LOCUS       c00108_NZ_AFGF..       14620 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_AFGF01000116.1 Acetonema longum DSM 6540 Contig00121, whole
            genome shotgun sequence.
ACCESSION   NZ_AFGF01000116
VERSION     NZ_AFGF01000116
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:23:03
            Original ID  :: NZ_AFGF01000116.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14620
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14620
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2406:7030]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     minimum(2, [CoA_binding, CoA_binding_2, Ligase_CoA, PTAC,
                     Succ_CoA_lig]) and not (Gly_radical_scatole or Gly_radical
                     or Gly_430_pcre or Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_unknown"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2407..7030
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     minimum(2, [CoA_binding, CoA_binding_2, Ligase_CoA, PTAC,
                     Succ_CoA_lig]) and not (Gly_radical_scatole or Gly_radical
                     or Gly_430_pcre or Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_unknown"
                     /protocluster_number="1"
     protocluster    1..14620
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2185:4778]"
                     /cutoff="5000"
                     /detection_rule="(Fer4_7 and TPP_enzyme_C and POR and POR_N
                     and PFOR_II and not (EKR or Fer4_16))"
                     /neighbourhood="10000"
                     /product="porA"
                     /protocluster_number="2"
                     /tool="gutsmash"
     proto_core      2186..4778
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="5000"
                     /detection_rule="(Fer4_7 and TPP_enzyme_C and POR and POR_N
                     and PFOR_II and not (EKR or Fer4_16))"
                     /neighbourhood="10000"
                     /product="porA"
                     /protocluster_number="2"
     cand_cluster    1..14620
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Fer4_7 and TPP_enzyme_C and POR and
                     POR_N and PFOR_II and not (EKR or Fer4_16))"
                     /detection_rules="(minimum(1, [PFOR_II, POR, POR_N]) and
                     minimum(2, [CoA_binding, CoA_binding_2, Ligase_CoA, PTAC,
                     Succ_CoA_lig]) and not (Gly_radical_scatole or Gly_radical
                     or Gly_430_pcre or Gly_8-9_TMA))"
                     /kind="interleaved"
                     /product="porA"
                     /product="OD_unknown"
                     /protoclusters="2"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14620
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="porA"
                     /product="OD_unknown"
                     /region_number="1"
                     /rules="(Fer4_7 and TPP_enzyme_C and POR and POR_N and
                     PFOR_II and not (EKR or Fer4_16))"
                     /rules="(minimum(1, [PFOR_II, POR, POR_N]) and minimum(2,
                     [CoA_binding, CoA_binding_2, Ligase_CoA, PTAC,
                     Succ_CoA_lig]) and not (Gly_radical_scatole or Gly_radical
                     or Gly_430_pcre or Gly_8-9_TMA))"
                     /tool="gutsmash"
     CDS             3..1010
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pro_racemase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg108_1"
                     /sec_met_domain="Pro_racemase (E-value: 6e-128, bitscore:
                     416.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KLKNQRTLFAVDSHTMGEPLRLIVGGIPNIPGKSMPEKKEYFQKH
                     YDYLRKAVMLEPRGHNDMFGAVLTVPTIPEADFGVLFMHGGGFHNMCGHGTIATTTIAV
                     ETGMVAVKEPETIIRLEAPAGLVTAKVKVANGRAVQVSFENVPAFLYKKDVHVDIPGYG
                     KLTMDISFGGSFFAIINVKQIGIDICPENSSRLVETGMKIIEAVNQQVEIAHPELSHIK
                     TVDLCEIYGPAKSKDADMQNITVFDGQIDRSPCGTGTCAKVATLFAKGELALKQKFVYE
                     SVINTKFIGKALRETKVGDYPAIIPEITGSAYITGHSQFMIDEEDPVKYGFSLR"
     CDS             1141..2049
                     /locus_tag="ctg108_2"
                     /transl_table=11
                     /translation="MELDIVLILRIAITISALIFLVYFVKDCITHKDEFGKEHTFLYSV
                     IGFVTCVFDTLGIGNMATSMTAFRLTKSVKDEHLCGTGNVAFCITTIVEFILFVGLVKV
                     DTLTLVSMIVVAIIGSLVGVSIATRWSIVTVRKVLGVALTAVAIVMACRNLNVGPFGQM
                     GTATSLDGVSLALGLIGIFFLGALQTMGIGMFAPTMALVSMLGMSVTAAFPIMMGACSF
                     VMPWCSIKFVKEGKYNRKASVILTITGVFGVLLAYYVIKSLPLTTLIWVVVCVLLYTSF
                     TFFRQIAQKEDQQGESAQQQV"
     CDS             2186..2386
                     /gene_functions="biosynthetic (rule-based-clusters) porA:
                     Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1020:4Fe-4S binding domain (Score: 32.4; E-value:
                     1.8e-10)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_3"
                     /sec_met_domain="Fer4_7 (E-value: 1.1e-16, bitscore: 51.1,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 3.9e-11, bitscore: 32.4,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_2 (E-value: 2.7e-09, bitscore: 26.7,
                     seeds: 214, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_6 (E-value: 8e-11, bitscore: 31.6,
                     seeds: 134, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.3e-08, bitscore: 22.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKAIVRPERCQGCRYCIKACPKGAISIVSELNKKGLQPVVIDQE
                     KCIACGACYIVCPDYVFEIAE"
     CDS             2407..3501
                     /gene_functions="biosynthetic (rule-based-clusters) porA:
                     POR_N"
                     /gene_functions="biosynthetic (rule-based-clusters) porA:
                     PFOR_II"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_4"
                     /sec_met_domain="PFOR_II (E-value: 3.6e-22, bitscore: 68.5,
                     seeds: 27, tool: rule-based-clusters)"
                     /sec_met_domain="POR_N (E-value: 7.1e-54, bitscore: 172.7,
                     seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKELYKGNEALAEAAVRAGCNIYAGYPITPQSEILEYMSWRMPE
                     VGRVFVQSESEIAGISMVFGAAACGFRALTSTSGPGFSLKQEGISYIAACELPCVVVDV
                     ARYGAGLGDIWPAQGDYWQVTKGGGHGDYKVLAYAPATVQEMVDLTYTAFDKAEQYTNP
                     VIILSDGIIGQMMETVELPPMKTHDSKQFSWAMTGNKTGQHKIIMNPWMYSKEYSDKGV
                     PDGVPAYEKHLRDKYIAMFEQEQKWESIQAEDAEVVLVSYGISSRICKEAVSVARAQGI
                     KLGLIRPISISPFPKKAFERLANVKGFIAVEMSCLGQLEDDVKLASKCKIPVYSYLSGI
                     YVPETEQILSMVNDILSANGKEVG"
     CDS             3502..4239
                     /gene_functions="biosynthetic (rule-based-clusters) porA:
                     TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_5"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.3e-24, bitscore:
                     75.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LERRVPKTILQDSKYCPGCGHGVINRLVAEVLHEMNLEDKALCAT
                     AVGCACWVLEMIGVDQVQAQHGRAPAVATGMKRCCPDHAVFTYQGDGDASAIGLSETIW
                     AARRNEKIVQIYVNNGVFGMTGGQMSPTTLLGQKTSTCTTGRDESMHGEPLNVLNMMKT
                     LNIAYLARGATTNVPEIMKTKKYLQNAFEAQMKGEGFSFVEIVSPCPTNWNLSPLKSMD
                     RIADTVLKVYPLGEYIKRGEKLQ"
     CDS             4236..4778
                     /gene_functions="biosynthetic (rule-based-clusters) porA:
                     POR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_6"
                     /sec_met_domain="POR (E-value: 3.5e-39, bitscore: 124.5,
                     seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKTEIICAGFGGQGVLTTGLIIARNCAKSGKGVTWIPSYGPEMRG
                     GTANCHIKISNEEIFSPYVKEADVVIALNEASVNTFEKKLKSNGFLFVNSSIVPADRVY
                     RKDINVLLVPMTEMANEASNVKGSNLVMLGAFVKKTNIFDKEAFRNGIDDFFGSKGKIN
                     PANAVCFNLGWDYAQSC"
     CDS             4925..7030
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_unknown: CoA_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_unknown: Succ_CoA_lig"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_7"
                     /sec_met_domain="Succ_CoA_lig (E-value: 2.1e-47, bitscore:
                     150.2, seeds: 149, tool: rule-based-clusters)"
                     /sec_met_domain="CoA_binding_2 (E-value: 1.7e-21, bitscore:
                     66.7, seeds: 225, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VDLTKLLKPKSVAIVGASEKASTMGGDLLTNVKLWGKIENAYLVN
                     PNYDTIFEHKCYRSLENIPVNIDLVVLCTPKKTIPDLLRQAAKKGCGGAVVFASGYSET
                     RDAQGKQEEKELSALCKELGIALMGPNCGGFINYIDKVCGFAFIIEDRDRRGNVGFVSQ
                     SGQLCQSLMENTMKFSFMASVGNCCSVTVEDYMDFLVDDPDTKVVAAYIEGVKNPKRLA
                     EVLKKAAIKRKPIVVLKTGMSEKGSKLASSHTGSMSGADKVFDAVIKKFGVIRVKDLQE
                     LIAISTVLATLPELPKKPTFSSMAFSGGETVVCADVAYLNGVEFPDFNQSTTDAIQAML
                     PYYATANNPLDMTFTMSYDSDIFAKALRTIMDDPNIGMCLLCFTILQNNVTPDVHTMID
                     GIEKVVRAGNAKPMAFIPLLENTREPKIIERLGKLGVPVLPPTNYAFAVLKYLADYISY
                     SHFDKTLDLALPDIEEAAEAKGSKQTALSEFTSKELLKKYGLPVTGGAIAASEAEAVAI
                     AGRTGYPLVMKIESTDILHKSDVGGVVLNIDNEKAAVEAYRRILANAKKNAPNAKINGV
                     LIQEMAPKGLEMIVGVNNDPQFGPVILCGLGGIFVEIFKDVALYPAPLNKNEALKMLAS
                     LEAYPLLTGARGQKVLDVDALADAIVTISKYAADNKNTVSQVDINPLLVYPKGEGVRAV
                     DALVINK"
     CDS             7100..8089
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Shikimate_DH"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg108_8"
                     /sec_met_domain="Shikimate_DH (E-value: 9.1e-09, bitscore:
                     25.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLLLSKHDIQKVFSMKDAIEADKVAFRIFTEGKSVVPLRTNIPAP
                     KHEGTFLFMPAYAADLDCASVKIINIFPNNINKGIPSAPAQVLLIDGTNGFVIAVLDGT
                     YVTQLRTGAASGAAFDVLARKAAKKGALIGTGGQAAAQLEAMLCARNLQEVKVYDLNLE
                     RTKEFAAKMQAELKAYGTKIEAAASSDAAIDDADLIVTVTPSSKPVFDGTKVKKGTTVS
                     CVGSYQPHMQEMDPVILTRASKIYFDSQEAVLSEAGDILIPLANGTIAENDFTGELGNV
                     LLGNIPGRDHDDEIIVFKTVGIGTQDLVTAKCIYDKAVAMNIGAKWDA"
     CDS             8264..8476
                     /locus_tag="ctg108_9"
                     /transl_table=11
                     /translation="MPHISLMLYPGRTLATKEEFTKKLQALTVAELGCKMEQVSVSIEE
                     VARENWQKDVVEQVKPGSMIIQPNF"
     CDS             complement(8758..10254)
                     /locus_tag="ctg108_10"
                     /transl_table=11
                     /translation="MEHVKLIEKLEGYRRRNAVNHGYINCDLYRLFYDRDPELITAMRA
                     ESYLPQPNRTTMIPKKNGKMRKLSFPTGKDKLVQEAIRMILECIYEPTFSNLSHGFRPK
                     RSTQSAIAEIETWKGTIWFMEGDIAACFDEIDHRILEFILRERIHDERFIRLINKILKA
                     GYFDMQRNYHRTQTGNAQGSCCSPILCNIYLDKLDRFMEQIIERDTKGKYRRQNPEYTR
                     ARYQYHKALHNGSDPLLTESLLREWNKFSATDRFDPQFRRIRYVRYADDFLIGIIAPKS
                     YAVELKQQIKDFLQYKLALRLSDEKTVITHAAEHDVAFLGYILRKGYGKHNRFQNRPFD
                     PTLRILMNTEGILKRLREHGMCTASGYPIGITRLFRESPQDILQYANQVLRGLLHQQSG
                     CYNFSEGWRIQYIIQFSVAKTLARKFDISLKKVFARYGYSLRMHYKKRQGIHSLCRPCP
                     LPIICKAQELLLDRQIGSDIVPTAYLQPGQSAYRWLLHLR"
     CDS             10763..12433
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ILVD_EDD"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg108_11"
                     /sec_met_domain="ILVD_EDD (E-value: 2.4e-169, bitscore:
                     553.9, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDCTRSYWNGRKAVHRRTMMKGAGYCDSDIKTKPHIGVANTYMEG
                     SPGTAHLRTLVEKIKQGIWAAGGIPVEFGVPATCGNVANGAEELKYEQAARDIVAASIE
                     FVAKVHRFDGLVTVASCDNIIAGCYLAMARLNIPTLCVTGGSMTAGIYKGQKMVQAQLD
                     VAVLGGESEEVLLELEEHVCPSFGACPSMGTANTMQMTGEIINFVVPGTGTLPSTDNLK
                     LRRSKEAGEMIVEMTKADRKPSDLITRETLMNAIAFAMATAASTNIVLHLIAIANELGI
                     KITLDDFDHYASKLPCIVGVIPSGPYTVVDFHYAGGALTVMKMIESQIYTNVPTMDGRT
                     WKELLANVEAKSSDIIRTLADPLFKEPGLKILKGNLSPTGSIVRPTGVPAEMKQFSGKA
                     RVFSSDLDSFAAIERGEIVAGDVIVIRYEGCVGAPGMTELMVTTDALIAHGLHKSVGLI
                     SDARFSGFNYGAIVGHVTPEAAKGGLIALIEEGDIISVDTVQGTINLDVPENIIAERRK
                     KWVCPPPKVKTGLLALYAKNCRTADEGGAMQPWLDDETN"
     CDS             12888..14087
                     /locus_tag="ctg108_12"
                     /transl_table=11
                     /translation="MAGQVTQGMAATDNKGYGLETAIFVAIFSGILWYIQSIMGLSHML
                     STIMKTAYGLLQDTAFFVMAICVLAGASGGLLSEFGVVAVLQNMISPIMRPIFGLPGVA
                     SLGVITTFLSDNPAILALAQDKKIRKYFKKYEVPALTNIGTGFGMGLIVFTYMVGLGFA
                     KPAAIGVAGAAFGTLVSTRLMLYMTKRHYMKTGKFTAMDQLDDTLETVSEEESAVLHGM
                     KHKQSLFDRFMNAMLDGGKTGVVNGLNVIPGILTVCTAVMILTLGPATGADGNPTYIGG
                     AYEGIALLSKIGEYIYPVTNLLFGFQHGSNIAFPLTSLGAVGAALGTLPQMLTKGYVGP
                     NEIAVFTGIGMCYSGYLSTHVSMMDALGKRDLTGFAIISHTIGGICAGIAAHYMFLIIG
                     "
     CDS             14202..14585
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Ribonuc_L-PSP"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1038:Endoribonuclease L-PSP (Score: 82.8; E-value:
                     6.3e-26)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg108_13"
                     /sec_met_domain="Ribonuc_L-PSP (E-value: 1.3e-26, bitscore:
                     82.8, seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKVIKTKLAPEPTGPYAQGVLAGNFLFSTQMGICPATGELAGND
                     AESQTKQAMKNLKNILLEAEMNLADIGKMTIYIKDMNDHLPQVNQVCRSYFKEAPPVID
                     TIAATDLPIGGLVMIDIVAHKHQ"
ORIGIN
        1 aaaaattgaa aaaccagagg actttatttg ccgttgactc ccatacgatg ggcgagcctc
       61 ttcgcctaat cgttggggga attcctaata ttcccgggaa aagcatgccg gagaagaagg
      121 aatattttca gaaacactat gattacctgc gaaaggcggt catgcttgag cccagaggcc
      181 acaatgatat gttcggggcg gtcttgacgg tcccaacgat accggaggct gacttcggcg
      241 tcctgtttat gcatggcggc gggttccaca acatgtgcgg gcacggcacc atagccacca
      301 cgaccattgc cgttgaaaca ggaatggtcg cagttaagga accggaaacg atcatccgcc
      361 tggaagcacc ggctgggctt gtcacggcaa aagtcaaggt ggcaaacgga cgggcagtgc
      421 aggtttcctt cgaaaacgta ccggccttcc tgtacaagaa ggatgtgcat gtcgatattc
      481 cgggctacgg caagctgacc atggatatct ctttcggcgg cagcttcttt gccatcatta
      541 atgtcaagca gatcggcatt gatatctgtc cggaaaattc ctcccgcctg gtggagaccg
      601 gcatgaagat catcgaggct gtgaaccagc aggtggagat cgcgcatccg gaactttccc
      661 atatcaagac ggttgacctt tgcgagattt atggccctgc caagtcaaag gatgcggaca
      721 tgcagaacat taccgtgttc gacggccaga tcgaccgctc tccctgcgga accggcacct
      781 gcgccaaggt tgcgacgctt tttgccaaag gcgaactggc cctgaagcaa aagttcgtct
      841 atgagagcgt catcaacacg aaatttatcg gtaaggcact ccgcgagacc aaggtcggcg
      901 attatcccgc cattataccg gaaattaccg gcagcgcata catcactggt cacagccagt
      961 tcatgattga cgaggaggac ccggtcaagt acggattctc gctgcgataa tcccggagac
     1021 cgccacagtc aaagaaagac ataagcgggc ttcaaactgc atcggccgca agcctgcaat
     1081 atcgaacaac taaagaatat tacagggaaa acagaaaaac acgtaaggag ggcaacaatt
     1141 atggaattgg atatcgtgct tattttgagg atagcaatca ccatctcggc gcttattttt
     1201 ctggtgtact ttgttaaaga ctgtattact cataaggatg agtttggtaa ggagcatacc
     1261 tttctctatt ccgtcattgg cttcgttacc tgcgtatttg acacgcttgg catcggcaat
     1321 atggcaacaa gcatgaccgc gtttaggttg accaagagcg tcaaggatga gcacttatgc
     1381 ggtacaggaa atgtcgcgtt ttgtattact acaattgtcg agttcatctt gtttgtggga
     1441 ttagtgaaag tggatacgct tactttagtc agcatgattg tcgttgcaat cattggctct
     1501 ttggtcggtg tttcgattgc tacgagatgg tcgattgtga ctgtgagaaa ggtgctgggc
     1561 gtggcgctca ctgcagtggc aattgtcatg gcatgccgca atttgaacgt tggtcctttt
     1621 ggacaaatgg ggaccgctac atccctcgat ggagtcagtc ttgctctggg attaatcggt
     1681 attttttttc tgggggcgtt acaaacgatg ggaatcggca tgtttgctcc gacaatggcg
     1741 ctggtaagta tgcttggaat gagcgtgacc gctgcatttc cgattatgat gggagcttgt
     1801 tcttttgtga tgccgtggtg cagcattaaa tttgtaaaag aaggcaaata caaccggaaa
     1861 gcctccgtca ttcttactat tacaggtgtg ttcggcgttc tgctcgccta ttacgtgatt
     1921 aagtcattgc cgctgacaac attgatttgg gttgtagtct gcgtcctgct ctatacttcg
     1981 tttaccttct tccggcaaat tgctcaaaaa gaggaccagc aaggcgagag tgcgcagcag
     2041 caagtataat ggatggttta gtgaactcgt ttcagctagg ctgaaacatc ggtggttcgg
     2101 gtggagactg ctccacccga actggtgccg cctatagagg cgggagtctt gactcgctgc
     2161 agaagataag gaagggagat tcgtgatgtc taaagcaatt gtcagacccg aacggtgtca
     2221 aggatgcagg tattgcatta aagcatgtcc caagggcgcc atttcaatag taagtgaatt
     2281 gaataagaaa ggacttcagc ctgttgtaat cgatcaggag aaatgtattg catgcggagc
     2341 ctgctatatc gtttgcccgg actatgtttt tgaaattgct gaatagggag gataaggagg
     2401 agaaatatgg ctaaggagtt atataaggga aatgaagcgc tggcagaagc cgccgttaga
     2461 gcgggctgta acatatatgc cggatatccc atcacgccgc aaagtgaaat attagagtac
     2521 atgtcctgga gaatgcctga agtaggcaga gtttttgttc aatccgagtc ggaaattgcc
     2581 ggcatatcaa tggtttttgg cgcggcggca tgcggcttcc gggcgctgac aagcacatca
     2641 ggcccaggct ttagcttgaa acaggaaggt atttcctata ttgcggcctg cgaattgcct
     2701 tgtgtcgttg ttgacgtagc cagatacggc gccggattgg gcgatatatg gccggctcaa
     2761 ggcgattatt ggcaggttac caagggcggg ggacacggag attataaagt gttggcatat
     2821 gctccggcca cggtgcagga aatggttgac ttgacttata cggcttttga caaggcggaa
     2881 caatacacta acccggtaat tattttatcg gatgggatta tcggtcagat gatggaaact
     2941 gtggaattgc caccgatgaa aacgcatgat tccaagcagt ttagctgggc aatgacggga
     3001 aataaaaccg gccagcacaa gattatcatg aatccgtgga tgtattcaaa agagtatagt
     3061 gacaagggcg tgccggacgg ggtgccggct tatgagaaac atttaagaga taaatatatt
     3121 gccatgtttg agcaagagca aaaatgggag tcgatccagg ccgaggacgc cgaagttgta
     3181 ttagtaagct acggaatcag ttctagaata tgcaaagagg ctgtcagcgt cgccagagcg
     3241 cagggaatta aattaggctt aatacggcct ataagcatca gcccgtttcc taaaaaagca
     3301 tttgaacgcc tggcaaatgt aaaagggttc attgctgtgg agatgagctg tcttggccaa
     3361 ttagaagacg acgtcaaatt ggcaagtaaa tgtaaaatcc cggtatattc gtatttaagc
     3421 ggcatttatg ttcctgagac ggagcaaata ttgtccatgg tcaacgatat tttaagcgcc
     3481 aatggcaagg aggtgggcta gttggagagg agagtgccga aaacgattct tcaggacagt
     3541 aaatattgtc ctggctgcgg gcacggggtt ataaacagac tggtagcaga agtgctgcac
     3601 gaaatgaatt tggaagataa ggctttgtgc gctacggcag tgggatgcgc ctgctgggtg
     3661 ctggagatga tcggcgttga tcaagttcag gcgcagcacg gcagagctcc ggcggttgcc
     3721 accggaatga agcgatgctg ccccgaccat gcggtgttta cctatcaagg cgatggcgac
     3781 gcttccgcca tcggcttgtc ggaaacaata tgggcggcca ggcgcaatga aaaaatagtg
     3841 caaatttatg tgaataacgg cgtctttggc atgacaggcg gtcagatgtc gccgacaacg
     3901 ctgttaggcc aaaaaacttc tacctgcaca accggccggg atgaaagcat gcacggcgaa
     3961 cctttaaatg tcctgaacat gatgaagacg cttaatattg catatctggc gcgaggcgca
     4021 accactaatg tcccggagat catgaaaact aaaaaatatc tgcaaaatgc ttttgaagcg
     4081 cagatgaagg gggaaggctt ttcttttgtg gaaatcgtat ctccctgccc gactaactgg
     4141 aatttaagcc cgcttaaatc gatggataga attgcagata cggtcttgaa agtgtatcca
     4201 ttaggcgaat atattaagcg gggtgaaaaa ttacagtgaa aacagaaatc atttgcgccg
     4261 gatttggcgg acagggagtt ctgaccacag ggctgattat agcccgaaac tgcgctaagt
     4321 ccggaaaagg cgttacctgg ataccttcct atgggcctga aatgcgtggc ggcactgcga
     4381 attgccacat caagatcagc aatgaggaga tattcagccc atatgttaaa gaagctgatg
     4441 tagtgattgc gctgaatgag gcttcggtca acacatttga aaaaaagctt aaatcaaacg
     4501 gctttttatt cgttaacagt tccatcgttc ctgcagatcg ggtataccgc aaggacataa
     4561 atgttctact ggttcccatg actgaaatgg ccaatgaagc atcgaacgtc aaaggctcaa
     4621 accttgttat gctaggtgcg tttgtgaaaa aaacgaatat ttttgataaa gaagcatttc
     4681 gaaacggtat agatgatttt tttggcagca aaggaaaaat taatccggcc aatgccgtgt
     4741 gttttaactt aggctgggat tatgcgcaga gttgctaagc agtagctagc aggaaacggc
     4801 gttttgtaaa aggtgcattt attgagagat agtgcaacta tctctcaata aatgcacgga
     4861 tattcaagtt tggtgtgcca gtcaactttg ccggcaaaat gtaaaaacag gaaggacggt
     4921 aattgtggat ctaactaaac ttcttaaacc caaatccgtt gctatcgttg gcgcaagtga
     4981 aaaagccagc accatgggcg gcgacttgct gaccaatgta aagttatggg ggaaaataga
     5041 aaatgcctat ttggtaaacc ccaactatga tactattttt gaacataaat gttatcgctc
     5101 gctggaaaat attcctgtca atattgacct cgtagtgcta tgcacgccga aaaagactat
     5161 tcccgatctt ttgcgccagg cagccaaaaa aggatgcggc ggcgctgtag tgtttgccag
     5221 cggttacagt gaaaccagag atgcacaagg caaacaagaa gaaaaagaac tgagcgcgct
     5281 gtgcaaagag ttgggcatag ccttaatggg gcctaactgc gggggattca ttaactatat
     5341 tgacaaggtg tgcggttttg ccttcattat cgaagaccgt gaccgcaggg gaaacgttgg
     5401 ttttgtttcg caaagcggtc aattgtgtca atcgctaatg gaaaacacca tgaagttctc
     5461 ctttatggcg tcagtcggca attgttgttc ggtaacggtg gaagactata tggattttct
     5521 ggttgacgat ccggatacaa aagtggttgc tgcttatata gaaggggtga aaaacccgaa
     5581 gcggcttgcg gaagtactga aaaaggccgc aatcaagcgg aagccgattg tcgttttaaa
     5641 aaccggcatg tccgagaaag gcagcaagct ggcgtcatct cacaccggca gcatgtccgg
     5701 cgccgacaaa gtgtttgacg ccgtaattaa aaagtttggc gttattcgcg tgaaagattt
     5761 gcaagaactt atagctatct ccactgttct ggcaaccctc cctgaattgc cgaagaaacc
     5821 gacattttcc tctatggctt tctccggcgg cgaaacggtt gtttgcgcgg atgtagctta
     5881 tcttaacggc gtggaattcc cggatttcaa tcaaagcacc actgatgcga tacaggcgat
     5941 gctgccttac tacgctacgg ctaacaatcc tctggatatg acctttacaa tgtcctatga
     6001 cagcgatatt tttgctaaag cgctgagaac catcatggat gatcctaata tcggtatgtg
     6061 ccttttatgc ttcactattt tgcaaaataa tgttactcct gatgtgcata ccatgataga
     6121 cggcatcgag aaggtcgtcc gcgccggcaa tgctaagccg atggcgttta ttcctcttct
     6181 ggagaacacc agagagccaa aaattatcga aaggctggga aaactgggtg tgccggtctt
     6241 gcctccgact aattatgcct ttgcagtttt aaaatatttg gccgattata tcagctacag
     6301 ccactttgac aagactctgg accttgcgct gcccgacatt gaagaagctg cggaagccaa
     6361 ggggtccaag cagacggctc tgtccgagtt taccagcaag gaattattga agaagtacgg
     6421 cttgcctgtt accggaggcg ccatcgccgc aagcgaagcc gaagcggttg cgattgcagg
     6481 caggactggc tatcccctgg taatgaaaat tgagtcgacg gatatcctgc ataagtcgga
     6541 tgtcggcggc gtggtattaa atattgacaa tgaaaaagct gctgtcgaag cctaccgcag
     6601 aattttggct aatgcaaaga aaaatgcgcc aaacgccaaa atcaatggcg tattaatcca
     6661 agaaatggcg ccgaagggct tggagatgat cgtcggcgtt aacaacgacc cgcaattcgg
     6721 gccggtcatt ttgtgcggcc tgggcggcat atttgtcgag atctttaagg atgtagcctt
     6781 atatccggct cctttgaata aaaacgaagc cctcaagatg cttgcgtcgc tggaagctta
     6841 cccattgctc accggcgcca gggggcagaa agtattagat gtagatgctt tggcggacgc
     6901 catagtaacc atttcaaaat atgccgccga caataaaaac actgtcagcc aagtggatat
     6961 caacccgctg cttgtgtatc cgaaaggtga gggcgttcgt gcggtggatg cgctggtcat
     7021 taacaagtag cattatgagc gtgaacttaa aaactctgtt ttctagtggg cctgaattta
     7081 ctatataagg agaaaaaacg tgctgttact atcgaaacat gacattcaaa aagttttttc
     7141 catgaaagac gcgattgagg cagacaaagt ggcatttcgt atctttactg aaggcaaaag
     7201 cgtggtgccg ctccgtacca atattccggc gccaaagcat gaaggtacgt tcttatttat
     7261 gccggcctat gcggcggact tggattgcgc ctccgtcaaa attattaata tttttcccaa
     7321 caatattaat aaaggaattc catcagcgcc agcccaggtt ttgctgattg atggaaccaa
     7381 tgggtttgtc attgcggttt tagacgggac atatgtgacg caactccgga caggagctgc
     7441 gtcaggggcg gcatttgatg tactggcaag aaaagctgcg aaaaaaggcg ctttgatcgg
     7501 gaccggcggg caggcggccg cgcagttgga agcgatgctt tgcgcccgca atctgcaaga
     7561 agtaaaagta tatgatttga accttgaaag aaccaaggaa tttgcagcaa agatgcaggc
     7621 ggaattaaag gcatacggaa caaagattga agcggcggca agttccgatg cggcaattga
     7681 cgatgctgat ctgatcgtta cagttactcc ttcctcaaag ccggtttttg acggcacgaa
     7741 agtgaaaaaa ggcacaaccg tcagttgcgt tggctcttat cagcctcata tgcaggaaat
     7801 ggacccggtt atcttaacaa gagccagcaa gatttatttt gactctcaag aagccgtgtt
     7861 gtcggaagcc ggggatattt tgatccctct tgccaacgga acgattgcag aaaatgattt
     7921 taccggcgaa ctgggtaatg ttttattagg gaatatccct ggaagagatc atgatgatga
     7981 aatcatcgta tttaaaacag ttgggattgg cacacaagac ctggtcactg caaagtgcat
     8041 ttatgacaag gcggtggcaa tgaatattgg agctaagtgg gatgcttaaa ccaacttggt
     8101 gcgcttggtc aggtacggtt gctcttgtca tcagcttgat ttgatgggct atgttgccag
     8161 tcaaactcgc tatagaggcc ctgtattgtt tatcatgccg gcctttcccg caggtcgagc
     8221 gggaaatctt gcagcaaaaa tctatatagg ggaggggaag aatatgccgc acatttcatt
     8281 gatgttgtat ccagggcgaa ctttagccac aaaagaagaa tttacgaaaa aattgcaagc
     8341 tttgactgtg gcggaacttg gttgcaagat ggagcaggtg tcagtgtcca ttgaagaagt
     8401 tgcaagggaa aactggcaaa aagatgtggt ggaacaagta aaaccaggaa gcatgataat
     8461 tcaaccaaac ttttgaggga gtatgaaata ggcggattaa caaagtaacc cgccccttcc
     8521 cgaaccgtac aagcaacttt catcgcatac ggctctccat attccaatga atgatcaaat
     8581 tatcgtcgga taaattggtt gagctgcacc ctattcgcct cggctttggt aaagcaatca
     8641 tcacacaagg gaatctgccg ccgatggatt gccaacatga tggtgatcat gggtgagtaa
     8701 ggcatgtcga gcttcttctt ggtcttcctg tgatacatgc tgacatgacc ttgcggatta
     8761 ccgcagatgt agcaaccacc ggtaagcgga ttgaccgggt tgtaagtagg cagttggaac
     8821 gatgtcactg ccaatttggc ggtcaagaag aagttcttgt gccttgcaaa tgattggtaa
     8881 agggcaaggc cgacagagcg agtgaatccc ttggcgtttt ttgtagtgca tccgtagaga
     8941 gtagccgtaa cgggcaaata ccttcttgag gctaatgtca aattttcggg ccaaagtttt
     9001 agccacagaa aactgtatga tgtactggat acgccagcct tcagagaaat tgtagcagcc
     9061 agactgctga tggagcagtc cccgcaggac ctggttggca tactggagga tatcttgcgg
     9121 agattctcta aagagccggg tgatgccaat gggatagcca cttgccgtac acataccatg
     9181 ttcgcgtaac ctttttaaga tgccctccgt attcatcagg atacggaggg tagggtcaaa
     9241 agggcggttt tgaaacctgt tgtgtttgcc gtagcccttg cgaaggatgt agccgagaaa
     9301 ggccacgtca tgctccgcgg cgtgtgtaat aacagttttc tcgtcgctta agcgcaaagc
     9361 cagcttgtat tgaagaaaat ccttgatttg ctgtttgagt tctacagcat aggacttagg
     9421 ggcaatgata ccaatcaaaa agtcatcggc ataccggaca taccttatcc tgcggaattg
     9481 aggatcaaac cgatccgtag ccgagaattt attccattct cgtaacagag attccgtcag
     9541 caaggggtcg ctgccattat ggagagcctt gtggtattgg tatctagccc tggtgtattc
     9601 cggattctgt ctgcggtatt ttcccttggt atcgcgctcg ataatctgct ccatgaaccg
     9661 gtccagctta tcaaggtaga tattgcagag aatggggctg cagcaggagc cttgggcatt
     9721 tccagtctga gtcctatggt agttgcgctg catatcaaag tatccggcct tgaggatttt
     9781 attgatgagt ctgataaagc gctcatcgtg aattcgttcc ctaaggatga attctagtat
     9841 gcgatggtcg atttcatcaa agcaagcggc aatgtcaccc tccatgaacc aaatcgtccc
     9901 tttccaggtc tctatctcag caatggcgct ttgggtagaa cgtttgggtc taaacccatg
     9961 tgagaggttg gaaaaggtag gttcataaat acattccaag atcatgcgga tggcctcttg
    10021 aaccagcttg tcctttccgg ttggaaagct tagcttacgc attttaccat tcttcttggg
    10081 tatcatggtg gttctgtttg gctgaggcag gtaggattcg gcccgcatgg cagtgatcaa
    10141 ttccggatcc cgatcgtaaa acagccgata gaggtcacag ttgatatagc catggttgac
    10201 ggcattgcga cgacgataac cctccagttt ctctatgagt tttacatgct ccattcaaag
    10261 caactccttt caaaactagg aattggaata ctgcccctct tcaccatgtg ccatccattg
    10321 cagaaggccg ttgggctact atgagggctc tctgccatat accgaagcgg tacttaggca
    10381 agtcacgttt gacagcttca tatagtgcat ccatacgtag gtacccgttg gtcgttaccc
    10441 tgtttaccac aggtgtgtca atgagcctgc ttatccccaa tccttcctat atgggggata
    10501 cccattgata ccggtagttt actccggtag ctcacccagc cacagacccg gattcagccc
    10561 caaaggcttt accatatatg gcctgactcg ccaggttact cgacagcgga cgctgtctaa
    10621 tcctgacttt accgacatgc tatggtcccc cagaggcttt cgcgtggggt aagtcggttg
    10681 tcttacatcg catcgcagga gcgatgattt ctgtgaaata tatatgaagc gcacaacgtg
    10741 cgcacaaaag gaggagaaca aaatggattg taccaggtca tattggaatg gtcgaaaagc
    10801 ggttcatcgc cgcacaatga tgaaaggagc cggctattgc gactccgaca ttaagacgaa
    10861 gcctcatatc ggagtggcta atacgtatat ggaaggatct ccgggaacgg ctcatttgcg
    10921 cactcttgtt gaaaaaatta aacaaggcat ctgggccgca ggcggcattc ccgtagaatt
    10981 tggcgtgccc gctacctgcg gcaatgtggc gaacggcgcg gaagaattga aatatgaaca
    11041 ggccgcaaga gatatcgttg ccgcttccat tgagtttgta gcgaaggtcc atcgttttga
    11101 cggccttgta acagtggctt cctgcgataa cattattgcc ggctgttatc tggcaatggc
    11161 acggctgaac attccgaccc tttgcgttac cggtggttct atgacggcag gtatctacaa
    11221 aggtcaaaaa atggtgcagg ctcaattgga tgttgccgtt ctcggcggag aaagcgaaga
    11281 ggtgctgctt gaattagagg aacatgtctg tccttccttt ggcgcttgcc cttcgatggg
    11341 tacagccaat accatgcaga tgaccggtga gatcataaac tttgtggtgc cgggtacggg
    11401 tacgcttccc tcaacggata acttaaaatt aaggagatcc aaagaagccg gcgaaatgat
    11461 tgtggagatg acgaaggcgg accgcaagcc ttcagacctt attaccagag aaaccttaat
    11521 gaacgccatt gcctttgcta tggcaacggc tgcctcaacc aatatcgttt tgcatctgat
    11581 cgcgattgca aatgagttag gcattaagat taccttagac gattttgatc actacgccag
    11641 caagcttccc tgcatcgtag gcgtcattcc cagcggtcct tataccgttg tggatttcca
    11701 ttatgccggc ggcgctctga cggttatgaa aatgatcgaa tctcaaatct ataccaatgt
    11761 gcctactatg gatggccgta catggaaaga acttcttgca aatgtggagg ctaaaagttc
    11821 cgatattatc agaactcttg ctgatccctt atttaaggaa ccggggctta aaattctgaa
    11881 gggaaatctt tctcccaccg gctccatcgt tcgtcctacc ggagtcccgg cggaaatgaa
    11941 acaattctcc ggcaaggcga gagtgttcag cagcgattta gattcttttg ccgcgattga
    12001 acgtggcgag atcgtagcgg gagatgtcat tgttatccgc tacgaaggtt gtgtgggggc
    12061 acccggcatg actgagctga tggtaacaac ggatgcctta attgcccatg ggctgcataa
    12121 aagtgtcggt cttatttcag acgcgcgatt ctctggcttt aattatggtg ccattgttgg
    12181 tcatgttacg ccggaagcgg ccaaaggcgg tctgattgca ttgatagagg aaggcgatat
    12241 catttccgtt gacactgtgc aagggaccat aaatcttgat gtcccggaaa atattattgc
    12301 cgaacgcaga aaaaaatggg tttgccctcc gccaaaagtg aaaacaggcc tcctcgctct
    12361 ttatgccaag aactgcagaa cggcggacga aggcggcgct atgcagcctt ggttagatga
    12421 tgaaacaaat tgaaacaaat aagtaattta acagatatat gatagtatat tcccaaaaaa
    12481 ggggacaaag tttaatgaaa ctggctgctg ttaggcagaa attgtattaa aacggaaccg
    12541 tgtttttgta aacctccaga atgggtactc cagtttcatc agtctggagg tttacttata
    12601 tccacaagaa aaattgcccc agagcgccgg gccccaagaa aaaaaccgct gcattgcacg
    12661 gcagcggcgc ctgttgaaaa ggagacttac ttatgaatgg gaaaaagtgg tgtggacact
    12721 tggaaattca tcaaaactga cattgataaa gcacatcata aatgattgtg aagtatcaat
    12781 gagggtgcag acttggcttt gcccggaatg tggggtgtac ggatcggaca acgaaatggt
    12841 tcagccttat accaagttgg aagagaaatc tgagggggga gaaattcatg gcaggacagg
    12901 ttactcaagg gatggcagca acggataata aaggatatgg tttagaaaca gctatatttg
    12961 tcgcaatttt cagcggaatt ctttggtata tacagtccat tatgggtcta tctcatatgc
    13021 ttagtacaat catgaagact gcttatggct tgcttcagga tacagcattt tttgtcatgg
    13081 ctatctgtgt attggcgggg gcctctggcg gcttattatc tgaatttggc gtagttgccg
    13141 tattgcaaaa catgatatcg cctattatga ggcccatttt cggattgccc ggtgtagctt
    13201 cattgggcgt aataactact tttctgtctg ataatcccgc tattctggcg ttagcgcaag
    13261 acaaaaaaat ccgaaagtat ttcaaaaaat atgaagttcc tgcactaact aatattggaa
    13321 ccggctttgg gatggggctc atagtgttta cttatatggt gggccttggt tttgccaaac
    13381 cggcggctat tggggttgcc ggcgctgctt ttggcactct tgtcagcaca agattgatgt
    13441 tgtatatgac caaaaggcac tatatgaaaa ccgggaaatt tactgcgatg gatcaacttg
    13501 acgatacgct tgagactgtc agtgaagaag aatcggcggt tttgcatggg atgaaacaca
    13561 agcagtctct atttgaccgc tttatgaatg ctatgcttga tggcggcaaa accggcgttg
    13621 taaatggttt aaatgttatc cctggaattc tgactgtctg tactgccgtt atgattttaa
    13681 cgttaggtcc tgcaactggt gcggacggaa atccaaccta tattggcgga gcctatgaag
    13741 ggatcgctct tctatccaag atcggtgaat atatttatcc tgttaccaat ctgttatttg
    13801 gcttccaaca tggcagcaat atcgcgttcc cgctgacctc gctaggggcg gttggagccg
    13861 cgttggggac tttgccgcaa atgctgacga aaggctatgt cggtccgaac gaaatagcag
    13921 tatttactgg cattggaatg tgctatagcg gctatttaag tacccatgta tccatgatgg
    13981 atgctcttgg caaaagagat ctgacgggat ttgcgattat ttcacataca atcggtggaa
    14041 tttgcgcggg cattgcggct cactacatgt tcttaattat tggataaagc agtatagaca
    14101 tccaactgca gccagtactg gggaaattga taacggcgta ttgccgctat caatttcctg
    14161 gttttagtga attgaaaaaa gctaaacaag ggaggcaaaa catgagaaaa gtaataaaaa
    14221 ctaagttagc gcctgaacct acaggtcctt atgctcaggg tgtacttgcg ggaaactttt
    14281 tattttccac gcagatgggc atttgccccg ctactggtga attggctgga aatgatgctg
    14341 aaagccaaac caagcaggcg atgaaaaacc ttaaaaatat cctattagaa gctgaaatga
    14401 atcttgctga tattggaaaa atgaccatat acataaaaga tatgaatgat catcttcccc
    14461 aagtaaacca agtttgtaga agctatttta aagaagcgcc ccctgtgatt gataccatag
    14521 cagcaactga tttaccaata ggtggtttag tgatgataga cattgttgcc cataagcacc
    14581 aataaaatga taaagaaggg tgtattttaa aaaatatcgg
//
