LOCUS       NZ_CP085627.1          10777 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP085627.1 Erwinia rhapontici strain BY21311 chromosome, complete
            genome.
ACCESSION   NZ_CP085627
VERSION     NZ_CP085627.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:41:16
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 4354940
            Orig. end    :: 4365717
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10777
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[4359940:4360717](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5777
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..10777
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10777
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     CDS             2077..2631
                     /locus_tag="ctg1_3980"
                     /transl_table=11
                     /translation="MTITLMRHGKPDHSLPGRRSSLAMAEWCEAYDLAEICDLPPERSL
                     RLAAEADWIVTSTLPRAISSLAKLGRQANRVDAVYREVALPVMPLAFPALSPLLWLPLL
                     RVMWLLGYAGQVESYAEAKQRAVLAADRLIALSAEGNVLLVGHGIMNKLIARRLRHLGW
                     LGEKHASSRYWSSAIYHPPVI"
     PFAM_domain     2230..2559
                     /aSDomain="His_Phos_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00300.24"
                     /description="Histidine phosphatase superfamily (branch 1)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3980_0177"
                     /evalue="9.00E-06"
                     /label="ctg1_3980"
                     /locus_tag="ctg1_3980"
                     /protein_end="161"
                     /protein_start="51"
                     /score="25.6"
                     /tool="antismash"
                     /translation="DWIVTSTLPRAISSLAKLGRQANRVDAVYREVALPVMPLAFPALS
                     PLLWLPLLRVMWLLGYAGQVESYAEAKQRAVLAADRLIALSAEGNVLLVGHGIMNKLIA
                     RRLRHL"
     CDS             2929..3063
                     /locus_tag="ctg1_3981"
                     /transl_table=11
                     /translation="MSSTCCITCYQHTEHRSLFDRLDKKVSERRDKEAGFVSLKQICL"
     CDS             complement(3261..4187)
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 145.9; E-value: 2.4e-44)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_3982"
                     /transl_table=11
                     /translation="MAIAEHLISSNLPSVKQLQCFLAVAHELNFRRAAERLRMTQPPLT
                     RQIKCLEAVLEIQLFSRSTHEVSLTEAGRELVIRAEKLLQQLSEMKTASPSSKKNLRIG
                     LTRTLNFELIEPINKQLNKLDAGDDVETPNLTSAQLLQCLSKNTLDLVLTGEKGTGHED
                     IVQYRWVYREPLLMAMPAMHPASLKEKVSLEDICDLPLFWFARSANPTFYDKCESYFDR
                     LRFPLKRIKEPDDSLVMLTHIARGKGFALMPQSKCTFNQEGLCYRELSDHESSKLNIDV
                     YAATRLNEKREDILNAQDVLCCNNLSE"
     PFAM_domain     complement(3330..3890)
                     /aSDomain="LysR_substrate"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03466.22"
                     /description="LysR substrate binding domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3982_0179"
                     /evalue="1.00E-15"
                     /label="ctg1_3982"
                     /locus_tag="ctg1_3982"
                     /protein_end="286"
                     /protein_start="99"
                     /score="57.8"
                     /tool="antismash"
                     /translation="NLRIGLTRTLNFELIEPINKQLNKLDAGDDVETPNLTSAQLLQCL
                     SKNTLDLVLTGEKGTGHEDIVQYRWVYREPLLMAMPAMHPASLKEKVSLEDICDLPLFW
                     FARSANPTFYDKCESYFDRLRFPLKRIKEPDDSLVMLTHIARGKGFALMPQSKCTFNQE
                     GLCYRELSDHESSKLNIDVYAATR"
     PFAM_domain     complement(3966..4142)
                     /aSDomain="HTH_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00126.29"
                     /description="Bacterial regulatory helix-turn-helix
                     protein, lysR family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3982_0178"
                     /evalue="1.00E-18"
                     /label="ctg1_3982"
                     /locus_tag="ctg1_3982"
                     /protein_end="74"
                     /protein_start="15"
                     /score="67.1"
                     /tool="antismash"
                     /translation="KQLQCFLAVAHELNFRRAAERLRMTQPPLTRQIKCLEAVLEIQLF
                     SRSTHEVSLTEAGR"
     CDS             4379..4990
                     /locus_tag="ctg1_3983"
                     /transl_table=11
                     /translation="MSSNYVLVSRFPLKNTVSADTFSSLKNTESTRYYFCEEQGVNELL
                     ELRSFNDIKEVSWVEEEMESMFHQFADVLSADIRRELLKFVESPIDSKRDLPETKYIQL
                     RHVEVPAHNYQQYRQWRDETIFNVVRDNKDKIESFEAFHSLISGVPGVMFISAFNGDKE
                     TYKEAFTNARYQEIIQQAGDNYITGGNEGLYTRIYRACCC"
     CDS             5001..5777
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_3984"
                     /sec_met_domain="AfsA (E-value: 7.3e-10, bitscore: 36.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKKLVVVGDKFNEFANGKDVLTISQLELLTQIPANIIDKEHEI
                     IIGQGVRKDFAGKVLSNQARNTIHGNKLKMCSLEKLVNDKKNEHCHKRLEQNVLIGPAE
                     QTEHNSNLFAMPLLIDERCELMADHQTGQHIQGMLLVEASRQAFIAITEEFIYKQETGR
                     YYVINSMAINFSSFLFPLPALVHFEYLEKDVNDRRGRFKAQVRVTQHQMLCASMDVSFT
                     VYPSGLISEKEKSLAEAAMQAAIVEHQGATFEVAHA"
     PFAM_domain     5271..5666
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3984_0180"
                     /evalue="1.20E-13"
                     /label="ctg1_3984"
                     /locus_tag="ctg1_3984"
                     /protein_end="222"
                     /protein_start="90"
                     /score="51.3"
                     /tool="antismash"
                     /translation="HKRLEQNVLIGPAEQTEHNSNLFAMPLLIDERCELMADHQTGQHI
                     QGMLLVEASRQAFIAITEEFIYKQETGRYYVINSMAINFSSFLFPLPALVHFEYLEKDV
                     NDRRGRFKAQVRVTQHQMLCASMDVSFT"
     CDS             5770..6603
                     /locus_tag="ctg1_3985"
                     /transl_table=11
                     /translation="MPEISSQKYRLTATTQGPLYPPAEVMDQDGNFVVVGMVPGDNGLA
                     WRSMIVAADSPLPGFGETAPYKILRDIDDMSQAELKDVILHTLPLPVPLNNYGMLFAPE
                     QRPQANTESRPSLPLHEGYIADYRSSDGKRQIPPVTLADWLRAEGSLEVFLSGDSRRAL
                     FRFSFRSLVPDSVYTVMSLREQDLATENPSRPGPLGIPNVFITDHDGNADYWAELPDPF
                     PAHERNDNRVINVVVLYMSSRQSYGGAIGLYGLGGDIHAHLKLKSRSFDELITVK"
     CDS             6611..6835
                     /locus_tag="ctg1_3986"
                     /transl_table=11
                     /translation="MPFVHITTWPAKDEGDMLRLHEDITYAVHKNTGAPLDKISVVISE
                     IQPSRWSDAGIPGDHKDFPVKSRRKSYEE"
     PFAM_domain     6614..6763
                     /aSDomain="Tautomerase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01361.23"
                     /description="Tautomerase enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3986_0181"
                     /evalue="7.50E-06"
                     /label="ctg1_3986"
                     /locus_tag="ctg1_3986"
                     /protein_end="51"
                     /protein_start="1"
                     /score="25.9"
                     /tool="antismash"
                     /translation="PFVHITTWPAKDEGDMLRLHEDITYAVHKNTGAPLDKISVVISEI
                     QPSRW"
     CDS             6839..7489
                     /locus_tag="ctg1_3987"
                     /transl_table=11
                     /translation="LQKSAAFFDVDETLITIKSMFDFYAFWCRENNEMNTLECYMTQFK
                     ENVQKGTSREYLNREYYRQFSGVSYKKLEEVGEKWFRSQCVKTLFINSAVAALKQHQAE
                     NRLTIFVSGSMRPVLAPVARYLGVTEILCAPLELTEAGTLTGEIGTPQTIGSGKREALI
                     AFCRGKEISPADCYAYGDDLSDIPMLESVGHPICVGKKSSLASHAIHQHWPVI"
     aSDomain        6851..7414
                     /aSDomain="TIGR01488"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB: HAD phosphoserine phosphatase-like
                     hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_3987_0035"
                     /evalue="1.10E-24"
                     /identifier="TIGR01488"
                     /label="ctg1_3987"
                     /locus_tag="ctg1_3987"
                     /protein_end="192"
                     /protein_start="4"
                     /score="85.4"
                     /tool="antismash"
                     /translation="AAFFDVDETLITIKSMFDFYAFWCRENNEMNTLECYMTQFKENVQ
                     KGTSREYLNREYYRQFSGVSYKKLEEVGEKWFRSQCVKTLFINSAVAALKQHQAENRLT
                     IFVSGSMRPVLAPVARYLGVTEILCAPLELTEAGTLTGEIGTPQTIGSGKREALIAFCR
                     GKEISPADCYAYGDDLSDIPMLESV"
     aSDomain        6851..7438
                     /aSDomain="TIGR01490"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB-hyp1: HAD hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_3987_0034"
                     /evalue="1.60E-39"
                     /identifier="TIGR01490"
                     /label="ctg1_3987"
                     /locus_tag="ctg1_3987"
                     /protein_end="200"
                     /protein_start="4"
                     /score="134.2"
                     /tool="antismash"
                     /translation="AAFFDVDETLITIKSMFDFYAFWCRENNEMNTLECYMTQFKENVQ
                     KGTSREYLNREYYRQFSGVSYKKLEEVGEKWFRSQCVKTLFINSAVAALKQHQAENRLT
                     IFVSGSMRPVLAPVARYLGVTEILCAPLELTEAGTLTGEIGTPQTIGSGKREALIAFCR
                     GKEISPADCYAYGDDLSDIPMLESVGHPICVGK"
     PFAM_domain     6854..7408
                     /aSDomain="HAD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12710.9"
                     /description="haloacid dehalogenase-like hydrolase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3987_0182"
                     /evalue="9.00E-25"
                     /label="ctg1_3987"
                     /locus_tag="ctg1_3987"
                     /protein_end="190"
                     /protein_start="5"
                     /score="88.3"
                     /tool="antismash"
                     /translation="AFFDVDETLITIKSMFDFYAFWCRENNEMNTLECYMTQFKENVQK
                     GTSREYLNREYYRQFSGVSYKKLEEVGEKWFRSQCVKTLFINSAVAALKQHQAENRLTI
                     FVSGSMRPVLAPVARYLGVTEILCAPLELTEAGTLTGEIGTPQTIGSGKREALIAFCRG
                     KEISPADCYAYGDDLSDIPMLE"
     CDS             7512..8726
                     /gene_functions="transport (smcogs) SMCOG1184:major
                     facilitator transporter (Score: 292.6; E-value: 5.7e-89)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_3988"
                     /transl_table=11
                     /translation="MNVESTPQDMTLRSWLALFILALSTFTIVTTELAPVGLLTPIANG
                     LHASESAVGMTVSLYAWVGALSALFASVFLGNVAKKRLLLTLTVILFLSNVLAATVSTY
                     TVLLAARVLGALAHGAFWAMIGATAVSIVPTRYIGAATSIVFGGVSAASVFGVPVSNYI
                     GIHFGWRQAFWLMAILSVVAFAGISVLIPQITSKSAIGFEALKSVLKSSALWKIYSATL
                     LAVTAHFAAFTYIEPWLHTQHSLATSVIPAVLFVYGIAGLAGNFLTGMMIDKYLKATVS
                     ISVLLICAVLVFLGTSGSALSGTAVFTAMVIWGVAVSGIFVGFQTWVLRLAEDKTFPAS
                     AVYVSFFNTAIGIGASAGAWMVSAFSVPMLYVVAGVAIGLSVLLVAAIPTRVASAQNVL
                     EKSYE"
     PFAM_domain     7557..8507
                     /aSDomain="MFS_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07690.18"
                     /description="Major Facilitator Superfamily"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3988_0183"
                     /evalue="3.70E-30"
                     /label="ctg1_3988"
                     /locus_tag="ctg1_3988"
                     /protein_end="332"
                     /protein_start="15"
                     /score="105.1"
                     /tool="antismash"
                     /translation="LALFILALSTFTIVTTELAPVGLLTPIANGLHASESAVGMTVSLY
                     AWVGALSALFASVFLGNVAKKRLLLTLTVILFLSNVLAATVSTYTVLLAARVLGALAHG
                     AFWAMIGATAVSIVPTRYIGAATSIVFGGVSAASVFGVPVSNYIGIHFGWRQAFWLMAI
                     LSVVAFAGISVLIPQITSKSAIGFEALKSVLKSSALWKIYSATLLAVTAHFAAFTYIEP
                     WLHTQHSLATSVIPAVLFVYGIAGLAGNFLTGMMIDKYLKATVSISVLLICAVLVFLGT
                     SGSALSGTAVFTAMVIWGVAVSGIFVGFQTWVLRLA"
     CDS             8719..9132
                     /locus_tag="ctg1_3989"
                     /transl_table=11
                     /translation="MSEASDLTRYGKDIMDRLEPGLADKVTGRLAELDPSLPALITDYA
                     FGAVVGRPGLDLKTREMLTVASLVTLGNAMPQLELHMRAALNTGVTPEELLEIVIQMAV
                     YAGVPACMNGLTAYRAAMAATGHTLPGLKGKSA"
     PFAM_domain     8821..9069
                     /aSDomain="CMD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02627.22"
                     /description="Carboxymuconolactone decarboxylase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3989_0184"
                     /evalue="4.30E-19"
                     /label="ctg1_3989"
                     /locus_tag="ctg1_3989"
                     /protein_end="117"
                     /protein_start="34"
                     /score="68.5"
                     /tool="antismash"
                     /translation="PSLPALITDYAFGAVVGRPGLDLKTREMLTVASLVTLGNAMPQLE
                     LHMRAALNTGVTPEELLEIVIQMAVYAGVPACMNGLTA"
     CDS             9129..10313
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124:acetyl-CoA acetyltransferase (Score: 484.5;
                     E-value: 3.2e-147)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_3990"
                     /transl_table=11
                     /translation="MNEVVIVSARRTATGAFMGVLSGHSAVALGSHVIRALLEESGVKA
                     SEISQVIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSVHLGMQALLT
                     GEATFVIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDTHMGVTAEN
                     VADEFGITREMQDEFALLSHMKAIRAQEQHLFEREIVPVEWQNRKGETQRVDTDEGPRQ
                     TSIEKLDKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTRRTAEAQGLPVLASLGEFTNS
                     GIKPALMGAAPIQAITDCLTRSGWKPEDIEHLESNEAFAAQALAVIAQTGISQERINPY
                     GGAIALGHAIGSSGCRILVTLVHGLIRTNSQRGMAALCVGGGEGVALSVYRQKS"
     PFAM_domain     9138..9911
                     /aSDomain="Thiolase_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00108.25"
                     /description="Thiolase, N-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3990_0185"
                     /evalue="1.40E-98"
                     /label="ctg1_3990"
                     /locus_tag="ctg1_3990"
                     /protein_end="261"
                     /protein_start="3"
                     /score="329.5"
                     /tool="antismash"
                     /translation="VVIVSARRTATGAFMGVLSGHSAVALGSHVIRALLEESGVKASEI
                     SQVIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSVHLGMQALLTGEA
                     TFVIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDTHMGVTAENVAD
                     EFGITREMQDEFALLSHMKAIRAQEQHLFEREIVPVEWQNRKGETQRVDTDEGPRQTSI
                     EKLDKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTR"
     aSDomain        9144..10292
                     /aSDomain="TIGR01930"
                     /aSTool="tigrfam"
                     /description="AcCoA-C-Actrans: acetyl-CoA
                     C-acyltransferase"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_3990_0036"
                     /evalue="1.10E-128"
                     /identifier="TIGR01930"
                     /label="ctg1_3990"
                     /locus_tag="ctg1_3990"
                     /protein_end="388"
                     /protein_start="5"
                     /score="427.4"
                     /tool="antismash"
                     /translation="IVSARRTATGAFMGVLSGHSAVALGSHVIRALLEESGVKASEISQ
                     VIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSVHLGMQALLTGEATF
                     VIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDTHMGVTAENVADEF
                     GITREMQDEFALLSHMKAIRAQEQHLFEREIVPVEWQNRKGETQRVDTDEGPRQTSIEK
                     LDKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTRRTAEAQGLPVLASLGEFTNSGIKPA
                     LMGAAPIQAITDCLTRSGWKPEDIEHLESNEAFAAQALAVIAQTGISQERINPYGGAIA
                     LGHAIGSSGCRILVTLVHGLIRTNSQRGMAALCVGGGEGVALS"
     PFAM_domain     9936..10295
                     /aSDomain="Thiolase_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02803.20"
                     /description="Thiolase, C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_3990_0186"
                     /evalue="5.80E-39"
                     /label="ctg1_3990"
                     /locus_tag="ctg1_3990"
                     /protein_end="389"
                     /protein_start="269"
                     /score="132.6"
                     /tool="antismash"
                     /translation="PVLASLGEFTNSGIKPALMGAAPIQAITDCLTRSGWKPEDIEHLE
                     SNEAFAAQALAVIAQTGISQERINPYGGAIALGHAIGSSGCRILVTLVHGLIRTNSQRG
                     MAALCVGGGEGVALSV"
ORIGIN
        1 gatacctgcc atcaccgcaa aataagtggc aatcggccac cagtgacccg tccaggccag
       61 cagtgccgac gctaccagcg gtgccgtacc acctgaaaga attgacccca gctctttagc
      121 cgtggccatt ttggtataac gatgatgaac gccaaacatc tccacgcccc aggccgcctg
      181 tacgccgaag atccccagcg atgccaatcc catcccgacg acgatcgtac tgatgacaat
      241 ggccggttca cgcgagtcca gcagcataaa ggccgggaac gcgtagagga tcagcagcag
      301 gcagaagcag cgataggtaa tacggcgacc aaagcggtcc gacaaccagc ccgccagcgg
      361 gataatcagg aagcccagca gcgaagcaat aaacaccgcc gtggtcgcca ccgatttatc
      421 cactgccagc actttcacca cataaccgat aataaagccc tgcgccagat aggatggacc
      481 gttctcaccg atacgcagac caaccatggt caggaaggca cggctacggc gccagaatcc
      541 acgggtatcc tctacgacgg gcgcggcggc cagcacctgc tcacgttcgg cttccagctc
      601 tgcttttttg cgttcaaata ccggggtttc acgtaagtga cgacggatcc ataacgccac
      661 cagcgcaatc agcgaactgc acaggaacgg aatacgccag ccccactctt gcagtgattg
      721 ttggtccatc tgcaccaccg ccagccagac caacgatgcc agcagcgtgc cgctgttaga
      781 accgagcgca ataatggaag agaccagacc gcgacgctgc gcgggtgcgt attcacccag
      841 cattaccgta ccgccggaca gctctgcacc cgcaccaaag ccctgactaa agcgtagcag
      901 caccagacac gccggtgccc aaaggccaat agaggcatag ctggggataa gaccaatcag
      961 tgtggtggag gcacccatca ggatgatggt ggaaaccata acgacttttc gacctttacg
     1021 atcgccgagc cagccaaaga acagtgcgcc aatcggacgg gcaataaagc caaccgacca
     1081 ggtcgcgaag gtagagagga gtgccatttc tggcgttgct tcggggaaga agacatcacc
     1141 gaagataatc ccggcggcca gcccatataa agcgaagtcg gcatattcca ttgctgtgcc
     1201 aagccagcac gagaatgttg cccgccaaaa atccttacgc ccttccgggg tggctaaacg
     1261 ttcatctgcc gccgagttgg ccgacattgc agtatcctgt gcaacgcttt gaggtgtaga
     1321 catgaaaaat tccctgatgt gaggagtttt attcggcgcc atccctggca cgcactcctc
     1381 cgtcggagga aaattcgcca gtgaacaggc cgtgttataa agccatgacg aaaggtcgtt
     1441 taccctaaat aattcgagct gcaggaaggc ggcaaaggag cgaaccccga tgagcttact
     1501 gaagtaagtg attcgggtga acgactgcag ccaacgcaca tgcaacttga agtatgacgg
     1561 gtaaaaaggt tccctggtgt tggaacgcta tattcgcttt gcgccgacgg tctaaaattg
     1621 accaatcgta taagtaacgc gaaacaaatt tgaccttcac gtagctgcta accatcaagc
     1681 accgtgaatt ttcactacgc agctattgta tctacgcgtg tagataaatc aaggtaacga
     1741 aacggcaatc gttagatttt cttgacattc aacacaactg attgcttaga aaatcaataa
     1801 aatcaatgaa ataaatcgtc caaaaaacat gcgatttttt ttcactattg aaaccgtgcg
     1861 caaacttaag gcaaatgaag ctgaacacgc tttcaacact gacgttttag cccccgagta
     1921 ttgcgaagag atgacaatct gcacattagc ttcaggtttg tgtttgtcgg gggggatggt
     1981 taagctgaaa aacacactta gagcctggcc cagtaggcgt acattaatgg caaataaaat
     2041 agcctaatgg gccaggcact tatcaggcgg gaaataatga cgattacatt aatgcgccac
     2101 ggcaagcccg atcactctct gccaggccgc cgctcctcac tggcgatggc ggaatggtgc
     2161 gaggcctacg atctggccga gatctgtgac ctgccgccag aacgcagcct gaggctggcc
     2221 gcagaggcgg attggattgt caccagcaca ttaccgcgtg ccatttcgtc gctggcaaaa
     2281 ctcggcaggc aggccaaccg ggtcgatgca gtctatcgtg aagtggcact gcccgttatg
     2341 ccgctggcct tcccggcttt atcgccgctg ctgtggctgc cgctgctgcg ggtgatgtgg
     2401 ctgctgggct acgccggaca ggtagagtct tacgctgagg caaaacagcg tgcggtgctc
     2461 gccgccgatc gcctgatcgc cctgtctgcc gaaggcaatg tactgctggt gggtcacggc
     2521 attatgaata agctgattgc acgccgctta cgtcatcttg gctggctggg agagaaacac
     2581 gccagcagcc gctactggag cagcgccatt tatcatccac cggtgattta gcgctaaagg
     2641 cgcatgagag caggtctgga gagataatgg ttatgattca accagttcgc gatatcgtat
     2701 ttttgcctgg aaatgcatca tacacatgtt aagcgctgga tcgtgccgca gagttacatt
     2761 ataatcctgc gcctcaggcc ccatagctca gttggttaga gcacgcgact cataatcgct
     2821 tggtcgctgg ttcaagtcca gctggggcca ccaaataaaa caaggactta gacaggaaac
     2881 tggctgagtc ctttttgttt tctggcgtta caggtgaggt agtgggtgat gtcatccaca
     2941 tgctgcatca cctgctatca gcacacagaa cataggagcc tgtttgacag actcgacaag
     3001 aaagtgagtg agcggcgtga caaagaagcc ggcttcgtca gccttaaaca aatttgcttg
     3061 tagtatgaga tctgtcaggc catcagcgtc atggcaagat cttgccgcgc ttatcaacgt
     3121 taattagaaa cgggcaatac ttatcttcgt tatgcaacgg gtgggcatct gtggtctgtg
     3181 cttcgctgtt gtgtgccctg attttcatca ttagtggggc gaagcctggc gtgaagaaag
     3241 ttttgactat ataccccaat ctactctgac agattgttac aacataacac gtcctgcgca
     3301 ttcaggatgt cttctcgctt ttcattgagt cgggtagccg cgtagacatc gatattcagc
     3361 tttgacgatt cgtgatcgga cagctcccgg tagcatagcc cctcctgatt gaacgtgcac
     3421 tttgactgcg gcatcaaggc aaaccccttg ccccgggcga tgtgggtgag cataaccagt
     3481 gagtcatctg gctctttgat ccgcttaagc ggaaacctga gccgatcgaa gtagctttca
     3541 cacttatcgt agaaggtggg attggcgctt cttgcaaacc agaagagcgg taaatcacag
     3601 atgtcttcca gcgagacttt ttctttcagg cttgcaggat gcatagccgg cattgccatt
     3661 aacagcggct cgcggtagac ccaacggtac tggacgatgt cttcatgacc agtgcctttc
     3721 tcgccggtga gcacaagatc aagcgtgttt tttgacagac attgaagcag ctgcgccgat
     3781 gtcagattgg gagtttcaac atcatcgccg gcatccagtt tattgagctg tttgttgata
     3841 ggctctatca gctcaaaatt cagcgtacgg gtgagaccga tacgcagatt cttctttgag
     3901 gaaggtgaag ccgttttcat ttcgctgagc tgctgaagga gcttctctgc cctgataacc
     3961 agttcgcgac cagcctctgt cagactgacc tcgtgcgtgc tgcggctgaa cagctgtatc
     4021 tccagcacag cttccaggca ctttatctga cgggtcagag gaggttgggt catcctgaga
     4081 cgttctgcgg ctctgcggaa gttcagctca tgtgcgacag cgagaaagca ctgaagctgc
     4141 ttaacgctgg ggaggttgct actgattaaa tgttctgcaa ttgccataga ttccatcctt
     4201 caaatagaaa aattattcta tcttatttat acgtaaagat ataacacgga atttcatttc
     4261 agaaaaaggc tcttttacag taatacaaaa tttatattac acatttaaca attccacctg
     4321 cgtcacagat tgataaatta cactcgaaat ctaatcccat atcctttcgg agataataat
     4381 gtcatctaac tatgtgctgg tgtctcgatt cccattaaaa aatactgttt ctgctgatac
     4441 tttctcgtca ttaaaaaaca ctgagagcac gcgctattat ttttgtgaag aacagggtgt
     4501 aaatgaatta ctggaacttc gttcgtttaa cgacattaaa gaagtatcat gggtggaaga
     4561 agagatggaa tctatgttcc atcagtttgc tgacgttctt tccgcggata ttcgcagaga
     4621 acttctgaag tttgtcgagt cacctatcga cagcaagaga gatctccctg agacaaaata
     4681 tattcagctg cgccatgttg aagttcctgc acacaattat cagcagtatc gccagtggcg
     4741 cgacgaaacg atcttcaacg ttgtaagaga taataaagac aagatcgaaa gctttgaagc
     4801 ttttcactcg ctcatcagtg gcgtgccagg tgtgatgttc atttctgcgt tcaatggcga
     4861 taaagaaacg tataaagaag cctttaccaa tgcacgctac caggaaatca ttcagcaggc
     4921 aggtgataac tatattaccg gaggaaatga aggtctctat acccgtatct atcgcgcttg
     4981 ttgttgctga ggaatgattc atgaattgta agaaactggt tgttgtcggc gataaattca
     5041 atgaattcgc aaatggaaaa gatgtcctga ctatttctca gctggagctg ttaacgcaaa
     5101 tccctgcaaa tatcatcgat aaagaacatg aaattattat cgggcagggt gtacgtaagg
     5161 attttgccgg gaaagtgctt tcaaatcagg cgagaaatac cattcacggc aataaactaa
     5221 aaatgtgttc tcttgaaaaa cttgttaacg ataaaaagaa tgaacactgc cataaacggc
     5281 tggagcagaa tgtcctgata ggtcctgcag aacagactga gcacaacagt aatctttttg
     5341 ctatgccgct gttgatcgat gagcgctgtg agctgatggc cgatcatcag accggtcagc
     5401 atattcaggg aatgctcctg gtagaggcaa gccgtcaggc gtttatcgcc attactgaag
     5461 aatttatcta caagcaggaa acggggcgtt actacgtcat taacagcatg gcgataaact
     5521 tttcgagttt tctgtttcct ttacctgcgc tggttcattt tgagtacctt gagaaggacg
     5581 ttaatgaccg ccgtggccgt ttcaaagccc aggtgcgcgt aacgcagcat cagatgcttt
     5641 gcgccagcat ggacgtctca ttcaccgtat acccttcagg gctgatttct gagaaagaga
     5701 aatccctggc tgaggcggct atgcaggcag cgatagtaga acatcagggt gcgacctttg
     5761 aggtagcgca tgcctgaaat aagcagtcag aaataccgtc tgactgctac cacgcagggg
     5821 ccgctttatc cccctgcgga ggtaatggat caggatggta actttgtcgt ggtcggtatg
     5881 gtgccaggag acaacggcct ggcatggcgc agcatgattg tggcggcaga cagcccgctt
     5941 ccgggctttg gagaaacggc accctacaaa atattgcgtg atatcgatga catgtcacag
     6001 gcagaactga aggacgtgat cctgcatacc cttccgctgc cggtaccgct gaataattac
     6061 gggatgctgt tcgcgccaga gcagcggcca caggctaata ccgaaagccg gcctagcctg
     6121 cctttgcatg aaggctacat cgcagattac cgcagcagtg acggtaaacg tcagatcccg
     6181 cctgtcacgc tggcagactg gttacgcgct gaaggcagtc ttgaggtgtt tctgtcagga
     6241 gacagccggc gcgcgctgtt caggttctca tttcgcagcc tggttcccga cagtgtttat
     6301 accgtaatga gcctgcgtga gcaggatctg gcaactgaaa atccaagccg gccaggaccg
     6361 ctaggtatcc ctaacgtatt tattaccgac cacgatggca atgcggatta ctgggcggag
     6421 ttacccgatc cgttcccggc acatgaacgc aatgataatc gggtaattaa cgtggttgtc
     6481 ctttatatga gttcccgcca gagctacggg ggcgcaattg ggctgtatgg gctgggtggt
     6541 gatatccatg cacatctgaa actgaaaagc cgctcattcg atgaattaat taccgtaaag
     6601 tgagttaaca atgccatttg tacatatcac gacctggcct gctaaagacg aaggcgacat
     6661 gctcaggctg catgaagaca ttacctatgc cgtgcataaa aacaccggag caccgctgga
     6721 taaaatctct gtagtgattt ctgaaataca gccctcacgc tggtctgatg caggtattcc
     6781 cggggatcat aaagatttcc cggtaaaaag ccgaagaaag agttacgagg aataagcttt
     6841 gcagaaatca gccgcatttt ttgatgtcga cgagaccctt ataacaatta aaagcatgtt
     6901 tgatttttac gcattctggt gtcgtgaaaa taatgaaatg aacacgcttg aatgctatat
     6961 gacgcagttt aaggaaaacg ttcaaaaagg aacgagtcgc gagtatctga acagagagta
     7021 ttaccgacag ttctctgggg tcagttataa aaaactggaa gaggtgggtg aaaaatggtt
     7081 ccgatcacag tgtgtcaaaa ctctctttat taacagtgcc gtcgcagccc ttaagcagca
     7141 tcaggcagaa aacagactga ctatctttgt ttcaggatcg atgcgtcctg tacttgcacc
     7201 ggttgcccga tatctgggtg tcacggagat tctctgtgcc ccactggaac tcactgaggc
     7261 aggaacactg acaggtgaaa taggcacgcc gcagaccatt ggcagtggca aacgggaggc
     7321 acttattgcg ttctgtcgcg gaaaagaaat cagtcctgcg gattgctatg cctatggaga
     7381 cgatctctcc gatattccca tgcttgaatc cgtcggccat ccgatttgcg taggaaagaa
     7441 gtcatcgctt gccagtcatg cgattcacca acactggccc gttatttaaa tcattccaga
     7501 ggtaaaaaat tatgaacgta gaaagcactc cgcaggatat gaccttaagg tcgtggcttg
     7561 ctctttttat tctggctctc tcaacgttta cgatcgtcac cacagagctg gcacctgttg
     7621 gcctgctgac gccaatcgca aacggcctgc atgcgtctga atcagccgtg ggtatgacgg
     7681 tctcactgta tgcatgggta ggtgcactga gcgcactttt tgcatcagtg tttctgggca
     7741 atgtggccaa gaagcggctg ctgcttacgc tcaccgtgat ccttttcctg tctaacgtac
     7801 ttgccgcgac ggtgagcact tacacggtac tactggctgc acgtgtgctg ggagcgctgg
     7861 cacacggtgc attctgggca atgattgggg caacggccgt ttccattgtg ccgacaagat
     7921 atattggcgc cgctacctca atcgtatttg gtggcgtatc tgcagccagt gtgtttggcg
     7981 tgccggtttc aaactatatc ggcattcact tcggctggcg gcaggcgttc tggctgatgg
     8041 cgatactcag cgtggtggca tttgccggta tcagcgtgct gatcccacag atcaccagca
     8101 aaagcgcaat tggctttgag gcgctcaaaa gcgtactgaa atcatctgca ctgtggaaaa
     8161 tctattctgc tacgctgctc gctgtcaccg cgcactttgc tgcctttacg tacattgaac
     8221 cctggctgca cacgcagcat tcactggcca catcggttat tccggctgtg ctgtttgtat
     8281 acggtattgc gggacttgcc ggcaatttcc tgaccggcat gatgattgat aaatatctca
     8341 aggcgacagt atccatttca gtactgctca tttgtgcggt actggtattc ctgggaacat
     8401 ccggttcagc gctttcaggc actgctgtct tcaccgctat ggttatctgg ggcgtggcag
     8461 tatccggaat ctttgtgggc ttccagacgt gggtcctgcg cctggctgaa gataaaacct
     8521 ttcctgcctc tgcggtttac gtttcatttt tcaacactgc tatcggtatc ggtgcctctg
     8581 ccggtgcgtg gatggtatcc gcattttcag ttccgatgct ctacgtcgta gctggcgtgg
     8641 ccatcgggct ctctgtactg ctggtggcgg ctatcccgac gcgcgtggcg tctgcacaaa
     8701 acgttctgga gaaatcttat gagtgaagct agcgatctga cccgttatgg caaagacatc
     8761 atggaccgcc ttgaacccgg tcttgccgat aaagtcaccg gacgactggc tgaacttgat
     8821 ccttcattac cggccctgat caccgactac gcatttggtg ctgtggtggg gcgccccgga
     8881 ctggacctca agacccgtga aatgctgaca gtggcatcac ttgtcacgct gggtaatgcg
     8941 atgccgcagc tggagctgca tatgagagcg gctctcaata ccggcgtcac gcccgaggag
     9001 ttgcttgaaa tcgtaattca gatggcggtt tatgcaggcg tcccggcttg tatgaatggc
     9061 ctgactgcct atcgtgccgc aatggcggca accgggcata cattacccgg tctgaaagga
     9121 aaaagtgcat gaatgaagta gtgattgttt ctgcaagacg cacggcaacc ggtgcattta
     9181 tgggtgtact gtccgggcac tccgccgtcg cgctgggaag tcatgttata cgcgcgcttc
     9241 ttgaagagtc cggcgtcaaa gccagtgaga tttcacaggt cattatggga caggttctga
     9301 ccgctggctg cggtcagaac cccgcacgcc agtccgcact gaaagccgga ctgcctgtcg
     9361 aaacacagtg tatgaccatc aacaaagtct gcggttcggg cctgaaatcg gttcatctgg
     9421 gtatgcaggc actgctcacc ggagaggcaa cgtttgtgat tgctggtggt caggaaagca
     9481 tgtccagtgc accgcatatc ctgacatcgt cgcgtaccgg gacacgtctc ggaaacagcg
     9541 agatgaaaga cagtctggta tctgatgggc tgtgggatgc ttttgatgat acccacatgg
     9601 gcgttactgc tgaaaacgtg gcggatgagt ttggtatcac tcgtgaaatg caggacgaat
     9661 tcgcgctttt atctcacatg aaggcaatca gggcgcagga gcagcacctc tttgaacgcg
     9721 aaattgttcc tgtagagtgg cagaacagaa agggtgaaac gcagagggtt gatactgatg
     9781 aaggtccgcg tcagaccagc attgaaaagc tcgataagct caaacctgta ttcagaaagg
     9841 ggggcacagt aactgctggt aatgcctcct cacttaacga cggtgccgcc gctgttcttc
     9901 tgtgcacccg gcgtacggct gaggcacagg gattgccggt gctggcttct ctgggagagt
     9961 tcaccaactc aggtatcaag ccggcactaa tgggtgccgc tccgatacag gcaatcacag
    10021 actgccttac ccgttcaggc tggaagccag aagacattga acatctcgaa tcaaatgagg
    10081 cgtttgcggc gcaggctctc gcagttatcg ctcagacagg catttcacaa gagcgcatca
    10141 acccttacgg tggtgctatc gcgcttggtc atgctattgg atcgtcgggg tgccgtatac
    10201 tggtcacgct tgtacacggc ctgatacgaa ccaacagcca acgcggtatg gctgcactgt
    10261 gtgttggcgg tggcgaaggt gtcgcgctga gcgtttaccg gcagaaaagc taacagtcag
    10321 ccgtcgtatc cagaaattaa aaaccgggca atgccctaat gctggtcact taaggtgatc
    10381 agccgttttt ctcgccgaat atttctgtgc atttactctt cgtttccgag ggtaacttcg
    10441 ctctcttctc ccccggtaat acccgccatt ttctttctca tacaacgttg ttagctcgcc
    10501 actcctgccg caccagttct ggcaacttgc tgcgtaacgt ccagcgactg tccaacattc
    10561 cctgtttcac ttcccgataa ccccgttcga ttttccatct gtgtcgatat aagtcactga
    10621 tattattgcc cggatagcga ttctggtctg tacgtacgac ttgatattgt gtgtgtgttt
    10681 tcagcggctg aaggcagcgc tgttttccgg cgtgtctgcc gtgcgccaga caacaccatc
    10741 catggattca agaggaagtt tgtgtttgcg gagcgtg
//
