LOCUS       NZ_CP058354.1          10918 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP058354.1 Bradyrhizobium japonicum strain 5038 chromosome,
            complete genome.
ACCESSION   NZ_CP058354
VERSION     NZ_CP058354.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:57:06
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 2247219
            Orig. end    :: 2258137
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10918
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[2252219:2253137](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5918)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..10918
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10918
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193)"
                     /tool="antismash"
     CDS             730..1431
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 78.7; E-value: 1.2e-23)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_2012"
                     /transl_table=11
                     /translation="MAVHTSSAAEAAAPTTRERILSEALNLFAHSGYGGASMRELARRV
                     GIRESSLYNHFSGKAAILEAIVAEHGPASSASRLEEPRYKQLARQPAAFCRQFALDLVE
                     QWSDPREHQFQKVITAERNRVPGIRAKFADHFYAREQSMMTDYFRGFALAGLVSTPDPR
                     ETARLFAAGLIYIRLEHYVMGAAPSPRPKVIEAINRYLAFFLSLIAADGGTSDNKKRKP
                     KGETRGKAAPD"
     PFAM_domain     787..927
                     /aSDomain="TetR_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00440.25"
                     /description="Bacterial regulatory proteins, tetR family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2012_0099"
                     /evalue="2.40E-14"
                     /label="ctg1_2012"
                     /locus_tag="ctg1_2012"
                     /protein_end="66"
                     /protein_start="19"
                     /score="52.9"
                     /tool="antismash"
                     /translation="ILSEALNLFAHSGYGGASMRELARRVGIRESSLYNHFSGKAAILE
                     AI"
     PFAM_domain     1015..1341
                     /aSDomain="TetR_C_7"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF14246.8"
                     /description="AefR-like transcriptional repressor,
                     C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2012_0100"
                     /evalue="1.00E-07"
                     /label="ctg1_2012"
                     /locus_tag="ctg1_2012"
                     /protein_end="204"
                     /protein_start="95"
                     /score="32.1"
                     /tool="antismash"
                     /translation="RQFALDLVEQWSDPREHQFQKVITAERNRVPGIRAKFADHFYARE
                     QSMMTDYFRGFALAGLVSTPDPRETARLFAAGLIYIRLEHYVMGAAPSPRPKVIEAINR
                     YLAFF"
     CDS             1409..1960
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298:putative carboxymuconolactone decarboxylase
                     (Score: 134; E-value: 6.9e-41)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2013"
                     /transl_table=11
                     /translation="VARLPLIDPETTSGDIRASFDRMPVKLNIFRMMAHAEANMIPAMR
                     LGNSILHKQKLSAVNRELLILQAAQLEGGAYEWRQHVPIALGVGCTQGQVDAVERTEYD
                     SAALSEAERVLLKFGREIVENVRVPEATFAAARKHFSDQEIVESIVALGFYMMMARLTE
                     ATETDLDPAAGMKVYDGGKK"
     PFAM_domain     1538..1738
                     /aSDomain="CMD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02627.22"
                     /description="Carboxymuconolactone decarboxylase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2013_0101"
                     /evalue="6.90E-10"
                     /label="ctg1_2013"
                     /locus_tag="ctg1_2013"
                     /protein_end="110"
                     /protein_start="43"
                     /score="39.0"
                     /tool="antismash"
                     /translation="MRLGNSILHKQKLSAVNRELLILQAAQLEGGAYEWRQHVPIALGV
                     GCTQGQVDAVERTEYDSAALSE"
     CDS             1970..2818
                     /NRPS_PKS="Domain: PKS_KR (34-208). E-value: 1.2e-06.
                     Score: 20.3. Matches aSDomain:
                     nrpspksdomains_ctg1_2014_PKS_KR.1"
                     /NRPS_PKS="type: other"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     167.8; E-value: 4.9e-51)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2014"
                     /transl_table=11
                     /translation="MSENSETKPDRYVRPPSPESLGEAPGRGRLKGRRILIVGGGQRVF
                     DAATDPIGNGRAMSILCAREGAKVAVADLNHTSARQTVKHITDEGGEGFAITADVASEA
                     DVQRMIEEAHRAMGGLDGMVLNIGTFGKTGLDAVSPEEWNKIYDVNVRGPMLCCRAAMP
                     KFDNGGAIVFISSIAALKAGSQMAVYDSSKAALGGLMRNIAHLGSRRGIRANLVYPGLV
                     DTPNGREAGAGRPSRGKGHVPFGRQATAWEIAYAVLFFLSDESVYVTAQTLAVDSGLSG
                     M"
     aSDomain        2072..2593
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_2014_PKS_KR.1"
                     /evalue="1.20E-06"
                     /label="ctg1_2014_PKS_KR.1"
                     /locus_tag="ctg1_2014"
                     /protein_end="208"
                     /protein_start="34"
                     /score="20.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ILIVGGGQRVFDAATDPIGNGRAMSILCAREGAKVAVADLNHTSA
                     RQTVKHITDEGGEGFAITADVASEADVQRMIEEAHRAMGGLDGMVLNIGTFGKTGLDAV
                     SPEEWNKIYDVNVRGPMLCCRAAMPKFDNGGAIVFISSIAALKAGSQMAVYDSSKAALG
                     GLMRNIAHLGS"
     PFAM_domain     2132..2809
                     /aSDomain="adh_short_C2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13561.8"
                     /description="Enoyl-(Acyl carrier protein) reductase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2014_0102"
                     /evalue="6.20E-58"
                     /label="ctg1_2014"
                     /locus_tag="ctg1_2014"
                     /protein_end="280"
                     /protein_start="54"
                     /score="196.2"
                     /tool="antismash"
                     /translation="GRAMSILCAREGAKVAVADLNHTSARQTVKHITDEGGEGFAITAD
                     VASEADVQRMIEEAHRAMGGLDGMVLNIGTFGKTGLDAVSPEEWNKIYDVNVRGPMLCC
                     RAAMPKFDNGGAIVFISSIAALKAGSQMAVYDSSKAALGGLMRNIAHLGSRRGIRANLV
                     YPGLVDTPNGREAGAGRPSRGKGHVPFGRQATAWEIAYAVLFFLSDESVYVTAQTLAVD
                     SGLS"
     CDS             complement(2883..3182)
                     /locus_tag="ctg1_2015"
                     /transl_table=11
                     /translation="MALHFMRVETPVHELEIWMACSGGFSFVISSESRTGPGLHGKPGL
                     VASWRPVEENKSAIKLGGSPFSTFAEAEKACEALLEALEQMSASQTPTVAAPPQ"
     CDS             complement(3238..3435)
                     /locus_tag="ctg1_2016"
                     /transl_table=11
                     /translation="MPVLDLPYWLIIAGSALVLGGLIGLGVSRNKRVESDPIMLPGDLT
                     TWKAEGSETSDLAAARDSHR"
     CDS             complement(3501..3722)
                     /locus_tag="ctg1_2017"
                     /transl_table=11
                     /translation="MHVPEAGAALRGGQHTEFQPALCNREHAMLACLTRTERNKFEPLL
                     AGGNPGQKSTPFGYLARSLERRIIQTLS"
     CDS             complement(3723..4232)
                     /locus_tag="ctg1_2018"
                     /transl_table=11
                     /translation="MITDQRMPASTNLPPLGLLVDKANHLVQTIAAPSIVQLVLRVALA
                     VPFWRSGILKWGGFLRLNDTAVTLFSDEFMLHLPGGPYHFPAPTVMAFLSGCGEITFPI
                     LLILGLGTRFAGLGLLFMTLIVELTVPDGWPIHLTWAAMALAIMAYGPGRVSLDHLLGR
                     ALSPER"
     PFAM_domain     complement(3843..4124)
                     /aSDomain="DoxX"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07681.14"
                     /description="DoxX"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2018_0103"
                     /evalue="1.30E-17"
                     /label="ctg1_2018"
                     /locus_tag="ctg1_2018"
                     /protein_end="130"
                     /protein_start="36"
                     /score="64.3"
                     /tool="antismash"
                     /translation="QLVLRVALAVPFWRSGILKWGGFLRLNDTAVTLFSDEFMLHLPGG
                     PYHFPAPTVMAFLSGCGEITFPILLILGLGTRFAGLGLLFMTLIVELTV"
     CDS             complement(4229..5011)
                     /locus_tag="ctg1_2019"
                     /transl_table=11
                     /translation="VRPEPDLRFAAAFAPALLDPSREVPSTVAGRDGKPAGRRYDVYRN
                     NVTVSLIDALAAIYPAVQRITGTDFFRAIARFYIRSGPPTSPLLFDYGRGFPDFIAAYE
                     HARDMPWLADVARIERAWLDAYHAMDASPLSPSRLSAIAPDQLGDLVFTPHPAAQIAQS
                     QYSAVTIFAANRDGAPASRIDASAPEDALITRPAYDVVVRHLPPGGAVFANCLLCGQPL
                     GAAAAMAFDASAEFDIAANIAGLIEAGAFTSFASGDAS"
     PFAM_domain     complement(4712..4984)
                     /aSDomain="DUF2063"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF09836.11"
                     /description="Putative DNA-binding domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2019_0104"
                     /evalue="2.70E-23"
                     /label="ctg1_2019"
                     /locus_tag="ctg1_2019"
                     /protein_end="100"
                     /protein_start="9"
                     /score="82.1"
                     /tool="antismash"
                     /translation="AAAFAPALLDPSREVPSTVAGRDGKPAGRRYDVYRNNVTVSLIDA
                     LAAIYPAVQRITGTDFFRAIARFYIRSGPPTSPLLFDYGRGFPDFI"
     CDS             complement(5001..5918)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_2020"
                     /sec_met_domain="DUF692 (E-value: 1e-84, bitscore: 282.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAINQDRPATVPIRFPAPIGGVAGTSFKPEHLPAILAAEPRRG
                     FFEVHAENYMGAGGPPHRALEAIRRDHPLSLHGVCMSIGGPQPLDKAHLARFRNLVARY
                     QPALVSEHLAWSSHETSFFNDLLPLPYTEATLACVCDHIDEVQEAIRRPLLLENPSTYV
                     AFRESSMSETDFIRAIVARTGCGLLLDINNVLVSATNHGFSALDYLTDFPLADVAEIHL
                     AGHAEQTDDDGHLLLIDSHDGPVADAVWRLFEIIVAQRGPMPTLIEWDSKIPQWPILQA
                     EAAAAQAILDRRQPIAKAENRRAA"
     PFAM_domain     complement(5049..5846)
                     /aSDomain="DUF692"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF05114.15"
                     /description="Protein of unknown function (DUF692)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2020_0105"
                     /evalue="5.90E-103"
                     /label="ctg1_2020"
                     /locus_tag="ctg1_2020"
                     /protein_end="290"
                     /protein_start="24"
                     /score="343.8"
                     /tool="antismash"
                     /translation="AGTSFKPEHLPAILAAEPRRGFFEVHAENYMGAGGPPHRALEAIR
                     RDHPLSLHGVCMSIGGPQPLDKAHLARFRNLVARYQPALVSEHLAWSSHETSFFNDLLP
                     LPYTEATLACVCDHIDEVQEAIRRPLLLENPSTYVAFRESSMSETDFIRAIVARTGCGL
                     LLDINNVLVSATNHGFSALDYLTDFPLADVAEIHLAGHAEQTDDDGHLLLIDSHDGPVA
                     DAVWRLFEIIVAQRGPMPTLIEWDSKIPQWPILQAEAAAAQAIL"
     CDS             complement(5958..6263)
                     /locus_tag="ctg1_2021"
                     /transl_table=11
                     /translation="MSAKHAITTLALAGAMSTALATLASAAPLTKAEADAAVAAKKEKC
                     FGVALKGQNDCAAGPGTTCQGTSTTDFQGNAWKFVQGGTCTSIDLPGGKKGSLKPV"
     PFAM_domain     complement(5985..6140)
                     /aSDomain="DUF2282"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF10048.11"
                     /description="Predicted integral membrane protein
                     (DUF2282)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2021_0106"
                     /evalue="8.40E-23"
                     /label="ctg1_2021"
                     /locus_tag="ctg1_2021"
                     /protein_end="93"
                     /protein_start="41"
                     /score="80.3"
                     /tool="antismash"
                     /translation="KEKCFGVALKGQNDCAAGPGTTCQGTSTTDFQGNAWKFVQGGTCT
                     SIDLPGG"
     CDS             6576..8663
                     /locus_tag="ctg1_2022"
                     /transl_table=11
                     /translation="MNVDPGRHLHAIARLTVSIRLAVSALVLAAILLTAALSSLLWWRT
                     AEATSWQLASTINEQIVAAVRKEVAAIVDEARAAHTAIRTLFLQNVLDTREADKREFVF
                     LSQLQSQATISWVAFGWPDGSFFAAHKLGDRRLEMMEISLADHPGHRRVDEYDVVPGDI
                     EFANRRFEPTDFRVADQAWFKAGLSADDPQWFRVMDHPTGARPSIAFVGPIDVYQERQG
                     VLAVVIEYTRLARFLSQLEVGRTGTAFIVDDGGELIAAPDKEADELHPARGDTTLLPLA
                     RVALEKAGEAGRKEAWRSRLNSDGGAYEVALTPLPFPGWSLATVIPEAEFLGPVETTLR
                     RLILGLAVGAVLAALASAMLARSVIAAPLSRVVGELRHVEAFALEQVRRHPSRLKEIAS
                     LSGAIAEMASGLSAFRKFIPADLVRALLRQGVEARPGGSIQELSVMFIDIAGFTGLSER
                     LGDRVVPLLSRYLDVTSEIIVANGGTIDKFIGDAVMAFWGAPQPQEDHAARCCRAALAC
                     CRAIEASGLADDLGQPLQIRIGINSGRMLVGNIGSELRLNYTVIGDAVNVASRLEGANK
                     SYGTQILIGESTERLARGAVVTREVDSIAVYGREEGLAVHELIGIVDEDAGMAGWIARY
                     EQGLANYRARRFAVALADFEAVLAERGHDRPSELMRERCRQFAATMPDAAWQPVVALRT
                     K"
     PFAM_domain     7068..7547
                     /aSDomain="dCache_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02743.20"
                     /description="Cache domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2022_0107"
                     /evalue="1.50E-14"
                     /label="ctg1_2022"
                     /locus_tag="ctg1_2022"
                     /protein_end="324"
                     /protein_start="164"
                     /score="54.2"
                     /tool="antismash"
                     /translation="FANRRFEPTDFRVADQAWFKAGLSADDPQWFRVMDHPTGARPSIA
                     FVGPIDVYQERQGVLAVVIEYTRLARFLSQLEVGRTGTAFIVDDGGELIAAPDKEADEL
                     HPARGDTTLLPLARVALEKAGEAGRKEAWRSRLNSDGGAYEVALTPLPFPGWSLAT"
     PFAM_domain     7887..8408
                     /aSDomain="Guanylate_cyc"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00211.22"
                     /description="Adenylate and Guanylate cyclase catalytic
                     domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2022_0108"
                     /evalue="1.70E-29"
                     /label="ctg1_2022"
                     /locus_tag="ctg1_2022"
                     /protein_end="611"
                     /protein_start="437"
                     /score="102.9"
                     /tool="antismash"
                     /translation="SIQELSVMFIDIAGFTGLSERLGDRVVPLLSRYLDVTSEIIVANG
                     GTIDKFIGDAVMAFWGAPQPQEDHAARCCRAALACCRAIEASGLADDLGQPLQIRIGIN
                     SGRMLVGNIGSELRLNYTVIGDAVNVASRLEGANKSYGTQILIGESTERLARGAVVTRE
                     VDSIAVYGREE"
     CDS             8812..9333
                     /locus_tag="ctg1_2023"
                     /transl_table=11
                     /translation="VTNVSYTVPPNWTAGSDKTGASKNKNPNDPNMLTTGAYGSAFELL
                     LDNRTNRDGTTTETLTYKSYSGKTNGTFSATAETGTGADLGSAYQALLATLQANGEMDD
                     KTAAQLDGAMGKLDTQSESGAKDAEVSGGGLLVQAGGPVYFSGISAYQNQFVDSLVNEL
                     STRLNLDAKA"
     CDS             9435..10019
                     /locus_tag="ctg1_2024"
                     /transl_table=11
                     /translation="MKTTLLKSEDYTRSPWKNGGGIFTDIADAHRADAQAKDWDSLLWR
                     FASTPIVAPGPFSYMPGIDRLQMVVGGRGLVLKSPSQDFDEREPFTTVRFTGEMEIVTE
                     LEAGPVEVVNLMARRGAAEIELLALREPGERPLSAGTHLVYAVSGDCSIRLNSEDIVIP
                     AGSTLKVELSVASTLALVSGLAVLASIQLVG"
     PFAM_domain     9459..9956
                     /aSDomain="HutD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF05962.13"
                     /description="HutD"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2024_0109"
                     /evalue="2.90E-30"
                     /label="ctg1_2024"
                     /locus_tag="ctg1_2024"
                     /protein_end="174"
                     /protein_start="8"
                     /score="105.7"
                     /tool="antismash"
                     /translation="EDYTRSPWKNGGGIFTDIADAHRADAQAKDWDSLLWRFASTPIVA
                     PGPFSYMPGIDRLQMVVGGRGLVLKSPSQDFDEREPFTTVRFTGEMEIVTELEAGPVEV
                     VNLMARRGAAEIELLALREPGERPLSAGTHLVYAVSGDCSIRLNSEDIVIPAGSTLKVE
                     LSV"
ORIGIN
        1 agatcgtcgt tctggtagag cacgccgatc ttcgcattgg cgtggttcgc cttgatgtat
       61 ttggcgtagg cgatcgcctc gtcgcgatag gtcggctgcc agccgacggt ccacggaaag
      121 ttcttggaat cgtcccactt cgcggcgccc gagcccagga agagttgcgg caccttgcgg
      181 tcgttgagat atttgtgcac ggcgctgttg gtcggcgtgc cgacgccgcc gaagatcagc
      241 agcacctcct cgctttcgac cagtcgccgg gtctgctcca ccgtcttcgg cgggctgtag
      301 gcgtcgtccg cgatgatcat ctggatgcgt cggccgttga cgccgccctc gtcgttcacc
      361 ttgcggaaat aggcctccgc cgatttcgcg atctgcgcga aggccgagac cgggccgctc
      421 agcggcgcgg tggtgccgat cttgatggtc ttgtcgtcgg cgccgggatc gtactttgca
      481 ttctgggcgt gaacggcgct ggctgcgagc agcggcaaca ggatgcaggc cgcacgaagg
      541 cgaaggcggg caaagcgcgc catgaggtcg tctccccaaa ttgctctccg cctctcattg
      601 gagcggagtt gaaacgcgag aacagcgccc gccgtaagct tgcgggccgc gaccgttgcc
      661 gccagactgg cgaaacgaac gatcgttcgt acggttgact agcgaacgat cgttcgttag
      721 tcaacccgta tggctgtcca cacctccagc gcggcggaag cggctgcgcc cacgacccgc
      781 gagcgcatcc tctccgaagc gctcaatctg ttcgctcata gcggctatgg cggcgcctcg
      841 atgcgcgaac tcgcacggcg cgtcggcatc cgcgagagca gcctttacaa tcacttctcc
      901 gggaaggccg cgatcctcga agcgatcgtc gccgagcacg gcccggccag ttcggcgagc
      961 cggttggaag agccgcgcta caagcaactc gcgcgccagc ccgcagcgtt ttgccgccag
     1021 ttcgcgctcg atctcgtcga gcaatggtcc gacccgcgcg agcaccagtt ccagaaagtc
     1081 atcaccgccg agcgtaaccg cgtgcccggc atccgcgcca aattcgccga ccatttttac
     1141 gcgcgcgagc agagcatgat gacggattac ttccgtggct ttgcactcgc cggcctggtc
     1201 tcgacgcccg atccgcgcga gaccgcgcgc ctgttcgctg ccggcctcat ctacatccgc
     1261 ctcgaacatt acgtgatggg cgccgcaccc tcgccgcggc cgaaggtgat cgaggcgatc
     1321 aaccgctatc tcgccttctt cctgtcgctg atcgcggcgg acggcgggac aagcgacaac
     1381 aagaaacgaa aacccaaggg agagacgcgt ggcaaggctg cccctgattg atccggagac
     1441 gacgagcggc gacatccgcg cctcgttcga ccgcatgccg gtcaagctca acatctttcg
     1501 catgatggcc catgccgagg ccaacatgat cccggcgatg cggctcggca attcgatcct
     1561 gcacaagcag aagctcagcg ccgtcaaccg cgagcttttg atcctgcagg cggcccagct
     1621 cgaaggcggc gcctatgaat ggcgtcagca cgtgccgatc gcgctcgggg tcggctgcac
     1681 gcaaggccag gtcgatgcgg tggagcgcac cgaatacgac tctgctgcgc tgagtgaggc
     1741 cgagcgcgtg cttctcaaat tcggccgcga aatcgtcgag aacgtccgcg ttcccgaggc
     1801 gaccttcgct gccgcgcgaa agcatttcag cgaccaggag atcgtcgaat ccatcgtcgc
     1861 actcggtttc tacatgatga tggcgcgcct caccgaggcg actgaaaccg atctcgatcc
     1921 cgcggccggc atgaaggtct atgacggcgg caagaagtag gcgggtggca tgtctgagaa
     1981 ttccgagacc aagccggacc gctacgttcg tccccccagt ccggaatcgc tgggcgaggc
     2041 gccgggcagg ggacgcctga aagggcgccg catcctgatc gtcggcggcg gccagcgcgt
     2101 gttcgacgcc gccaccgacc cgatcggcaa cggccgcgcg atgagcatct tgtgcgcacg
     2161 cgagggtgcg aaggtcgcgg tggccgacct caaccacacc tctgcacggc agaccgtcaa
     2221 gcacatcacc gacgagggcg gtgagggctt tgccatcact gccgacgtgg cgtcggaggc
     2281 tgacgttcag cgcatgatcg aggaggcgca tcgcgcgatg ggcggcctcg acggcatggt
     2341 gctgaacatc ggcaccttcg gcaagactgg gctcgatgcg gtcagccccg aggagtggaa
     2401 caagatctac gacgtcaacg ttcgcggtcc catgctctgt tgccgcgcgg cgatgccgaa
     2461 attcgacaat ggcggcgcca tcgtcttcat ttcctcgatc gccgcgctga aagccggctc
     2521 gcagatggcg gtgtatgact cttcaaaagc cgcgctcggc ggcctgatgc gcaacatcgc
     2581 ccatctcgga tcgcgccgcg gcatccgcgc caatctcgtc tatcccggcc tcgtcgacac
     2641 ccccaacggc cgcgaagccg gcgccggccg cccctcgcgc ggcaagggcc acgtcccctt
     2701 cggccgccag gccacggcct gggagatcgc ctatgccgtg ctgttcttcc tgtcggatga
     2761 aagcgtctac gtcaccgcac agacgctcgc ggtcgatagc ggcttgagcg ggatgtgaga
     2821 cgccagcgca atccggcgtc gcggaattcg atgccccgac gaagccgagg tccctcttca
     2881 tttcattgcg gcggggccgc aaccgttggc gtttgcgacg cgctcatctg ttcgagcgct
     2941 tccaggagcg cttcgcaggc cttctcggct tcggcaaagg tactgaaggg ggagccgcca
     3001 agcttgattg cggacttgtt ttcttcgacg ggtcgccatg aggcaaccag gcccggcttt
     3061 ccgtgaaggc caggaccggt gcggctttcg ctgctgatga caaacgaaaa tccgccgctg
     3121 cacgccatcc agatttcaag ttcatgcacc ggggtctcga cgcgcataaa gtgcagggcc
     3181 atgtctctct ccagagtttg ctccgcagaa tcgaccacgg gcgcaagccg tcagctccta
     3241 cctgtgcgaa tcccgggcag ccgcaaggtc cgaggtctct gatccctcgg ctttccatgt
     3301 cgtcaaatcg cccgggagca taatgggatc gctctcaacc cgcttgttgc ggctgacccc
     3361 taagccaatg agtccgccca ataccaacgc ggagcctgca atgatcaacc aatacggcaa
     3421 gtccaacacg ggcattgcaa ttctcccact gagaggagca cgttacgcaa ttgcatggcc
     3481 gaagcgcgaa ccgattacct ttagctaagt gtctggatta tgcgacgctc caagcttcgt
     3541 gcaaggtacc caaaaggggt agatttctga cctggattgc cgccggcgag caggggttcg
     3601 aatttgttgc gctcggtgcg cgtgaggcag gccagcatgg cgtgctcccg gttgcacaaa
     3661 gcgggctgga actcggtgtg ctgaccaccg cggagagcgg ctcccgcttc agggacgtgc
     3721 atctatcgct cgggcgatag cgcgcggccg aggagatgat cgagtgacac gcgcccgggt
     3781 ccgtaagcca tgatggcgag cgccatcgca gcccaggtga ggtgaatcgg ccagccatcg
     3841 ggcaccgtga gctcgacgat cagggtcatg aacaggagcc caagcccggc gaaccgcgtg
     3901 ccgagtccca ggatcagcag gatcggaaag gtgatctcgc cgcacccgga caggaaggcc
     3961 atcaccgtcg gtgccgggaa gtgatacggc ccacccggca aatgcagcat gaactcgtcg
     4021 ctgaacagcg tcacggctgt gtcgttcagc ctgaggaagc cgccccattt gaggataccc
     4081 gagcgccaga acggaactgc aagtgccacg cgcagcacga gttgaacaat cgacggggct
     4141 gcaatggtct gcaccaggtg attggccttg tcgacgagca gcccgagggg tggaaggttg
     4201 gtgcttgcgg gcatgcgttg gtccgtgatc atgatgcgtc tccggaagcg aacgaggtga
     4261 atgcgccggc ttcgatgagg ccggcgatgt tcgcggcaat gtcgaattca gctgacgcgt
     4321 cgaacgccat cgccgcggct gcgccgagtg gctggccgca caagaggcaa ttggcaaaga
     4381 cggcgccgcc gggtggaagg tggcgcacca cgacgtcgta tgcgggcctc gtgatcagtg
     4441 cgtcctccgg cgcggaggcg tcgatgcgtg aagccggagc gccgtcgcgg ttggcggcga
     4501 agatcgtcac ggccgaatat tgcgattgtg cgatctgcgc cgccgggtgc ggcgtgaaaa
     4561 cgagatcgcc gagctggtct ggcgcgatcg cggacaagcg tgacggcgac agcggtgatg
     4621 catccatggc gtgataggca tcgagccagg cgcgctcgat gcgcgcgacg tcggcgagcc
     4681 acggcatgtc ccgcgcatgc tcgtaggctg cgatgaaatc cgggaaaccg cgcccgtagt
     4741 cgaacagtag cggcgaggtc ggcggcccgc tgcgaatata gaagcgtgcg atcgcgcgga
     4801 aaaagtcggt gccggtgatg cgctgcaccg ccggatagat cgcggccagt gcgtcgatca
     4861 ggctgaccgt gacgttgttg cggtagacgt catagcgccg tcctgccggc ttgccgtccc
     4921 gccctgccac ggtcgagggc acctcgcgcg agggatcgag cagcgccgga gcgaaggctg
     4981 cggcaaagcg aaggtctggt tcaggccgca cgacgattct ccgccttcgc aatgggctgg
     5041 cggcgatcga ggatcgcttg cgcggcggcc gcctcggctt gcaggatcgg ccattgcggg
     5101 atcttgctgt cccattcgat cagtgttggc atggggccgc gctgcgcgac gatgatctcg
     5161 aacagcctcc acacggcgtc tgcaaccgga ccgtcgtggc tgtcgatgag cagcagatgg
     5221 ccgtcatcat cggtctgctc ggcatgaccg gcgagatgaa tctccgcgac gtcggcaagg
     5281 ggaaaatcgg tgagatagtc gagcgcggag aaaccgtgat ttgtggcgga cacaaggaca
     5341 ttattgatgt cgagcagcag gccgcagccg gtgcgcgcca cgatggcgcg aatgaagtcg
     5401 gtctcgctca tcgaggactc gcggaaggcg acgtaggtcg atggattttc cagcagcagc
     5461 ggacggcgga tcgcttcctg cacctcgtcg atgtgatcgc agacgcaggc cagcgtcgct
     5521 tcggtatagg gtagtggcag caggtcgttg aagaagctgg tctcgtgcga cgaccatgcc
     5581 agatgttcgg agaccagcgc cggctgatag cgggcgacaa ggttgcggaa gcgcgccaga
     5641 tgcgccttgt cgagcggttg cggcccgccg atggacatgc agacgccgtg cagcgacagc
     5701 ggatggtcac ggcggatcgc ttccagcgcg cgatgcggcg ggccgcccgc gcccatgtag
     5761 ttctcggcgt ggacttcgaa gaaccctcgg cgcggttccg ccgcaaggat tgccgggaga
     5821 tgttctggct tgaaactcgt cccggcaacg ccgccaatgg gagcgggaaa gcgaatcggc
     5881 accgtcgcag gtctgtcttg gttgatcgcc gtactcatcg cgtttctccg ctcatttcgg
     5941 tgacaacgtt cccggtgtta caccggcttc agcgagccct tcttgccgcc cggcagatcg
     6001 atgctggtgc aggtaccgcc ctgaacgaat ttccaggcgt tgccctggaa gtcggtggtc
     6061 gaggtgccct ggcaggtcgt acccgggccg gcggcgcaat cgttctggcc cttcagcgcg
     6121 acgccgaagc acttttcctt cttggcggca acggcggcat cagcttcggc cttggtcagc
     6181 ggggccgcag aagccagcgt cgcgagcgcg gtcgacatgg cgccggcgag ggcgagcgtg
     6241 gtgatggcgt gtttggcaga catcagagtt ctcctttttc agatgcatcg cctggcaggc
     6301 gaagggcgca tggattcgtt cgttcggcgc gcaggcggcg ttaccgccgg gttgctcacg
     6361 aatccgtgag accgattggg tcgggcgcac gggtggtgcg cctgggccgg gattagcggg
     6421 caagcgccgg ctcaaatcca atgccttggc gctatcgaga cgttagctcc acggcattgg
     6481 cgaggattgc ccgcatttgc cagacgtaac gaatgacggc cggcggcgta gagcgatgtt
     6541 agaggatgcg caggtccgcg aaggcgaaaa cggcgatgaa tgttgatccc ggacggcacc
     6601 ttcacgcgat cgcaaggctg accgtcagca ttcgcctcgc cgtgtccgcg ctggtcctgg
     6661 ctgcaatcct gctgacggcg gccctctcca gcctgttgtg gtggcgcacg gcggaggcga
     6721 ccagctggca gctcgcctcc accatcaacg agcagatcgt ggcggcggtg cgcaaggagg
     6781 tcgcggccat cgtcgacgag gcgcgcgccg cgcataccgc gatccgcaca ttgttcctgc
     6841 aaaacgtgct cgatacccgc gaggccgaca agcgcgagtt cgttttcctg tcgcaactgc
     6901 agtcgcaggc gacgatctcc tgggtcgcct tcggctggcc tgacggctcg ttcttcgccg
     6961 cgcacaagct cggcgaccgc cggctggaga tgatggagat ctcgttggcc gatcatcccg
     7021 gccaccgccg cgtcgacgag tatgacgtgg tgcccggcga tatcgaattc gccaatcggc
     7081 gcttcgagcc gaccgatttc cgcgtggccg atcaggcctg gttcaaggcc gggctcagcg
     7141 cggacgatcc gcaatggttc agggtcatgg accatccgac cggcgcgcga ccctcgattg
     7201 ccttcgtggg cccgatcgac gtctaccagg agaggcaggg cgttctggcc gtcgtcatcg
     7261 aatacacgcg gcttgcgcgc ttcctgtcgc agcttgaagt cgggcgtaca ggaacggcgt
     7321 tcatcgtcga tgacggcggc gagctcatcg cagcgccgga caaggaggcc gacgagctgc
     7381 atccggcgcg cggcgacacc acgctgctgc cgctcgcgcg cgtggcgctc gaaaaagccg
     7441 gcgaagccgg ccgcaaggag gcctggcgca gccggctcaa ttcggacggc ggggcctacg
     7501 aggtcgcact gacgccgttg ccgtttcccg gctggtcgct tgcgaccgtg atccccgagg
     7561 ccgagtttct cggcccggtc gagacgacgc tgcgacgcct gatcctcgga ttggccgtcg
     7621 gcgccgtgct cgctgcgctg gcttcggcga tgctggcgcg ctccgtcatc gccgcgccgc
     7681 tgtcgcgcgt cgtcggcgag cttcgtcatg tcgaggcgtt tgcgctggag caggttcgcc
     7741 ggcatccgtc gcgcctcaag gagatcgcga gcctgtctgg cgccatcgcc gagatggcct
     7801 ccggcctctc cgcgttccgc aagttcattc cagccgatct ggtccgtgcg ctgctgcgcc
     7861 agggcgtcga ggcaaggcct ggcggcagca tccaggaact gagcgtgatg ttcatcgaca
     7921 tcgccggatt caccgggctg tccgagcggc tcggcgatcg cgtcgtgccg ctgctgtcgc
     7981 gctatctcga cgttacctcg gaaataatcg tcgccaatgg cggcaccatc gacaagttca
     8041 tcggcgacgc cgtgatggcg ttctggggtg cgccgcagcc gcaggaggat cacgccgcgc
     8101 ggtgctgccg tgccgcgctc gcgtgctgca gggcgatcga ggcctccggc ctcgccgacg
     8161 acctcggcca gccgctccag atcaggatcg gaatcaattc cggccgcatg ctggttggca
     8221 atatcggttc ggaactgcgg ctgaactaca ccgtgatcgg cgatgccgtg aacgtcgcaa
     8281 gccgtctcga aggcgcgaac aaatcctatg gaacgcaaat cctgatcggc gagagtaccg
     8341 aacgtctggc gcgcggcgcc gtcgtgacgc gcgaggtcga cagcatcgcg gtctacggac
     8401 gggaggaggg gctcgccgtc cacgagctga tcgggattgt cgacgaagat gccggcatgg
     8461 caggctggat cgcgcgctac gaacagggat tggccaatta tcgcgcgcga cgctttgcgg
     8521 tggcgctcgc cgatttcgaa gcggtgctcg ccgagcgcgg tcatgatcgc ccgtccgaac
     8581 tcatgcgcga gcgttgccgg caatttgcgg cgacgatgcc cgatgcagca tggcagccgg
     8641 ttgtggcgct gaggacgaaa tagcggatca atctgccggg caaattttgc cagggggtac
     8701 cagccgcgag agggtccggg agagaaagcc gatgcaaatc aatccagaat cgatggcaca
     8761 taaattgctc atcgttccac gcaatctcga ccgcacacag gaattcgagc cgtgaccaac
     8821 gtcagctata ccgtcccgcc gaactggacc gcaggctccg acaagacggg cgcgagcaaa
     8881 aacaagaatc cgaacgatcc gaacatgctg acgacgggcg cctatggcag cgcgttcgag
     8941 ctgttgctcg acaatcgaac caaccgcgac ggcaccacca cggagacgct gacctacaag
     9001 agctacagcg gcaagaccaa cggcacgttc tcggcgacgg ccgagacggg gacgggagcc
     9061 gatctcggca gtgcctacca ggcgctgctc gccacgcttc aggccaatgg cgagatggac
     9121 gacaagaccg ccgcgcagct cgacggcgcc atgggcaagc tcgacaccca atccgagagc
     9181 ggcgccaagg atgccgaagt gtccggcggc ggcctgctgg tgcaggcggg tggcccggtc
     9241 tatttcagcg gcatctcggc ctatcagaac cagttcgtgg attcgctcgt caacgagctg
     9301 agcacgaggc tgaacctcga cgccaaggcg tgacggcaat tcatcgctgg tgaaaccggt
     9361 tggcaaagcc ggcccaaccc gcgcatgatg cgtgggcgat ctcggccaca tgctttcccg
     9421 gataaattca agacatgaaa accacgctgc tgaagtccga agactacacc cgctcgccct
     9481 ggaagaacgg cggcggcatc ttcaccgaca tcgcggatgc ccatcgcgcc gacgcgcagg
     9541 cgaaggattg ggacagcctg ctctggcgct ttgcttccac accgatcgtg gcgcccggtc
     9601 ccttctccta catgcccggc atcgaccgcc tgcaaatggt cgtcggtggg cgcgggctgg
     9661 tgctgaagtc gcccagccag gacttcgacg agcgtgaacc ttttacgacg gtgcgcttca
     9721 ctggcgagat ggagatcgtg accgagctcg aggcgggtcc cgtcgaggtc gtcaacctga
     9781 tggcccgccg cggcgcggcg gagatcgagc tgctggcact cagggaaccc ggcgagcggc
     9841 ctttgtccgc cggcacccac ctcgtttacg cggtttccgg cgattgcagc attcgtctca
     9901 atagcgagga tattgtcatc cccgccggca gcacgctgaa ggtcgagctg tccgtggcgt
     9961 ccacgctggc gcttgtctcg gggctggctg tgctggcttc gattcagctc gtcggctagg
    10021 cgcatctccc gcgttcggcg agcatcctgc ctgtcagcct catgccttca gcgcgtcgat
    10081 cacggcgcgc aggcccgcgg tctgatgcgc ccggctaggg aagtacagga acagatcctc
    10141 gtcgaccggg caccagtcct gcaggcactt caccagcttt ccctcgcgaa gagcgggtgc
    10201 ggcgcggtgc tcccagacat aggcaaggcc gaggccgcgt gctgcggcat cgaccatcag
    10261 ctcctggcta tcgaccgtga gcgggccgtc caccgacatc tcgatcgtct cgccgttctg
    10321 ctcgaacacc cagtgcagca ccgttccgct cggaaatgcg tagcggatgc agacgtgatc
    10381 ctgcagttcc tgaggcgtcg tcggcgccgc ccgctcttcg aaataggcgg gcgcgccgac
    10441 cacggtgaaa cgcaagttgg agcggatgcg caccgcgatc atgtcctgcg cgagccgctc
    10501 tccgaggcgg atgccggcgt caaagccctc ttcgacgatg tcgacgcgcc gatcggttgc
    10561 tgcgatctcc agccggaggc caggattggc cttcagcagc gtctcgatcg catccccgag
    10621 cacgaacggc gcaattgtat tgggcacgtt gaggcggacc gtgccgaacg gcgtattgcg
    10681 ccacgaattc aattcgtcga cggcgacgcc gatgtccgtc agcgccgggc cgaggcgcgc
    10741 gaacagcgtc tggcccgcat cggtcaggga gacgttgcgc gtggtgcgat gaatcaagcg
    10801 tacgccgacg cgatcctcca ggctgcgcac ggcatggctc atcgcggagg cagacgtgcc
    10861 ccgctcgacc gcggccttgc ggaagctgcg atgggtggcg accgcggcga aggcggcc
//
