LOCUS       c00019_NZ_JAHL..       17168 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JAHLNC010000019.1 Clostridium senegalense strain N2 O-25_ctg0019,
            whole genome shotgun sequence.
ACCESSION   c00019_NZ_JAHL..
VERSION     c00019_NZ_JAHL..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-13 23:41:57
            Original ID  :: NZ_JAHLNC010000019.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 4625
            Orig. end    :: 21793
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..17168
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[14625:19962]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..15337
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..17168
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..17168
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             1351..1875
                     /locus_tag="ctg19_3"
                     /transl_table=11
                     /translation="MKDKKSLIIILATLIVVIIGGIYYMKTNKSNKVSGFVQEEYSIKN
                     TTKKNFLIKIKEGYSEEIINMNLMDNFIKAVDSKKNDEIYIIEYERNENGEIVTSLKKL
                     NYDGSEIKVLEYDVSNQKEFKEGELIAYKKITKTGDNFGARIVGTTSEVQPIMEGDILF
                     EYVESNKVEYE"
     CDS             1977..5780
                     /locus_tag="ctg19_4"
                     /transl_table=11
                     /translation="MNNKLKKNLSKVMTVALLASVTVGSIPSMAKAEEVEKLLNMPLTV
                     ESKEQIINNFKNDLIKSDTIIKDNQNQEEVVRVIVEVNGKSAQEMIPRGLRPTEKESSQ
                     VEAAQKPVRDEVATIEGVEVRHSYTNVFNGFSAEVKRKDIKQIEEVNGVRKVTEAQKYT
                     EDMNSAKKLTQIEDVWKKYSLKGEGMVVAILDTGIDYKHKDFKNPEDTGKLKLKKDEVE
                     KVKASGVLKADKNANTYFTEKIPFGYNYADRNEQIVDLRDSKGPHGAHVAGIVGADGDD
                     KLIDTNESVKGVAPEVQLLAMKIFSNGPLGKYTYSDDQLAAIDDSVTLGADVINMSLGS
                     PAGYREDSDPVQDAITRATNSGVMVVVSAGNSSASTDPYGLGMLNDQITVGAPALAKDA
                     LMVASYENTTVAEKVIVFKDGKGKVVTEGSFKDHQVDYKDMLNKNYKIVDCGLGKAEEC
                     KDVKGNIALIKRGGLTFIEKIINAQNNGAVGVVIYNKDGDESLISMATDPNIKIPAICV
                     GNTTGKNLLNAMKKDTVLFNGDLKNATSENSNSSDFSDFTSWGPSPSLEFKPQVAAPGG
                     QILSTLNEGEHGVMSGTSMAAPHAAGSMALLMEAIKEYAPELKGRELIDYAKNIMMNTS
                     SVKIDKFAKNIPYSPRRQGAGLVQVEDAIRNRVTVTNNGEASVALKEIRGDKATFTLDL
                     KNYGSEDIEYKVEELGGVLTQDENTEYGEMVRDKALNKKDASLEFSSDKIKVPAKGNAK
                     VDVTLKIGSGLSEDRYLEGFIKFTPKNEKVPTLAIPYLGYYGDWGKESITTNNAWDEDK
                     HILVETLKSTGEYPGVLVENLAVTKVNAEDSIMGVVGKNEDKSNKYDSSKIAISPNGDS
                     MNDTIYPGLYLMRNAKTASVEIVDENGKLVRNLGTTENYRKKIISSESGTIPMIADDLA
                     WDGKVFDKSKGSYVNVSDGNYTYKVKLKVDYESAQEQVIEMPVKVDTVGPNVEVVKYEN
                     LEDGAIKLYFKANDELSGVNEKGEFPVVINGALNVEATKAAVTYDSKTGLYSKTITGLQ
                     ANTLNQIEIGAFDYANNLGGVSTVIPVGKIPPASITFDDKVFSQGQITVNQNEYTISGK
                     INRPIKDLIINGEKINLAINNDGTVNFSKSLQLSEGINVVNIKAVDYNGKVLQDHAYKI
                     SCDTIKPVIEIESPEVVDNKITATSDVVTLKGRVYDNLWGYKFYVNGELIKVSDYPTPV
                     GPEVNGYDFSVDVKDVKNGDFILLKAVDSNGNETELKLQVVR"
     CDS             6006..7919
                     /locus_tag="ctg19_5"
                     /transl_table=11
                     /translation="MIVLSCNKIHKSFGIDVILKDITFSINEGEKIGLIGANGAGKSTL
                     FKILTSQMEYDSGELFIDKSKKVGYLSQNLSLESDNTIYGELLLVFEELLNMERKMKTL
                     EEKMNEPYDEAKADYHNKLIKDYTTLTELYQHRGGYTYKGEISRVLKGLGFFEEDFEKP
                     ISILSGGQKTRVALCKLLLRKPEILLLDEPTNHLDLDAIEWLEEYLKSYKGSIIIISHD
                     RFFLDAVTNKTIEISYGMAEIYNGNYTAFIDLKKKNYEVALKAFSLQQAEIKRQEEIIQ
                     KYKSFNREKSIKAAESRQKALDKIERIEAPHKDQKASRINFETRIKSGNDVLYVEELSK
                     SYGEKVLFTGVNMDIKRENKIALIGENGRGKTTLFKIIMDEIQPDSGIKVLGKNVNIGY
                     YDQEQSNLDLNKTIIDEVWDDFPELTTTEIRTYLAAFLFRGDDVFKKIEKLSGGEKCRI
                     NLLKLMLSQNNFLLLDEPTNHLDIVSREALEDAILSYDGTVLVISHDRYFLNKVVGKIF
                     ELNEDGIKEYLGNYSYYIEKKNNPLRFEQEEQSSGITKTQIKLDKKKKREEEKKQREIQ
                     KYLKNLEENIATTESEIEKLQELLCKEEIYSNPEKSVEVNELLSEKEELLSNLYEEWEE
                     NL"
     CDS             8988..9629
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CoA_binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg19_6"
                     /sec_met_domain="CoA_binding (E-value: 1.1e-26, bitscore:
                     83.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LDRKKNISMAVIKRIPKYYRYLKELAENDVDRISSNELSEKIGFT
                     ASQIRQDLNCFGDFGQQGYGYNVKELLAQISSILGLDKEYNTIIIGAGNIGQAIANYTY
                     FQRMSFDLKAIFDINPKLIGLRIRDVSIEDIDNLESFIKNNEVDIAIICVPKERAQGVC
                     DIITSSGIKAIWNFAPVDLKVPSDVIVENVHLSESLMTLSYLLNESKLTE"
     CDS             10001..10906
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: EC1_2-faecali"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg19_7"
                     /sec_met_domain="EC1_2-faecali (E-value: 3.5e-90, bitscore:
                     291.2, seeds: 67, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQIYTSEDIIVIEVDGLQLLFLVKIVNYITICGKIFMGDSIMEF
                     KNIILEKDGNIGIVKFNRPKALNALNSETLKELDNVIDLIEKDNEIYAVILTGEGKAFI
                     AGADISEMEGLNALEGRAFGILGNKVFRRLETLDKPVIAAINGFALGGGCELSMACDIR
                     IASAKAKFGQPEVGLGITPGFGGTQRLARLVGECKAKELIFTANIIKAEEAFNIGLINK
                     VLEPEVLMEEAMNMAKQICNNAPVAVKLCKEAINKGMQMDIDSALNYESEAFGMCFSTY
                     DQKEGMKAFLEKRDKNFKNM"
     CDS             11012..12151
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg19_8"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.5e-56, bitscore:
                     179.7, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 5.7e-31, bitscore:
                     96.3, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.3e-36, bitscore:
                     115.3, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFSLTKEQEFVRQMVREFAETDVKPIAAEIDITEEFPMENVKKM
                     ARYNMMGIPFSTEFGGAGGNNVSYAMAVEELSRVCGTTGVILSAHTSLCASPIDMFGNQ
                     EQKEKYLTPLAKGEKIGAFGLTEPNAGTDASGQQTTAVLDGDNYILNGSKIFITNGGVA
                     DVFIVFAMTDKSKGTRGISAFIVEKGFKGFSIGKVEEKLGIRASSTTELVFEDCIVPKE
                     NLLGKEGQGFKIAMKTLDGGRIGIAAQALGIAQGALDEAVAYMKERKQFGRSLSKFQGL
                     QWMIADMDVQVEAARQLVYKAAYNKDNKLPYTVEAARAKLFAANTAMDVTTKAVQIFGG
                     YGYTKDYPVERMMRDAKITEIYEGTSQVQKMVIAGSLLK"
     CDS             12165..12944
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 179.9;
                     E-value: 1.5e-55)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg19_9"
                     /sec_met_domain="ETF (E-value: 2.6e-56, bitscore: 179.9,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIVVCIKQVPDTTEVRIDPKTGTLIRDGVPSIINPDDKSALEEA
                     LRLKDSHGAHVTVVTMGPPQAQKALEEALAMGADEAILVTDRAFAGADTLATSHALAGV
                     IKKLEYDVIFAGRQAIDGDTAQVGPEIAEHLNLPQITYVEEVDVLEDGLKVRRAWEDGY
                     EYIKVKTPVLLTAIETLNEPRYMNIRNIFELGNKEIKVWTAKDIDVDVALLGLKGSPTK
                     VKKSWAKEAKGQGEIFTVSPKEAASIAIGKLKEKHLI"
     CDS             12970..13971
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 177;
                     E-value: 1.1e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg19_10"
                     /sec_met_domain="ETF (E-value: 2e-55, bitscore: 177.0,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 3.4e-38, bitscore:
                     119.3, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIADYKGVWVFAEQRDGELQKVSLELLGKGREIADKLGVELTAL
                     LIGHNVEDMAKELTAYGADKVLLADKEELAHYTTDGYAKVITDLANSHKPEVIFIGATF
                     IGRDLGPRVAARLSTGLTADCTALDIDPENKNLLMTRPAFGGNLMATIMCGDNRPQMST
                     VRPGVFEKLEKDTNKEVKIEKAVFELEKSDIRTQVLEVKKILKEVADIIEADILVSGGR
                     GIGCKENFDILKELADEIGGTLSGSRAAVDNGWIDKALQVGQTGKTVRPQIYIACGISG
                     AIQHLAGMQDSNYIIAINKDADAPIMQVADVGLVGDYQKIVPELVQQLKAMK"
     CDS             14519..15337
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg19_11"
                     /sec_met_domain="HCDH (E-value: 1.1e-39, bitscore: 124.6,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 1.7e-62, bitscore: 200.1,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAGIAQVLATKGYEVTMLVRNMDKFQNVNLAGINKTLTKLVTKG
                     KITEDVKGEILGRINGTLDMNDVKDVDLIIEAIAENMETKRHYFSKLDEICKPETIFAT
                     NTSSLSITEVASCTNRPDRVIGMHFFNPAAIMKLVEIIRGMATSDETFSAVKELTQSIG
                     KDPVEVKEGPGFVVNRILIPMINEAVGILDQGIATVEDIDKAMMLGANHPMGPLALGDL
                     IGLDICLAIMEVMYKETGDSKYRANSLLRKYVRAGYLGRKTKKGFYDYNK"
ORIGIN
        1 taagtccaaa tggtgacagg gttaatgatg ttatttatcc agcattatat ttaatgagaa
       61 atgctaaatc tgttaacgta gatattttag attcaaatgg aaaagttgtg agaactttag
      121 gagaagttaa taatttaaga aagaatttaa tagctacaga acaaggtcaa gttccaacag
      181 ttattcctaa tttagtttgg gacggaaaag tatttaatag ctctactggt agatttgtaa
      241 ctgcagcaaa tggacaatac acttatagag taaaaatgaa gatagattat attggagcaa
      301 aagatcaaat agttgatatt ccagtaaaag tagatactaa atctccttgg tttagtgctg
      361 aaaaatatga agatcttggg gatggaaagg taaaagttta ctttaaggca gtggatgaag
      421 agtcaggaat cttagataga gctaaattcc cagttttaat aaatggagaa ttaaataaag
      481 aggcaacaga tgctaaagta gaatatgata gtaacactgg attgtatagc aaagttataa
      541 atggattaga tccaaatggt aaaaatgaaa taacaatagg tattttagac tatgctaata
      601 atttaggtgg tggaacagtt tctatagatt tatcaaaaga agaacctaaa caagaactta
      661 aagaagaagg tagagacaat ctaattactt ttgatgataa gaattttgca gaaaataaag
      721 ttttatcaga caatgaaact tacaaaatag ctggtaaagt aaatgaaagc ataaatgaaa
      781 taataataaa tgatgctaag gtagaattag taaaagacaa ttctcaaaga ataatattta
      841 agtatgatgt taaattaaat gagggagaaa atctaattaa aattgtatgt aaagactcaa
      901 atggtaaggt agttatagaa aatcaataca aggttaatta cgaagtaaag aagtagaaga
      961 aaaagttaac taataattat atttttgtta acaaaaaaga ttttagaaga aattgctttt
     1021 gcaatttctt ctttaattta aaataaaatt aaagaagaaa caaaaatatg aagtgatagt
     1081 atcatttaaa aatataaaat gtataatttc attcaattat tattaatata aaaaataatt
     1141 tctaataatt tacatcatta atgaacgaaa gataaaattt tctttaactg tattgtaaaa
     1201 aaaatacttt aaagtataat gtttatataa tttactgtga aagtaaaagg tataaagaga
     1261 ggtaaaaata aatataattt attatgtttt tataaagcaa aatccatatt agattattca
     1321 cttgaaaatt aagtgattgg aggggagctt atgaaggata aaaaaagttt aattataatt
     1381 ttagctacgt taatagttgt cattatagga ggcatttatt atatgaaaac taacaagtca
     1441 aataaagttt caggatttgt tcaagaggaa tatagtataa aaaatacaac aaagaaaaat
     1501 tttttaatta agataaaaga aggatattca gaagaaatta taaatatgaa tttaatggat
     1561 aattttataa aagctgtaga tagtaaaaaa aatgatgaga tttatatcat agagtatgaa
     1621 agaaatgaga atggggagat tgttacatca ttaaaaaaat taaattatga cggaagtgaa
     1681 attaaagttt tagaatatga tgtttcaaat caaaaggagt tcaaagaagg tgagcttata
     1741 gcatataaaa aaataactaa aactggagat aattttggag caagaatagt tggaacaaca
     1801 agtgaagttc aaccaataat ggaaggagac atattatttg aatatgttga aagtaataag
     1861 gttgaatatg aataaaacta aatataaagg ttaatggtca agaatattaa ggaagttctt
     1921 aaatttaaga agtttctttt taatatatgt aaagattcaa ggggggatca tcttttatga
     1981 ataacaaatt gaaaaaaaac ctatcaaaag ttatgacagt tgcactgtta gcatcggtga
     2041 cagtaggttc aattccaagt atggcaaagg ctgaagaagt tgaaaaactt ttaaatatgc
     2101 cattaacagt agaaagcaaa gaacaaataa ttaacaactt taaaaatgat ttaataaagt
     2161 cagacactat aatcaaagat aatcaaaatc aggaagaagt agttagagta attgttgaag
     2221 taaacggaaa gtcagcacaa gaaatgattc ctagaggatt aagaccaaca gaaaaagaat
     2281 ctagtcaggt agaagcagct caaaaacctg ttagagatga agttgcaact atagaaggcg
     2341 tagaggtaag acatagttat actaatgtat ttaatggttt tagtgctgag gtaaaaagaa
     2401 aggatataaa acaaatagag gaagtgaatg gagtaagaaa agttacagaa gcacaaaaat
     2461 atactgaaga tatgaatagt gcaaagaaac ttactcaaat cgaagatgta tggaaaaaat
     2521 atagtttaaa aggtgaaggt atggttgtag ctatactaga tactggaata gattataagc
     2581 ataaggattt taaaaatcca gaggatactg gtaagttgaa gttaaagaaa gatgaagttg
     2641 aaaaagttaa ggcaagtgga gttttaaagg ctgacaagaa tgctaatact tattttactg
     2701 aaaaaattcc ttttggatat aattatgctg atagaaatga acaaatagta gatttaaggg
     2761 attcaaaagg acctcacggt gctcacgttg ctggcatagt tggagctgat ggagatgata
     2821 agttaattga tactaatgaa tcagtaaaag gagttgcacc agaagttcaa ttattagcta
     2881 tgaaaatatt ttctaatgga ccacttggaa aatatactta ttcagatgac cagttagcag
     2941 ctatagatga ttcagttaca ttaggtgccg atgttataaa tatgagttta ggttctccag
     3001 caggatatag agaggattca gacccagttc aagatgcaat tacaagggct acaaattcag
     3061 gggtaatggt tgtagtttca gcaggaaata gttctgcatc aacagatcct tatggacttg
     3121 gtatgttaaa tgaccaaata acagtagggg ctcctgcgtt agctaaggat gcgttaatgg
     3181 ttgcaagtta tgaaaatact actgttgcag aaaaagttat tgtatttaaa gatggtaaag
     3241 gaaaagttgt aactgaagga agttttaaag atcaccaagt tgattataaa gatatgttaa
     3301 ataaaaatta taaaatagtt gattgtggat tgggaaaagc agaagaatgt aaggatgtta
     3361 aaggaaatat tgctcttata aaaagaggag gacttacatt tatcgaaaaa ataattaatg
     3421 ctcaaaataa tggtgcagtt ggtgtagtta tatataataa agacggtgat gaatcactta
     3481 taagtatggc tacagatcca aatattaaaa taccagctat atgtgtagga aatactactg
     3541 gtaagaattt attaaatgct atgaaaaaag atacagttct atttaatgga gatttgaaaa
     3601 atgctacaag cgaaaatagt aacagctctg atttttcaga tttcacttct tggggtccat
     3661 ctcctagttt agaatttaaa cctcaagtag cagcaccagg tggacaaatt ttatcaactt
     3721 taaatgaagg tgaacatggg gttatgagtg gaacatctat ggcagcgcca catgcagcag
     3781 gttcaatggc tttattaatg gaagctatta aagaatatgc accggaactt aaaggtagag
     3841 aactaataga ttatgctaaa aatataatga tgaatacttc aagtgttaaa atagataaat
     3901 ttgctaaaaa tattccttat tctccaagaa gacaaggtgc aggacttgtt caagttgaag
     3961 atgctataag aaatagagtt accgtaacaa ataatgggga agcatccgtt gcacttaaag
     4021 aaataagagg agataaagct acatttactt tagatttaaa aaactatggc agtgaagata
     4081 tagaatataa agttgaagag ttaggtggag ttttaactca agatgaaaac actgaatatg
     4141 gtgaaatggt aagagataaa gctttaaata aaaaagatgc tagtttagaa tttagtagtg
     4201 ataaaattaa agttccagct aagggaaatg ctaaagtaga tgtaacatta aaaataggta
     4261 gtggattatc agaagataga tacttagaag gattcattaa atttacacct aagaatgaaa
     4321 aagtgccaac attggcaata ccatatttag gatattatgg tgactggggt aaggaaagta
     4381 taacaactaa taatgcatgg gatgaagata agcatatatt agttgaaaca ctaaaatcaa
     4441 ctggggaata tccaggtgta ttagttgaaa acttagctgt tactaaagtt aatgcagaag
     4501 attctataat gggtgttgta ggtaagaatg aagataaaag taataaatat gatagttcta
     4561 aaatagctat aagccctaat ggcgattcta tgaatgatac aatttatcca ggattatatt
     4621 tgatgagaaa tgctaaaaca gcatcagtag aaatagtaga tgaaaatgga aaattagtta
     4681 gaaatttagg aactacagaa aattatagaa agaaaatcat tagttctgaa tcaggaacaa
     4741 taccaatgat agctgatgat ttagcttggg atggtaaagt gtttgacaaa tctaaaggta
     4801 gttatgtaaa tgtttcagac ggaaactaca cttataaagt taaattaaaa gtagattatg
     4861 agagtgcaca ggagcaagta atagaaatgc cagttaaagt tgatacagtt ggaccaaatg
     4921 ttgaagttgt aaaatatgaa aatttagaag atggtgctat aaaactttac tttaaagcta
     4981 atgatgagtt atctggagta aatgaaaagg gtgaattccc tgtagttata aatggggcat
     5041 taaatgttga agctactaaa gcagcagtaa cttacgatag taagacaggg ctttatagca
     5101 agactataac tggtttacaa gctaatactt taaatcaaat agaaataggt gcatttgatt
     5161 atgcaaataa tttaggggga gtttcaactg ttataccagt aggaaaaata ccaccagcat
     5221 caataacatt tgatgataaa gtatttagtc aaggtcaaat tactgtgaat caaaatgaat
     5281 atactataag tggtaaaata aatagaccaa taaaagactt aattataaat ggagaaaaaa
     5341 ttaatttagc tataaataat gatggaacag ttaatttctc taaatcatta caattaagtg
     5401 aaggaataaa tgtagtaaat attaaagcgg tagattataa tggcaaggtt ttacaagatc
     5461 atgcatataa aattagctgt gatactatta aaccagtaat agaaatagaa agtccagaag
     5521 tagtagataa taagataact gcaactagtg atgttgttac attaaaagga agggtttatg
     5581 ataatctatg gggttacaag ttctatgtaa atggtgaact tattaaagta tcagattatc
     5641 caacacctgt tggaccagaa gtaaatggat atgacttctc agtagatgtt aaagatgtta
     5701 aaaatgggga ctttatattg ctaaaagctg tagatagtaa tggaaatgaa acagaattaa
     5761 aattacaagt agttagataa taaaaagagc ccttcggggc tttttttagt ggaaagagtt
     5821 cataagttaa gagttaatgt ttttaatttt atatcaaaat attaactaaa caaataaagt
     5881 aatgtaaaaa ataatggtat tatagtaaaa tataattatg tgtaaaaaaa tactgtacct
     5941 acaattgata aagtaggaat ttaaatatat ataaattagc attactatag taaaggagaa
     6001 aaacaatgat agttttaagt tgtaataaga ttcataagag ttttggaata gatgttattt
     6061 taaaagacat tacttttagt attaatgaag gagaaaagat agggcttata ggtgctaacg
     6121 gagcaggtaa gtctacgttg tttaaaatat taacttcaca gatggaatat gatagtggag
     6181 agctttttat agataaaagc aaaaaggtag ggtatttatc tcagaatcta tcattagagt
     6241 cagacaatac tatatatggt gaactactat tagtctttga agaactttta aatatggaaa
     6301 gaaaaatgaa gacattagaa gaaaagatga atgaaccata tgatgaagct aaagctgact
     6361 atcataataa attaataaag gattatacaa ccttaacaga attatatcaa catagaggtg
     6421 gatatactta taaaggtgaa ataagcagag ttttgaaagg attaggattt tttgaagaag
     6481 attttgaaaa gccaataagc atattaagtg gtggtcaaaa gactagagta gccctttgta
     6541 agcttttact tagaaagcca gaaatattgc ttttagatga gccaacaaat catttagatt
     6601 tagatgctat agaatggtta gaagaatatt taaagagcta taaaggtagc attattataa
     6661 tttcccatga tagatttttc ttagatgcag ttacaaataa aactatagaa atatcatatg
     6721 gtatggctga aatatataat ggtaattata cagcatttat tgacttgaaa aagaaaaatt
     6781 acgaagtagc attaaaggca tttagtttgc aacaagcaga aattaaaaga caagaagaga
     6841 taattcaaaa gtataagtct tttaaccgtg aaaaaagtat taaggcagca gaaagtagac
     6901 aaaaagcatt agataaaatt gaaagaatag aagcaccaca taaagaccaa aaggcatcta
     6961 gaattaattt tgaaactaga ataaaaagtg gaaatgatgt tttatatgtt gaagaattaa
     7021 gtaaaagtta tggggaaaaa gtattattta caggcgttaa tatggatata aaaagagaaa
     7081 ataaaattgc gcttataggt gaaaatggta gaggaaaaac tacattattt aaaataatta
     7141 tggatgaaat acaaccagat agtggaatca aagtattagg caaaaatgta aacataggtt
     7201 attacgatca ggaacaatct aatttagatt tgaataaaac aataatagat gaggtttggg
     7261 atgattttcc agaattaact accacggaaa taagaacata tcttgcagca tttctattta
     7321 gaggtgatga tgtgtttaaa aaaattgaaa agttgagtgg aggagaaaaa tgtagaataa
     7381 atcttctaaa acttatgtta tcgcaaaata attttttact tctagatgag cctacaaacc
     7441 atttagatat agtttcaaga gaagctttag aggatgctat attatcttat gatggaacag
     7501 tacttgtaat atctcacgat agatatttct taaataaagt agtaggtaaa atttttgaac
     7561 taaatgaaga tggaataaaa gaatatttag gaaattattc ttattatata gagaagaaaa
     7621 acaacccttt aagatttgaa caagaagaac aaagtagtgg gattacgaaa actcaaataa
     7681 aattagataa gaagaaaaaa agggaagaag agaaaaaaca aagagaaatt caaaagtatt
     7741 taaaaaatct tgaagaaaac atagcaacaa cagaaagtga aatagaaaag cttcaagaat
     7801 tactttgtaa agaagaaata tattctaatc cagaaaaaag tgtagaggta aatgaattat
     7861 taagtgaaaa ggaagaatta ctatctaact tatacgaaga atgggaagag aatttataag
     7921 aatttaagaa gaaactggat tagtccagtt tcttttaact ttaaattaat acaaattatt
     7981 tatataaaaa tatacttaaa tttataaatt atataataaa taggtaatat aacaactatt
     8041 tgctatactt atagtaatac taaaagtttt tttatatctt aaaaccaata atgtttatat
     8101 ttcttctaat ttgtataaaa aaataaaaat ttattatcta attaatgatg taatgaacaa
     8161 tttaaataaa tgtataaata aatgaaaaat aggtatagtt aaggtatatt tgtatttaga
     8221 aacatatatt tatagtacat gtttcatttg ttttaaaatt aaaattttac acatataaat
     8281 tagtttaata aataacaaaa aaattaagat ttatatataa atgtttaaat ttattgaaaa
     8341 aacaaggtga atattaagaa aatagagaaa agaatgaaac aattttgaaa atatatataa
     8401 acaaatttca agtaatatgg taaaatataa acgatatttt attaattata gtctttctat
     8461 ataaattttt agatttaatt atatttaaat tatatgtatt attaagtatg tatacgataa
     8521 ttttgaaatg aaacttacaa ttgaaaaata aaaaatatta ttttacataa tatatgaata
     8581 aagtaactct gtaattctgt aaaataaatt ttagttgatg aataattgag ttggaatatt
     8641 tataaaaatt cagaattaag cttattaata aaatatagga aagttttaat ttatatatat
     8701 ttttagattt tagatattat caagtgtatg cttacttgaa aagtgtccaa aattttcaat
     8761 gaataattta aaaggttatt ttggaatcct ataactaggt ttctaaaatt taaaatgtat
     8821 aaataaaaat attgttttga aacctatagt tagatttagt tattataaat tcaatttaaa
     8881 aataaaaagg aaaaagtctt tgtgattagg tacaaagatt tgaaataatc atatatgatt
     8941 attttttaga tttacctttt aaaaatatat ttttggaggt gttttttttg gatagaaaga
     9001 aaaatatatc aatggcagtt ataaagagaa ttcctaaata ttatagatat ttaaaagaac
     9061 ttgcagaaaa tgatgtggat agaatatcat ctaatgaatt aagtgaaaaa ataggcttta
     9121 cagcttcaca aataagacaa gatttaaatt gttttggtga ctttggtcaa cagggatatg
     9181 gatataatgt aaaagaatta ttagctcaaa tatcttcaat tttaggatta gataaagagt
     9241 acaatacaat aattataggt gcaggtaata tcgggcaagc tatagcaaat tatacttatt
     9301 ttcaaagaat gtcctttgat ttaaaggcaa tatttgatat aaatcctaaa cttattggct
     9361 taagaataag agatgtatct atagaagata tagataatct agaaagcttt attaaaaata
     9421 atgaagtaga tattgcaata atttgtgttc caaaagaaag agcacaagga gtttgcgata
     9481 ttataacatc atcaggaatt aaagctatat ggaattttgc gccagtagat ttaaaagttc
     9541 caagtgatgt aatagtagaa aatgttcatt taagcgaaag tttaatgact ttaagttatt
     9601 tactaaatga aagtaaatta actgaatagc ttttgtgaaa aggtcatcaa aaatagaaaa
     9661 taaaattgcg atgatctttt tcactttttt gtagaaaaaa agagatttaa ggataaaaaa
     9721 cagaagtaaa tattttgtta acaaatacac aaaatgttta aaaaatgaaa gaattttaat
     9781 acataataaa aaaaataaat catacattat ttagtgagat gtaaagaaaa tagggtttta
     9841 tgaaactttt ctatactaat cttagttaaa gaatatagtg acaaagcctg tagggtcttt
     9901 acattgttaa ataaaaacac aaattgtttt aattttacaa acaaatgaaa ttactaaaat
     9961 taataattaa ttaaaataat tcagaaaaaa tcatcaactt atgttgcaaa tatataccag
    10021 tgaggatata atagtaattg aagttgatgg acttcaatta ttatttttag taaaaattgt
    10081 gaattatata acaatttgtg ggaaaatatt catgggggac agcattatgg aatttaaaaa
    10141 tattatcctt gaaaaggatg gaaacatagg tattgtaaaa tttaatagac ctaaggcatt
    10201 gaatgcttta aattcagaaa ctttaaaaga gttggacaat gtcattgatt taattgaaaa
    10261 agacaatgag atttatgctg ttattttaac tggggaaggt aaagctttta ttgcaggagc
    10321 tgatatttca gaaatggaag gattgaatgc attagaagga agagcctttg gaatacttgg
    10381 aaacaaagtt ttcagaagat tagaaacttt ggacaaacct gttattgctg ctattaacgg
    10441 ttttgctcta ggtggaggtt gcgaactttc tatggcttgt gatattagaa tcgcatcagc
    10501 aaaagctaag tttggacaac cagaagttgg acttggaata acaccaggtt ttggtggaac
    10561 acaacgatta gctagacttg tgggagagtg taaagctaaa gagttgatat tcacagctaa
    10621 cataataaaa gcagaagaag catttaatat aggccttata aataaggttt tagaaccaga
    10681 agttttaatg gaagaagcta tgaatatggc aaaacaaata tgcaataatg caccagttgc
    10741 agttaaactt tgtaaggaag caataaacaa aggtatgcaa atggacattg atagtgcatt
    10801 aaattatgaa tcagaagctt ttggtatgtg ttttagtact tatgaccaaa aagaaggtat
    10861 gaaagcgttt ttagagaaaa gggataaaaa ctttaaaaat atgtaatcct ttaacttatt
    10921 ttaatacgta attgtaatgg aaaatcaagt ttgaaatttg aaaaaataag aaaattagcc
    10981 ttttaaaaat taaataggag gaatggggag tatgaatttt tcattaacta aggaacaaga
    11041 atttgtaaga caaatggtaa gggagtttgc agaaacagat gtaaaaccta ttgccgctga
    11101 aatagatata acagaagaat ttccaatgga aaatgtaaag aaaatggcaa gatacaatat
    11161 gatgggtatt ccattttcaa ctgaatttgg aggagcaggt ggaaacaacg tttcttatgc
    11221 tatggctgtt gaagaattat caagagtttg tggtacaaca ggcgttattt tatcagcaca
    11281 cacttcatta tgtgcatctc caatagatat gttcggaaat caagaacaaa aagaaaaata
    11341 cttaacacca cttgcaaaag gtgaaaaaat aggagcattt ggtttaactg aacctaacgc
    11401 aggaacagat gcatcaggac aacaaacaac tgctgtttta gatggagata actatatatt
    11461 aaatggctct aaaatattca taactaacgg tggagttgca gatgtattta tagtatttgc
    11521 tatgacagat aaatctaaag gaacaagagg aatttcagca tttatagttg aaaaaggttt
    11581 taaaggattt tcaataggaa aagttgaaga aaaattaggt ataagagctt cttcaacaac
    11641 agaattagta tttgaagatt gtatagttcc aaaagaaaac ttacttggaa aagaaggcca
    11701 aggtttcaaa atcgcaatga aaacattgga tggaggaaga attggtatag ctgctcaagc
    11761 tttaggaatt gcacaaggtg ctttagatga agctgtagct tacatgaaag aaagaaaaca
    11821 attcggaaga tcattatcta aattccaagg tcttcaatgg atgatagcag atatggatgt
    11881 tcaagtagaa gctgctagac aattagttta taaagctgct tacaacaaag ataataagct
    11941 accatataca gtagaagctg caagagctaa attatttgct gctaatacag ctatggatgt
    12001 tacaactaaa gctgttcaaa tctttggtgg atacggatat actaaagatt atcctgtaga
    12061 aagaatgatg agagatgcta aaataacaga aatatatgaa ggtacttctc aagttcaaaa
    12121 aatggttatt gcaggaagtc ttttaaaata gggggtaatt aacgatgaaa atagttgttt
    12181 gtataaaaca agttccagat acaactgaag ttagaataga tccaaaaaca ggaactctaa
    12241 taagagatgg tgttccttca ataataaatc cagatgataa aagtgcatta gaagaagcat
    12301 taagattaaa agattcacat ggtgctcatg taactgttgt tacaatggga cctccacaag
    12361 ctcaaaaagc tttagaagaa gctttagcaa tgggagctga tgaagcaata ttagtaacag
    12421 acagagcttt cgctggtgct gatactttag ctacatcaca tgcattagct ggagtaatca
    12481 aaaaattaga atatgatgta atatttgctg gaagacaagc aatcgatgga gatactgcac
    12541 aagttggacc tgaaattgca gaacatttaa acttaccaca aataacatat gttgaagaag
    12601 ttgacgtttt agaagacgga ttaaaagtta gaagagcttg ggaagatgga tatgaatata
    12661 tcaaagttaa aacaccagtt ctattaactg caatagaaac tttaaatgaa ccaagatata
    12721 tgaatataag aaatatattc gaattaggaa ataaagaaat aaaagtatgg actgctaaag
    12781 atatagatgt agatgtagcc cttctaggat taaaaggttc acctacaaag gttaaaaaat
    12841 cttgggctaa agaagctaaa ggacaaggtg aaatatttac agtttcacca aaagaagcag
    12901 cttcaattgc aataggtaaa ctaaaagaga aacacttaat ttagtaggaa aaataggagg
    12961 ggtagaacca tgaatatagc agattacaaa ggtgtttggg tatttgcaga gcaaagagac
    13021 ggagaattac aaaaagtttc attagaactt ttaggaaaag gtagagaaat agctgacaaa
    13081 ttaggagttg aacttacagc tttattaata ggacataatg ttgaagatat ggctaaagaa
    13141 ttaactgctt acggcgcaga taaagtttta ttagctgata aagaagaact tgcacattat
    13201 actactgatg gatatgcaaa agttataact gatttagcta atagtcataa accagaagta
    13261 atattcatag gagcaacatt tataggaaga gacttaggtc caagagttgc tgctagatta
    13321 tcaacaggat taactgctga ctgtacagca cttgatatag atcctgaaaa caaaaactta
    13381 ttaatgacta gacctgcatt tggtggtaac ttaatggcta ctatcatgtg tggagacaat
    13441 agaccacaaa tgtctactgt tagacctgga gtttttgaaa aacttgaaaa agatacaaac
    13501 aaagaagtta aaatagaaaa agctgttttt gaattagaaa aatctgatat cagaactcaa
    13561 gttttagaag ttaaaaaaat attaaaagaa gtagctgaca taatcgaagc tgatatatta
    13621 gtatctggtg gtagaggaat aggttgcaaa gaaaactttg atatattaaa agaacttgct
    13681 gatgaaatcg gcggaacttt atcaggatca agagcagctg ttgataatgg ctggatagat
    13741 aaagcactac aagttggaca aactggtaaa acagtaagac ctcaaatata tatagcttgt
    13801 ggtatatctg gagctatcca acatttagct ggaatgcaag atagtaacta tataatagca
    13861 atcaataaag atgctgatgc accaataatg caagttgctg atgttggatt agttggagac
    13921 tatcaaaaaa ttgttccaga attagttcaa caattaaaag ctatgaaata ataagtggtt
    13981 aatataacac ttttataata accaaataag atgtgaattt aggaattatt cctaaattcc
    14041 atctttaaaa attaaacata tgaaaaacat aggggaagaa actttaattt aaatttatct
    14101 aatgaattta aaatgtaaaa acatatataa tttttataaa ttaattaaaa ataaggaata
    14161 aacattaggt gatatataca attcttataa agagttgtaa taatattttc aaaagattat
    14221 ataaatagta atgaacatgg tagttaataa tagataatta ataagttaat aatacaaaaa
    14281 ttatttaaaa agtatcaaaa attttatttt tgattatcga ttattaatta tcattaaatt
    14341 ttatctatga tagttaaaaa taatacaaac ttaaaaaaat aaggtttaag ttagttgttt
    14401 tatatatcgg gcacttataa gctattggat ttttaagtgg gtcatatatt taaacagctt
    14461 caactaaaaa ttggggggtt ttgttatgaa aaaaatatgt ataatcggtg ccggaactat
    14521 gggagctgga atagctcaag tacttgctac aaaaggttat gaagttacaa tgcttgtaag
    14581 aaatatggat aaattccaaa atgtaaattt agcaggtata aataaaactt taactaaatt
    14641 agtaactaaa ggtaaaatta cagaagacgt aaaaggtgaa attttaggaa gaatcaacgg
    14701 aacattagat atgaatgatg ttaaagatgt tgatttaatt attgaagcaa tagctgaaaa
    14761 tatggaaaca aaaagacatt atttcagtaa attagatgaa atctgtaaac cagaaacaat
    14821 atttgctaca aatacttctt cactttcaat aacagaagta gctagctgca caaacagacc
    14881 agatagagtt ataggaatgc acttctttaa cccagcagct ataatgaaat tagttgaaat
    14941 tataagaggt atggctactt cagatgaaac atttagcgca gttaaagaat taacacaaag
    15001 tataggaaaa gaccctgtag aagtaaaaga aggtccagga ttcgtagtaa atagaatatt
    15061 aatcccaatg ataaatgaag cagttggaat attagatcaa ggtatagcaa ctgtagaaga
    15121 tatagacaaa gcaatgatgc ttggagcaaa ccatccaatg ggacctttag cattaggtga
    15181 cttaatagga cttgatatat gtcttgcaat aatggaagtt atgtataaag aaactggaga
    15241 ctctaaatac agagcaaaca gtttattaag aaaatatgtt agagctggat accttggaag
    15301 aaaaactaaa aaaggtttct atgactataa caaataaaat tgatataaat ataaagagct
    15361 tgagaacacc tcaagttctt tttttatgta aaaatgttca ttgtaaacaa ggttaagaca
    15421 attaattagg aagtgataat ggacaattaa aaaaataagg atttaggagg aaactagagt
    15481 ttagttagtt aagagttaag agttcaggtt gaaactggac tgccagtttc ttttgaatta
    15541 gaaggagtaa attttgagtt taggatataa aacaaactaa agctagattc aaagtgtagc
    15601 gccgtaacaa atgggacagg cacgaatggt ttagcgttat aaaatgaaaa tcagctctta
    15661 aaatttagtt acaataagta agaaaagtta gccagtccct tataaatagt taagagttaa
    15721 aaattaacag ttaagagttt aggaggaaag tggcaatcac tttctttgaa attgttcggg
    15781 actgactttg gtgttttaat aagtagcata aatttaggag agctttagat agttaagagt
    15841 taaaagttaa gagttgagga tgaaagtgga aaccactttc tttggaattg ctggggactg
    15901 gctttgattt tttagttgaa aaatataaaa cgaggagagc atcaaatgtt agggaagaaa
    15961 ataaatttat cgtgcctgtc ccatttgtga cggagctaat atgaggagta attgcttgtt
    16021 taggtgtttg ggaccgactt tgagtactat tggagagaaa gtaaaatcaa caaaagcatc
    16081 tatcccaaag tatagcaaag tagccaacta cgcgtctgtc cctcttgtaa tggaggtaga
    16141 actttgggtg attgcttgct tagttgagat aagtagttta aaaaattgac aaaggacaat
    16201 tgacatttga caatgatgga ggaaactgga ctaccagttt ttgaatttag gggaataaat
    16261 tctaggttag agagattaaa ctaaacaaaa gctatattca aagtgcacag ccgtaacaag
    16321 aaggacaggc gtacctaagt atattttcca taagttattt tgtgagtgaa atcataaagt
    16381 tatcaataaa aattaaagta atcaaagcct gtccttaaga tagttgatag ttaaaaatta
    16441 atagttaaaa gtttaggatg aaagtggaaa ccactttctt tggaattgct ggggactggc
    16501 tttgattttt tagttgaaaa atataaaacg aggagagcat caaatgttag ggaagaaaat
    16561 aaatttatcg tgcctgtccc atttgtgatg gagctaatat gaggagtaat tgcttgttta
    16621 ggtgtttggg accgactttg agtactattg gagaaaaact aaaatcaaca aaagcatcta
    16681 tcccaaagta tagcaaagta gccaactacg cgtctgtccc tcttgtgatg gaggtagaac
    16741 tttgggtgat tgcttactta gttgaggtag ggcgcttgaa aaattgacaa aggacaattg
    16801 acatttgaca atgatggagg aaactggact acccgttttt gaatttaagg gaataaattc
    16861 taggttagag agattaaacc aaacaaaagc tatattcaaa gtgcacagcc gtaacaagaa
    16921 ggacaggcgt acctaagtat attttccata agttattttt tcggtgaaat cgaaaggtta
    16981 tcaataaaat ctaaagcaat caaagccggt ccttaagata gttaatagtt aagaattaat
    17041 agttaagagc ctatagcccc cctaattgga ttggacaaag ttaatttaga tatttacaat
    17101 tatattaatg aaattaggag ggctacaata tgtcaaaagt tgtttttaaa agaaagtttt
    17161 cagaagaa
//
