LOCUS       c00049_NZ_LNAM..        2910 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_LNAM01000049.1 Acetivibrio ethanolgignens strain ACET-33324
            ACET-33324.Scaf142, whole genome shotgun sequence.
ACCESSION   NZ_LNAM01000049
VERSION     NZ_LNAM01000049
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:56:40
            Original ID  :: NZ_LNAM01000049.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2910
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2910
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2245:2869](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2246..2869
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     cand_cluster    1..2910
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..2910
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     CDS             complement(27..194)
                     /locus_tag="ctg49_1"
                     /transl_table=11
                     /translation="MILKSSIIVDLKINSLEDLYKLKPFTENSSLNINKSQIARELGVD
                     TALRNKNFSH"
     CDS             450..1733
                     /locus_tag="ctg49_2"
                     /transl_table=11
                     /translation="MLHLVNKYMDEYLADHNNLSAKAKAEKYAKITAKTIISSGGADSA
                     SYGQNAFFYDAAEGVLTAVILLIAEFCPKEKRHIISVFKLIQDLLAPSKVKGRNQFQLL
                     MAKLPDTHKAKWFAGAALNTAEQSMQSVLSTALSRLNSFLDSELEQILCFDTAIDAEMF
                     CKQKTAVFLVMPEEDNTKYFIISLILQQLYREILVVADENGGKLDNRVVFYWDEVGTIP
                     KIESAEMMFSASRSRRVSIVPMIQSFAQLNKNYGKEGAEIIIDNCQDTIFGGFAPNSES
                     AEVLSKSLGNKTVMSGSISRGKNDPSQSLQMIQRPLMTPDELKSLPKGNFIVSKTGSHP
                     MRTKLKLFLKWGITFEEPYEIEEKSARKVAYADKQEIEEEIIRRYMCCVDEEPESTEQG
                     TASRSGGMLQSQMNETVAKVRQALRTED"
     PFAM_domain     537..1490
                     /aSDomain="T4SS-DNA_transf"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02534.16"
                     /description="Type IV secretory system Conjugative DNA
                     transfer"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_2_0001"
                     /evalue="2.00E-35"
                     /label="ctg49_2"
                     /locus_tag="ctg49_2"
                     /protein_end="347"
                     /protein_start="29"
                     /score="122.6"
                     /tool="antismash"
                     /translation="KITAKTIISSGGADSASYGQNAFFYDAAEGVLTAVILLIAEFCPK
                     EKRHIISVFKLIQDLLAPSKVKGRNQFQLLMAKLPDTHKAKWFAGAALNTAEQSMQSVL
                     STALSRLNSFLDSELEQILCFDTAIDAEMFCKQKTAVFLVMPEEDNTKYFIISLILQQL
                     YREILVVADENGGKLDNRVVFYWDEVGTIPKIESAEMMFSASRSRRVSIVPMIQSFAQL
                     NKNYGKEGAEIIIDNCQDTIFGGFAPNSESAEVLSKSLGNKTVMSGSISRGKNDPSQSL
                     QMIQRPLMTPDELKSLPKGNFIVSKTGSHPMRTKLKL"
     CDS             1742..1996
                     /locus_tag="ctg49_3"
                     /transl_table=11
                     /translation="LSKKEIYEAELPHRAVAVYLYLKDRANKEGICYPAIGTIARELHL
                     SVSTIKRAISDLEENGFIRKKQRWRDNGGRSSLLFEIIK"
     PFAM_domain     1769..1930
                     /aSDomain="HTH_36"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13730.8"
                     /description="Helix-turn-helix domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_3_0002"
                     /evalue="9.40E-14"
                     /label="ctg49_3"
                     /locus_tag="ctg49_3"
                     /protein_end="63"
                     /protein_start="9"
                     /score="51.2"
                     /tool="antismash"
                     /translation="ELPHRAVAVYLYLKDRANKEGICYPAIGTIARELHLSVSTIKRAI
                     SDLEENGFI"
     CDS             2246..2869
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg49_4"
                     /sec_met_domain="AgrB (E-value: 1.3e-39, bitscore: 123.6,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNKIVETLINKQSQNEIITNEDEQIYRYGYILLCEVVLNLVIAL
                     IIGIVFLKTKEIMFFLCVYIPLRSFCGGWHANKIWKCTIISNAILLLQVYCIENIVNLI
                     SSERMLIICFFNMICVFFLAPIETEKKKISHEERLIYRKKIKVMLVLHLITILATILWD
                     NKQLEFSIMFAYITQSVMLLLEIAKISLSKRVIFRKNQEYSFEE"
     PFAM_domain     2264..2791
                     /aSDomain="AgrB"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04647.17"
                     /description="Accessory gene regulator B"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_4_0003"
                     /evalue="6.80E-36"
                     /label="ctg49_4"
                     /locus_tag="ctg49_4"
                     /protein_end="182"
                     /protein_start="6"
                     /score="123.4"
                     /tool="antismash"
                     /translation="ETLINKQSQNEIITNEDEQIYRYGYILLCEVVLNLVIALIIGIVF
                     LKTKEIMFFLCVYIPLRSFCGGWHANKIWKCTIISNAILLLQVYCIENIVNLISSERML
                     IICFFNMICVFFLAPIETEKKKISHEERLIYRKKIKVMLVLHLITILATILWDNKQLEF
                     SIMFAYITQSVML"
ORIGIN
        1 tgtaaatggc tataaatttt ttaaaattaa tgcgaaaagt ttttattcct caaagctgtg
       61 tcaacaccaa gctctcttgc aatctggctt ttattgatgt ttagtgaaga attttctgta
      121 aacggtttca atttgtataa gtcctccaat gaattaatct ttaaatctac aataatgctg
      181 ctttttaaaa tcatactgtt tacctccatg gtaacagtat agcatttgta catttttatt
      241 ttggattttt tgnnnnnnnn nntatagcat ttgtacattt ttattttgga ttttttgtac
      301 atatatattg taccatttat aggagttttc tgactacgga taccaagggt gacctttacc
      361 gaaactatgc aggcatcgcc aagaaatact atgggtacca caccgcagtc attgacttaa
      421 gaaacccaac tagaagtgat ggcgataata tgctccattt ggtaaacaag tacatggatg
      481 agtatctggc agatcataac aacttaagtg ccaaggcaaa ggcagaaaag tatgcaaaga
      541 ttactgccaa gacaatcata tcctctgggg gagccgacag tgccagctac ggacaaaatg
      601 cattcttcta tgatgccgca gaaggtgtgc tgaccgcagt catcctgtta atagcagaat
      661 tctgtcccaa ggagaaaaga cacatcatta gtgtatttaa actgattcag gatttactgg
      721 cgccatccaa ggtaaaagga agaaatcagt ttcagttgct tatggcaaag cttccggata
      781 cccacaaagc caaatggttt gccggggcag ctttgaatac cgcagagcag tccatgcagt
      841 ctgttctttc cacagcactc tccagactaa actctttcct cgattctgaa ctggagcaga
      901 tactgtgctt tgataccgct atcgatgctg aaatgttttg taagcagaag acagcggtat
      961 ttttggttat gccagaagag gacaatacca agtattttat tatctctctg attttgcagc
     1021 agctctacag ggaaatcctt gtggtggcag atgagaacgg agggaagctg gacaaccgtg
     1081 ttgttttcta ttgggatgag gtcggaacca taccgaagat tgagagtgcg gagatgatgt
     1141 tctcggcatc ccgttccaga agggtaagta tcgtacctat gatacagtcc tttgcacagc
     1201 ttaataagaa ctacggaaag gaaggggcag agattatcat cgataactgt caggatacca
     1261 tcttcggtgg atttgcaccg aactcagaat ccgcagaggt actgtccaag agccttggaa
     1321 acaagacggt catgtccggc tccatcagca ggggaaagaa tgatcccagc cagtccctcc
     1381 agatgataca aagaccatta atgacaccgg atgagttaaa gtccctgcca aaaggaaact
     1441 ttattgtgtc aaaaacaggc agccatccca tgagaaccaa gctgaagctg ttcctaaagt
     1501 ggggtattac ctttgaggaa ccatatgaga ttgaggaaaa gtcagcgaga aaggtagcct
     1561 atgcagacaa gcaggagatt gaagaagaga ttatccgcag atatatgtgc tgtgtggatg
     1621 aggagccgga aagcacggaa caaggcactg catccagaag tggtggcatg ctgcagtccc
     1681 agatgaatga aacggtggca aaggtcagac aggctctcag aacagaggat taggtgatta
     1741 gttgagcaaa aaagaaatat atgaagcaga actgccccat agggctgtgg cggtatatct
     1801 gtacctaaag gatcgtgcaa ataaggaagg catctgctat ccggcaatcg gtaccattgc
     1861 cagggaactg catttgtctg tcagcaccat aaaacgtgcc atcagcgatt tggaggagaa
     1921 cggatttata cgcaagaaac agcgctggcg tgataatgga ggcagaagca gtctgctgtt
     1981 tgaaataata aagtaagcac aaaggggata ctgcgtaggt taggtgaact atggtatggt
     2041 tcaccatgac ctatgcaggg aacttttcac tttaaggatt aggacagaga aaagataaaa
     2101 tatatattac agaatcaaag tatagtgaag atataaatag gcttaaaaac gaaattggga
     2161 catagattgt caaatttagg acatttgcac aattcatgtt atgaattatg ttaaattgct
     2221 caaaaactaa agaggtagaa tctatatgac taataaaata gtagagacac taataaataa
     2281 acaatctcaa aacgaaataa taacaaatga agatgagcag atatatagat atggatatat
     2341 tttattgtgt gaagttgttt taaatttagt gatagcctta ataataggaa ttgtattttt
     2401 gaaaacaaag gagataatgt tttttttgtg cgtttatatt ccgctaagaa gtttttgtgg
     2461 aggatggcat gcaaataaaa tttggaaatg tacaataata tcaaatgcaa tattgttatt
     2521 gcaagtgtat tgtatagaaa atatagtcaa tctcatatca agcgaaagaa tgttgataat
     2581 atgttttttt aatatgattt gtgtattttt tttggcgcca atagaaacgg agaagaaaaa
     2641 aattagtcat gaagagaggc tgatttatag aaaaaaaata aaggtaatgt tagtgcttca
     2701 tctaataaca atattagcaa caatactttg ggataacaag caattggaat ttagtattat
     2761 gtttgcgtat ataacacaaa gtgttatgtt attattggaa atagcgaaaa tttcactttc
     2821 taaaagagtt atttttagaa aaaatcaaga gtacagcttt gaggaataaa aacttttcgc
     2881 attaatttta aaaaatttat agccatttac
//
