LOCUS       NZ_CP060401.1          10795 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP060401.1 Xenorhabdus nematophila strain SII chromosome,
            complete genome.
ACCESSION   NZ_CP060401
VERSION     NZ_CP060401.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 14:15:16
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 2728473
            Orig. end    :: 2739268
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10795
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[2733473:2734268](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5795
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..10795
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10795
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     CDS             complement(385..855)
                     /gene_functions="other (smcogs) SMCOG1238:transposase
                     (Score: 117.2; E-value: 1.6e-35)"
                     /gene_kind="other"
                     /locus_tag="ctg1_2352"
                     /transl_table=11
                     /translation="MKIHLTEDQKKALELMHDTTRDSRVCDRIKAVLLASEGWTTQRIA
                     QALRIPESTVSRHLKDYFSEEKLAPENGGSESRLLAEQATELIEYLTANLMHTTVQIVA
                     YVWARWQVTFTVSGMTKWLHRQGFSYKKSMGVPHKFNADKQPQFIETYTNLT"
     PFAM_domain     complement(400..570)
                     /aSDomain="HTH_33"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13592.8"
                     /description="Winged helix-turn helix"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2352_0870"
                     /evalue="2.00E-17"
                     /label="ctg1_2352"
                     /locus_tag="ctg1_2352"
                     /protein_end="152"
                     /protein_start="95"
                     /score="62.7"
                     /tool="antismash"
                     /translation="MHTTVQIVAYVWARWQVTFTVSGMTKWLHRQGFSYKKSMGVPHKF
                     NADKQPQFIETY"
     PFAM_domain     complement(664..786)
                     /aSDomain="HTH_23"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13384.8"
                     /description="Homeodomain-like domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2352_0869"
                     /evalue="1.00E-09"
                     /label="ctg1_2352"
                     /locus_tag="ctg1_2352"
                     /protein_end="64"
                     /protein_start="23"
                     /score="38.1"
                     /tool="antismash"
                     /translation="RVCDRIKAVLLASEGWTTQRIAQALRIPESTVSRHLKDYFS"
     CDS             908..1498
                     /gene_functions="other (smcogs) SMCOG1238:transposase
                     (Score: 182.3; E-value: 2.2e-55)"
                     /gene_kind="other"
                     /locus_tag="ctg1_2353"
                     /transl_table=11
                     /translation="VVYNALKEKCGQNEPILFIDAVHPTQSTKLSYGWMKSGRKHVKVV
                     ETTGSRTRLNIMGALNLHQIENTIIREYPTINAKNVVLFFGAIRETYPLSQRIHLILDG
                     AGYHRSELVQFFAEVLNIELHYLPPYSPNLNPIERLWKYMNEQVHNNVYFPDVKTFRET
                     LHHFFHVVLPKKAQELATRLTDNFQTLKPASSS"
     PFAM_domain     953..1390
                     /aSDomain="DDE_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13358.8"
                     /description="DDE superfamily endonuclease"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2353_0871"
                     /evalue="2.40E-39"
                     /label="ctg1_2353"
                     /locus_tag="ctg1_2353"
                     /protein_end="161"
                     /protein_start="15"
                     /score="134.5"
                     /tool="antismash"
                     /translation="ILFIDAVHPTQSTKLSYGWMKSGRKHVKVVETTGSRTRLNIMGAL
                     NLHQIENTIIREYPTINAKNVVLFFGAIRETYPLSQRIHLILDGAGYHRSELVQFFAEV
                     LNIELHYLPPYSPNLNPIERLWKYMNEQVHNNVYFPDVKTFR"
     CDS             1925..2713
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 55.8;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2354"
                     /sec_met_domain="Abhydrolase_6 (E-value: 7.4e-08, bitscore:
                     31.1, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 6.7e-09, bitscore: 33.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEISCNGSTVKYRIHAAKSTENAEKIMFLHGTNSDGNSSFSNVK
                     NEFIDKLDVVIPDYAGCGESTLPCSLDVEGLADQIISIIENCNFDKPIHLVGISLGAVV
                     AAVVAAKRPSFVNKLILTAPWASSDDIRFTFMFKTWYELELKDKRMATAFGLSHVLSPK
                     KLSDLSNETIDMICNKESKNIEERIKIGLNINITEYLKEIKSPTLVLGLSYDNLIPTYM
                     VKKVSSLIPNSIYDEIESGHAVQIENPSIWIDKIKEFIEY"
     PFAM_domain     2006..2302
                     /aSDomain="Abhydrolase_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00561.22"
                     /description="alpha/beta hydrolase fold"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2354_0872"
                     /evalue="3.70E-08"
                     /label="ctg1_2354"
                     /locus_tag="ctg1_2354"
                     /protein_end="126"
                     /protein_start="27"
                     /score="33.4"
                     /tool="antismash"
                     /translation="MFLHGTNSDGNSSFSNVKNEFIDKLDVVIPDYAGCGESTLPCSLD
                     VEGLADQIISIIENCNFDKPIHLVGISLGAVVAAVVAAKRPSFVNKLILTAPWA"
     CDS             2862..3023
                     /locus_tag="ctg1_2355"
                     /transl_table=11
                     /translation="MRFDNISIIGNGVSGIATFISLVMYSNIKIITFISPTEMGGQKHS
                     LMLNSCSK"
     CDS             3106..4137
                     /gene_functions="other (smcogs) SMCOG1229:transposase
                     (Score: 216.9; E-value: 6.6e-66)"
                     /gene_kind="other"
                     /locus_tag="ctg1_2356"
                     /transl_table=11
                     /translation="MPIIASIPRNERRLMQKTIQKTRDKNHARRLIAMLMLYRGDTVSY
                     VAKTLACSRSSVGRWINRYTLYGLEGLKSRPPGRPRRWPFELICSLLRHLISDSPDVLG
                     YQRSRWSSELLAIQINEITGCSLHASTIRRWLPQANIVWRRAVPTLRIRDPHKEEKMRA
                     INDALERCSPDHPVFYEDEVDIHLNPKIGADWQLRGQQKRITTPGQNEKYYLAGALHSG
                     TGKGSYVGGNNKDSSLFIKLLAHLKAAYRRAKSITLIADNYIIHKSQKTQEWLAENPKF
                     KLIYQPIYSPWINHIEKLWLALHETITRNHCCCYMWQLLRKVRGFMNTVSPFPGNKHGT
                     AKV"
     PFAM_domain     3190..3348
                     /aSDomain="HTH_28"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13518.8"
                     /description="Helix-turn-helix domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2356_0873"
                     /evalue="7.80E-09"
                     /label="ctg1_2356"
                     /locus_tag="ctg1_2356"
                     /protein_end="81"
                     /protein_start="28"
                     /score="35.6"
                     /tool="antismash"
                     /translation="RRLIAMLMLYRGDTVSYVAKTLACSRSSVGRWINRYTLYGLEGLK
                     SRPPGRPR"
     PFAM_domain     3634..4032
                     /aSDomain="DDE_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13358.8"
                     /description="DDE superfamily endonuclease"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2356_0874"
                     /evalue="2.00E-22"
                     /label="ctg1_2356"
                     /locus_tag="ctg1_2356"
                     /protein_end="309"
                     /protein_start="176"
                     /score="79.7"
                     /tool="antismash"
                     /translation="VFYEDEVDIHLNPKIGADWQLRGQQKRITTPGQNEKYYLAGALHS
                     GTGKGSYVGGNNKDSSLFIKLLAHLKAAYRRAKSITLIADNYIIHKSQKTQEWLAENPK
                     FKLIYQPIYSPWINHIEKLWLALHETITR"
     CDS             4369..4983
                     /locus_tag="ctg1_2357"
                     /transl_table=11
                     /translation="MFSKYVLITRFPLKEKVNMKKLNSLEMNSNNRYFISEEKDMNELL
                     ELRSFSSISEIEEIENEIELDFIKFSSILRGDIRRELVKFVEAPIDSENELPMTKYIQL
                     RHVEVPPENYNRYRQWRDETIFNVVRENKDKITSFGAYHSFISGQPGVMFISSFNVNKE
                     KYLESFTNEHYQNIIKQAGDNYITGGNDGLYTRIYRSIQDH"
     CDS             5001..5795
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_2358"
                     /sec_met_domain="AfsA (E-value: 4.9e-11, bitscore: 40.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNITSCKKTIVVGDKFNEFTNGKDAITISQLDLITQIPAHIMDT
                     NKQFVIGQGVRHDFAEKILENKKNNNYNNKELDLENIQKFVCYEKNKYSHKRIPHNTII
                     GSTRKENENSFSLPLLIDERCELMSDHQTGQHIQGMILIEACRQTFIAVTEEFFMSEKI
                     GQSYYVINGMDIEFSNFLFPLPAMINYKVIDKDINERRSRFKADIHILQHTKHCASMTV
                     SFTVYPKKVISEKESMMADEITQAILRSQHITTQTFKENVYA"
     PFAM_domain     5283..5675
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2358_0875"
                     /evalue="1.70E-14"
                     /label="ctg1_2358"
                     /locus_tag="ctg1_2358"
                     /protein_end="225"
                     /protein_start="94"
                     /score="54.0"
                     /tool="antismash"
                     /translation="HKRIPHNTIIGSTRKENENSFSLPLLIDERCELMSDHQTGQHIQG
                     MILIEACRQTFIAVTEEFFMSEKIGQSYYVINGMDIEFSNFLFPLPAMINYKVIDKDIN
                     ERRSRFKADIHILQHTKHCASMTVSFT"
     CDS             5788..6621
                     /locus_tag="ctg1_2359"
                     /transl_table=11
                     /translation="MPEKYGQTYNLAVTTQGPIYPPSEVVDAYGNFVVIGQIATDEGIY
                     WGCAIVDKNSPLPPYGNIKSYHIIQNLNDLSEESLEKIVLYTLPLPLPMNNYPMIFAPE
                     QRPNTVHDKRSSRPLHEGYIADFREVDGRRKLQPITLKDWMKAKGTLTVTLSKDKRSAR
                     FDFTFNHLIPHSVYTVMSLRERDLAPKNPSRPGPLGIPNVFITDDNGNATYWAELPNPF
                     PVKGEERNRVINIVVLFMSCQQSFGGAIGLYGLGGDIHAHLKLPAASFSEFYTME"
     CDS             6693..7349
                     /locus_tag="ctg1_2360"
                     /transl_table=11
                     /translation="MSKIAAFFDVDETLIKIKSMFHFYQFWCYQKKRQDEYNSFNKQFI
                     NAISKGTEREELNRMYYRQLKGVHIDELYQAGEYWFNKHLQKDEIYIQSAINEYFNHKK
                     LEHLTIFVSGSMLPLLKPLGKYLDVDFILCTKLLLDKEDRLTGEIGEPQTIGNGKKESI
                     IKFSYKNNINLKNSFAYGDDISDIQMLESTGNPICIGNNKELIKHAEINNWKTII"
     aSDomain        6705..7268
                     /aSDomain="TIGR01488"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB: HAD phosphoserine phosphatase-like
                     hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2360_0202"
                     /evalue="7.80E-22"
                     /identifier="TIGR01488"
                     /label="ctg1_2360"
                     /locus_tag="ctg1_2360"
                     /protein_end="192"
                     /protein_start="4"
                     /score="76.0"
                     /tool="antismash"
                     /translation="AAFFDVDETLIKIKSMFHFYQFWCYQKKRQDEYNSFNKQFINAIS
                     KGTEREELNRMYYRQLKGVHIDELYQAGEYWFNKHLQKDEIYIQSAINEYFNHKKLEHL
                     TIFVSGSMLPLLKPLGKYLDVDFILCTKLLLDKEDRLTGEIGEPQTIGNGKKESIIKFS
                     YKNNINLKNSFAYGDDISDIQMLES"
     aSDomain        6705..7301
                     /aSDomain="TIGR01490"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB-hyp1: HAD hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2360_0201"
                     /evalue="4.70E-33"
                     /identifier="TIGR01490"
                     /label="ctg1_2360"
                     /locus_tag="ctg1_2360"
                     /protein_end="203"
                     /protein_start="4"
                     /score="113.1"
                     /tool="antismash"
                     /translation="AAFFDVDETLIKIKSMFHFYQFWCYQKKRQDEYNSFNKQFINAIS
                     KGTEREELNRMYYRQLKGVHIDELYQAGEYWFNKHLQKDEIYIQSAINEYFNHKKLEHL
                     TIFVSGSMLPLLKPLGKYLDVDFILCTKLLLDKEDRLTGEIGEPQTIGNGKKESIIKFS
                     YKNNINLKNSFAYGDDISDIQMLESTGNPICIGNNK"
     PFAM_domain     6708..7265
                     /aSDomain="HAD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12710.9"
                     /description="haloacid dehalogenase-like hydrolase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2360_0876"
                     /evalue="6.50E-21"
                     /label="ctg1_2360"
                     /locus_tag="ctg1_2360"
                     /protein_end="191"
                     /protein_start="5"
                     /score="75.8"
                     /tool="antismash"
                     /translation="AFFDVDETLIKIKSMFHFYQFWCYQKKRQDEYNSFNKQFINAISK
                     GTEREELNRMYYRQLKGVHIDELYQAGEYWFNKHLQKDEIYIQSAINEYFNHKKLEHLT
                     IFVSGSMLPLLKPLGKYLDVDFILCTKLLLDKEDRLTGEIGEPQTIGNGKKESIIKFSY
                     KNNINLKNSFAYGDDISDIQMLE"
     CDS             7778..8188
                     /locus_tag="ctg1_2361"
                     /transl_table=11
                     /translation="MKSSKTLTEHGRYIMELLEPGLADKIMENLSELDSDLPFLITDYA
                     FGAILGREGLNLKAREMISVASLITLGNAQPQLELHMRAALNVGVTEKELLEIIIQMAV
                     YAGIPACMNGLTAFRAAVAQKNTLNTKTGEKL"
     PFAM_domain     7898..8134
                     /aSDomain="CMD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02627.22"
                     /description="Carboxymuconolactone decarboxylase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2361_0877"
                     /evalue="5.80E-17"
                     /label="ctg1_2361"
                     /locus_tag="ctg1_2361"
                     /protein_end="119"
                     /protein_start="40"
                     /score="61.6"
                     /tool="antismash"
                     /translation="ITDYAFGAILGREGLNLKAREMISVASLITLGNAQPQLELHMRAA
                     LNVGVTEKELLEIIIQMAVYAGIPACMNGLTAFR"
     CDS             8185..9363
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124:acetyl-CoA acetyltransferase (Score: 499.4;
                     E-value: 9.9e-152)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2362"
                     /transl_table=11
                     /translation="MRDVVIIAAKRTAIGGFQGSLANQPATTLGSIVIRDLLTESGITE
                     QDIDQVIMGQVLTAGCGQNPARQAALHAGLPVTIQAMTINKVCGSGLKAVHLGMQAIQN
                     GEAEIVIAGGQENMSLAPHILSMSRQGIRLGHSELKDSLIQDGLCDAFHQIHMGVTAEN
                     IAQKFDITRIQQDEYALMSHNRAINAQRAGYFKDEIVPVEIKGKKGETTWLMQDEGPKE
                     TSLEKLSTLKPVFQKKGSVTAGNSSSLNDGAAAVLLCSREKAKKLNLQPLAILGNFSNT
                     GIDPKIMGLAPIKAIHDCMLKQQWHPKDVDILESNEAFAAQSLAVISSCGISPERINPY
                     GGAIALGHAIGASGCRILVTLIHNLKRNQLNNGIAALCIGGGEGVALAITCP"
     PFAM_domain     8194..8970
                     /aSDomain="Thiolase_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00108.25"
                     /description="Thiolase, N-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2362_0878"
                     /evalue="1.20E-100"
                     /label="ctg1_2362"
                     /locus_tag="ctg1_2362"
                     /protein_end="262"
                     /protein_start="3"
                     /score="336.2"
                     /tool="antismash"
                     /translation="VVIIAAKRTAIGGFQGSLANQPATTLGSIVIRDLLTESGITEQDI
                     DQVIMGQVLTAGCGQNPARQAALHAGLPVTIQAMTINKVCGSGLKAVHLGMQAIQNGEA
                     EIVIAGGQENMSLAPHILSMSRQGIRLGHSELKDSLIQDGLCDAFHQIHMGVTAENIAQ
                     KFDITRIQQDEYALMSHNRAINAQRAGYFKDEIVPVEIKGKKGETTWLMQDEGPKETSL
                     EKLSTLKPVFQKKGSVTAGNSSSLNDGAAAVLLCSRE"
     aSDomain        8200..9351
                     /aSDomain="TIGR01930"
                     /aSTool="tigrfam"
                     /description="AcCoA-C-Actrans: acetyl-CoA
                     C-acyltransferase"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2362_0203"
                     /evalue="1.70E-137"
                     /identifier="TIGR01930"
                     /label="ctg1_2362"
                     /locus_tag="ctg1_2362"
                     /protein_end="389"
                     /protein_start="5"
                     /score="456.4"
                     /tool="antismash"
                     /translation="IIAAKRTAIGGFQGSLANQPATTLGSIVIRDLLTESGITEQDIDQ
                     VIMGQVLTAGCGQNPARQAALHAGLPVTIQAMTINKVCGSGLKAVHLGMQAIQNGEAEI
                     VIAGGQENMSLAPHILSMSRQGIRLGHSELKDSLIQDGLCDAFHQIHMGVTAENIAQKF
                     DITRIQQDEYALMSHNRAINAQRAGYFKDEIVPVEIKGKKGETTWLMQDEGPKETSLEK
                     LSTLKPVFQKKGSVTAGNSSSLNDGAAAVLLCSREKAKKLNLQPLAILGNFSNTGIDPK
                     IMGLAPIKAIHDCMLKQQWHPKDVDILESNEAFAAQSLAVISSCGISPERINPYGGAIA
                     LGHAIGASGCRILVTLIHNLKRNQLNNGIAALCIGGGEGVALAI"
     PFAM_domain     8992..9351
                     /aSDomain="Thiolase_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02803.20"
                     /description="Thiolase, C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2362_0879"
                     /evalue="2.40E-41"
                     /label="ctg1_2362"
                     /locus_tag="ctg1_2362"
                     /protein_end="389"
                     /protein_start="269"
                     /score="140.3"
                     /tool="antismash"
                     /translation="QPLAILGNFSNTGIDPKIMGLAPIKAIHDCMLKQQWHPKDVDILE
                     SNEAFAAQSLAVISSCGISPERINPYGGAIALGHAIGASGCRILVTLIHNLKRNQLNNG
                     IAALCIGGGEGVALAI"
ORIGIN
        1 gacaccgtgc tccgggaaga cccgggtatc aatgccgaaa acatcgcgta tttcgtcggc
       61 gcgcgccggg aaactgaccc gctttcgcaa aaaagcccta ttattctgga taatgcgggt
      121 tacccccggg cagaatgcgt gaaagatatt gcgtatgggc gtaatattga attgcattac
      181 ctgccgcctg acagcccaaa tctcagtccg atagagcgat tgtggaagta tatgaatgag
      241 ccagtgcgta acaatgtcta ttttccggat gcgcagacgt ttcgtgaaac cctgcgtcac
      301 tttttccatg tcatgttgcc agaaaaagcg aaagaactca cgacccgact gactgaccat
      361 tttcagattt taaaacctgc atcttcaggt tagattggta taggtctcaa taaactgtgg
      421 ctgtttatcg gcattaaatt tatgtggaac acccattgac ttcttgtagc tgaaaccctg
      481 acggtgaagc cattttgtca tgcctgagac agtgaaagtc acctgccatc gtgcccagac
      541 ataggccaca atttgtacgg tggtgtgcat cagattggct gtcagatatt caatcaactc
      601 agtggcttgt tcagccagta aacgactttc ggagccgccg ttttcaggcg cgagtttttc
      661 ttcggagaaa taatccttta gatgacgact caccgtactt tcagggatcc gcaaggcctg
      721 agcaatcctc tgagtagtcc atccttcgga agctaaaaga acggctttga tgcgatcaca
      781 aactcggcta tcgcgagttg tgtcatgcat caattcgagc gctttctttt ggtcttctgt
      841 cagatgaatt ttcatggtta gtaacattag ctaatgtgag tgaaaaatca agcatcttca
      901 atgacgagtg gtatacaacg cgctaaaaga aaaatgtggc cagaatgagc cgatattgtt
      961 tattgatgcg gtgcatccta cccagtcaac aaaattaagt tatggctgga tgaagagcgg
     1021 aagaaagcat gtcaaagtag ttgaaaccac gggcagtcgt actcggctta acatcatggg
     1081 cgccctgaat ttgcatcaaa ttgaaaacac gatcattcgt gaatatccga caattaatgc
     1141 aaaaaatgtc gtcctgtttt tcggtgcaat ccgggaaact tatccgctct cacaaagaat
     1201 tcatctcatt ctggatggag cgggttacca ccgttctgaa ttagttcaat tttttgccga
     1261 ggttctgaat atcgagttgc attatctgcc gccttatagc ccaaacctca atccaataga
     1321 gcgattgtgg aagtatatga atgagcaggt acacaacaat gtctattttc cggatgtaaa
     1381 aacgttccga gaaacacttc atcatttttt ccatgtcgta ttgcccaaaa aagcacaaga
     1441 actggctacc cggctgactg ataattttca gaccctaaaa cctgcatctt caagttaatt
     1501 gcgtatatta aggtatcttt gactttagtt gttgtttttt actttgtgtc tctaaatatt
     1561 agttatttat atagttatgc ggcaataaat attattccat gtttaattat tatcctttct
     1621 tttgcgcata atgatactgt gcagagaaaa accttcctct ctaaaaggtt ttttattttt
     1681 ctaggggaaa tttcattttc cttctatctt gtccatagaa tggttattat gtattcctct
     1741 gacggaatta gatatatcat tgatatgtat aatatgcatg ctatatcaat tatcatatta
     1801 tggttctcgt tatcaatatt gttgtcttat tttttatata aaataataga agttcctgtt
     1861 gttaatatgg ttcgtgaaat aatcaaaaag cagagtaaaa aacactcaag aggaaatagg
     1921 ttttatgaaa gagataagtt gtaatggttc aaccgttaaa tatagaattc atgctgctaa
     1981 aagcacagaa aatgctgaaa aaataatgtt cttgcatggg acaaatagtg atggaaacag
     2041 tagtttctct aatgtaaaaa atgaatttat agataaacta gatgtcgtta ttcctgatta
     2101 tgcaggctgt ggagaatcta cattaccatg ttcattggat gttgaaggat tagcagatca
     2161 aattatatct ataatagaaa attgtaattt tgataaacct atccatttag tgggtatttc
     2221 tttaggggct gttgttgctg ctgttgttgc tgctaagaga ccttcgtttg tcaataagtt
     2281 aattttaact gcaccttggg catctagcga tgatatccgt tttactttca tgtttaaaac
     2341 ttggtatgaa ttagaattaa aagataaacg aatggcaact gcgtttggtt tatctcatgt
     2401 tcttagccct aaaaaattgt cagatctttc aaatgaaaca attgatatga tatgtaataa
     2461 agaatcaaaa aacattgagg aaagaataaa aatcggactg aatataaata taactgaata
     2521 tttgaaggaa ataaagtctc ctacattagt cttaggatta tcttatgata atttaatacc
     2581 aacatatatg gttaaaaaag taagttcatt gatacctaac agtatatatg atgaaattga
     2641 aagcggacat gcggttcaaa ttgaaaatcc atcaatatgg atagataaga taaaagaatt
     2701 tatagaatat taaatccttt gatcctgagg gatttgtttt ctcgggatct attattttac
     2761 tacaaatttt ttgttatggg aatattataa acttatagtt tttatttttc cctttcattt
     2821 ttttatggtt tttaaaattt taaattgagt gaaaaataat aatgcgcttt gataacatct
     2881 ctataatagg taatggtgta tctggtattg ctacatttat ctccttagtt atgtacagca
     2941 atataaaaat aattactttt atatctccaa ctgaaatggg gggtcagaag cattctctga
     3001 tgctaaatag ctgttccaaa taatagatca cttgcaggga actcaatccg aattggacga
     3061 tctgattaat cgctaaacca aaaaaatcat ccctcggact gagttatgcc aatcatagca
     3121 tcgataccgc gcaatgagcg ccgtttaatg caaaaaacta tccagaaaac ccgcgataaa
     3181 aatcatgccc gaagacttat cgctatgctc atgttgtatc gcggagacac cgtcagctat
     3241 gtcgcgaaaa cactcgcgtg cagtcgttct tctgtcggtc gctggattaa caggtataca
     3301 ctttatggcc tggaaggctt aaaatcacgc cctcctggtc gtccacgacg ttggccattt
     3361 gaattaattt gttccttgct acgtcacctt atttcagatt ctcccgacgt attgggttat
     3421 caacgttcac gctggagcag tgagctactc gcgatacaaa tcaatgagat aaccggatgc
     3481 tcactccatg cctcaacgat acgccgatgg ctgccacaag cgaatattgt ctggcgccga
     3541 gcagtgccaa cattgcggat acgtgatccc cataaggaag agaaaatgag agcgattaat
     3601 gatgcgttgg agagatgcag ccccgatcat ccggtctttt acgaagatga agtggatatc
     3661 cacctgaatc ctaaaatcgg tgcagactgg caattacgtg ggcaacaaaa acgtatcacg
     3721 acaccgggtc agaacgagaa atattatctg gcaggggcct tacacagtgg caccggaaaa
     3781 gggagttatg ttggcggcaa caataaagac tcatcattat ttatcaaact attagcgcat
     3841 ctgaaagcag cctatcgccg ggcgaaaagt attaccttga tagccgataa ctacatcatt
     3901 cacaaaagcc agaaaacaca agagtggctc gccgaaaatc cgaaattcaa attgatttat
     3961 caaccgattt attccccatg gataaatcat attgaaaaat tatggttagc actccatgaa
     4021 acgattacac gtaatcattg ttgctgttat atgtggcagt tattgagaaa agtgcgtggc
     4081 tttatgaaca cggttagccc attcccagga aataaacatg gcacagcaaa agtgtagcaa
     4141 tattaggaac agctatttag ttttagttaa gaattaatat ttaaatataa agtataaatt
     4201 tatttataaa taaaaatcat aaaatgaggt atggaatatc ggatatagaa tacagactgt
     4261 gatatagaaa atatattaca aaaaaaatat ttccctatcc caaggtaaaa catttattat
     4321 taatcaagta atataaatca atgtaatgta ataaaaggat aaactataat gttctcaaaa
     4381 tatgtactta tcacgcgttt cccattgaaa gaaaaagtta atatgaaaaa actcaactct
     4441 ttagagatga acagtaataa cagatatttt atttcagaag aaaaagacat gaatgagtta
     4501 ttagaacttc gttccttttc gtcaattagt gaaatagaag aaatagaaaa tgaaatagaa
     4561 ttagacttca taaaattttc ttctatattg cgtggggata ttcgtcgaga gttagttaaa
     4621 tttgttgaag cccctattga ttctgaaaat gaacttccta tgacaaaata tatccaactc
     4681 cgtcatgtag aagtgccacc agaaaactat aatcggtacc gtcaatggcg ggatgaaact
     4741 atttttaacg tagtacgaga gaataaagat aaaattacca gcttcggtgc atatcactca
     4801 tttatcagtg gtcaacctgg tgttatgttc atttcatcat ttaatgtaaa caaagaaaaa
     4861 tacctagaat catttactaa tgaacattat caaaacatta taaaacaggc tggagacaac
     4921 tatatcaccg gaggaaacga tggtctttat acacgtattt atcgctctat acaagatcat
     4981 taaaaaataa ggttattaaa atgaaaaata ttacttcatg taaaaaaact atagttgtag
     5041 gagataagtt caatgagttt accaatggta aggatgcaat tactattagt cagctagacc
     5101 tcataacaca aattccagca catattatgg atactaataa acaatttgtt attggccaag
     5161 gagtaaggca tgattttgca gaaaaaatct tagaaaacaa aaagaataat aattataaca
     5221 ataaggaatt agatcttgaa aatatacaaa aatttgtttg ttacgagaaa aacaagtatt
     5281 ctcacaaaag aattccacac aatacaataa taggctccac tagaaaagaa aatgaaaact
     5341 cattctcatt accattatta attgatgaac ggtgtgagct tatgtctgat catcagacgg
     5401 gacagcatat ccaaggaatg attttaatag aagcctgtcg tcaaacattc attgctgtaa
     5461 cagaagaatt ttttatgtca gaaaaaatag gacagtctta ttatgttatc aatggaatgg
     5521 atatagaatt ctctaatttt ttattccctt tacctgcaat gataaactat aaagtaatag
     5581 ataaagatat taatgagaga cgttctagat ttaaagctga cattcatatt ttgcaacata
     5641 caaagcattg cgcttcgatg acggtttcat tcacagttta tcctaaaaaa gtgatcagtg
     5701 aaaaagaatc aatgatggcc gacgaaataa ctcaagccat tttgcgatcc cagcatataa
     5761 ctacacaaac ttttaaggaa aatgtgtatg cctgaaaaat acggtcagac atataatctg
     5821 gccgtcacta cacaagggcc catttaccct ccctctgaag ttgtagatgc ttatgggaat
     5881 tttgtagtga ttggtcaaat tgccactgat gaaggaattt attggggttg tgctatagta
     5941 gataaaaata gccctctacc accttatggg aatataaaat cataccatat tatacaaaat
     6001 ctgaatgact tatccgaaga gagtctagaa aaaattgttt tatatacact gccattacct
     6061 ctaccgatga acaattaccc tatgatattt gctccagaac aacgtccaaa tacagttcat
     6121 gataagcgct caagtaggcc attacacgaa ggatatatag cagattttag ggaggtagat
     6181 ggacggcgca aattacaacc tatcacgttg aaggactgga tgaaggctaa aggaacattg
     6241 acggtaactt tgagtaagga caaacgcagt gctagatttg attttacttt taatcatcta
     6301 ataccacata gtgtatatac tgtcatgagt ttacgtgaac gagatctagc accaaaaaat
     6361 cctagtaggc caggtcctct aggaattcct aatgttttta ttaccgatga caacggaaat
     6421 gcaacatatt gggcggaact acctaatcca ttccccgtaa agggggaaga acgcaaccga
     6481 gttattaata ttgttgttct tttcatgagt tgccaacaaa gttttggtgg tgctataggt
     6541 ttatatggtt tgggtggaga tattcatgct catctaaaat tacctgccgc ttctttcagt
     6601 gaattctata ctatggaata atgaaatgga gacatgaaaa ccattaattt ttattaaaaa
     6661 ataaaaaacc aataaggaat atataaacaa caatgagtaa gatagcggct tttttcgacg
     6721 ttgatgaaac cttaataaaa ataaaaagta tgtttcattt ttatcaattt tggtgttatc
     6781 aaaaaaaacg tcaagatgag tacaattcat ttaataaaca attcatcaat gccatttcta
     6841 aaggaactga aagagaagaa ttgaatagaa tgtattatcg acaactcaag ggagttcata
     6901 tagatgaatt atatcaggca ggtgaatatt ggttcaataa acatctgcaa aaagatgaaa
     6961 tttacattca atcagcaata aatgaatatt ttaatcataa gaagctagaa catttaacaa
     7021 tttttgtttc aggatcaatg ctccctttat taaaaccgtt aggaaaatat ttagatgtag
     7081 attttattct ttgcacaaaa ttattactag acaaagaaga tcgattaact ggagagattg
     7141 gtgaaccaca aactatagga aatggaaaaa aagaatcaat aataaaattc tcatataaaa
     7201 acaatattaa tttaaaaaat agctttgcct atggtgacga tatttccgat atacaaatgc
     7261 tcgagtcaac aggaaatcca atttgcattg gaaataacaa agaactaata aaacatgccg
     7321 aaataaacaa ttggaaaact attatttaac aatctaaatt agttaacaac caaaaatcag
     7381 caagaaatct tacattaaaa agagaagaat acttattata acatcattga aattatttat
     7441 ttcatgaagc atgaaaaggt attaaacata tacaccacga ctgatcaaaa atcaatcggt
     7501 gattactttt catattaaat tcacttttgc cagttgttat tttttaatat aaaaatcatt
     7561 ataataaact tacagaagtg aatttatcta atctcattag ataagttaca ttacacttta
     7621 aaatcaatga aattaaaaaa tcgcttatca aaacatgaag gatttttaat attgatgtta
     7681 ttacctgaga cattcttact tataaaaatt gtatcactat caaaatacat atagtttcat
     7741 cacccatcca tttacatgaa aaaaattagt aacgaatatg aaatctagta agacattaac
     7801 cgaacatggt cgctacatta tggagttatt agaacccggt ttagcagata aaataatgga
     7861 aaatttatct gaactggatt ctgatctacc atttttaatt accgactatg cctttggagc
     7921 aattttaggg cgtgaaggat taaatctaaa agcgagggaa atgatttcag tcgcatcgct
     7981 aattacttta ggcaatgctc aacctcaatt ggaattacac atgcgagctg cacttaatgt
     8041 cggagtgact gaaaaagaac tattggagat tataattcag atggcagtgt atgcaggtat
     8101 tcccgcttgt atgaatggac taactgcttt tcgagcagcg gtagctcaaa aaaatacact
     8161 aaataccaaa acgggagaga aattatgaga gatgtagtta tcatagcagc caaacgcaca
     8221 gcaatcggtg gctttcaagg ttcattagct aatcaacctg caaccaccct tggtagtatt
     8281 gttatccgtg atctactgac tgaaagtggc attacagaac aggacattga tcaggtcatt
     8341 atgggtcaag tgttaactgc tggttgtggt caaaatccag cgcgtcaggc tgcgctacat
     8401 gctggattac ctgtaactat tcaagctatg acaattaata aagtctgcgg ttcaggtctg
     8461 aaagctgttc atctgggtat gcaagcaatt caaaatggtg aagcagagat agttattgca
     8521 ggtggacaag aaaatatgtc tctcgctccc catattttat ccatgtctag gcaaggaatc
     8581 cgtcttggtc acagtgaact gaaagatagt ctgatacagg atggtctttg tgacgctttt
     8641 catcaaattc atatgggagt aacagcagaa aatattgcac aaaaattcga tataactcga
     8701 attcagcaag atgaatatgc acttatgtca cataatcgag ccattaatgc tcaacgggca
     8761 gggtatttca aggatgagat tgttcctgtt gaaataaaag gaaaaaaagg agagaccact
     8821 tggttaatgc aagacgaagg accaaaagaa actagtttag aaaagttgtc tactctaaag
     8881 cctgttttcc aaaaaaaagg gtcagtaaca gctggaaatt catcttctct caatgatggg
     8941 gctgctgcag tattattatg cagtagagaa aaagcaaaaa aactaaattt gcaaccatta
     9001 gctatattgg gtaatttttc taatactggt atcgacccaa aaatcatggg gttagctcct
     9061 atcaaagcaa tacatgattg tatgttaaaa caacaatggc atccaaaaga tgttgatata
     9121 ctcgaatcaa acgaagcatt tgctgcacaa tctctggctg taatttcatc atgtggtatt
     9181 tcgcctgaac gtattaatcc ctatggtggt gctatagctt tagggcatgc catcggtgcc
     9241 tcaggttgcc gtattcttgt aacattaatc cacaatctta agcgaaatca gttgaataac
     9301 ggtatagctg cattatgtat tggtggaggt gaaggagtag ctttagcaat tacttgtcca
     9361 taataattat ggtcaaagaa tttaaaatta tactgctaat gtacttggta ttattttata
     9421 tacaatacaa aattatgtta tttttataaa tactcaaata aggatatact taattgcact
     9481 tgacattata aaaatggatg gatatatcta attgctatat agcttttcat aatagtgaga
     9541 gtctttttaa atggtgagtt cttattcctc gctatacggg ggataaggat tactcctatc
     9601 attttgaaac tctatttagt aatataaatt atttccctta aattctggct gaacacatcg
     9661 tcgctgcttc atagtaaccc aaaatataca aataaaactc aaaacatcca aactattaag
     9721 cttagccaat aaaacagcct cctcagtcat ctcactcatt taataacata tttatactga
     9781 cgcgctgcgc ttgtcttctc tggtttgtct ggctaataca ttaaccagac aaatcagaga
     9841 gttgaaaaac acgatacccg ccagaaaagc cttttttatc taccccccta gtaaagaatt
     9901 agggatgaaa ctgttcaccg cattcaccat taattattta tatttaaatt cagtaagtta
     9961 aatggttaca acttaaagcc agcctcatca tcactcttca tataaactcc taccaaaatt
    10021 cagaaaaaag tgaatagagt gaagacttgt gaatagtaaa aaataactat tcacccaatg
    10081 agagcttgta atatctggct tcaatcaaag agtgaatagt gatgaacagt ttttcaggtt
    10141 ttccttagat aataaccgtg gagagaatta ttcttctggc tggtcttccc ttgttagctt
    10201 tggcatccat tcattggcag tttcgatatt caggctcata ttgctacgaa tgccttgttt
    10261 acttttctta cgaagataag gcaaaccata ttctgccaat gcacccggca tatccatacc
    10321 gaatcgggtc acggaaacag gcttgttcag gttgttacct ttcatatagg caaggtagga
    10381 gtgatagagg tatttacggg gattaaacgg cataatttcc gcattaccta tcaacatgcc
    10441 atcagcttca ttagacgcaa tcagataacc gcaaaaatcc actaaggaat ctgtgccacg
    10501 tttgatatct agcgcttctt cggatttttg ttgttcagcc agtaaacgct ttgcttcttt
    10561 cgggtcagca aatcgatgaa ataagtgctg gataattacg ggtaattccg ccgctatttt
    10621 gtctcgcaga aagggatcgc gttcatgttc cggcaccact tcggaaaatt tgaaaatcac
    10681 ccgacgccgc gatactccac cactgcgatc actgaaactc atggcgttat tgttcactgc
    10741 cagcaccaca gcaggaatac gagttgaata gggctgctta tgcttcgggt cgatg
//
