LOCUS       NC_010694.1            10777 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NC_010694.1 Erwinia tasmaniensis Et1/99, complete sequence.
ACCESSION   NC_010694
VERSION     NC_010694.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:38:28
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 2716948
            Orig. end    :: 2727725
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10777
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[2721948:2722725](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5777)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..10777
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10777
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     CDS             complement(466..1650)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124:acetyl-CoA acetyltransferase (Score: 483.9;
                     E-value: 4.8e-147)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1_2421"
                     /transl_table=11
                     /translation="MNEVVVVSARRTATGAFMGGLSGLSAVALGSHVIRALLEESGVKA
                     SEISQVIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSIHLGMQALLA
                     GEATFVIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDIHMGVTAEN
                     VADKFGITREMQDEFALLSHMKAIRAQEQDVFEREIVPVEWQNRKGETQRVDTDEGPRQ
                     TSIEKLEKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTRCTAEAQGLPVLASLGEFTNS
                     GIKPALMGAGPIQAITDCLTRSGWKPEDIEHLESNEAFAAQALAVIAQTGISPERINPY
                     GGAIALGHAIGSSGCRILVTLVHGLIRTNGQRGMAALCIGGGEGVALSVYRQKS"
     PFAM_domain     complement(484..843)
                     /aSDomain="Thiolase_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02803.20"
                     /description="Thiolase, C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2421_0053"
                     /evalue="8.30E-40"
                     /label="ctg1_2421"
                     /locus_tag="ctg1_2421"
                     /protein_end="389"
                     /protein_start="269"
                     /score="135.4"
                     /tool="antismash"
                     /translation="PVLASLGEFTNSGIKPALMGAGPIQAITDCLTRSGWKPEDIEHLE
                     SNEAFAAQALAVIAQTGISPERINPYGGAIALGHAIGSSGCRILVTLVHGLIRTNGQRG
                     MAALCIGGGEGVALSV"
     aSDomain        complement(487..1635)
                     /aSDomain="TIGR01930"
                     /aSTool="tigrfam"
                     /description="AcCoA-C-Actrans: acetyl-CoA
                     C-acyltransferase"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2421_0018"
                     /evalue="1.70E-129"
                     /identifier="TIGR01930"
                     /label="ctg1_2421"
                     /locus_tag="ctg1_2421"
                     /protein_end="388"
                     /protein_start="5"
                     /score="430.1"
                     /tool="antismash"
                     /translation="VVSARRTATGAFMGGLSGLSAVALGSHVIRALLEESGVKASEISQ
                     VIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSIHLGMQALLAGEATF
                     VIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDIHMGVTAENVADKF
                     GITREMQDEFALLSHMKAIRAQEQDVFEREIVPVEWQNRKGETQRVDTDEGPRQTSIEK
                     LEKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTRCTAEAQGLPVLASLGEFTNSGIKPA
                     LMGAGPIQAITDCLTRSGWKPEDIEHLESNEAFAAQALAVIAQTGISPERINPYGGAIA
                     LGHAIGSSGCRILVTLVHGLIRTNGQRGMAALCIGGGEGVALS"
     PFAM_domain     complement(868..1641)
                     /aSDomain="Thiolase_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00108.25"
                     /description="Thiolase, N-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2421_0052"
                     /evalue="4.50E-98"
                     /label="ctg1_2421"
                     /locus_tag="ctg1_2421"
                     /protein_end="261"
                     /protein_start="3"
                     /score="327.9"
                     /tool="antismash"
                     /translation="VVVVSARRTATGAFMGGLSGLSAVALGSHVIRALLEESGVKASEI
                     SQVIMGQVLTAGCGQNPARQSALKAGLPVETQCMTINKVCGSGLKSIHLGMQALLAGEA
                     TFVIAGGQESMSSAPHILTSSRTGTRLGNSEMKDSLVSDGLWDAFDDIHMGVTAENVAD
                     KFGITREMQDEFALLSHMKAIRAQEQDVFEREIVPVEWQNRKGETQRVDTDEGPRQTSI
                     EKLEKLKPVFRKGGTVTAGNASSLNDGAAAVLLCTR"
     CDS             complement(1647..2060)
                     /locus_tag="ctg1_2422"
                     /transl_table=11
                     /translation="MSEASDLTRYGKDIMDRLEPGLADKVTGRLAELDPSLPTLITDYA
                     FGAVVGRPGLDLKTREMLTVASLVTLGNAMPQLELHMRAALNTGVTPEELLEIVIQMAV
                     YAGVPACMNGLTAYRAAMAATGHTLPGLKGKNA"
     PFAM_domain     complement(1710..1958)
                     /aSDomain="CMD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02627.22"
                     /description="Carboxymuconolactone decarboxylase family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2422_0054"
                     /evalue="7.00E-19"
                     /label="ctg1_2422"
                     /locus_tag="ctg1_2422"
                     /protein_end="117"
                     /protein_start="34"
                     /score="67.8"
                     /tool="antismash"
                     /translation="PSLPTLITDYAFGAVVGRPGLDLKTREMLTVASLVTLGNAMPQLE
                     LHMRAALNTGVTPEELLEIVIQMAVYAGVPACMNGLTA"
     CDS             complement(2053..3267)
                     /gene_functions="transport (smcogs) SMCOG1184:major
                     facilitator transporter (Score: 288.3; E-value: 1.2e-87)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_2423"
                     /transl_table=11
                     /translation="MNAESSQQDMTIRSWLALFILALSTFTIVTTELAPVGLLTPIAKG
                     LNASESAVGMTVSLYAWVGALSALFASVFLGNVAKKRLMLTLTVILFLSNVLAATVSTY
                     TVLLAARVLGALAHGAFWAMIGATAVSIVPARYIGAATSIVFGGVSAASVFGVPVSNYI
                     GIHFGWRQAFWLMAVLSVVAFAGISVLIPEITKKSAIGFGALKGVLKSSALWKIYSATL
                     LAVTAHFAAFTYIEPWLHTQHSLATSVIPAVLFVYGIAGLAGNFLTGVVIDKYLKATVS
                     ISVLLICAVLVFLGTSGAALSGIAVFIAMGIWGVAVSGIFVGFQTWVLRLAGDKTFPAS
                     AVYVSFFNTAIGIGASAGAWMVSEFSVPMLYVVAGVAIGLSVLLVAAIPTRVASAQNVL
                     EKSYE"
     PFAM_domain     complement(2269..3222)
                     /aSDomain="MFS_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07690.18"
                     /description="Major Facilitator Superfamily"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2423_0055"
                     /evalue="7.80E-29"
                     /label="ctg1_2423"
                     /locus_tag="ctg1_2423"
                     /protein_end="333"
                     /protein_start="15"
                     /score="100.8"
                     /tool="antismash"
                     /translation="LALFILALSTFTIVTTELAPVGLLTPIAKGLNASESAVGMTVSLY
                     AWVGALSALFASVFLGNVAKKRLMLTLTVILFLSNVLAATVSTYTVLLAARVLGALAHG
                     AFWAMIGATAVSIVPARYIGAATSIVFGGVSAASVFGVPVSNYIGIHFGWRQAFWLMAV
                     LSVVAFAGISVLIPEITKKSAIGFGALKGVLKSSALWKIYSATLLAVTAHFAAFTYIEP
                     WLHTQHSLATSVIPAVLFVYGIAGLAGNFLTGVVIDKYLKATVSISVLLICAVLVFLGT
                     SGAALSGIAVFIAMGIWGVAVSGIFVGFQTWVLRLAG"
     CDS             complement(3288..3938)
                     /locus_tag="ctg1_2424"
                     /transl_table=11
                     /translation="LQKSAAFFDVDETLITMKSMFDFYGFWCRENNEMSALERYMTQFK
                     EEVKKGKCREYLNREYYRQFSGVSYKKLEEAGEKWFRSKSVKTLFINSAVAALRQHQAE
                     NRLTIFVSGSMRPVLAPVARYLGVTEILCAPLELTESGTLTGEIGTPQTIGGGKREALI
                     AFCRGKEISPEDCYAYGDDLSDIPMLESVGHPICVGKKSALASHAIHQHWPVI"
     aSDomain        complement(3339..3926)
                     /aSDomain="TIGR01490"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB-hyp1: HAD hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2424_0019"
                     /evalue="5.30E-39"
                     /identifier="TIGR01490"
                     /label="ctg1_2424"
                     /locus_tag="ctg1_2424"
                     /protein_end="200"
                     /protein_start="4"
                     /score="132.5"
                     /tool="antismash"
                     /translation="AAFFDVDETLITMKSMFDFYGFWCRENNEMSALERYMTQFKEEVK
                     KGKCREYLNREYYRQFSGVSYKKLEEAGEKWFRSKSVKTLFINSAVAALRQHQAENRLT
                     IFVSGSMRPVLAPVARYLGVTEILCAPLELTESGTLTGEIGTPQTIGGGKREALIAFCR
                     GKEISPEDCYAYGDDLSDIPMLESVGHPICVGK"
     aSDomain        complement(3363..3926)
                     /aSDomain="TIGR01488"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IB: HAD phosphoserine phosphatase-like
                     hydrolase, family IB"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_2424_0020"
                     /evalue="4.30E-26"
                     /identifier="TIGR01488"
                     /label="ctg1_2424"
                     /locus_tag="ctg1_2424"
                     /protein_end="192"
                     /protein_start="4"
                     /score="89.9"
                     /tool="antismash"
                     /translation="AAFFDVDETLITMKSMFDFYGFWCRENNEMSALERYMTQFKEEVK
                     KGKCREYLNREYYRQFSGVSYKKLEEAGEKWFRSKSVKTLFINSAVAALRQHQAENRLT
                     IFVSGSMRPVLAPVARYLGVTEILCAPLELTESGTLTGEIGTPQTIGGGKREALIAFCR
                     GKEISPEDCYAYGDDLSDIPMLESV"
     PFAM_domain     complement(3369..3923)
                     /aSDomain="HAD"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12710.9"
                     /description="haloacid dehalogenase-like hydrolase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2424_0056"
                     /evalue="8.80E-25"
                     /label="ctg1_2424"
                     /locus_tag="ctg1_2424"
                     /protein_end="190"
                     /protein_start="5"
                     /score="88.4"
                     /tool="antismash"
                     /translation="AFFDVDETLITMKSMFDFYGFWCRENNEMSALERYMTQFKEEVKK
                     GKCREYLNREYYRQFSGVSYKKLEEAGEKWFRSKSVKTLFINSAVAALRQHQAENRLTI
                     FVSGSMRPVLAPVARYLGVTEILCAPLELTESGTLTGEIGTPQTIGGGKREALIAFCRG
                     KEISPEDCYAYGDDLSDIPMLE"
     CDS             complement(3942..4166)
                     /locus_tag="ctg1_2425"
                     /transl_table=11
                     /translation="MPFVHITTWPAKDEGDMLRLHEDITYAVHKNTGAPLDKISVVISE
                     IQPSRWSDAGIPGDDKDFPVKSRRKSYEE"
     PFAM_domain     complement(4014..4163)
                     /aSDomain="Tautomerase"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01361.23"
                     /description="Tautomerase enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2425_0057"
                     /evalue="7.70E-06"
                     /label="ctg1_2425"
                     /locus_tag="ctg1_2425"
                     /protein_end="51"
                     /protein_start="1"
                     /score="25.8"
                     /tool="antismash"
                     /translation="PFVHITTWPAKDEGDMLRLHEDITYAVHKNTGAPLDKISVVISEI
                     QPSRW"
     CDS             complement(4175..5008)
                     /locus_tag="ctg1_2426"
                     /transl_table=11
                     /translation="MPELSSQKYRLTATTQGPLYPPAEVMDQDGNFVVVGMVPGDNGLA
                     WRRVIVAADSPLPGFGDTAPYKILRDIDCMSQAELKDVILHTLPLPVPLNNYGMLFAPE
                     QRPQANTERRPSLPLHEGYIADYRSSDGKRQIPPVTLEKWLRAEGSLEVCLSENSRRAL
                     FRFTFRNLVPDSVYTVMSLREQDLATDNPSRPGPLGIPNVFITDHDGNANYWAELPDPF
                     PAHERNGNRVINVVVLYMSSRQSYGGAIGLYGLGGDIHAHLKLKSRSFDELITVK"
     CDS             complement(5001..5777)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_2427"
                     /sec_met_domain="AfsA (E-value: 2.9e-10, bitscore: 37.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKKLVVVGDKFNEFANGKDVLTITQLELLTQIPANIIDKEHEI
                     IIGQGVRKDFAGKVISNQPRNTIHGNKLKMCSLEKLVNDKKNEYCHKRLEHNVLIGPAE
                     QIEQNSNLFAMPLLIDERCELMADHQTGQHIQGMLLVEASRQAFIAITEEFTYKQETGR
                     YYVINSMAINFSSFLFPLPAQVHFEYLEKDVNERRGRFKAQVRVMQHKTLCASMDVSFT
                     VYPSGLISEKEKSLSEMAMQAMIVEHQGTTLKVTHA"
     PFAM_domain     complement(5112..5507)
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2427_0058"
                     /evalue="2.00E-13"
                     /label="ctg1_2427"
                     /locus_tag="ctg1_2427"
                     /protein_end="222"
                     /protein_start="90"
                     /score="50.6"
                     /tool="antismash"
                     /translation="HKRLEHNVLIGPAEQIEQNSNLFAMPLLIDERCELMADHQTGQHI
                     QGMLLVEASRQAFIAITEEFTYKQETGRYYVINSMAINFSSFLFPLPAQVHFEYLEKDV
                     NERRGRFKAQVRVMQHKTLCASMDVSFT"
     CDS             complement(5788..6399)
                     /locus_tag="ctg1_2428"
                     /transl_table=11
                     /translation="MSSNYVLVSRFPLKNTVSVDAFSSLKSTESTRYYFCEEQGVNELL
                     ELRSFNDIKELSWVEEEIGSMFYQFADVISADIRRELLKFVESPIDSKKNLPETQYIQL
                     RHVEVPAHNYQQYRQWRDETIFNVVRDNKDKIESFEAFHSLISGVPGVMFISAFNGDKE
                     TYKEAFTNARYQEIIQQAGDNYITGGNEGLYTRIYRACCC"
     CDS             6591..7517
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 146; E-value: 2.3e-44)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg1_2429"
                     /transl_table=11
                     /translation="MAIAENLISNNLPSVKQLQCFLAVAHELNFRRAAERLRMTQPPLT
                     RQIKCLEAVLEIQLFSRSTHEVSLTEAGRELVIRAEKLLQELSEMKMVAPSSKKILRIG
                     LTRTLNFELIESINEQLNKLDVSNDVETPNLTSAQLLHCLSKNTLDLVLTGEKGTGHEN
                     IVQFRWVYREPLLMAMPAMHPASLKEKVSLEDIRDLPLFWFARSANPAFYDKCESYFDR
                     LRFPLKRVKEPDDSLVMLTHIARGKGVALMPQSKCTFNQEGLCYRELTDHESSKLNIDV
                     YAATRLNEKRENILNAQDALCRNNLSE"
     PFAM_domain     6636..6812
                     /aSDomain="HTH_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00126.29"
                     /description="Bacterial regulatory helix-turn-helix
                     protein, lysR family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2429_0059"
                     /evalue="1.00E-18"
                     /label="ctg1_2429"
                     /locus_tag="ctg1_2429"
                     /protein_end="74"
                     /protein_start="15"
                     /score="67.1"
                     /tool="antismash"
                     /translation="KQLQCFLAVAHELNFRRAAERLRMTQPPLTRQIKCLEAVLEIQLF
                     SRSTHEVSLTEAGR"
     PFAM_domain     6888..7454
                     /aSDomain="LysR_substrate"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03466.22"
                     /description="LysR substrate binding domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_2429_0060"
                     /evalue="7.30E-16"
                     /label="ctg1_2429"
                     /locus_tag="ctg1_2429"
                     /protein_end="288"
                     /protein_start="99"
                     /score="58.3"
                     /tool="antismash"
                     /translation="ILRIGLTRTLNFELIESINEQLNKLDVSNDVETPNLTSAQLLHCL
                     SKNTLDLVLTGEKGTGHENIVQFRWVYREPLLMAMPAMHPASLKEKVSLEDIRDLPLFW
                     FARSANPAFYDKCESYFDRLRFPLKRVKEPDDSLVMLTHIARGKGVALMPQSKCTFNQE
                     GLCYRELTDHESSKLNIDVYAATRLN"
     CDS             8046..8345
                     /locus_tag="ctg1_2430"
                     /transl_table=11
                     /translation="MAERYPAYGFGLMFRKLPQSGRGGMRAVLTGVSPAKAKLPPQGQK
                     RLPNRHPQPLTVPVNTNHCWSVDFMSDALTDGRRFRLFNVVEDFNREALAIDVD"
     CDS             8513..8608
                     /locus_tag="ctg1_2431"
                     /transl_table=11
                     /translation="MQNGFIERFNKMLRTEILDMYFSALCQNFAF"
     CDS             complement(8842..9981)
                     /locus_tag="ctg1_2432"
                     /transl_table=11
                     /translation="MKKTILACFVSVFLLTGCGPKELSSEDKQKVDQLKAELSQTESEI
                     SSANVTNQNYSGGLVKTLISARVEILKTNQALIKQRIDAIESGAKITMDVPGAKPDEAT
                     SEAIKREMDSLNAQISQAKAEANQYSGGLVLAMKLAAIATQEQTLAMLQQRYLSAKYGL
                     ANAVPSDLKQGNFAEAKQNEKSAQASNTRPLLPPGEGPFGLESGLTKKNIEDMTGEELK
                     PVENVPNLYMSQSLPKKNAEFEVYGLMISPKSGLCEIKALGKRIDTDSHGLALRSKYNE
                     LSDSLSSIYGKPENTDFLLSGSIWKEPQDWMMGLNKNERFLSTRWKAAPTAPFKNNLKS
                     VFMEARAKGPSEVYIFLQYDFNNIGACNAEIEEAKKSYL"
     CDS             complement(10025..10381)
                     /locus_tag="ctg1_2433"
                     /transl_table=11
                     /translation="MNKSVALAAVVMLLAGCSSRVADLTVASTKNYNLNSNNFVKGARV
                     KAEDSAPVVLFPLGIPNVKTAIDRAIEKNRCSVALSDVVVTQFNHSFLFGKFGFIIEGT
                     EIIDRSQSGCENAS"
ORIGIN
        1 ttgctctggt tccctactcc cctctgggaa gaggcttcct gaccggtaaa tatcgtagta
       61 ataatgattt tgcagaaggt gacttccgca aaaacaatga gcggttcatc cagtcgagcc
      121 tggatcacaa tgctaagctg cttgacgtta tcgccccgct cgcggaaaaa tacggctgca
      181 ctacgggtca gattgcgctg gcctggttgc ttgcacagta tgaaaagctt gtgcctatcc
      241 caggaaccaa acacatcagt tacctgaccg aaaatgcaca ggcggccaac attgttctgg
      301 aagaaaccga tatcatcctg ctgaacaaca ttcatctgca cgttgagata aaaggtggcc
      361 gctactctga ggaaggcatg aaaggcgtta acgcctgagt actgaactca taaaaaaaac
      421 cgggcaatgt ccggtttttt tatttctgga taaggcggct gccccttagc ttttctgtcg
      481 gtagacgctc aacgcgacgc cttcgccacc gccaatacag agtgcagcca tgccgcgctg
      541 accgttggtc cgtatcagac cgtgtacaag agtgaccagt atacggcatc ccgacgatcc
      601 aatagcatga ccaagcgcga tagcaccacc gtaagggttg atgcgctctg gtgaaatgcc
      661 tgtctgagcg ataactgcga gagcctgagc cgcaaacgct tcatttgatt cgagatgttc
      721 aatgtcttca ggcttccagc ctgaacgagt aaggcagtct gtgattgcct gtatcggacc
      781 ggcacccatt agtgccggct tgatacccga gttggtgaac tctcccaaag aagccagaac
      841 cggcagtccc tgtgcctctg ctgtacatcg ggtgcacaga agaacagcgg cggcaccgtc
      901 gttaagtgag gaggcattac ccgcagtgac tgtgcccccc tttctgaata caggtttgag
      961 tttctcgagc ttttcgatgc tggtctgacg cggaccttca tcagtatcaa ccctctgagt
     1021 ttcacccttt ctgttctgcc actctacagg aacaatttcg cgctcaaaca cgtcctgctc
     1081 ctgcgccctg attgccttca tatgagataa aagcgcgaat tcgtcctgca tttcacgagt
     1141 gataccaaac ttatccgcca cgttctcagc agtaaccccc atgtggatat catcaaaagc
     1201 atcccagagc ccatcagata ccaggctgtc tttcatctcg ctgtttccga gacgtgtccc
     1261 agtacgcgac gatgtcagga tatgcggtgc actggacatg ctttcctgac cgccagcaat
     1321 cacaaacgtt gcttctccgg cgagcagtgc ctgcataccc agatgaatcg atttcagacc
     1381 cgaaccgcag actttgttaa tggtcataca ctgtgtttcg acaggcagtc cggctttcag
     1441 tgcagactgg cgtgcggggt tttgaccgca gccggcggtc aatacctgtc ccataatgac
     1501 ctgtgaaatc tcacttgctt tgacgccgga ctcttcaaga agcgcacgaa taacatgact
     1561 tcccagcgca acggcggaga gcccggacag tccccccata aatgcaccgg ttgccgtgcg
     1621 tcttgcagaa acaaccacga cttcattcat gcgttttttc ctttcagacc gggtagtgta
     1681 tgcccggttg ccgccattgc ggcacgatag gcggtcaggc cattcataca ggccggaacg
     1741 cctgcataaa ccgccatctg aatcacgatt tccagtaact cctcaggcgt gacgccggta
     1801 ttaagcgcag ctctcatatg cagctccaac tgcggcatag cattacccag cgtgacaagc
     1861 gatgccactg tcagcatttc acgggtcttg aggtcaaggc cagggcgtcc caccacagca
     1921 ccaaacgcgt agtcggtgat cagggtcggt aatgaaggat caagttcagc caggcgtcct
     1981 gtgactttat cggcaagacc gggttcaagg cggtccataa tgtcttttcc ataacgggtc
     2041 agatcgctag cttcactcat aagatttctc caggacgttt tgtgcagacg ccacgcgcgt
     2101 cgggatagcc gccacgagca gaactgagag tccgatggcc acgccggcta cgacgtagag
     2161 catcggaaca gagaattcgg ataccatcca cgcaccggca gaggcgccga taccgatagc
     2221 agtgttgaaa aatgaaacgt aaaccgcaga agcagggaag gttttatctc cagccaggcg
     2281 caggacccac gtctggaagc ccacaaagat cccggagaca gccacacccc agatacccat
     2341 tgcgatgaag acagcaatgc ctgaaagcgc tgccccggat gttcccagga ataccagcac
     2401 cgcgcaaatg agcaacactg aaatggaaac tgtcgctttg agatatttat caatcaccac
     2461 gccggtcagg aaattgccgg caagtcccgc aataccgtac acaaacagca cagcaggaat
     2521 aacagatgtg gccagtgaat gctgcgtgtg cagccagggc tcaatgtacg taaaggcagc
     2581 aaagtgcgcg gtgacagcga gcagcgtagc agaatagatt ttccacagcg ccgaagattt
     2641 cagtacgcct ttaagcgccc caaagccaat cgcgcttttt ttggtgatct ctggtatcag
     2701 cacgctgata ccggcaaatg ccaccacgct gagtaccgcc atcagccaga atgcctgtcg
     2761 ccagccgaag tgaatgccaa tatagtttga aaccggcacg ccaaacacac tggctgccga
     2821 tacgccacca aatacgattg aggttgcggc accaatatac cttgccggca caatggaaac
     2881 ggccgttgct ccaatcattg cccagaatgc accgtgagcc agcgctccca gcacgcgtgc
     2941 agccagcagc accgtgtaag tgctcaccgt cgcggcaagt acgttagaca ggaaaaggat
     3001 cacggtgagc gtaagcatca gccgcttctt ggccacattg cccaaaaata ctgatgcaaa
     3061 aagtgcgctc agtgcaccta cccatgcata cagtgagacc gtcataccca ctgctgattc
     3121 agacgcattc aggccttttg cgattggcgt cagcaggcca acaggtgcca gctctgtagt
     3181 gacgatcgta aacgttgaaa gagccagaat aaaaagggca agccacgacc ttatggtcat
     3241 atcctgctga gagctttctg cgttcatatt tatacctcta aaatgattta aataacgggc
     3301 cagtgctggt gaatcgcatg gctggcaagc gctgatttct ttcctacgca aatcgggtgg
     3361 ccgacggatt ccagcatggg aatatcggag agatcgtccc cataggcata gcaatcttca
     3421 ggactgattt cttttccgcg acagaacgca ataagtgcct ctcgcttgcc accaccaatg
     3481 gtctgcggcg tgcctatttc acccgtcagt gttcctgact ctgtgagttc cagaggggca
     3541 cagagaatct ccgtgacacc caggtatcgg gcaaccggcg caagtacagg acgcattgac
     3601 cctgaaacaa agatagtcag tctgttttcc gcctgatgct gcctaagggc tgcgacggca
     3661 ctgttgataa atagagtttt gactgacttt gaacggaacc atttttcacc cgcctcctcc
     3721 agttttttat aactgacccc agagaactgt cggtaatact ctctgttcaa atactcgcga
     3781 cacttccctt ttttaacttc ttccttaaac tgcgtcatat aacgttcaag cgcgctcatt
     3841 tcattatttt cacgacacca gaatccgtaa aaatcaaaca tacttttcat cgttataagg
     3901 gtttcgtcga catcaaaaaa tgcggctgat ttctgcaaaa ttcactcctc ataactcttt
     3961 cttcggcttt ttaccggaaa atctttgtca tcgccgggaa tacctgcatc agaccagcgt
     4021 gaaggctgta tttcagaaat cactacagaa attttatcca gcggtgctcc ggtgttttta
     4081 tgcacggcat aagtaatgtc ttcatgtagc ctgagcatgt cgccttcgtc tttagcaggc
     4141 caggtcgtga tatgtacaaa tggcattttt tttctcactt tacggtaatt aattcatcga
     4201 atgagcggct tttcaatttc aaatgagcat ggatatcacc acccagccca tataatccaa
     4261 ttgcgccccc gtagctctgg cgggaactca tataaaggac aaccacgtta attacccgat
     4321 tgccattgcg ttcatgtgcc gggaacggat cgggtaattc cgcccaataa ttcgcattgc
     4381 catcgtggtc ggtgataaat acgttaggta tacccagcgg acccggacgg cttggattgt
     4441 ccgttgccag atcctgctca cgcaggctca ttacggtata aacactgtcg ggaaccaagt
     4501 tgcgaaatgt gaaccggaac agcgcacgtc gactgttttc tgacagacac acttcgagac
     4561 tgccttcagc gcgcaaccat ttttccagcg tgacaggcgg gatctgacgt ttaccgtcac
     4621 tgctgcggta atctgcgatg tatccttcat gcaaaggcag gctgggccgc ctttcggtat
     4681 tagcttgtgg ccgctgctct ggtgcgaaca gcatgccgta attattcagc ggtaccggca
     4741 gcgggagggt atgcaagatc acgtccttca gctctgcctg tgacatgcaa tcgatatcac
     4801 gcaatatttt gtagggtgcc gtgtctccaa aacccggaag cgggctatct gccgccacaa
     4861 tcactctgcg ccatgccaga ccgttgtctc ctggcaccat acctaccacg acaaagttac
     4921 catcctgatc cattacctcc gcaggggggt aaagcggccc ctgcgtggta gcagtcaaac
     4981 ggtatttctg actgcttaat tcaggcatgc gttaccttaa gggtcgtgcc ctgatgctct
     5041 actatcattg cctgcatagc catctcagac agcgatttct ccttctcaga aatcagccct
     5101 gaagggtata cggtgaagga gacgtccatg ctggcgcaaa gcgtcttatg ctgcattacg
     5161 cgcacctgcg ctttgaaacg accacggcgt tcgttaacgt ccttctcaag gtactcaaaa
     5221 tgaacctgcg ctggtaaagg aaaaaggaag ctcgaaaagt ttatcgccat actgttaatg
     5281 acgtaataac ggcccgtttc ctgcttgtag gtaaactctt cagtaatggc gataaacgcc
     5341 tgacggctag cttctaccag gagcattccc tgaatatgct gaccggtctg atgatcggcc
     5401 atgagttcac agcgctcatc gatcaacagc ggcatagcaa aaagattact gttctgctca
     5461 atctgttcag caggacctat cagaacattg tgctccagac gtttatggca atattcattc
     5521 tttttatcgt taacaagctt ttcaagagaa cacattttta gtttattgcc gtgaatagta
     5581 tttctcggct gatttgaaat cactttcccg gcaaaatctt tacgcacgcc ctgcccgata
     5641 ataatttcat gctctttatc gatgatgttt gcagggattt gcgttaacag ctccaactga
     5701 gtaatagtca ggacatcttt tccatttgcg aattcattga atttatcgcc gacaacaacc
     5761 agtttcttac agttcatggc gtactcctca gcaacaacaa gcgcgatata tacgggtata
     5821 gaggccttcg ttacctccgg taatatagtt atcacctgcc tgctgaatga tttcctggta
     5881 gcgtgcattg gtaaacgctt ctttataggt ttctttatcg ccgttgaaag cagaaatgaa
     5941 catcacacct ggcacgccac tgatgagcga gtggaaggct tcaaagcttt cgatcttgtc
     6001 tttattgtct cttacaacgt taaagatcgt ttcatcgcgc cactggcgat actgctgata
     6061 attgtgtgca ggaacttcaa catggcgcag ctgaatatat tgtgtctcag ggagattttt
     6121 cttactgtcg ataggtgact cgacaaattt cagcagctct ctgcgaatat ccgctgaaat
     6181 aacgtcagca aactgataga acatagatcc tatctcttct tccacccatg ataattcttt
     6241 aatgtcgtta aacgaacgaa gttccagcaa ttcatttaca ccctgctctt cacaaaaata
     6301 atagcgcgtg ctttcagtac tttttaatga cgaaaaagca tcaaccgaaa cagtattttt
     6361 taatgggaat cgagatacca gaacatagtt agatgacatt attatctccg gaaaaatgtt
     6421 ggattaggtt tcgtatgtaa gttatcaatc tgtgaattag atggaattgt taaatatgta
     6481 atataaattt tgtattactg caaacgagcc tttttctgaa atgagcttcc gtgttatatc
     6541 tgtacgtata aataagatag aatagtcttt cgattaacag ggtgaaattc atggcaattg
     6601 cagaaaattt gatcagcaac aacctcccca gcgttaagca gcttcagtgc tttctcgctg
     6661 tcgcacatga gctgaacttc cgcagagccg cagaacggct caggatgact caacctcccc
     6721 tgacccgtca gataaagtgt ctggaagctg ttttggagat acagctgttc agccgcagca
     6781 cgcacgaggt cagtctgaca gaggctggtc gcgaactggt tatcagggca gagaagctcc
     6841 ttcaggagct tagcgaaatg aaaatggttg caccttcctc caagaaaatt ctgcgtattg
     6901 gtctcacccg tacgctgaat tttgagctga tagaatctat taatgagcag cttaataaac
     6961 tggatgtcag caatgatgtt gaaacaccca acctgacatc ggcgcagctg cttcattgcc
     7021 tgtcaaaaaa cacgcttgat cttgtgttga ccggcgaaaa aggcacaggt catgaaaata
     7081 tcgttcagtt ccgctgggtc taccgcgaac cgctgttaat ggcgatgccg gctatgcatc
     7141 ctgctagcct gaaagaaaaa gtctcgctgg aagatatccg tgatttaccg ctcttctggt
     7201 ttgcaagaag cgccaatccc gccttctacg ataagtgtga aagctatttc gatcggctca
     7261 ggtttccgct taagcgggtc aaagagccag acgactcatt ggttatgctc actcacatcg
     7321 cccggggcaa gggggttgcc ctgatgccgc aatcaaagtg cacattcaat caggaagggc
     7381 tatgctaccg ggaactgacc gatcacgaat cgtcaaagct gaatatcgat gtctacgcgg
     7441 ctacccgact taatgaaaag cgagaaaaca tcctgaatgc tcaggacgcg ttatgtcgta
     7501 acaatttgtc agagtagagt tggggaagat cgcgacatat aaaaatcacc tccatgcgtc
     7561 tcgttcagcc accccgggtg atggtccatc cgtttattgc tgcggatgtc attatttgcc
     7621 gtgatgccat cgcccgcgtt caggtttctc tttaccgaaa tgctcccccc ggaggtgacg
     7681 tcagcgccag ccgaaagcgg cccggcaaag gcacccaccg ccctattccc gttccagatc
     7741 gccccccgtc cgcgtctgaa gctctctgac gcaacctttc tgacagtcat tccgtcacat
     7801 gcgcgcctgc ctgtgaatcg gcaaaacgca accggccgac gtgaagtccc cctgaatttc
     7861 gtcttaccgc ctgatagggt tctctgatta aaatacaaca aataaaggct catcatgaag
     7921 aaagcgcgtt tcactgagtc tcagatcctg cgggttctta agaggttgaa aggggcaggt
     7981 atgtgaaaga cgtctgccat gaaacgctgt ttcagaagtc agctattaca actggaaagc
     8041 aaaatatggc ggagcggtat ccggcgtatg gttttggcct gatgttcagg aagttacctc
     8101 agtcagggag ggggggaatg cgagcggttt taacgggtgt atcgcctgct aaagctaaac
     8161 ttccgccgca agggcaaaaa cgtttaccca atcgacatcc acagcctctt actgttccgg
     8221 ttaatacgaa tcattgctgg tcagttgatt ttatgagcga tgccctgacg gacgggcgga
     8281 ggttccgtct attcaacgta gtggaggatt tcaatcggga ggcactggca attgatgttg
     8341 attgaaacat accagctcat cgtgtcgttc gggttctgga acgtctgagc gaagaaagag
     8401 cttatccgat tgtattcgca gcaataacgg cccggagttc acagcggcga cgttaacaaa
     8461 atgggcagaa cttaacggca taatactcga ttttattcag tcggacaggc caatgcagaa
     8521 cggctttatc gaacggttta ataaaatgct gcgaacagaa atactggata tgtatttttc
     8581 cgctctctgt cagaatttcg ctttttaact gaacagtggc gtagtgaata taacgaagaa
     8641 cgcccgcaca gttcactggg tgggatgtct caagttattt acgcgaggaa aaagttggcc
     8701 ggagatccta actggaagtg gtactaaaaa acggagggac tacagtttga tggagtattt
     8761 tgctcataaa aaacctcgcc ggagcgaggt gtactaagca aatcaatcag taatgtatga
     8821 tgccgttttg attgtcaata tttataaata gcttttctta gcttcttcga tctctgcgtt
     8881 gcaggcgcct atgttattga agtcgtactg taggaaaata tagacctcac ttgggccctt
     8941 ggcccttgct tccataaata cagatttgag gttgttttta aaaggtgctg taggcgccgc
     9001 tttccatcga gtagagagga agcgctcatt tttgttcagc cccatcatcc agtcttgagg
     9061 ttctttccag attgagcccg aaagtaaaaa gtcggtgttt tcaggcttac catagattga
     9121 agacagagaa tctgagagct cattgtattt tgatcgaaga gcgagaccat ggctgtctgt
     9181 atcaattctt ttacccaacg ctttaatttc acaaaggcca gatttaggag aaatcattag
     9241 gccataaact tcaaattcag cgtttttttt cgggagcgat tgtgacatgt aaaggttcgg
     9301 tacgttttca acaggcttaa gctcttcacc agtcatatct tcgatattct tttttgttaa
     9361 gcctgactca aggccgaatg gcccctcacc cggtgggagt aatggtcgag tgttgctagc
     9421 ctgcgctgac ttctcatttt gctttgcttc cgcaaagttg ccttgcttta aatcagacgg
     9481 cactgcgtta gcaagtccat acttggccga caaatagcgt tgttgaagca tcgccaacgt
     9541 ttgctcctga gttgcgattg ccgccagttt catcgcaaga accaagcctc cgctgtattg
     9601 attagcttct gctttggcct gagagatctg agcatttaac gaatccattt ctctcttaat
     9661 tgcctcagat gtcgcctcat caggctttgc ccccgggaca tccatggtga tctttgcgcc
     9721 tgactcaata gcatcaatcc gctgcttaat tagtgcttgg ttagtcttga gaatttcaac
     9781 tctggcgctt ataagagtct ttaccaaccc gccagagtag ttctgattgg tcacatttgc
     9841 agacgaaatc tcactttctg tttgtgataa ctccgccttc agttgatcca ctttttgttt
     9901 atcttcagaa gaaagctcct ttggcccaca ccctgtcaaa agaaatacag atacaaaaca
     9961 tgccaaaata gtctttttca tttccttgcc tcatagcaaa aaaccactca aaggtggcta
    10021 tcttttaact cgcgttttca caaccagact ggctacggtc aatgatctcg gtgccttcaa
    10081 taatgaagcc aaactttccg aataggaaag agtggttgaa ttgagtcact acaacatcgg
    10141 agagtgcgac tgaacagcga ttcttctcga ttgctctgtc tatagctgtt ttaacgttgg
    10201 gaatgccaag agggaagagc actactggtg cagagtcctc tgctttaaca cgcgcccctt
    10261 taacgaaatt attagagttc agattatagt ttttggtgct tgctacggtc aaatctgcca
    10321 cacgtgaact acatcctgct agcaacatga ctaccgcggc caatgccact gacttattca
    10381 tattttttcc ttcgattaca atcggaaaca tcctatcaat aatttttaac attgcaatta
    10441 ttgtatttcg tgctgtttgg cggctttgat ttcggtggcg gtgagcttcg tttgggaggt
    10501 ctcgctttca ttgaacctgc taatgccccc aagatgggtg tagctttcaa tagaaattag
    10561 taaacgttac gataatcctg agtccagtga gcatgagaaa atgagtagcg atttgagtga
    10621 tttactaccc gaatcgttat ctaagtgcct ttgaaactca cccagatcat ttactgaaag
    10681 atgaggaaat gtttttgttt tggtactttc agaacaccta ccagatctga tactggatta
    10741 cgctccgcca gaaatgactg cataactgaa gatttga
//
