LOCUS       c00075_NZ_CABK..        3256 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CABKTP010000075.1 Clostridium polynesiense isolate
            MGYG-HGUT-01479, whole genome shotgun sequence.
ACCESSION   c00075_NZ_CABK..
VERSION     c00075_NZ_CABK..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-03-14 01:45:19
            Original ID  :: NZ_CABKTP010000075.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3256
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..156
                     /locus_tag="ctg75_1"
                     /transl_table=11
                     /translation="SYICDVCGYVYDPAVGDEDNGINPGTSFEELPEDWVCPLCGVGKD
                     QFSPVE"
     protocluster    1..3256
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[185:3191]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      186..3191
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3256
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3256
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(186..908)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 78; E-value:
                     2.7e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg75_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.5e-31, bitscore:
                     95.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LLGKIHSIETMGLVDGPGVRVVVFFQGCSLRCAFCHNPDTWGANE
                     GEEITSQDLLKKILRYKTYFKSSGGGITFSGGEPLLQPDFLIEMLKLCRENGIHTTIDT
                     AGCGIGKYSEILKYTDLVLFDIKHIDAEEYKKMTRNNINESLKFLEAVQQSGNKMWIRQ
                     VIIPNLNDSREYIKGLADIINTLNNVEKVELLPYHLLGVSKYEALGKKYRLEGVPEMDK
                     EKNKELEDYLKTLISKKI"
     CDS             complement(963..3191)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg75_3"
                     /sec_met_domain="Gly_radical (E-value: 7.1e-46, bitscore:
                     143.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 0.0, bitscore:
                     1111.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTQWDKFVAGNWQQEIDVRNFIQKNYTSYEGDKSFLKGPTEKTQ
                     AVWNKILELLEKEREVGVLDVEVNKVSGIDNYEAGYIDKDNEVILGLQTDAPLKRIVNP
                     FGGWRMVLNSLDAYKYKLNEDIENYFPKYRKTHNDGVFDAYTEETRAARSAGLLTGLPD
                     AYGRGRIIGDYRRVALYGVDFLIMKKREDLKNLKGDMLDELIRKREEVSEQIRALESMK
                     SMALKYGIDMSAPASNAKEAVQFLYLAYLSAVKENNGAAMSLGRTSTFLDIYIERDLKA
                     GILTEEEAQELIDQFIIKLRIVRHLRTPEYDELFAGDPTWVTESIGGIGVNGRPLITKN
                     SFRYLHTLINLGPAPEPNMTVLWSEDLPQSFKDYCSEMSILTDSIQYENDDIMRPIYGD
                     DYAIACCVSAMTVGKQMQFFGARCNMAKALLYSINGGVDEKKGKLVIEGIQPMKDEILD
                     YNKVKESFYKVLEKVAKLYVDTMNVIHYMHDKYAYEAGQMALHDTDVQRLMAFGAAGLS
                     VVADSLSAIKYAEVKPIRNEAGIAVDFEINGDFPKYGNDDDRVDDIAVEVVTKFSSELK
                     KHPLYRNAKHTLSILTITSNVVYGKKTGATPDGRKSGVPFAPGANPMHGRDSMGALASL
                     NSVAKIPYANVCEDGVSNTFSIVPQALGKDEKSRIKNLSAVMDGYFNQGAHHLNVNVFN
                     RETLLDAMDHPEKYPQLTIRVSGYAVMFNRLSREQQLEVISRTFHNSL"
ORIGIN
        1 agttatatat gtgatgtatg cggttacgtt tatgatcctg cagttggaga tgaagataac
       61 ggaataaatc ctggtacttc tttcgaagaa ctgccagaag attgggtttg cccactttgc
      121 ggtgttggta aagaccaatt ttcaccagta gaatagtaaa taaaaaaaat ataaggcgta
      181 acgccttata tttttttgct tattagagtt ttaagatagt cctctaattc tttattcttt
      241 tccttatcca tctccggaac tccttctaat ctatactttt tccctaaagc ttcatatttt
      301 gaaaccccaa gaagatgata tggtagaagt tcaacttttt ctacattatt taatgtattt
      361 attatatcag caagaccttt tatatactcc ctactgtcat ttaagttggg aattataacc
      421 tgtctaatcc acattttatt acctgactgc tgcaccgcct ctaaaaattt cagactctca
      481 ttaatattgt ttctagtcat ttttttatat tcttcagcat ctatatgctt aatatcaaat
      541 aatactaaat ctgtatattt taatatctca gagtatttcc caataccgca gcctgcagta
      601 tctattgtgg tatgaatacc gttttccctg cataatttca gcatttcaat aagaaaatcc
      661 ggctgaagca gcggttcccc tccagaaaag gtaatacctc caccagatga cttaaaatag
      721 gttttatatc ttaatatctt ttttaagaga tcctgtgaag ttatttcttc tccctcattt
      781 gccccccagg tatccgggtt atggcagaat gcacatcgta atgaacatcc ttgaaaaaaa
      841 accactactc tcactccagg gccatctaca agccccatgg tctcaataga atggattttt
      901 cctaacaaat taacaccttc ttatagaaaa aggagaatat ctctattccc cttttaatta
      961 ttttataaac tattgtgaaa ggttctgctt ataacttcaa gctgctgttc tctggataat
     1021 ctgttaaaca taacagcata tcctgaaact ctaattgtaa gctgaggata tttttcaggg
     1081 tgatccatag catccaataa agtctcccta ttaaacacat taacatttaa atggtgagcc
     1141 ccctgattaa agtatccatc cataactgca gataaattct taattctgct cttctcatcc
     1201 ttacctaaag cttgaggcac tatagagaag gtatttgaca ctccatcctc acaaacgttg
     1261 gcataaggta tctttgcaac tgagttaagg gatgctaacg ctcccatact atctcttcca
     1321 tgcattgggt tagctccagg tgcaaaaggt actcctgatt tccttccatc cggtgttgct
     1381 ccggttttct ttccataaac cacattagag gttattgtaa gtatggaaag tgtatgcttt
     1441 gcattcctgt ataaaggatg cttctttaat tcagatgaga atttagtaac cacttctaca
     1501 gcaatatcat ctaccctgtc atcatcgtta ccgtatttag gaaagtctcc attaatctca
     1561 aaatccactg ctattcctgc ttcatttctt ataggcttaa cttctgcata ttttatagca
     1621 cttaatgaat cagctaccac agataagcct gctgcaccaa aggccataag cctctgcaca
     1681 tcagtatcat gaagagccat ctgacctgct tcataggcat atttatcatg catataatgg
     1741 ataacattca tagtatctac atataacttg gctacctttt caagaacctt atagaagctt
     1801 tccttgactt tattataatc cagtatttcg tccttcatag gctgaatacc ttctattaca
     1861 agcttaccct tcttttcatc tacaccgccg ttgattgaat atagaagcgc ctttgccata
     1921 ttacaccttg ctccaaagaa ctgcatctgc tttcctacag tcatggcaga aacgcagcag
     1981 gctatagcat aatcatcacc gtatataggc ctcattatat catcattttc atattgtata
     2041 gaatcagtca atatagacat ttcagaacag taatccttaa agctttgagg aagatcctct
     2101 gaccacagca ctgtcatatt tggttctggt gcaggtccta aattaataag ggtatgaaga
     2161 tacctaaaag agtttttagt tatgagcggt cttccattaa cccctatccc tcctatggat
     2221 tctgtcaccc aggttgggtc tcctgcaaag agttcatcat attctggagt tctcagatgc
     2281 cttacaattc ttaacttaat tataaattga tctataagtt cctgagcttc ttcttcagtc
     2341 aatattcctg cctttaaatc cctttctata taaatatcta agaatgtact cgttcttcca
     2401 agggacatag ctgcaccgtt attttctttt actgcagaca gatatgcaag atataaaaac
     2461 tgtacagctt cttttgcatt ggatgcagga gcggacatat ctattccata ttttaaagcc
     2521 atggacttca tagattccaa agctctaatc tgctcactta cctcttctct ttttcttata
     2581 agctcatcaa gcatatctcc ttttaaattt tttaggtctt cacgcttttt cattataaga
     2641 aaatcaacac cgtaaagtgc tactcttctg taatctccta tgattcttcc tctgccgtaa
     2701 gcatccggta aaccagtgag aagtccggca gatcttgcag ctctggtttc ttcagtatac
     2761 gcatcaaaaa caccgtcatt atgcgttttt ctatatttag gaaaataatt ttcaatatcc
     2821 tcgtttaatt tatatttata agcatccaga gaattaagaa ccattctcca gcctccgaaa
     2881 ggattgacta ttctttttaa aggagcatca gtctgcagtc ccaggataac ttcattatcc
     2941 ttatcaatat aacctgcttc ataattgtct attccggata ctttattaac ctctacatcc
     3001 agaactccta cttctctttc cttttctaaa agctcaagta ttttattcca tacagcctga
     3061 gttttttctg taggcccttt tagaaagctt ttatctcctt cataagaggt ataattcttc
     3121 tgtataaaat ttcttacgtc aatttcctgc tgccaattac ctgcaacaaa cttatcccat
     3181 tgtgtataca ttctattcct ccttagatta acatttaaca atagatgtta ttacataact
     3241 attataactt aatatt
//
