LOCUS       NZ_CP016839.1          10288 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP016839.1 Brochothrix thermosphacta strain TMW 2.1564
            chromosome, complete genome.
ACCESSION   NZ_CP016839
VERSION     NZ_CP016839.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 14:18:37
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 309613
            Orig. end    :: 319901
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[314613:314901](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5288
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193)"
                     /tool="antismash"
     CDS             complement(823..2040)
                     /gene_functions="transport (smcogs) SMCOG1169:sugar
                     transport protein (Score: 186.7; E-value: 1.2e-56)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_287"
                     /transl_table=11
                     /translation="MSKKKNEKQTISRNKLLKVAGFGWLFDAMDVGILSFVIAALAIEW
                     QLTPTEMGWIGSVNSIGMAVGAFVFGVFSDRVGRKNIFMITLIVFSVANGLSAFTTSLA
                     AFMVLRFIVGMGLGGELPVASTLVSESVAAHERGRVVVLLESFWAGGWLLAALISYFVI
                     PTYGWRVALLITAIPALYAIYLRIHLPDSPRFTAKKEQEKGSVWQNIKTVWSKQYKRRT
                     LMLWIVWFTVVFSYYGMFLWLPSVMTMKGFSLIKSFEYVLIMTLAQLPGYFTAAWLIER
                     AGRKFVLAAYLTGTAISALVFGNADTTMMLIISGMFLSFFNLGAWGALYAYSPEQYPTV
                     VRGTGTGMAAAVGRLGGVLGPLLVGMMLSNGSSINTIFSIFCGAILVGVLAVVFLGTET
                     KQLELD"
     PFAM_domain     complement(829..1437)
                     /aSDomain="Sugar_tr"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00083.26"
                     /description="Sugar (and other) transporter"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_287_0022"
                     /evalue="1.90E-10"
                     /label="ctg1_287"
                     /locus_tag="ctg1_287"
                     /protein_end="404"
                     /protein_start="201"
                     /score="40.3"
                     /tool="antismash"
                     /translation="EKGSVWQNIKTVWSKQYKRRTLMLWIVWFTVVFSYYGMFLWLPSV
                     MTMKGFSLIKSFEYVLIMTLAQLPGYFTAAWLIERAGRKFVLAAYLTGTAISALVFGNA
                     DTTMMLIISGMFLSFFNLGAWGALYAYSPEQYPTVVRGTGTGMAAAVGRLGGVLGPLLV
                     GMMLSNGSSINTIFSIFCGAILVGVLAVVFLGTETKQLEL"
     PFAM_domain     complement(1432..1896)
                     /aSDomain="Sugar_tr"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00083.26"
                     /description="Sugar (and other) transporter"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_287_0021"
                     /evalue="8.20E-33"
                     /label="ctg1_287"
                     /locus_tag="ctg1_287"
                     /protein_end="203"
                     /protein_start="48"
                     /score="114.0"
                     /tool="antismash"
                     /translation="PTEMGWIGSVNSIGMAVGAFVFGVFSDRVGRKNIFMITLIVFSVA
                     NGLSAFTTSLAAFMVLRFIVGMGLGGELPVASTLVSESVAAHERGRVVVLLESFWAGGW
                     LLAALISYFVIPTYGWRVALLITAIPALYAIYLRIHLPDSPRFTAKKEQEK"
     CDS             complement(2330..2905)
                     /locus_tag="ctg1_288"
                     /transl_table=11
                     /translation="MKHKMLLGTIIVAVIMLSACGFNITQSSDSGTKKIKTDELFAMDY
                     KVAKADVDKPFIFTVERYDNGVLTSTEEIKLANEERSGTLRVLSEKDAFANFWFTSGKG
                     KQERQEHIKLKDVDKLSGQMVSGQISKSAFLRDFNKEQRFGYVAFDENPDVQNTKITFS
                     TFLKNQQDKDFIKQYKTIFFFNVRLADK"
     CDS             complement(2918..3541)
                     /locus_tag="ctg1_289"
                     /transl_table=11
                     /translation="MSKKNILISTVVLFIAIGSLAYFMFILPPKTSYIKMHKLASTEES
                     SAHMMGFDYKLNKNDMTKKAIALTLEHYTKGEYIKSFEPVIITVSEANYFTISVSPVEK
                     NRVYLSVRNQYTTSSWEPSFVEHKHQMASMIDNVVTGKTSLSEKEVVIGYLNIVTSTQG
                     MLDSSVDQNRKLVKAADGKTKQSLAKFDELYVFKAKLIDDKINK"
     CDS             complement(3566..4195)
                     /locus_tag="ctg1_290"
                     /transl_table=11
                     /translation="VKRKTLLSLAILVLIIGGATSWLLLKAPKESYLKSNDLVGADEWK
                     GVLHLIGTDYRLNKTDAERTLVVSLTHYKNGKRMGKSEPLATGLAEKKGDFKISFLFDQ
                     NDVTHTDYQFSLVNDGTITNWPEKPVVLKEKDQFVTDGTLSEKTAIKNKPVVGYAYRQK
                     DNKRVLSRKDDAALIVKYNQPKPEIAALKAYDDLYLFTAEFKKAIK"
     CDS             4429..4635
                     /locus_tag="ctg1_291"
                     /transl_table=11
                     /translation="MRKKLRNMLIYIISFLIAFQIIKAASNTFFLYTAVDVTIGSEFIT
                     VLILFALTATFAETTILIIKKKS"
     CDS             5001..5288
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_292"
                     /sec_met_domain="Lactococcin_972 (E-value: 5.7e-14,
                     bitscore: 49.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LKKILLLSILTIGLLGSVATTALAYEVNVGGGTWNYGNGKIYSWS
                     NYYHKSKTHKSSVTVGNYTTSSDWISKNKWAKASAKKSLWSTSQFYWNTK"
     aSDomain        5013..5282
                     /aSDomain="TIGR01653"
                     /aSTool="tigrfam"
                     /description="lactococcin_972: bacteriocin, lactococcin 972
                     family"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_292_0002"
                     /evalue="8.40E-14"
                     /identifier="TIGR01653"
                     /label="ctg1_292"
                     /locus_tag="ctg1_292"
                     /protein_end="94"
                     /protein_start="4"
                     /score="49.6"
                     /tool="antismash"
                     /translation="LLLSILTIGLLGSVATTALAYEVNVGGGTWNYGNGKIYSWSNYYH
                     KSKTHKSSVTVGNYTTSSDWISKNKWAKASAKKSLWSTSQFYWNT"
     PFAM_domain     5088..5273
                     /aSDomain="Lactococcin_972"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF09683.12"
                     /description="Bacteriocin (Lactococcin_972)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_292_0023"
                     /evalue="6.90E-14"
                     /label="ctg1_292"
                     /locus_tag="ctg1_292"
                     /protein_end="91"
                     /protein_start="29"
                     /score="52.2"
                     /tool="antismash"
                     /translation="GGGTWNYGNGKIYSWSNYYHKSKTHKSSVTVGNYTTSSDWISKNK
                     WAKASAKKSLWSTSQFY"
     CDS             5515..7608
                     /locus_tag="ctg1_293"
                     /transl_table=11
                     /translation="MKKIWSLFFVIGLITTLFLATQLYKAKEDYDSIYLTKANTEAISV
                     TDQPSHLSVNALNKEINSFTKANKINLYRQFYNESVSSQYNKTVYVSIGDINGVSSLYD
                     LSKNDSEKLLTNGTLSTKNNEIKFFNPITNVQLTTLKNAENEGLTGYYFIQSDTPEKIK
                     KTKDFLLQKGLIFEDVKKISRTDLIDTIMSENMIVPLIISILIFLITFIFISFFEVMQR
                     FKEIAIYQTLGYNNRQIKVAFIHQKLRIIIYMILISICAIFAWCLWFNGGIQIVELFIF
                     STVFIVVYLLSVLFIYYLCLISMKFITINLMIKGKKPYHKLLIVQQTFKFLCLAILLIL
                     SIHTTNEIRQLNYQIAQLETFNTVKKFNTTHISITTASKDAEEVVEDKIYSLNTALDKK
                     GSFILSPSNFYLNPAEFGDNSDELPYVLVSLNYFKANPQLGIDYAQLKQSLNNKGDLVA
                     LFPDNYRLDKDVFKTNLTELLILSKKTPPHVLSKTYPHQKIPIYDYTFTKFSDTNIESP
                     IFVVFDSKKASPSTILSAYSNGLAFLNLGENDAFKKLTPLLEKYKLVDNIRNINNVKKT
                     VTQQIVLLQNELNVRIFTLLTLTTVLLITLFFITNLYIEYQQKMLAVKKCHGYNLLQRH
                     IFFLLTELLVLLAVLVSVSLMITLNSRTLIFIAAALMITFIITLFFVIVFEKNSINKVM
                     KGE"
     PFAM_domain     7300..7602
                     /aSDomain="DUF1430"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07242.13"
                     /description="Protein of unknown function (DUF1430)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_293_0024"
                     /evalue="1.20E-06"
                     /label="ctg1_293"
                     /locus_tag="ctg1_293"
                     /protein_end="696"
                     /protein_start="595"
                     /score="28.8"
                     /tool="antismash"
                     /translation="LTLTTVLLITLFFITNLYIEYQQKMLAVKKCHGYNLLQRHIFFLL
                     TELLVLLAVLVSVSLMITLNSRTLIFIAAALMITFIITLFFVIVFEKNSINKVMKG"
     CDS             7611..8219
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 170.9; E-value:
                     5.9e-52)"
                     /gene_kind="transport"
                     /locus_tag="ctg1_294"
                     /transl_table=11
                     /translation="MIRITNISKKFQGNSVVSNFSEHILENEMVAIMGPSGSGKTTLLN
                     MIAGIEECDSGTVTINNNKNISNIFKLRYDIALLFQNYGLIDQITVEDNLMIAMKFSQE
                     KNKNEAIKAVLIEFNLYDKRKQKVFTLSGGEQQRIALARIMLKPHTIILADEPTGSLDA
                     INKEFVMSQLIKFKKAGKTIVVVTHDQTISALCDRTITL"
     PFAM_domain     7662..8084
                     /aSDomain="ABC_tran"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00005.29"
                     /description="ABC transporter"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_294_0025"
                     /evalue="8.10E-32"
                     /label="ctg1_294"
                     /locus_tag="ctg1_294"
                     /protein_end="158"
                     /protein_start="17"
                     /score="110.7"
                     /tool="antismash"
                     /translation="SNFSEHILENEMVAIMGPSGSGKTTLLNMIAGIEECDSGTVTINN
                     NKNISNIFKLRYDIALLFQNYGLIDQITVEDNLMIAMKFSQEKNKNEAIKAVLIEFNLY
                     DKRKQKVFTLSGGEQQRIALARIMLKPHTIILADEPT"
     aSDomain        8233..8544
                     /aSDomain="TIGR01655"
                     /aSTool="tigrfam"
                     /description="yxeA_fam: conserved hypothetical protein"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_295_0003"
                     /evalue="1.40E-11"
                     /identifier="TIGR01655"
                     /label="ctg1_295"
                     /locus_tag="ctg1_295"
                     /protein_end="104"
                     /protein_start="0"
                     /score="43.0"
                     /tool="antismash"
                     /translation="MKKIFFLTIALVVLILIFSYSTKDIAAVNRFNPFVKETTSYAKVP
                     TGTQIYKGITLIDNKKNTVIVDFGGYDPEGVYVKVKHKRNFVKHVDYITAEKIPETIRK
                     "
     CDS             8233..8559
                     /locus_tag="ctg1_295"
                     /transl_table=11
                     /translation="MKKIFFLTIALVVLILIFSYSTKDIAAVNRFNPFVKETTSYAKVP
                     TGTQIYKGITLIDNKKNTVIVDFGGYDPEGVYVKVKHKRNFVKHVDYITAEKIPETIRK
                     DITH"
     CDS             complement(8666..9016)
                     /locus_tag="ctg1_296"
                     /transl_table=11
                     /translation="LENHRIYTTSFASVYPLYIKKAERKQRTKEEVDKIIFWLTGYDTT
                     SLNQQLEDAVDFKQFFAEAPQMNPKTDLIKGVVCGIRVEEIEQPLMQQIRYLDKLIDEL
                     AKGKKMDKILRN"
     PFAM_domain     complement(8672..9004)
                     /aSDomain="DUF2200"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF09966.11"
                     /description="Uncharacterized protein conserved in bacteria
                     (DUF2200)"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_296_0026"
                     /evalue="1.20E-53"
                     /label="ctg1_296"
                     /locus_tag="ctg1_296"
                     /protein_end="115"
                     /protein_start="4"
                     /score="180.0"
                     /tool="antismash"
                     /translation="RIYTTSFASVYPLYIKKAERKQRTKEEVDKIIFWLTGYDTTSLNQ
                     QLEDAVDFKQFFAEAPQMNPKTDLIKGVVCGIRVEEIEQPLMQQIRYLDKLIDELAKGK
                     KMDKILR"
     CDS             complement(9110..9739)
                     /locus_tag="ctg1_297"
                     /transl_table=11
                     /translation="MTIAKQTAKHLLEIEAVFLKPEEPFTWASGIKSPIYCDNRMTLGF
                     PNVRRAIAKGLAAEIKANYPDVDVIAGTATAGIPHAAFIAEELDLPMIYVRSEPKKHGK
                     GKQIEGPLAPGKKVVMIEDLISTAGSCLKAAKAVEEAGSEVIAIAAIFTYELAAAQRNL
                     EAANIPLLTLTNYSTLLEVAAEDGYVKEAELETLREWNKDPENWNK"
     aSDomain        complement(9206..9715)
                     /aSDomain="TIGR00336"
                     /aSTool="tigrfam"
                     /description="pyrE: orotate phosphoribosyltransferase"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg1_297_0004"
                     /evalue="7.20E-46"
                     /identifier="TIGR00336"
                     /label="ctg1_297"
                     /locus_tag="ctg1_297"
                     /protein_end="178"
                     /protein_start="8"
                     /score="154.1"
                     /tool="antismash"
                     /translation="KHLLEIEAVFLKPEEPFTWASGIKSPIYCDNRMTLGFPNVRRAIA
                     KGLAAEIKANYPDVDVIAGTATAGIPHAAFIAEELDLPMIYVRSEPKKHGKGKQIEGPL
                     APGKKVVMIEDLISTAGSCLKAAKAVEEAGSEVIAIAAIFTYELAAAQRNLEAANIPLL
                     TLTNYST"
     PFAM_domain     complement(9284..9586)
                     /aSDomain="Pribosyltran"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00156.29"
                     /description="Phosphoribosyl transferase domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg1_297_0027"
                     /evalue="1.50E-11"
                     /label="ctg1_297"
                     /locus_tag="ctg1_297"
                     /protein_end="152"
                     /protein_start="51"
                     /score="44.0"
                     /tool="antismash"
                     /translation="IAKGLAAEIKANYPDVDVIAGTATAGIPHAAFIAEELDLPMIYVR
                     SEPKKHGKGKQIEGPLAPGKKVVMIEDLISTAGSCLKAAKAVEEAGSEVIAIAAIF"
ORIGIN
        1 aattgttggg gaaggagtta aatttttcaa tttgataggg gttaattttt tcaatgcggt
       61 taaaggctct tccgttggta tgccagtcct tggatagcaa gttttcttgt agcaaaccag
      121 tttcaatgcc ttgtgctctc ccataacgtt taagaccgaa ccaattgctt gctcgattat
      181 ttggccaact aacagcttgt ttacctgttt taattgaaac aacttgaaat gtgatgtcac
      241 gaggatcttt actataaaaa ccccagcctt gcggaaacca ttgctgcgtt ttaatcatct
      301 ttccaaacgg tagctgactg attgggttct ttgcagttac tggcattaaa gcatacagat
      361 aaagaacact gtatagcagt atcaaactta caaataaaat aaattgcttg gtcattttaa
      421 caacctccca atataataat ataaaattaa caaggccttt tataaggtca acttaacatt
      481 tacagtttaa aaaagtcaat agatatgcga cattaatctg ttttttcagt ctttttgaag
      541 aagctaaata aacagtgtaa ttccactaaa cagcctttta ggggctgcta atcatctgaa
      601 aattgattat aaactgtttc atctctcata tcttcgtcga aaaactgttt taattcgttg
      661 attaaacaag cggcttgtca tcctatcatt ccgcctcaaa aaaaataaca tcctgacggc
      721 aacgtcaaaa tgttattctt caatatatta gtaccaaagc agtatcgctc ttttattcaa
      781 atgatggacc attattttag cgtcaccttt tgtgagtatc acttaatcca attcaagttg
      841 ttttgtttct gtgccgagga aaacaacagc taaaacaccc actagaatgg caccgcaaaa
      901 gatgctgaag attgtattta ttgaagagcc gttcgataac atcattccga ctaacaatgg
      961 acctaacacc cctcctaaac gtccgacagc tgctgccata cctgttccag tcccacgaac
     1021 gactgttggg tattgttctg gagaataagc atacaacgct ccccaagcac ctaggttaaa
     1081 gaatgataag aacattcccg aaataatcaa catcatcgtt gtatctgcat taccaaatac
     1141 caatgccgaa attgctgtcc cagtgaggta agctgctaat acaaactttc gtccagctcg
     1201 ttcaattaac catgcggctg taaaataacc tggcaattgt gcaagcgtca taatcaacac
     1261 gtattcaaag cttttaatta ggctaaaacc tttcattgtc atgactgacg gcagccataa
     1321 aaacatgcca taataagaaa agacaacggt gaaccagaca atccataaca tcaacgtacg
     1381 acgtttatat tgtttggacc aaaccgtttt gatattttgc caaacgctgc ctttttcctg
     1441 ttcttttttt gctgtgaaac gtggtgaatc tggtaagtga atgcgtaaat aaatcgcata
     1501 aagtgcagga attgccgtaa tcaacaatgc aactcgccaa ccgtaagttg gaataacaaa
     1561 gtaagaaata agtgcagcta ataaccaacc acctgcccaa aaactttcaa gtaaaacaac
     1621 cactcgacca cgttcgtgtg ctgcaacact ttccgatact aatgttgatg caactggtag
     1681 ctcaccacca agccccatcc caacaataaa gcgcaatacc ataaatgcag ctaaggatgt
     1741 tgtgaacgct gacaatccgt tcgctaccga aaacacgatc aatgtaatca taaagatatt
     1801 tttgcgtcct actctatctg agaaaacacc aaaaacaaaa gctccaaccg ccatcccaat
     1861 tgagttgaca ctaccaatcc aacccatctc tgtcggtgtt aactgccatt caattgcaag
     1921 tgccgcgata acaaaagata aaattccaac atccattgcg tcaaacaacc aaccaaaacc
     1981 agcaactttc aacaacttgt tacgtgaaat cgtttgtttc tcattcttct ttttagacat
     2041 aatatccccc ttaaagaaac attcccttaa tatttcctca agaccaaagt taaccaataa
     2101 ttctgactgt tatgcttcag tcttaataag ttcttagaaa aagatttaaa ctaatgtttt
     2161 gaaaatacat tgagttttaa attacatttt cgacacactc acagaaatac gtccttaaca
     2221 tttcttcttt gtccctttaa tatatacata agcttctaga aaaacaaggg ttctctcaaa
     2281 aaaacatctc ttctaacccc aaattaatga agttttaaga gatgtcctat tatttatctg
     2341 ctagacgtac attaaagaaa aatattgttt tatattgctt aataaagtct ttatcttgct
     2401 gattctttaa aaaggttgaa aatgttattt tagtattttg gacatctgga ttttcatcaa
     2461 acgcaacata accgaagcgc tgctctttat taaaatctct caaaaaggca gatttagaaa
     2521 tttgacctga aaccatttga ccactcagtt tatcgacatc tttaagttta atatgctctt
     2581 gtctctcctg cttaccttta cctgatgtaa accaaaaatt agcaaaggca tccttctcgc
     2641 tcaaaacacg caaagttcct gatcgttctt cattagctaa ttttatttct tcagttgatg
     2701 ttaaaacgcc gttatcataa cgctccacag taaagataaa aggcttatca acatccgctt
     2761 tggccacctt ataatccatg gcaaacaatt catctgtttt aattttttta gttcctgaat
     2821 cagatgattg tgtgatgtta aagccacacg cacttaacat aattacagca acaatgattg
     2881 ttcctagtaa cattttgtgc ttcattaggt ttcacctcta tttatttatt ttatcgtcga
     2941 ttagtttagc cttaaagaca tataattcat caaattttgc taaagattgc ttggtttttc
     3001 catcagctgc tttcactagc ttccggtttt gatccactga agaatctagc atcccttgag
     3061 tagaagtcac gatatttaga taacctatca ctacttcttt ttcacttaat gacgtttttc
     3121 ctgtgactac attatcaatc atagaagcca tttgatgctt atgttcaaca aaagaaggct
     3181 cccaagagga tgttgtgtat tgatttctga cacttaagta gacacgattt ttttcgacag
     3241 gcgatacact tatcgtgaaa taattagctt ctgatacagt tataatgact ggttcaaatg
     3301 atttaatata ctcgcctttt gtatagtgtt ccaacgtcag agcaattgct tttttcgtca
     3361 tatcattctt gttcagttta taatcaaaac ccatcatatg tgcgctggat tcctcagtag
     3421 aagcaagttt atgcattttt atgtagcttg ttttgggcgg tagtataaac ataaaatagg
     3481 ccaagctacc gatagcaata aataacacta cggtactaat caaaatattc tttttagaca
     3541 ttaaatctcc cccaaaaggc agtttttatt ttatggcttt tttaaattcg gcagtaaaaa
     3601 gatagagatc atcataagct tttaaggctg ctatttctgg cttaggttga ttatatttaa
     3661 caatgagagc cgcatcatct ttgcgtgaca atacacgttt gttgtctttt tgacgataag
     3721 cataaccaac gaccggtttg tttttgatcg ccgttttttc agaaagcgtt ccatccgtta
     3781 caaattgatc cttttctttt aaaacaacag gtttttctgg ccagttagta attgtaccat
     3841 catttactaa actaaattga taatcagtat gggtaacatc attttgatcg aagagaaatg
     3901 aaattttaaa gtcacctttt ttttcagcta aacccgttgc taaaggttca ctcttcccca
     3961 tacgttttcc gtttttgtaa tgtgtcaatg agacaaccaa tgtgcgttca gcatcggttt
     4021 tgttcaaacg ataatcagtt ccaattaaat gtagtacgcc tttccattca tctgccccca
     4081 ctaaatcgtt gctttttaaa taactttctt tcggagcttt cagtaataac caacttgtag
     4141 ctcctccgat aataagtact aatatagcga gtgataataa tgttttacgt ttcactgtgc
     4201 tcgctccttt ctgttccatt ttattatagc atagcacttc atcgttatta cttgaatttg
     4261 aacactgaaa agtagacttg agtaaaaacc ttgttacaac aacgtttatg agctaacaac
     4321 ttgtggttga acaccttatt ttaaacactt tttatacaat tactttgaac aatcgtatat
     4381 aattattttt agtaacaaca aacgattgaa taagaataga ggttgctgat gcgaaaaaaa
     4441 ttaagaaaca tgcttattta tataatttca tttctaatag cctttcaaat aataaaagca
     4501 gctagtaata ccttcttttt atataccgct gtagatgtaa cgattggcag cgaatttatc
     4561 acagttttaa tactatttgc attgacagca acatttgctg aaacaaccat tttaattatt
     4621 aagaaaaaga gctagaacaa tgaaaaaaaa attcatatcg ttttctctat tcaaaagtgt
     4681 ctgataatac taatcagaca ctttttttat gccctaaaac aactaattat tttatcccgt
     4741 accaagtaaa tcattcaatt aatagcagaa tcatatttaa attccaagtg tttttaagta
     4801 atataatatc ctcctattta ttaatactaa atcgcccttg aactacatct tctttcacaa
     4861 ataacaaact aaaagactag cttttaaata taaatagtct taaaatcacg aattaacggt
     4921 tttgttttag tttgatttta aatattaagt catggtatta tttgtttgta aacaggtttg
     4981 acaataagga ggagattgtt ttgaagaaaa tattattgct atctatttta acaattggtc
     5041 ttttaggatc agttgcaaca acagcacttg cttatgaagt aaacgttggt ggtggtacat
     5101 ggaattacgg aaatggtaag atttattctt ggtcaaatta ttatcataaa agtaaaacac
     5161 ataaatcttc tgtgactgtc ggtaattaca caacctctag tgattggata tctaaaaata
     5221 aatgggctaa agcctcagca aaaaaatcat tgtggagtac gtcacagttt tactggaata
     5281 caaaataaac aaaaatagcc tgtgaacttt tataggattc agattgtaga caaagtctcg
     5341 caaaactgct tttcatttga aaatgattta aacaactttg tctacgatct gagcaggttg
     5401 aatagaaatg ctgttatagt agaaacgacg agcgagtgac gacctagaac agtgtttatc
     5461 ttcagtctct agcctgtgaa cttttatagg ctttttatta ggagttatat aaatatgaaa
     5521 aaaatatggt ctttgttctt cgttatcggt ttaatcacta cattgttttt agcaactcaa
     5581 ctttataaag cgaaagaaga ttacgatagt atttatttaa caaaagcgaa cacagaagcc
     5641 atctcagtga ctgatcaacc ctcgcatcta agtgttaatg ctctaaacaa agaaattaat
     5701 tctttcacga aagcgaataa gattaatctt tatcgccaat tttataacga aagtgtgtca
     5761 agccaatata ataaaaccgt ctacgtttca attggtgata taaatggcgt ctcttcactc
     5821 tatgacctgt caaaaaatga ctctgaaaag ttgctcacga atggtacatt atcaactaaa
     5881 aataacgaaa ttaagttttt taatccaatt acaaacgtgc agctgactac tctcaaaaat
     5941 gctgaaaatg aagggttgac tggctactac ttcattcaat cagatactcc cgaaaaaata
     6001 aaaaaaacaa aagatttttt acttcaaaaa ggacttattt ttgaagatgt taaaaaaatt
     6061 tcacgaactg acttgataga tacgatcatg tcagaaaaca tgattgttcc tttaattata
     6121 tctattttaa tctttttaat tactttcatt ttcatatcct tttttgaagt gatgcaacgt
     6181 tttaaagaaa ttgccattta ccagacgtta ggttataaca atcgtcaaat aaaggtagcg
     6241 ttcatccatc aaaaattacg aatcattatt tatatgatat taatcagcat ttgtgctatt
     6301 tttgcctggt gtttgtggtt taatggtggc attcaaatag tagaattatt tatcttctcg
     6361 actgttttca tagtggttta ccttttaagc gtattattca tttactatct gtgtttaatc
     6421 agcatgaaat tcatcacaat taacttgatg atcaaaggta aaaagcccta tcataaattg
     6481 ttaattgttc aacaaacctt taaattttta tgcctcgcaa tcttgctgat attatcaata
     6541 cacacgacta atgagattag gcaattaaat tatcaaatcg ctcaattaga aacctttaac
     6601 acggttaaaa agttcaatac tactcatatt tctatcacaa ccgctagtaa ggacgctgaa
     6661 gaagttgtag aagataaaat atattcatta aatacggcgc ttgataaaaa aggcagtttt
     6721 atactctctc ctagtaattt ttatctgaat cctgcagaat ttggtgataa tagtgatgaa
     6781 ctaccttacg tattggtttc gttaaattat tttaaagcaa atcctcaact agggatcgat
     6841 tatgctcaat taaaacaatc gttaaacaat aaaggcgatt tagttgctct ctttccagat
     6901 aattacaggt tagacaaaga tgttttcaaa acgaatttga ctgaattact aatcctttct
     6961 aaaaagacac cacctcacgt tttgtcaaaa acataccctc atcaaaaaat acctatttac
     7021 gactacactt tcactaaatt ctctgataca aatatagaat cgcctatctt cgttgttttt
     7081 gacagtaaaa aagcgtcacc tagcacgatt ttaagcgctt attctaatgg tttagctttt
     7141 ttaaacttag gagaaaatga tgccttcaaa aaattaacgc ctcttctaga aaaatataaa
     7201 ttagttgata atatacgaaa tataaacaat gtaaaaaaaa cagtcacaca acaaattgtc
     7261 ttgttgcaaa atgagttaaa tgtgcgtatc tttactttac taactttaac taccgtcttg
     7321 ttaattacct tgttttttat caccaattta tatattgagt atcaacaaaa aatgttagcg
     7381 gtgaaaaaat gtcatggcta taatttatta caacgtcata ttttctttct gctcacagaa
     7441 ttactggttt tacttgctgt tttagtgtcc gtctctttaa tgattactct taatagtaga
     7501 acgcttatct ttatagcagc tgctttaatg attacattta ttatcacact attttttgtt
     7561 atagtgtttg aaaaaaatag tattaataag gtaatgaaag gagagtaata atgattagaa
     7621 taactaatat ttcgaaaaaa ttccaaggaa atagtgtcgt ttctaatttc agtgaacaca
     7681 tcttagaaaa tgaaatggta gcaataatgg ggcccagtgg ttcagggaaa acaaccttat
     7741 taaatatgat tgctggtatt gaagaatgtg atagcggtac tgtcacaatt aataacaata
     7801 aaaacatatc aaacattttc aaactcagat atgatattgc tctcctgttc caaaattatg
     7861 gcttaattga ccagataacc gttgaagata atttgatgat tgctatgaaa ttctcacaag
     7921 aaaaaaataa aaatgaagca attaaagctg tcttgattga atttaattta tacgataaaa
     7981 gaaaacaaaa agtctttaca ttaagtggtg gtgaacaaca gcggattgct ctagcaagaa
     8041 tcatgttaaa accacacact attatccttg cagatgaacc cactggctca ctagatgcta
     8101 ttaataaaga attcgtgatg tctcaactta ttaaatttaa aaaagctggt aaaacgattg
     8161 ttgtcgttac tcacgatcaa actatttctg ctttgtgtga tcgtacaatc actttataga
     8221 aagaggttta ttatgaaaaa aatatttttc ctaactattg cgttggtggt attaatttta
     8281 atattttcgt attcgactaa agatattgct gctgtcaatc gctttaatcc ctttgtaaaa
     8341 gaaacaacca gttatgccaa agtcccaaca ggtactcaaa tatacaaagg aataacactg
     8401 attgataaca aaaagaacac cgttattgtg gactttggtg gttatgatcc tgagggtgtt
     8461 tatgtgaaag taaaacacaa acgtaatttt gttaagcatg ttgattatat taccgcagaa
     8521 aaaataccag aaaccatcag aaaagatatc actcattaat aggtttctag caattgaaaa
     8581 agagtgtcag aatagtattc tgacactctt tttatttgat actatctttc caatttaaaa
     8641 caaaaaattt aaactcttgc tttaattagt ttcttaaaat tttatccatt tttttacctt
     8701 ttgctaattc atcaattaac ttatctaaat aacgaatttg ttgcatcaaa ggttgttcaa
     8761 tttcctctac gcgaatgcca caaacaaccc ctttaattaa atccgttttt ggattcattt
     8821 gcggcgcctc agcaaaaaat tgcttaaaat caacagcatc ttccaattgt tggtttaaac
     8881 tggttgtatc atatcctgtt agccagaaaa taattttgtc tacttcttct tttgttcgtt
     8941 gtttacgttc tgcctttttg atgtataacg ggtaaacact tgcaaaactt gttgtataga
     9001 tacgatgatt ttccaattct gaatccctcc tttatccaat aattttatta taacaaaaaa
     9061 agataaactt caatttaatt aaagtttatc ttttcttagt tttaatggat tatttattcc
     9121 agttctcagg atctttattc cattcacgta atgtttctaa ctcagcttcc ttcacataac
     9181 catcttctgc cgcaacttct agcaatgttg agtagtttgt taacgtcagt aatggaatgt
     9241 tagcagcctc taaattacgt tgggcagctg ccagctcgta agtgaaaatt gcggcgattg
     9301 caatgacttc agagcctgcc tcttcaaccg ctttagcagc tttcaaacat gagcctgctg
     9361 ttgaaattaa gtcttcaatc attacaactt ttttacctgg cgcaagggga ccttcaattt
     9421 gtttcccttt accatgtttt ttaggttcag aacgaacata aatcattggc aaatctaatt
     9481 cttccgcaat aaacgcggca tgagggatac ctgcagttgc agtccctgca atgacatcca
     9541 catcaggata gtttgcttta atttcagcgg ctaaaccttt tgcgattgcg cgtcgaacat
     9601 tcgggaatcc tagtgtcata cggttatcac aataaatagg tgatttaatg ccagatgccc
     9661 atgtaaacgg ctcctccggt ttaagaaaaa cggcttctat ttctaataaa tgtttggctg
     9721 tttgtttagc gattgtcatt gttaacactc cattctgata agattgcatg atatgctgcg
     9781 acagcatcat cagcttgtgt aatcggacga ccgaccacaa tatgtgttga accatttgct
     9841 cgtgcttgtg caggtgtcat aatacgtcgt tgatcaccta ctgttgcaga taatgggcga
     9901 atacctggtg ttacgcgcaa aaaattagcg gctgttgctt caccgattgc ttttgcttca
     9961 tgtgctgaac aaacaacacc atctaaacct gcggcaaaag tcgttttagc ataatgtgtg
    10021 actgattcca ataatggcac tttaattaat tggtctgacc acatttcttc ttcagatgtt
    10081 gatgtaagtt gtgtgacggc aattaattgt ggcacatgcc ccgcggtaga tccttgaatt
    10141 aaaccttctt ttgcagctgc catcatccgt tgacctcctg ctgcatgcac attgaccatg
    10201 tccacaccta agcgtgctaa attagccatt gctcgttgta cagtattcgg aatatcatgt
    10261 aattttaaat ccaagaaaat ttcatggc
//
