LOCUS       c00043_NZ_JALC..       15086 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JALCKS010000043.1 MAG: Aneurinibacillus aneurinilyticus isolate
            UW_TS_ANEURI1_1 Ga0455822_00231, whole genome shotgun sequence.
ACCESSION   c00043_NZ_JALC..
VERSION     c00043_NZ_JALC..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:27:27
            Original ID  :: NZ_JALCKS010000043.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15086
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15086
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[312:5086]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      313..5086
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..15086
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15086
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             3..149
                     /locus_tag="ctg43_1"
                     /transl_table=11
                     /translation="EQALAVNPTTIGSACPYCLTMLSDGTKAKEVEEKVQTLDMVEILE
                     KSL"
     CDS             313..1500
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_2"
                     /sec_met_domain="Thiolase_N (E-value: 3.9e-105, bitscore:
                     342.2, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 5.2e-47, bitscore:
                     150.0, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKSVIVSAARTPFGKFGGKLSSLTAVDLGGVVIKEAVERAGISP
                     DMVEEVIMGMVLQGGARQIPSRQAARKAGLPWEVQTETINKVCASGLRSVTLADQIIRA
                     GDADVIVAGGMESMSNAPYIVPNARWGMRMGDGKIVDLMLHDGLTDAYDGQHMAVHGSN
                     VAAEYEISREAQDKWALRSQQRAAEAIKQGKLKDEIVPVSVPQRKGEPVLVDTDEAPRE
                     TSMEQLQKLPPVFTKDGTITAGNAPGVNDGAGAMVVMSDEKAKELGAKPLATILGHAAV
                     GQEAPYIATTPGLAINKLLKKTGYTIDQIDLFEVNEAFAAVTLTSEKIAGWDPEKVNVN
                     GGAIAFGHPIGASGARILMTLIYELRRRGGGLGIAAICSGAAQGDAILVEVPAAE"
     CDS             1767..2618
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_3"
                     /sec_met_domain="HCDH (E-value: 1.8e-40, bitscore: 129.0,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 2.3e-68, bitscore: 221.0,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVKKFMVIGAGQMGSGIAQVAAQAGLHVVLHDVKEEFVNRGLGV
                     ITKNLTRDVEKGRKSEQEKQAILERIVVSTDINSAKDVDFVVEAVVENMDVKTAIFKQL
                     DDITPAHTILASNTSSLPITEIAAVTNRPEKVIGMHFMNPVPVMKLIEVIRGLATSDET
                     YRIVADLSEKMSKVAVEVNDFPGFVSNRVLMPMINEAIYCVYEGVATPESIDEVMKLGM
                     NHPMGPLTLADFIGLDTCLYIMEVLHEGFGDSKYRPCPLLRKYVKAGWLGRKSGRGFYV
                     YE"
     CDS             2651..3790
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_4"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 5.8e-55, bitscore:
                     177.0, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 8.7e-32, bitscore:
                     100.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 7.7e-40, bitscore:
                     127.5, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLQFTEEQEMMRRMVRDFAREEIAPQVEKMEETDEFPIEIIRKM
                     GEYGLMGIPIEEKLGGAEADFISYILAIHEISKVSPTVGVILSVHTSVGTNPIVYFGNE
                     EQKKKYVPKLASGEYIGAFALTEPNAGSDAASLRTAAVRKGDKYILNGSKVFITNAGAA
                     DTYITFAVTDPSKGPKGISAFIVEKDTPGFTVGKKEKKMGLHGSNTSELIFDNAEVPAE
                     NLLGEEGMGFTIAMANLDIGRIGIAAQGLGIAEGAFEHALAYAKERHQFGKPIAAQQAI
                     AFKLADMATQIEASRLLVYRAAALRGQGVTCGMEASMAKRFATDTAMNVATEAVQVFGG
                     YGYTREYPVERYFRDAKVTQIYEGTNQIQRIVISKHLIK"
     CDS             3944..5086
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_5"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 4.1e-54, bitscore:
                     174.2, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.4e-31, bitscore:
                     100.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.7e-43, bitscore:
                     138.7, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFQLSEEHKMLRQMVREFAENELADKAGERDEEERFDMEVFHKL
                     AELGLTGIPWDEKYGGAGFDFLSYVIAVEELSRVDSSVGVTLSAHTSLASWPIYKFGTE
                     EQKQKFLRPLAEGKSMGAYCLTEPGSGSDAAGMKTTAKKDGDHYILNGSKIFITNGGLA
                     DIYIVFAATTPELKHKGQTAFIVTSDMPGFKVGKKEKKLGIRSSATTEIIFEDCRVPVE
                     NMLGTEGQGFKIAMMTLDGGRNGIAAQAVGIADGALTHAIAYAKERHQFGKPIAAQQAI
                     GFKLADMATKVEAARLLTYQAAVLEDSALPYGKASAMAKLYAGDIAMEVTTDAVQVFGG
                     YGYTREYPVERFMRDAKITQIYEGTNEIQRLVISNYLLKD"
     CDS             5100..6044
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MeaB"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg43_6"
                     /sec_met_domain="MeaB (E-value: 2.2e-96, bitscore: 313.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LHALVERILNGDRRAVARAISYIEDNHPEKTEILQDLFPHTGGAF
                     LLGITGSPGAGKSSFVDKLIGYLRKQGLTIGIVAVDPTSPFTGGAILGDRIRMQDHFLD
                     EGVFIRSMGTRGSLGGLARATKEAVRVLDAYGKDIVIIETVGVGQSELDIMNIADSTAV
                     VLNPGGGDTVQAFKAGIMEIADLFILNKADLAGTDKLFREVEQMLDLVKHDSPWRPPVV
                     KTITTQGMGIDEAWQAFLDHKSYLQESKEWERRRASHLREEVSEIVEHAVHQLIVKRLR
                     SEEYKDMMEGVSRRDIDPYEVAHQLLQQLVKEK"
     CDS             6180..6806
                     /locus_tag="ctg43_7"
                     /transl_table=11
                     /translation="MEQRKSIPSMVKDPKLIEKRREQIIEAAVELFISKGFHKTTTREI
                     SRASGFSIGTLYEYIESKEDVLYLVCDAIHAGVEKGLQEVLVNGVSGAVMLEAAIEGFL
                     RVMDQMQDRVLLIYQEIKSLPRETMRYVLAKEEEITGFFEEILHRGRRDGSLQIDENSV
                     KLMAHNIVVLGEMWTFRRWSLRKNYTLDEFTKMQTSLILRELTDK"
     CDS             6918..10172
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) B12-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MeaB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MM_CoA_mutase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg43_8"
                     /sec_met_domain="B12-binding (E-value: 1.4e-16, bitscore:
                     52.1, seeds: 91, tool: rule-based-clusters)"
                     /sec_met_domain="MeaB (E-value: 2.8e-44, bitscore: 142.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="MM_CoA_mutase (E-value: 1.1e-146,
                     bitscore: 480.7, seeds: 314, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTIYRPKHNVRFVTTASLFDGHDASINIMRRILQASGVEVIHL
                     GHNRSVEEIVNAAIQEDAHGIAISSYQGGHVEYFKYIVDLLKEKNASHIKVFGGGGGVI
                     VPSEIRELHEYGVAKIFSPDDGRVHGLQGMINSMIEQTDYPTPKEVEKEISELKSRSWN
                     AIGRMITLAEENVERQDREVAATLEKVNAMVETVPVLGITGTGGAGKSSLTDELVRRFL
                     EQYPEKTMAILSVDPSKQKTGGALLGDRIRMNAINSPRVYMRSLATRKSHSELSKAIGD
                     AINIVKAAGYDFIIVETSGIGQGDAQITQVCDIAMYVMTSEFGAPTQLEKIDMIDYADI
                     IAINKFERRGSEDALRDVKKQWTRSRAIFDMKDEDIPVYGTIASQFNDPGTNVLFVNLM
                     RVVSEKTGTEWPTTLNISGNTKSKKQYVIPSERIHYLAEIVNTVHQYKKFTEQQVQIAR
                     KLFQLKGTKEELLAHTGELASDLQQEIDKLISHYEEKLHPESRKILDNWKSLKEAYMKD
                     EFITKVRDKEIVTKLYTTSLSGTRIPKVALPKFEEWGEILRWAMSENLPGYFPYTAGVF
                     PFKRDGEDPKRQFAGEGTPERTNRRFHYLSKDDAAKRLSTAFDSVTLYGEDPDYRPDIY
                     GKIGTSGVSICTLDDMKKLYDGFDLCHPSTSVSMTINGPAPIILAMYMNAAIQQQIDKF
                     KQENGREPNAEEAKQIKAYTLKSVRGTVQADILKEDQGQNTCIFSTEFALKMMGDIQEY
                     FIENHVRNYYSVSISGYHIAEAGANPISQLAFTLANGFTYVEYYLSRGMHIDDFAPNLS
                     FFFSNGLDPEYTVLGRVARRIWATVMKNKYGGNERSQKLKYHIQTSGRSLHAQEMDFND
                     IRTTLQALMAIYDNCNSLHTNAYDEAITTPTENSVRRAMAIQMIITKELGLAKNENSLQ
                     GSFIIEELTELVEEAVLAEFQRISDRGGVLGAMETQYQRSKIQEESLYYETLKHNGELP
                     IIGVNTFLNPNSSEEDYEIELARATEEEKVQQINNLRDFQERHKDVADAALRRLKEVAR
                     SGENIFAELMETVKVASLGQITGALYEVGGQYRRNM"
     CDS             complement(10123..10251)
                     /locus_tag="ctg43_9"
                     /transl_table=11
                     /translation="MPSPFIFSSTEFLFGTKEFTIVSSFDPTYFDDIVHRLHRAHL"
     CDS             10432..10941
                     /locus_tag="ctg43_10"
                     /transl_table=11
                     /translation="LSNALNLEAEEVKEMAMVDIAYQMLNQSKKPAYYRDLYKEITAMK
                     NMSESEAMDMIAQVYTEINLDGRFICLGENLWGLKRWYLVETQEESAESGFRGKFALDD
                     DDDFEDDEELEEEYDEIDNAHVDDEDEEEIDDDFADDEKETDEEFDEELDDEELENEDE
                     DEDLDM"
     CDS             11085..12734
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg43_11"
                     /sec_met_domain="GATase (E-value: 4.2e-62, bitscore: 200.7,
                     seeds: 97, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LWKSIEWAKKEGFICMAKYIFVTGGVVSSLGKGITAASLGRLLKN
                     RGLQVTIQKFDPYINVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFIDINLNSNSNV
                     TTGKIYSSVITKERRGDYLGGTVQVIPHITNEIKERVFRAGRETNADVVITEIGGTVGD
                     IESLPFLEAIRQIKSDVGRDNVMYIHCTLVPYLAAAGEMKTKPTQHSVKELRSLGIQPN
                     VIVCRTEHPMSQDMKDKIALFCDIDPKAVIEMVDAESLYEVPLTLKEQGADEYVCNHLN
                     LTCPEADMTEWKQMLHKIKNLSHKTTIAIVGKYVALHDAYISVAEALYHAGYANDADVN
                     IEWVDAEEVYEHNVSELLSRVDGILVPGGFGDRGVEGKIIAAKYARENKIPFLGICLGM
                     QVAVIEFARHVVGMKDANSSEINPSTQYPVIDLLPDQKDIENLGGTMRLGLYPCKMEKG
                     TAAHTSYDEDLVYERHRHRYEFNNQYREALKDAGLKFSGTSPDGRLVEIVELPDHPWFV
                     ASQFHPEFASRPNRPQPLFRDFVGAALKLKKQ"
     CDS             12922..13287
                     /locus_tag="ctg43_12"
                     /transl_table=11
                     /translation="MKDKKVLIVDDQYGIRVLLFEVFGKEGYQTFQAANGKQALEIVTG
                     ESPDLVILDMKIPGMDGLEILKEIKKIAPETKVIMMTAYGELDMIKEATELGALTHFTK
                     PFDIDELRTAVNKQLAC"
     CDS             13351..14214
                     /locus_tag="ctg43_13"
                     /transl_table=11
                     /translation="MPLVSMTDMLNKAVEGKYAVGQFNLNNLEFTQAILQAAEEEKSPV
                     ILGVSEGAARYMGGFKLVVAIVQALMEEYKITVPVAIHLDHGSSFEKCVEAIHAGFTSV
                     MIDGSHYPLEENIALTKRVVDVAHALGVSVEAELGRIGGQEDDLIVQDAEAAYAIPAEC
                     DKLVRETGVDCFAPALGSVHGPYKGEPNLGFDRMEEVMKLTGVPLVLHGGTGIPTKDIQ
                     RAISLGTAKINVNTENQIASAKTVREVLDAKPDLYDPRKYLGPARDTIKETVKGKMREF
                     GSSNKA"
     CDS             14252..14899
                     /locus_tag="ctg43_14"
                     /transl_table=11
                     /translation="MRFFIDTANVNEIREVNEWGVLAGVTTNPSLVAKEGRDFVETLKE
                     IIDIVDGPISAEVISLEAEGMIEEGEKLASLSKNIVIKVPMTVEGLKAVKHFTKKKIKT
                     NVTLVFSATQALLAARAGATYVSPFLGRLDDISQDGLQLISDISQIFDAHDIETEIISA
                     SVRHPLHVVECAKMGADIATIPYKIFKQMAQHPLTDAGIERFLADWDQAQQK"
ORIGIN
        1 ccgagcaggc gttggctgta aatccgacga cgatcggaag cgcctgtccg tattgtctga
       61 cgatgctgtc agacggcacg aaagccaaag aagtggaaga aaaagtccaa acacttgata
      121 tggttgagat tttagaaaaa tctttataaa atactctttt tcatttgtaa gaacattgat
      181 acaatgaaag aggaaagaac tgttaacccg gcagttgtca ctgccgggcg tgtttcaagg
      241 tataaactga ctgagcaatc gttcaacttg tactgaagaa gtaagtcaaa tagataagga
      301 ggcgcgaaaa gaatgaaaaa atcggttatt gtaagcgctg cccgtacacc attcggcaag
      361 tttggaggca agttgtcttc acttacggca gtagatctgg gcggggtcgt aatcaaagag
      421 gctgtggaac gtgccggtat ttctccggat atggtggagg aagtcattat gggaatggta
      481 ctgcagggcg gtgccaggca gataccttcg cgtcaggcag cccgcaaagc agggctgccg
      541 tgggaggtac agacagaaac tattaacaag gtatgcgctt ccgggcttcg cagcgtgaca
      601 ttggccgatc agattattcg tgccggtgac gctgatgtta tcgtagctgg cgggatggaa
      661 agcatgagca atgcaccgta tattgtaccg aatgcacgct ggggcatgcg catgggcgat
      721 ggaaagattg tagatttaat gttgcatgat ggattaacgg atgcttatga tggccagcat
      781 atggcggtgc acggcagcaa cgtggcagcc gaatatgaaa tttcgcgcga agcacaagac
      841 aagtgggctt tgcggagtca acaacgtgca gcggaagcca taaaacaagg gaaattaaaa
      901 gacgaaattg ttcccgtatc cgtgccacaa cgcaagggag agccggtgct ggttgatacg
      961 gacgaagccc cgcgtgaaac aagtatggaa cagttgcaaa agctgcctcc tgtctttacg
     1021 aaggacggta cgattacagc aggcaatgcg ccgggtgtaa atgacggtgc aggggctatg
     1081 gtcgtcatgt cagatgaaaa ggcaaaagaa ttgggagcaa aaccgctggc aaccattctg
     1141 ggacatgcgg cagttggaca ggaagcgcct tatattgcca ctacgccagg ccttgctata
     1201 aacaagctgt tgaaaaaaac aggctatacg attgaccaga ttgatttatt tgaagtgaat
     1261 gaagcatttg cagcagtaac attaacgagt gagaagattg caggttggga cccggagaag
     1321 gtaaacgtca atggaggcgc tatcgcattc ggtcatccga ttggagccag tggcgcgcgc
     1381 atcctgatga cactgatata tgaactgcgt cgtcgcggcg gaggtcttgg cattgcggcg
     1441 atttgctcgg gagcagcaca gggcgatgct attcttgtgg aggttccggc agcggaataa
     1501 cgcgcagaag aagatagaga cgacctattc tcccattatt tatatagaaa tcacctgaca
     1561 tagtaacaaa agttcaggtg gttggaagga ggagattcgg aaaaaagaag agggtggatg
     1621 cgccccggca gaagaagggc caaggtgcct ttttccgacc gcccaccgcc cttccttttt
     1681 ttcttacctc ccaccacaaa catgtgtcga tttatcaaat cagatatata tccaggtttt
     1741 attaagaata aaaggagtgg agacgaatgg aagtcaaaaa attcatggta atcggcgcag
     1801 gtcagatggg aagcggcatt gcgcaggtgg cggcacaggc tggacttcat gttgtgctgc
     1861 atgatgtgaa agaagagttc gtaaatcggg gtcttggcgt tattactaaa aatctgacgc
     1921 gcgatgtgga gaaaggccgc aagtccgaac aagaaaagca ggccattctt gagcgcatcg
     1981 ttgtgtcaac ggatatcaac agcgctaaag acgtcgactt tgttgtagaa gcggttgtcg
     2041 agaatatgga tgtgaaaaca gccattttta agcagcttga tgatattaca ccggcgcata
     2101 ccattctcgc aagcaataca tcctcccttc cgattacgga aattgcagcg gtaacgaatc
     2161 gtccagagaa agtgattggc atgcacttta tgaatcctgt tccggttatg aagcttatcg
     2221 aagtgattcg cggtcttgca acatcggatg agacctaccg catcgtagcg gatctgtccg
     2281 agaagatgag caaagtagcg gtagaagtga atgatttccc aggcttcgta tccaaccgtg
     2341 tattgatgcc gatgattaat gaagccattt actgtgtata tgaaggtgtt gctacacctg
     2401 agtcgattga tgaagtaatg aagcttggta tgaaccatcc gatgggtcca ctgacattgg
     2461 ctgatttcat cgggcttgat acatgcctgt acatcatgga agtacttcat gaaggattcg
     2521 gtgattctaa atatcgccca tgtccattgc tgcgcaaata tgttaaagca ggatggcttg
     2581 gccgtaaatc cggacgcgga ttctacgtat acgaatagag ataaggcaac agaagatggg
     2641 aggagaaacc atgcatttac aatttacaga agaacaagaa atgatgcgcc gtatggtacg
     2701 ggattttgcc cgtgaggaaa tcgcacccca agtagagaag atggaagaga cggatgagtt
     2761 tcctattgag attattcgca agatgggtga atacggattg atggggattc ccatcgaaga
     2821 gaaattgggc ggcgcggaag cggactttat ctcgtatatt ctagctattc acgaaatttc
     2881 taaagtgagc ccgaccgtgg gggttattct ctctgtgcat acatcagttg gcacaaatcc
     2941 aattgtatat tttggtaatg aagaacaaaa gaaaaaatat gtaccaaaac ttgcttctgg
     3001 ggagtatatc ggagcttttg cactgacgga accgaatgcg gggtctgatg cggccagcct
     3061 gcgtactgcg gcggtgagaa aaggtgacaa gtatattttg aacggctcca aggttttcat
     3121 tacgaacgcg ggtgcagccg atacgtatat tacgtttgct gtcactgatc cgtcaaaagg
     3181 tccgaaagga atttcagcat ttattgtgga aaaggataca ccagggttta ctgtcggcaa
     3241 aaaggaaaag aaaatggggc tgcacggttc caatacatcg gaattgattt tcgataacgc
     3301 ggaagtgccc gcggagaatt tgctaggtga ggaaggcatg ggctttacta tcgcaatggc
     3361 aaacctggat attggacgca taggtattgc ggcgcaaggt cttggcattg ctgaaggagc
     3421 attcgagcat gcattagctt atgctaaaga acgtcatcag tttggcaagc cgattgcagc
     3481 gcagcaggct attgccttta agctggctga tatggcgact caaatcgaag cttctcgctt
     3541 gcttgtatat cgtgcggcgg cgcttcgcgg tcagggcgtt acgtgtggca tggaagcctc
     3601 catggcgaag cgatttgcga ccgacaccgc gatgaatgtg gcaacggaag cagtccaggt
     3661 attcggcggc tacggctata cgcgggagta tccggtggag cgttatttcc gtgatgctaa
     3721 agtaactcaa atctacgaag gtactaacca gattcagcgt attgtgattt ctaaacacct
     3781 aataaagtaa aactttcatc agtggaggat ctttcttttc cgctgatggc tagttgcact
     3841 tatcggggac ttaggggcag ttatccctcg tgcagcgcag atgaagtcag gaccttgctg
     3901 ccccttaaac cgagacaaac aaataaataa ggagtggaga gaaatggatt tccaactatc
     3961 tgaagagcac aaaatgctac gccaaatggt gcgtgaattc gcggagaatg agttggctga
     4021 caaagccggt gagcgtgacg aagaagaacg cttcgatatg gaagtgtttc ataagctagc
     4081 tgaattggga ttaacaggta ttccatggga tgagaaatat ggtggtgcag gcttcgactt
     4141 tctaagctac gtaatcgcgg tggaagaatt gtcccgtgtt gattcttcgg taggggttac
     4201 gctatctgct catacatcgc ttgcgagctg gccgatttac aaattcggta cagaggagca
     4261 aaagcagaag ttccttcgcc cgttggcgga agggaagagt atgggtgcat attgcctgac
     4321 cgagcctggc tctggatctg atgcggcagg catgaagacg actgcgaaaa aagatggtga
     4381 tcattatatt ctgaatggaa gcaaaatttt catcacgaat ggcggtctgg cggatattta
     4441 cattgtattt gccgcaacta cgccggagtt gaaacataaa ggacaaacgg catttatcgt
     4501 aacgagtgat atgccgggct ttaaagtcgg taaaaaggag aagaagcttg gtatccgttc
     4561 ttctgcgaca acagaaatta tttttgaaga ttgccgcgta ccggtagaga atatgctggg
     4621 cacagaaggc cagggcttta aaatcgctat gatgacgctg gacggtggcc gcaatggtat
     4681 cgctgcacag gcggttggaa ttgcagatgg agcattgacg catgcgattg cttatgcaaa
     4741 agaacgccat caattcggta aaccgattgc tgctcaacag gcgatcgggt ttaaactggc
     4801 tgatatggca actaaagtgg aagcggctcg tcttctgact tatcaggcgg ctgtgcttga
     4861 agacagcgct cttccatacg gcaaagcttc tgctatggca aagctgtatg caggcgatat
     4921 cgcgatggaa gtaacgactg atgcggtaca ggtattcggt ggttacggct atacgcgaga
     4981 atatccggtg gaacgcttca tgcgggacgc gaaaatcaca cagatttatg aagggacgaa
     5041 tgaaatccaa cgtcttgtca tctcgaacta tttgctgaaa gactaaggat gtgagcgtat
     5101 tgcatgcatt ggtagaacga attcttaatg gagatcgccg ggcagttgcc cgtgcgattt
     5161 cttatataga ggataatcat ccagagaaga cggagattct gcaggacctt ttccctcata
     5221 cgggaggagc gtttctcctc ggtatcaccg gctcaccggg tgcgggaaaa agctcgtttg
     5281 ttgataagct tattgggtat ctgcgtaagc agggattgac gatagggatt gtggcggtag
     5341 atccgaccag tccgtttacc ggaggggcca ttcttggaga ccgcatccgt atgcaggatc
     5401 actttttgga tgagggcgta tttatccgca gtatgggtac gcgcggaagt cttggcggcc
     5461 tggcacgagc aacgaaagag gcggttcgcg tacttgatgc atacggaaag gatattgtaa
     5521 ttattgaaac ggtaggtgtc ggtcaatcgg agcttgacat tatgaatatt gctgactcga
     5581 cagcggttgt acttaatccc ggtggaggcg acacggtaca ggcattcaag gctggtatca
     5641 tggaaattgc tgacttgttt attttgaaca aagcagacct ggctggtaca gacaagctgt
     5701 tccgcgaagt ggagcagatg ctcgacctgg tcaagcatga ttctccatgg cgccctccag
     5761 tggtaaaaac tatcacgaca caaggtatgg gaattgatga ggcatggcaa gcgtttctag
     5821 accataagtc gtatcttcag gaaagcaagg aatgggagag acgtcgagct tcccatctgc
     5881 gggaagaggt gtctgagatt gtggaacatg ccgtgcacca gctcattgtt aagcgtttgc
     5941 gcagcgaaga atacaaagac atgatggaag gcgtctctcg tcgtgacatt gatccgtatg
     6001 aagtagcgca ccaattatta cagcaattgg taaaagaaaa atagtattct gaaaagagtt
     6061 gcgaattcat tttcttcctg cgtataatag aaggaaggaa gaaaaggcga tgcgcctttt
     6121 ctttttccct gcactctgag caatcgctca gtcggcgtaa ggaaaggagc aaatttaaga
     6181 tggagcaacg taaatcaatc ccctcaatgg taaaagaccc aaagctgatc gagaagcgac
     6241 gcgagcaaat tattgaagcg gcggttgagc tttttatctc caagggattt cacaaaacaa
     6301 ctacccgaga gatttcccgt gcatcaggat ttagcatcgg tacgttatat gaatatatcg
     6361 aatcgaaaga agatgttctc taccttgtat gtgatgcgat ccacgcaggc gtggaaaaag
     6421 gtctgcagga agtgcttgtt aacggtgtaa gtggggccgt aatgttagaa gcagccatcg
     6481 aaggattcct gcgtgtaatg gatcagatgc aggatcgcgt acttctgatt tatcaggaga
     6541 ttaagtcact tcctcgcgaa acgatgagat atgtgctggc gaaggaagaa gagattacag
     6601 gcttttttga agaaatattg catcgcggca gacgggacgg ctcgctgcag attgatgaga
     6661 attcagttaa gctgatggct cacaatattg tggtgcttgg cgagatgtgg acattccgtc
     6721 gctggagttt acgcaagaat tacacgttgg atgaatttac taaaatgcag acatcgttga
     6781 ttttgcgtga attgacggat aagtaaagag ttgccataaa ccggatgaga aggagaaaag
     6841 tcctgcaccg gttcgattgc agacataaga ttgagtgcgc aggggctatt gcgcgacctt
     6901 gagggaggaa aataaagatg gaaacgacaa tttatcgtcc gaagcacaat gttcgttttg
     6961 tcacgacagc cagcttgttc gatgggcatg atgcctcgat taatattatg cgtcgcatct
     7021 tacaggcctc gggagtagag gttattcacc tcggtcataa ccgctccgta gaggagattg
     7081 taaatgccgc tattcaagaa gatgcgcatg gtattgcgat tagctcgtat cagggtggcc
     7141 acgtagagta ttttaaatac attgtcgatt tgctaaaaga gaaaaatgcc tctcatatta
     7201 aagtgttcgg cggtggtggt ggcgttattg tcccttctga gattcgtgag ctgcacgaat
     7261 atggtgttgc gaaaattttc tctccagatg acgggcgtgt gcatggcttg cagggcatga
     7321 ttaactccat gattgaacag accgattatc cgactccaaa agaggttgag aaggaaatta
     7381 gcgagcttaa aagtcgaagc tggaatgcta tcgggcgcat gattacactg gcggaagaga
     7441 atgtagagag gcaagatcgg gaagtagctg ctacgcttga aaaagtgaat gcgatggtgg
     7501 agacggttcc tgtgcttggt atcaccggaa cgggcggagc tggtaaaagc tcgctgacag
     7561 atgaactggt acggcgtttt ctggagcaat atccagagaa gacaatggcc attctatcgg
     7621 tagacccttc caagcagaaa acgggtggag cgcttctagg tgaccgaatt cgtatgaatg
     7681 ccattaactc acctcgcgta tacatgcgtt ccctggcaac gcggaagtct catagcgagc
     7741 ttagcaaggc cattggtgat gcgattaata ttgtgaaagc ggcaggctat gacttcatta
     7801 ttgtcgagac atccggtatt gggcagggcg atgcgcaaat tacgcaagtg tgcgatattg
     7861 ccatgtacgt aatgacgagc gaatttggtg caccgaccca gcttgagaaa attgatatga
     7921 tcgattatgc cgacattatt gctatcaaca aattcgaacg gcgcggttca gaagatgcgc
     7981 tacgcgatgt aaagaagcag tggacgcgca gtcgtgctat ttttgacatg aaggatgagg
     8041 atattccggt atatggtaca atcgccagcc aattcaacga tccgggcaca aatgtacttt
     8101 ttgtgaacct gatgagggtt gttagtgaga aaacaggcac agaatggccg actacactta
     8161 atatctccgg caataccaaa tctaagaagc aatatgtcat tccgtctgag cgtattcatt
     8221 atctggcgga aatcgtcaat accgtacatc aatataagaa atttaccgaa caacaggtgc
     8281 aaatcgcacg caagctattc cagttgaaag gcacgaagga agaattatta gctcacacag
     8341 gggaacttgc atctgattta cagcaagaaa tcgataagct tatttctcac tatgaagaga
     8401 agctgcatcc tgaaagtaga aaaattctgg ataattggaa gtcgttgaag gaagcataca
     8461 tgaaggatga gttcattacg aaagtgcgcg ataaagaaat tgttacgaag ctgtatacaa
     8521 cctccctgtc tggcacacgc attccaaagg tagcgctgcc gaaattcgaa gaatggggcg
     8581 agattcttcg ctgggctatg agcgagaact tgccaggata tttcccttac accgcaggcg
     8641 tatttccatt taagcgtgat ggtgaagatc cgaagcgtca gtttgcgggc gagggtacac
     8701 ctgagcgaac gaaccgtcgt ttccactact tgtctaagga cgatgcagcg aagcgcttaa
     8761 gtacagcgtt cgacagtgtg actttatacg gggaagatcc tgattatcgt ccggatattt
     8821 atgggaaaat tggcacaagt ggcgttagca tttgtacgct ggatgatatg aaaaaactat
     8881 atgatggctt tgacttatgt catccatcaa catctgtatc aatgacgatt aatggacctg
     8941 cgccaattat tctggcaatg tacatgaacg cagccattca gcagcagatc gacaagttca
     9001 agcaagagaa cggacgagaa ccgaatgctg aagaagcgaa gcaaattaag gcctatacgc
     9061 ttaaaagcgt acgcggaacc gtacaggcag atattctgaa agaagatcaa ggacagaata
     9121 catgcatctt ctcgacagaa tttgctctga agatgatggg agatattcag gagtacttca
     9181 tcgagaacca tgttcgcaat tactattcgg taagtatttc cggttatcat attgctgaag
     9241 caggtgcaaa tccgatttcc cagcttgcct ttacgctggc gaatggcttt acgtatgttg
     9301 aatattattt gagccgcggt atgcacattg atgactttgc accaaacttg tcattcttct
     9361 ttagcaatgg tcttgatccg gagtacactg tgcttgggag agttgcccgt cgcatttggg
     9421 caacggttat gaagaacaag tatggcggta atgagcgcag tcagaagctc aagtatcata
     9481 ttcagacgtc aggtcgttcc ctgcatgcgc aggagatgga ttttaacgat atacgcacta
     9541 cattgcaggc gctgatggct atatatgata attgtaactc gttgcatacc aatgcgtatg
     9601 atgaggccat tacgacgcct acagagaatt ctgtgcgccg tgctatggca atccaaatga
     9661 ttattacaaa agagctcggt ttggcgaaaa atgagaattc gttgcaaggt tctttcatta
     9721 tcgaagagtt gactgagtta gtagaagagg ccgtattggc tgaattccag cgtattagtg
     9781 accgtggcgg cgtgctaggt gcgatggaga cacaatacca gcgcagtaag attcaagagg
     9841 agtccctcta ttacgagacg ctgaagcata acggcgagct tcctattatt ggtgtaaata
     9901 cgttcctgaa tccgaattct tccgaggagg actacgagat cgagcttgct cgtgctacgg
     9961 aagaagaaaa ggtacagcaa attaacaacc tgcgcgattt ccaagaacgt cataaggatg
    10021 ttgccgatgc agcgctacga cgcctaaaag aagtagcgcg ttctggagag aatatcttcg
    10081 ctgaactgat ggagacagtg aaagtggcca gtctcggtca aattacaggt gcgctctatg
    10141 aagtcggtgg acaatatcgt cgaaatatgt agggtcgaat gacgagacaa tagtaaactc
    10201 ttttgtcccg aacagaaact ctgttgagga gaaaataaag ggcgagggca tttttccctc
    10261 gccttcttta tgatcgcgtt atttgaaaaa cgaatgaagc acgagttttc cttgcatttt
    10321 aggtctgaaa ttgtataaaa taaggaatgc tgaataatag ggatagtatg tccgggatgc
    10381 aagaatttgc atcttgttat ttgaaacctg taaagaaggg aagtgccgag gttgagcaat
    10441 gctttgaacc tggaagcaga agaagtcaaa gaaatggcga tggtagatat cgcttatcaa
    10501 atgttgaatc aatcaaaaaa accggcctat taccgagatt tgtataaaga aataacagca
    10561 atgaagaaca tgtcggaatc cgaagcgatg gacatgatag cgcaagtgta tacggaaatt
    10621 aatcttgatg gccgctttat atgcctcggt gaaaatctgt ggggattgaa acgctggtac
    10681 cttgtcgaaa cacaagagga gtctgcggaa agcggtttcc ggggcaaatt cgcattggat
    10741 gacgacgatg actttgagga tgacgaagag cttgaagaag aatatgatga gattgacaac
    10801 gctcatgtag atgacgagga tgaagaagag atagacgatg acttcgccga tgacgagaaa
    10861 gaaacggatg aagaattcga tgaagaactg gacgatgaag aattagagaa tgaagatgaa
    10921 gacgaagacc ttgacatgta atacaaggga agataaaata ctttttgggc tttaaaacta
    10981 tatgatgtat cattaacaaa taaaacaaaa gtgcccctcc gaaaatactc ggttggggat
    11041 ctcttttgtt ttttttattt tctagattca tacagctact tgttttgtgg aaaagtattg
    11101 aatgggcaaa gaaagagggg tttatttgta tggcaaagta tatatttgta actggcggtg
    11161 tggtatcttc tctgggcaaa gggattaccg ctgcttctct gggacgtttg ttgaaaaatc
    11221 gtggtctgca ggtaacgatt cagaagtttg atccgtatat caacgtagat ccaggcacaa
    11281 tgagcccata ccagcatgga gaagtattcg ttactcaaga tggcgcggag acggatcttg
    11341 accttggcca ctatgaacgg ttcatcgaca ttaacctgaa ttccaacagt aatgtaacga
    11401 ctggtaaaat ctattcttcc gttattacga aagagcgccg cggtgattac ctgggcggaa
    11461 cggtacaggt cattccacat attactaatg aaatcaaaga gcgtgttttc cgtgcaggac
    11521 gtgagacgaa tgctgatgtt gttattacag aaatcggcgg tacggtagga gatattgaga
    11581 gccttccgtt tctggaagca attcgtcaga ttaagagcga tgtcggtcgt gacaacgtaa
    11641 tgtatatcca ttgtacactt gtgccatact tggcagctgc cggcgagatg aagacgaagc
    11701 cgactcaaca cagtgtgaaa gagctgcgta gcttgggaat tcagccgaat gtaattgtat
    11761 gccgcacaga gcatccgatg tcacaggaca tgaaagataa aatcgcgctc ttctgcgata
    11821 tcgatccgaa agcggttatc gaaatggtgg atgccgaatc gctgtatgaa gttccattga
    11881 ctctgaaaga gcagggagcg gacgaatatg tgtgcaacca tttgaacctc acttgcccgg
    11941 aagcagatat gacagaatgg aaacagatgc tccataaaat taagaacctt tcccacaaga
    12001 cgaccattgc tatcgtcggt aaatacgttg cgctgcatga tgcatatatc agcgtggcag
    12061 aagcattgta ccatgccggt tatgcgaatg atgcggacgt gaatattgag tgggtggatg
    12121 ctgaggaagt gtacgagcat aacgtttctg agctgctgag ccgtgtggat ggtattcttg
    12181 taccaggtgg tttcggagat cgtggtgtag agggtaaaat catcgccgct aagtacgcac
    12241 gtgaaaacaa aattccgttc cttggcattt gtttgggaat gcaggtagcg gttatcgaat
    12301 ttgcccgtca cgtcgtcggc atgaaggatg cgaacagttc ggaaattaat ccgtctaccc
    12361 aatatccggt tattgacctg cttccggatc aaaaagatat tgaaaatctc ggcggtacga
    12421 tgcgccttgg cctgtatcct tgcaaaatgg aaaaaggcac ggcagcccac acatcttacg
    12481 atgaagatct tgtatatgag cgtcatcgcc atcgctatga gttcaacaac cagtaccgtg
    12541 aagcactgaa agatgcaggg ctgaagttca gcggtacatc gccggacgga cgtctggtag
    12601 aaatcgttga gctgccggat cacccttggt ttgtggcaag ccaattccat ccagaatttg
    12661 cctctcgtcc gaaccgcccg cagccacttt tccgcgattt tgttggtgcg gcgttgaagc
    12721 tgaaaaaaca atagtaaata aaaccaaggc gagcgattgc tcgtcttttt ttatggaaaa
    12781 cggacgagcg aaggcgttca tgagtttttc ttgttgtatc atagataaaa agcaggaatt
    12841 ttttaggatt tatcgaactc ttgtattatt gtagagtcag catacatatt tgttgtgcat
    12901 gagaatgaga gggggagcag catgaaggat aagaaagtat tgatagtcga tgaccaatac
    12961 gggattcgag ttctgctgtt tgaggtattt ggaaaggaag ggtaccagac gtttcaagct
    13021 gccaatggaa agcaggcatt agaaattgtc acgggcgagt cgcctgacct tgtcattctt
    13081 gatatgaaaa ttcccggcat ggacgggctt gagattttga aggaaattaa aaaaatcgct
    13141 ccagaaacca aggtgatcat gatgacagcg tatggcgaat tggatatgat taaagaggcg
    13201 acggaactcg gcgctctcac ccatttcact aagccgtttg atattgatga actacgaacg
    13261 gcagtaaata agcaactcgc atgctgatct ttcattgtgg tataataagc aatgtttgag
    13321 ggtatgcaca aaacaaggag gatcttacat atgccattag tttcgatgac agacatgtta
    13381 aataaagccg tggaagggaa atatgccgtc ggtcaattta acttgaacaa tcttgagttt
    13441 acgcaagcca ttctgcaggc ggcagaagaa gagaaatccc ctgtcatcct tggagtaagt
    13501 gaaggtgcag ctcgttatat gggcggtttt aaactggttg tagctattgt acaagcttta
    13561 atggaagaat ataaaattac tgtcccggta gcgattcatc tggatcatgg ctcttccttc
    13621 gagaagtgcg tagaagctat ccatgccggt tttacctccg ttatgatcga tggctctcat
    13681 tatccgctcg aagagaacat tgccctaacc aagcgtgtcg tagacgtggc acatgcgtta
    13741 ggggtgtcag tagaggctga actgggccgt attggtggtc aggaagatga tttgattgta
    13801 caagatgcag aagcggcgta tgcaattccg gcagaatgtg ataaacttgt gcgcgagaca
    13861 ggtgtggatt gcttcgctcc ggcgcttggc tctgttcatg gcccatataa aggcgagccg
    13921 aatctaggct ttgacagaat ggaagaagtt atgaagttga ctggagttcc gcttgttctg
    13981 cacggtggta caggcattcc gacaaaagat atccaacgtg caatttcgct tggtacggcg
    14041 aaaattaatg tgaatacgga gaaccaaatc gcatcagcta aaacagttcg tgaagttctc
    14101 gatgcaaaac cagatttgta tgatccacgt aaatatctgg gacctgcacg tgataccatt
    14161 aaagaaactg ttaagggcaa aatgcgcgaa tttggaagca gcaacaaagc gtaacaccat
    14221 tttgtcagtg tgaagacaga aggagtgtat tatgagattt tttattgata cagcaaatgt
    14281 aaatgagatt cgcgaagtga acgagtgggg cgttttggcc ggagttacga ctaatccttc
    14341 ccttgttgcg aaagaaggac gcgactttgt agagacatta aaagaaatca ttgatatcgt
    14401 tgatgggccg attagtgctg aagttatcag cctggaagcc gaaggcatga ttgaagaggg
    14461 cgagaagctt gcctctcttt ccaagaatat cgtgattaaa gtgccaatga cggtagaagg
    14521 cctaaaagcg gtcaaacatt ttaccaagaa aaaaatcaaa acgaatgtga cgctagtatt
    14581 ctcggcgaca caagcacttt tggctgcgcg tgcaggagcg acttacgtat cgcccttcct
    14641 tggacgcctc gatgatatct cccaagacgg tttgcagtta atttcggaca tctctcaaat
    14701 ttttgatgct catgatattg aaactgagat tatttcggcg agtgtacgcc atccactgca
    14761 cgtagtagaa tgcgcgaaga tgggagcgga catcgcgacc attccgtaca agattttcaa
    14821 gcaaatggca cagcatccgt taacggatgc cggcatcgaa cgctttcttg cggattggga
    14881 tcaagcgcag caaaaataat accggaaaga agcacctttt caaaaagtga tgtgaaaagg
    14941 tgctttttcc cattgagtaa ggagaatcga tctgtggaga aattgtttat cgagggcgga
    15001 cggaagctga cagggcgaat tcaaatcagc ggagcgaaga atagtgcggt ggcgctcatt
    15061 cccgctgcga ttctcgcagg cgcgga
//
