LOCUS       c00023_NZ_LSME..       11644 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_LSME01000003.1 Kluyvera georgiana strain WCH1410 NODE_3, whole
            genome shotgun sequence.
ACCESSION   NZ_LSME01000003
VERSION     NZ_LSME01000003
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 15:34:34
            Original ID  :: NZ_LSME01000003.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 70381
            Orig. end    :: 82025
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11644
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[75381:77025](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6644
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..11644
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11644
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     CDS             1880..2587
                     /locus_tag="ctg23_71"
                     /transl_table=11
                     /translation="VVIGPFINAGAVLVGGLIGALLSQRLPERIRVSMTSIFGLASLGI
                     GLLLVIKCANMPVMVLATLVGALIGEFCHLEKGINNGVSRLQKRLIKPGKSREHETFIQ
                     SYVAIIVLFCASGTGIFGSMHEGMTGDPTILIAKSFLDFFTAMIFACSLGFAVCAISVP
                     MLVIQLTLAFCAFMILPLTTPAMMADFTAVGGLLLLATGLRICGIKMFAVVNMLPALLL
                     AMPISAAWTAFFA"
     PFAM_domain     1886..2560
                     /aSDomain="DUF554"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04474.14"
                     /description="Protein of unknown function (DUF554)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_71_0001"
                     /evalue="1.50E-67"
                     /label="ctg23_71"
                     /locus_tag="ctg23_71"
                     /protein_end="227"
                     /protein_start="2"
                     /score="227.4"
                     /tool="antismash"
                     /translation="IGPFINAGAVLVGGLIGALLSQRLPERIRVSMTSIFGLASLGIGL
                     LLVIKCANMPVMVLATLVGALIGEFCHLEKGINNGVSRLQKRLIKPGKSREHETFIQSY
                     VAIIVLFCASGTGIFGSMHEGMTGDPTILIAKSFLDFFTAMIFACSLGFAVCAISVPML
                     VIQLTLAFCAFMILPLTTPAMMADFTAVGGLLLLATGLRICGIKMFAVVNMLPALLLAM
                     PIS"
     CDS             2931..4199
                     /gene_functions="other (smcogs) SMCOG1077:phage integrase
                     family protein (Score: 70.6; E-value: 3.3e-21)"
                     /gene_kind="other"
                     /locus_tag="ctg23_72"
                     /transl_table=11
                     /translation="MPLSDTAIRNAKPLDKPYKLTDAQGLYLLIKPNGSKLWQLKYRFG
                     GKEKKLAFGAYPVVSLANARKLREEAKATLSAGGDPGEKKRQEKQAKKSGNTFEEVARK
                     WLAKNIRWSENHAAKIQRSLELHVFPLIGKFPVTDLKTADLLIPLRVAEKKGNLETAAR
                     IQQRTTAIMRYAVQESLIASNPANDLTGAIEPPQKNHYPALPLERLQELLEHIEGYPGR
                     LLTRLAVLLNLLIFIRSSELRFARWTEIDLDGAMWTIPAQREPIPDVRYSERGAKMKTP
                     HLVPLSKQAVTVLKQLKLLSGNGELLFPGDHDPRKPMSENTINKALRTMGYDTKVDVCG
                     HGFRTMACSALIESGRWSKDAVERQMNHQERNSVRAAYIHKAEHLDERTQMMQWWSDYL
                     DVCRQKFVPPYRYEQQPSMIRVY"
     PFAM_domain     2937..3191
                     /aSDomain="Arm-DNA-bind_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13356.8"
                     /description="Arm DNA-binding domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_72_0002"
                     /evalue="6.80E-18"
                     /label="ctg23_72"
                     /locus_tag="ctg23_72"
                     /protein_end="87"
                     /protein_start="2"
                     /score="64.8"
                     /tool="antismash"
                     /translation="LSDTAIRNAKPLDKPYKLTDAQGLYLLIKPNGSKLWQLKYRFGGK
                     EKKLAFGAYPVVSLANARKLREEAKATLSAGGDPGEKKRQ"
     PFAM_domain     3549..4076
                     /aSDomain="Phage_integrase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00589.24"
                     /description="Phage integrase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_72_0003"
                     /evalue="9.70E-19"
                     /label="ctg23_72"
                     /locus_tag="ctg23_72"
                     /protein_end="382"
                     /protein_start="206"
                     /score="67.9"
                     /tool="antismash"
                     /translation="ERLQELLEHIEGYPGRLLTRLAVLLNLLIFIRSSELRFARWTEID
                     LDGAMWTIPAQREPIPDVRYSERGAKMKTPHLVPLSKQAVTVLKQLKLLSGNGELLFPG
                     DHDPRKPMSENTINKALRTMGYDTKVDVCGHGFRTMACSALIESGRWSKDAVERQMNHQ
                     ERNSVRAAYIHKA"
     CDS             complement(4479..4787)
                     /locus_tag="ctg23_73"
                     /transl_table=11
                     /translation="MNDMLRQDHPLVLATVLKALNDPTRLRIIQELINDEIGAERHCTS
                     FSGLSEVARATRSHHFKVLREAGLVSLVDRGNLLLARLRRDEIEKAYPGLLNILARG"
     PFAM_domain     complement(4560..4745)
                     /aSDomain="HTH_20"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12840.9"
                     /description="Helix-turn-helix domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_73_0004"
                     /evalue="3.40E-10"
                     /label="ctg23_73"
                     /locus_tag="ctg23_73"
                     /protein_end="76"
                     /protein_start="14"
                     /score="39.9"
                     /tool="antismash"
                     /translation="TVLKALNDPTRLRIIQELINDEIGAERHCTSFSGLSEVARATRSH
                     HFKVLREAGLVSLVDRG"
     CDS             5001..6644
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_74"
                     /sec_met_domain="AfsA (E-value: 7.3e-19, bitscore: 62.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSVYQSQENKLSFDGSIDRRYVHRQAINEVFITDSQQVDSNHFI
                     FSAMLPKSHMYFNDLPELTDGHRCYDAMLLLEVFRQTSIYVTHKYYDVPLNAKFIFNNA
                     EFKILNYPLLEIMQQPLHSVIQVKITNLKYRKKILAGYTLEMTLLINNIACAQRIMGIG
                     WMDDTVWKKLRAKNENLPPLNYNNIKPAQCTSVGRIFPRNVVIGDVQIKDSTLSATLIV
                     DQSYSSIFDHPLDHIPGMFIIEACRQAALLAVNSYKGTPANQLILYNCNMSFQQFCELS
                     STAQCIVDLHEITVTGTLINVPISVLQNGTKNTIGTIILKVVNDAEYEHKEKTDFYWFD
                     FGGVLSPPISSLFDLYYEKTGIPTGQLQAAMKSVADDMNLPTLAPVENAILTELEWGSR
                     LRETMARLFPETDTRRAQLEHFGQQWFAHVTANAAMVKQITDMRNAGYRVGILTNNVVE
                     WRPYWQSMVGLNDVVEHIVDSCDARCRKPDPSFFALAEQVAGVTPEQCVLIDDLVENCL
                     AAEKRGWRTIQFLNNEDCLNKLHTLTYGEE"
     PFAM_domain     5064..5477
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_74_0005"
                     /evalue="2.60E-17"
                     /label="ctg23_74"
                     /locus_tag="ctg23_74"
                     /protein_end="159"
                     /protein_start="21"
                     /score="63.1"
                     /tool="antismash"
                     /translation="YVHRQAINEVFITDSQQVDSNHFIFSAMLPKSHMYFNDLPELTDG
                     HRCYDAMLLLEVFRQTSIYVTHKYYDVPLNAKFIFNNAEFKILNYPLLEIMQQPLHSVI
                     QVKITNLKYRKKILAGYTLEMTLLINNIACAQRI"
     PFAM_domain     5586..5867
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_74_0006"
                     /evalue="1.40E-19"
                     /label="ctg23_74"
                     /locus_tag="ctg23_74"
                     /protein_end="289"
                     /protein_start="195"
                     /score="70.5"
                     /tool="antismash"
                     /translation="VGRIFPRNVVIGDVQIKDSTLSATLIVDQSYSSIFDHPLDHIPGM
                     FIIEACRQAALLAVNSYKGTPANQLILYNCNMSFQQFCELSSTAQCIVD"
     PFAM_domain     6189..6581
                     /aSDomain="HAD_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13419.8"
                     /description="Haloacid dehalogenase-like hydrolase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_74_0007"
                     /evalue="5.50E-08"
                     /label="ctg23_74"
                     /locus_tag="ctg23_74"
                     /protein_end="527"
                     /protein_start="396"
                     /score="33.2"
                     /tool="antismash"
                     /translation="GSRLRETMARLFPETDTRRAQLEHFGQQWFAHVTANAAMVKQITD
                     MRNAGYRVGILTNNVVEWRPYWQSMVGLNDVVEHIVDSCDARCRKPDPSFFALAEQVAG
                     VTPEQCVLIDDLVENCLAAEKRGWRTI"
     aSDomain        6393..6584
                     /aSDomain="TIGR01509"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IA-v3: HAD hydrolase, family IA,
                     variant 3"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg23_74_0001"
                     /evalue="2.30E-11"
                     /identifier="TIGR01509"
                     /label="ctg23_74"
                     /locus_tag="ctg23_74"
                     /protein_end="528"
                     /protein_start="464"
                     /score="42.1"
                     /tool="antismash"
                     /translation="SMVGLNDVVEHIVDSCDARCRKPDPSFFALAEQVAGVTPEQCVLI
                     DDLVENCLAAEKRGWRTIQ"
     CDS             6647..7843
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 262.2; E-value: 1.2e-79)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg23_75"
                     /sec_met_domain="p450 (E-value: 8.8e-18, bitscore: 58.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNRTYRITDIPHYDPQMGYEIIKAATYQEIGIIRLLSGHEAFHV
                     INYQDVKKILMDKTCLRSPSNETGGPSVLPTLTPKELLLNLDFPDHTRVKQFVARDYSR
                     SGLKWLEPVITRLVLGYVEKMALKKQPDLFTDLLDHVAADTNCTLLGLPLEDKHYFRKL
                     SHIVQVANPDDVEDLVHQFELLYEYVMEHVQNKRHHGKDGLIARFVQNRENCQPPLNDA
                     ELTAILLGSVLGGDQNTLTVMTKIIYGLLYMPGIWRQLHDEPTLVPAMVEEMLRLTNLG
                     STSTFPRIATQDIQLDTGVIPTGAAIHADVFLANRDPAVYYDPLLINPYRENNQHLQFG
                     YGMHHCMGQELARLEINTVITLLTRQYPQMRLRHGNLNDLPWCEGIVLRRPDALPVII"
     PFAM_domain     7187..7744
                     /aSDomain="p450"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00067.24"
                     /description="Cytochrome P450"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_75_0008"
                     /evalue="5.10E-16"
                     /label="ctg23_75"
                     /locus_tag="ctg23_75"
                     /protein_end="366"
                     /protein_start="180"
                     /score="58.6"
                     /tool="antismash"
                     /translation="QFELLYEYVMEHVQNKRHHGKDGLIARFVQNRENCQPPLNDAELT
                     AILLGSVLGGDQNTLTVMTKIIYGLLYMPGIWRQLHDEPTLVPAMVEEMLRLTNLGSTS
                     TFPRIATQDIQLDTGVIPTGAAIHADVFLANRDPAVYYDPLLINPYRENNQHLQFGYGM
                     HHCMGQELARLEINTVITLLTRQ"
     CDS             7859..9382
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 280.7; E-value:
                     2.7e-85)"
                     /gene_kind="transport"
                     /locus_tag="ctg23_76"
                     /transl_table=11
                     /translation="MSQPNSRLLLFAILLAVFVVPGSISGTAIALPFISADMNASLSAL
                     QWVVNAFNLTFACFTLFWGTLADACGRKKAFLSGTTIYAVASVMSALSPGALYLDISRA
                     LAGIGGAAIFSCGSAILIAQFEGSQRTKAFALFGTTAGIGITFGPTISGFILDFINWRA
                     IFAVHAITLAVVLILAKSIPGDTATNKRFRFDIAGTVLFITALLLFMLAMVQGAQWGWT
                     DIRTLSLLILAAVITVGFYLYENRIDNPMLNFNLLKNRTFVGLILIPVVASVIFVTLLT
                     YYPSYLTGVMQLTPSKAGLMMIALTLPVLFCPVIAGRLVSGGASARMILFISLAAFIAG
                     GITLILFSQPDRELWILTIPLLLIGTGMGLSAGLVDGLALSCVDPLQAGMAAGLLNTLR
                     LGSEALAVALYASLLTSHLTGSLPGLFMQFSLPANQLTEWINAVASGNLTAPLKHAPGG
                     NLLALIIAQYHAAFIYVVDILNIITVALSLWILRLLMNKRAMTRQQVKE"
     PFAM_domain     7895..9058
                     /aSDomain="MFS_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07690.18"
                     /description="Major Facilitator Superfamily"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_76_0009"
                     /evalue="8.10E-44"
                     /label="ctg23_76"
                     /locus_tag="ctg23_76"
                     /protein_end="400"
                     /protein_start="12"
                     /score="150.1"
                     /tool="antismash"
                     /translation="ILLAVFVVPGSISGTAIALPFISADMNASLSALQWVVNAFNLTFA
                     CFTLFWGTLADACGRKKAFLSGTTIYAVASVMSALSPGALYLDISRALAGIGGAAIFSC
                     GSAILIAQFEGSQRTKAFALFGTTAGIGITFGPTISGFILDFINWRAIFAVHAITLAVV
                     LILAKSIPGDTATNKRFRFDIAGTVLFITALLLFMLAMVQGAQWGWTDIRTLSLLILAA
                     VITVGFYLYENRIDNPMLNFNLLKNRTFVGLILIPVVASVIFVTLLTYYPSYLTGVMQL
                     TPSKAGLMMIALTLPVLFCPVIAGRLVSGGASARMILFISLAAFIAGGITLILFSQPDR
                     ELWILTIPLLLIGTGMGLSAGLVDGLALSCVDPLQAGMAAGLLNTLRL"
     CDS             complement(9520..10404)
                     /locus_tag="ctg23_77"
                     /transl_table=11
                     /translation="MGMLMQEMSRLARQAGGSNKTVHDRMALAQRFCERLSVVQNIQIR
                     QVAQLKTRHIEDYIRERLAQGIARRSLQNEMAAIRCLLVQAGRNKLATSERISNRALGL
                     AGAPRTGTHRAIPAVYYRQVLDVARTKDAGLTAALELSRLMGLRSQEAVQSAQSLKTWR
                     QAIERGEPRLTVVFGTKGGRSRETRILDTGAVRKALDNAIAVAESRNGRLIDKPDLRTA
                     MKYWHSQASRIGLTGAYSPHSLRYAWAQDAIRHYLAQGFSEKEALAQTAMDLGHGDGRG
                     QYVRLVYGREIVL"
     PFAM_domain     complement(9652..10098)
                     /aSDomain="Integrase_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12835.9"
                     /description="Integrase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_77_0011"
                     /evalue="1.20E-48"
                     /label="ctg23_77"
                     /locus_tag="ctg23_77"
                     /protein_end="251"
                     /protein_start="102"
                     /score="165.2"
                     /tool="antismash"
                     /translation="GLAGAPRTGTHRAIPAVYYRQVLDVARTKDAGLTAALELSRLMGL
                     RSQEAVQSAQSLKTWRQAIERGEPRLTVVFGTKGGRSRETRILDTGAVRKALDNAIAVA
                     ESRNGRLIDKPDLRTAMKYWHSQASRIGLTGAYSPHSLRYAWAQD"
     PFAM_domain     complement(10129..10404)
                     /aSDomain="Phage_int_SAM_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12834.9"
                     /description="Phage integrase, N-terminal"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_77_0010"
                     /evalue="5.10E-33"
                     /label="ctg23_77"
                     /locus_tag="ctg23_77"
                     /protein_end="92"
                     /protein_start="0"
                     /score="113.6"
                     /tool="antismash"
                     /translation="MGMLMQEMSRLARQAGGSNKTVHDRMALAQRFCERLSVVQNIQIR
                     QVAQLKTRHIEDYIRERLAQGIARRSLQNEMAAIRCLLVQAGRNKLA"
ORIGIN
        1 cttgcacggg tcaacgaagt actgttcctg cgcatagccc tcaaaaccgt gccagcgcgc
       61 gcccggcgtg aagctaaaga agcgacgttc gctggcaatc gttttggttg ggtgatcctg
      121 ccacgggcgg ccatcaacca tcggcggaat aaacggctgg atcatcttgc agttcgccac
      181 aatcgcctta cgcgcttcaa tacccagctc aacgcattct gcccacagac ggcgaccact
      241 ctccccttcg tggattttgg cgttgatatc caacgccgca aacagcgggt aaaacgggct
      301 ggttgaggcg tgcagcataa aggcgttgtt cagccgtttg tgcgggcaaa agcgcgcctg
      361 gccgcgaata tggttatctt ttttgtggat ctgcgaggtc tgcgagaagc ccgcctgctg
      421 tttgtgcacc gactgggtaa cgaaaatgcc cggatcgttt tcgttgagat ccagcagcag
      481 cggcgaggtg tcggacatca tatcaataaa ctgctcgtag cctacccacg cggagtcaaa
      541 cagaatgtag tcgcaaaggt ggccgatttt atccaccacc tgacgcgcat tgtagatagt
      601 gccgtcatag gtgcccagct gaatgatcgc caggcggaac ggacgcgtat catcggcttt
      661 ctccggtgcg gcttcacgaa tctgctcgcg caggtagcgt tcgtcaaagc agtgctcatc
      721 aatgccaccg atgaaaccga acgggttacg cgccgcttcc agatagaccg gcgtcgcgcc
      781 agcctgaatc agcgcgccgt ggtggttcga tttgtggttg ttgcggtcga acagcaccag
      841 atcgccgcgg gtcagcagcg cattggtcac caccttattg gcggcggaag tgccgttcag
      901 cacaaagtag gttttatcgg cgtggaacac cttcgcggca aacttctgcg cgtgcttggc
      961 tgaaccttcg tgaatcagca ggtcgcccag cttcacgtcg gcattgcaca tgtcggcacg
     1021 aaacacgttc tcgccgaaga agtcataaaa ctgacggccc gccggatgct ttttaaagaa
     1081 cgcgccgtgc tgatggcccg ggcaggcaaa ggtgctgtta cccatttcaa cgtactgagt
     1141 cagggtatta aaaaacggcg gcagcagctc ttcttcatag cgacaggcgg cggtttccag
     1201 ctccagccac tcctgttctt taccgctgat gaccgcatct acgccgtttg gggcgctgat
     1261 atcatcgctc gtcagcataa ataccggcag cttaaatccg ctgcgtttga gtaacgcaag
     1321 aatgccgctg tggctatcgg ccacggtaat gacgactgct gccacatcgg taaagtcggt
     1381 actatccagc gccaccacga cacgatgggt ggaaaggcgt gagaccagtt cacgactggc
     1441 agcaatgttc attgatttca taagcgcaac gacccactca gggagagtaa aagtcctggg
     1501 cagagcatct ggctctgccc cacgaagcgt ctgggtgcaa ctgggttaca gctccgaccg
     1561 ccagacatca gtaaaagcag acatggtcta atcttactgt tgtcctgcag tgagcgtacg
     1621 ttggcgtcac cgcatggtga gcaatgagaa aggggcagac cttcgcgagc aggcgaagcg
     1681 gcattggcga tgtaatggca aagatgtcat tgcaggcggc cccaattcag gaaaggagaa
     1741 atttcgcgca atcatggcac cattcaccag agggtgcaag acgaaatttt tatgcaccaa
     1801 taccacagag aaacaagcac tgaaccgatt aatcagtcca taataatgca ggacttatat
     1861 ataagtaaca ggagttaacg tggtaattgg cccatttatt aatgccgggg cggtgctcgt
     1921 cggtggtttg atcggcgcgt tgttaagcca gcgtcttccg gagcgtattc gcgtctccat
     1981 gacctcgatc ttcggcctcg cctcgttggg tattggcctg ctgctggtga tcaagtgcgc
     2041 caacatgccg gtgatggtgc tggccacact ggtgggagcg ctgatcggtg agttttgcca
     2101 tctggaaaaa gggatcaaca acggcgtctc ccgactgcaa aaacggctga ttaagcccgg
     2161 caaaagccgg gaacatgaga catttattca aagctacgtg gcgatcatcg tgctattttg
     2221 cgcgagcgga accgggattt ttggttcaat gcacgaagga atgaccggcg atccgaccat
     2281 actcatcgcc aagtcgtttc tcgatttctt taccgccatg attttcgcct gctcactcgg
     2341 ctttgccgtc tgcgccatta gcgtgccgat gctggtcatc caactgacgc tggccttctg
     2401 cgcctttatg atcctgccgc tgaccacccc tgcaatgatg gctgatttca ccgccgtcgg
     2461 cggcctgtta ctgttggcaa ccggcctgcg tatctgcgga atcaagatgt tcgccgtggt
     2521 gaatatgctg ccagcgctgc tgctggcgat gcctatttcc gctgcctgga ccgccttttt
     2581 tgcctgacgt cgcgtgcaaa atacgcaagg tgatgataga ttgtgcaact cgccgcaaaa
     2641 tttataattt tcgttgacga ggcgaggcga ttacggttta atgcgccccg ttgcccggat
     2701 agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc cttggttcga ttccgagtcc
     2761 gggcaccata tttttgtctt gggatgtctc agtaagtccg aggacgtata aaaatcagta
     2821 agttaaagag acccgatggt atcattgaat accaacgggt cttttgacat ccatggtttt
     2881 tgggggctta attgggggcc tgtcggttcg ataaactatg gagccccacc atgccactct
     2941 ccgataccgc tatccgcaac gcaaaacctc tcgataaacc ttacaagctt accgatgcgc
     3001 aaggtctgta cctgctgatt aagcctaatg gctctaaact ctggcaactc aagtaccgtt
     3061 ttggcggtaa agagaaaaag ctggcgtttg gtgcttaccc tgtcgtttcg ctggcgaatg
     3121 cccgtaagct acgtgaagaa gccaaagcta ccctcagcgc tgggggcgat ccaggtgaga
     3181 aaaaacgaca ggagaaacag gcgaaaaaaa gcggcaatac ttttgaagaa gtcgcccgaa
     3241 aatggctggc aaaaaatatc cgctggtcag aaaaccacgc cgcaaaaatc cagcgctcgc
     3301 tggagctgca tgtattcccg ctcataggca aatttcctgt caccgatttg aaaactgccg
     3361 atctgctgat cccgctgcgg gtggctgaga aaaaggggaa tctggaaacc gccgcgcgga
     3421 tacagcaacg caccacagcc attatgcgct atgcggtgca ggaaagcctg attgccagca
     3481 acccggcgaa tgatcttacc ggagccatag agcctccgca gaaaaaccac taccccgccc
     3541 tgccactgga aagattacag gaactgctgg agcatattga aggctatccc ggcagactac
     3601 tgacacggct ggcggtactg ctgaatctgc tgatcttcat ccgctccagc gaactgcgct
     3661 ttgcccgttg gacagagatc gatctggatg gtgctatgtg gactattccc gcccagcgtg
     3721 agcccattcc ggacgtgcgc tattcagagc gtggcgcaaa aatgaaaaca ccgcatctgg
     3781 tgccgctgtc aaaacaggcg gttacggtac tgaaacagct aaaattactg tccggcaacg
     3841 gtgaattgct gtttcccggc gaccacgatc cgcgtaagcc gatgagcgaa aacaccatca
     3901 acaaagcact gcgtacaatg ggttatgaca cgaaggtgga tgtttgcggc cacggctttc
     3961 gcacaatggc ctgtagtgcg ctgatcgagt ccggacgctg gtcgaaagat gccgtggaac
     4021 ggcagatgaa tcatcaggag cgtaacagcg tgcgggctgc ctatatccac aaggctgagc
     4081 acctcgacga gcgcacgcaa atgatgcagt ggtggtcaga ctatctagac gtctgtcggc
     4141 agaaatttgt tccgccgtat cgctatgagc agcaacccag catgatcaga gtttattaac
     4201 gtacagtact aaaaagcagc tctcccgatg ctaagcggaa ctgcttttta ttgctccagt
     4261 attgagacct gcccaccagc atcagtttca cgtagtcgaa aaatctcaac agcaggtcaa
     4321 gccttttacc ctttagggta agacttttac ccttaaacat cccttaccat ttagcccttt
     4381 agccctttac ctggaatctg acgctttcag taatagcgca caatattcag cgtcaggcac
     4441 cggacactac ggcccaattg gtgatgacag atatgacttt agccacgggc aagaatgttg
     4501 agtaaaccag gataagcttt ttcgatttca tcccgcctca gccgggctaa cagcaaattt
     4561 ccccggtcaa ccagagaaac aagacccgct tcgcgcaaga ctttaaaatg atgcgatctt
     4621 gtcgcgcggg ctacttcaga cagaccgctg aacgatgtac agtgcctttc tgctccgatc
     4681 tcatcgttga ttagctcctg gatgattctt aaccgtgttg ggtcatttaa agccttcagc
     4741 acagtagcca aaaccagtgg atgatcctgt cttagcatgt cattcattcc atcactccca
     4801 gattaaccca ttgatactat agtaaatatc gcctacacaa caaagataag ttgtaataaa
     4861 ttgacattta attacttttc gcacatagtg cgttcgatga acatcgaacg ttcattttta
     4921 atgatacgtt cgatgttcat cgaatatacc attacagcca gaatgtgtca ctcactacac
     4981 atgcaatgga gaatgtccat atggattctg tatatcagtc tcaggaaaat aagttatctt
     5041 ttgatggctc gattgataga agatatgttc accggcaggc tatcaacgag gttttcatca
     5101 ctgactcaca acaagtggac agtaatcatt ttatattttc cgccatgttg ccaaaatcgc
     5161 acatgtattt taatgatctc cccgaactca ctgatggtca ccgatgttat gacgcaatgt
     5221 tactgttaga agtcttccgg caaacatcta tttatgtgac acacaaatac tatgatgtcc
     5281 ccctcaatgc aaaatttatc tttaataatg ccgaatttaa aatccttaat tatcctcttt
     5341 tggagataat gcagcagcca ttacattcgg taattcaggt caaaatcacc aacctaaagt
     5401 atcgcaaaaa gatcctggct gggtatacct tagaaatgac actcctgatt aacaatatcg
     5461 cctgtgctca gagaataatg gggattggct ggatggatga taccgtctgg aaaaaattaa
     5521 gagcgaaaaa tgagaatctc ccccccttaa attataacaa tattaaacca gcgcaatgca
     5581 catctgttgg ccggatcttc ccgcgcaatg tcgttattgg tgatgtgcag ataaaagata
     5641 gcacgctctc agccacactt attgtcgatc aatcctactc gtctattttt gaccacccat
     5701 tagatcatat tcctggcatg tttattatcg aggcctgccg acaagcagct ctgttggcag
     5761 tcaattcata taaaggtaca ccagcaaacc aactgatact ctataattgc aatatgtctt
     5821 tccagcaatt ctgtgaatta agcagcacag ctcaatgtat cgttgacctg catgaaatca
     5881 cagtcactgg cactttaatt aatgtgccaa tttctgtttt acaaaacggc actaaaaata
     5941 ccattgggac tatcatactt aaagttgtaa atgatgctga atacgaacat aaagaaaaaa
     6001 cggatttcta ctggttcgac tttggtggcg tattatctcc tcctatttct tcattattcg
     6061 acctttatta tgaaaaaacc ggaattccga ctggccagct acaggccgca atgaaatcgg
     6121 tcgctgatga tatgaacctg cccacgctgg ccccggttga aaatgccata ttaaccgagc
     6181 tggaatgggg atcgcgtctc agggaaacaa tggcacgtct ttttcctgaa acagataccc
     6241 gccgggcaca gttagaacat ttcggccagc aatggtttgc tcacgttacc gccaacgctg
     6301 caatggtaaa gcaaattaca gatatgcgga acgctggtta tcgggtcggt atcctcacga
     6361 ataatgtggt cgagtggcgg ccctactggc agtccatggt tggtctcaac gatgtcgtcg
     6421 aacatattgt cgattcctgt gatgcccgat gtcggaaacc agacccttcg ttttttgctc
     6481 ttgcggaaca ggttgccgga gttacgcctg agcagtgtgt actcattgat gatttggtcg
     6541 aaaactgcct cgctgctgaa aagcgtggct ggcggacgat ccagttcctc aacaatgagg
     6601 attgtttaaa caaactgcac accttaactt atggagagga ataataatgg acaacagaac
     6661 atatcgtata acggatatac ctcattacga tccccaaatg ggatatgaaa ttataaaggc
     6721 cgccacttat caggagatcg gaattattcg tctgctgagt gggcacgagg cgtttcatgt
     6781 catcaattat caggatgtca aaaaaatcct gatggataag acctgcctcc gcagcccctc
     6841 taacgaaaca ggtgggccca gtgtgctgcc aacgctaacg ccaaaggaac tgttgctaaa
     6901 tctggatttt cccgaccaca ctcgcgtaaa acaatttgtc gccagagact acagcagaag
     6961 cggattgaaa tggctggaac ccgttattac gcgcctggta ctgggatatg tagaaaagat
     7021 ggcgctaaaa aaacaacccg atctgtttac tgatctgctg gatcacgttg ctgctgatac
     7081 aaactgtacc ttgctgggac ttccccttga agataaacac tattttcgca agctgagtca
     7141 tattgtacag gtagcgaatc ctgatgatgt tgaagatctg gttcatcaat ttgaattact
     7201 ttacgaatat gtcatggagc atgttcagaa taaaagacat catggcaaag acggcctgat
     7261 tgccagattt gtgcaaaaca gggaaaactg ccagccacca cttaatgatg cagaattaac
     7321 agctatcctg ctgggttcgg tgctgggagg tgatcaaaac actctgacgg ttatgaccaa
     7381 aatcatctat ggtctgcttt acatgcctgg aatatggaga cagttacacg acgaaccgac
     7441 actcgttcct gcgatggttg aagaaatgct gcggctaacc aacctgggaa gcacatcgac
     7501 ttttccccgg attgccacac aggatatcca actggataca ggcgttatcc cgacaggcgc
     7561 agcgattcac gccgatgtat ttctggcaaa ccgtgacccc gcagtgtatt acgaccctct
     7621 gttaataaat ccttatcgtg aaaataacca gcacctacag tttggttacg gcatgcatca
     7681 ctgcatgggg caggagcttg cccgcctgga aatcaacaca gtcattaccc tgctgactcg
     7741 tcagtatccg cagatgagac tacgccatgg caacctaaac gacctgccat ggtgtgaagg
     7801 tatcgtgcta cgccgtcctg acgcactgcc cgttattatt tgatcatctg gagccattat
     7861 gtctcagcca aatagcaggt tacttttatt tgccatttta ctggcggtat ttgttgtacc
     7921 tggttcaatt tccggaacgg caattgcgtt gccattcatt agcgcagaca tgaacgccag
     7981 cctgtcagcg ttacagtggg tcgtcaacgc ctttaactta acgtttgcct gctttacctt
     8041 attctgggga acgcttgctg acgcctgcgg gcggaagaaa gcctttttat ccggaaccac
     8101 aatttatgcc gtagcttcag tcatgtcggc actctctccc ggcgctttgt atcttgatat
     8161 ttcccgtgct ctggccggga tcggaggtgc cgctattttt tcatgcggca gcgctattct
     8221 gatcgcccag ttcgaaggca gtcaacggac taaggctttc gccctctttg gcaccactgc
     8281 cgggatcggt attacttttg gcccgacaat ttcaggtttt attcttgatt ttatcaactg
     8341 gcgcgccatt tttgctgttc atgccatcac tctggcggtt gtgttaattc tggctaaatc
     8401 tattcccgga gataccgcga cgaataaacg gtttcgtttt gatattgccg gaaccgtact
     8461 ctttatcacc gccctcctgc tgtttatgct ggcgatggta cagggggctc agtggggctg
     8521 gacagatatc agaacgctgt cattattgat acttgccgcc gtgataactg ttggttttta
     8581 tctctatgaa aaccgtattg ataacccaat gctgaatttt aacctgttga agaatcgtac
     8641 cttcgtcgga ctgatcctga tcccggtggt tgccagcgtc atcttcgtga cactgttgac
     8701 gtattatccc agctatctga caggagtgat gcagctcaca ccgtcaaaag ccggtctcat
     8761 gatgatcgca ctcactctgc ccgttctttt ctgcccggtg attgcaggca ggctggtctc
     8821 aggtggggca tcagcccgta tgattctgtt tatcagcctg gctgcattta tcgctggcgg
     8881 tataacttta atccttttca gccagccaga ccgtgaacta tggatattga ccataccatt
     8941 attattaatt ggtacgggta tgggattatc cgccgggctg gtcgatggcc tggcattaag
     9001 ttgcgtggat ccgttacagg caggcatggc tgccggattg cttaatacgc tcagactggg
     9061 tagtgaagcg ctggcagttg cattatatgc ttcattactg acctctcatc tgacagggtc
     9121 tttgcctggt ctttttatgc agttcagtct cccggcgaat caattgacag agtggattaa
     9181 cgctgtcgcc tcaggcaatc tcaccgcccc gctgaaacac gcacctggcg gcaatctgtt
     9241 agcattgata attgcccagt atcatgctgc gtttatttac gttgtagata ttctaaatat
     9301 catcaccgtt gctctgagcc tttggatcct cagattactg atgaataagc gggccatgac
     9361 cagacagcag gttaaagaat agacgtttct ggctgagtat tattcaattc aagcgaatca
     9421 tactcagcct ttattgaatg gagttatacc aggattctgt gcgctttccc ccgcagatca
     9481 taacagtccg gatatctttc ctgcatcata ccgaaaaagt tatagtacta tctcccgacc
     9541 ataaaccaga cggacatatt gcccacgccc atcaccatgc ccaagatcca ttgccgtctg
     9601 tgccagcgct tccttctcgc tgaatccctg cgccagataa tggcggatcg catcctgtgc
     9661 ccaggcatag cgcagggaat gcggggaata tgccccggtt aacccaattc gcgacgcctg
     9721 gctgtgccag tatttcatgg ccgttctcaa gtcgggttta tcaatcagtc tgccgtttcg
     9781 gctttccgcc accgcaatgg cgttatccaa cgcctttctg actgcgccag tatcgaggat
     9841 cctggtttcc cgggatcgtc cgcctttggt gccaaacacc actgtaagcc gtggctctcc
     9901 acgttcaatg gcctgccgcc aggttttcag cgactgtgcg ctttgtactg cttcctgtga
     9961 gcgtagcccc atcagccgcg acagttccag cgccgccgtc agtccggcat ctttcgtcct
    10021 ggcaacgtcc agcacctgcc ggtaatacac cgctggaata gctctatgtg ttcctgtacg
    10081 aggtgcgcca gccagcccca gcgcccggtt gcttatccgt tcactggtcg ccagtttatt
    10141 gcgtccggcc tgcaccagca gacagcgtat cgccgccatt tcattttgca gggaacgccg
    10201 ggcaatcccc tgcgccagcc gctcccggat ataatcttct atatgccggg ttttaagctg
    10261 cgccacctga cggatctgga tattctgcac caccgacaga cgttcacaga accgctgcgc
    10321 tagcgccata cggtcatgga cagttttatt gctgccgccc gcctgccgcg ccagtcggct
    10381 catctcctgc atcagcattc ccataacaaa ttcctctttt catgattgcc acaaaaccca
    10441 catcccatac aactttcccc ggcgcatagg ccgatagccc tgattgattt gtcgggctgc
    10501 gaacgatacc tgagcgccgg ggcggtcgct gttgaggtgt tgtggggtat cggttgtgct
    10561 ctctgagcag ccgcctgctt tcgcaggtca tcccccggat cgtcctgatc cccctccggc
    10621 atcggttcat ggtctcctgt gggttaaaat ggctgaaaag ggtgtgtcag aatcctgcta
    10681 tgcgctggcg cagtggagtt gactggtgca gtgacgtgga tttcgtgcgt cactttcccg
    10741 cgacttcgcg gttaaagtga cgcaccgggc tggcgcagtg ttcaagatta cggttttggt
    10801 gctggtttac ggtcacgcga cagcaaatgc ctgtcggcaa atgcaggccg cagcccgtaa
    10861 ggtaaagagt gctgattgtc agcagatgta gattggccgg attacttcga catggaagaa
    10921 cgttcaccag caggctggtg cagttaaaag gataagtgtg aaatttgacc tctgtcgcag
    10981 tcaactgcga gccggtgttt gcggaggttt gcaccggtag gggctgccat cctgagttcc
    11041 tcaggcgctt ctttcaggcc atgaagcatg ggcgctgtcg acgacagcat tgtgcaatac
    11101 ggagcttaca tccggtcagt gaacgtgaaa agaggaaact cttttttgct aaaaagagtt
    11161 gtttgtagat tttttcacga ttttgtgaat gtaaacttga tactgtattc gtgatttgcc
    11221 ttcggctatc gattcttacg gcctttaagc ctgatttttg taagcaaaca tcatttctac
    11281 acctcaaaac acacgactgg tagcccctct gaatcaccag ttcatcacac tgtatgtcgg
    11341 tgcgaaaaaa ttcaccatcc tgcttaacga taacgtcctg catgcctaac aggtgctgat
    11401 gccaaattgg gttatcagat agttcttctg tgttcagcag acttaatcac agcgatgatc
    11461 tgatactcag tgaaacgaac tttatacata gcgctctcct tcattggcaa attaagtatg
    11521 ccggaagatc tctaaatgtg aatgacacga ttatgcggga tacttacaat aacaacaggt
    11581 tcgactgaag ggattaaaaa tggtgagttt ctaagaaccc taggagacat ggtaagaatt
    11641 gata
//
