LOCUS       c00067_NZ_JHYD..        2215 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JHYD01000079.1 Ruminiclostridium cellobioparum DSM 1351 = ATCC
            15832 T344DRAFT_scaffold00067.67_C, whole genome shotgun sequence.
ACCESSION   NZ_JHYD01000079
VERSION     NZ_JHYD01000079
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:33:25
            Original ID  :: NZ_JHYD01000079.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2215
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS_motif       complement(1..42)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0007"
                     /evalue="9.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg67_1"
                     /protein_end="738"
                     /protein_start="724"
                     /score="18.7"
                     /tool="antismash"
                     /translation="LGVLKAGGAYLPID"
     PFAM_domain     complement(1..228)
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg67_1_0004"
                     /evalue="2.00E-20"
                     /label="ctg67_1"
                     /locus_tag="ctg67_1"
                     /protein_end="738"
                     /protein_start="662"
                     /score="72.9"
                     /tool="antismash"
                     /translation="FEEQMERTPDNIAIEAGGRCLTYRELNEKANRLARVLREKGVKQD
                     SIVAIMADQSVEFVVAVLGVLKAGGAYLPID"
     CDS             complement(1..2214)
                     /NRPS_PKS="Domain: PCP (127-195). E-value: 1.4e-24. Score:
                     77.9. Matches aSDomain: nrpspksdomains_ctg67_1_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (210-496). E-value:
                     2.5e-98. Score: 320.6. Matches aSDomain:
                     nrpspksdomains_ctg67_1_Condensation_LCL.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     335.1; E-value: 1.3e-101)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg67_1"
                     /sec_met_domain="Condensation (E-value: 2.1e-90, bitscore:
                     288.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1e-21, bitscore:
                     63.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.4e-15, bitscore:
                     43.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RLMDGNIEFVGRGDSQVKIRGYRIEPGEIENVLLKEESVKEAIVI
                     VKEDIKGDKMLCAYVVSDAQLEFTEIRRVLQKELPDYMIPAFIMHVDSIPLTHSGKVDR
                     KALPELIESLSRETEYEGPTNEIEQELMEIWTEVLGIKRMGINDNFFMMGGHSLKLMSL
                     VSNIHKRMNIDVPLKELFIRPTIRGLAEYLEKADVSIYKTIEPVEEKEYYPVSSAQKRM
                     YILSEFENVWTSYNTQLSVVVKGKLDVVRFEKAIKALVRRHESFRTSFGVVGDEIVQKV
                     NQDVDFQIEYMEASEEEAGNIVEAFVRPFDLGSAPLLRVGLIKIKESEYILICDMHHII
                     TDGVSHGILLNEFSALYEGRTLPELRLQYKDFAHWQNTFFKSGAIDKQREYWKEQFKDE
                     IPVLNMPTDYLRPKIQSFEGSFISFALDSNLTSKLKELTVETGTTMHMVLLTVLNVLLY
                     KYTGQEDVVIGTGIVGRPHADLENIVGMFVNMLAMRNHPQGSKTFKEFLKEVRESSLKA
                     YENQDYQFEMLVDTVNTRRDPERNPIYDVMFTAQNIDVPEIALEDVEFRPYHFGQSKTH
                     ADLNILAYIREDVIRFSLEYCTKLFRKETVERLARHFTNAAEEVVKNPDALLCEIKVMD
                     KDEEKDTLYSFNDTATQYPVGKTVCRLFEEQMERTPDNIAIEAGGRCLTYRELNEKANR
                     LARVLREKGVKQDSIVAIMADQSVEFVVAVLGVLKAGGAYLPID"
     protocluster    1..2215
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:2214](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(1..2214)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..2215
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..2215
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     PFAM_domain     complement(292..1584)
                     /aSDomain="Condensation"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00668.22"
                     /description="Condensation domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg67_1_0003"
                     /evalue="2.20E-121"
                     /label="ctg67_1"
                     /locus_tag="ctg67_1"
                     /protein_end="641"
                     /protein_start="210"
                     /score="405.9"
                     /tool="antismash"
                     /translation="EYYPVSSAQKRMYILSEFENVWTSYNTQLSVVVKGKLDVVRFEKA
                     IKALVRRHESFRTSFGVVGDEIVQKVNQDVDFQIEYMEASEEEAGNIVEAFVRPFDLGS
                     APLLRVGLIKIKESEYILICDMHHIITDGVSHGILLNEFSALYEGRTLPELRLQYKDFA
                     HWQNTFFKSGAIDKQREYWKEQFKDEIPVLNMPTDYLRPKIQSFEGSFISFALDSNLTS
                     KLKELTVETGTTMHMVLLTVLNVLLYKYTGQEDVVIGTGIVGRPHADLENIVGMFVNML
                     AMRNHPQGSKTFKEFLKEVRESSLKAYENQDYQFEMLVDTVNTRRDPERNPIYDVMFTA
                     QNIDVPEIALEDVEFRPYHFGQSKTHADLNILAYIREDVIRFSLEYCTKLFRKETVERL
                     ARHFTNAAEEVVKNPDALLCEIKVMDKDEEKD"
     CDS_motif       complement(568..684)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0006"
                     /evalue="1.90E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="ctg67_1"
                     /protein_end="549"
                     /protein_start="510"
                     /score="52.4"
                     /tool="antismash"
                     /translation="RESSLKAYENQDYQFEMLVDTVNTRRDPERNPIYDVMFT"
     aSDomain        complement(727..1584)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg67_1_Condensation_LCL.1"
                     /domain_subtype="Condensation_LCL"
                     /evalue="2.50E-98"
                     /label="ctg67_1_Condensation_LCL.1"
                     /locus_tag="ctg67_1"
                     /protein_end="496"
                     /protein_start="210"
                     /score="320.6"
                     /tool="antismash"
                     /translation="EYYPVSSAQKRMYILSEFENVWTSYNTQLSVVVKGKLDVVRFEKA
                     IKALVRRHESFRTSFGVVGDEIVQKVNQDVDFQIEYMEASEEEAGNIVEAFVRPFDLGS
                     APLLRVGLIKIKESEYILICDMHHIITDGVSHGILLNEFSALYEGRTLPELRLQYKDFA
                     HWQNTFFKSGAIDKQREYWKEQFKDEIPVLNMPTDYLRPKIQSFEGSFISFALDSNLTS
                     KLKELTVETGTTMHMVLLTVLNVLLYKYTGQEDVVIGTGIVGRPHADLENIVGMFVNML
                     AMRNH"
     CDS_motif       complement(733..822)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0005"
                     /evalue="1.00E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="ctg67_1"
                     /protein_end="494"
                     /protein_start="464"
                     /score="43.6"
                     /tool="antismash"
                     /translation="DVVIGTGIVGRPHADLENIVGMFVNMLAMR"
     CDS_motif       complement(1081..1113)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0004"
                     /evalue="7.60E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="ctg67_1"
                     /protein_end="378"
                     /protein_start="367"
                     /score="15.3"
                     /tool="antismash"
                     /translation="QYKDFAHWQNT"
     CDS_motif       complement(1144..1209)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0003"
                     /evalue="9.10E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="ctg67_1"
                     /protein_end="357"
                     /protein_start="335"
                     /score="31.0"
                     /tool="antismash"
                     /translation="MHHIITDGVSHGILLNEFSALY"
     CDS_motif       complement(1405..1518)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0002"
                     /evalue="6.20E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg67_1"
                     /protein_end="270"
                     /protein_start="232"
                     /score="50.8"
                     /tool="antismash"
                     /translation="TSYNTQLSVVVKGKLDVVRFEKAIKALVRRHESFRTSF"
     aSDomain        complement(1630..1833)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg67_1_PCP.1"
                     /evalue="1.40E-24"
                     /label="ctg67_1_PCP.1"
                     /locus_tag="ctg67_1"
                     /protein_end="195"
                     /protein_start="127"
                     /score="77.9"
                     /tool="antismash"
                     /translation="EQELMEIWTEVLGIKRMGINDNFFMMGGHSLKLMSLVSNIHKRMN
                     IDVPLKELFIRPTIRGLAEYLEK"
     PFAM_domain     complement(1636..1830)
                     /aSDomain="PP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00550.27"
                     /description="Phosphopantetheine attachment site"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg67_1_0002"
                     /evalue="7.20E-12"
                     /label="ctg67_1"
                     /locus_tag="ctg67_1"
                     /protein_end="193"
                     /protein_start="128"
                     /score="45.5"
                     /tool="antismash"
                     /translation="QELMEIWTEVLGIKRMGINDNFFMMGGHSLKLMSLVSNIHKRMNI
                     DVPLKELFIRPTIRGLAEYL"
     PFAM_domain     complement(1912..2133)
                     /aSDomain="AMP-binding_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13193.8"
                     /description="AMP-binding enzyme C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg67_1_0001"
                     /evalue="1.10E-11"
                     /label="ctg67_1"
                     /locus_tag="ctg67_1"
                     /protein_end="101"
                     /protein_start="27"
                     /score="45.7"
                     /tool="antismash"
                     /translation="EIENVLLKEESVKEAIVIVKEDIKGDKMLCAYVVSDAQLEFTEIR
                     RVLQKELPDYMIPAFIMHVDSIPLTHSGK"
     CDS_motif       complement(2125..2190)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg67_1_0001"
                     /evalue="3.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg67_1"
                     /protein_end="30"
                     /protein_start="8"
                     /score="38.2"
                     /tool="antismash"
                     /translation="FVGRGDSQVKIRGYRIEPGEIE"
ORIGIN
        1 atctataggc agatatgccc ctcccgcttt aagaacgccc agcactgcta caacaaattc
       61 tacagactgg tcggccatga ttgccactat ggagtcttgc tttacacctt tttcccttaa
      121 tacccttgca agccggtttg ctttttcatt cagctccctg taagtcagac acctgcctcc
      181 ggcttcaatg gcaatattgt ccggagttct ttccatctgc tcttcaaaca accggcaaac
      241 agtttttccc actggatatt gggtcgcagt gtcgttgaag ctatatagag tatccttttc
      301 ttcatcctta tccatcactt ttatttcaca aagcagtgca tctggatttt tcaccacctc
      361 ttccgctgcg tttgtaaaat gccgtgccaa cctctccact gtttcctttc ggaataactt
      421 tgtgcagtac tccaacgaaa atctaattac atcttcacga atatatgcca gaatgttcaa
      481 atcagcatgg gtcttgcttt gcccaaagtg atatggcctg aactctacat cctcaagtgc
      541 tatttcaggg acatcaatat tctgagctgt aaacatcaca tcatatattg gattcctttc
      601 gggatctctt ctggtattta cagtatccac tagcatctca aactgatagt cctgattctc
      661 gtaggctttt aaagagcttt cccttacttc tttaagaaac tccttaaagg ttttacttcc
      721 ttggggatga tttctcattg caagcatgtt tacaaacatt ccaactatgt tttccaggtc
      781 tgcatggggt cttcccacta tccccgtccc tattaccaca tcttcctgtc ctgtatattt
      841 atagagtagt acgttcaata ccgtcaggag taccatatgc attgttgtac ctgtctctac
      901 agtgagttcc tttaattttg aagtcaggtt actatctaat gcaaaagaaa taaagcttcc
      961 ttcaaagctc tgtattttgg gtcttagata atctgtaggc atattaagga ccggtatctc
     1021 gtctttaaat tgctccttcc agtactccct ctgcttatca attgctcccg atttgaagaa
     1081 agtgttttgc caatgtgcga aatccttata ttgcagcctc aactccggaa gagtccttcc
     1141 ctcataaagt gctgaaaatt cattaagaag tataccgtga gagactccat cagtgatgat
     1201 atggtgcata tcgcatatta agatgtactc agactctttt atttttatca atcctaccct
     1261 taatagggga gcactgccca aatcaaaggg tcttacaaat gcttccacga tgttgcccgc
     1321 ttcttcttca ctcgcctcca tatactctat ctgaaaatct acatcctggt tcactttctg
     1381 tactatttcg tccccaacta ccccgaagga ggtccggaat gattcatgtc ttcttacaag
     1441 agctttaatg gctttttcaa atcttactac atctaactta cctttcacca ccacagacag
     1501 ctgtgtattg tagctagtcc aaacgttctc aaattcgcta agtatataca ttctcttttg
     1561 ggctgaagat acgggataat actccttctc ctcaaccggc tctatggtct tgtaaatact
     1621 cacatcggct ttttctaaat attcagccaa tcctcttatt gtggggcgta taaagagttc
     1681 cttcagcggt acatcaatat tcattctctt gtgtatattt gagacaaggc tcataagctt
     1741 caaagaatgt ccgcccatca taaagaagtt gtcattaatc cccatccgct ttatgccaag
     1801 aacctctgtc catatttcca tcagttcctg ttctatctca ttggtaggcc cttcatactc
     1861 ggtttctcgg gacaggctct ctatcagctc aggcagagct tttctgtcca ccttcccgct
     1921 gtgggtcaga gggatgctat ccacatgcat tataaatgca ggtatcatgt agtcagggag
     1981 ctccttttgt aatacccttc ttatttcagt aaactccagt tgtgcatcac ttaccacata
     2041 tgcacacagc atcttatccc cttttatatc ttcctttact atgactattg cctcttttac
     2101 cgattcttcc tttaataata cattctctat ttcacccggc tctatcctgt aaccccttat
     2161 ctttacctga ctgtcacctc tgcccacgaa ctctatgttt ccatccatga gccgc
//
