LOCUS       c00033_NZ_JAMD..       11697 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JAMDNA010000033.1 Paenibacillus alvei strain NRRL B-04300
            contig_0000033, whole genome shotgun sequence.
ACCESSION   c00033_NZ_JAMD..
VERSION     c00033_NZ_JAMD..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 16:57:11
            Original ID  :: NZ_JAMDNA010000033.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 40211
            Orig. end    :: 51908
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11697
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[50211:50736](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10525)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     cand_cluster    1..11697
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11697
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     CDS             complement(866..1486)
                     /locus_tag="ctg33_42"
                     /transl_table=11
                     /translation="VVRDIERLPKRQRQQKLAAIIEENPFVTDQELTRKLKVSIQTIRL
                     DRMELGIPELRERLKLMAELSYDQVKSLPLHEVIGDIVDLQLDRSGISIFEIREEHVFS
                     RTHIARGHHLFAQANSLAVAVINDEIALTASADIRFLRSVKLGEKCIAKAYVRSISGER
                     SKAKVEVFSYVGEEMVFQGNFVIYRSAGTVISSEGGELHENRH"
     PFAM_domain     complement(962..1162)
                     /aSDomain="4HBT"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03061.24"
                     /description="Thioesterase superfamily"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_42_0001"
                     /evalue="1.10E-04"
                     /label="ctg33_42"
                     /locus_tag="ctg33_42"
                     /protein_end="175"
                     /protein_start="108"
                     /score="22.6"
                     /tool="antismash"
                     /translation="ARGHHLFAQANSLAVAVINDEIALTASADIRFLRSVKLGEKCIAK
                     AYVRSISGERSKAKVEVFSYVG"
     CDS             complement(1979..2494)
                     /locus_tag="ctg33_43"
                     /transl_table=11
                     /translation="MEFRFRDIVAGRGETQIDTAFRADEVARERSDVTISEPVHVELSV
                     KAESDGLARAHGKLTAKMELQCSRCLTSFTETYVIPFEEQFKLKDSTDLPAEEEEEDVI
                     LVADDLVDLKPYVEETLLMNLPYAPLCDEACKGLCPTCGTNRNEHPCGCSKERIDPRLA
                     GLQDFFKK"
     PFAM_domain     complement(1991..2329)
                     /aSDomain="YceD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02620.19"
                     /description="Large ribosomal RNA subunit accumulation
                     protein YceD"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_43_0002"
                     /evalue="1.10E-30"
                     /label="ctg33_43"
                     /locus_tag="ctg33_43"
                     /protein_end="168"
                     /protein_start="55"
                     /score="106.3"
                     /tool="antismash"
                     /translation="HGKLTAKMELQCSRCLTSFTETYVIPFEEQFKLKDSTDLPAEEEE
                     EDVILVADDLVDLKPYVEETLLMNLPYAPLCDEACKGLCPTCGTNRNEHPCGCSKERID
                     PRLAGLQDF"
     PFAM_domain     2753..3970
                     /aSDomain="HIGH_NTase1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF05636.13"
                     /description="HIGH Nucleotidyl Transferase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_44_0003"
                     /evalue="7.00E-132"
                     /label="ctg33_44"
                     /locus_tag="ctg33_44"
                     /protein_end="406"
                     /protein_start="0"
                     /score="440.6"
                     /tool="antismash"
                     /translation="MRTLGIVVEYNPMHNGHVYHLQQSKKISNCDAVVAVMSGHFLQRG
                     EPALSDKWSRTDMALANGVDLVLELPVAYAVQPAEWFAYGAVATLQATGVIDALCFGSE
                     LGDAEPFIQAADVLVNEPSGFTDQLQEHLKAGLTYPAAYAAAAGHAIKNSATLPSTGQA
                     DGWMIQPNNSLGLHYVMALRRLNSTIEAYTIARTQAGYHDTNAPEGSIASATAVRKLWV
                     ENKNGSLGACAQYLPTSTLQILEREHRLGRAPISWDSYTRELIYRLHQLSAKSLARHLE
                     VTEGLEHRVKQALGRMADYRVESLLEQLKTKRYTRTKLQRMLTHIILHHDKFHFGPDSL
                     EKGPWYIRVLGFTDKGRALLKRMKKSAELPIVSTANRNNIGLGGVCGLEADIRSTSLYA
                     NAFQQWN"
     CDS             2753..4021
                     /locus_tag="ctg33_44"
                     /transl_table=11
                     /translation="MRTLGIVVEYNPMHNGHVYHLQQSKKISNCDAVVAVMSGHFLQRG
                     EPALSDKWSRTDMALANGVDLVLELPVAYAVQPAEWFAYGAVATLQATGVIDALCFGSE
                     LGDAEPFIQAADVLVNEPSGFTDQLQEHLKAGLTYPAAYAAAAGHAIKNSATLPSTGQA
                     DGWMIQPNNSLGLHYVMALRRLNSTIEAYTIARTQAGYHDTNAPEGSIASATAVRKLWV
                     ENKNGSLGACAQYLPTSTLQILEREHRLGRAPISWDSYTRELIYRLHQLSAKSLARHLE
                     VTEGLEHRVKQALGRMADYRVESLLEQLKTKRYTRTKLQRMLTHIILHHDKFHFGPDSL
                     EKGPWYIRVLGFTDKGRALLKRMKKSAELPIVSTANRNNIGLGGVCGLEADIRSTSLYA
                     NAFQQWNARDALRDYYEPPRRNS"
     CDS             complement(4108..5181)
                     /locus_tag="ctg33_45"
                     /transl_table=11
                     /translation="MKEDELLGTQKTKRWRLGNWKWFFAALLFLYIMVYMPTPYVIYTP
                     GSAEQVKPMVSVQAGDETEDGTFMLTTVRRTYANLALLIWKSFDPHAEFGKKADSLQGR
                     SEQEYVTEQLFNMSDSQLGAVLAAYNQLHIPYQLKSEGVFVIYNYPNLAHNEFETNDRI
                     VEVDGQPVVDFDSLQAVMKGRKAGETVQVKVERNKQQKVVKATLVELSDPNKKEEKRVG
                     FGLRYGQRKEAAPDDKGKTVTFKDSEIGGPSAGLMFTLELINRLTPGDLTQGHRIAGTG
                     TIDPDGKVGIIGGVQFKVVAADREQAELFLVPEGNYAEAKAKLESMDTKMKLVPVRTVG
                     EALQAIKQFSSKQNAGK"
     PFAM_domain     complement(4147..4431)
                     /aSDomain="Lon_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF05362.15"
                     /description="Lon protease (S16) C-terminal proteolytic
                     domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_45_0005"
                     /evalue="4.40E-10"
                     /label="ctg33_45"
                     /locus_tag="ctg33_45"
                     /protein_end="345"
                     /protein_start="250"
                     /score="39.5"
                     /tool="antismash"
                     /translation="GPSAGLMFTLELINRLTPGDLTQGHRIAGTGTIDPDGKVGIIGGV
                     QFKVVAADREQAELFLVPEGNYAEAKAKLESMDTKMKLVPVRTVGEALQA"
     PFAM_domain     complement(4564..4743)
                     /aSDomain="PDZ_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13180.8"
                     /description="PDZ domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_45_0004"
                     /evalue="7.20E-09"
                     /label="ctg33_45"
                     /locus_tag="ctg33_45"
                     /protein_end="206"
                     /protein_start="146"
                     /score="36.0"
                     /tool="antismash"
                     /translation="YNYPNLAHNEFETNDRIVEVDGQPVVDFDSLQAVMKGRKAGETVQ
                     VKVERNKQQKVVKAT"
     CDS             5307..6575
                     /locus_tag="ctg33_46"
                     /transl_table=11
                     /translation="MFSFRTPLSQHPFMTLLMGSTAVLFVICIVSYPDQAFQASLQGLK
                     IWWTIIFPALLPFLVLSEMLYAYGFVHGLGVMLDPIMRLLFRLPGVGGWAWSVGWTAGY
                     PAGAKAVAQLRKQQTLSRQEAERLLCLSHANSPIFMIAVVGVGFMQQPHLGLVIAIVHW
                     LSAILTTLIWRTLHRMHPELQAVRPNHNITKAHRNFYRRLISEMEDAHRQDGRPFGRML
                     GESVSSAVQTLMMVGGYMMIFSVIIQVLRIAIPQEFGKHILNGLFEVNLAAYTLGSTAF
                     SSPVFQAALLSAVIAWSGISALLQVHSIIKETDLRFSHFLISRLLHALCAFLLTYVLWS
                     PLHAWLSTPNSAMSTLSPFAASHTDVEHVSSILDSSGSLPFITYMSQLPIWAQASILIP
                     VACLFLFALFVLAQTIGRLFTHR"
     CDS             complement(6629..7144)
                     /locus_tag="ctg33_47"
                     /transl_table=11
                     /translation="MTEHTERVHEPRIAVYPGSFDPVTLGHLDIIQRAAKQFDKLIVAV
                     LNNLSKQPTFSVEERKELLRQITRHIPNVEIDSFRDLLVNYMEQKQGHVIVRGIRSVTD
                     FEYELQMASTNQKLNPRVETIFMMTNPKYSYLSSSMVKEIAKFNGDVSELVAPEVELAL
                     RDKFKDTN"
     aSDomain        complement(6644..7108)
                     /aSDomain="TIGR01510"
                     /aSTool="tigrfam"
                     /description="coaD_prev_kdtB: pantetheine-phosphate
                     adenylyltransferase"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg33_47_0001"
                     /evalue="3.60E-63"
                     /identifier="TIGR01510"
                     /label="ctg33_47"
                     /locus_tag="ctg33_47"
                     /protein_end="167"
                     /protein_start="12"
                     /score="210.2"
                     /tool="antismash"
                     /translation="IAVYPGSFDPVTLGHLDIIQRAAKQFDKLIVAVLNNLSKQPTFSV
                     EERKELLRQITRHIPNVEIDSFRDLLVNYMEQKQGHVIVRGIRSVTDFEYELQMASTNQ
                     KLNPRVETIFMMTNPKYSYLSSSMVKEIAKFNGDVSELVAPEVELALRDKF"
     PFAM_domain     complement(6716..7102)
                     /aSDomain="CTP_transf_like"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01467.28"
                     /description="Cytidylyltransferase-like"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_47_0006"
                     /evalue="5.10E-23"
                     /label="ctg33_47"
                     /locus_tag="ctg33_47"
                     /protein_end="143"
                     /protein_start="14"
                     /score="82.0"
                     /tool="antismash"
                     /translation="VYPGSFDPVTLGHLDIIQRAAKQFDKLIVAVLNNLSKQPTFSVEE
                     RKELLRQITRHIPNVEIDSFRDLLVNYMEQKQGHVIVRGIRSVTDFEYELQMASTNQKL
                     NPRVETIFMMTNPKYSYLSSSMVKE"
     aSDomain        complement(6926..7108)
                     /aSDomain="TIGR00125"
                     /aSTool="tigrfam"
                     /description="cyt_tran_rel: cytidyltransferase-like domain"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg33_47_0002"
                     /evalue="8.40E-20"
                     /identifier="TIGR00125"
                     /label="ctg33_47"
                     /locus_tag="ctg33_47"
                     /protein_end="73"
                     /protein_start="12"
                     /score="68.7"
                     /tool="antismash"
                     /translation="IAVYPGSFDPVTLGHLDIIQRAAKQFDKLIVAVLNNLSKQPTFSV
                     EERKELLRQITRHIPN"
     CDS             complement(7213..7803)
                     /locus_tag="ctg33_48"
                     /transl_table=11
                     /translation="LRVISGTARGRSLKPVPGMGTRPTTDKVKEALFSMIGPYFDGGYV
                     LDLFAGTGGLGIEALSRGAGQGIFVDKDPKAVEVVKHNVQTAGVANRSEVYRNDAKRAL
                     KALAKRDIAFELIFLDPPYRLKDADELLEGMWNDGLISESAIIVVEHDSKHVYPNEIGP
                     LYVWKKAEYGEIGVTVYRVYGQTEKEAAQEQDA"
     aSDomain        complement(7264..7800)
                     /aSDomain="TIGR00095"
                     /aSTool="tigrfam"
                     /description="TIGR00095: 16S rRNA
                     (guanine(966)-N(2))-methyltransferase RsmD"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg33_48_0003"
                     /evalue="1.80E-52"
                     /identifier="TIGR00095"
                     /label="ctg33_48"
                     /locus_tag="ctg33_48"
                     /protein_end="180"
                     /protein_start="1"
                     /score="175.8"
                     /tool="antismash"
                     /translation="RVISGTARGRSLKPVPGMGTRPTTDKVKEALFSMIGPYFDGGYVL
                     DLFAGTGGLGIEALSRGAGQGIFVDKDPKAVEVVKHNVQTAGVANRSEVYRNDAKRALK
                     ALAKRDIAFELIFLDPPYRLKDADELLEGMWNDGLISESAIIVVEHDSKHVYPNEIGPL
                     YVWKKAEYGEIGVTVY"
     PFAM_domain     complement(7264..7803)
                     /aSDomain="Cons_hypoth95"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03602.17"
                     /description="Conserved hypothetical protein 95"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_48_0007"
                     /evalue="1.10E-55"
                     /label="ctg33_48"
                     /locus_tag="ctg33_48"
                     /protein_end="180"
                     /protein_start="0"
                     /score="188.3"
                     /tool="antismash"
                     /translation="LRVISGTARGRSLKPVPGMGTRPTTDKVKEALFSMIGPYFDGGYV
                     LDLFAGTGGLGIEALSRGAGQGIFVDKDPKAVEVVKHNVQTAGVANRSEVYRNDAKRAL
                     KALAKRDIAFELIFLDPPYRLKDADELLEGMWNDGLISESAIIVVEHDSKHVYPNEIGP
                     LYVWKKAEYGEIGVTVY"
     CDS             complement(8118..8702)
                     /locus_tag="ctg33_49"
                     /transl_table=11
                     /translation="MRHLIIYAHPNPNSFNHAIMEIVQAELEDQGHAALVRNLYEMEFN
                     PVLSAKDFELFGQHKLPSDIEEEQGHVAWADHMIMVYPIWWASMPAIMKGYIDRVFSYG
                     FAYRYGADGIEKLLKGKSALLLSTHGNSHEQYEQSGMYESMQQTTDNGIFEFVGMKVLA
                     HRFFASVTTIDEEARRQMLISVRELLQKLHK"
     PFAM_domain     complement(8148..8702)
                     /aSDomain="Flavodoxin_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02525.19"
                     /description="Flavodoxin-like fold"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_49_0008"
                     /evalue="2.40E-53"
                     /label="ctg33_49"
                     /locus_tag="ctg33_49"
                     /protein_end="185"
                     /protein_start="0"
                     /score="180.9"
                     /tool="antismash"
                     /translation="MRHLIIYAHPNPNSFNHAIMEIVQAELEDQGHAALVRNLYEMEFN
                     PVLSAKDFELFGQHKLPSDIEEEQGHVAWADHMIMVYPIWWASMPAIMKGYIDRVFSYG
                     FAYRYGADGIEKLLKGKSALLLSTHGNSHEQYEQSGMYESMQQTTDNGIFEFVGMKVLA
                     HRFFASVTTIDEEARRQMLISV"
     CDS             complement(9071..9295)
                     /locus_tag="ctg33_50"
                     /transl_table=11
                     /translation="MSKRSETKNNERTSNIQYRDEVKQQEREREKFYALFNIFVHEIKH
                     VNPELFPSCLEIEAMMNEAFEDIEKHNCI"
     CDS             complement(9499..9864)
                     /locus_tag="ctg33_51"
                     /transl_table=11
                     /translation="MEDNVEILGMRVIDDKVTTEYGFFKPKEIFFIDVFQPKSNYFVPR
                     FHTKKGEFTVFLTLESCKVTFKHLTKLDSGNLVDIDQITYVTESPFAITAYFPTGTKTA
                     NVSRVKRKYIEHLIKKT"
     CDS             complement(9876..10004)
                     /locus_tag="ctg33_52"
                     /transl_table=11
                     /translation="MKQQTARRIADFLLASAKLSARNKKTFIGAKELPAELKDSKK"
     CDS             complement(10001..10525)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg33_53"
                     /sec_met_domain="AgrB (E-value: 1.3e-21, bitscore: 68.6,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIERWANYMALRIKAADPEGQTSVEVMEYGLGVYLNFFVTVILSC
                     LGGWVTGEIINTLIAAAAFGILRYFSGGVHMKSLTACAIVSALIFTLIPHIELSASIVL
                     ILNTVAVGIFLICAPNTYEELARPLLSNTVSKILAVIIVLFNFIIVSPVLALVFMVQGI
                     LILPWKGGRKL"
     PFAM_domain     complement(10064..10519)
                     /aSDomain="AgrB"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04647.17"
                     /description="Accessory gene regulator B"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg33_53_0009"
                     /evalue="6.10E-19"
                     /label="ctg33_53"
                     /locus_tag="ctg33_53"
                     /protein_end="154"
                     /protein_start="2"
                     /score="68.2"
                     /tool="antismash"
                     /translation="ERWANYMALRIKAADPEGQTSVEVMEYGLGVYLNFFVTVILSCLG
                     GWVTGEIINTLIAAAAFGILRYFSGGVHMKSLTACAIVSALIFTLIPHIELSASIVLIL
                     NTVAVGIFLICAPNTYEELARPLLSNTVSKILAVIIVLFNFIIVSPVL"
     CDS             complement(10525..11196)
                     /locus_tag="ctg33_54"
                     /transl_table=11
                     /translation="LDSTSTSIYIFLGGMDAFAAFVLILKLYRLPLREYIKELGIMSVF
                     IAIVSYFMRIVLDVPVMDLPLQLLLFTLFIRYVMMVKLFYSGIITALGLGGYLLIQLLI
                     YIAFTSIGVNQQFALQSTGMTVNIFQITAIVSAYLIGYILKKFNLGFSFIIRPPHDFSV
                     KEDYSTSRNKALLFWSLATMVVLSISLTALFHMKIEYVIPLLLITFGALYMQSRRRDIE
                     D"
     CDS             complement(11520..11696)
                     /locus_tag="ctg33_55"
                     /transl_table=11
                     /translation="IVPWLFIVLIFTNAPVVRQCSWLCFCNRSTIMHCFLQPRYLVKID
                     VNYSKIIPVLNKR"
ORIGIN
        1 atgggcctta attacaagtc cattcaatcc tagcagcgga gctccgccat gttccttgta
       61 gtccatcgtc ttgcgcagac ccttaagtgc tggcatcaac atggccgccg ccagcttcgt
      121 ataccatgta cgcgtgaatt cccgcttcag cacaccaaat aatgagctgg ccgttccttc
      181 taacgatttc agcatgacat tgcctgcgaa tccgtcgcac acgagtacgt cacaggcgct
      241 ttccaatacg tctctggctt cgacattgcc tacgaattga atcggcagtt ccgcgaggag
      301 cggatatacc tgcttcgtca attcattccc cttcattgct tccgtcccca cgttcagcag
      361 accgacgcga ggtctcgaca tgccgtgcac cttttcccga tagatgctgc ccattaaagc
      421 atattgagca agatgctccg gctttgcatc catattggcg cccaagtcca gtgctagcac
      481 gcctcttccg tccatcgtcg ggatcatagg ggccaatgca ggtcgctcaa ccccattcat
      541 tcgtcctacg atcagcagac ctgttgtcat caacgcccct gtattgcctg cagatatcat
      601 acagtccgca tcaccttcac gaaccatgcg gcccgcaaca accattgagg cttctttctt
      661 acgacgaacc gctttaactg gctcatcatc agcctcaatc gtatctggtg catgaacgat
      721 gcgcacatta gacgtggaag aggtcatgta cggagcaagc ttcgcttcat ctcctattag
      781 cagcagctca acgtctgacc attccttagc tgcggccaat gcaccctcta cattgctttg
      841 cggcgcatta tctcctccca ttgcatcaat ggcgattctc atgcagttct cctccttcgc
      901 tgctaatcac cgttcctgcc gaacgataga tgacaaagtt cccttggaac accatttctt
      961 ctcccacata actgaacact tctaccttcg ccttgcttcg ttcgcctgag atggatcgca
     1021 catacgcctt cgcgatgcac ttctcgccca gtttcacgga acgaaggaat cgaatatctg
     1081 ctgatgccgt taatgcaatc tcatcattaa tgaccgcaac tgctagcgag ttcgcctgag
     1141 cgaacagatg atgtccgcgt gcaatatgcg ttctggagaa tacatgctct tcacggattt
     1201 caaatatcga tatcccgctg cgatccagct gcaaatctac aatatcacca atgacttcat
     1261 gcagtggcaa ggattttact tgatcatagg agagctctgc catcaacttc agtcgttccc
     1321 ttagttcagg aatcccaagc tccatccggt caagccggat cgtctggatg ctcaccttta
     1381 gcttgcgggt caattcctga tcggtcacga atggattctc ttcaatgatt gcagctagct
     1441 tctgctgacg ctgtctttta ggtaaacgct cgatgtcccg caccaccttt gctcgttgcc
     1501 catatgctag aggcttggca cgctgctcaa ctgcgaatcg tgctggcctt tatgtatatc
     1561 atagatcatt ttgaaccgtt ttggaaaact catttagaac ctggtactaa gagtagtata
     1621 tataaaaatc tcctcaaaga aaagcaccag ttttatacct tttctaattt tcctaatttc
     1681 atccaaatag tctaaacaaa aaaagtagca ctccgcgaga tcgcgaagta ctacttttcc
     1741 tcgtattggt atacgattat tgtttgataa tctctctagc tttgtatgtt ccgcacactt
     1801 tgcatacatg gtgcgccaat ttcaactcac cgcattgtgc gcactttacc atgccgggta
     1861 cggacaattt gaagtgcgta cggcgcttgt cacgacgtgt tttcgacgtt cttctttgag
     1921 gtactgccat gtttacacct ccttacccaa gttacgtgtt atttctactg tcagctttct
     1981 atttcttaaa gaaatcttgt aaccccgcca gtctcgggtc gattcgctct ttcgagcatc
     2041 cgcatggatg ttcgttgcgg ttcgtgccgc aagtcgggca caaccccttg cacgcctcgt
     2101 cgcaaagcgg cgcatacggc aagttcatca agagcgtctc ttccacataa ggcttcaagt
     2161 cgacaaggtc gtccgccacc aaaatgacgt cctcctcctc ttcttctgcg ggaagatcag
     2221 tagagtcctt cagcttgaac tgctcttcaa acggaataac gtacgtttcg gtaaacgatg
     2281 tcaaacaacg cgagcattgc agttccatct tagcggtcag cttgccatgt gctcgtgcga
     2341 gtccatcact ttccgccttt accgacagtt cgacatgaac cggttcagaa atggtaacat
     2401 cagagcgctc acgcgctacc tcatcggcac gaaatgccgt atcaatctgc gtttcccctc
     2461 taccagcaac tatgtctcga aatcgaaatt ccatactatc actccaaaca aacaaaatta
     2521 attatagcga ttaacgtcat gctttgtcaa ccaaattcgt ttgacagttt gccagagccg
     2581 gagcacgatt ccccttccta tggaaaacca ctctttcatg gtatgatttg attatcattg
     2641 cccgcggatt gtctgggaaa gagatccgtt aacaagcatt caatgtacct ataaaaagat
     2701 accattgaga tacaatatta ccgcattcgg cgcctgaaag gaagagatgc gaatgagaac
     2761 gctcggaatc gtcgttgaat acaatccgat gcataacggt catgtatatc atctccagca
     2821 atcgaaaaaa atttcaaatt gcgacgcagt tgttgctgtt atgagcggcc actttctaca
     2881 gcgcggcgag cccgcacttt ctgataaatg gtcgcggacg gatatggcat tggcgaacgg
     2941 tgtcgaccta gtgcttgaac tgcccgtcgc ctacgctgtg cagcctgccg aatggtttgc
     3001 ctacggtgct gtcgctacat tacaggccac aggagttatt gacgccctct gcttcggcag
     3061 cgagctggga gatgcagaac cgttcataca agctgcagat gtacttgtca acgaaccttc
     3121 cggtttcaca gatcaactgc aggagcattt aaaagctggc ttaacctacc ctgctgctta
     3181 tgcggcggcg gcgggacatg cgataaaaaa cagcgccact ctcccttcaa cgggccaggc
     3241 cgatggttgg atgattcagc cgaacaattc gctcggcttg cactatgtga tggcactgcg
     3301 gcgcttaaat agcacaatcg aggcctacac aatcgcacgc acgcaagcgg gttaccatga
     3361 tacaaacgct ccggaaggct caatcgcaag tgcaactgcc gtcagaaagc tgtgggtgga
     3421 aaataaaaat gggagccttg gcgcatgcgc acaatatttg ccgacaagta cgctccaaat
     3481 ccttgagcga gagcatcgtc ttggcagggc gccaatcagt tgggattcct acactcgcga
     3541 gttgatatac cgtcttcatc agctgtctgc taaatctcta gcgcgacacc ttgaggttac
     3601 agaaggcctc gagcatcgtg tcaagcaagc acttggcagg atggcggatt atcgggtaga
     3661 atcgctgctt gagcagctta agaccaagcg ctatacgcga acgaaactgc agcgtatgct
     3721 cacacatatt atacttcatc atgataagtt ccatttcgga cctgacagtc tggaaaaagg
     3781 accctggtac atacgagttc ttggttttac agataaaggg agagcactgc tcaaacgcat
     3841 gaagaaaagc gcagagctgc cgattgtatc aacagcaaac cgtaacaata tcggccttgg
     3901 cggcgtctgc ggcctggagg cagatattcg gtcaacatcg ctatatgcga atgcctttca
     3961 acaatggaac gcgcgggatg ccctccggga ttactatgag ccaccccgcc ggaactccta
     4021 gtatacagca agtcatggat cgaaaataaa gatcgagctt agaagtcttc cgtctaggct
     4081 cgatctttat tacgttgcac ctaacgatta cttcccagca ttctgcttac tactgaattg
     4141 cttgatcgct tgaagcgctt ctcccacggt tcgaactgga accagcttca tcttggtatc
     4201 catcgactca agcttcgctt tcgcttccgc ataattgcct tcggggacaa ggaatagctc
     4261 tgcctgctca cgatccgcag caaccacctt gaactgtact ccgccaatga tgcctacttt
     4321 accgtcagga tcaattgttc ccgtacctgc tattcgatgc ccttgcgtca agtctcctgg
     4381 tgtcagccga ttaataagct ccaatgtgaa catcagacct gcagatggtc cgccaatttc
     4441 actgtccttg aatgttacag ttttgccttt atcatcaggt gccgcttcct tgcgttgacc
     4501 gtatcgaagg ccgaagccaa cgcgcttctc ttctttcttg ttcggatcac taagctcaac
     4561 tagcgttgcc ttaacgacct tctgttgctt attccgctca actttaactt gaaccgtctc
     4621 ccctgccttg cgccccttca taacagcctg caaggaatcg aaatcgacaa cgggctgtcc
     4681 gtccacttcc acaattcgat catttgtctc aaattcatta tgcgcaaggt tcggataatt
     4741 ataaattaca aacacgcctt cagattttaa ttggtatgga atatgtaatt gattataggc
     4801 agcgagtaca gctccaagct gggagtcgct catattgaac aattgctccg taacatactc
     4861 ctgttcgctt ctaccttgca atgaatccgc ctttttcccg aactcggcat gcggatcgaa
     4921 tgatttccat ataagtaagg ccagattggc ataagtacga cgtaccgtgg tgagcataaa
     4981 agttccgtct tccgtctcat cacctgcttg cacgcttacc atcggcttga cctgctcagc
     5041 acttcccggt gtatatatta catagggcgt tggcatatat accatgatgt acaaaaacag
     5101 aagtgctgca aagaaccact tccagttgcc taatcgccaa cgcttcgtct tctgggtgcc
     5161 tagtaattcg tcttctttca ttgctgcatc cctcctgcta cattcactcg cctttgcatc
     5221 catgtacaaa ctggtctaaa gcctgtcttt gggacgtaca tatgtggaag tacgaacttc
     5281 cattttaatg caagaggtga acatctatgt tttcttttcg aacgccacta tcccagcatc
     5341 cgttcatgac attgctaatg ggaagcacgg cggttctttt cgttatttgc attgtatcct
     5401 atcccgatca agctttccaa gcctcgcttc aaggcctgaa aatatggtgg accattatat
     5461 tcccggcgtt gcttccgttc ttagtattgt ccgaaatgct gtatgcttac ggattcgtac
     5521 atggcctagg cgtcatgctg gatccaatta tgcgtttgct gttccgcctt cccggggttg
     5581 gcggctgggc atggtcagtt ggctggacag ccggctatcc agcaggtgca aaggctgttg
     5641 ctcaactgcg caagcagcag actttatcac gtcaggaagc cgagcgtctt ctctgtcttt
     5701 cccatgcgaa cagtcccata ttcatgattg ctgttgttgg agtagggttc atgcagcagc
     5761 cccatcttgg gcttgtcatc gcgatcgtcc actggctgtc cgctatcctt acgacactga
     5821 tttggcgaac gttacatcgt atgcatcctg agctgcaagc tgtacgtccc aaccataaca
     5881 tcactaaagc gcaccgcaat ttctatcgcc gattgattag cgaaatggag gacgcccatc
     5941 gacaagatgg ccgtcccttc gggagaatgc tcggcgagtc ggtcagctct gctgttcaaa
     6001 ccttaatgat ggtcggcgga tatatgatga ttttttccgt catcattcaa gttctgcgca
     6061 tcgcaatccc acaggagttc ggcaagcata tcctgaacgg attattcgaa gtaaacctgg
     6121 cagcctacac acttggttct acagcttttt cttcccccgt ttttcaggca gctttgctca
     6181 gtgctgttat tgcctggagc gggattagtg cccttcttca ggttcatagc atcattaagg
     6241 aaaccgatct gcgtttcagc cattttctga tttcgcgcct gcttcatgca ttatgtgcat
     6301 ttctgcttac ctatgtgctg tggtcgccat tacacgcttg gctttccact ccgaattcgg
     6361 caatgtcaac attaagtccg tttgcagcca gccataccga tgtcgaacat gtatcttcca
     6421 ttcttgactc cagcgggtca ctgcccttta taacctatat gagtcagctg ccgatttggg
     6481 cccaagcatc tatactcatt cccgtagcct gtctatttct gttcgcatta ttcgttctgg
     6541 cacaaacgat aggcagactt tttacccatc gctaaacaaa aaagtccata tcgaccggat
     6601 catcctactg gcgacatgga cctgctgctt agttcgtatc tttgaattta tcacgcagtg
     6661 ccaattcaac ttctggtgca actaattcac taacgtcacc attaaatttc gcaatttcct
     6721 tcaccatact ggagcttaaa taggaatatt tcggattcgt catcataaag atggtctcta
     6781 cccttggatt gagcttttgg ttcgttgacg ccatttgcag ctcatattca aagtctgtta
     6841 cagagcgaat gccgcgtacg atcacatggc cctgcttctg ctccatataa ttgaccagca
     6901 aatcgcgaaa gctgtcgatt tccacattcg ggatatgtct agtaatttgc cgcagaagct
     6961 cctttcgctc ttctacactg aacgtaggct gcttggacaa attgttcagc actgcaacga
     7021 taagcttgtc gaactgtttg gcagcccgct gtatgatatc aagatgtcca agcgtaaccg
     7081 gatcgaagct tcccggatac accgcaatgc gcggctcatg cactcgttct gtatgttccg
     7141 tcatgaaata aatcctcctt attccctgca gcagatagat ctgccgtttc atttcctcat
     7201 atggtttgaa gcttatgcgt cttgttcttg cgccgcttcc ttctctgtct gtccgtagac
     7261 tcggtatacg gttacaccga tctctccata ctccgccttc ttccacacat aaagggggcc
     7321 aatctcattc gggtaaacat gtttcgaatc atgttctaca acaatgattg cgctctctga
     7381 gattaaccca tcattccaca tgccttccag cagttcgtcc gcatccttca gccgatatgg
     7441 cggatcaaga aaaataagct caaacgcaat gtcacgcttc gccagcgcct ttaacgcccg
     7501 tttcgcatca ttgcgatata cttcacttcg attcgcaacc ccagccgttt gaacattatg
     7561 cttgacgact tcaacagcct tgggatcctt gtctacgaat attccttgtc ccgcgccacg
     7621 gcttaacgcc tcaatcccta ggccgcctgt gccagcgaat aaatccagca catagccgcc
     7681 gtcaaaatac ggcccgatca tgctgaacaa tgcttccttg actttatctg tggtcggtcg
     7741 cgtccccatg ccgggaaccg gctttagtga gcggcctctt gctgttcctg atataactct
     7801 caatgcactc cacccattct tttgcgccgc agcgcagctt atcacgtatg atcatgatgg
     7861 tatgtaacaa ataacttatt gtatttcgac gtttatcgta ccacatttct atataatttg
     7921 aaaaaggaaa acgtgtccta acatcagtca tccggtctca cttttctaag aaaaagcatc
     7981 gaatgtaaaa actccttacc aggggggccg cgtccgataa ggagtgtctt cttcctaatt
     8041 atgtctttct gtttcttttc tttctaaatt ctttcactca aatgagcaga actctccgtc
     8101 cttatttgca gcttccctta tttgtgcagc ttttgcagca gctccctcac gcttattagc
     8161 atttgcctgc gagcttcttc atcaatagtc gttactgagg caaaaaaacg atgagcgagc
     8221 accttcattc ccacaaattc aaatattcca ttatccgtcg tttgctgcat cgactcatac
     8281 atcccgctct gctcatactg ctcatgcgaa tttccatgcg tggacagcag cagtgcagac
     8341 tttcctttca acagcttctc gatgccgtcc gcgccatatc gataagcgaa tccataagaa
     8401 aatacacggt caatgtaccc cttcataatg gcaggcatgc ttgcccacca gattgggtaa
     8461 accatgatca tatgatccgc ccacgcaaca tgtccctgct cctcctcaat atctgaaggc
     8521 agcttatgct gcccgaacag ttcgaaatcc ttggcagaca gcactggatt gaactccatt
     8581 tcatacaaat tgcgaactaa tgccgcatgc ccttgatctt caagctcagc ttggacgatt
     8641 tccataatcg catgattgaa gctgttcgga ttgggatgcg cgtatataat cagatgcctc
     8701 atttagatct cctcctactt gttcaccaaa gttgcgctaa cacaactgga caaatctgtt
     8761 ttaatatagg atgtgagaaa gcggcatcat ttatcgagct ccacaaatat ttttatggca
     8821 tgtatatttc tgaccaatcg ggcaatcatt aaagtatcga catcatcgaa tgtcgtagac
     8881 aaccgcggag aagacttacg tttccccata agctcttcgt ccggtttctc ctctcccatg
     8941 agggcgtcct agcaataggg cgcccgtttt aatttgcctt aataccttat atataaagaa
     9001 ttaatccatt attctcagaa tggtgatttc ctctaaaagc aatgattggg gactaaatgg
     9061 ggactaactt tcaaatgcag ttatgttttt cgatgtcttc gaatgcttca ttcatcattg
     9121 cttcaatttc aagacatgat ggaaaaagtt ctggatttac atgtttaatt tcgtggacaa
     9181 aaatattgaa caacgcataa aacttttctc tttctctttc ttgttgtttg acttcatctc
     9241 tgtattgaat atttgaggtt cgttcattgt ttttagtttc ggagcgtttc gacatttctg
     9301 tgtcctccct catcttctgt atcatcttat tttgttacag ggtgtacacc ttgtgaaaaa
     9361 aatgatagca catggttatt tttctcactg tcgaatctag tcgaacctca ttcataaatt
     9421 gatactagta tcaatttatc tttacattac ttcaacaccc ggtgtatgat gtttatttac
     9481 acaaaaaaaa gaagaccctc aggtcttctt aattaagtgc tctatatatt ttctctttac
     9541 ccttgaaacg ttagcggttt tagtacctgt tggaaagtaa gctgtgatcg caaatggaga
     9601 ttcagtcaca tatgtaattt gatcaatatc cactaagttg ccgctatcaa gttttgttaa
     9661 atgtttaaaa gtcactttac atgattcaag tgtcaaaaaa accgtaaact cccctttttt
     9721 tgtatgaaat cggggaacaa aataatttga tttcggttgg aacacatcaa tgaagaatat
     9781 ttcttttggt ttaaagaagc catattcagt agtaactttg tcatcaatga cgcgcatccc
     9841 caaaatttct acattatcct ccatacctgc gttacctact tcttgctgtc ttttagttcg
     9901 gcaggcaatt cttttgcacc gataaatgtc ttcttattac gcgcggacaa cttagcactc
     9961 gcgagtaaaa agtcggctat acgtcgagct gtctgttgtt tcatagtttg cgtccccctt
    10021 tccaaggtag tataagtatt ccctggacca tgaacactaa tgcaagtaca ggggatacga
    10081 taatgaaatt aaacaagaca attattacgg ctagtatctt cgaaaccgta tttgacaata
    10141 atggccttgc taattcttcg tacgtattcg gggcacatat cagaaaaata ccgactgcca
    10201 cagtgtttaa tattagcact attgacgcag atagttcaat gtgcggtatc aatgtaaaaa
    10261 ttaatgctga tacaatagca caggccgtta gtgatttcat gtgtactccg ccagaaaaat
    10321 acctaagaat tccgaatgct gcagcagcga tcagcgtatt aattatttct cctgttaccc
    10381 atccacccaa acaactaagt attacagtaa cgaaaaaatt cagatataca cctaatccat
    10441 attccattac ttctacactc gtttgacctt ctggatctgc tgccttgatc ctgagagcca
    10501 tataattcgc ccatctttct atcattaatc ttcaatatcc cttctgcgtg actgcatata
    10561 gagcgcaccg aatgtgatca ataacagcgg aataacatat tcaatcttca tgtgaaatag
    10621 tgcagttaat gagatcgaca gcacaaccat agtggctaac gaccagaaca atagtgcttt
    10681 atttctggac gtcgaatagt cttccttcac actaaaatca tgtggagggc gaattataaa
    10741 agaaaatccg agattaaatt tctttaagat atatcctatc aagtaggctg atacgattgc
    10801 cgtaatctga aaaatattga cggtcatccc tgtactttgc aatgcaaatt gttgatttac
    10861 accaatagaa gtaaatgcta tgtaaataag aagttggatt aacagataac cgcctagacc
    10921 taatgctgta attattccac tataaaaaag ttttaccatc atcacgtacc gaataaataa
    10981 cgtaaaaagt aatagttgca gcggtaaatc catgaccggc acgtctaaaa caattctcat
    11041 aaaatatgaa actatagcaa taaagacgga catgatgcct agttctttta tatattccct
    11101 tagtggaagc ctgtagagct ttaaaattaa tacgaaagct gcaaacgcgt ccatccctcc
    11161 taggaatata taaattgatg ttgatgttga gtccaaatta ctacacctcg ttcattaaaa
    11221 tgtgacattc atactatccc attatactat taggttgaat gaaaaaaagc ctcggactaa
    11281 aatccggggc tttacttctt cgatgacgat cgcattgtgt catagactgg tttttggcag
    11341 cgtatacaca tgtacactcc atccacccat ttacccttca tagcagcttt gcagttgcac
    11401 aaatagggct gcactggttc aattgtcttt tgagttggca gagtccattg catttcttcc
    11461 cgccaatcct tcataacatc accccacgca gaacatttgt tcttattata aattaataat
    11521 catcttttgt tcaacacagg aataatttta gaataattta catcgatttt gacaagatag
    11581 cgcggttgca aaaaacaatg cattattgta gaacggttgc aaaagcatag ccaactacat
    11641 tgacgaacaa cgggagcgtt tgtaaatatc aaaacaataa agagccaagg aactatt
//
