLTSLQTSGKWPMQMLWYAGGVPAIMREISAYLHLDAMTVTG
                     KTVGENLETLEQSGYFKTAEGYLKNFGVGYRDIIHSVDKPYKSRGGTAVLYGNLAPKGC
                     IVKHAAIDPKMHVHTGPVKTFEDDNAASAAIYGGEIQPGDIVVIRYEGPRGNGMPEMLK
                     PTEAIYTCPELVSTVALITDGRFSGATRGPAIGHVSPEAAVGGPIALVEDGDIIHFDIP
                     NRKIDIVGIKGEPRTPEEVRDILIVRKAKWRPRPNTHKGAFRMFQELATDASEGAYLKF
                     DK"
     CDS             complement(10786..14325)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: POR"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: PFOR_II"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: POR_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_9"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) EKR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg18_13"
                     /sec_met_domain="PFOR_II (E-value: 3.4e-16, bitscore: 49.4,
                     seeds: 27, tool: rule-based-clusters)"
                     /sec_met_domain="POR_N (E-value: 4.1e-79, bitscore: 255.4,
                     seeds: 31, tool: rule-based-clusters)"
                     /sec_met_domain="POR (E-value: 9.1e-31, bitscore: 97.2,
                     seeds: 86, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.5e-11, bitscore:
                     34.3, seeds: 323, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_9 (E-value: 2.9e-11, bitscore: 33.4,
                     seeds: 61, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.4e-06, bitscore: 17.1,
                     seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="EKR (E-value: 3.3e-20, bitscore: 61.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGVTYMTKMKKIFKTMDGNTAAAHVAYAFTEVATIYPITPSSPM
                     AEHVDEWAAHGRKNLFGHTVKVSEMQSEGGAAGAMHGALQAGALTATFTASQGLMLMLP
                     NMFKIAGELLPGVFHVASRTVASNALSIMGENNDVMTVRTSGVAMLAESSVQEVMDLSA
                     VAHASAISSRIPFVNFFDGFRTSHEIQKIEILEDEDLRQFLDMDAVQAFRDRSLKPDKP
                     VLRGAVDAAEIYMQHRESINKFYMRVPEIVERYMRKVTELTGREYHLFNYTGAPDAEFV
                     VVALGSGAETTEEVVKYLAAQGQKVGCINVHLFRPWSEKHFLAALPKTVKRIAVVERTK
                     EPGSNGEPLYQSITATFATVKEAPEVYGGRFGLGSKDILPADIVACFDNLQSPIPKHNF
                     TLCITDDVCMLSLPRTSDIYVGGNGLKSCKFWGFGSDGTVGANKSAIKIIGDNTDMYAQ
                     AYFAYDSKKSGGVTVSHLRFGNSPIKMPYLIDKADFIACHRQSYVHQFELLQGIKRGGI
                     FLLNCTWQPEELDHKLPASLKQTIAKNDVQFYIINGMEIGRKLGLGGRINMIMQSAFFK
                     LANVIPLDLAIAKLKESVVTSYGKKGHKIVDMNNAAIDEGVKSIIKINVPVNWATAKDN
                     PSSKTSHSEYFKKFADPMNRMLGDTLPVSAFDGREDGTYPTGTTKEEKRGVAIFVPSWN
                     PDLCIQCNQCSFVCPHAAIRPFLTTPKEAEKAPAGFAAKDAVGITGMNYSIVTSVMDCL
                     GCGSCTYICPKHALTMKPFDDEEYKAEIWNYVIDLPIKPNPLKKTTVKGSQFEMPYVEF
                     TGACAGCAETPYAKILTQLYGNRMMIANSHGCSHVWAASAPTIGYTVNKKGQGPAWADS
                     LFEDAAEYGYGMFLGERSGRELLKGYVDKAIEAASPELKKALINWEDNMMVGEGTRERA
                     DKIIELLEKEKTGTPILEEVYKQRQYLVKRSQWLFGGDGWSYDIGYGGLDHVLAQGEDL
                     NVFVCDTEVYSNTGGQSSKATPTAAVAQFAAAGKKTKKKDLGMMAMSYGYVYVAQVAMG
                     ADKNQLLKAITEAEAYPGPSLIIGYSPCITHGLKVGQGQSQLEQKRAVEAGYWHLYRYN
                     PMLKEKGENPFILDSKKPSANFREFLMGEVRFSSLQKTFPETAEKLFEKTEKDAMERYE
                     SYVRLHDSLEIKK"
     CDS             complement(14337..15038)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg18_14"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.3e-52, bitscore:
                     167.1, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.5e-17, bitscore:
                     53.6, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HYILNGSKIFNTNGGEAEITVVFASTDKSKGAKGMSAFIVEKGMP
                     GFTYGKKESKMGIRASVQRELIFQNVKIPEENLLGNEGEGFKIAMMTLDGGRIGVGAQA
                     LGIAEGALEHAVKYAKERVQFGKPIAKFQSIGFMLADMKSKVEAARWLVYKAAFDMSRG
                     KNYSMSAAIAKKVASDAAMQVTTDAVQVFGGYGFTREYPVERFMRDAKITQIYEGTSQV
                     QQMIISGALLK"
ORIGIN
        1 gtcttcaata cattggctac gcgcggccct aaatcccggc cgatattcgt tgcccctacc
       61 agaaatacgt tcggttttac ttcttcaaag aagttctgca gtaccttcgt atacgctgtc
      121 gtattatatt gttccaattt cggatcgtca tacacatata ctttatgcgc tccatgtgca
      181 attaattcct gtgccagcgg ttttacaccg ctgccgatca agactgcacc tacttcatct
      241 ccgattttgt cggccaatat tctgccctga cctaacaatt ctaaagatac attgcgcagt
      301 ttgccttcaa attgttcagc gattacatac acgcctttgt aattagccaa atccatcttc
      361 acgtttcctc cttagaatta aattatcttc tgttctgtca atttttccat caacgtagca
      421 acagatacag caacatcttc attgttcaat ataacaccct gcgttctctg aggcggagtg
      481 aagattttgc ggacatttgt cggcgaaccg ctcaaaccga tacggctttc gtctactttg
      541 atatcatctg ccgacaaggt tggtatttcg cgacgtttgg ccttcatcgt tccgcgaata
      601 gtcgggaacc gcggttcatt taattctttt acagccgtaa gaagaaccgg gaacgaagct
      661 tccgtaacga tatagccttc ttcattctgc tgctttaccg ttactttttc tccttctacc
      721 tcgatatgtg ctgcgtaggt gatctgcggg ataccgagtt cagctgctat ctgcgggccg
      781 acctgtgctg tatctccatc aatggcctgc ttgccgcata agatcatatc gaaatggtcc
      841 agtccttgta attcagcaac tttcttgact gcctgtgcca ggcagtagcc tgttgccagg
      901 gtatccgaac cgccgacttt tctgtctgtc agcaaatatg cttcgtcggc tcccattgcc
      961 agcccttcac gaagtacttc tacagcctgc ggtggcccca tggagagcag cgttacctta
     1021 gctccctgtt catccttcag ctgcagtgca gcttccaagg cattctggtc aaacggatta
     1081 aagatgttag gcacacccgc acgaatgacg gtatgtttca ctggatcgat ttttacttct
     1141 gcagtatctg gtacttgttt tacacatacc aatatttcca tattacacta acctcgcatt
     1201 ctgccactta tggcataaat taattttaaa acacataaaa cctataaaat atatcttaca
     1261 attctatatt gatgctaata atacatttcc ccctttaaaa ttaagaataa tttgtataca
     1321 tactacatat actattaaat tatgatatta cataaaagtt tataagtcaa atattactgc
     1381 aaaaaatgaa ttccttggca accataattt cagctttgcc gcctatgaca ttgaaatctt
     1441 taagatatac aatgtataat ggtcgtcttg ctattttggc tgcagtgttt gtatttacag
     1501 tttccatatc aacactcttt tttttactta tatttctttc tacaaattat tatgttttta
     1561 tctttttaag tatatcaaca tctgcaattg aataaaatag aaaatattga gtattgcttg
     1621 aatgcaatat tgcattattt gtaattgcat tattcataaa tactgtttaa aatataatca
     1681 gttatgttat ataggtataa aaaaaatatt caaaaatcat gctactaata attaaaggtt
     1741 acatttaacc gaaaataaag tgattcaaat taatctcata tccaatcact ttattttcgg
     1801 tattttatgt gaaaatcatc acataaaata taatatattt atacgatccg cctaacttgt
     1861 taaaaacagc cctgtaatgt ttttttacat atacatatat actaccaata aaagaaagga
     1921 tggtataaaa ttgccaacaa aaaattatgc actaactctt aagaacatgg taaccctcac
     1981 acaaacaaaa ttgcacgtat tagctgaagt agtaggatat gatatttcct acatcagcaa
     2041 atggagcagc ggaataaaat taccatccag ccgctgtatt gaacaagtca acgaagcaat
     2101 gagtctttat tttgcaaaat cgataatcct gcatcataag caggaaaact tttgtgatac
     2161 ctttaacata actgcaccta tacacgacgg aaaagaatta aatattgaaa tacaacgtct
     2221 cctttgtgcc gcttatcaag cttcaatcaa aggaaaccaa ttacataagc caaaagaaaa
     2281 ggattctctt ttcagcaaaa ttgttattgg aaagttaaat gctaccatgt ttctttctaa
     2341 attgctgcag gaagaattaa gtcaagtgaa cgacactgga gatttacttg tatttggtga
     2401 attttgcaca ctagccgata tgggattatg gaactatttt gactctgttc agctgaacgc
     2461 ttctcgtatt acgatccgtg tgggcttaga tgttgataaa ctggataaaa aagtcaaata
     2521 tatgcagcat ctgtactgga cgttaaacgc atatcttgat tatgatttta tgttttatga
     2581 tagtcaaaaa ttagaacaca ccaatctaat aatactgaaa gacagattcg tcgtacaata
     2641 ttctctcaat gcagatgata ccatcacgat gtgcagttgt atttccgata aagatttagt
     2701 ccaaactatt tacgatgaat ttattttaag ttttgttcac caaccttgtc ttattacgcc
     2761 gatgaaatct ttaggcatga atgaaatggg atttcgtaca ggcttctatg ctactaatca
     2821 tttttctttt ttccttacaa atggtattga atttttactt cctcgcaaag ttttcgattc
     2881 tattattaac agcattgatg aatcaacaca gtcacaagat ctggtaatgc aggtagaacg
     2941 actgcgggtt acctgggaag aactactgga cagagttgat gtggatttta ttatccctac
     3001 cacctctatc atccgttaca tcgaaacagg atacatttat ttaacagaca ttgagtataa
     3061 tttgactttg gatgaacgga aagagcacta taccagcata cttaatgcaa tgaaaagtaa
     3121 tcataacatt accatgggaa ttattaattc ttctgttaaa gctactgatt atcaagggta
     3181 taatttatct ttttactcaa atcactcttc ctgctttttt aaaaaaaata agcaatacat
     3241 tcataatgac gcgtattctt tctatgttat cacgaataaa cgattactgg acatcattca
     3301 aaaaggattt gattatctga aaaaactgcc catgtatcat acatactctg cagatgaaat
     3361 aacaaagacc tatgagatgt accaatcttt aatcacgaga atgctaactc tgcgtgaaaa
     3421 acagtgatat ttttcaatag cagtagggtc tgtaaatagt tttgtgcttt tttcctaatt
     3481 aagataaagc agattttact ttatcgggga aaaagacgga taattgcagc aggatttgtc
     3541 cccaattttg aacccggctc gtccattttc tcagaacgtc catagtagct agatagagca
     3601 tcttctgcaa tgattcgtca ttagggaaaa tgctttttcc tttcgtcact ttccgaagct
     3661 gcctgtggta gctttccatc atattggttg tataaataat tttccgaatc tccggcgggt
     3721 acttgaaaaa ggtcgatagt tctgaccaat tagctcgcca agaactgacg atcaaggggt
     3781 atttattgcc ccatatcgtt tcaaagtttt ccaagaattc tagtgccagc ggctccgacg
     3841 ccgccgtata aaccggtttt aaatcggcca gtactttctt cacatctttg taggatacat
     3901 agcgaatgga attacgaatt tgatgaacaa tgcatttttg aatttcggcc tccgggaaac
     3961 aagctcccat cgcttcgtta aatcccttta gattgtcaac gcagcaaatg agaacatcct
     4021 gtacgccgcg gtttttcaac tcatttaata caactagcca aaactttgag gtttcatgtt
     4081 caccaatcca tatccccaaa acgtctttat gtccgtctaa atcaatgcca atgaccctgt
     4141 aggaggcttt gtttacaatg gcaccttctt gacgcacttt gtaatgaatc gcatccaaaa
     4201 agacaacggc ataggtcttt tctaacggcc ggctttgcca ttctttgatc aacggcatga
     4261 gcttattggt tacgttggag atcaaaatcg gcgatacggt aatgccatat agttgattta
     4321 ggtgatcttg aatatctctg actgtcatgc ccttgctgta caaggcaatg atctgttctt
     4381 caatgccggt caaatttttc tgatgttttc ttaccatgag aggctcatgt tcgccttcgc
     4441 gatcacgagg aatacgcaat tcggtgtcgc catattcgct gaccacagtc tttttgctat
     4501 ggccattgcg gcgattgtcg gtttgtttgg cagcgttttc atttttacca taccccaggg
     4561 ttgtatccat ttcagcttct agcatttcct gcatcgtttc actaaataaa tctttgagca
     4621 ttgccgaaat atcgctggtt gtcttcatgt tccgttcttt aatgagttcc cgaatttgtt
     4681 cttttgttaa gagtcccatg cgtttgacct ccctatcttc attctacaga tttttttcaa
     4741 aagcacaaac tattttacac tacctagcag tatcttaata tacaataaaa atcacgtcag
     4801 aatcataaat ttctgacgtg atttttattg aaaccatttt attacaaaca cttttatctt
     4861 ttttatatac aagatttgtt atgaatctca ctatcaccta acgcatcaaa aatacttttt
     4921 ttaataaatt gattgatact aatttttttt tgtactgctg ttacatacat tttataatac
     4981 atctctggcg aaattttaat attaacactg cggtgggatt ttttccttga atttccccct
     5041 atttttttac acaaattaac atactcatct actactttat gaaaattatt ttctaattgt
     5101 ttcttagata ccccttcaaa aataatatct tcacaaattc cttgaacaga accataaaaa
     5161 attttttctt ttttattata ttggacggat ccaatatatt ttttatatgt catgaaatcc
     5221 atcgtatctg cctctctttt tattaattgt tctgtcttta ctgccaattg cattgcaatt
     5281 tctgtttgca aatcatttct gtataatata tgcttcttcg ctgccaaact accgttttat
     5341 tcattgcgaa gtatcttcgc ttaactcata gcctttacta ttctccctca taaaatataa
     5401 aatctatttc ctctcttact tgcagattta acgtttaata taattataat cataccctat
     5461 ctcaaagcag ttatgatctt tttatatttt agttaactag ctaactaaca gtatagaaaa
     5521 taaccgtgaa aatgtcaatc aaattgcaac atagatacac tattctttac cacaaaacac
     5581 cttagcatac tgtatttcaa gatttatata tttatttttc acaattatca ttttttaata
     5641 tttcacaggc aatccatttt tcctgctccg taaaataaat aaaagccgat acgtcaacac
     5701 gcaaaccgtc agcaatatca agaagtgttg taactagtaa attcttatca tatttaccac
     5761 attcaatttc atttaacata tgcagattga catgtatctg ttgagccaat tcagcttgcg
     5821 tcattccttt aaggattcta taatacctaa ttttagctcc aatttgacga tatataagat
     5881 ctcgctttgt aatcattatg cgttatgcgc tccagcttat atgcccaata tattaaataa
     5941 aaatgttatt acctatgcta aatatatata ttattataac ataaaatata actctctata
     6001 taacattaat atgttttttt aaatgttctt attcaaatag attcatttaa caactttatt
     6061 ttgttatgta catcgcattt taataaaaat aagagataaa atcagtttta atgctgatct
     6121 tatcccttat ttttattaaa atttcgttta ttccattacg cctattttat ctggcacaat
     6181 gacatcggct tccgtgcaag caagaatatc ttcaaccgta tactcttgcg ctatttcttt
     6241 taaaagcaac ccttctggag taacttcaaa ataacatttt tctgttacga tatctgtcac
     6301 acaattcgca cccgtcaacg gaagtgtaca tttttttaaa attttcgact tgccgctttt
     6361 ttcgcaatga tccgttgcaa cgataatttt cttcacaccc gcacaaaggt ccatcgctcc
     6421 tcccattccg ggaacgaact tgccgggaat agtccagttc gcaatattgc cttcttggtc
     6481 aacctgaaga gtacccagaa cagctgcatc aatatgtcct ccccgaatga atgcaaaaga
     6541 tgtttcgtga tcatatacac tgccaccttt aataatagac gcaggctggc ctccggcatc
     6601 aataacatca acatcgacat tttcccactt cggaactgct ccgctgccaa cagtgcctat
     6661 ttcagcttca agccaaatat ctacgccttc aggtagataa tttacacaca tcagtggaac
     6721 ccctattcca aggtttacaa aatcgccgtt tttaaaaaac tttgcacaac gagatgcaat
     6781 aagtgcgcgt cctgttaatc ctgccattat tttttgcccc cttccgtctt ggcttttgcc
     6841 tttgcttcag ctttggcctt tgcacgttgc cacatcgggc acaacggttt cttttcaccg
     6901 gtttttacat agatcatatc aacaattggc gccggtacat caatttcttc cggaccgagt
     6961 tcacctacgt ctaccacatc ttctacttcg acaataacaa aatcagcggc ataagcaatt
     7021 gtactgttgg tagccctaga attcattcta aaagaaatat taccagccgt atctgctttc
     7081 gttgctttca caagtgcaat atcagcatgc agcggcaatt caagaagata ttctttgcca
     7141 ttgatcgtaa gtttttgttt gcctttttcc atttctgttc caacacctgt cggcgtaagg
     7201 cagccaccca gtcctgcacc accgcaccga actcgttcag cccaagttcc ctgcggactg
     7261 aactcaattt ccaattcgcc tgcaagcatt tgttctcccg ctacaggatt aagtcctata
     7321 tgtgtcgtaa tcagactttt aatacgtttt tcaacaataa gcttgccaac tccctgatcc
     7381 ggcatcccgg ctgatacagc aatcgcggct atatccttta cccctttttc aatcattcca
     7441 tcaataattt catctggtgc aaactcaccg tgccaatcgc caaacataat cgtttgtcca
     7501 tcatggattt tactaagcgc ctcttcttta cttactactt ttgataacaa ttggattacc
     7561 acctttctta tatagtaatt caaaatggac ttatttatct catatattat tttccttcaa
     7621 atgctgcttt ccgtttttcc ataaaagctt ttaccccttc acaaaaatct gcagatgctg
     7681 cgcattcacg ctgtgttgga atttcacaat catcaagata atgtttatag tcctcaaatc
     7741 cggctgcata aatttgtctt tttatgtttt tataggaaat aagaggacca ttagcaaact
     7801 tttctgctaa tttcatagta gccgtatcca attcttcatt ggcaactact ttgtgtacaa
     7861 gccccagctc tttagcttcc atagcaggta agggccgccc agttgcacaa agctccatcg
     7921 cacgtttttc gcctaattgt ttcacgagca gatatgttcc tcctgtatca ggaacaagtc
     7981 ctaaattaac aaaagccatg ataaattttg cattttcagc acaaattaca aaatctgcgg
     8041 taagggcaag gctgacacca gctccggcag cagatccgct gactgaagaa atgactagtt
     8101 tactcatttt ttttattccg ttcgttattt tgccaacctt accaataatt ccatccatat
     8161 taacttttcc gccagctttg atcagctgat aaaaataccc aatatcgcct cccgcagaaa
     8221 atgcttttgt tgatccttta attacaacta ccttaacatt ggaatcgttt tcagctgtct
     8281 ctacagcata cattaattca tctgccatct gttcatcaat tgcgtttaaa ttttttgaat
     8341 aatccattgt tacaacagca ataccattga ctacttcata gtgtaatttc tgcagttcca
     8401 taatgaacct ccttttcata atacaataac gtatgatttg tactttggaa tttaattttg
     8461 attatgaacg caagtaaagc ttattttaat aattaaaaaa tattttgaaa aaacattact
     8521 attccaaagc acctcgacaa ttgtcagatt cacattgcaa attttcgcca tatataatgc
     8581 aattaataca ttatacttat tcttttttca tatatgatat atcatctatg actatagtat
     8641 atattaacta gtgaaatatt gcaataacta aacgtttaac tagtttatat aaatattatt
     8701 tatataaact agttaaacgt tatttttaga atttaagttg atattttata ttttagttat
     8761 attttatgtt gttaaaacaa ctatattgtc accttatgtt tgtgattttt ttaacttttt
     8821 acttttatgt tataaattaa taatatttat taaatattat taattaacaa ggtaaggatc
     8881 aataccaact aacaaggtaa ctacataatg gaaacaagag ccatactaga taaatatcta
     8941 gtatggctct tgtttccatt aaaataatat tttttacact gtcacttatc aaatttcaga
     9001 tatgctcctt cagaagcatc tgtcgctaat tcttggaaca tgcgaaacgc tcctttatgt
     9061 gtattgggtc ttgggcgcca ctttgcttta cgaactatta atatatctct aacttcttcc
     9121 ggagtcctag gttctccctt tatccccaca atatcaattt tccggttagg aatatcaaaa
     9181 tgaataatat ctccatcttc tactaatgca attggtcctc ctactgccgc ttccggagac
     9241 acatgcccga ttgccgggcc ccttgttgct cctgaaaatc tgccatctgt tataagtgct
     9301 accgtagaaa ctaactctgg acaagtatag atagcttctg tcggtttcag catttccggc
     9361 atcccattgc cccgcggtcc ttcataccgt ataaccacaa tatctcccgg ctgtatctct
     9421 cctccatata ttgccgccga tgcagcatta tcatcttcaa aggtttttac cggtcctgta
     9481 tgtacatgca tctttggatc gatcgctgcg tgcttaacta tacatccttt gggtgctaaa
     9541 ttaccataca atacggctgt tccgccacgg cttttataag gtttatccac agaatgaatg
     9601 atatctcgat aacctactcc aaaatttttc aaatatcctt cagcggtttt aaaatatcca
     9661 ctttgttcta atgtttccag attttcccct actgtttttc ctgtaacagt catcgcatcc
     9721 aaatgcagat atgcactgat ttctctcata attgccggta cgccgcctgc ataccataac
     9781 atttgcatag gccatttacc gctagtctgc aaactcgtaa gaactggaat cttttgatgg
     9841 atttcatcaa acaaatccat tgtaatttca attccaaatt catgagcaat cgcaggtata
     9901 tgaatggttg cgttcgtcga tcctgaaatc gcaccatgta ccatgattgc gttttcaaat
     9961 gactctctgg tgacaatatc ccgaggcgtc aaattttttt ctattaattt tttcacctgt
    10021 tctcctgctc tatatgcctg ctgttctata agattagtgc aagctggcat aagggctcca
    10081 cctggcaaag acattcccaa tgcttccgac atgcattgca tcgtacttgc cgttcccata
    10141 aattgacaag ctccgcaggt aggacagcta ttaatttgtc ctcgcaataa ttctgcgtcg
    10201 cttacctttc cttcatttct catttccccc atattataca tcgctatgga tgtttgaaat
    10261 tcgggtcctg gcagcatgga acctcccgga atgtgaattc caggcatatt caatctcgct
    10321 aatgccatca aatgacttgg taccgattta tcacaactgg aaatcaatac aataccatca
    10381 aaaggtcccc cgtgagcatg aacttcaatc atcatcgtca ttaactctct ggaagccaaa
    10441 gaataactca tgccaatagt cgccatgcct ataccatcac acatgtcggt gatggtatat
    10501 actgccggtt tgcatcccga cgcatataac gcatgcttag taaagtctgc caatttattg
    10561 aggtggtaac ttccgggatg actgtcccca aacgctgtat caatcaatac ctgtggtttt
    10621 cctaaatctt ccggtaccca gccgcttccc agacgcagcg catcgccttg ggcattcaaa
    10681 atccgcattt tttgactata taattctttt gtgtccattt ttatcctcct tagtgggttc
    10741 aaaaatatgc aaatatctgt gttcagattt catatcatgc tgaatttatt tttttatttc
    10801 taatgaatca tgtaatcgta cataactttc atatcgttcc attgcatctt tttcagtttt
    10861 ttcaaaaagt ttttctgccg tttccgggaa tgttttttgc aaggaagaga accgcacttc
    10921 acccataagg aactcacgaa aattagcact aggcttttta gaatccagaa taaatggatt
    10981 ttctcctttt tccttaagca ttggattata tctatataaa tgccaatatc ctgcttcaac
    11041 tgcacgcttt tgttcaagtt ggctttgtcc ttgtcccacc tttaatccat gtgtaatgca
    11101 cggagaatac ccaataatca aagaggggcc tggatatgct tcggcttctg taattgcttt
    11161 taaaagttga tttttatcag ctcccatcgc tacttgagca acatacacgt aaccataact
    11221 cattgccatc atacccaaat ccttcttttt tgtttttttg ccggctgcag caaattgtgc
    11281 taccgctgct gttggtgtcg cttttgaaga ctgtcctcct gtattggaat atacttctgt
    11341 atcacataca aaaacattta aatcttctcc ctgtgccagt acatgatcca atcctccata
    11401 tccaatatca taagaccacc catccccgcc aaaaagccat tgtgatctct taacaagata
    11461 ttgtctttgc ttatatactt cttccaaaat gggtgtaccc gttttttctt tttcaagaag
    11521 ttctataatt ttatcagcac gttctcttgt cccttcgcca accatcatgt tatcttccca
    11581 attaatcaat gcttttttta gttcaggact agctgcttca atagctttat caacatatcc
    11641 tttcaaaagt tcccggccac ttcgctcacc caaaaacatt ccatatccat attctgctgc
    11701 atcctcaaat aacgaatctg cccaagccgg accttgtcct tttttattaa ctgtataccc
    11761 tatcgttggc gcacttgctg cccatacatg ggaacaacca tgtgaattgg caatcatcat
    11821 tcgatttcca tataattgag ttaatatctt cgcataggga gtttccgcgc accccgcaca
    11881 agccccagta aattcaacat acggcatttc aaattgactg cccttaacgg tagtcttttt
    11941 taatggattc ggttttatcg gtaaatcaat aacgtaattc caaatttccg ctttatattc
    12001 ttcatcatca aagggtttca tcgtaagtgc gtgtttagga caaatatacg tgcagctgcc
    12061 acatcccaaa cagtccatca ccgacgttac aatactatag ttcatgcccg taattcccac
    12121 tgcatctttg gctgcaaatc ctgcaggagc tttttctgct tctttcggtg tcgtcaaaaa
    12181 gggacggatg gcggcatgcg gacaaacaaa agagcattgg ttgcactgaa tgcataaatc
    12241 aggattccaa ctaggaacaa atattgccac cccacgtttt tcttctttgg tagttcctgt
    12301 cggataagta ccatcttctc gtccgtcaaa tgcacttacc ggcaaagtat ctcccagcat
    12361 tctattcatt ggatcggcaa attttttaaa atattcagaa tgagatgttt ttgaacttgg
    12421 attatctttt gctgtagccc aattaacagg tacattgatt tttatgatgg atttaacccc
    12481 ttcatcaata gccgcattat tcatatcaac aattttatgg cctttttttc catacgatgt
    12541 tactacagat tcttttaatt ttgcaatagc caaatccaac ggaattacat tggctaattt
    12601 aaagaaggcc gattgcataa tcatattaat acggccgcct aatcccaact ttctcccaat
    12661 ttccattcca ttaataatat agaattgtac atcatttttt gcaatagtct gtttcaaaga
    12721 agccggtagt ttatgatcta attcctctgg ctgccatgtg caattcaaga gaaaaatacc
    12781 tcctcttttt attccttgca gcaattcgaa ctgatgcaca taggactgtc gatgacaggc
    12841 aataaaatca gctttatcaa taagatacgg catttttata ggtgaattgc caaaacgcag
    12901 atgagatact gttactccgc ctgatttttt tgagtcataa gcaaagtaag cctgcgcata
    12961 catatctgta ttatcaccaa taatttttat tgcactctta ttggctccca ctgtaccatc
    13021 agatccaaat ccccagaatt tacaagattt caatccattt cctccaacgt aaatatcact
    13081 tgtacgcggc aaagataaca tacatacatc atcagtaata cataaggtaa agttgtgttt
    13141 aggtataggg gattgcagat tatcaaaaca ggcaacaata tctgccggca aaatatcttt
    13201 agaccctaat ccaaaacgac ctccatatac ctccggtgcc tctttcacag tagcaaaggt
    13261 tgctgtaatg ctttgataca aaggttctcc attagaacct ggttctttcg tacgttcaac
    13321 cactgcaata cgcttaactg tcttgggcag ggctgctaaa aaatgctttt ccgaccaagg
    13381 acgaaaaaga tgaacattta tgcagcccac tttttgtccc tgtgccgcta aatatttaac
    13441 aacttcttct gtagtttccg cccctgatcc caaggcgact actacaaatt ctgcatcggg
    13501 tgcccctgta taattaaata aatgatattc gcgtcccgta agttctgtaa ctttgcgcat
    13561 atatcgctct acaatttccg gtactctcat ataaaattta ttaatagatt cacgatgctg
    13621 catatatatc tccgcagcat cgactgcacc ccgcagaaca ggcttatccg gtttcaacga
    13681 acgatcacga aaagcttgca cggcatccat atcaagaaat tgtcttaaat cttcatcttc
    13741 taaaatttct attttttgta tttcatgtga agtacggaac ccatcaaaaa aattgacaaa
    13801 cggaatacgg ctggaaatag ctgaagcatg agctactgca gataaatcca tgacttcttg
    13861 cacactggat tcagctaaca tagctacgcc gcttgttcga accgtcatga catcattatt
    13921 ttctcccatt atgctcaatg cattagatgc taccgtacga gaagccacat gaaatacacc
    13981 tggtaataat tcccctgcaa ttttaaacat attgggaagc attaacatga gcccttgaga
    14041 tgctgtaaaa gtagctgtca atgcccccgc ttgcaaagct ccatgcattg ctcctgccgc
    14101 cccgccttct gattgcattt ccgatacttt taccgtatgt ccgaataaat tcttccgacc
    14161 gtgtgctgcc cattcatcaa catgttctgc catgggagat gaaggtgtaa tgggataaat
    14221 agttgctact tctgtaaatg catatgccac atgggccgct gccgtattcc catccattgt
    14281 tttaaaaatt tttttcattt ttgtcatgta cgtcacacct ttcatatata aataaattac
    14341 tttaataatg ctccggaaat aatcatttgc tgtacttgac ttgttccttc atatatctgt
    14401 gtgattttgg catcacgcat aaatcgttca accggatatt cccgcgtaaa tccataccct
    14461 ccaaaaacct gaacagcatc tgtcgttact tgcattgctg catcggatgc aacttttttt
    14521 gcaatggcag cgctcatact gtaatttttc cctcgagaca tatcaaatgc agctttatag
    14581 actagccagc gggcagcctc cactttagat ttcatatcag caagcataaa accaatagat
    14641 tggaacttag caattggttt tccaaactgc acacgttctt tagcatattt cactgcatgt
    14701 tccagtgctc cctctgctat gccaagtgcc tgagcaccta ctcctatacg gccgccatct
    14761 aatgtcatca ttgcaatctt gaagccttcc ccctcattgc caagaagatt ttcttccggt
    14821 attttcacat tttggaatat aagttcacgc tgtacggaag cacgaattcc cattttgctt
    14881 tccttcttac catacgtaaa cccaggcata cctttttcta caataaaagc actcatgcct
    14941 ttcgctcctt ttgatttatc cgtactagca aagactactg taatttcagc ttcgccacca
    15001 ttggtattga atatcttaga tccattcaat atataatg
//
