LOCUS       NZ_CP023707.1          12512 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP023707.1 Edwardsiella tarda strain KC-Pc-HB1 plasmid pEh-Pc1,
            complete sequence.
ACCESSION   NZ_CP023707
VERSION     NZ_CP023707.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 16:07:48
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 23959
            Orig. end    :: 36471
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12512
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[28959:31471]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7512
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..12512
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12512
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193)"
                     /tool="antismash"
     CDS             complement(487..1038)
                     /locus_tag="ctg2_34"
                     /transl_table=11
                     /translation="MNKICPFLTAAALCVPFAHASGSGIQKTIFNVDVVASACHVRVEA
                     DGTGNSSRLMFGTYRKSTGVLVPPRDFTVRLYEQGATVQGCSAFRAGQVATLSFGNPGQ
                     LDAGGVVTRGAGDGIRVDVRAVDAEADYRGRLTLDNHSVNYPVDFAARGQFRFRAQPEF
                     PKEVKAGEYSGALTFVVTYQ"
     CDS             1272..2087
                     /locus_tag="ctg2_35"
                     /transl_table=11
                     /translation="MLKTLTINAITQYIEDNLETMPIDINKLINYSGYSRRYLQILFRD
                     NIGIPVGKYIQLRRITRAAILLQLTSLNIAHISEILHYDSQQTFTREFKKNSGYTPLQY
                     RKLKIWTFKNMLGAREVNMNVPIPIIRRLEQKRFYGKKIYYKEIIPPATPTSKQKWSII
                     DLLLSEENNQVLISHKHEPSKMDNRYMYFHAIIWDHPDNCTSSEVLSEGSYAYFSFTGS
                     RDGYKKFIYNVYMSALPFYDLKKKDLYDLEIIKKIDTDMYHFEYFLPIE"
     PFAM_domain     1356..1589
                     /aSDomain="HTH_18"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12833.9"
                     /description="Helix-turn-helix domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_35_0001"
                     /evalue="1.10E-15"
                     /label="ctg2_35"
                     /locus_tag="ctg2_35"
                     /protein_end="106"
                     /protein_start="28"
                     /score="57.7"
                     /tool="antismash"
                     /translation="INYSGYSRRYLQILFRDNIGIPVGKYIQLRRITRAAILLQLTSLN
                     IAHISEILHYDSQQTFTREFKKNSGYTPLQYRK"
     CDS             complement(2701..3135)
                     /locus_tag="ctg2_36"
                     /transl_table=11
                     /translation="VHDLTGRGIGLKVLTGHEATIDTTTAAGKLIFGIFAALAEFEREL
                     IAERTFAGLAPAIARGRKGGRPFKMTPAKLRLVMAAMGQPEIRVNDLCRELSIIRQTLY
                     RHVSPEGKLRPDGVKLLSDLKELRICGMQPWRVNLLALQL"
     PFAM_domain     complement(2947..3135)
                     /aSDomain="Resolvase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00239.23"
                     /description="Resolvase, N terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_36_0002"
                     /evalue="6.00E-12"
                     /label="ctg2_36"
                     /locus_tag="ctg2_36"
                     /protein_end="63"
                     /protein_start="0"
                     /score="45.9"
                     /tool="antismash"
                     /translation="VHDLTGRGIGLKVLTGHEATIDTTTAAGKLIFGIFAALAEFEREL
                     IAERTFAGLAPAIARGRK"
     CDS             complement(3521..3757)
                     /locus_tag="ctg2_37"
                     /transl_table=11
                     /translation="VYARSTPLSPHQQRARRAVILEREGYFCAAAEAWSDVAAQAPCVN
                     WQAWARQRASLCSQRDRGQQFRGLSRVVSENRK"
     CDS             complement(3862..4797)
                     /locus_tag="ctg2_38"
                     /transl_table=11
                     /translation="MKKHPATPHDSVFKAFLTHPETARDFLSIHLPPALREICDLDTLQ
                     LAPGSFVEESLRPYYSDVLYTLKTQGGDGFIYTLIEHQSRPDKHMAFRLMRYAIAAMQQ
                     HLDAGHDTLPLVIPILFYQGQTSPYPYSLSWLQEFANPALARQLYCGDFPLVDITVIPD
                     DDIMQHKRIALLELLQKHIRQRDLAELLNQVVTLLLAGYTTEKQVRSLMNYMFQVGETQ
                     NPEALLTTLASQVPQHGDTLMTIAEQLEQKGMQKGMQQGRQEGRVEGLVEGRQEAQREM
                     ARNMLAIGIDHATIMQVTGLTAAELEQVRH"
     aSDomain        complement(3871..4776)
                     /aSDomain="TIGR01784"
                     /aSTool="tigrfam"
                     /description="T_den_put_tspse: conserved hypothetical
                     protein"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg2_38_0001"
                     /evalue="6.90E-64"
                     /identifier="TIGR01784"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="309"
                     /protein_start="7"
                     /score="214.2"
                     /tool="antismash"
                     /translation="PHDSVFKAFLTHPETARDFLSIHLPPALREICDLDTLQLAPGSFV
                     EESLRPYYSDVLYTLKTQGGDGFIYTLIEHQSRPDKHMAFRLMRYAIAAMQQHLDAGHD
                     TLPLVIPILFYQGQTSPYPYSLSWLQEFANPALARQLYCGDFPLVDITVIPDDDIMQHK
                     RIALLELLQKHIRQRDLAELLNQVVTLLLAGYTTEKQVRSLMNYMFQVGETQNPEALLT
                     TLASQVPQHGDTLMTIAEQLEQKGMQKGMQQGRQEGRVEGLVEGRQEAQREMARNMLAI
                     GIDHATIMQVTGLTAAELEQV"
     PFAM_domain     complement(4174..4779)
                     /aSDomain="Transposase_31"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04754.14"
                     /description="Putative transposase, YhgA-like"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_38_0003"
                     /evalue="2.30E-87"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="208"
                     /protein_start="6"
                     /score="292.0"
                     /tool="antismash"
                     /translation="TPHDSVFKAFLTHPETARDFLSIHLPPALREICDLDTLQLAPGSF
                     VEESLRPYYSDVLYTLKTQGGDGFIYTLIEHQSRPDKHMAFRLMRYAIAAMQQHLDAGH
                     DTLPLVIPILFYQGQTSPYPYSLSWLQEFANPALARQLYCGDFPLVDITVIPDDDIMQH
                     KRIALLELLQKHIRQRDLAELLNQVVTLLLAGYTTEKQV"
     CDS             complement(5001..5243)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_39"
                     /sec_met_domain="Cloacin (E-value: 2.5e-17, bitscore: 55.8,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDALQTGCVHINTHDAVIRFPDNSNVKPVYVSVSDVLTPEQVKQR
                     EEEERRRQQMWNASYPLLIWPVSSASRNRIHNMVR"
     PFAM_domain     complement(5073..5213)
                     /aSDomain="Cloacin"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03515.16"
                     /description="Colicin-like bacteriocin tRNase domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_39_0004"
                     /evalue="3.20E-16"
                     /label="ctg2_39"
                     /locus_tag="ctg2_39"
                     /protein_end="57"
                     /protein_start="10"
                     /score="59.9"
                     /tool="antismash"
                     /translation="INTHDAVIRFPDNSNVKPVYVSVSDVLTPEQVKQREEEERRRQQM
                     WN"
     CDS             complement(5483..6502)
                     /gene_functions="other (smcogs) SMCOG1242:transposase
                     (Score: 339; E-value: 4.6e-103)"
                     /gene_kind="other"
                     /locus_tag="ctg2_40"
                     /transl_table=11
                     /translation="MKCTPVGVDIAKHLIQVHFINEHTGEVVDKQLRSRDFLAFFSNRE
                     PCLIGMEACGGSQHWARELKKLGHEVRLMQARFVKAFVMGNKNDVMDARAIWMAVQQPG
                     KAISVKTEEQQSVLVLHHTRRQLVKFRTAQINSLHGTLLEFGETIHKSRAALERELPAA
                     LERMKIRLPPYLISVLEDQYNRLSELDSLIDGLEKQLNSVARQNETCKRLMEISGVGPL
                     IATAAVATMGEASAFKSGRAFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGA
                     RAASLRAKEPGSWITELRKRRPTSVAIVAMANKLARTVWAIAAHERKYDKNYVSVRPY"
     PFAM_domain     complement(5636..5875)
                     /aSDomain="Transposase_20"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02371.18"
                     /description="Transposase IS116/IS110/IS902 family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_40_0006"
                     /evalue="3.10E-22"
                     /label="ctg2_40"
                     /locus_tag="ctg2_40"
                     /protein_end="289"
                     /protein_start="209"
                     /score="78.7"
                     /tool="antismash"
                     /translation="CKRLMEISGVGPLIATAAVATMGEASAFKSGRAFAAYVGLVPKQT
                     GSGGKVRLLGISKRGDTYLRTLFIHGARAASLRAK"
     PFAM_domain     complement(6071..6487)
                     /aSDomain="DEDD_Tnp_IS110"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01548.19"
                     /description="Transposase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_40_0005"
                     /evalue="7.60E-10"
                     /label="ctg2_40"
                     /locus_tag="ctg2_40"
                     /protein_end="144"
                     /protein_start="5"
                     /score="38.9"
                     /tool="antismash"
                     /translation="VGVDIAKHLIQVHFINEHTGEVVDKQLRSRDFLAFFSNREPCLIG
                     MEACGGSQHWARELKKLGHEVRLMQARFVKAFVMGNKNDVMDARAIWMAVQQPGKAISV
                     KTEEQQSVLVLHHTRRQLVKFRTAQINSLHGTLLE"
     CDS             complement(6601..7512)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Cloacin"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_41"
                     /sec_met_domain="Cloacin (E-value: 2.3e-54, bitscore:
                     176.7, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGNGGNNAHGNAFGGGRNPGVGGNHGGDRGSTGGYDGKNLGGHT
                     RDNGDGTSTTTDHRGSITHYGGRERPDAGWSRGGNGGDKDHASSGVNLSLFPEAQANVA
                     YGVPMNISLIDGMWGFSLFRTAPIQKALLDAMAKLEQGLVAGLPYAGRLMGVTFGVLIP
                     SEIAKDDPNMMSQIMTSLPADKVLEAPLAPSLPLQPATVRVKNRVADIIQDEKQKLAVV
                     AGVPMSVPVVEAKPTSRPGVFTASIVPGKPDIHLSIKNNTPVSVSQPKGIPTERGDEKP
                     VGFSAGGNAMNASHFLSTPDDG"
     PFAM_domain     complement(6637..7365)
                     /aSDomain="Cloacin"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03515.16"
                     /description="Colicin-like bacteriocin tRNase domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_41_0007"
                     /evalue="4.60E-47"
                     /label="ctg2_41"
                     /locus_tag="ctg2_41"
                     /protein_end="292"
                     /protein_start="49"
                     /score="161.1"
                     /tool="antismash"
                     /translation="DGTSTTTDHRGSITHYGGRERPDAGWSRGGNGGDKDHASSGVNLS
                     LFPEAQANVAYGVPMNISLIDGMWGFSLFRTAPIQKALLDAMAKLEQGLVAGLPYAGRL
                     MGVTFGVLIPSEIAKDDPNMMSQIMTSLPADKVLEAPLAPSLPLQPATVRVKNRVADII
                     QDEKQKLAVVAGVPMSVPVVEAKPTSRPGVFTASIVPGKPDIHLSIKNNTPVSVSQPKG
                     IPTERGDEKPVGFSAGGNAMN"
     CDS             complement(8584..9663)
                     /locus_tag="ctg2_42"
                     /transl_table=11
                     /translation="VYSNLSQSIADNLSLSPNRRGAANAARKGQRKIHRRYQPQLCCRV
                     SRGMASKVVARISRHDWNRNGDLIALRQRGYIPYTYRHDPHFVPKPMRISTRSESREAL
                     TVLSQALAAHTDYNPDSDYPFEVMVPFEQIASAMGVLHVYESGRKAYDVALHALSVMEQ
                     LGYVIVLHTQDSDSGQNKPLRIWLSETFFTSRGIQVDEIRDWLGKFKRWAMKNGLTETL
                     RQRYEKHLVRIERIGIDLKSRHSLRNRLRQIKRWVVSPDLARDKAQAVAKIEQTLAQRS
                     ALEQQLDETNQNIRQLAKGKKQNSYYQQFTQWSINGGMTTIHVMMLQEALKQEHPGLLQ
                     QDAEAFYKLLLERAGAFKI"
     CDS             complement(9788..10069)
                     /locus_tag="ctg2_43"
                     /transl_table=11
                     /translation="MPPFMHHSDPVTVCTPKQAENRLIFWVVCSMLARSAVSAPSSSSR
                     KRVEDRRQADEFMAGHAARWQGAGEGDVTPRQKSENPDNLFTLAGEED"
     CDS             complement(10069..10635)
                     /locus_tag="ctg2_44"
                     /transl_table=11
                     /translation="MFFIFLGLALFILLFIIWLSAQGGKRDHPLWRALLAAGIKPKESD
                     RLLCQQCSFTAKPQLMTAREYAFFSALSPATGAHHWYFCPQVRVADIVEVSSAWKKGSR
                     QWWCLFRLISQWHCDVVIIERKTGRIIAAIELDDATHQQVKRIRRDIVLNEIFRQAQIP
                     LWRGDDPEALIQQVHHYLQVRTTAR"
     PFAM_domain     complement(10132..10461)
                     /aSDomain="DUF2726"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF10881.10"
                     /description="Protein of unknown function (DUF2726)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_44_0008"
                     /evalue="8.40E-20"
                     /label="ctg2_44"
                     /locus_tag="ctg2_44"
                     /protein_end="168"
                     /protein_start="58"
                     /score="70.8"
                     /tool="antismash"
                     /translation="QLMTAREYAFFSALSPATGAHHWYFCPQVRVADIVEVSSAWKKGS
                     RQWWCLFRLISQWHCDVVIIERKTGRIIAAIELDDATHQQVKRIRRDIVLNEIFRQAQI
                     PLWRGD"
     CDS             complement(10836..11456)
                     /locus_tag="ctg2_45"
                     /transl_table=11
                     /translation="MNTEKRPTLSLKRKRLVEETPAAAADAAPVVVHRKKVVVVNSPPP
                     WKVKKQKLAAQKQAEAAKRAPTKAPSVTPPPAAPARQDKKCPPKPPKGVVLMPLAEAVA
                     LMKPHWPGLFEGDTPRLLQIGLRDALLADIATRALPLSRKQLRRCLKRITRSTTYLRSM
                     VVGASRYGIDGQPVATITEEEQQYACARIESYQRRKSTSANRI"
     PFAM_domain     complement(10854..11162)
                     /aSDomain="ProQ"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04352.15"
                     /description="ProQ/FINO family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_45_0010"
                     /evalue="5.30E-22"
                     /label="ctg2_45"
                     /locus_tag="ctg2_45"
                     /protein_end="201"
                     /protein_start="98"
                     /score="77.9"
                     /tool="antismash"
                     /translation="LAEAVALMKPHWPGLFEGDTPRLLQIGLRDALLADIATRALPLSR
                     KQLRRCLKRITRSTTYLRSMVVGASRYGIDGQPVATITEEEQQYACARIESYQRRKST"
     PFAM_domain     complement(11262..11456)
                     /aSDomain="FinO_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12602.10"
                     /description="Fertility inhibition protein N terminal"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_45_0009"
                     /evalue="3.40E-14"
                     /label="ctg2_45"
                     /locus_tag="ctg2_45"
                     /protein_end="65"
                     /protein_start="0"
                     /score="53.0"
                     /tool="antismash"
                     /translation="MNTEKRPTLSLKRKRLVEETPAAAADAAPVVVHRKKVVVVNSPPP
                     WKVKKQKLAAQKQAEAAKRA"
     CDS             complement(11471..12196)
                     /locus_tag="ctg2_46"
                     /transl_table=11
                     /translation="MRVDVMPSVFQGLACRWQWSAGQCDVLKGVALLLMVLDHANRIFG
                     LHSEVMLWLGRGAFPLFGLVWGYNLARHAGIRQSNLNALWCWAMIAQPAYLLAGFPWWQ
                     GNILFVFAVTGQALRWFEQRQGIKGILAATGLTLIWLPFSLTSYDLAGVVMLVACRYLF
                     VPMAAAERRLVALLWGLSVLMLNVNIGWAAAVSGLVLSVAVAQGGLRWSGQGRFWPSHF
                     FVVTYTVHLAVLSCVASVM"
     PFAM_domain     complement(11486..12127)
                     /aSDomain="TraX"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF05857.13"
                     /description="TraX protein"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_46_0011"
                     /evalue="3.10E-44"
                     /label="ctg2_46"
                     /locus_tag="ctg2_46"
                     /protein_end="237"
                     /protein_start="23"
                     /score="151.7"
                     /tool="antismash"
                     /translation="CDVLKGVALLLMVLDHANRIFGLHSEVMLWLGRGAFPLFGLVWGY
                     NLARHAGIRQSNLNALWCWAMIAQPAYLLAGFPWWQGNILFVFAVTGQALRWFEQRQGI
                     KGILAATGLTLIWLPFSLTSYDLAGVVMLVACRYLFVPMAAAERRLVALLWGLSVLMLN
                     VNIGWAAAVSGLVLSVAVAQGGLRWSGQGRFWPSHFFVVTYTVHLAVLSCV"
     aSDomain        complement(11486..12145)
                     /aSDomain="TIGR02755"
                     /aSTool="tigrfam"
                     /description="TraX_Ftype: type-F conjugative transfer
                     system pilin acetylase TraX"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg2_46_0002"
                     /evalue="3.60E-68"
                     /identifier="TIGR02755"
                     /label="ctg2_46"
                     /locus_tag="ctg2_46"
                     /protein_end="237"
                     /protein_start="17"
                     /score="228.0"
                     /tool="antismash"
                     /translation="QWSAGQCDVLKGVALLLMVLDHANRIFGLHSEVMLWLGRGAFPLF
                     GLVWGYNLARHAGIRQSNLNALWCWAMIAQPAYLLAGFPWWQGNILFVFAVTGQALRWF
                     EQRQGIKGILAATGLTLIWLPFSLTSYDLAGVVMLVACRYLFVPMAAAERRLVALLWGL
                     SVLMLNVNIGWAAAVSGLVLSVAVAQGGLRWSGQGRFWPSHFFVVTYTVHLAVLSCV"
ORIGIN
        1 gcatcttcac cagacctttc tggggaattg acagcgccag actctgggtg gacacatcaa
       61 aatccacgcc cactgacggc gctttcgtca gcacataaca ctgccgcgtg gcgtcataac
      121 cctcacggaa gtagtctgca ctgacataca ccccggcttt tgtcagccat gcctccggca
      181 gacacagttc tttactatct tccgacgtca catccagtat ttgtttcccg gcatcctttc
      241 cgttcaggga caggtccaca agataacgcc ccggcgcata atcaccgttc agtgctgccc
      301 aaacctccga gttaaccccg gcattaccct gaataaagga catatccagt ttttcactgg
      361 cagcttctgt ggcactggca aacactgcag ataccgccag cgccagtacc gcccgacgga
      421 aaaacacagg aggagagttt tccgcgactg ctcttttttt tacaacgcgc acaatcactc
      481 tctccgttac tgatacgtga ccacaaacgt cagcgcacca ctgtattctc ccgccttcac
      541 ctctttcgga aattccggtt gggcacggaa gcgaaactgt cccctggcgg caaagtccac
      601 cggataattc acggaatggt tatccagcgt caggcgcccg cgataatccg cctctgcatc
      661 aactgcccgt acatccacac ggatgccgtc gccggcgccg cgggtaacca cacctcccgc
      721 atccagctgg cccgggttac caaaactcag ggtggcgacc tgccctgccc gaaaggcaga
      781 acagccctga acagtggcgc cctgctcata cagacgcacc gtgaaatcac gcggcggtac
      841 taatacgccg gtagatttac ggtaagtacc aaacatcaga cgactgctgt tgccggtacc
      901 atccgcctcc acccttacgt ggcaggctga agccacaaca tccacattaa atatcgtttt
      961 ttgaatacca ctcccactcg catgggcaaa aggcacacag agtgcagcag cagtcaaaaa
     1021 gggacagatt ttattcatga ctaatatgct ctcacagcca atgctattac ccagcattgc
     1081 aatttggaat gggcggcaat tttaggaatt ttaagatatt agtattatct aatcgtgcat
     1141 aagctcaaaa tgttgcacaa ttagataaaa aagcaagacg tgttgcatgc agtgcacaat
     1201 tagataaaaa agtaagacat attgccttat gataaccagc caaaatatag tcttcataag
     1261 gaatgcgtag catgttaaaa acacttacaa taaatgccat aactcaatat attgaagata
     1321 atctggagac tatgccaatt gatataaaca aactcattaa ttattctgga tatagcagaa
     1381 ggtatttgca aatattgttt agggataata ttggcattcc cgttggtaaa tatattcagt
     1441 tacgcaggat tacgcgggca gcaattttat tacaacttac gagtcttaat attgcacaca
     1501 tttcagaaat attgcactac gattctcagc aaacttttac gcgggaattc aagaaaaata
     1561 gcggatacac acctttgcaa tatagaaaac ttaaaatttg gacgtttaaa aatatgcttg
     1621 gcgcaagaga ggtaaatatg aatgtcccga tccccatcat tcgacgttta gagcaaaaac
     1681 gtttttacgg taaaaaaata tattataaag aaattatacc tccagccaca cctacatcca
     1741 aacagaaatg gagtattata gatttacttt tatctgaaga aaataaccaa gttttaatat
     1801 cgcacaaaca tgagccatct aagatggata acagatatat gtatttccat gctatcattt
     1861 gggatcaccc tgacaactgc acgtcaagtg aagtgctgag cgaaggctct tatgcgtatt
     1921 tttcgtttac aggttcaagg gatggttata aaaaatttat ttataatgta tatatgtcag
     1981 cattgccatt ttatgacttg aaaaagaaag atttgtacga ccttgagata ataaaaaaaa
     2041 tagacactga tatgtatcac ttcgaatatt ttttaccaat agagtgatgt gtgtacattt
     2101 taccgctccc ccgaatgaaa gtctcacgca aatgattcta ttattaagta aatgcttaca
     2161 catacccaga aaaagcagac acacctcacg tggtataact atttattatc accgaatcgc
     2221 agagaatata attataaaac tagccggacg caccgttagc ggcgcgtgtc aaggtagttg
     2281 tctcctcagt tattcatgcc gccagagttt ctcgttccgg atttagccac acctcttcgt
     2341 gccggctcca gttttgagtc ccccctgacc agcgataggg attccgtatc ttcgcggctt
     2401 catacactga ttttcgttat gccagcagca cgttatcctc ccctctgtgt tgctgaccgg
     2461 gcgtcacgta acggatccct ctgtgtctgt gttcttcgtt gtaccagccg acgaagcttt
     2521 ctacccaagc cctcgcttcc tccaacgtcg agaatcctct ctccggggtt ctacgccgga
     2581 accccagaaa tttccgccac cagaatgttt agaagctaac tggcaaaaat agtgttcgcc
     2641 tgttagtgga gaaagtgatt tatatgaggt cttttgctgc taattgcctg taatgctcac
     2701 ctagagctgt aaggctaaca gatttactct ccatggctgc atgccacata tgcggagctc
     2761 cttcaggtct gatagtagtt tcaccccatc tgggcgcaat tttccttcag gtgaaacatg
     2821 tcggtataac gtttgtcgaa tgatgctcag ttcccggcat agatcgttaa ccctgatttc
     2881 tggttgcccc attgctgcca tcaccagacg taatttagct ggagtcatct tgaaaggacg
     2941 gcctcctttc cgcccgcgag ctatcgctgg ggctaggcct gcaaatgttc gctcggcaat
     3001 cagttcacgc tcaaactcgg ccagagcggc aaagatccca aaaataagct tgccggcggc
     3061 tgtcgtggta tcgatagtcg cttcgtgacc ggtcagtacc ttcaggccga tcccgcgtcc
     3121 agtaaggtca tgcaccgtat taatgagatg acgaaggtcg cgaccgagtc gatccagttt
     3181 ccatacaatc agggtgtcat ctggccgtag ggccttcaga caggcaataa gtccagggcg
     3241 atcttctcgc ttgcccgatg cccggccttc ataaatatgg gcagcgatca gcgcatcacg
     3301 ctgtaaatca ttcgcctgag aaccatctgc tttttatacc cgcatataac caattaacat
     3361 tttacacctg tcacatatac gacccgttga gtgacaacct ttaaaaaaac gatttcgaca
     3421 ctgaatcccg tcacataacc cgtaatttat tcaatatgat gcaaatcagt tggtacttag
     3481 ctttggtgac ggaattttct ggaattccgg catagagccc ctactttctg ttttctgaga
     3541 caacccgaga cagaccacgg aactgttggc cccggtcgcg ctgtgaacac aggcttgcac
     3601 gctgccttgc ccaggcttgc caattaacgc atggcgcttg cgcggccaca tcgctccagg
     3661 cctctgcagc tgcgcagaaa tagccctctc gctcgagaat aacagcgcgt cgtgcacgtt
     3721 gctgatgggg agataagggg gtagaccgag cgtacacgat gaggacctcc ttgcgtctta
     3781 ctgtattttt tctgagttag gcggcccctc tccctaacac taaggcaggg gagagggaaa
     3841 atacctcggc ggcatattct attaatgtcg aacctgctcg agctcagcgg cggttaaacc
     3901 ggtcacctgc ataatcgtag cgtgatcgat accgatggcc agcatgttgc gcgccatttc
     3961 gcgttgggct tcctggcggc cttctacgag gccttctacg cggccttctt ggcggccttg
     4021 ctgcatacct ttctgcatgc ctttctgttc aagttgttca gcgatggtca tcagcgtgtc
     4081 tccatgttgc ggcacttgcg atgccagagt ggttaacagg gcctccgggt tttgtgtctc
     4141 ccccacctgg aacatatagt tcatcaggga gcgcacctgc ttttcggtag tgtagcctgc
     4201 caacagtaac gtgaccacct ggtttagcaa ctcagccagg tcacgctgcc ggatatgctt
     4261 ctgcagcagc tccagcaagg cgatgcgctt gtgctgcatg atgtcgtcat ccgggatgac
     4321 ggtaatatcc accaacggga aatcaccgca gtatagctgc ctggccaacg ccgggttggc
     4381 aaactcctgt agccaggaca ggctgtaggg atacggcgac gtctgcccct ggtagaacag
     4441 gatgggtatc accagaggca atgtgtcgtg gccagcatcg aggtgttgtt gcatggcagc
     4501 aatcgcataa cgcatcaaac gaaatgccat gtgcttgtcc ggtcgggatt gatgctcgat
     4561 caatgtatag ataaaaccgt ccccaccctg ggtcttcagg gtgtacaaca catcactgta
     4621 atacgggcgc aagctctctt cgacaaacga accgggagcc agttgcagcg tatcgaggtc
     4681 acagatttct cgcagtgccg gcggcagatg aatagacaga aaatcccggg cggtctcggg
     4741 gtgagtgagg aacgccttga atacgctgtc gtgaggagtc gctggatgtt ttttcattca
     4801 ggtatattaa cattttaccc tgcctgcaga tagcgggcgg ccatattatc aaacccaaaa
     4861 atcacaacga cccggacgag cgagccatcc tttggatggg ggtagctcag accgctacga
     4921 cctctatctg acttagcgta cgattttctc cattttccaa gcggccccca atactcgatc
     4981 attgttcgaa cgataccctt ctatctcacc atgttgtgaa tcctatttct actggcactt
     5041 gataccggcc atatcagaag aggataagat gcattccaca tctgttggcg acgtcgctct
     5101 tcctcctcac gctgttttac ttgttcaggt gttagcacat ctgatacgga aacataaacc
     5161 ggtttaacat tactattatc aggaaagcgg atcactgcat catgggtgtt tatatgaacg
     5221 catcccgttt gcaaggcatc cattgttttg acattgatgg gtaggttgca gctttatatc
     5281 cggctttttt gcagctataa agctgcgagc cactatgcaa ttcgctgact cacgccttat
     5341 ttcctcagcg ggctttaagc ccactaaccc attcaggttt agtgagtccc ggtcttacct
     5401 gtctttgtca tcactatggc tgccagagca acctttcagc attcaccctt tgttaagagt
     5461 taatgtttat cggtatcagc gattaatacg gtctgacact gacataattt ttatcatatt
     5521 tacgttcatg ggcagctatc gcccatactg ttcgcgccag cttgtttgcc atcgccacta
     5581 tggccacgct ggtcggacgc cgctttctca gttccgttat ccatgaacca ggttcttttg
     5641 cacgtagcga tgctgccctt gccccgtgga taaacaacgt tcgcagatag gtgtcacctc
     5701 gtttgcttat ccccaacaga cggacttttc ctcccgaacc agtttgcttc ggaaccagac
     5761 cgacataagc agcgaatgct ctcccggatt taaacgctga tgcctcaccc atagtggcaa
     5821 ctgcagcggt ggcaattaac gggcccacac cggagatttc catcaggcgc ttacaggttt
     5881 cattctgtcg agccacgctg ttaagctgtt tttccagacc atctatcagc gagtccagtt
     5941 cactcagcct gttgtactgg tcttcaagta cactgataag atacggtggc aaccttatct
     6001 tcatccgttc cagtgctgcc ggtagctctc tttccaacgc tgcccggctt ttgtggatag
     6061 tctcgccaaa ctccagtaat gttccgtgaa gggagttaat ctgcgcggtg cgaaacttca
     6121 ccaactgcct gcgagtgtga tgcaggacca gcaccgactg ctgttcctcc gttttaacgg
     6181 agatcgcttt accgggttgc tgcaccgcca tccagatagc cctggcatcc atcacatcat
     6241 tcttgttgcc catgacgaag gctttaacga aacgagcctg catcaaccgg acttcatgtc
     6301 ccagtttttt aagctcgcga gcccagtgct gagagccacc acaagcctcc atgccaatca
     6361 ggcagggttc ccggttactg aagaacgcca gaaaatctct gctgcgtagc tgcttatcca
     6421 ccacttcacc cgtatgctca ttgatgaagt gaacctgaat taaatgcttt gcgatatcaa
     6481 cgccaaccgg tgtacatttc atgctgtgga gtctcctgtc tgagggggct ttatgcatcc
     6541 cgtattgggc acaatgatgc cggaaatctg cgaggctcca cacctgccaa cttcactctt
     6601 tcaaccatca tctggtgtgc ttaagaagtg ggatgcgttc attgcatttc ctcccgcact
     6661 aaaccccaca ggtttttcat caccccgttc agtaggaata cctttaggct ggctaacgga
     6721 tacaggcgta ttattcttaa tactcagatg gatatccggc ttaccaggta caatactggc
     6781 tgtaaaaaca ccaggacggg aggttggctt agcctcaacc acagggacac tcattggtac
     6841 accagctacc actgccagct tttgcttttc atcctgaatg atatccgcca cccgattttt
     6901 tacccggaca gtagctggct gtagaggtaa tgagggtgcc aatggtgcct ctaacacttt
     6961 atctgccgga agagatgtca taatttgaga catcatatta ggatcatctt ttgcgatttc
     7021 agaggggatt aacacaccaa aagttacccc cattaatcgc ccagcataag gcagaccggc
     7081 aactaaaccc tgctccagct ttgccattgc gtccaataaa gctttctgaa ttggtgcggt
     7141 acgaaacaga gaaaatcccc acataccatc aataagagaa atattcatcg gcacgccata
     7201 agcgacattg gcttgagcct cagggaataa tgacagatta acgccagagc tagcgtgatc
     7261 tttatcacca ccatttccac cccgactcca tccggcatcg ggacgttccc gtccaccata
     7321 atgagtaata gagcctcgat gatctgttgt cgttgaggtc ccatcaccat tatcacgagt
     7381 atgcccgcct aaattcttcc catcgtaccc accggtggac cctcggtctc caccatgatt
     7441 accccctaca ccagggtttc ggcctccacc aaaagcatta ccgtgagcat tattgccccc
     7501 atttccactc ataacgattc ctctttgaca tataaaaacc aaaaataaac actgtatata
     7561 aacacatgta tttatataca gtgtttatgc tagctctacg atagttagct gtcaatgcaa
     7621 ttaatttgat tgcaatcaaa atttcaaccg atgtgaatgg gagggagcgt tcagtcaacc
     7681 ccttgatcca atgagtgagc cgtgcgcaag tagcatcttt ggatgccgca aggcggcatg
     7741 gaggcgccaa cagatgttga atagaacact gtcagtgact ggcgccgtcg cccggcgcag
     7801 ccggttactg tcttgatagg tttcacctta cccatccctt gccctgctaa gccacaatcc
     7861 gctaacagag agcgtagacg tgaatgtgcg ccgtcggagt ggtcacgaca aggcgtagcc
     7921 gtagtcgggg cgtatctccg cgttagcggg ccgttagggc cgcttacggg cgtgttatac
     7981 cgaatcttcc aggcatagca ttttatccgg cagcgattaa ttttagttac aacacccatc
     8041 attaaaagcg acatcatccc ggcccagagg gccggggcgg cggcgctttt cagtggtggg
     8101 atgggcgttg gtgtttccgc tgggcgcggt actgatgatg agtttggcgg tggtgtttag
     8161 ctggacgtag tttggggccc atcgcaacgc gatgggaggt actttttaaa tgtcgttgcc
     8221 ggttggcttt ggtgttgcca gggaagggtt tgtgcccgag tgaaacgagg gagctaccat
     8281 cgagagcagc cgtaaggcta gctcgatgct tactcccttt tttaaaaggg cttttgttca
     8341 gaaacaacgt tcgaaattaa tgatgatgtt aggtacctct tcgaagttaa tctgttactt
     8401 cgaatttaac tgtctatttc ctgtgctata gcggtaggcc acgttggcca tcaccgaaaa
     8461 tccgtgaata tcgaagagag agggggagaa taacgctcaa aatctgtgct actacccaat
     8521 cgcagggcaa gcgagggcct cgcggtgata ggcgaaaacg tgagggatta aaggggggaa
     8581 tgtttagatt ttaaaggcgc cggcacgttc aagcagcaac ttgtagaacg cctccgcgtc
     8641 ctgctgcagc agtccagggt gctcttgttt aagcgcctcc tgaagcatca tcacgtgaat
     8701 ggtcgtcata ccaccgttta ttgaccactg cgtaaactgc tggtagtagc tgttttgttt
     8761 tttacccttg gccagctgcc ggatgttctg attggtctca tccagttgtt gctccagcgc
     8821 cgagcgttgc gccaaggttt gctctatctt ggccacggcc tgagccttat cacgcgccaa
     8881 gtccgggctg accacccagc gcttgatttg gcgcagccgg ttacgcaatg agtgacggga
     8941 tttcaggtca ataccgatac gctcaatgcg caccaggtgt ttttcatagc gttggcgcag
     9001 tgtctctgtc aggccgttct tcatcgccca gcgcttgaat ttgcccaacc agtcgcgtat
     9061 ttcatcgacc tggataccgc gcgaggtaaa gaacgtctcg gatagccaga tacgcagcgg
     9121 cttgttctga ccactgtcgc tgtcctgggt atgcagcaca atgacatagc ccagctgctc
     9181 catcactgag agggcatgca aggcaacgtc gtaagctttg cgaccgctct cgtaaacgtg
     9241 cagtaccccc atcgccgagg cgatttgctc aaacggcacc atcacttcga agggatagtc
     9301 gctatccggg ttgtagtccg tgtgtgcagc caacgcctgg gagagaacag ttaacgcttc
     9361 acggctctct gagcgcgtgc tgatccgcat cggtttgggc acaaaatgcg gatcatgacg
     9421 ataggtatag ggaatatatc cccgctggcg cagggcaatc agatcgccat tgcgattcca
     9481 atcgtgacgg ctaatccggg cgaccacttt agaggccatc ccgcgcgata cgcgacaaca
     9541 cagctgaggc tgatagcggc gatggatttt acgctgcccc ttacgggccg cattggccgc
     9601 gccccgccgg tttggcgaga gggacaggtt atcggcaatc gactgagaaa ggttgctata
     9661 caccccgccc tgcgcttatg ttgcgcggac agagtacgga gcacagaaag gattgcagtt
     9721 ttctgagcat gatcttatac tccctgcata gagcaacagc ttctatgcag tatctgagtg
     9781 agccccgtta atcttcctcg ccggccaaag tgaagagatt atcggggttt tcgcttttct
     9841 ggcgcggagt aacatccccc tcaccagctc cctgccacct tgcggcgtgg cccgccataa
     9901 attcgtctgc ctgtctcctg tcctcgaccc tcttcctcga tgatgatgag ggggcagaaa
     9961 cagcgcttct cgcaagcata ctacagacaa cccaaaaaat taacctgttt tcggcctgtt
    10021 ttggcgtaca gacggtaaca ggatcagaat gatgcataaa aggcggcatc accttgcagt
    10081 cgttcgaacc tgaagataat ggtgtacctg ctggatgagc gcctcgggat catctcctcg
    10141 ccataacggt atctgtgcct gacgaaaaat ttcgttgagc acaatatcgc gccgtatacg
    10201 ttttacttgt tgatgcgttg catcatccag ctcaatcgcc gcaataatgc gacctgtttt
    10261 tcgctcaata atcacgacat cacaatgcca ctgtgaaata agccggaata aacaccacca
    10321 ttgacggcta ccttttttcc aggctgacga aacctcaaca atatcggcga cacgcacctg
    10381 gggacaaaaa taccagtgat gcgcgccagt cgccggcgat aacgcggaaa agaaggcata
    10441 ctcacgcgcg gtcatcaatt gtggcttggc cgtaaatgaa cactgctgac acagtaatct
    10501 atccgattct ttcggcttga tgcccgccgc cagcaaggcg cgccataagg gatgatcccg
    10561 ttttcctcct tgcgccgata gccagataat aaataataat ataaataggg caagccctaa
    10621 aaagataaag aacatgatcg ctctccgatt ggccgctatg ttgccaaata ataaagcgac
    10681 aatggcaatc ataaaataac ggcagtaata atttcattat gaaaacttac attttgtaaa
    10741 atgcaagaaa actaacgccc tacggttgtt caattgcgtt cactccccga tattaaatca
    10801 gggcgcgaac acaattttat aaaatataaa taaaactaaa tgcggttagc ggaggttgat
    10861 tttcgccgtt gataggattc aattcgggcg cacgcatact gctgctcctc ctccgtgatc
    10921 gtggcaaccg gctggccatc aatgccatag cgggaagcac caacaaccat actgcgcaaa
    10981 taggtcgtcg agcgcgtaat acgtttcagg cagcggcgca gctgtttgcg tgacaagggc
    11041 agcgcgcgcg tagcaatgtc ggccagcaac gcatcccgta acccgatttg cagcaaacgc
    11101 ggcgtgtctc cctcaaacaa ccctggccag tggggtttca tcagggcaac ggcctccgcc
    11161 aatggcatca ataccacccc tttcggcggc ttcggtgggc attttttatc ttgcctggcc
    11221 ggggcggcgg gtggcggcgt gacagacggc gcttttgtcg gcgccctttt cgccgcctcc
    11281 gcctgctttt gtgccgccag tttctgcttc ttcaccttcc agggaggtgg cgagttcacc
    11341 accaccactt tcttgcgatg gacaaccacc ggagcggcat ccgctgccgc agcaggtgtt
    11401 tcttcaacga gtcgtttacg cttcagactc aatgttggcc gcttttctgt attcatctaa
    11461 ttctccctgt tcacatcaca ctggccacac aactcaatac agccagatgt accgtatacg
    11521 tcaccacaaa aaaatgtgag ggccaaaacc gcccttgacc agaccatcgc aggcccccct
    11581 gcgcgacggc tacactcagc accaggccac tcaccgccgc tgcccagccg atattaacat
    11641 tcaacatcag aacactcagt ccccacagca gggcaaccag ccgacgctcc gctgctgcca
    11701 ttggcacaaa aaggtagcga caggccacta acatcacgac gcccgccaga tcgtagcttg
    11761 ttagtgagaa gggaagccaa atcagggtaa gccctgtcgc tgccagtatc cccttgatcc
    11821 cctgtcgttg ctcaaaccag cgtaaggctt gcccggtgac cgcgaacaca aacaggatat
    11881 tcccttgcca ccacgggaac ccggccaaca gatacgcagg ctgggcgatc atcgcccaac
    11941 accacagggc gttgagattc gactggcgta tgccggcatg cctggccaga ttataccccc
    12001 acaccagccc aaacagcgga aaggcccccc gccccagcca cagcatgacc tcactgtgca
    12061 agccaaagat ccggttagca tggtcaagca ccatcaacaa taatgcgacc cctttgagca
    12121 catcacattg cccagcactc cactgccagc gacaggccag tccctgaaaa actgacggca
    12181 tcacatccac tctcattgaa cgtctcctct tggcctgggg cggcaccctg attttaatcg
    12241 gggtgtttcc ccaggctgac aaaccgcatc gcggtgcgtc agtccccggc cagggtcttg
    12301 tcgcgtacca gctcacgctc catctgctgt aagcgctccc gctgtaaacg ctcggcaacc
    12361 tgctcaacgg cggactgttg atgatccggt aaggtcggca gcgtatccgc cggatgcggc
    12421 acctgctcag cgccgccttt gaccacctca tcgacaatct gccgcatttt ctctggcgcc
    12481 acatcatcgg gtgagggttt ctctttttgc cc
//
