LOCUS       c00068_NZ_JHYD..        1880 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JHYD01000080.1 Ruminiclostridium cellobioparum DSM 1351 = ATCC
            15832 T344DRAFT_scaffold00068.68_C, whole genome shotgun sequence.
ACCESSION   NZ_JHYD01000080
VERSION     NZ_JHYD01000080
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:33:25
            Original ID  :: NZ_JHYD01000080.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1880
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     aSDomain        1..1134
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg68_1_AMP-binding.1"
                     /evalue="5.30E-108"
                     /label="ctg68_1_AMP-binding.1"
                     /locus_tag="ctg68_1"
                     /protein_end="378"
                     /protein_start="0"
                     /score="353.0"
                     /specificity="consensus: leu"
                     /tool="antismash"
                     /translation="PDNIAVVFEEEKMTYRELNEKANQLAYKLRKLGVEPDDKVAILTQ
                     RSIEMVIGIYGIIKAGGAYVPIDPDCPEERIRYMLEDCQPKAVLLDKAELPVETDIPLI
                     NLFDQTIYTGRAQNPEHVNTPNDLIYIIYTSGTTGKPKGVMIEHGSVINLREYFVKTQG
                     LSETDIILQLSSITFDPSIAEMSMSLLIGGALCLISKECQQDTKLFKEYIMRKHITTIL
                     ITPLHLAQVDVCGLKTLITGGSEIDRELMLKNVHNCKVYFNDYGPTEATVCSTRWKYIN
                     GEMSLPERVPIGKPIANTQIYILNGNDLSGIGMPGELCIAGVGLARGYLNLPELTAEKF
                     VRNPFGKGRMYRTGDLARWLPDGNIEYLGRIDEQVKIR"
     PFAM_domain     1..1134
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg68_1_0001"
                     /evalue="6.00E-95"
                     /label="ctg68_1"
                     /locus_tag="ctg68_1"
                     /protein_end="378"
                     /protein_start="0"
                     /score="318.4"
                     /tool="antismash"
                     /translation="PDNIAVVFEEEKMTYRELNEKANQLAYKLRKLGVEPDDKVAILTQ
                     RSIEMVIGIYGIIKAGGAYVPIDPDCPEERIRYMLEDCQPKAVLLDKAELPVETDIPLI
                     NLFDQTIYTGRAQNPEHVNTPNDLIYIIYTSGTTGKPKGVMIEHGSVINLREYFVKTQG
                     LSETDIILQLSSITFDPSIAEMSMSLLIGGALCLISKECQQDTKLFKEYIMRKHITTIL
                     ITPLHLAQVDVCGLKTLITGGSEIDRELMLKNVHNCKVYFNDYGPTEATVCSTRWKYIN
                     GEMSLPERVPIGKPIANTQIYILNGNDLSGIGMPGELCIAGVGLARGYLNLPELTAEKF
                     VRNPFGKGRMYRTGDLARWLPDGNIEYLGRIDEQVKIR"
     aSModule        1..1647
                     /complete
                     /domains="nrpspksdomains_ctg68_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg68_1_PCP.1"
                     /locus_tags="ctg68_1"
                     /monomer_pairings="leu -> leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS             1..1878
                     /NRPS_PKS="Domain: AMP-binding (0-378). E-value: 5.3e-108.
                     Score: 353.0. Matches aSDomain:
                     nrpspksdomains_ctg68_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (482-549). E-value: 3e-24. Score:
                     76.9. Matches aSDomain: nrpspksdomains_ctg68_1_PCP.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     312.6; E-value: 6.6e-95)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg68_1"
                     /sec_met_domain="Condensation (E-value: 1.3e-19, bitscore:
                     56.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-117, bitscore:
                     378.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-16, bitscore:
                     46.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PDNIAVVFEEEKMTYRELNEKANQLAYKLRKLGVEPDDKVAILTQ
                     RSIEMVIGIYGIIKAGGAYVPIDPDCPEERIRYMLEDCQPKAVLLDKAELPVETDIPLI
                     NLFDQTIYTGRAQNPEHVNTPNDLIYIIYTSGTTGKPKGVMIEHGSVINLREYFVKTQG
                     LSETDIILQLSSITFDPSIAEMSMSLLIGGALCLISKECQQDTKLFKEYIMRKHITTIL
                     ITPLHLAQVDVCGLKTLITGGSEIDRELMLKNVHNCKVYFNDYGPTEATVCSTRWKYIN
                     GEMSLPERVPIGKPIANTQIYILNGNDLSGIGMPGELCIAGVGLARGYLNLPELTAEKF
                     VRNPFGKGRMYRTGDLARWLPDGNIEYLGRIDEQVKIRGFRIELGEIESVLKKQTGVKD
                     AAVAAKEKSGDKSICAYIVSDQEIRAGDIKDSLRKELPEYMVPAYILQIENIPVTRNGK
                     LDKRALPEPEAASGREYIAPRNKTEETIAGIFEEILGVTPVGIEDSFFEMGGHSLRATR
                     VVNQIEAKTGVRLALKTIFTAPTVKLLAKEVEGAKAKEYIPIPQAEEKEIYPMSSAQKR
                     LYLINQMDDEGITYNMPAGLEIQGRFDFDRMKSALEQLTARHEALRTSFH"
     protocluster    1..1880
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:1878](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..1878
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..1880
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1880
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     aSDomain        40..1206
                     /aSDomain="TIGR01733"
                     /aSTool="tigrfam"
                     /description="AA-adenyl-dom: amino acid adenylation domain"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg68_1_0001"
                     /evalue="7.90E-128"
                     /identifier="TIGR01733"
                     /label="ctg68_1"
                     /locus_tag="ctg68_1"
                     /protein_end="402"
                     /protein_start="13"
                     /score="424.7"
                     /tool="antismash"
                     /translation="TYRELNEKANQLAYKLRKLGVEPDDKVAILTQRSIEMVIGIYGII
                     KAGGAYVPIDPDCPEERIRYMLEDCQPKAVLLDKAELPVETDIPLINLFDQTIYTGRAQ
                     NPEHVNTPNDLIYIIYTSGTTGKPKGVMIEHGSVINLREYFVKTQGLSETDIILQLSSI
                     TFDPSIAEMSMSLLIGGALCLISKECQQDTKLFKEYIMRKHITTILITPLHLAQVDVCG
                     LKTLITGGSEIDRELMLKNVHNCKVYFNDYGPTEATVCSTRWKYINGEMSLPERVPIGK
                     PIANTQIYILNGNDLSGIGMPGELCIAGVGLARGYLNLPELTAEKFVRNPFGKGRMYRT
                     GDLARWLPDGNIEYLGRIDEQVKIRGFRIELGEIESVLKKQTGVKDAAV"
     CDS_motif       166..204
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0001"
                     /evalue="2.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg68_1"
                     /protein_end="68"
                     /protein_start="55"
                     /score="17.3"
                     /tool="antismash"
                     /translation="GIIKAGGAYVPID"
     CDS_motif       388..444
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0002"
                     /evalue="6.00E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg68_1"
                     /protein_end="148"
                     /protein_start="129"
                     /score="34.1"
                     /tool="antismash"
                     /translation="YIIYTSGTTGKPKGVMIEH"
     CDS_motif       793..822
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0003"
                     /evalue="2.70E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg68_1"
                     /protein_end="274"
                     /protein_start="264"
                     /score="10.4"
                     /tool="antismash"
                     /translation="YGPTEATVCS"
     CDS_motif       934..1020
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0004"
                     /evalue="8.20E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg68_1"
                     /protein_end="340"
                     /protein_start="311"
                     /score="53.3"
                     /tool="antismash"
                     /translation="IGMPGELCIAGVGLARGYLNLPELTAEKF"
     CDS_motif       1099..1164
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0005"
                     /evalue="8.50E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg68_1"
                     /protein_end="388"
                     /protein_start="366"
                     /score="40.1"
                     /tool="antismash"
                     /translation="YLGRIDEQVKIRGFRIELGEIE"
     PFAM_domain     1156..1374
                     /aSDomain="AMP-binding_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13193.8"
                     /description="AMP-binding enzyme C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg68_1_0002"
                     /evalue="2.20E-11"
                     /label="ctg68_1"
                     /locus_tag="ctg68_1"
                     /protein_end="458"
                     /protein_start="385"
                     /score="44.6"
                     /tool="antismash"
                     /translation="EIESVLKKQTGVKDAAVAAKEKSGDKSICAYIVSDQEIRAGDIKD
                     SLRKELPEYMVPAYILQIENIPVTRNGK"
     aSDomain        1447..1647
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg68_1_PCP.1"
                     /evalue="3.00E-24"
                     /label="ctg68_1_PCP.1"
                     /locus_tag="ctg68_1"
                     /protein_end="549"
                     /protein_start="482"
                     /score="76.9"
                     /tool="antismash"
                     /translation="EETIAGIFEEILGVTPVGIEDSFFEMGGHSLRATRVVNQIEAKTG
                     VRLALKTIFTAPTVKLLAKEVE"
     PFAM_domain     1453..1638
                     /aSDomain="PP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00550.27"
                     /description="Phosphopantetheine attachment site"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg68_1_0003"
                     /evalue="1.60E-11"
                     /label="ctg68_1"
                     /locus_tag="ctg68_1"
                     /protein_end="546"
                     /protein_start="484"
                     /score="44.4"
                     /tool="antismash"
                     /translation="TIAGIFEEILGVTPVGIEDSFFEMGGHSLRATRVVNQIEAKTGVR
                     LALKTIFTAPTVKLLAK"
     PFAM_domain     1693..1875
                     /aSDomain="Condensation"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00668.22"
                     /description="Condensation domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg68_1_0004"
                     /evalue="2.70E-15"
                     /label="ctg68_1"
                     /locus_tag="ctg68_1"
                     /protein_end="625"
                     /protein_start="564"
                     /score="56.1"
                     /tool="antismash"
                     /translation="KEIYPMSSAQKRLYLINQMDDEGITYNMPAGLEIQGRFDFDRMKS
                     ALEQLTARHEALRTSF"
     CDS_motif       1765..1878
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg68_1_0006"
                     /evalue="8.00E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg68_1"
                     /protein_end="626"
                     /protein_start="588"
                     /score="50.4"
                     /tool="antismash"
                     /translation="TYNMPAGLEIQGRFDFDRMKSALEQLTARHEALRTSFH"
ORIGIN
        1 ccagacaata ttgcggtcgt atttgaagaa gaaaaaatga cctatagaga gcttaatgaa
       61 aaagcaaacc agctggcata caagcttcgg aagcttggtg tggagccgga cgacaaggtt
      121 gctatcctga ctcaaagaag catcgagatg gttattggta tatatggtat catcaaagca
      181 ggcggagcct atgtgccgat agatccggac tgtccggaag agcgtataag atatatgctg
      241 gaggactgtc agcctaaagc agtactgctg gataaagcgg aactgcctgt agaaacggat
      301 attccgctca tcaacctctt tgatcagacg atttataccg gcagggctca aaatccggaa
      361 catgtgaata caccaaatga ccttatatat atcatttata catcaggaac gacaggtaaa
      421 ccaaaggggg ttatgattga acacggtagc gtaatcaatc tgcgggaata tttcgttaaa
      481 acacaaggat taagtgagac agatatcatc ttacaactct ccagtattac ctttgatcct
      541 tcgatagctg aaatgtctat gagtttgttg attgggggtg cattatgctt aatttcaaaa
      601 gaatgtcaac aagatacaaa gctatttaaa gaatatatta tgagaaaaca tattacaaca
      661 atactgatca ctcctttgca ccttgcacaa gtagacgtgt gcggactaaa aacacttatc
      721 acgggtgggt cagaaattga tcgggaactt atgctaaaga atgtccacaa ctgtaaagtg
      781 tattttaatg attatggtcc aacggaggca accgtttgtt caacaagatg gaaatatata
      841 aacggagaga tgtcgttgcc ggagcgagtg ccgattggga aaccgattgc aaacacgcaa
      901 atatatattc ttaacggtaa cgacctttcc ggtatcggaa tgccgggcga attgtgcatt
      961 gcaggagtag gcctggcccg gggatacctg aacctgcctg aattgacagc agagaaattt
     1021 gtcagaaatc cattcggtaa aggtagaatg taccgcaccg gggatctggc aagatggctg
     1081 ccggacggga atatcgagta tttgggcagg atcgacgagc aggtaaaaat ccgcggcttc
     1141 cgtattgagt tgggcgaaat agaaagtgtc ttgaaaaagc aaaccggtgt caaggatgcg
     1201 gcagtggcgg ccaaggagaa aagcggggac aagagcatct gtgcctatat agtatcggat
     1261 caggaaatcc gggcaggtga catcaaagac agccttagaa aagaactgcc ggaatatatg
     1321 gtacccgcat atattcttca gattgaaaac attccggtta cgaggaacgg aaagcttgac
     1381 aaacgtgccc tgccggaacc ggaagcagca agcgggcggg aatatattgc accccgaaac
     1441 aagacggaag aaaccattgc aggcatcttt gaagaaatat tgggcgttac cccggtggga
     1501 attgaagaca gcttctttga aatgggaggt cattccctgc gtgctacccg ggttgtcaac
     1561 caaatcgaag ccaagactgg tgtccgcctg gcattaaaga ccatatttac tgctcccacc
     1621 gtcaagcttc ttgccaaaga agtggaagga gcaaaagcaa aggaatatat tccaatccca
     1681 caggcggagg aaaaagaaat ctaccccatg tcgtcggctc aaaagagatt gtacttgatc
     1741 aaccagatgg acgatgaggg catcacctac aatatgccgg cggggctgga aatccaggga
     1801 agatttgatt ttgatcgtat gaagtccgca ttggagcaac tgaccgcacg ccacgaggct
     1861 ttacgcacta gctttcatag
//
