LOCUS       c00005_NZ_APBN..       10849 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_APBN01000005.1 Brevibacillus borstelensis AK1 seq_num_005, whole
            genome shotgun sequence.
ACCESSION   NZ_APBN01000005
VERSION     NZ_APBN01000005
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:26:13
            Original ID  :: NZ_APBN01000005.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 230834
            Orig. end    :: 241683
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10849
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[235834:236683](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5849)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..10849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193)"
                     /tool="antismash"
     CDS             1660..2835
                     /NRPS_PKS="Domain: Aminotran_1_2 (32-383). E-value: 4e-61.
                     Score: 198.9. Matches aSDomain:
                     nrpspksdomains_ctg5_203_Aminotran_1_2.1"
                     /NRPS_PKS="type: other"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 252.5; E-value:
                     1.2e-76)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg5_203"
                     /transl_table=11
                     /translation="MRIQPSAMVDRLPTQFFATLVAKTNKAIAAGHDIINLGQGNPDLP
                     TPQHIVEELSEQAKRPVHHKYPPFSGRLELKQAVAHWYKEEFGVELDAEEEVAILFGSK
                     TGLVEICQVLLNPGDVALVPDPGYPDYWSGVAVADGRMVMMPLKAENAFLPDYGQLKPE
                     DLEKAKLMFLNYPNNPTAVNAPVEFFEETIRFARKHEIVVCHDFAYGAISFDGKKPVSF
                     MQVPGAKEVGVEFYTLSKTYNMAGWRVGAMVGNKELVRLVNLIQDHYFVSLFGAVQMAA
                     AKAMTGSQQCVHDLVSVYQSRRDVLFAELHRIGWQAKPSEGSFFSWLPVPEGFTSVEFS
                     DLLLEKAHVVVAPGVGFGPTGEGYVRAALLSSEERLAEAVRRIEKLGLFAR"
     aSDomain        1756..2808
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg5_203_Aminotran_1_2.1"
                     /evalue="4.00E-61"
                     /label="ctg5_203_Aminotran_1_2.1"
                     /locus_tag="ctg5_203"
                     /protein_end="383"
                     /protein_start="32"
                     /score="198.9"
                     /tool="antismash"
                     /translation="DIINLGQGNPDLPTPQHIVEELSEQAKRPVHHKYPPFSGRLELKQ
                     AVAHWYKEEFGVELDAEEEVAILFGSKTGLVEICQVLLNPGDVALVPDPGYPDYWSGVA
                     VADGRMVMMPLKAENAFLPDYGQLKPEDLEKAKLMFLNYPNNPTAVNAPVEFFEETIRF
                     ARKHEIVVCHDFAYGAISFDGKKPVSFMQVPGAKEVGVEFYTLSKTYNMAGWRVGAMVG
                     NKELVRLVNLIQDHYFVSLFGAVQMAAAKAMTGSQQCVHDLVSVYQSRRDVLFAELHRI
                     GWQAKPSEGSFFSWLPVPEGFTSVEFSDLLLEKAHVVVAPGVGFGPTGEGYVRAALLSS
                     EERLAEAVRRI"
     PFAM_domain     1756..2808
                     /aSDomain="Aminotran_1_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00155.23"
                     /description="Aminotransferase class I and II"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_203_0001"
                     /evalue="1.30E-58"
                     /label="ctg5_203"
                     /locus_tag="ctg5_203"
                     /protein_end="383"
                     /protein_start="32"
                     /score="199.0"
                     /tool="antismash"
                     /translation="DIINLGQGNPDLPTPQHIVEELSEQAKRPVHHKYPPFSGRLELKQ
                     AVAHWYKEEFGVELDAEEEVAILFGSKTGLVEICQVLLNPGDVALVPDPGYPDYWSGVA
                     VADGRMVMMPLKAENAFLPDYGQLKPEDLEKAKLMFLNYPNNPTAVNAPVEFFEETIRF
                     ARKHEIVVCHDFAYGAISFDGKKPVSFMQVPGAKEVGVEFYTLSKTYNMAGWRVGAMVG
                     NKELVRLVNLIQDHYFVSLFGAVQMAAAKAMTGSQQCVHDLVSVYQSRRDVLFAELHRI
                     GWQAKPSEGSFFSWLPVPEGFTSVEFSDLLLEKAHVVVAPGVGFGPTGEGYVRAALLSS
                     EERLAEAVRRI"
     CDS             complement(3170..3553)
                     /locus_tag="ctg5_204"
                     /transl_table=11
                     /translation="MLVQMFPAFLLVVNRAVEVRNRRKSKSTVGRVFVGKFEPVWIIEL
                     KGLGKLESFPYWNLYDENGKRFDVRTDFAKTYMENSDKGVVLVQTLIVKGMEQPPQKLK
                     LALQTIETRYIDLNWQPPIPPSP"
     CDS             3715..4914
                     /NRPS_PKS="Domain: Aminotran_1_2 (34-386). E-value:
                     1.8e-40. Score: 131.0. Matches aSDomain:
                     nrpspksdomains_ctg5_205_Aminotran_1_2.1"
                     /NRPS_PKS="type: other"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 310.2; E-value:
                     3.4e-94)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg5_205"
                     /transl_table=11
                     /translation="MEYSFSKSTSALSSSAVREILKLTQGNQVLSLAGGLPAEDSFPIE
                     EMREAFNRALDHGAKSLQYGLTDGYIPLREWVSSRMKQKHMNVGVENMLITTGSQQAVD
                     LLCRVYLDEGDVVLVENPTYLAAVQLFQFRGIRAIPVDGDAEGMDMEDLARKIAQYRPK
                     MAYVIPTFANPTGKVWSLEKRLGLLKQCRENNILILEDDPYGEIQFDGEAPYRSIFSLD
                     EHPDGSAVVYTSTFSKIVAPGLRTGWAIGDSQVIQMMVKAKQAIDLQSSTIDQFALYEL
                     LSRFDLESHIQKIRDSYKERMEWMHELLCRQTSGMKWNKPRGGMFLWVELPEHVHSEKL
                     LAQAVKEGVAFVPGIPFYADQPQTNTMRLNYTLLDREHTELAINRLGKAFAEYSQLVAH
                     "
     aSDomain        3817..4872
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg5_205_Aminotran_1_2.1"
                     /evalue="1.80E-40"
                     /label="ctg5_205_Aminotran_1_2.1"
                     /locus_tag="ctg5_205"
                     /protein_end="386"
                     /protein_start="34"
                     /score="131.0"
                     /tool="antismash"
                     /translation="GLPAEDSFPIEEMREAFNRALDHGAKSLQYGLTDGYIPLREWVSS
                     RMKQKHMNVGVENMLITTGSQQAVDLLCRVYLDEGDVVLVENPTYLAAVQLFQFRGIRA
                     IPVDGDAEGMDMEDLARKIAQYRPKMAYVIPTFANPTGKVWSLEKRLGLLKQCRENNIL
                     ILEDDPYGEIQFDGEAPYRSIFSLDEHPDGSAVVYTSTFSKIVAPGLRTGWAIGDSQVI
                     QMMVKAKQAIDLQSSTIDQFALYELLSRFDLESHIQKIRDSYKERMEWMHELLCRQTSG
                     MKWNKPRGGMFLWVELPEHVHSEKLLAQAVKEGVAFVPGIPFYADQPQTNTMRLNYTLL
                     DREHTELAINRL"
     PFAM_domain     3817..4872
                     /aSDomain="Aminotran_1_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00155.23"
                     /description="Aminotransferase class I and II"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_205_0002"
                     /evalue="5.10E-38"
                     /label="ctg5_205"
                     /locus_tag="ctg5_205"
                     /protein_end="386"
                     /protein_start="34"
                     /score="131.3"
                     /tool="antismash"
                     /translation="GLPAEDSFPIEEMREAFNRALDHGAKSLQYGLTDGYIPLREWVSS
                     RMKQKHMNVGVENMLITTGSQQAVDLLCRVYLDEGDVVLVENPTYLAAVQLFQFRGIRA
                     IPVDGDAEGMDMEDLARKIAQYRPKMAYVIPTFANPTGKVWSLEKRLGLLKQCRENNIL
                     ILEDDPYGEIQFDGEAPYRSIFSLDEHPDGSAVVYTSTFSKIVAPGLRTGWAIGDSQVI
                     QMMVKAKQAIDLQSSTIDQFALYELLSRFDLESHIQKIRDSYKERMEWMHELLCRQTSG
                     MKWNKPRGGMFLWVELPEHVHSEKLLAQAVKEGVAFVPGIPFYADQPQTNTMRLNYTLL
                     DREHTELAINRL"
     CDS             complement(5001..5849)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg5_206"
                     /sec_met_domain="Linocin_M18 (E-value: 1.3e-84, bitscore:
                     277.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKLRKYPDSPLTHDEWKQLDETVVDMARRQLVGRRFLDIYGPLG
                     EGIQTITNDIYDESRFGGLNLRGESLEMTQPSKRVSLTIPILYKDFMLYWRDVAQARTL
                     GMPLDMSAAANAAAGCALMEDDLIFNGSPGLDLPGLMNVKGRLTHLKSDWMESGNAFAD
                     IVEARNKLLKMGHSGPYALVVSPELYSLLHRVHKGTNVLEIEHVRNLVTDGVFQSPTIK
                     GRSGVLVATGRHNLDLAIAEDFDTSFLGDEQMNSLFRVYECVVLRIKRPSAICTLEETE
                     E"
     PFAM_domain     complement(5058..5840)
                     /aSDomain="Linocin_M18"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04454.14"
                     /description="Encapsulating protein for peroxidase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_206_0003"
                     /evalue="7.70E-85"
                     /label="ctg5_206"
                     /locus_tag="ctg5_206"
                     /protein_end="264"
                     /protein_start="3"
                     /score="284.3"
                     /tool="antismash"
                     /translation="LRKYPDSPLTHDEWKQLDETVVDMARRQLVGRRFLDIYGPLGEGI
                     QTITNDIYDESRFGGLNLRGESLEMTQPSKRVSLTIPILYKDFMLYWRDVAQARTLGMP
                     LDMSAAANAAAGCALMEDDLIFNGSPGLDLPGLMNVKGRLTHLKSDWMESGNAFADIVE
                     ARNKLLKMGHSGPYALVVSPELYSLLHRVHKGTNVLEIEHVRNLVTDGVFQSPTIKGRS
                     GVLVATGRHNLDLAIAEDFDTSFLGDEQMNSLFRVYECV"
     CDS             complement(5903..6505)
                     /locus_tag="ctg5_207"
                     /transl_table=11
                     /translation="VQEMSQLHAIFSRTRGYIDRFAEIIQPIIDAAQDEHTRLYYHHIL
                     EEEEQRLGRLQELIPYIGSLDEAKKAEQLSDRELSRLLSDINLERFGLHNFREHLELSL
                     YEFKDEETRQLLNSMRESTQEDYLRVKEIMAALSERFSDVHRPDLTDHDEGYDIHQVDH
                     LKASSSPAVANTGKTAAAPAAKTGRKGLTVGSLKGMF"
     aSDomain        complement(5915..6502)
                     /aSDomain="TIGR04536"
                     /aSTool="tigrfam"
                     /description="geobac_encap: encapsulated protein"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg5_207_0001"
                     /evalue="6.40E-90"
                     /identifier="TIGR04536"
                     /label="ctg5_207"
                     /locus_tag="ctg5_207"
                     /protein_end="197"
                     /protein_start="1"
                     /score="298.2"
                     /tool="antismash"
                     /translation="QEMSQLHAIFSRTRGYIDRFAEIIQPIIDAAQDEHTRLYYHHILE
                     EEEQRLGRLQELIPYIGSLDEAKKAEQLSDRELSRLLSDINLERFGLHNFREHLELSLY
                     EFKDEETRQLLNSMRESTQEDYLRVKEIMAALSERFSDVHRPDLTDHDEGYDIHQVDHL
                     KASSSPAVANTGKTAAAPAAKTGRKGLTVGSLK"
     CDS             6748..7029
                     /locus_tag="ctg5_208"
                     /transl_table=11
                     /translation="MSSILVIGGDRLGNIVDFLEEQGFTNIHHVTGRKNSQTGVKIPTG
                     IHMILVLTDFVNHNLAKTVKSQAKDRELPILFCKRSCSAIAKALVQGA"
     PFAM_domain     6754..7017
                     /aSDomain="DUF2325"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF10087.11"
                     /description="Uncharacterized protein conserved in bacteria
                     (DUF2325)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_208_0004"
                     /evalue="3.10E-18"
                     /label="ctg5_208"
                     /locus_tag="ctg5_208"
                     /protein_end="90"
                     /protein_start="2"
                     /score="65.8"
                     /tool="antismash"
                     /translation="SILVIGGDRLGNIVDFLEEQGFTNIHHVTGRKNSQTGVKIPTGIH
                     MILVLTDFVNHNLAKTVKSQAKDRELPILFCKRSCSAIAKALV"
     CDS             7093..7239
                     /locus_tag="ctg5_209"
                     /transl_table=11
                     /translation="VKQNKLDELPIAKLSEQQLQHLRAAEEEITKEGTDVYLIAFEKQA
                     PSQ"
     CDS             complement(7320..7607)
                     /locus_tag="ctg5_210"
                     /transl_table=11
                     /translation="MFAPTGTDGSPLGLENSVTAGIISAKNRRLHVAKRMYDEIFQTDA
                     AINPGNSGGPLINLNGEVVGLNAFIIQSSQCLGFAIGIDALKQQLDQFVF"
     PFAM_domain     complement(7359..7559)
                     /aSDomain="Trypsin"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00089.28"
                     /description="Trypsin"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_210_0005"
                     /evalue="6.10E-08"
                     /label="ctg5_210"
                     /locus_tag="ctg5_210"
                     /protein_end="83"
                     /protein_start="16"
                     /score="32.8"
                     /tool="antismash"
                     /translation="SVTAGIISAKNRRLHVAKRMYDEIFQTDAAINPGNSGGPLINLNG
                     EVVGLNAFIIQSSQCLGFAIGI"
     CDS             complement(7616..9064)
                     /locus_tag="ctg5_211"
                     /transl_table=11
                     /translation="MRTAVYIRVSTEEQASDGFSMAAQKERLTAYISSQGWTLSTIYAD
                     EGFSAKDTKRPALQRMLGDIRSGSIDVVLVYRLDRLTRSVLDLYRLLQDFEKYGVRFKS
                     CTEVYDTTTAIGRLFITLVAALAQWERENLGERVKLGMEQMARERKRPGGPPPYGYMLH
                     HHQLIPHPEEAKVVSRMYQQYLSGYTPGQIAEKANRDGHLGKHGGKWSGSTVTRLLRNP
                     VYYGALRWNYAEAGGQQNKRDEWILVEDAHPAIVDRETFLKVQERMGLRQRTHPRVLSS
                     PFVFSGLLYCARCKAEMRGKTADIRKGGGKRYRHIYYLCKNRANGICQAPALREDRLED
                     EVLGQLSSAQEEMGSVFRRIVQERCRSSTAAADDRKQLTKKRQRWMKAYEAGVITLEEL
                     AARQQDLRRLEQEMSSGGNEAFFAPGAIDWRQWTDWKWIWQQAAREERRHLALLLLERV
                     EAEAWTTQGKKKQRTVKVRAIFYR"
     PFAM_domain     complement(8027..8212)
                     /aSDomain="Zn_ribbon_recom"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13408.8"
                     /description="Recombinase zinc beta ribbon domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_211_0008"
                     /evalue="5.60E-12"
                     /label="ctg5_211"
                     /locus_tag="ctg5_211"
                     /protein_end="346"
                     /protein_start="284"
                     /score="46.1"
                     /tool="antismash"
                     /translation="FSGLLYCARCKAEMRGKTADIRKGGGKRYRHIYYLCKNRANGICQ
                     APALREDRLEDEVLGQL"
     PFAM_domain     complement(8261..8554)
                     /aSDomain="Recombinase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07508.15"
                     /description="Recombinase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_211_0007"
                     /evalue="2.80E-27"
                     /label="ctg5_211"
                     /locus_tag="ctg5_211"
                     /protein_end="268"
                     /protein_start="170"
                     /score="95.2"
                     /tool="antismash"
                     /translation="PEEAKVVSRMYQQYLSGYTPGQIAEKANRDGHLGKHGGKWSGSTV
                     TRLLRNPVYYGALRWNYAEAGGQQNKRDEWILVEDAHPAIVDRETFLKVQERM"
     PFAM_domain     complement(8618..9055)
                     /aSDomain="Resolvase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00239.23"
                     /description="Resolvase, N terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_211_0006"
                     /evalue="3.00E-42"
                     /label="ctg5_211"
                     /locus_tag="ctg5_211"
                     /protein_end="149"
                     /protein_start="3"
                     /score="144.2"
                     /tool="antismash"
                     /translation="AVYIRVSTEEQASDGFSMAAQKERLTAYISSQGWTLSTIYADEGF
                     SAKDTKRPALQRMLGDIRSGSIDVVLVYRLDRLTRSVLDLYRLLQDFEKYGVRFKSCTE
                     VYDTTTAIGRLFITLVAALAQWERENLGERVKLGMEQMARER"
     CDS             complement(9217..9561)
                     /gene_functions="regulatory (smcogs)
                     SMCOG1167:transcriptional regulator (Score: 112.5; E-value:
                     9.2e-35)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg5_212"
                     /transl_table=11
                     /translation="MYHRGDTIVGQEFGNEQTCPKYEGAINILGKRWTGLIINVLLRGA
                     VRFKDIREMVPHMSDKMLSERLKELEEQGILERKVYPEIPVRIEYELTDKGKDLRPVID
                     SIHEWGQKWM"
     PFAM_domain     complement(9220..9480)
                     /aSDomain="HxlR"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01638.19"
                     /description="HxlR-like helix-turn-helix"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg5_212_0009"
                     /evalue="6.10E-33"
                     /label="ctg5_212"
                     /locus_tag="ctg5_212"
                     /protein_end="114"
                     /protein_start="27"
                     /score="112.6"
                     /tool="antismash"
                     /translation="ILGKRWTGLIINVLLRGAVRFKDIREMVPHMSDKMLSERLKELEE
                     QGILERKVYPEIPVRIEYELTDKGKDLRPVIDSIHEWGQKWM"
ORIGIN
        1 gcgtacctga tcggcagtct gacaggcggg gttctctttg accgctgggg gaaaatgcgc
       61 acagtatacg ccgcgacagc gggagcggta gccgtagctg tggcactggg cttcacaacc
      121 agctttcata cgtatacggt cttgctgctt ttgaacggat ttttctccgg catcatgttt
      181 cctgtgctca atgccctgat cggcctcttg tggcctgacg gcggccgcaa gggcttgaat
      241 acggtttatg tggcactcaa tatcggggta gcgatcggtt ccgcctcggc gggaatgctg
      301 gcgagcattt cctttgcctg gactttcttt ggaaatgcgg cgctgcagct ggcagttctg
      361 tttctcttta tctttttgag ccgcacggtc gatcttgagc gcggtcagga aaaagaaggc
      421 cggctcgtgg cagcgtcggc gaagcacaca gaggaaggtg agggggcaca gctgcctgcc
      481 gcgggattgg cggagaaggc cggcggatca aaaggggccg cggcggcaat ggcgacggat
      541 gccgctcaag ctgccggtcg cccgatggca tggctagcgc tcggtctgtt gtcggcaggg
      601 ttgttggtct gctggattat ttactcgcag tggacgactg tgctgtcggc gtatatgcag
      661 tcgctgggca tcacgctcag ggagtacagc ctgctgtgga cgctgaatgg tgctttgatc
      721 ctgttcgggc agccgtttat ctcctgggtg atcaggcact tcgcccgtac gctgaaagcg
      781 cagctgctgt taggcgccta cgtcttttcg ctctccatgc tgatcttgtc ccagacgacg
      841 gtgtacgccg gttttttcgt ggccatgttt gtcattacac tgggtgaaat gctggtgtgg
      901 cctgccgtcc cgaccatcgc tgccgagttg tctccaccgg gacgtgaagg gttcattcaa
      961 ggcctcatcg ggggcgtagg ctcaggagga cgcatggtcg gcccgctgat gggcgccgtg
     1021 atctttgaag cgctcggagc aaatgggatg gtctacttca tggctgcttt ggctttggtc
     1081 gctgtcgctt gctttaccgt gtacacgtcc tttgtaaagc gtcctgagat gaaggcagct
     1141 ctgcctgctc cggagaagct cgggtcatga caagcaatgc ttggacggat actcgtagct
     1201 gtcaggcgaa gaccagcagg ctttcagccg aggcctctca ggctgtcagt cgaggcccct
     1261 cagcctgtca gtgaggctcc tcagtttgtc agttgaggac tgccaggctg tctaacgagg
     1321 ccctgcaggc tgccgggcca gcaacagcaa agcagcccgg gccatggttc gggatattgt
     1381 attgacactc ccaacctgct caagtagtat agtagacatt caaagtaaat aggttgctgt
     1441 taacttatcc agagaggtgg agggactggc ccgatgaagc ccggcagcgg atcttgcagg
     1501 atattctgca gatgctgtgc caattccagc aggagcgatc ctgacagata agaacgtgga
     1561 gacgcacgat ccgccccttt ttctgttcag gaaaaggggc tttttgcatg cctgcttcga
     1621 tgtctggatt atttttgcga aagaaaggat agaaagccaa tgcgcatcca accatctgcc
     1681 atggtggacc gactgccgac gcagtttttt gccacgctgg tagcgaaaac aaacaaggcc
     1741 atcgcggccg gacatgacat tatcaatctt ggacagggca atcccgatct cccgacaccg
     1801 cagcatatcg tggaagagct gagcgagcag gccaagcgcc ctgtccatca caaatacccg
     1861 ccgttcagcg gcaggctgga gctgaagcag gccgtcgccc actggtacaa ggaagagttc
     1921 ggcgtggagc tggacgcgga ggaagaggta gccatcctgt ttggcagcaa gacaggtctg
     1981 gttgagattt gtcaggtgct gctgaacccc ggggacgtcg ctttggtgcc cgatccaggt
     2041 tatccggatt actggtccgg cgtggctgta gctgacggcc gcatggtcat gatgccgctc
     2101 aaggctgaaa acgcgtttct tccggattac ggccagttga agccggaaga tttggaaaaa
     2161 gccaagctga tgttcctcaa ttacccgaac aatccgacgg cggtcaacgc tccggtggaa
     2221 ttttttgaag agaccatccg cttcgcccgc aagcacgaga tcgtcgtctg ccatgatttc
     2281 gcctatggtg cgatcagctt tgacggcaaa aagccggtca gcttcatgca ggtgccgggg
     2341 gcaaaagaag taggagtgga gttttacacc ctgtcgaaaa cgtacaacat ggccggctgg
     2401 cgcgtcgggg ctatggtcgg aaacaaggag ctcgtacggc tggtcaattt gattcaggac
     2461 cattactttg tcagtctctt cggcgctgtg cagatggcgg cggcaaaagc gatgaccggc
     2521 tcgcagcaat gcgtacatga tctcgtctcc gtctaccaaa gcaggcggga tgtgctgttc
     2581 gccgagctcc accggatcgg ttggcaggcc aagccttcag agggctcgtt tttcagctgg
     2641 ctgcctgttc ccgaagggtt tacatcggtc gagttttccg atttgctgct ggaaaaggct
     2701 catgtggtgg ttgcgcctgg cgtcggtttt ggaccgacgg gcgaggggta tgtgcgcgcg
     2761 gcgcttcttt ccagcgaaga aaggctggcg gaggcagtgc gaaggattga gaagctgggg
     2821 ctgtttgctc ggtaagcttg ttggcgggct tcgggttatc ttgctccttg tccaggggcc
     2881 gcggtgggga gaagcctgga aacaggcttc tcctgcgatt cggggtggca caacaggctg
     2941 aatcaagcag gatactcacg gataaagcag gcttgattcg aaatgatact cacggatgcc
     3001 aaaacataat gacggcaatc ttttcaagaa agacagcctt atttttcgtg aatagcggaa
     3061 gccatacttg caacataaag catggcttcc caagcaaaag gcccggagct atgatcgctc
     3121 agggccttta tgatggcttg atggaatcag gtgatacccc aggccagctt tatggactag
     3181 gcgggatcgg cggttgccag ttcaagtcta tgtaccgtgt ttcgatggtt tgcaaagcga
     3241 gcttcagctt ttgcggaggc tgctccattc ctttgacaat cagggtttgg accaatacga
     3301 ctcccttgtc tgaattctcc atatacgtct tggcaaaatc ggtccgaacg tcgaaacgct
     3361 tgccgttttc gtcatacaga ttccagtaag ggaagctctc cagttttccc aagcccttca
     3421 actcgattat ccagacgggc tcgaatttgc ctacaaaaac ccgtcccacc gtacttttgc
     3481 tttttctcct gtttcttacc tctacagccc ggtttaccac caaaagaaaa gcaggaaaca
     3541 tctgcacaag catcgatacc cccaaccaaa gtagtcgcga gtaaaatccg acgatttcga
     3601 ccgagccatc gctcgacata gctaaaacct attccgccga tagatttgtg agattggact
     3661 tatgacggcc gcagtgataa aacaggtata agcgaaatct agggggaacc gaacatggaa
     3721 tattcatttt ccaaatcaac cagcgcactc agctcctcgg ccgtgcgcga aatcctgaag
     3781 ctgacacagg gcaatcaagt attgtctttg gcaggcggcc tgccggcaga ggattctttt
     3841 ccaatcgaag aaatgagaga agccttcaat cgcgctctgg atcacggagc aaaatcgctg
     3901 caatacggct tgactgacgg gtacattccg cttcgggaat gggtttcgtc ccgcatgaaa
     3961 caaaagcata tgaatgttgg cgtagaaaac atgctgatca ctaccggctc ccagcaggcc
     4021 gtcgacttgc tctgccgtgt ctatctggac gaaggcgacg tcgtgctggt agaaaatcca
     4081 acgtatctcg cggccgtgca actgttccaa tttcgcggca ttcgagctat tccggtggat
     4141 ggagatgcgg aaggcatgga tatggaggac cttgccagaa aaatcgcaca atatcgcccg
     4201 aagatggcct atgtcattcc gacgtttgca aacccgacag gaaaggtttg gagtctggaa
     4261 aaacgcttgg gactgctgaa acaatgccgg gaaaacaaca ttttgattct ggaagacgat
     4321 ccgtacgggg aaatccagtt cgacggcgag gcgccttatc gcagcatttt ttctctcgat
     4381 gagcatccgg acggatcggc tgtcgtctac accagcacct tctccaaaat cgtcgctccg
     4441 ggtcttcgaa cggggtgggc gattggagac agccaggtga ttcagatgat ggtaaaagcc
     4501 aagcaggcaa tcgacttgca gtccagcaca atcgaccaat ttgctctgta tgagctgctg
     4561 tcccggtttg atttggaaag ccatattcaa aaaatccgcg actcgtacaa agagcggatg
     4621 gagtggatgc acgagctgct ctgtcgtcaa accagcggca tgaagtggaa taaaccgaga
     4681 ggcggaatgt tcctctgggt agagctgccg gagcatgtac actccgagaa gctgctggcg
     4741 caggctgtca aggaaggcgt cgcgtttgtt cccggtatcc cgttctacgc ggaccagcct
     4801 caaacgaata cgatgcgcct caactatacg ctgctggatc gggaacatac cgaactggcg
     4861 atcaaccgct tgggcaaagc gtttgcggag tacagccagc tagtcgctca ctagcgataa
     4921 atgaatacgt ggatcgaata aaacagggtg tcccgaagtc tttgacgacg ggggcaccct
     4981 gttttttgca gcctcggtta ttattcttct gtttcctcca acgtgcagat cgcactcggc
     5041 cgcttgatgc ggagcacgac acactcatag acgcggaaca ggctgttcat ttgctcgtct
     5101 ccgaggaagg acgtatcgaa gtcttccgca atagccaggt caaggttgtg acggccggtt
     5161 gcgaccagga cgccggatcg gcccttgatg gtcggggatt ggaagacgcc gtcggtgacc
     5221 agattgcgga cgtgctcgat ttccagtaca ttcgttccct tgtgtacgcg gtgcagaagc
     5281 gagtacagct caggggagac gaccaatgca tatggaccgc tgtgtcccat cttcaacagc
     5341 ttgttgcgcg cttccacgat gtcggcaaag gcattgcccg actccatcca gtcgctcttc
     5401 aagtgggtga gacgcccctt cacattcatc aagcccggca ggtccagacc cggggagccg
     5461 ttgaagatca ggtcatcctc catgagagcg cagccggctg ccgcattcgc agcggcactc
     5521 atatccagcg gcatacccaa tgtccgggct tgggccacat cccgccagta cagcatgaag
     5581 tctttgtaca ggatgggaat ggtcaggctg acccgtttgc tgggctgggt catttccagc
     5641 gattcgccgc gcaggttcaa tccgccaaag cgggactcgt cgtaaatatc gttggtaatc
     5701 gtctgaatgc cctcaccgag cgggccgtaa atatcgagaa agcggcggcc aaccagctgg
     5761 cgtcgcgcca tatcaacaac ggtttcatcc agttgcttcc attcatcgtg agtcaaaggg
     5821 gaatcgggat atttgcggag cttgtccatt ccacatacct ccaaatcgtt tctgtttacc
     5881 tgccggaatc tgcaagcaaa gtttaaaaca tgccttttaa gctgcctaca gtcaggccct
     5941 ttctgcctgt tttggctgct ggggcggcag cagtcttgcc ggtattggca actgccggag
     6001 aagaggaggc tttcagatga tccacctggt ggatatcgta tccctcgtca tgatcggtca
     6061 ggtcaggccg gtgcacatcc gagaaacgct cgcttagcgc tgccatgatt tcctttacgc
     6121 ggaggtagtc ctcctgggtg gattcgcgca tgctgtttag gagctggcgg gtttcctcgt
     6181 ctttgaactc atacaaagat agctccagat gctcgcgaaa attgtgcaag ccgaaccgtt
     6241 cgagattgat gtcggataaa aggcgggaca gttcgcggtc gctgagctgt tccgcttttt
     6301 ttgcctcgtc gagcgagcct atgtagggaa tcagctcctg caaacggccg aggcgctgtt
     6361 cttcttcctc caggatgtgg tggtagtaga gtcgcgtatg ctcgtcctgt gcagcatcga
     6421 taatgggctg gataatctcc gcaaagcggt cgatatagcc cctggttcga gaaaatatgg
     6481 cgtgcagttg tgacatttct tgcacccgac gccacctcct tgcaggtgat ttgtttcatt
     6541 ataacgttgg aaaaataaac cgggcaacct ttatctgcat gtatctccta gtttgtgtca
     6601 gtctgaaaaa cttatcatgg agagtattgt caaagctaaa tggcaatgat aatatttatc
     6661 aataagagtg atattcatta tcaattagaa atgaaagcca tagatataca caacaaaagt
     6721 cgataaacca ggttaggagg aaaaaacatg tcatctattc tcgtcattgg cggagaccgc
     6781 ctcggaaata ttgtagactt tttggaggag cagggtttta ccaatattca tcatgtgacg
     6841 gggcggaaaa actcccagac cggcgtaaaa atccctactg gcattcacat gattttggtg
     6901 ctgacggatt tcgtcaacca caatctggcc aagaccgtaa aaagtcaggc aaaggacagg
     6961 gagctgccga ttctcttttg caaacgttcc tgctcggcga tcgcgaaggc tttggttcag
     7021 ggagcataaa tacatacccc ccgctgtgga aagactacag actgatatgt acaccaaacg
     7081 ggggaatgaa aggtgaagca aaacaagctt gacgaattgc cgattgccaa actgtccgag
     7141 cagcagcttc agcatctgcg agcagcggaa gaagagatca ccaaagaagg cacagatgtc
     7201 tacctgatcg cttttgaaaa acaggccccg tcccagtgac ggggttttgc tttgaaattg
     7261 gctccgccag gccgagggct ccgacggaac ggagccgggg acttggcggg acctttcctc
     7321 taaaacacaa attgatccaa ctgctgtttc aatgcatcga ttccgatggc aaatccaagg
     7381 cattggctgg actggatgat aaacgcgttt agccctacga cttctccatt cagattgatc
     7441 aagggacctc cactgttgcc gggattgatg gcggcatcgg tctggaaaat ttcgtcatac
     7501 attcgtttgg cgacatggag acggcgattt tttgcactga tgatgcccgc tgtgacggag
     7561 ttttccaaac cgagcggtga accgtctgtt cctgtaggag caaacatctt tgccgttacc
     7621 ggtaaaaaat ggcccgcact ttcaccgtac gctgcttctt ttttccttgg gtcgtccaag
     7681 cctctgcctc cactcgctcc aacaacagca gggccaaatg ccggcgttct tcacgcgcag
     7741 cctgctgcca tatccatttc caatctgtcc attgccgcca gtcgattgct ccgggagcaa
     7801 agaaagcctc atttccaccc gaagacatct cctgctccaa ccgcctcaag tcctgctgcc
     7861 ttgctgcgag ctcctccagc gtaatcacgc ctgcctcata cgctttcatc cagcgctgcc
     7921 gttttttggt aagctgcttg cgatcatcgg cagccgctgt agaggacctg cacctctcct
     7981 gaacgatgcg gcgaaagacc gagcccattt cctcttgtgc agaagaaagc tgcccaagca
     8041 cctcgtcctc cagccggtct tcccggagtg caggcgcctg acaaatgccg ttggctctgt
     8101 ttttgcacag gtaatagatg tgacgatacc gctttccgcc gcctttgcgg atatctgctg
     8161 ttttcccgcg catctcggct ttgcagcggg cacagtataa caagccggaa aaaacaaacg
     8221 gagaggaaag gactctcgga tgcgttctct gccgcagccc catccgttcc tgtaccttga
     8281 ggaacgtttc ccgatcgaca atcgccggat gggcgtcttc aaccagaatc cattcgtccc
     8341 gcttgttctg ctgtccgcca gcttccgcgt agttccagcg aagcgctccg tagtacaccg
     8401 gattgcgcag caggcgagtc accgtgctgc cgctccattt tccgccatgc ttgcccaaat
     8461 gtccgtcccg attggctttc tctgcaatct ggccaggcgt ataaccggaa aggtattgct
     8521 gatacatgcg gctgaccact ttggcttcct cgggatgggg tatgagctga tggtgatgca
     8581 gcatgtagcc gtagggagga ggaccgcccg ggcgttttcg ttcccgcgcc atttgctcca
     8641 tgcccaattt gacccgctct ccgaggtttt cccgctccca ctgggccagg gcggctacca
     8701 gtgtgataaa cagtctgccg atggctgttg tcgtatcgta cacctctgta cagcttttaa
     8761 agcggacacc gtatttctca aaatcctgaa gcaaccggta caagtcgaga acagaccggg
     8821 tgagccgatc cagccggtag accaggacga catcgatgct tccggaacga atgtcgccga
     8881 gcattcgctg cagggccggc cttttcgtat ccttggcgct gaagccctcg tcagcataga
     8941 tggtggaaag cgtccagccc tgcgagctga tgtaggcagt cagccgttct ttctgagcag
     9001 ccatggaaaa cccgtcggaa gcctgttctt ccgtactgac gcgaatgtac acagctgtcc
     9061 tcatggcatc aacctcccgg ataacggatt gctagagttt acaagcaggc cgggtttcct
     9121 atgcctggac agctgaaagt ttttgccggg gaaaataaaa agcccgctgc gattcgtgac
     9181 gaaaacgcag caggcgagag ggatctgttg gggaggctac atccactttt gcccccattc
     9241 gtgaatggag tcgatcactg gccgaagatc cttccccttg tcggtcagct cgtactcgat
     9301 acggacgggt atttccggat acactttgcg ttccagaatg ccctgctcct ccaattcctt
     9361 caatcgctcg gaaagcattt tatcgctcat gtgcggaacc atttcccgga tatccttgaa
     9421 tcggacagcc ccgcgcaaca gcacgttgat aatcaaaccg gtccatctct taccgagaat
     9481 attgatagct ccttcgtatt tggggcacgt ctgttcattc ccaaattctt gacccacgat
     9541 ggtgtcacct ctgtggtaca tcttgttttc ctctttattg tagcatatca acttgaataa
     9601 agtaagtaac acaatgagac ttggtgccta tattgttttg tccagagtgt ccggacttat
     9661 gcaagcgctg gcgataaaaa gggcgatggc ccgtttccag cccatgggtc tccacttttt
     9721 tcatttcaac atagactatt cgagaacata gacggagggt gggtcttgat gtcaaaaaaa
     9781 ccggtaatcg gtattcttac gtggagagaa gggaaacggt ttggcgagcc ggggtacttg
     9841 cgaaggctgg tgacggcagg aaagcgaatg ggggcagaga cgttcctgtt ttcccatcag
     9901 gatgtgaata cggctgagaa aaagatcagg ggattcgttc cggcaaaggg aggcgggtgg
     9961 gaaagccgcc tgtttccgtg gccagaggtc gtcattgacc ggtaccgaaa aagggaagac
    10021 gcgtacatgc ggctgcgtca tggcggggtg tttcagtttg ccaacagtcc gtttggaaag
    10081 aaatggaggg tgactgagct tttggccaaa gacgatcggg tcaaaagatg gattcccgag
    10141 acgtacgtgt acgcgaaagg aaaagtaaag gcgatgctgg accgctatcc gatcgtctat
    10201 gtaaaaccgg gcaatgggac agggggacgc agcatcgtca agatctcgca ggcagggaaa
    10261 cacttcctca ttcaggggag agatcgccgg ctgcaaagac ggaaggctag ctttgcgacc
    10321 agggatggcg tggagagctg gattcaaaag tgggtgcggg agcagcggat tcgagacggg
    10381 aactttctcg tgcagcaggg gctcgatctt tcgctggtgc cgggccgcgt cgctgatgta
    10441 cgccttttga tccaaaaaaa cagcgctgga gagtgggagg tgactggctc tggagtacgc
    10501 ctcggggaga cgggcagctc catttccaat ctgcacggag gggggaaggc catttcattc
    10561 cagtcgttcg tgggcaagcg gttcggtccg gagcggacaa agcttatcca gcaggagtgc
    10621 caccggatgg cccacgagat agcgtctgtc ctggaggaaa aatttgggcg gatgatggag
    10681 tttggtctgg acgtaggggt ggatgtagcc ggaagagtct ggctgatcga agtaaacccc
    10741 aagccgggcc gggaaatctt caggcagatg ggggatacgc gcacgtatga agaagcgatc
    10801 gcccgccccg tctcctttgc gcttcatctg atcaactccg gacaggtgc
//
