LOCUS       NZ_CP064062.1          10387 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP064062.1 Brucella anthropi strain PBO chromosome 1, complete
            sequence.
ACCESSION   NZ_CP064062
VERSION     NZ_CP064062.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 14:23:02
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 2044115
            Orig. end    :: 2054502
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[2049115:2049502](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     CDS             394..1254
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091:glutamine-binding lipoprotein glnH (Score: 158.1;
                     E-value: 3.6e-48)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1925"
                     /transl_table=11
                     /translation="MPAPTISKRVTELKSALLAATFAVVALAPHEAAAAPQTVKENTLS
                     VGSDLTYPPFVYMKDGKPAGFDVELMEGVAKQLGLAAEFKDTRFTSLITGARANHFDVI
                     ASALYVTPERQKILNFIPYIKAGSSLLVLKDAKFRPANEKELCGKIIGSMQGASWLPKL
                     KALSEDYCAKNNLGAIETREFDTDAQVTQALRSGSVDVEFMDNVVAAELLKKFPDDYAV
                     TSSALIYPIMVGVATTPQNKELFNAMNDAFAQMRKDGSYDALVKTYGMEPVTDDEIKAV
                     VSTAQ"
     PFAM_domain     523..1200
                     /aSDomain="SBP_bac_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00497.22"
                     /description="Bacterial extracellular solute-binding
                     proteins, family 3"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1925_0035"
                     /evalue="2.90E-52"
                     /label="ctg1_1925"
                     /locus_tag="ctg1_1925"
                     /protein_end="269"
                     /protein_start="43"
                     /score="177.4"
                     /tool="antismash"
                     /translation="LSVGSDLTYPPFVYMKDGKPAGFDVELMEGVAKQLGLAAEFKDTR
                     FTSLITGARANHFDVIASALYVTPERQKILNFIPYIKAGSSLLVLKDAKFRPANEKELC
                     GKIIGSMQGASWLPKLKALSEDYCAKNNLGAIETREFDTDAQVTQALRSGSVDVEFMDN
                     VVAAELLKKFPDDYAVTSSALIYPIMVGVATTPQNKELFNAMNDAFAQMRKDGSYDALV
                     KTYG"
     CDS             1315..2847
                     /gene_functions="transport (smcogs) SMCOG1096:polar amino
                     acid ABC transporter, inner membrane (Score: 177.5;
                     E-value: 5.1e-54)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_1926"
                     /transl_table=11
                     /translation="LNYDWHYALGLLADRDFWEASWVVIKLSLLSWLLAAIAGFGLALA
                     KQSSWSVINGPARAYIWFFRSLPLLVLLIFIYSLPQVFPVLRTLLSNAFAAGLIALVIS
                     ETAYMAEIHRGGLLAIHKGQIEAGRALGLSFLGIQRLIVVPQAIRVSLPAMSNELVTIV
                     KLTSLVSVISLAEILLVGQRLYTQNFLVLETLTVVAFFYVLIVTLFDRGLSMLERRSDV
                     IRRGQKARTLSPQEVAAKLSGTEAPVDRAQGKSQQADTIALEATELRKAYGSHEVLKGV
                     SLTVKSGEVISIIGRSGSGKTTFIRLLNGLERLDAGEVKLHGSPFISAELTQSGKHIFR
                     EDVSLIRDVGMVFQSFNLFNHKTVLENMLLAPLYHKIGARADLERQAFHYLNKVGMAAH
                     AEKYPHQLSGGQQQRVAIARALMMSPSIILFDEPTSALDPETVEEVLGVIRSLASEGVT
                     MVIVTHEMNFAFEVSDRILFMDAGKVVCDDTPDKLKSGIYPQLKPFLKNMTV"
     aSDomain        1360..1659
                     /aSDomain="TIGR01726"
                     /aSTool="tigrfam"
                     /description="HEQRo_perm_3TM: amino ABC transporter,
                     permease protein, 3-TM region, His/Glu/Gln/Arg/opine
                     family"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_1926_0011"
                     /evalue="1.50E-19"
                     /identifier="TIGR01726"
                     /label="ctg1_1926"
                     /locus_tag="ctg1_1926"
                     /protein_end="115"
                     /protein_start="15"
                     /score="68.3"
                     /tool="antismash"
                     /translation="DFWEASWVVIKLSLLSWLLAAIAGFGLALAKQSSWSVINGPARAY
                     IWFFRSLPLLVLLIFIYSLPQVFPVLRTLLSNAFAAGLIALVISETAYMAEIHRG"
     PFAM_domain     1432..1971
                     /aSDomain="BPD_transp_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00528.24"
                     /description="Binding-protein-dependent transport system
                     inner membrane component"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1926_0036"
                     /evalue="5.30E-15"
                     /label="ctg1_1926"
                     /locus_tag="ctg1_1926"
                     /protein_end="219"
                     /protein_start="39"
                     /score="55.7"
                     /tool="antismash"
                     /translation="FGLALAKQSSWSVINGPARAYIWFFRSLPLLVLLIFIYSLPQVFP
                     VLRTLLSNAFAAGLIALVISETAYMAEIHRGGLLAIHKGQIEAGRALGLSFLGIQRLIV
                     VPQAIRVSLPAMSNELVTIVKLTSLVSVISLAEILLVGQRLYTQNFLVLETLTVVAFFY
                     VLIVTLFDRGLSMLERR"
     PFAM_domain     2146..2619
                     /aSDomain="ABC_tran"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00005.29"
                     /description="ABC transporter"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1926_0037"
                     /evalue="4.20E-32"
                     /label="ctg1_1926"
                     /locus_tag="ctg1_1926"
                     /protein_end="435"
                     /protein_start="277"
                     /score="111.6"
                     /tool="antismash"
                     /translation="LKGVSLTVKSGEVISIIGRSGSGKTTFIRLLNGLERLDAGEVKLH
                     GSPFISAELTQSGKHIFREDVSLIRDVGMVFQSFNLFNHKTVLENMLLAPLYHKIGARA
                     DLERQAFHYLNKVGMAAHAEKYPHQLSGGQQQRVAIARALMMSPSIILFDEPTS"
     CDS             2851..3501
                     /gene_functions="regulatory (smcogs) SMCOG1071:GntR family
                     transcriptional regulator (Score: 129.8; E-value: 2.1e-39)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_1927"
                     /transl_table=11
                     /translation="MNQPATLRDPAFIRVANAIRDDILKGRLLSGTSLIEAELINVYDV
                     SRNTLREAMQLLRQQGLVSQEPNKSVRVKRLTETDIRDIFVVRRVVELGALRGRNSVPA
                     DWLERLNEVIRNESEATARADWTAVARQSLLFHQEIVSLHGSPIFDQMFSLLVTQLRLV
                     FVTGNEQAFHEPWLKREAEICRLIAAGDCEAASGQLENYLADSEKMLTALVRN"
     PFAM_domain     2890..3069
                     /aSDomain="GntR"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00392.23"
                     /description="Bacterial regulatory proteins, gntR family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1927_0038"
                     /evalue="2.00E-13"
                     /label="ctg1_1927"
                     /locus_tag="ctg1_1927"
                     /protein_end="73"
                     /protein_start="13"
                     /score="49.9"
                     /tool="antismash"
                     /translation="RVANAIRDDILKGRLLSGTSLIEAELINVYDVSRNTLREAMQLLR
                     QQGLVSQEPNKSVRV"
     PFAM_domain     3097..3459
                     /aSDomain="FCD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07729.14"
                     /description="FCD domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1927_0039"
                     /evalue="4.60E-10"
                     /label="ctg1_1927"
                     /locus_tag="ctg1_1927"
                     /protein_end="203"
                     /protein_start="82"
                     /score="40.1"
                     /tool="antismash"
                     /translation="DIFVVRRVVELGALRGRNSVPADWLERLNEVIRNESEATARADWT
                     AVARQSLLFHQEIVSLHGSPIFDQMFSLLVTQLRLVFVTGNEQAFHEPWLKREAEICRL
                     IAAGDCEAASGQLENYL"
     CDS             3678..4967
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105:amidase (Score: 292.1; E-value: 1.4e-88)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1928"
                     /transl_table=11
                     /translation="MACNRVEKLDLALSDYAARDWNAREVFLNRRFEAARTELGHWQAL
                     SETNRELPPLAGLTMTVKACFDTEGWVTSCGSKALQEAAPASASAALVRRLRMGGATLL
                     AQTNMTEFAYGALGVNTHFGTPRTPLDPAGEAIAGGSSSGAAVAVARGYSDFAICSDTS
                     GSARIPAAFCGVVGFKPSRGRYETDGMHWLSTSFDVPGIISTSVAHCRLIDDVATGIPA
                     RKPRRSKQIRLAVPRLLSETALDNTVEDLFGACRSALEATGIVVQDIELPSLIESARIA
                     VDGGMIGAEAYALHRERLAVDFDSYDPLVGTRIQKGRENPAYLYVNALNALAACREQFD
                     AEIEGFDGFILPTVPMLPPALATLADEETYLALNRRAFSLTEFANRLDLPSISLPIGKH
                     PAGLMLTGLRGEDETLLAIAEVLEPLAAKF"
     PFAM_domain     3765..4925
                     /aSDomain="Amidase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01425.23"
                     /description="Amidase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1928_0040"
                     /evalue="2.00E-70"
                     /label="ctg1_1928"
                     /locus_tag="ctg1_1928"
                     /protein_end="416"
                     /protein_start="29"
                     /score="238.1"
                     /tool="antismash"
                     /translation="RRFEAARTELGHWQALSETNRELPPLAGLTMTVKACFDTEGWVTS
                     CGSKALQEAAPASASAALVRRLRMGGATLLAQTNMTEFAYGALGVNTHFGTPRTPLDPA
                     GEAIAGGSSSGAAVAVARGYSDFAICSDTSGSARIPAAFCGVVGFKPSRGRYETDGMHW
                     LSTSFDVPGIISTSVAHCRLIDDVATGIPARKPRRSKQIRLAVPRLLSETALDNTVEDL
                     FGACRSALEATGIVVQDIELPSLIESARIAVDGGMIGAEAYALHRERLAVDFDSYDPLV
                     GTRIQKGRENPAYLYVNALNALAACREQFDAEIEGFDGFILPTVPMLPPALATLADEET
                     YLALNRRAFSLTEFANRLDLPSISLPIGKHPAGLMLTGLRGEDETLL"
     PFAM_domain     5001..5378
                     /aSDomain="Ectoine_synth"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF06339.14"
                     /description="Ectoine synthase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1929_0041"
                     /evalue="4.10E-54"
                     /label="ctg1_1929"
                     /locus_tag="ctg1_1929"
                     /protein_end="126"
                     /protein_start="0"
                     /score="182.3"
                     /tool="antismash"
                     /translation="MIVVTSDEIAGTARDASGPGWESRRMIVRDDGMGHSVHETRVKEG
                     AELHLHYKNHFETNYCFSGEGEVEHKLSGEVFPIRPGTIYALNENDPHILRATKGDLVL
                     VCVFTPPLAGNEVHREDGSYAP"
     CDS             5001..5387
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_1929"
                     /sec_met_domain="ectoine_synt (E-value: 3.3e-38, bitscore:
                     127.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVVTSDEIAGTARDASGPGWESRRMIVRDDGMGHSVHETRVKEG
                     AELHLHYKNHFETNYCFSGEGEVEHKLSGEVFPIRPGTIYALNENDPHILRATKGDLVL
                     VCVFTPPLAGNEVHREDGSYAPAD"
     CDS             complement(5459..6319)
                     /locus_tag="ctg1_1930"
                     /transl_table=11
                     /translation="MKTNSITPRPADAAARMAVKPVICYPVDTLPRPDLDAYRALRDKL
                     VLVERITVQPRDAECWSVLAGHFCRIVCSEGPQVGDLNLFNADDLNEKFYSGKTRALNG
                     THVGLGDQLFSSFPYLRPMATITHDSLDWYGFDEFGGAVHDVIGTRCDPYTNNLLSHGG
                     QYHHCCHSNLTRAFAKQTGKPLHEAETYIHDVLNVFMCTGFTRDTGQYFMKASPVRPGD
                     YLEFFAEINLLGCLSACPGGDCSAEHSSDTAACHPMVVEIYKPERLPEGWAPPALNGYD
                     RTHGF"
     PFAM_domain     complement(5621..6166)
                     /aSDomain="DUF1989"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF09347.12"
                     /description="Domain of unknown function (DUF1989)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1930_0042"
                     /evalue="9.10E-53"
                     /label="ctg1_1930"
                     /locus_tag="ctg1_1930"
                     /protein_end="233"
                     /protein_start="51"
                     /score="178.4"
                     /tool="antismash"
                     /translation="TVQPRDAECWSVLAGHFCRIVCSEGPQVGDLNLFNADDLNEKFYS
                     GKTRALNGTHVGLGDQLFSSFPYLRPMATITHDSLDWYGFDEFGGAVHDVIGTRCDPYT
                     NNLLSHGGQYHHCCHSNLTRAFAKQTGKPLHEAETYIHDVLNVFMCTGFTRDTGQYFMK
                     ASPVRPGDYLEFFAEINLL"
     CDS             complement(6621..8045)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017:aldehyde dehydrogenase (Score: 482.5; E-value:
                     1.7e-146)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_1931"
                     /transl_table=11
                     /translation="MNTEMLIGSRFEAGTETEETVLNPKTGETVLKLPEASLGQIDAAV
                     AAADKAFKTWSRTTPAERSNYLLKIADAIERDAEGFATLEALNCGKPINAVINDEIPAI
                     VDCYRFFAGAVRNLHGPLAAEYIPNHTSMIRRDPVGIVGSIAPWNYPLMMMAWKLAPAI
                     AGGNTVVFKPSEQTPLTALKMARLLADILPEGVVNVVLGRGETVGNALINHPRIDMVSI
                     TGDVATGKKVLQAAAKTVKRTHLELGGKAPVIVFDDADIEAVVNGIRTFGYYNAGQDCT
                     AACRIYADARIYDRFVADLSSAVSSIRYNLDDDSQNEIGPLISRRQRDRVGSFVARARE
                     HKHIEVTAGGNTSGANGFFFEPTVIAGALQEDEIVRREVFGPVVSVTRFSEVDEAVRWA
                     NDSDYGLASSVWTKDVSKGMQAAARLQYGCTWINTHFMLTNEMPHGGLKQSGYGKDMSI
                     YALEDYTAVRHVMIAH"
     PFAM_domain     complement(6636..7997)
                     /aSDomain="Aldedh"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00171.24"
                     /description="Aldehyde dehydrogenase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1931_0043"
                     /evalue="1.90E-160"
                     /label="ctg1_1931"
                     /locus_tag="ctg1_1931"
                     /protein_end="470"
                     /protein_start="16"
                     /score="534.5"
                     /tool="antismash"
                     /translation="TEETVLNPKTGETVLKLPEASLGQIDAAVAAADKAFKTWSRTTPA
                     ERSNYLLKIADAIERDAEGFATLEALNCGKPINAVINDEIPAIVDCYRFFAGAVRNLHG
                     PLAAEYIPNHTSMIRRDPVGIVGSIAPWNYPLMMMAWKLAPAIAGGNTVVFKPSEQTPL
                     TALKMARLLADILPEGVVNVVLGRGETVGNALINHPRIDMVSITGDVATGKKVLQAAAK
                     TVKRTHLELGGKAPVIVFDDADIEAVVNGIRTFGYYNAGQDCTAACRIYADARIYDRFV
                     ADLSSAVSSIRYNLDDDSQNEIGPLISRRQRDRVGSFVARAREHKHIEVTAGGNTSGAN
                     GFFFEPTVIAGALQEDEIVRREVFGPVVSVTRFSEVDEAVRWANDSDYGLASSVWTKDV
                     SKGMQAAARLQYGCTWINTHFMLTNEMPHGGLKQSGYGKDMSIYALEDYTAVRHV"
     CDS             complement(8062..8877)
                     /gene_functions="transport (smcogs) SMCOG1069:putative ABC
                     transporter permease protein (Score: 151.3; E-value:
                     5.7e-46)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_1932"
                     /transl_table=11
                     /translation="MRSDRNNRASFGLKLAAGLGLAFLHLPILLIFVYAFTTEEKSYQW
                     PPPGFTLQWFEVTWNRADVWSSLALSVQVATIATLIALVLGTLCAAAVSRTHFFGRESI
                     SLLVILPIALPGVVTGIALRSAFSLFDIPFSMWTIVLGHATFCVVVVYNNAVARFRRTS
                     GSLIEASMDLGANGFQTFRYVILPNIGTALLAGGMLAFALSFDEVIVTTFTGGQQMTLP
                     IWMLQELIRPRQRPVTNVVAMVVVLVTFLPILAAYYLTRDGDQVAGHGK"
     PFAM_domain     complement(8107..8631)
                     /aSDomain="BPD_transp_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00528.24"
                     /description="Binding-protein-dependent transport system
                     inner membrane component"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1932_0044"
                     /evalue="1.30E-12"
                     /label="ctg1_1932"
                     /locus_tag="ctg1_1932"
                     /protein_end="257"
                     /protein_start="82"
                     /score="48.0"
                     /tool="antismash"
                     /translation="LVLGTLCAAAVSRTHFFGRESISLLVILPIALPGVVTGIALRSAF
                     SLFDIPFSMWTIVLGHATFCVVVVYNNAVARFRRTSGSLIEASMDLGANGFQTFRYVIL
                     PNIGTALLAGGMLAFALSFDEVIVTTFTGGQQMTLPIWMLQELIRPRQRPVTNVVAMVV
                     VLVTFLPILAAY"
     CDS             complement(8867..9850)
                     /gene_functions="transport (smcogs) SMCOG1067:putative ABC
                     transporter permease protein (Score: 180.4; E-value:
                     7.9e-55)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_1933"
                     /transl_table=11
                     /translation="MTGVQASTLANSGTNPAASILPQRGGMLGRLSDLFWRRPGLLLFL
                     MITPPLLWLGIIYIGSLIALLLQSFFSVDDFSGLIDYNFTLATYAQLFNSANLDIIIRT
                     VSMAALVTVASAFIAFPIAYYAARYARGRWKALFYLGIMLPLWSSYLVKVYAWKLILAK
                     EGILTWIFAKLHLSWLLDGILSLPVIGGNSLSVSYLGTFIVFVYIWLPYMILPTQAALE
                     RVPGNLVEASSDLGATPAQTFRTVLMPLAMPGIIAGSIFTFSLTLGDYIIPQIIGSSRL
                     FIGQAVYTQQGTAGNVPLAAAFSVVPIVIMAIYLWIAKKQGAFDAL"
     PFAM_domain     complement(8897..9493)
                     /aSDomain="BPD_transp_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00528.24"
                     /description="Binding-protein-dependent transport system
                     inner membrane component"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_1933_0045"
                     /evalue="1.20E-16"
                     /label="ctg1_1933"
                     /locus_tag="ctg1_1933"
                     /protein_end="318"
                     /protein_start="119"
                     /score="61.1"
                     /tool="antismash"
                     /translation="FPIAYYAARYARGRWKALFYLGIMLPLWSSYLVKVYAWKLILAKE
                     GILTWIFAKLHLSWLLDGILSLPVIGGNSLSVSYLGTFIVFVYIWLPYMILPTQAALER
                     VPGNLVEASSDLGATPAQTFRTVLMPLAMPGIIAGSIFTFSLTLGDYIIPQIIGSSRLF
                     IGQAVYTQQGTAGNVPLAAAFSVVPIVIMAIYLWIA"
ORIGIN
        1 atcatacagc tcggtagtca ggtggatgaa agagtgcaga gtttgacccg tctccaggat
       61 atttttggcg atttcgagag ttaaagcatt tccagcaaac gtgcggagcg gttttgcatg
      121 aggcaaatgc aatgaaccaa atacttagag cggtttcagc gactccgttt taactgggag
      181 cgctctggcg aaaagctacc cttcaaaagt ctgcaaaact tgcaaagacc agttttggaa
      241 accagttccg agaaatgaaa ctccgatacc gcctaatcgc atcggaagag atgagcatac
      301 gtgtcttcat cgctcccacg tgaagacata ttgacatcgc acggcccatc tcttaattgt
      361 tcaacaatat tattgaacaa aaaagggaac gatatgcctg ctccaacaat ctcaaaacgc
      421 gtgactgaac tgaaatcagc cttgctcgct gccacatttg ccgtcgttgc gttggccccg
      481 catgaggccg cagccgcgcc tcagaccgtc aaggaaaaca cactgtcggt cggatctgac
      541 ctgacctatc ctcccttcgt ctacatgaag gacggcaagc cggcaggttt cgatgtcgag
      601 ctgatggaag gggtggcaaa gcagcttggg cttgctgcag agttcaagga cacgcgcttc
      661 accagcctca tcactggtgc acgcgccaac catttcgatg tcattgcatc ggcgctttac
      721 gtcacgcctg agcggcagaa gattctcaat ttcatcccgt acatcaaggc tggctcgtcc
      781 ctgctcgtgc tgaaggacgc caagttccgc ccggcgaacg aaaaggagct gtgcggcaag
      841 atcatcgggt cgatgcaagg agcgtcctgg ctcccgaagc tgaaggcact gtcggaagat
      901 tattgcgcca agaacaatct cggcgccatc gagacacgcg aattcgatac ggatgcgcag
      961 gtcacgcagg cattgcgcag cggctcggtc gacgtggaat tcatggataa tgtcgttgct
     1021 gccgaactgc tgaagaagtt ccccgacgat tatgccgtta catccagtgc gctcatctat
     1081 ccgatcatgg tgggtgtagc caccaccccg cagaacaaag agcttttcaa tgccatgaac
     1141 gatgctttcg cccagatgcg aaaggatggt tcctatgacg cgctcgtcaa aacctatggc
     1201 atggagcctg tcaccgacga tgaaatcaaa gcggtcgttt ccacggctca ataaaccgga
     1261 agcggggcgg actggcccca tttccctctg catggccgaa acgggaggct tcgcttgaac
     1321 tacgattggc attacgcgct cggtctgctt gcagaccggg atttctggga ggcgagctgg
     1381 gttgtgatca agcttagcct gctgagctgg cttctggcgg cgattgcggg cttcggtctg
     1441 gcactggcaa agcaatcatc atggtcggtg atcaacggtc ccgcacgggc ctatatctgg
     1501 ttcttccgaa gccttccgct tctggtactg ctgattttca tctacagcct gccgcaggtc
     1561 tttccggtcc ttcgcaccct cctgtcgaat gctttcgcgg ctggcctgat cgcgcttgtc
     1621 atcagtgaaa ccgcttatat ggcagaaatc catcggggtg gtttgctcgc cattcataaa
     1681 ggccagatcg aggcggggcg tgcgctgggt ctgagcttcc tcggtatcca gcgcctgatc
     1741 gtcgttccac aggcaatccg ggtttcatta cccgcaatgt cgaatgaact cgtcaccatc
     1801 gtcaagctca cttctctggt ttcggtcatt tcgcttgccg aaatcctgct tgtcggccag
     1861 cggctctata cgcagaattt tctggtgctc gagacgctca ccgtcgtcgc cttcttctac
     1921 gtcctcatcg tcacgctgtt tgatcgcggt ctttcaatgc tggaacgccg ctcggatgtg
     1981 atacggcgcg gtcagaaggc acgcaccttg tcgccacagg aggttgcggc aaaactgtcc
     2041 ggcactgaag ctcccgtcga tcgcgcgcaa ggcaaatccc agcaagccga caccattgcc
     2101 ctggaagcaa ctgaacttcg caaggcctat ggttcgcatg aagtgttgaa gggcgtcagc
     2161 ctgacggtga aatccggtga ggtcatctcc atcatcggtc gttcgggctc gggcaagacg
     2221 accttcattc gcctgctcaa cggactggaa cgtctcgatg ccggtgaagt aaaactgcac
     2281 ggctcgccgt tcatctcggc cgaattgacg caatcgggca agcatatttt ccgggaagac
     2341 gtttccctga tccgcgatgt cggcatggtg ttccagagct tcaatctttt caatcacaag
     2401 actgtgcttg aaaacatgct cctcgcgcct ctgtaccaca agatcggtgc gcgagccgac
     2461 cttgagcggc aagcctttca ttatctgaac aaggtgggca tggccgccca tgcggaaaaa
     2521 tatccgcacc agctttcggg cggtcagcag cagcgcgttg caatcgctcg cgccctgatg
     2581 atgtcgccaa gcatcattct gttcgatgaa cccacttcgg cactcgaccc ggaaaccgtc
     2641 gaggaagtgc tgggcgttat ccgctcgctc gcaagcgaag gcgtgacgat ggtcatcgta
     2701 acccatgaaa tgaacttcgc tttcgaagtc tccgacagaa ttctctttat ggacgcgggc
     2761 aaggtcgtct gcgacgatac gccggataaa ctcaaatccg gtatctatcc gcagctgaaa
     2821 cctttcctga agaatatgac ggtctgaaac atgaaccagc ctgccaccct tcgtgatccc
     2881 gcattcattc gcgtcgccaa tgccatacgc gacgacattc tcaaaggccg cctgttgagc
     2941 ggcacgagcc tgatcgaggc cgaactgatc aatgtctacg atgtatcgcg caacaccctg
     3001 cgcgaagcca tgcagcttct gcgtcagcag gggctggtct cgcaggagcc gaacaagagc
     3061 gtccgcgtga aacggctaac ggaaacggac attcgcgaca tcttcgtggt gcgccgggtc
     3121 gtcgagctcg gcgcactccg tgggcgaaac tcggttcccg ccgactggct ggaaaggctc
     3181 aacgaggtga tccgcaatga aagtgaagcg accgcccgcg cggactggac ggcggtggcc
     3241 cgccagagcc ttctctttca ccaggaaatc gtatcgctgc atggtagtcc tatcttcgat
     3301 cagatgttct ccctgctcgt gacccagctg cgtctggttt tcgtcaccgg caacgaacag
     3361 gctttccatg aaccttggtt gaaaagagaa gccgaaatct gccgcctcat cgcagcgggt
     3421 gactgcgaag ccgcctccgg tcagcttgag aactatctcg cggattcaga gaaaatgctg
     3481 acggcattgg tccggaactg atcgcagccg attgcgatag agcgcgtttc gcttcgattg
     3541 aagcagatcg gcgctctaac ctcttatatt tcaagcataa tcttatcgat cctcgtttca
     3601 cttcggtcgg attatgctct aacgggacat gctttatgaa acacgatccg caagacaaat
     3661 ccggattggg atctgccatg gcttgcaacc gggttgaaaa gctcgacctg gcgctgtccg
     3721 actatgcggc gcgtgactgg aatgcgcgag aggtttttct caaccggcgg ttcgaggcgg
     3781 cccgcaccga gctcggccat tggcaagcgc tcagtgaaac caatcgtgaa ctcccgccgc
     3841 ttgcaggact gacgatgacg gtcaaggcct gcttcgacac tgaaggctgg gtgaccagct
     3901 gtggttcgaa agcattacaa gaggcagctc ctgcaagtgc aagcgccgct ctggtgcgca
     3961 ggcttcgcat gggtggcgca acactgctcg cgcagacaaa catgaccgaa tttgcttatg
     4021 gcgcgctcgg ggtcaacaca catttcggca cgccacgcac gccgctcgac cctgccggag
     4081 aggcgatcgc tggcggctct agttctggtg cagcggtggc cgtcgcgcgc ggatatagcg
     4141 atttcgccat ctgctccgat accagcggtt cagcgcgcat tccagcggct ttctgtggcg
     4201 tcgtgggctt caaaccaagc cgcggacgct acgaaaccga tggaatgcac tggttgtcga
     4261 ccagctttga cgtgccgggg atcatcagca cttcggtcgc ccattgtcgc ctgatcgatg
     4321 atgttgccac cggcatcccg gcaaggaaac cgcggcgctc aaagcagata agactggcag
     4381 ttccccgcct gctttcggaa accgctctcg acaataccgt tgaagatctt ttcggggctt
     4441 gccggtccgc tctggaagca acaggaattg tcgtccagga tatcgaactt ccaagcttga
     4501 tcgagagtgc caggattgcc gtcgatggcg ggatgatcgg tgccgaggct tacgcgcttc
     4561 atcgcgagcg gctcgccgtc gatttcgata gttatgatcc cttggtcgga acgcgcattc
     4621 aaaaaggtcg ggaaaatccc gcctatctct atgtgaatgc attgaacgcc cttgctgcct
     4681 gccgtgagca gttcgacgcg gaaatcgagg gttttgacgg tttcattctg ccgaccgttc
     4741 ccatgctgcc gcccgccttg gccacgcttg cagacgagga aacctatctg gcgctcaacc
     4801 ggcgtgcatt ctcgctgaca gaattcgcca atcgtcttga tctgcccagc atctcgctac
     4861 caatcgggaa gcaccctgcc ggtctcatgc tgaccggact gcgcggcgag gacgagacgc
     4921 tactggccat cgcagaagta ctcgagccgc tggccgccaa gttttaacag cctttttgac
     4981 actgaaatga ggagaatact atgattgtcg tgacatccga tgaaatcgcc ggcaccgcac
     5041 gcgatgcttc cggacccggt tgggaaagcc gccgcatgat cgtgcgtgac gacggcatgg
     5101 gccattcggt ccacgaaacc cgcgtcaagg aaggcgctga actgcacctg cactacaaga
     5161 accacttcga aaccaattat tgcttttccg gtgaaggcga agtcgaacac aagttgagcg
     5221 gcgaagtgtt tccgatccgc cccggcacca tctatgcgct gaacgaaaac gacccgcata
     5281 tcctgcgcgc gaccaagggc gaccttgtgc tggtctgtgt tttcacgcca ccgctggctg
     5341 gcaatgaagt gcaccgcgaa gatggcagct acgctcccgc cgactgaagt atagagtttc
     5401 ggcaattgaa agaaaacgcc gcgattgatc gcggcgtttt tgttgatctg ctgttggttc
     5461 agaaaccgtg tgtgcgatca tatccgttga gagcaggcgg cgcccagcct tccggcagac
     5521 gttctggctt gtaaatctct accaccatcg gatggcaggc ggcagtatcg gaagaatgtt
     5581 ctgccgaaca gtcacccccg ggacaggccg acagacagcc aagaagattg atctcggcaa
     5641 agaattccag atagtcgccc ggtcgaacag ggcttgcctt catgaaatat tgcccagtat
     5701 cccgggtgaa gcccgtgcac atgaacacgt tgagcacgtc gtgaatatag gtttcggcct
     5761 cgtgcagcgg cttgcccgtc tgcttcgcaa aagcgcgcgt gaggttggaa tggcagcagt
     5821 gatggtactg cccgccatga ctcagaagat tattggtata aggatcgcag cgcgtgccga
     5881 tcacgtcatg caccgcgccg ccaaattcgt cgaaaccata ccagtccaga ctgtcgtgag
     5941 tgatggtggc catcggacgc aagtaaggaa aactggagaa cagctggtct cccagcccca
     6001 catgggttcc gttgagcgcg cgcgtcttgc cgctatagaa tttctcgttg aggtcgtccg
     6061 cattgaacag gttgagatca ccgacctgcg gtccctcgct gcatacgata cggcagaaat
     6121 gcccggccag aacggaccag cactctgcat ccctcggctg cacggtgatg cgctcgacaa
     6181 gaacaagttt gtcgcgaagg gcgcgatagg catcgagatc aggtcgtggc agcgtatcga
     6241 ccggataaca gatcactggt ttgacggcca tgcgagcagc ggcatcggca ggccgcggcg
     6301 tgatcgagtt tgtcttcatg aagcgctctg tcgttctctt gcgtttggat cgccacgata
     6361 cgccaccgcg cattccatga cggacaagtt ctaacatgga agatatcgtt caacaatatt
     6421 gatgaataat cataggcact gccatacttt atgtcagcct tgtttgaaga gcttcaaagc
     6481 gacatgcgaa cagcgacgga acaatacatc tgtggtgcgt aatagaacgc cgatctgatt
     6541 gaatcagatc ggcgttctaa ctgtttgttt tggcgcagat cttgttcaca agaaatgcct
     6601 ttcacttctc ggcatttccc ctagtgagct atcatgacat gacgcacggc ggtgtaatct
     6661 tccagcgcgt aaatcgacat gtccttgccg tagcccgact gtttcaggcc gccatgcggc
     6721 atctcgtttg tcagcatgaa atgggtgttg atccatgtgc agccatattg caggcgggct
     6781 gcggcctgca tgcccttgct gacatccttg gtccagaccg acgaggcaag gccgtaatca
     6841 ctgtcgttgg cccatcgcac ggcttcatcg acttcgctga agcgtgtgac cgatacaacc
     6901 ggaccgaaga cttcccggcg cacgatttca tcttcctgca atgctccggc gatgacggtc
     6961 ggttcgaaga aaaagccatt ggcaccggac gtattaccac ctgcggtcac ctcgatatgc
     7021 ttgtgctcgc gagcgcgggc gacgaaactt ccgacacggt cgcgctgtcg gcgggaaatg
     7081 agcggtccga tctcgttctg gctgtcgtcg tcgagattat agcgaatact ggaaacggcc
     7141 gacgacaggt cagcgacgaa acggtcgtag atgcgtgcat cggcatagat gcggcaggca
     7201 gcggtgcagt cctgtccggc attgtaatag ccgaaggtgc ggataccgtt gacgaccgct
     7261 tctatgtccg catcgtcgaa gacgatgacc ggcgctttgc cgccaagctc cagatgagtg
     7321 cgcttcacgg ttttggccgc ggcctgcaag accttcttgc cggtggcgac atcaccggtg
     7381 atcgacacca tgtcgatccg cggatggttg atcagcgcgt tgccgacggt ttcacctcgc
     7441 cccagaacga cattgacaac gccctcgggc agaatatccg ccagcagcct ggccattttc
     7501 agcgcggtca gaggcgtctg ttcggacggt ttgaaaacca ccgtattacc gcctgcaatg
     7561 gctggcgcca gtttccatgc catcatcatc aaaggataat tccaaggagc gatggagccg
     7621 acaatgccaa ccggatcacg acggatcatc gatgtgtggt tcgggatata ttcggcggca
     7681 agcggtccat gaagattacg cactgcaccc gcaaagaagc gataacaatc cacgatggcc
     7741 ggaatctcgt cattgatgac agcattgatc ggcttgccgc aattgagcgc ttccagcgtg
     7801 gcgaaccctt cagcgtcccg ctcaatcgca tcggcgattt tgaggagata attggaacgt
     7861 tcggcgggtg tggtgcgcga ccaggtcttg aatgccttgt cggcggcggc gactgcagcg
     7921 tcgatctgtc ctagcgatgc ctcgggcagc ttgagaacgg tctctccggt cttgggattg
     7981 aggacggttt cctcggtctc cgtacccgct tcaaagcgcg agccaatcag catttcggtg
     8041 ttcatgtcgt tctcccttgt gtcatttccc gtgaccggcg acctgatcgc catcgcgggt
     8101 gagatagtag gctgcgagga tgggcaggaa ggtcaccaga accaccacca tggccaccac
     8161 attggtgacg gggcgctggc gcggacggat cagttcctga agcatccaga tcggcagggt
     8221 catctgctgg ccgccagtaa atgtggtgac gatcacctca tcgaaggaca gtgcgaaggc
     8281 gagcatgccg cctgcgagca gggccgttcc gatattcggc aggatgacat agcggaacgt
     8341 ctggaacccg ttggcgccaa gatccatcga ggcctcgatc agcgaaccgg aggtgcgccg
     8401 gaaacgtgca accgcgttgt tatagacgac caccacgcag aaggtcgcgt gaccgagcac
     8461 gatggtccac atcgaaaacg gaatgtcgaa cagcgagaag gccgagcgca aggcgatgcc
     8521 ggttacaacg ccgggcaaag ctatgggcag gatgaccagc agcgaaatgc tttcgcgtcc
     8581 gaagaaatgc gttcggctga cagcggcggc gcacaacgtt ccaagcacga gcgcaatcag
     8641 ggtcgcaatc gtcgccacct gcacggagag cgccagcgac gaccaaacat cggcgcgatt
     8701 ccaggtgact tcgaaccatt gcagcgtgaa acccggcggc ggccattgat agctcttctc
     8761 ttccgtcgtg aaggcataga cgaagatgag caggatcggc agatgcagga aggcgaggcc
     8821 cagtccggca gcaagcttca atccgaaaga agcgcggttg ttacgatcag agcgcatcga
     8881 aagccccctg tttcttggcg atccacaaat aaatggccat gatgacgatc ggcacgacgg
     8941 agaaggcagc cgccagcggc acattgcctg ccgttccctg ctgggtatag acagcctgtc
     9001 cgatgaacag gcgcgacgag ccgatgatct gcggaatgat gtaatcgcca agtgtcagcg
     9061 aaaaggtgaa gatcgagccg gcgatgatgc ccggcatggc aagcggcatg agcacggttc
     9121 ggaaggtctg cgccggcgtt gccccgagat cggacgacgc ctcgaccaga ttgccgggca
     9181 cacgctcaag tgctgcctgt gtcggcagga tcatataggg cagccagata tagacgaaga
     9241 cgatgaaggt gccgagatag ctgaccgaca gcgaattgcc gccgatgacg ggtaaggaca
     9301 ggatgccgtc aagcagccag gacaaatgca gcttcgcaaa tatccaggtc aggatgcctt
     9361 ccttggcaag gatcagtttc cacgcataga ccttgaccag atagctcgac cagagcggca
     9421 gcatgatgcc gagataaaac agcgccttcc agcgtccgcg tgcataacgg gcggcataat
     9481 aggcgatggg aaaggcgatg aaggccgatg cgaccgtgac cagcgcagcc atcgagacgg
     9541 tgcgtatgat gatgtcgaga ttggccgagt tgaagagctg cgcataggtg gcaagcgtga
     9601 aattatagtc gatcagaccg gagaaatcat cgaccgagaa gaagctctgc aacagaagtg
     9661 cgatcagcga gccgatatag atgatgccca gccacaaaag cggcggcgta atcatgagaa
     9721 acagcagcaa tcccggacga cgccagaaca ggtcggagag gcgaccgagc attccgccgc
     9781 gctgcggcaa gatggaagcc gccggattgg tcccggagtt cgcgagtgtg gaagcctgca
     9841 caccggtcat gctgcttgat ccatgtaatg aacgtcaccc ggattccaga caatacggat
     9901 atccgatccg gtctcgggaa cggttgcacc agcaggcagc atgacatgca gtctggcgcc
     9961 ttcagcttca acaccgagac gtgtagcgga cccaaggaag ctggcggttt cgatccgcgc
    10021 cgcgaccccg accttatcac tcttgtctgc aaggcgaatg gattccgggc ggaggcttgc
    10081 ccaacgtcgt tcgccgccga atgcagccat aacctccggc gcgaccacat tggaggagcc
    10141 gacaaagtcg gcaacaaacc gggtttgcgg gcggttatat atttcctgtg gcgttccggc
    10201 ctgaacgata cgaccattgt tgaagacagc gatacggtcc gccatggaca gcgcttcgcc
    10261 ctgatcatgg gtcacgaaga caaaggtgat accgagcgcc ttttgcaggc tcttcagctc
    10321 ttcctgcatc tgctcgcgca gtttgaggtc gagagcaccg agcggctcat cgagaagcag
    10381 cactttg
//
