LOCUS       c00145_NZ_CABK..       16241 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CABKTP010000145.1 Clostridium polynesiense isolate
            MGYG-HGUT-01479, whole genome shotgun sequence.
ACCESSION   c00145_NZ_CABK..
VERSION     c00145_NZ_CABK..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:45:19
            Original ID  :: NZ_CABKTP010000145.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 16241
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..16241
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4444:12947]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [AcetylCoA_hydro, CoA_trans,
                     HBT, Lys-AminoMut_A]) and BKACE and LAM_C and Radical_SAM
                     and B12-binding and OAM_dimer)"
                     /neighbourhood="10000"
                     /product="Lysine_degradation"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4445..12947
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [AcetylCoA_hydro, CoA_trans,
                     HBT, Lys-AminoMut_A]) and BKACE and LAM_C and Radical_SAM
                     and B12-binding and OAM_dimer)"
                     /neighbourhood="10000"
                     /product="Lysine_degradation"
                     /protocluster_number="1"
     cand_cluster    1..16241
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [AcetylCoA_hydro, CoA_trans,
                     HBT, Lys-AminoMut_A]) and BKACE and LAM_C and Radical_SAM
                     and B12-binding and OAM_dimer)"
                     /kind="single"
                     /product="Lysine_degradation"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..16241
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Lysine_degradation"
                     /region_number="1"
                     /rules="(minimum(1, [AcetylCoA_hydro, CoA_trans, HBT,
                     Lys-AminoMut_A]) and BKACE and LAM_C and Radical_SAM and
                     B12-binding and OAM_dimer)"
                     /tool="gutsmash"
     CDS             complement(3..755)
                     /locus_tag="ctg145_1"
                     /transl_table=11
                     /translation="MNKNSFVKEYEVRYYEVDYKQRLLPTALLNYFGDMAMAHSEKCGV
                     GLEYLKEKNLAWVLYKYDIEIFKYGILNEKIKITTEAYGFKKFYAYRNFYAENSRGEKI
                     AEGKAVFLLINYERRRPVRIPEDFYAAYGAEGDIKEEIKLYKLQRLTDAAEERTFTVRY
                     TDIDTNTHVNNVKYLDWLIEAVPLNIVKNSFLKNIKIDYLKECFYGDTIKSKAEIFNEE
                     NSTKIIHKIVNQEDKEITLAETYWVKKNE"
     CDS             complement(829..1011)
                     /locus_tag="ctg145_2"
                     /transl_table=11
                     /translation="MEITKDMTIGYIIMMYPDAIDVLMEMGLGCVGCPASQVETLEESA
                     MVHGLDLDMMLQQLN"
     CDS             complement(1119..1613)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acetyltransf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg145_3"
                     /sec_met_domain="Acetyltransf_1 (E-value: 1.9e-15,
                     bitscore: 47.3, seeds: 223, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRKAIKEDIERIMIIVNAAVLKMNQQGNDQWGEDYPKEEDYLKD
                     IEEDNLYVYCEDEEIQGVVCVNEDEAEEYGDIPWRRQGRALIIHRIAVNPESQGRGIAY
                     KLLSFADDLAMDKGINYIKTDTYSLNKIAQNIFTRSGYIFRGIIHFQRRPEDFYRYDKE
                     L"
     CDS             complement(1652..2938)
                     /locus_tag="ctg145_4"
                     /transl_table=11
                     /translation="MTTFIVLGAQWGDEGKGKMTDYLAEEAEVIVRYQGGNNAGHTVEV
                     ENRQYKLHLIPSGILYDHKLNVIGNGVVVDPEALFSEIDYLENLGVKVTPEKLAVSDRA
                     HVIMPYHKTLDKLKEKARGKNEIGTTGKGIGPCYTDKFERSGIRICDLMNKDVFEEKLK
                     ENLSLKNKYITQVLGGEALNFKEVFDVYMEYSERLKPYVKDTSVLIYEKIKDNSSVLFE
                     GAQGMLLDIDYGTYPYVTSSNTTAGGVSSGTGIGPSMISRAIGIAKAYTTRVGKGPFPT
                     ELEDATGDWIRERGHEYGVTTGRSRRCGWLDLVILKTTARVSGLTSFAVTKMDTLAGLE
                     KLKVCIGYKFKDKIIDYFPASLEDLAECQPIYEEFEGWGEEITQAESYEGLPQNAKKYL
                     KRIEEFTGTKVSVISVGPKRNQTIRVGDI"
     CDS             3158..3313
                     /locus_tag="ctg145_5"
                     /transl_table=11
                     /translation="MKKFFKSLLISAGYIAIHFACMVFIFFSSTVFSILSSIKYNYSLS
                     YQDFNN"
     CDS             3347..4003
                     /locus_tag="ctg145_6"
                     /transl_table=11
                     /translation="MALLSLLFYKLIFKLNKKSFFKSLNIRKLSIKLSLLLVLFTIIFS
                     IFDLTLINLIYPMFPGYARVNETITGITQNFSGILAAVIIAAVFEEILFRGLVFKELSK
                     GMNISAAIILQALFFGLAHGNKLQFIYAFYGGIILGLIYYWTNSLAAPILVHFLSNLLG
                     GVVLRRVLYYSSFMIYVYLIAGGVLSILLLIYIYKNKPQKPISFIEESSSVERCQ"
     CDS             4445..4837
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_7"
                     /sec_met_domain="HBT (E-value: 1.5e-07, bitscore: 21.9,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAVIKVRMSLQDAHYGGNLVDGARMLQLFGDVATELLIRNDGDE
                     GLFKAYDSVEFMAPVYAGDFIEATGEIISSGKSSRKMAFEAKKIIIPRPDINDSAAELL
                     EEPVVVCRASGTCVVPVSKQRNKEVL"
     CDS             4839..5657
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: BKACE"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_8"
                     /sec_met_domain="BKACE (E-value: 9.2e-109, bitscore: 353.1,
                     seeds: 244, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LEKLIITAAICGAEVTKEHNPSVPYTVEEIAEEAYKSYKAGASII
                     HLHVREDDGTPTQSKERFSKCINAIKERCPDVIIQPSTGGAVGMTNDERLQPIELLPEM
                     ATLDCGTCNFGGDDIFINTENTIKYFGKRMIELGIKPELEVFDKGMIDMALRLQKKGYI
                     SIPMHFNFVMGVNGGISASPRDLVFMAGSIPEGSTFTVSGIGRNQFTMAAMSIIMGGHV
                     RVGFEDNVYISKGIPASSNGELVEKVVRLAKELNREIASPEEARTILGLK"
     CDS             5721..6755
                     /locus_tag="ctg145_9"
                     /transl_table=11
                     /translation="MKKGCKYGTHRVLEPQGVLTQAAYKIDNSMEIYSNELLIDVQSLN
                     IDSASFTQIEEEAQGNVEKIKEKILSIVNERGKMQNPVTGSGGMLIGTVAAIGDDLKHR
                     DLKVGDKIASLVSLSLTPLKIYEILDVKKDIDRVDVKAQAVLFESGIYAKLPSDMSEPL
                     ALAALDVAGAPAQTAKLVKPGDSVLVLGAAGKSGMLCCYEAMKRVGPTGNVIAMVRKQA
                     QADKLLSWGLCHKAIVSNATAPIDVLNKTLEANKGREVDISISCVNIPNSEMSCILPVR
                     DNGIVYFFSMATSFTKAALGAEGIGKDVNMIIGNGYTKDHAEITLWELRESATLRKIFD
                     EMYL"
     CDS             6771..8045
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: Radical_SAM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: LAM_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_10"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-14, bitscore:
                     43.1, seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="LAM_C (E-value: 1e-44, bitscore: 142.0,
                     seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVNRRFELFKDVPNEEWQDWKWQIRNKVETLEELKKYLPLTKAE
                     EEGVKECLGTLRMAITPYYLSLIDPQDSHDPIRKQAVPTALELKKSSADLLDPLHEDTD
                     SPVKGLTHRYPDRVLLLLTDQCSMYCRHCTRRRFAGHTDSPLPMPQIEKAIEYIKNTPQ
                     VRDVLLSGGDALTLSDDKLEYIIKKLREIPHVEIIRLGSRIPVVLPQRITEDLVNMLKK
                     YHPIWINTHFNHPNEITEEAKAACARLADAGIPLGNQSVLLKGVNDCVHVMKELVHGLV
                     KIRVRPYYIYQCDLSYGLEHFRTPVSKGIEIIEGLRGHTSGYCVPTYVVDAPGGGGKTP
                     VMPNYVISQAPGKVILRNFEGVITTYTEPESYKEECHCEYCTNEKSSSLIGVASLIKGD
                     RLSLEPENLSRKNRITSKEIELNTI"
     CDS             8067..9095
                     /locus_tag="ctg145_11"
                     /transl_table=11
                     /translation="MKPSLRSLIKDYNSISIMGMCKNAGKTEVFNYILKETSKEDIILG
                     ITSIGRDGEEVDAVTLTKKPRIYINKNTILATARQSLSQCDFTKEVLYTTGINTPMGEI
                     VLLKALSCGYADLSGPSTLFELSEVMNKLKELGASKLLIDGALSRKSLATPNITDGCIL
                     AAGAALNSSMEKVIDDTVHTVRLFSLDAIKDSRVKAIAEAHGENNVVVIIDKDYHTKKL
                     SVISSLHSSKEILENLESSSRYIVINGSVTDRLLMDLFSVRELLKDIFIIIKDSTKLMV
                     SKEVFEKFMLTGARIAVLRRINLLAVTINPTSPSGYEFNGEVFKDSLEKSLSVPVFNIF
                     SE"
     CDS             9128..10600
                     /locus_tag="ctg145_12"
                     /transl_table=11
                     /translation="MKFLSHSILEASGFNFVFNEFTPLTPYGISLKKSLPIFMPGEEHK
                     LQQHFLLLEKLIEDMKDKTSLYNELKLCFMKFKDISGTIRLCRNNAVLDEVEIFEIKNL
                     CINIIEFQNLFESLNLKKLMSIEKLKDVKSVLELLDPEDKKLPTFFIYEGYSPKLKDIR
                     SRKKDVEQRIFNSPPEELQELKSLRLSIVNEELQEELLIKEQLSYELHLLISDITDNLA
                     LIGELDILIARGNLALRYKGVKPELTKDLSIDISSAVNPFVEKLLKVKGKSFKELSIKL
                     EGGSTVITGANMGGKTVALKTLVLNIALVHYGFFPFCKSIRLCLFNFIYFSSEDKQSLS
                     KGLSSFGAEIMEINEVLALCNRQRGFIALDEFARGTNPKEGSILLRSLTSYLNSLNSIA
                     VIATHYDGIAKEAKAHYQCVGLKKTDFNSLNEKIKNSRENSVDILQEYVDFTLEKVSAS
                     SDVPRDALNICRLLNFPEEILNKAVKLYEKGD"
     CDS             10604..12157
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: Lys-AminoMut_A"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_13"
                     /sec_met_domain="Lys-AminoMut_A (E-value: 1.8e-274,
                     bitscore: 900.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESKLNLDFKKVEKARNYAKNIALESQEFIEKHTTTTVERAVCRL
                     LGIDGVDAFGVPLANLVVENISKGGGLPLGAAYFIGNAMAETSLTPQEIAEKVSKGELD
                     LTKIKQEDIFNIKLAIKPIVDNTIERIKNNKAEREAYLEKYGDKSGPLIYVIVATGNIY
                     EDIIQAKAAAKQGADVIAVIRTTGQSLLDYVPYGATVEGFGGTFATQENFRLMRKALDE
                     VGEECGRYIRLCNYCSGLCMPEIAALGAVERLDVMLNDALYGILFRDINMKRTMIDQFF
                     SRVINGFAGVIINTGEDNYLTTADAVEEAHTVLASQLINEQFALLAGLPEEQMGLGHAF
                     EINPDLKNGFLYELAQAQMAREIFPKAPLKYMPPTKFMTGNIFKGHVQDALFNMITVMT
                     SQRIHLLGMLTEAIHTPFMSDRALAIENANYIGNTMADLGDEITFKKDGIMDKRAEEVV
                     DKACALLEEIQEEGLFKTLEKGIFAGIKRPINGGKGLDGVIEKHESYFNPFIQPMMRRD
                     "
     CDS             12159..12947
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: OAM_dimer"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Lysine_degradation: B12-binding"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_14"
                     /sec_met_domain="B12-binding (E-value: 2e-23, bitscore:
                     73.0, seeds: 91, tool: rule-based-clusters)"
                     /sec_met_domain="OAM_dimer (E-value: 3.8e-36, bitscore:
                     113.7, seeds: 55, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSGLYSTNKKEFDKTLDYSKLKPYGDTMNDGKVQLSFTLPVSDG
                     DKACEAAKLLAKKMGLEEPNVAYHSPLDNDFTYFVVYGSINHTVDYNSIKVQSVDVDKM
                     SMEDINEYIKDNIKRKVVVLGASTGSDAHTVGIDAIMNMKGYAGHYGLERYEMIEAYNL
                     GSQVLNEEFVKKAIELKADVLLVSQTVTQKNIHIENLTELVELLEAEGIRHKVVLICGG
                     PRITHELAKELGYDAGFGPGKYADDVASYAVTEIVNRKLI"
     CDS             complement(13180..14547)
                     /locus_tag="ctg145_15"
                     /transl_table=11
                     /translation="MNSDFLIPVIIAAVIIIVIIGLLSMWKKVPQDKAVVVTGLKKRVI
                     SGGGGVVIPILERVDRISLENMKIEIRIGNALTEQGVGITADGVAVIKVKSDLESVLAA
                     VEQFNTGNEDKTIDIIKDTSKDVLEGKLREIVSKLTVEEIYKDREKFASRAQEVAAIDL
                     GQMGLEIKAFTIRDISDNNGYLQALGKGRIAEVKRDAEIAESEAQKETKIKTSEAFKEG
                     EAAKLIAETFVAESLKNKELKVQAYRKEQETAKAMADLAYEIEANKVKKEVTETSMLVE
                     IIKKEKEIELAEQEALRKEKELDGTVKKQSEAIKFESIQEAQAKAESIKLEGQARAEAM
                     KLEGMAKVEIIRETGKAEAEAMVKKAEAYKLYNDAAMAQMIIEKLPDIAKAVAEPLSKT
                     EKIVIMDSGSTASGGGAAKVTGYVTDIMSKLPETIKATTGIDLIEMLKKSQSGSEG"
     CDS             complement(14594..15106)
                     /locus_tag="ctg145_16"
                     /transl_table=11
                     /translation="MRSVYYLFFCIGVVYTIITFVLGGIFDSFDISGEGSFEGVLSSLK
                     PSVIISFCTIFGGIGLMTLNKLPWYLGLSISLLSAFLTAYIINKFIIVPLYKSQNTSAG
                     EKAEFIGREALVISPILEKSYGTISYSYKGNTYTSPAKSIEGERIDQGLTVIIIKIEDN
                     LCYVMQK"
     CDS             15244..15879
                     /locus_tag="ctg145_17"
                     /transl_table=11
                     /translation="MDINRKIAPKRYDFKSVENISSDQLQQHYKLYIGYINKFNEINSE
                     SRNASDYPDSNPTYSKMRCLKLGESYALDGVKLHELYFENLTNHSCCASKELMALINRD
                     FINYDNFIEYFKNAALAMRGWVILTIDPLTDSLLVIGCDAHDVGAIWNSYPLLVLDVYE
                     HAYMIDFGIDRKAYINAFIKNINWNVVNKRLQQYLNMKNSQMQMRKYQ"
ORIGIN
        1 ttttcatttt tctttaccca gtaggtttca gccaaagtta tttccttatc ctcttgattt
       61 actattttat gtatgatttt agtgctgttt tcttcattaa atatttctgc tttagatttt
      121 atggtgtctc cataaaagca ttccttaaga taatctattt ttatattttt taaaaagcta
      181 tttttcacta tattaagagg cactgcttct ataagccagt ccagatattt aacgttattt
      241 acatgggtat tggtatcaat atctgtatat cttaccgtaa aggtcctttc ctccgctgcg
      301 tcagtaagcc tttgaagctt gtaaagcttg atttcctctt taatatcccc ttctgctcca
      361 taggcggcat aaaagtcctc aggaattcta accggtcttc ttctttcata gtttataaga
      421 agaaataccg ctttaccttc tgctattttt tctcctctgc tgttttctgc ataaaaattt
      481 ctataggcat agaacttctt aaaaccataa gcttctgtgg taatctttat tttttcattt
      541 aaaattccat atttaaatat ttctatatca tatttataca aaacccatgc cagattcttt
      601 tcctttaaat actccagccc tacgccgcac ttttcagagt gagccattgc catatctccg
      661 aagtaattta aaagagcggt gggaaggagt ctctgcttgt aatccacctc ataatatctc
      721 acttcatatt ccttaacaaa gctgttttta ttcatgagtt ttcctcctta acaaagaaac
      781 gtatacctct attatacaga ggtatacgtt aaaagtcttt ataggctatc aatttaattg
      841 ctgaagcatc atatctaaat caagtccatg gaccatagct gattcctcta gagtttctac
      901 ctgagaggcg gggcagccta cgcatccgag gcccatttcc atcagcacat ctatagcatc
      961 agggtacatc ataattatat agcctatggt catatccttc gttatttcca tgtaatcatc
     1021 cccaaaaata tttttcttac tatttgtaag ttatacctac attataatac aaatgaggat
     1081 aattatcaat tacaattatc ttctttaatt aagcactgct ataattcctt atcatagcgg
     1141 taaaaatctt caggcctcct ctgaaaatgg attatacctc tgaatatata tccggatctt
     1201 gtaaatatat tttgagcaat tttatttaat gaataggtat ccgtttttat gtagtttatt
     1261 cccttatcca tcgccagatc atcagcaaaa cttaaaagct tataagctat tcctcttccc
     1321 tgactttcag gattaacagc aatcctatga ataattagag cccttccctg tcttctccag
     1381 ggtatatccc catattcctc tgcttcatcc tcatttacgc atactactcc ttggatttct
     1441 tcatcctcac agtatacata taaattatct tcttcaatat cctttaaata atcctcttct
     1501 ttgggataat cctctcccca ttggtcattc ccctgctgat tcatttttaa aaccgcagca
     1561 ttaactataa tcataatcct ctcaatatcc tctttgatag cttttcttat catataacct
     1621 cctgaaaaac taagtttata atctctgctt cttagatatc ccccactctt atggtttgat
     1681 tccttttagg tcctacagaa ataacagaaa cttttgtccc tgtaaattcc tctatcctct
     1741 ttaaatactt tttagcattt tgaggaagcc cttcatagct ttctgcctgt gttatctctt
     1801 ctccccatcc ttcaaattct tcataaatag gctggcactc cgctaaatcc tccaggcttg
     1861 caggaaagta gtcaattatt ttatctttaa atttataacc tatacatacc ttgagctttt
     1921 ctaggcctgc aagggtatcc atttttgtca ctgcaaagct ggtcaagcct gaaactctgg
     1981 cagtggtttt aagaattaca agatcaagcc atccgcacct tctggatctg cctgtagtta
     2041 ctccgtattc atggcctctt tctcttatcc agtctcctgt ggcatcttca agctctgtgg
     2101 gaaagggtcc ttttcccacc cttgtagtat atgctttagc tatacctatt gcccttgaaa
     2161 tcatagaagg accaattcct gttccgctgc taacacctcc tgcagtggta ttggaggaag
     2221 ttacataggg ataggtgccg tagtctatat ccaaaagcat tccctgggct ccctcaaaca
     2281 aaacgctgct gttatctttt attttctcgt atataagtac agaagtgtcc ttaacataag
     2341 gctttaatct ttcactgtat tccatataca catcaaaaac ttccttaaaa tttaaagcct
     2401 cgccccccag tacctgagtt atatatttat tttttaaaga cagattttct ttaagctttt
     2461 cttcaaacac atccttattc ataagatcgc agattcttat gccggatctt tcgaatttat
     2521 cagtatagca tggtcctata ccctttccgg tagtgcctat ttcattttta cctctagcct
     2581 tttccttcag tttatccaat gttttatgat aaggcataat aacatgagcc ctgtcactta
     2641 cagcaagctt ttcaggagtt accttaaccc ctagattttc aagataatct atttcgctga
     2701 ataaagcttc aggatccacc acaacaccgt ttcctataac attaagctta tggtcatata
     2761 atatccctga aggtattaaa tgaagcttat actgtctgtt ttcaacctct acggtgtgtc
     2821 ctgcgttgtt tcccccctga taccttacta tcacctctgc ttcctcagct aaataatctg
     2881 tcatcttgcc ttttccttcg tctccccatt gcgctcctaa aactataaat gttgtcatag
     2941 ctttcctcct tataaacagc attaaatttt aatgcctgaa tagtttagca aaactacaat
     3001 ccaccctatt accctatagt tataatatct tctgtttttt attattaatt ccctcaaggt
     3061 cagcttaaat tataaataaa tactatcaaa gatgaattga acagtcaaaa gttgtataat
     3121 tatggtatta aatatattta tttacacgga gtgttttatg aaaaaatttt ttaagtcttt
     3181 actgatttct gccggataca tagcaatcca ttttgcatgt atggtattta tatttttttc
     3241 ctctactgtg tttagtatac tttcatctat taaatacaat tacagtctgt cctaccaaga
     3301 ctttaataat tagattatgc agatatctgg aattctatat ttacttatgg ctttactatc
     3361 cctgctcttt tataaattaa tatttaaact gaataaaaaa agctttttta aatcattaaa
     3421 tataagaaag ctttccatta aactctcact gcttctggtt ttattcacca taattttttc
     3481 aatttttgat cttactctga taaacctcat ttatccaatg tttcccggct atgcccgggt
     3541 aaatgaaact ataacaggca tcactcaaaa cttttccgga attttagctg cggtgattat
     3601 agccgccgtc tttgaagaaa tcctctttag aggtcttgtt tttaaagagc tatctaaagg
     3661 gatgaatatt tcagcagcta taatacttca ggcactgttt ttcggtctag ctcacggaaa
     3721 taagcttcaa ttcatatatg ccttttatgg aggaattatt cttggtttaa tatattactg
     3781 gacaaactcc ctggcagcac ctatattagt ccacttcctt tctaatttat taggaggagt
     3841 tgtattgcgc agagtacttt attattcctc ttttatgatt tatgtttatc ttatagccgg
     3901 aggagtattg agtattttac tccttatata tatttataaa aacaagccac agaagcctat
     3961 aagttttata gaagaaagtt cttctgtcga aagatgtcaa taaataattg ccataaatta
     4021 gttaaaaaat cgtttacaat aaattaagta ttaaatataa tataattaga cgaatatggt
     4081 taacccataa atagaggcgc aggtaaatag tgtagcttaa agatgttaag gagaagaacc
     4141 ttcaaagctt taagcaaagg gtttatctgc cgaaagtcag gatgagttct tttgtcctgc
     4201 cttggaaata aagtacacag ctttattttt gccgtaggtt acggagcgct atttatgtaa
     4261 tgaaattact tcattgcatg gatagcaaat cttaaataag tctatccagg cactgatgat
     4321 ttcagtgctt tttcttttct ttcagctaat cttaaaaaca gttaaaagcc acccagggaa
     4381 tgtttcttaa tataaatata tcactatttg ttaaattttt aacaaatata aaaggaggtt
     4441 tattatgaaa gcggttatta aagtaaggat gagccttcag gatgctcatt atggaggcaa
     4501 ccttgtagat ggagccagaa tgcttcagct ttttggagat gttgccacag aactactcat
     4561 aaggaatgat ggagatgaag gactttttaa ggcttatgac agcgtagagt ttatggctcc
     4621 ggtttatgcc ggagatttca ttgaagccac aggagaaatt atatcttcag gaaaatcctc
     4681 aagaaaaatg gcatttgagg caaaaaaaat aatcattcca agacctgata taaatgactc
     4741 cgctgcagaa cttttagaag aaccggtagt tgtgtgcaga gccagcggta cctgcgtagt
     4801 acctgtgtcc aagcaacgaa ataaggaggt tttataattt ggaaaagctt ataattacgg
     4861 cagctatctg cggtgctgaa gttacaaaag aacataatcc aagtgttccc tacaccgtgg
     4921 aggaaattgc agaagaagca tataagtcct ataaggcagg agcaagcata atccaccttc
     4981 atgtgaggga ggatgacgga actcctaccc aaagtaaaga aagattcagt aagtgcatta
     5041 acgccattaa ggaaagatgt cctgatgtca tcattcagcc atctaccgga ggagctgtag
     5101 gaatgaccaa tgatgaaagg cttcagccta tagagctttt acctgaaatg gcaactttag
     5161 actgtggaac ctgtaacttt ggaggggatg atatcttcat aaacacagag aacactataa
     5221 agtactttgg aaaaaggatg atagagctgg gcataaaacc ggagctggaa gtctttgata
     5281 agggtatgat cgatatggct ttaaggcttc agaagaaagg ttatataagc attcccatgc
     5341 attttaactt cgttatggga gtaaacggcg gaatttccgc ttcacccaga gatttagtgt
     5401 ttatggcagg aagcatacct gaaggctcca cctttaccgt ttccggtatc ggaagaaatc
     5461 agtttaccat ggcggctatg tcaataataa tgggtggaca tgtaagggta ggctttgaag
     5521 ataatgttta tatttcaaaa ggaattcctg cttcctccaa cggggagctg gtggaaaagg
     5581 tagtgaggct tgctaaggag cttaataggg aaatagcatc tcctgaggaa gcaagaacaa
     5641 ttctaggctt aaaatagatt ttttcattat tataagtaaa aatctatata ataaaatttt
     5701 catattaggg ggaattattt atgaaaaagg gttgcaaata tggtacacat agggttttag
     5761 agccacaggg tgttttaacc caggccgcct ataaaatcga taacagcatg gagatatact
     5821 caaatgaact cttaatagat gttcaatctc taaacataga ctctgcaagc tttactcaaa
     5881 ttgaggagga agctcagggc aatgttgaaa agataaagga aaaaatactt tccatagtta
     5941 atgaaagagg aaagatgcag aatcccgtta caggttccgg cggaatgctt ataggtaccg
     6001 ttgccgctat aggggatgac cttaaacaca gggatttaaa ggttggagac aaaatagctt
     6061 ctttagtatc cttaagcctg actccattaa aaatatatga aatcctggat gtaaaaaaag
     6121 acatagacag agtggatgta aaagctcagg ctgttttatt tgaaagcgga atttatgcaa
     6181 agcttccttc agatatgtca gagcccttag cactggcagc tttggatgta gcaggagctc
     6241 ctgctcaaac cgcaaagctt gttaaacccg gcgacagtgt acttgtttta ggagctgcag
     6301 gtaaatccgg aatgctgtgc tgctatgaag ccatgaagag agtaggtcct acaggaaacg
     6361 ttatagctat ggttagaaag caggcacagg ctgataagct tctatcctgg ggattatgcc
     6421 ataaagccat agtatccaat gctactgctc ctattgatgt gctaaacaaa accttagaag
     6481 ccaataaagg cagagaggtg gatatatcca taagctgtgt aaacatacct aattcagaaa
     6541 tgtcctgtat tctccctgta agggataacg gtattgtata tttcttctcc atggcaacct
     6601 cctttactaa ggcagctttg ggcgctgaag gaataggcaa agatgtgaac atgataatag
     6661 gaaacggata tactaaggat catgctgaga taaccctttg ggagctgaga gaaagcgcca
     6721 ccttaaggaa gatattcgat gagatgtatc tgtaaaacgg gaggaagatt atgaaagtta
     6781 ataggcgctt tgaattattt aaagatgtac ccaatgaaga gtggcaggac tggaagtggc
     6841 agataagaaa caaggtggaa actttagagg aattaaaaaa atatcttccc ctcactaaag
     6901 ctgaggagga aggtgtaaag gaatgtctcg gcaccttaag aatggcaata acaccttatt
     6961 atttatcctt aatagaccct caggattctc atgatccaat aagaaaacag gcggttccta
     7021 cagctttaga acttaaaaaa tcctcggcgg atttattaga tccccttcat gaggatactg
     7081 attctccggt taaaggctta acccacagat atcctgacag ggtgctgctt ctgcttacag
     7141 atcaatgctc aatgtactgc agacactgca caagaagaag gtttgccggt catacggact
     7201 cacctcttcc catgcctcag atagaaaaag ccatagaata tataaaaaat acccctcagg
     7261 ttagggatgt gcttttatcc ggcggtgatg ctctcacctt atctgatgat aagcttgaat
     7321 atatcattaa aaagcttagg gaaattcctc atgtggagat aataagactg ggtagcagaa
     7381 ttcctgtggt tcttcctcaa aggattacag aggatttagt aaatatgctg aaaaaatacc
     7441 atcccatttg gataaatact cattttaatc atcctaatga aataaccgaa gaagctaaag
     7501 ctgcctgtgc aagacttgcg gatgcaggaa tacctcttgg aaaccaatca gttttgttaa
     7561 aaggagtaaa tgactgcgtt catgttatga aggaactagt acatggatta gttaagataa
     7621 gggtaaggcc ttactatatt tatcaatgcg atttatccta tggccttgag catttcagga
     7681 ctcctgtatc taaggggata gaaattattg aaggtcttag aggacatacc tccggatact
     7741 gtgttccaac ctatgtggtg gatgctcccg gaggaggagg taaaactcct gttatgccta
     7801 actatgtaat atcccaggct cctggaaagg taatattaag aaacttcgaa ggggttataa
     7861 ccacctatac tgagccggaa agctataagg aagaatgtca ctgcgaatac tgtaccaatg
     7921 aaaagtcttc ttcacttata ggtgttgctt ctcttattaa gggagacaga ttatctttag
     7981 agcctgaaaa tctttcccgt aaaaatagaa taaccagcaa ggaaatagag cttaatacta
     8041 tataaatata caaggagggc tgctgcatga aaccttcact gagaagttta ataaaggatt
     8101 ataattcaat ttctattatg ggtatgtgta aaaatgccgg taaaacagaa gtctttaatt
     8161 atatattaaa agaaacctct aaagaggata taatccttgg aattacttct ataggaagag
     8221 atggtgaaga ggtagatgca gtaaccctca ctaaaaaacc cagaatttat attaataaaa
     8281 acacaatatt agctacagca agacagagcc tttcacagtg cgactttacc aaggaagtgc
     8341 tgtacactac aggaataaat acacctatgg gagaaatagt ccttcttaag gctttaagtt
     8401 gcggatatgc tgacctctca gggccctcca ccttatttga gctttcagaa gttatgaata
     8461 aactaaagga gctgggcgct tccaaactgc ttatagacgg agccttaagc agaaaatccc
     8521 ttgccactcc caatataacc gacggatgta tcttagcagc aggagctgct ttaaacagca
     8581 gcatggaaaa ggtaatagat gatacagtac ataccgttag attattttcc ctagatgcca
     8641 taaaagacag cagagtaaaa gctatagcag aagctcatgg agaaaataat gtggtggtaa
     8701 taattgataa ggattaccat actaaaaagc taagtgtaat cagttctctc cattcttcta
     8761 aggaaatcct tgaaaatcta gagtcctcca gccgctatat cgtgattaac ggctcggtta
     8821 cagacaggct tttaatggat ttattctctg taagggaact gttaaaagat atttttataa
     8881 taataaaaga cagcacaaag cttatggttt ccaaagaggt ctttgaaaag tttatgctta
     8941 ccggagcaag gatagcggtt ttaagaagaa tcaatcttct ggctgtaact attaacccca
     9001 cctccccctc cggttacgaa ttcaatggag aagtatttaa agactcatta gaaaaaagct
     9061 tatcagtacc tgttttcaat atatttagtg aataggcaca aaatttaagc tgtaaggagg
     9121 tatatatatg aagttcttat cacacagtat tcttgaagct tcaggcttta attttgtatt
     9181 taatgaattt actcctttaa ccccttatgg tataagctta aaaaagtctc tccccatatt
     9241 tatgcccggg gaggagcata agctgcagca gcatttttta cttcttgaaa aacttataga
     9301 ggatatgaag gataaaacca gtctatataa tgagctaaaa ctctgcttta tgaaatttaa
     9361 agatatatca ggtactataa ggctctgcag aaataacgcc gtgctggatg aagtagagat
     9421 ttttgaaatt aaaaacctat gtataaatat aatagaattt caaaatctct ttgaaagtct
     9481 taatttaaaa aaactgatga gtatagaaaa gcttaaggat gttaaatcag tacttgaact
     9541 tttagatccg gaggataaaa agcttcctac cttttttata tatgagggct acagccctaa
     9601 gcttaaggat ataagaagca gaaaaaaaga tgtagaacag aggatattca attctcctcc
     9661 tgaggagctt caagagctta agtcacttag actaagcata gtaaatgaag agctccagga
     9721 agaacttctc ataaaggagc agctttccta tgagcttcat cttctaataa gtgatattac
     9781 agataatcta gcactcatag gggaactgga tatacttatt gcaagaggaa atttagcttt
     9841 aagatacaag ggagtaaagc cggaactaac caaggatctt tccatagata tatcctctgc
     9901 agtaaatcct tttgtagaaa agcttttaaa agttaaagga aaaagcttca aggaactttc
     9961 cataaagctg gagggaggct caacggtaat taccggagca aatatgggag gtaagactgt
    10021 agcattaaag actctggtgc ttaatatagc actggtgcat tatggatttt ttcctttctg
    10081 taaaagcata aggctctgtc tgtttaactt catttatttt agttccgaag ataaacagag
    10141 cttatcaaaa ggcttaagca gcttcggtgc tgaaataatg gagattaatg aggtgcttgc
    10201 cttatgtaat agacagagag gatttatagc tttagatgaa tttgccagag gtactaatcc
    10261 taaggagggc tccatactcc ttcgctctct tacctcttat ttaaacagct taaacagtat
    10321 agctgtcatt gcaactcatt atgacggcat agcaaaggaa gcaaaagctc attatcagtg
    10381 tgtggggtta aaaaagactg attttaactc tttaaatgaa aagattaaaa attcaaggga
    10441 aaattcagtg gatatacttc aggaatatgt agattttacc ttagaaaagg tatctgcctc
    10501 ctccgatgtg ccaagagatg ctctgaatat atgcaggctt ttgaattttc ctgaggagat
    10561 attaaacaaa gcggtaaaac tttacgaaaa gggggattaa aatatggaaa gtaaattgaa
    10621 ccttgatttt aaaaaggtag aaaaagctag aaattatgca aaaaatatag ctctggaatc
    10681 tcaggagttt atagaaaagc atactaccac cacagtggaa agagcggtat gcagactttt
    10741 ggggatagac ggagtagatg cttttggggt tcctttagcc aatctggtag tggaaaacat
    10801 atctaaagga ggaggtttac ctttaggtgc tgcttatttt attggaaatg ctatggcaga
    10861 aacctcactg acacctcagg aaatcgcaga aaaggtatct aaaggagagc ttgacttaac
    10921 caagataaag caggaggata tctttaatat aaagcttgct attaagccta tagtagacaa
    10981 cactatagag aggattaaaa ataacaaggc tgaaagagaa gcttatcttg aaaaatatgg
    11041 agataaatcc ggtcctctta tctatgtaat agttgcaaca ggaaatatat atgaggatat
    11101 aattcaagct aaggctgcag caaagcaggg tgcagatgta attgcagtaa taaggactac
    11161 cggtcagagc cttttggatt atgttcccta tggagcaacg gtagaaggct tcggaggaac
    11221 ctttgccact caggaaaact tccgcctaat gagaaaagcc ttagatgagg ttggagagga
    11281 atgcggaagg tatataaggc tgtgcaatta ctgttcaggg ttatgcatgc cggaaatagc
    11341 agccttagga gcggtggaaa gattagatgt tatgcttaat gatgctcttt acggtatact
    11401 cttcagggat ataaatatga aaagaaccat gatagatcag ttcttttcaa gggttataaa
    11461 cggctttgca ggagtaataa taaataccgg tgaggacaat tatttaacca ctgctgatgc
    11521 tgtggaggaa gcccatacgg tactggcttc tcaacttata aatgaacaat ttgccctatt
    11581 agcaggactt ccggaagagc aaatggggtt aggccatgcc tttgagatta atcctgattt
    11641 aaaaaacgga ttcctatatg aattagccca ggcgcagatg gcaagggaga tattccccaa
    11701 ggctcctctt aaatatatgc ctcctacaaa gtttatgaca ggaaacatat ttaaaggaca
    11761 tgtacaggat gccctgttta atatgatcac cgtaatgact tctcaaagaa ttcacctttt
    11821 aggaatgctt acagaagcaa tccatacccc ttttatgtcc gacagagcct tagctataga
    11881 aaatgctaat tatataggaa atactatggc agacttagga gatgaaataa cctttaaaaa
    11941 ggacggaatc atggataaga gagctgaaga ggtggttgat aaggcctgtg ctcttcttga
    12001 ggaaatccag gaggaaggct tattcaaaac ccttgaaaaa ggcatatttg caggaataaa
    12061 aagacctata aacggcggta aaggcttaga cggcgttatt gaaaagcatg aaagctactt
    12121 taatcccttt atccagccta tgatgaggag ggattaatat gagcagcgga ttatattcca
    12181 ctaataagaa ggaattcgat aagactttag attattcaaa gcttaaaccc tacggagaca
    12241 ccatgaatga tggcaaggtt cagctgtcct ttacccttcc tgtatcagat ggagacaagg
    12301 cttgtgaagc agcaaagctt ttagctaaaa aaatgggatt ggaagagcct aatgttgctt
    12361 atcatagtcc tttagataat gactttacct attttgtggt ttacggaagc ataaatcata
    12421 ctgtagatta taattctata aaggtacagt ctgtagatgt tgataaaatg tctatggaag
    12481 acataaatga atatatcaaa gacaatataa agagaaaagt agtggtttta ggagcttcca
    12541 ccggcagtga tgcccatact gtaggcatag atgccattat gaacatgaag ggctatgcag
    12601 gtcattacgg actggaaaga tatgagatga tagaagccta taacctcggc agtcaggtat
    12661 taaacgagga attcgttaaa aaagctatag aacttaaggc tgatgtgctt ttagtttctc
    12721 aaacggtaac acagaagaat atccatattg aaaacctcac ggagcttgta gagcttttag
    12781 aggcagaagg tataagacat aaggttgtgt taatctgtgg cggtccgaga atcacccatg
    12841 agcttgcaaa ggagctcggt tacgatgccg gcttcggtcc cggcaagtat gcagatgatg
    12901 tagcatccta tgctgtcact gaaatagtaa acagaaaact aatttaatcc ttaaaataaa
    12961 tcatgaaatt aaaagcaatt aaattgtaca aaattttatg tacaagtgct ttaatagtaa
    13021 ttataggtat ttatgccgga atttattatt acaacaataa tataaaatta aatgtgcagc
    13081 atataactat ataaatcaaa agtctaattt acaggcacaa taaagaagta aagagaggct
    13141 ttataaagcc tctctttact tctttattac ttcttaaatt taaccttcac tgcctgattg
    13201 gctcttttta agcatttcta tcaaatctat tcctgtagtt gcctttatgg tttcggggag
    13261 cttgctcatt atatctgtaa cataccctgt cacctttgct gctcccccgc ctgaagcggt
    13321 acttccacta tccataataa ctatcttttc tgtcttggaa agaggctccg ccactgcttt
    13381 agctatatca ggaagctttt ctataatcat ttgagccata gctgcatcat tatacagctt
    13441 ataggcttct gccttcttaa ccatagcctc tgcttctgcc tttcccgttt ctcttataat
    13501 ttcaaccttt gccatacctt caagcttcat agcttcagct cttgcctgtc cttccagctt
    13561 tatgctttca gccttagcct gagcctcctg tatagattca aacttaatag cttcagactg
    13621 cttctttaca gtaccatcaa gctctttttc ctttcttaaa gcctcctgct ctgcaagctc
    13681 tatttccttt tccttcttaa ttatttctac cagcatagag gtttcggtaa cctccttctt
    13741 aaccttgtta gcctctattt cgtaagctag gtccgccata gcctttgcag tttcctgctc
    13801 ctttctataa gcctgtacct ttaactcctt atttttcaag gattctgcca cgaaggtttc
    13861 tgctataagc ttggcagcct ctccttcctt aaaggcttct gatgtcttta tctttgtttc
    13921 cttttgtgct tctgattcag caatttcagc atctctctta acctcagcta tccttccctt
    13981 tcctaaagcc tgaagatacc cattattatc tgatatatcc cttatggtaa atgcctttat
    14041 ttccaatccc atctgaccca aatctatagc tgcaacttcc tgagcccttg atgcaaattt
    14101 ttctctgtct ttataaattt cctctacggt aagcttagaa actatctctc ttaattttcc
    14161 ttctaataca tcctttgaag tatctttaat tatatctata gttttatctt catttccggt
    14221 gttaaactgc tctactgctg ctaaaactga ctctaaatcg gactttacct ttattacagc
    14281 cacaccatct gctgtaattc ccactccttg ttcagttaaa gcatttccta ttcttatttc
    14341 aattttcata ttttctaagg atattctgtc taccctttcc agtataggaa ttactactcc
    14401 tccgcctccg gagataactc tcttttttaa tcctgtaact accactgctt tgtcctgcgg
    14461 aacctttttc cacatagata atagacctat aattacaatt attattactg cagctataat
    14521 tacaggaatc aaaaaatctg aattcataaa ccttccccct ttatattgta aaatttatat
    14581 aatccataaa ggattatttc tgcattacat aacacaagtt atcctcaatt tttatgatta
    14641 taactgtgag tccctgatct atcctctctc cttctatact ttttgcagga gatgtatagg
    14701 tatttccctt atagctgtag cttatggttc catagctctt ttccagaata gggcttatca
    14761 ctagagcttc tcttccaata aattcagcct tctcacctgc acttgtattt tgtgacttat
    14821 aaagagggac tattataaat ttatttatta tataagctgt taaaaaagca gaaagaagcg
    14881 agatacttaa ccctaaatac cagggcagct tattcaatgt catcaatcct atacctccga
    14941 atatagtgca gaaggaaatt ataactgatg gctttaatga ggaaagaaca ccttcaaaac
    15001 tgccttctcc gctaatatcg aagctatcaa atattcctcc cagcacaaag gttataatcg
    15061 tataaactac ccctatgcaa aaaaataagt aatatactga cctcattatt acccacccct
    15121 tacaatatat atttattata ccataatatg tatttaatag gatattatgt ttaatatatt
    15181 ttattaacac tatgggtatc ttcaaaatat aatgattatg taataatata tggaaaggtg
    15241 cttatggata taaatagaaa gattgctcct aaaaggtatg actttaagtc cgtagaaaat
    15301 atcagctccg atcagctgca gcagcattat aagctttata taggctacat caacaaattc
    15361 aatgaaataa actcagaaag cagaaatgcc tctgattatc ccgacagcaa tccaacttac
    15421 agtaaaatga gatgcttaaa gcttggagaa agctatgctt tagacggtgt aaagctccac
    15481 gagctttatt ttgaaaatct caccaatcat tcctgctgtg caagcaaaga actcatggct
    15541 ttaataaaca gagactttat aaactatgat aactttattg aatattttaa aaatgccgct
    15601 ttggctatga gaggctgggt gatacttacc atagatcctc ttacagactc tcttttagtt
    15661 ataggctgtg atgcccatga tgtgggagct atatggaact cctaccccct tttagtgcta
    15721 gatgtatatg aacatgccta tatgattgat tttggtatag acagaaaagc ttatataaat
    15781 gcttttataa aaaatataaa ctggaatgta gtaaacaaaa ggcttcagca gtacttaaac
    15841 atgaaaaact cacaaatgca gatgagaaaa tatcagtgat ataattataa taaaagtgag
    15901 aagaatatga atctcctcac ttttattact tcgtcttcca gcttacattt gtttcaaatt
    15961 acataatttt tacaagtctc tataataatt ccagatacaa aatactccaa aaaagaccat
    16021 ccggaatatc ataggttata ttcacaccat acaaaacaga gacttaaaaa taaatctctt
    16081 tagaatattg ttcattgcct taatacttat catcatgggt aatttcctta tgtgcattat
    16141 aagcgccaat aaaagaaaga atacttgaaa ttccatgtaa aattgggagg acaattatgg
    16201 taattaacaa tataaaaaag cagtacactt ttattaaaat g
//
