LOCUS       c00092_NZ_CABK..       14695 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CABKTP010000092.1 Clostridium polynesiense isolate
            MGYG-HGUT-01479, whole genome shotgun sequence.
ACCESSION   c00092_NZ_CABK..
VERSION     c00092_NZ_CABK..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:45:19
            Original ID  :: NZ_CABKTP010000092.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14695
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14695
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[14:4695]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      15..4695
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..14695
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14695
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             complement(15..863)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg92_1"
                     /sec_met_domain="HCDH (E-value: 7.9e-39, bitscore: 122.3,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 9.8e-73, bitscore: 233.8,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKIFILGAGTMGSGIAQAFAQKGYEVVLRDIKDEFVDKGLAVIN
                     KNLSRLAEKGKITPEAKEEILSRISGTTDMNLAEDCDLVVEAAVENMEIKKKIFAELDN
                     ICKPEAILASNTSSLSITEVASATNRADKVIGMHFFNPAPVMKLVEVIRGMATSQETFD
                     KVKELSLAIGKDPVEVAEAPGFVVNRILIPMINEAVGIFAEGIASAEDIDKAMMLGANH
                     PMGPLALGDLVGLDVCLAIMDVLFKETGDNKYRAHSLLRKYVRAGWLGRKTKKGFYDYS
                     R"
     CDS             complement(926..1936)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 179.7;
                     E-value: 1.7e-55)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg92_2"
                     /sec_met_domain="ETF (E-value: 4.1e-56, bitscore: 179.7,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 2.3e-39, bitscore:
                     123.4, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIADYKGVWVFAEQREGELQKVSLELLGEGRKIADKLGVKLTAL
                     LLGDKIEALSETLAAYGADQVLIAENPLLSHYTTDGYTKVICDLVNERKPEILFIGATF
                     IGRDLGPRVAARLSTGLTADCTSLDIDEEGRYLLATRPAFGGNLMATIACSEHRPQMAT
                     VRPGVFEKLDKNNKNSASIEKVEVSLNESDVRTEILKIVKQKKDIADIGEANIIVSGGR
                     GVGEKESFKLLQDLAEVMGGTVAASRAAVENGWLERDYQVGQTGKTVRPTIYIACGISG
                     AIQHLAGMQDSDYIIAINKDETAPIMKVADLAIVGDLNKVIPEIITQVKAMKEEA"
     CDS             complement(1954..2736)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 165.8;
                     E-value: 3.1e-51)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg92_3"
                     /sec_met_domain="ETF (E-value: 7.6e-52, bitscore: 165.8,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIIVCIKQVPDTTAVRIDPKTGTLIREGVPSIINPEDKNALEEA
                     LVLKDKYGASITVISMGPLQAKDALREALAMGADDAVLVTDRAFAGADTLATSKALAGV
                     INKLGDFDIIFAGRQAIDGDTAQVGPEIAEHLQIPQVTYVQEVKVEGDELHINRALEDG
                     YEIIKVKSPVLLTAIKELNAPRYMHAQYIFDSADKEIKIMTADDIDVDKSELGLSGSPT
                     KVKKSMTKEAKGQGEVINMPVQEAVSYLLKKMKEKHYI"
     CDS             complement(2749..3888)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg92_4"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.1e-56, bitscore:
                     181.2, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 7.3e-29, bitscore:
                     90.0, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.9e-38, bitscore:
                     120.7, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFALSKEQELVKQMVREFTINEVKPIAAEIDETERFPMETVKKM
                     AKYGLMGLPFSKEYGGAGGDTLSYILAVEELSKACGTTGVILSAHVSLCASLIESFGTE
                     AQKQKYLVPLAKGEKIGAFGLTEPNAGTDAAGQQTVAYLDGDNYVLNGSKIFITNGGVA
                     DVFIIFAMTDRSKGTKGISAFIVEKNFPGFSIGKVENKLGIRASSTTELIMENCIVPKE
                     NLIGQEGKGFGIAMKTLDGGRIGIAAQALGIAEGALEEAVHYMKERKQFGKPLSAFQGL
                     QWMVADMDVKIESARYLVYKAAWLKENKMPYSVDAARAKLYAAEVAMEVTTKAVQLFGG
                     YGYTKEYPVERMMRDAKITEIYEGTSQVQKMVIAGSVLR"
     CDS             complement(3910..4695)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: EC1_2-faecali"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg92_5"
                     /sec_met_domain="EC1_2-faecali (E-value: 1.3e-90, bitscore:
                     293.1, seeds: 67, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELKNVLLEKEENIAVVTINRPKALNALNSDTLKELEWVIADLEE
                     DDSIYAVILTGAGGKAFVAGADITEMKDLNALQGRKFSILGNRVFRRLELLEKPVIAAI
                     PGFALGGGCELAMACDIRIASEKAKFGQPETGLGITPGFGGTQRLARIVGMGRAKELIY
                     TGSVIKADEAYRIGLVNKVTTVEELMNEAKAMASAIAKQAPIAVKLSKAAINRGMQVDI
                     DTALSYEAEAFGECFSTEDQKEGMTAFVEKKSEKNFQNK"
     CDS             complement(4927..5559)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CoA_binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg92_6"
                     /sec_met_domain="CoA_binding (E-value: 3.8e-28, bitscore:
                     88.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRKKNISMAVIKRLPKYYRYLMELLKNDVDRISSKELSEKIGFT
                     ASQIRQDLNCFGDFGQQGYGYNVKELYNQISGILGLQKDYNTVIIGAGNLGQAIANYTR
                     FNKLGFNLKGIFDANPKLVGIRIRDVDIADIDEMPEFLKHNSIDIGIICVPQTNAQKVC
                     DMLVEGGVKGIWNFAPIDLAVPEGVITENVHLSESLLTLVYLLNEGN"
     CDS             complement(5812..6405)
                     /locus_tag="ctg92_7"
                     /transl_table=11
                     /translation="MSTLSIFLMAFGLAMDAMIVSLSLGVRSCRENKVTIGIKAGLYFG
                     LFQGIMPLIGWIMGKNFAKYITSIDHWVAFTLLSLIGGKMIIDAYKEKEEDSCVMSFST
                     KLYLTLAVATSIDALAVGLSLALVNINILTPVFFIGGVTFILSFLAVIAGEKLGSLFKN
                     KAAVLGGIILIFIGSRILAEHLEIGEILLGIINR"
     CDS             complement(6653..7849)
                     /locus_tag="ctg92_8"
                     /transl_table=11
                     /translation="MLLFEVLSKSNNGIEVLHKRSIYPKADVTYISPDTIKEEIKDIES
                     SSHIQGINMELDRSDINLRLEGFEYVNISSLKYKYADVPKLEVFGSGTKVKMERISVDS
                     LYLSNCSAFIIESHINEIMFTGDKGIKNIYDLNLDLLKSHLGEVKVRAACKRIHIQSCQ
                     VNELICLEEVNKIERIIIWQNSTLEKIALDGEIEKVKLINCNIEELRFNNKCRVREIST
                     LYSYIERIHRCFYSTIGNKNMDAWMLMKQSAKYDNRPHDLAEAGYNYSRLERKTVLKGA
                     QKSVAFIIEYTSGYGYRPMRTIVTGGLIWIIFGILYWILTISGLDGIVNSSQGSLQGGW
                     ASLMYSMYFSIITLSTTGYGEIVPLGWIVRMLSGIESVLGVLIMSLFIFTLTKKYGSL"
     CDS             complement(7964..9034)
                     /locus_tag="ctg92_9"
                     /transl_table=11
                     /translation="LLYALILAGGKGTRLYPLSRSDTPKQFLKVVNNKSFLVNTVERIM
                     PIVEANNIYIVTNRDYLEKIREELPYISQENIFVEPANKETATCIGLSAVKLLKRDKDA
                     VMMVLPSDHYIEDEKEFQETLMQAVEVAERRRGIVTIGVTPTAPETGYGYIEMGERTQG
                     NIATYKVARFTEKPNIEVAKDFLLKGTYLWNSGMFIFRADVIIREFEKYLPKIYRSLME
                     IYQALDTPQESSIIEDQYKLIDGISIDFGIMQKTRKAFVIKCDFIWDDIGSFSALNRFL
                     KKYGGNFVWDNVFLDQSENCSVFGNKENLIIGFGIKDLVIVNAKDVILIMDKCKDQEIK
                     HLINELSEYSDFKKYI"
     CDS             complement(9222..10481)
                     /locus_tag="ctg92_10"
                     /transl_table=11
                     /translation="MKEEVSIISKIKEWLFNNMVTILFVVLCAAGIEVSRQPLSFVLGE
                     LVTRIARNLFLVLSLIIPVLAGMGLNFGIVIGAMAGQIAIIAVTHWKIAGIGGFLLCIL
                     ISTPIAILFGYFTGKLLNKTKGQEMIASMILALFANGLYQLLFLFFVGTIIPMDNPVMV
                     LSGGIGIKNTLDLTGGIKYTLDSLFEMDLFVTIMGLLAAAILYNLIKMLINYRRTKKTG
                     GKYLFNLLIIIAAMLCSILIPKLNFAFKFVNVPIITLFVIGAVALFNSFLYRTKLGQDL
                     RTVGQDMHVANASGIKVNKVRIIAIVISTVLAAWGQLIFLQNIGTLNTYGSHEQVGMFS
                     IAALLIGGASVSKATNGQALLGVILFHTLFIISPTAGKNLFDNPQIGEYFRGFIAYGVI
                     GVSLGIYAWKKSVETRRLIE"
     CDS             complement(10478..11521)
                     /locus_tag="ctg92_11"
                     /transl_table=11
                     /translation="MQDKLKSWYGVIGLPRLIIIGFFILLCILSVVLGLPINMLISDTL
                     VRTGMNGILVLAMVPGILCGIGLNFGLPLGILCGLLGGLISIEMDLTGLTALFTAILIS
                     IPIALIVGYGYGVLLNKVKGSEMMVSTYMGFSSVSLMCIAWLILPFNSIEIKWPIGQGL
                     RTTIALADRYDKVLDNALSFYIGGVRFPTGLIVMFLLCCLIMWIILKTKKGMAMIAAGS
                     NEKFALASGINIQKSRIFGTVLSTILGAVGIIVYSQSYGFYQLYQAPMMMGFAAVAAVL
                     IGGASATKAKISHVIIGTLLFQGLLTIGLPVANKIVTEGNLAEVVRIIISNGIILYALT
                     KVGGSEE"
     CDS             complement(11524..13125)
                     /locus_tag="ctg92_12"
                     /transl_table=11
                     /translation="LQKADVLEMKNIEKEYFGNKVLKNVSLKIRRGEIHALLGENGAGK
                     STLMNILFGMPVIHNTGGFKGQVLFQGKEAHIKSPMEAMKSGIGMVHQEFMLIPGFTIS
                     ENIKLNREITRHNLVSRLLGGSLKSLDYKAMDSESREALNTAGLNVEEYVKVEGLPVGY
                     MQFIEIAREIDKKNISLLVFDEPTAVLTESEAEKLMEAMERLASKGIAIIFITHRLDEV
                     MKVADEITILRDGEVVAARKSSETNILELAELMVGRKIERTETDGISKVEKKDIILKIE
                     NLKVNMPGERVRDINLDIYKGEILGIGGLAGQGKIGIANGIMGLFQSSGKVTMEGKEIP
                     LNNPQEVLKQGIAFVSEDRRGVGLLLEDSIENNIAITSMCINNAFLKNYLLFTQSNRRK
                     IREHALKMIKDLDIRCTGPHQEVKRLSGGNQQKVSIARALTLKPKILFVSEPTRGIDVG
                     AKKLVLDLLTALNKEFNMTIIMTSSELAELRSICERIAIVSEGKISGILKPESSDGEYG
                     LLLSGHAADALIGKEE"
     CDS             complement(13208..14350)
                     /locus_tag="ctg92_13"
                     /transl_table=11
                     /translation="MFKKAAAFFLSGIFALSLVGCSGGGKDNPGQNKKFKIGVLTGTVS
                     QNEEEYRAAEKMKKKYGDMIVLQTYPDNFTKEQETTIANALSLAADPDVKAIVFVQAVP
                     GASAAIDKVREKRKDMLFIAGVPGEDPDMIASKADIVLQADELGMGTTIIEQAKKLGAK
                     KFVHYSFPRHMSYQMLAMRRDILKDTCSKLGLEFVDATAPDPTGDAGVPGAQQFILEDV
                     PRKVKELGKDTAFFSTNCAMQEPLIKAVLQEGAIFPQQCCPSPYHGYPGALGISIPDDK
                     KGDLEYIVKEESRIIAEKGGKGRFSTWPVPINMMFVEGGVEYAKAYLEGNTKERNDKAK
                     LEEIFTNISKKPIKLTTLDDEKTKKKLDNFYMLLSEYITF"
ORIGIN
        1 gttattggta atttctatct tgaataatcg taaaagcctt ttttggtctt tcttcctaac
       61 cagcctgctc ttacatattt tctaagaaga ctgtgagctc tgtatttgtt gtctcctgtt
      121 tctttaaata aaacatccat tatagcaaga cacacatcca atcccacaag gtctcctaaa
      181 gctaatggtc ccataggatg gtttgctccc agcatcatag ctttatctat atcctcagcg
      241 cttgctatac cttctgcaaa aattcctaca gcttcattta tcataggtat taatatcctg
      301 ttcacaacaa acccaggagc ttcagcaact tcaacaggat cttttccaat agccagggat
      361 aattccttaa ccttatcaaa ggtttcctgt gatgtagcca ttcctcttat aacctcaaca
      421 agcttcatta caggagcagg attaaagaaa tgcattccta taaccttatc cgctctgtta
      481 gtagctgaag caacttctgt tatagataat gatgaagtat ttgatgccaa aatcgcttca
      541 ggcttgcaga tgttatccag ttccgcaaat atcttctttt tgatttccat gttttccacc
      601 gctgcttcta ctacaagatc gcagtcctca gctaaattca tatcagtagt accgctgatt
      661 cttgatagaa tttcctcttt agcctctggt gttatctttc ctttttcagc taacctgctt
      721 aagtttttat ttataacagc aagtccttta tcaacaaatt catcctttat atcccttaaa
      781 accacttcat atcctttttg tgcaaaagct tgagcgattc ctgagcccat ggttcctgct
      841 ccaagaataa atatcttttc catataataa acctccatta ttaagaaaat attatattca
      901 ataaattacc atttcattat aactcttatg cttcctcttt catagcctta acttgagtta
      961 taatttcagg tattactttg tttaagtctc ctactatagc taagtcagca accttcatta
     1021 taggagctgt ctcatcttta tttattgcta taatatagtc tgaatcctgc attcctgcta
     1081 aatgctgaat tgctccggat ataccgcagg ctatatatat agtaggtcta actgtctttc
     1141 ctgtttgccc aacttgataa tctctttcca gccatccatt ttccaccgca gctcttgaag
     1201 cagctacagt tcctcccata acttcagcta aatcctgaag gagcttaaag ctttcctttt
     1261 ctcctactcc tcttcctcct gaaacgatta tgttagcttc tcctatgtct gctatatcct
     1321 ttttctgctt cacaatcttt aagatttcag tgcgtacatc actttcattt aaagatactt
     1381 ctaccttttc tatagaagcg gaatttttat tatttttatc aagcttttca aatactccag
     1441 gtctcactgt agccatttga ggtctatgtt ccgagcaggc tatggttgcc attaagtttc
     1501 ctccaaaggc tggtcttgtt gccagaaggt atctgccttc ttcatctata tctaatgaag
     1561 tacaatctgc agttaatccc gtagaaagtc ttgcagcaac tctaggccct aaatccctgc
     1621 ctataaatgt cgctcctata aataatattt caggctttct ttcattaact aaatcacata
     1681 ttaccttagt atatccatct gtagtatagt gagaaagcag cggattttcc gcaataagca
     1741 cctggtcagc accgtaggct gccaatgttt ctgataaagc ttctatttta tcacctaata
     1801 acagtgcagt aagcttaaca ccaagcttat cagctatttt tcttccttct cccaaaagct
     1861 ccagagaaac cttttgaagc tctccttctc tttgctcagc aaaaacccaa acgcctttgt
     1921 aatcagctat attcatttac tttacctcct aacttagatg tagtgttttt ccttcatttt
     1981 tttaagcaga tagcttactg cttcctgaac aggcatatta ataacctctc cctgtccttt
     2041 agcttccttt gtcatagact ttttaacctt tgtaggcgaa cctgaaaggc ctaattctga
     2101 tttatctaca tctatgtcat ctgcagtcat aatttttatc tctttatcag cagaatcgaa
     2161 gatatactgt gcatgcatat atcttggagc atttaattct tttatagctg taagcagcac
     2221 tggagattta accttgatta tttcatatcc atcctctaat gctctgttaa tatgaagttc
     2281 gtccccttca accttaactt cctgaacata ggttacctga ggaatttgta ggtgctcagc
     2341 aatttcaggt cctacttgag cagtgtcgcc gtcaatagcc tgtctacctg caaagataat
     2401 atcaaaatca cctaatttat ttataacccc agccagtgct ttagaggttg ccaaggtgtc
     2461 agctcctgca aaggctctgt cagtaactaa aacagcatca tcagctccca tagctaaagc
     2521 ttctcttaaa gcatccttag cctggagcgg tcccatactt attacagtta ttgaagctcc
     2581 atacttatcc ttaagcacta aagcctcttc caaagcattc ttatcttcag gattaattat
     2641 ggatggaact ccttctctta ttaaagttcc tgtttttgga tctatcctaa cagcagtagt
     2701 atccggtacc tgttttatac aaactataat cttcattaaa ctgcctcctt atctcaaaac
     2761 actaccagcg ataaccattt tttgaacctg tgaagttcct tcatagattt cagttatctt
     2821 agcatctctc atcattcttt ctactggata ttccttggta taaccgtatc ctccaaagag
     2881 ctgaacagct tttgtagtta cttccatagc aacctctgca gcataaagct ttgctctggc
     2941 tgcatctaca gagtagggca ttttattttc tttaagccat gctgctttat ataccaaata
     3001 tcttgctgac tcaattttaa cgtccatatc agcaaccatc cactgaagtc cttggaatgc
     3061 agaaagaggc tttccaaact gttttctttc cttcatataa tgaacagctt cttcaagagc
     3121 tccttcagct atgcccaaag cttgagcagc tataccaatc cttccgccat ccagggtttt
     3181 cattgctatt ccgaagcctt ttccttcctg tccaataaga ttttccttag gtacaatgca
     3241 attttccatt ataagctctg tggtagatga agctctgata ccaagcttat tttcaacctt
     3301 acctatggag aatccaggaa aattcttttc tacgatgaag gcgcttatgc ctttagttcc
     3361 tttgcttctg tctgtcattg caaatattat aaatacgtct gcaactcctc cgttagttat
     3421 aaatatctta gagccattta acacatagtt atctccatca agataagcaa cggtctgctg
     3481 accagcagca tcagttccgg cattaggctc agtaagacca aaagccccaa tcttttctcc
     3541 cttggcaaga ggaacgaggt atttctgttt ctgagcttct gttccaaagc tttcaattaa
     3601 ggatgcacat agcgaaacgt gagctgataa tataacacca gtagtaccgc aggcttttga
     3661 aagctcctcc accgctagta tataagatag agtatcgcca cctgctccac cgtactcctt
     3721 tgaaaatgga agtcccataa gaccgtactt ggccattttt ttcactgttt ccattgggaa
     3781 tctttcagtt tcgtctattt cagctgctat aggcttaact tcattaatgg taaactctct
     3841 taccatctgt tttacaagtt cttgttcttt agataaagca aaattcattt attttacctc
     3901 cttgatatgc tatttatttt ggaagttttt ttctgatttc ttttctacaa aggctgtcat
     3961 accttctttt tgatcttctg ttgagaaaca ctctccaaaa gcttcagctt catagcttaa
     4021 tgcagtatca atgtccacct gcattcctct atttatcgct gctttgctta gctttacagc
     4081 tataggtgct tgtttagcta tagcactagc catagctttt gcttcattca ttaattcttc
     4141 aacagttgtt accttattta ctaaaccgat tctataggcc tcatccgctt taattacaga
     4201 gcctgtataa atcagttcct tagctcttcc cattccaact attctggcaa gtctctgagt
     4261 tcctccaaag cccggtgtga ttcccagacc tgtttcaggt tgtccaaatt tagctttttc
     4321 tgaagctatc cttatatcac atgccatggc aagttcgcag ccaccgccaa gtgcaaaacc
     4381 aggtatagct gcaattactg gtttttctag tagttctaat cttctgaaaa ctctatttcc
     4441 aagaatagag aattttcttc cctgcagggc atttaaatcc ttcatttcag ttatatctgc
     4501 tcctgcaaca aaagcttttc caccagctcc ggtcaatata actgcataaa tactgtcatc
     4561 ctcttctaaa tctgcaatta cccattcaag ttcctttaat gtatcgctgt ttaaagcatt
     4621 taaagcctta ggtctgttaa ttgtcaccac agcgatgttt tcttcttttt caagaagaac
     4681 attttttaat tccatagttg tatcctcctt aatttatagc tttaagaaaa atattgttta
     4741 aatcataggc tccaggataa tttcgctttt tctccttaaa ccctattatt tatttatggt
     4801 tattaaagtt aaataatgtt aatttgttca aaaacaaaaa aaataattga agcgttaact
     4861 tcaattatta ttatataact ttgttaaatt ttttgcaatc atttattcaa attaatattg
     4921 aataatttaa tttccttcat ttaataggta aacaagtgtc aacagacttt cacttaaatg
     4981 tacattttca gttataacgc cttcaggaac agccaaatct attggggcaa agttccagat
     5041 tcctttaact cctccctcta caagcatatc gcataccttc tgagcattag tttgaggcac
     5101 acagattatt cctatgtcaa tactgttatg cttaagaaac tctggcattt cgtctatatc
     5161 agcaatatcc acatccctta ttctaattcc tacaagcttt ggatttgcat cgaatatacc
     5221 ttttaaatta aatcctaatt tattaaatct ggtatagtta gcaatggcct gacctaggtt
     5281 tcctgcacct attatcactg tgttatagtc cttttgaaga ccaagtatac cgctgatctg
     5341 attgtaaagc tcctttacgt tataaccgta tccctgctgt ccaaaatctc caaagcagtt
     5401 aagatcctgt ctgatttgag aggcggtaaa accaatcttt tcgcttaatt ccttagatga
     5461 aattctatcc acgtcatttt ttaatagttc cattaaatat ctgtaatact taggcagtct
     5521 tttaataact gccatagaaa tatttttctt tctatccata tatagcacac ctcttctgta
     5581 taattcttaa ttttaaattt tccactgaaa ttaatatata atttatataa tatcatagta
     5641 tatattatat aatctatatt ttattattaa ttatacttaa ttgataaata ataagcaatt
     5701 aattttattt catctcccaa aactttgtta attatataac tatataatat ggcttatatg
     5761 tattttttat gtataaattc tatattttta ccaatccact ttttcattac tctatctgtt
     5821 aattatacct aataatattt caccgatttc taaatgctca gctaatatcc tgctgcctat
     5881 aaaaatcagt attatgcctc caagcacagc agctttattc ttaaataagc ttccaagctt
     5941 ttcaccggct attactgcca aaaagctgag tataaaagtt actccaccta tgaaaaaaac
     6001 cggtgttaat atgtttatat taactaaagc aaggctcagc cctacagcca aagcatctat
     6061 actggtagct actgcgaggg ttaagtaaag ttttgtgcta aagctcatta cgcaggaatc
     6121 ttcctctttt tctttataag catcaataat catcttgcct ccaatcagtg aaagcagtgt
     6181 aaaagccacc caatggtcaa tagaagtaat gtacttagca aaatttttcc ccattatcca
     6241 tcctattaga ggcattatcc cctgaaataa cccaaaatat aacccagctt ttattccaat
     6301 ggttacttta ttctctctgc agctcctcac tcctaaactt agagaaacta tcatagcatc
     6361 catggccaaa ccaaaggcca ttaaaaatat tgataatgta ctcatttttg acccccaagt
     6421 taaattattt tatctttaat ttttcaacaa aaaagagact tatgtataac gcaacccttc
     6481 gttatacata agtctcactt tttaatacaa agccagatgt aaacatcagt atgttgactc
     6541 tgtaacaagg caattacaag ccttgcaagc tactccctta tgcctgaaag taaatctcaa
     6601 cttcttcatt atagagaagt tataataagc tgtcaatatt ctcttaaata gtttataaag
     6661 aaccatattt tttggttaga gtaaaaataa acagggacat aataagcacc ccaagcactg
     6721 attcaattcc tgaaagcatc ctcacaatcc atcctaaagg tactatttcc ccataccctg
     6781 tagtagacag ggttattatt gaaaaataca tactatacat caaactggcc catccaccct
     6841 gtaaggatcc ttgtgagcta ttcactatcc catctaaccc gcttattgta agaatccagt
     6901 atagtatccc aaagatgatc catataagtc ctcctgtaac tatagtcctc attggtctat
     6961 atccgtaacc tgaagtatat tctattatga aagccacact tttttgagca ccctttaata
     7021 ctgtttttct ttccagccta gaataattgt aacctgcttc agctaaatca tgaggcctgt
     7081 tatcatattt tgccgactgt ttcataagca tccatgcatc catattttta tttcctatgg
     7141 tgctgtaaaa gcatctgtga atcctttcaa tataggaata taaagtagaa atctctctta
     7201 ccctgcattt attattaaat ctgagttcct caatattgca gtttataagc tttacctttt
     7261 caatttctcc atctaaagca atcttttcca gggtagagtt ctgccatatt attattcttt
     7321 ctatcttatt tacttcttca aggcagataa gctcattaac ctgacagctt tgaatatgta
     7381 tccttttaca ggctgctctt acttttactt ctcccagatg agacttcaaa aggtcaaggt
     7441 ttaagtcata gatattttta atccctttat cccctgtaaa cataatttca ttaatatgag
     7501 actcgattat aaaagcgctg cagttggata aatacaagct atctacagat attctttcca
     7561 ttttgacctt tgtccctgaa ccaaatacct ctaatttagg aacatcagca tatttatact
     7621 ttaaagagct gatatttaca tattcaaatc cttctaatct aagatttata tcacttctgt
     7681 ccagttccat atttatcccc tgtatatgtg acgatgattc tatatccttt atttcttcct
     7741 ttatagtatc aggacttata taggttacat ccgctttagg ataaatactc ctcttatgga
     7801 gtacttctat tccattattc gatttgctta aaacttcaaa aagtaacata atcctctcct
     7861 gtattgttct agtatttata taattataac aaatacctct cttatatttc tgtaaacaaa
     7921 ttgaactgtt taataacatt cattaaacag ttcatcaata ttactatata tactttttaa
     7981 aatccgagta ttcacttaat tcatttataa gatgcttaat ctcctgatcc ttacatttgt
     8041 ccattattag tatcacatcc ttggcattta caatgaccaa atctttaatt ccaaaaccta
     8101 ttataaggtt ttccttattc ccaaatacag aacagttttc actttgatcc aaaaacacat
     8161 tatcccaaac aaaatttcct ccgtactttt ttaaaaatct gtttaaggca gaaaagcttc
     8221 ctatgtcatc ccatatgaaa tcgcacttaa tcacaaatgc ctttcttgtc ttctgcatta
     8281 ttccaaagtc tatggatatt ccatctataa gcttatattg gtcttctatg atactgcttt
     8341 cctgaggagt atccagagcc tgataaatct ccattaagct tctatatatc tttggaaggt
     8401 acttttcaaa ttcccttata atcacatctg cccggaaaat aaacattccg ctgttccaaa
     8461 gataggttcc cttaagcaaa aaatcttttg ctacttctat attaggtttt tcagtaaatc
     8521 tggccacctt gtaggttgca atgtttccct gagttctttc ccccatctca atatagccgt
     8581 agcctgtttc cggtgcagta ggtgtaactc caatggtaac tatcccccgc cttctttccg
     8641 ctacctccac tgcctgcata agcgtttcct gaaactcctt ttcgtcttct atataatgat
     8701 ccgaaggcag caccatcatc accgcatcct tatctctttt taaaagctta actgcagaaa
     8761 gacctataca ggtagcagtc tctttattag caggctctac aaaaatattt tcctgggaaa
     8821 tgtaaggcag ttcctccctt atcttttcta aataatctct atttgtaact atataaatat
     8881 tatttgcttc tactataggc attatccttt ctacagtgtt tactagaaaa cttttattat
     8941 ttactacctt aagaaattgc ttaggggtat cgcttcttga aagaggataa agtctcgttc
     9001 cttttcctcc tgctaatata agcgcataaa gcaaaggtct tcactccata aaataactat
     9061 gatttataat atgtgcatgt aatgatagtt gttaaaggat aattaaaaat tataaatgaa
     9121 gaaaaaaaac tcctggcgga gttttttaat aagcataatt caaagaaatt ccggaggaat
     9181 tttctccttc tttttgcatt ttaattttat gtctttaatt attactcaat caatcttctg
     9241 gtctccacgc ttttcttcca ggcataaatt cccagtgata ctcctattac accataggct
     9301 ataaagcctc tgaaatactc tcctatctga ggattgtcaa acaaattttt ccctgcggtt
     9361 ggagatatta taaatagggt gtgaaatagt ataacaccta aaagtgcttg tccattggta
     9421 gctttggata ctgaggctcc tccaattagc agtgcagcta tagaaaacat ccctacctgc
     9481 tcatgactgc cgtaggtgtt tagcgttcct atgttttgaa ggaatataag ctgtccccaa
     9541 gctgccaata ccgtagatat gactatggct ataatcctta ccttgtttac ttttatccct
     9601 gaagcgttag caacatgcat atcctgccct acagttctaa ggtcctgacc gagctttgtt
     9661 ctgtaaagga agctgttgaa tagagctact gctcctatga caaataaggt gattatcgga
     9721 acatttacaa atttgaaagc gaaattcagt ttaggtatca gtatggagca caacatagct
     9781 gctataatta taagcagatt aaataaatat tttcctcctg tttttttagt ccttctgtaa
     9841 tttatcagca tttttattaa attgtagaga attgctgctg cgagaagtcc cattatggtt
     9901 acgaacaagt ccatttcaaa caagctgtcc agggtgtatt taattccccc ggtaaggtct
     9961 aaggtatttt ttatacctat tcctccgctg agaaccatta caggattgtc cattggaatt
    10021 atagttccta caaagaagag gaataaaagc tggtaaagtc catttgcaaa aagggctagt
    10081 atcatgctgg ctatcatttc ctgccctttt gttttattga gaagctttcc ggtaaaatat
    10141 ccaaacaata ttgctatagg tgtacttatt agaatacata ggagaaatcc gcctattcct
    10201 gcaatcttcc agtgggtaac cgctatgatt gctatttgtc ctgccatggc acctatgacg
    10261 attccaaaat tcaaacccat tcccgctaat accggaatga ttaaggataa aaccaaaaac
    10321 agattccttg caatccttgt taccaattcc cctaaaacga aggaaagtgg ctgccttgaa
    10381 acttcaatgc ccgcagcaca taatactacg aataaaatag taaccatatt attaaataac
    10441 cattctttta ttttagagat aatacttact tcctctttca ttcctcacta cctcctactt
    10501 tggttaatgc atatagtatt attccgttgc ttatgattat ccttactacc tcagccaggt
    10561 taccctcagt aactatttta ttagctactg gaagacctat ggtgagaagt ccctgaaaca
    10621 ataaagttcc tataataacg tgggatattt ttgcctttgt ggctgaagca cctccgataa
    10681 gcacagcggc tacagcagca aaccccatca tcataggggc ctgataaagc tggtagaagc
    10741 catagctttg agaataaact ataatcccta ctgctcccag tatggtggaa agcaccgttc
    10801 cgaatatcct gctcttttga atattaattc ctgatgccaa agcaaatttc tcattacttc
    10861 ctgcagcaat catagccatg ccttttttag tctttaaaat tatccacatt atcaagcagc
    10921 ataataaaaa catcactatt aaccctgtag gaaatctaac tcctcctata taaaagctta
    10981 aggcattgtc cagaacctta tcatatctgt cagcaagagc aatggtggtc cttaatccct
    11041 gtcctatagg ccattttatt tctatgctgt taaagggaag tataagccaa gctatacaca
    11101 ttaaagatac tgaggaaaat cccatataag tagataccat catttctgac cctttaacct
    11161 tatttaataa aactccataa ccgtatccta caataagtgc tatgggtata gaaatcagga
    11221 tagctgtaaa cagtgcagta agacctgtaa gatccatctc tatgcttatc agtcctccta
    11281 aaagtccgca taatatcccc aaaggaagtc cgaagtttaa tcctattcca cagagaatcc
    11341 ccggcaccat ggcaagaacc agtattccat tcattcctgt acgcaccaga gtatctgaaa
    11401 taagcatatt tataggcagt cctaaaacta cggaaagaat gcataacaaa ataaaaaatc
    11461 caattataat aagtcgggga agtccgatga ctccatacca gctttttagt ttatcctgca
    11521 taattattcc tccttcccta ttaatgcatc agcagcatgt cctgacagca gcagtccata
    11581 ctccccatcg ctggattcag gctttaatat tcctgatatt ttaccttcag atacaatagc
    11641 tatcctttca catatggatc taagctcagc cagctcagaa gaagtcatta taatcgtcat
    11701 attaaactcc ttattaaggg cagtgagaag gtccagcaca agctttttag ctccgacgtc
    11761 tattcctctg gtaggttcgg aaacaaagag tattttgggt tttaaggtta acgctctagc
    11821 gatactgacc ttctgctgat tccctccgct aagcctttta acctcctgat gaggacctgt
    11881 acaccttata tctaaatcct ttatcatttt taaggcatgc tctcttattt tccttctatt
    11941 actttgtgta aataacagat aattttttaa aaatgcatta tttatgcaca tagaagttat
    12001 agctatattg ttttctatgc tgtcttcaag gagaagtccc actccccttc tgtcttccga
    12061 cacaaaagct ataccctgtt ttaaaacttc ttgcggattg ttaagaggaa tctctttacc
    12121 ttccatagta actttgccgg aactctggaa tagtcccatt ataccgttag ctatccctat
    12181 tttcccctgg cctgcaagcc cgccaatacc cagtatttct cctttgtata tatctaaatt
    12241 tatatccctc accctttccc cgggcatatt gactttcagg ttttctattt taagtattat
    12301 atctttcttc tcaaccttac tgattccatc agtttccgtc ctttcaattt ttctgccaac
    12361 cattaattca gccagctcaa ggatatttgt ttcactgctc ttccttgctg ccaccacctc
    12421 accgtcccga agtatggtta tctcatccgc tactttcata acctcatcta atctatgagt
    12481 tataaatatt atagctatcc cttttgaagc aagtctctcc attgcctcca ttagcttttc
    12541 cgcttcactt tccgtcagca cagcagtagg ctcgtcaaaa actaaaagac tgatattttt
    12601 tttatctatt tctctggcta tttctataaa ctgcatatat ccaacaggaa gtccttccac
    12661 ctttacatac tcctccacat tcaatcctgc agtgtttaat gcttccctgc tttcactatc
    12721 catagcctta taatccaggc tttttaaaga acctccaagt aatctgctta ctaaattgtg
    12781 tctggttatc tccctgttga gctttatatt ttcagatatt gtaaagccag gaataagcat
    12841 aaattcctga tgcaccatgc ctattccgga tttcattgcc tccatagggc tctttatatg
    12901 tgcttctttt ccctgaaaca atacttgtcc tttaaaacct cctgtattgt gaattacagg
    12961 cattccaaag aggatgttca taagggtgga tttccctgct ccattttctc ccaaaagagc
    13021 atggatttct ccccttctaa tctttagaga aacattttta agtactttat tgccgaaata
    13081 ctccttttca atgtttttca tttcaagaac atcagctttc tgcaaaattc tcccccctta
    13141 tttcataatg ctaaggagtg aagcccagcc tcactcccat aaactaaatc aaattaagat
    13201 aaaaatatta gaatgttata tattctgaaa gcagcatata aaagttgtcc agcttcttct
    13261 ttgtcttttc atcatctaaa gttgtgagtt ttataggttt ctttgatata ttagtgaaga
    13321 tttcctcaag cttggctttg tcatttcttt cttttgtatt tccttctaaa taagctttgg
    13381 catattccac tccgccttct acaaacatca tatttatagg tacaggccaa gtagaaaatc
    13441 ttccctttcc gcctttttca gcaattattc tgctttcttc cttaactata tactctaaat
    13501 ctcccttctt atcatcaggg atacttattc ccagtgcgcc aggatatcca tggtatggtg
    13561 atggacaaca ctgctgagga aatatagccc cctcctgaag cactgctttt attaggggtt
    13621 cctgcatagc acagtttgta ctaaaaaatg ctgtatcttt tccaagctct ttaacttttc
    13681 ttggaacatc ctcaagtata aactgctgtg cccctggaac tcctgcatca cctgtagggt
    13741 ccggtgcagt tgcatctaca aattcaagtc ctaatttgct gcaggtatct tttagaatat
    13801 cccttctcat agcaagcatt tgataggaca tatgtcttgg aaaggaataa tgaacaaact
    13861 tctttgctcc taattttttc gcctgctcaa ttatggttgt acccattcct aattcatccg
    13921 cctgaagtac tatatcagct tttgatgcta tcatgtcagg atcctctccg ggaacccccg
    13981 ctatgaacag catatcttta cgtttttctc taaccttgtc tatagctgct gaagcaccgg
    14041 gtactgcctg tacaaaaact atagccttaa catcaggatc tgcagctaag cttaaagcat
    14101 tagctattgt agtttcctgc tctttagtaa aattatcagg ataggtttga agcactatca
    14161 tgtctccata cttctttttc atcttttctg ccgctctata ctcctcttca ttttgtgaaa
    14221 cagtacctgt taatactcca attttgaatt tcttattttg cccagggtta tctttaccac
    14281 caccgctgca tccaactaaa ctcaaagcaa aaattccaga aagaaaaaac gctgctgctt
    14341 ttttaaacat tacaatcccc cttaattaat tactataaaa atcctcacaa atttatataa
    14401 taaataatat tacttttgca aggaatatat tcttgtttat ttaaaatatg ggttagggtg
    14461 ttataattct gctttatata gaatctgtct atgtgaaaat ctgaatttca taccttcatt
    14521 taaatgcaaa aatccagaaa cccatggcaa ggagtttctg gacactttat taacctttat
    14581 atttattctg ttttaacatt ttaattaaac ctgaaattat ctattatcaa ctgaagctct
    14641 tctgccttaa tatttaagct ttcagacagt tcatgtattt ttttattttc ttccg
//
