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Overview
1.1
1.2
1.3

### Identified secondary metabolite regions using strictness 'relaxed'

NZ_AP022254.1

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | hserlactone | 450,942 | 471,571 |
Region 1.2 | NRPS | 1,735,732 | 1,785,917 | amonabactin P 750 | NRP | 100%
Region 1.3 | RiPP-like | 3,155,608 | 3,166,450 |

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | hserlactone | 450,942 | 471,571 |
Region 1.2 | NRPS | 1,735,732 | 1,785,917 | amonabactin P 750 | NRP | 100%
Region 1.3 | RiPP-like | 3,155,608 | 3,166,450 |

No secondary metabolite regions were found in these records:
  NZ_AP022255.1

Compact view
NZ_AP022254.1 - Region 1 - hserlactone
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 450,942 - 471,571 nt. (total: 20,630 nt) Show pHMM detection rules used
hserlactone: Autoind_synth

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
MIBiG comparison
KnownClusterBlast
Pfam domains
TIGRFAM domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | hserlactone | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001774.1 |  | 0.14 | Other | spicamycin | Streptomyces fimbriatus
BGC0001480.1 |  | 0.09 | Other | tundrenone | Methylobacter tundripaludum 21/22
BGC0001440.1 |  | 0.09 | Other | cystargolide A, cystargolide B | Kitasatospora cystarginea
BGC0000961.1 |  | 0.06 | NRP, Polyketide | bactobolin | Burkholderia thailandensis E264
BGC0000376.1 |  | 0.06 | NRP | JBIR-34, JBIR-35 | Streptomyces sp. Sp080513GE-23
BGC0001106.1 |  | 0.05 | NRP, Polyketide | oxalomycin B | Streptomyces albus
BGC0000379.1 |  | 0.05 | NRP | glycinocin A | Streptomyces viridochromogenes
BGC0001186.1 |  | 0.05 | Polyketide | misakinolide A | Candidatus Entotheonella serta
BGC0000963.1 |  | 0.04 | NRP, Polyketide, Saccharide | bleomycin | Streptomyces verticillus
BGC0001048.1 |  | 0.04 | NRP, Polyketide, Saccharide | tallysomycin A | Streptoalloteichus hindustanus

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001774.1 |  | 0.44 | Other | spicamycin | Streptomyces fimbriatus
BGC0001480.1 |  | 0.34 | Other | tundrenone | Methylobacter tundripaludum 21/22
BGC0001440.1 |  | 0.32 | Other | cystargolide A, cystargolide B | Kitasatospora cystarginea
BGC0000961.1 |  | 0.26 | NRP, Polyketide | bactobolin | Burkholderia thailandensis E264
BGC0000376.1 |  | 0.25 | NRP | JBIR-34, JBIR-35 | Streptomyces sp. Sp080513GE-23
BGC0001106.1 |  | 0.23 | NRP, Polyketide | oxalomycin B | Streptomyces albus
BGC0000379.1 |  | 0.22 | NRP | glycinocin A | Streptomyces viridochromogenes
BGC0001186.1 |  | 0.22 | Polyketide | misakinolide A | Candidatus Entotheonella serta
BGC0000963.1 |  | 0.18 | NRP, Polyketide, Saccharide | bleomycin | Streptomyces verticillus
BGC0001048.1 |  | 0.18 | NRP, Polyketide, Saccharide | tallysomycin A | Streptoalloteichus hindustanus

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names
NZ_AP022254.1 - Region 2 - NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1,735,732 - 1,785,917 nt. (total: 50,186 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
Pfam domains
TIGRFAM domains
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | NRPS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001502.1 |  | 0.75 | NRP | amonabactin P 750 | Aeromonas hydrophila subsp. hydrophila ATCC 7966
BGC0000426.1 |  | 0.37 | NRP | sevadicin | Paenibacillus larvae
BGC0000389.1 |  | 0.33 | NRP | massetolide A | Pseudomonas fluorescens SS101
BGC0000465.1 |  | 0.33 | NRP | xenortide A, xenortide B, xenortide C, xenortide D | Xenorhabdus nematophila ATCC 19061
BGC0000457.1 |  | 0.31 | NRP | vicibactin | Rhizobium etli CFN 42
BGC0000351.1 |  | 0.28 | NRP | exochelin | Mycolicibacterium smegmatis
BGC0001050.1 |  | 0.27 | NRP, Polyketide | thalassospiramide A | Tistrella bauzanensis
BGC0001479.1 |  | 0.27 | NRP | anabaenopeptin NZ857, nostamide A | Nostoc punctiforme PCC 73102
BGC0001671.1 |  | 0.27 | NRP | monobactam | Agrobacterium tumefaciens
BGC0001434.1 |  | 0.27 | NRP | nematophin | Xenorhabdus budapestensis

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001502.1 |  | 0.91 | NRP | amonabactin P 750 | Aeromonas hydrophila subsp. hydrophila ATCC 7966
BGC0000426.1 |  | 0.70 | NRP | sevadicin | Paenibacillus larvae
BGC0000389.1 |  | 0.67 | NRP | massetolide A | Pseudomonas fluorescens SS101
BGC0000465.1 |  | 0.66 | NRP | xenortide A, xenortide B, xenortide C, xenortide D | Xenorhabdus nematophila ATCC 19061
BGC0000457.1 |  | 0.65 | NRP | vicibactin | Rhizobium etli CFN 42
BGC0000351.1 |  | 0.62 | NRP | exochelin | Mycolicibacterium smegmatis
BGC0001050.1 |  | 0.61 | NRP, Polyketide | thalassospiramide A | Tistrella bauzanensis
BGC0001479.1 |  | 0.61 | NRP | anabaenopeptin NZ857, nostamide A | Nostoc punctiforme PCC 73102
BGC0001671.1 |  | 0.61 | NRP | monobactam | Agrobacterium tumefaciens
BGC0001434.1 |  | 0.61 | NRP | nematophin | Xenorhabdus budapestensis

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Module view
Shows module structures for each candidate in NRPS- and PKS regions. Genes are shown in predicted order, and are only present when containing at least one complete module. A domain glossary is available here.
Legend
Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 2, location 1735731 - 1785917:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (X) + (D-X)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_1603: dhb
  Search NORINE for peptide: strict or relaxed
  AMP-binding (36..446): dhb
  NRPSPredictor2: dhb
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic
    Large clusters prediction:
      dhb, sal
    Small clusters prediction:
      dhb, sal
    Single AA prediction:
      dhb
  Stachelhaus prediction details:
    Stachelhaus sequence:
      PLPsQGVVNK
    Nearest Stachelhaus code:
      dhb
    Stachelhaus code match:
      90% (moderate)
ctg1_1605: X
  Search NORINE for peptide: strict or relaxed
  AMP-binding (456..859): X
  NRPSPredictor2: asp, asn, glu, gln, aad
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      asp, asn, glu, gln, aad
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAeDIGtVvK
    Nearest Stachelhaus code:
      glu
    Stachelhaus code match:
      70% (weak)
ctg1_1607: X
  Search NORINE for peptide: strict or relaxed
  AMP-binding (541..927): X
  NRPSPredictor2: (unknown)
  SVM prediction details:
    NOTE: outside applicability domain
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      N/A
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAmigGMVtK
    Nearest Stachelhaus code:
      leu
    Stachelhaus code match:
      60% (weak)
ctg1_1608: gly
  Search NORINE for peptide: strict or relaxed
  AMP-binding (35..422): gly
  NRPSPredictor2: gly
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      gly, ala, val, leu, ile, abu, iva
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DILQLGcVWK
    Nearest Stachelhaus code:
      gly
    Stachelhaus code match:
      90% (moderate)

NZ_AP022254.1 - Region 3 - RiPP-like
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 3,155,608 - 3,166,450 nt. (total: 10,843 nt) Show pHMM detection rules used
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
MIBiG comparison
KnownClusterBlast
Pfam domains
TIGRFAM domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | RiPP-like | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000506.1 |  | 0.04 | RiPP | entianin | Bacillus subtilis subsp. spizizenii
BGC0001791.1 |  | 0.03 | NRP | sunshinamide | Gynuella sunshinyii YC6258

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000506.1 |  | 0.18 | RiPP | entianin | Bacillus subtilis subsp. spizizenii
BGC0001791.1 |  | 0.16 | NRP | sunshinamide | Gynuella sunshinyii YC6258

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names