LOCUS       c00060_NZ_BBIR..       11047 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_BBIR01000099.1 Pseudomonas japonica NBRC 103040 = DSM 22348
            strain NBRC 103040, whole genome shotgun sequence.
ACCESSION   NZ_BBIR01000099
VERSION     NZ_BBIR01000099
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 16:47:01
            Original ID  :: NZ_BBIR01000099.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 6866
            Orig. end    :: 17913
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..11047
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[11866:12913](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6047
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..11047
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..11047
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     CDS             complement(491..1324)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091:glutamine-binding lipoprotein glnH (Score: 100.7;
                     E-value: 1.2e-30)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg60_7"
                     /transl_table=11
                     /translation="MIGLRQLPWLAALLVSGGLSAEPLLERAVDRGVLNVCSGDEFPPF
                     KFLDAQGRPAGLIPDLIDDLHRQLATATGKALTLRLVQVNPVNRILFLQQGRCDLLMTS
                     MLDTPARREQVDIASPGFYQSAATVFAPKASVLADWSSLRGKTLCAPATSVWIRPLEER
                     YGVRFISFNGSAEVRKAVADQRCLGALGDDVLYRALAATPGWEDYEVKLPGQDPAPWGI
                     AVRKGQPQLLSALSRIVEGWHAEGLIIALERHYGLAPSPWLVEQQQARQAVARHD"
     PFAM_domain     complement(557..1225)
                     /aSDomain="SBP_bac_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00497.22"
                     /description="Bacterial extracellular solute-binding
                     proteins, family 3"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_7_0001"
                     /evalue="6.50E-28"
                     /label="ctg60_7"
                     /locus_tag="ctg60_7"
                     /protein_end="256"
                     /protein_start="33"
                     /score="97.8"
                     /tool="antismash"
                     /translation="LNVCSGDEFPPFKFLDAQGRPAGLIPDLIDDLHRQLATATGKALT
                     LRLVQVNPVNRILFLQQGRCDLLMTSMLDTPARREQVDIASPGFYQSAATVFAPKASVL
                     ADWSSLRGKTLCAPATSVWIRPLEERYGVRFISFNGSAEVRKAVADQRCLGALGDDVLY
                     RALAATPGWEDYEVKLPGQDPAPWGIAVRKGQPQLLSALSRIVEGWHAEGLIIALERHY
                     G"
     CDS             complement(1321..1995)
                     /locus_tag="ctg60_8"
                     /transl_table=11
                     /translation="MTSQTPPADLLLLVQQFEQDVASAHQLLQRHQALSANEAGNLSLR
                     LPGQERLLIAAFNGPDAGRAAVLDFDLEQSQGRLNDNLREVAALHVAIYRQRPQVRAVI
                     HTHSPYLSAFAIAGRPLRAHASQLLGVLDEDQEIPLTAWGPRYAPEPVIAALQAHPRAP
                     ATLLANHGPFAWSERDALAATRLLINLEESAHLTWLAAQLGTPQPFPAGAAARSRQGWV
                     AS"
     PFAM_domain     complement(1408..1941)
                     /aSDomain="Aldolase_II"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00596.23"
                     /description="Class II Aldolase and Adducin N-terminal
                     domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_8_0002"
                     /evalue="3.40E-22"
                     /label="ctg60_8"
                     /locus_tag="ctg60_8"
                     /protein_end="196"
                     /protein_start="18"
                     /score="79.3"
                     /tool="antismash"
                     /translation="QDVASAHQLLQRHQALSANEAGNLSLRLPGQERLLIAAFNGPDAG
                     RAAVLDFDLEQSQGRLNDNLREVAALHVAIYRQRPQVRAVIHTHSPYLSAFAIAGRPLR
                     AHASQLLGVLDEDQEIPLTAWGPRYAPEPVIAALQAHPRAPATLLANHGPFAWSERDAL
                     AATRLLINLEESAHL"
     CDS             complement(2022..4706)
                     /gene_functions="transport (smcogs)
                     SMCOG1082:TonB-dependent siderophore receptor family
                     (Score: 196.5; E-value: 1.9e-59)"
                     /gene_kind="transport"
                     /locus_tag="ctg60_9"
                     /transl_table=11
                     /translation="MHQRFTLASLACVLAIQGLPATVFADSATAPASQQQQVEYDIPAG
                     SLEDVLLAISQRSSKAIVYDQAQVAGLHGPAVRGRFTAQQALDTALRGSGLGHSSSPSG
                     AVRIHRLDDSAAAAPLTPAAAPRLGTVQVIGTRRSDIGALQASAPVDIIQGEALRRTGH
                     DSLAKALETLVPSVSYPQINGTDGVSAQRPVLLRGLAADQVLVLVNGKRRHASAFVNTK
                     ATLGRGSQAVDLSTLPVAAVDHIELLRDGASAQYGSDAIAGVINIVLKDRDQGGEAQAS
                     TGQYSKGDGFSRSLSGWQGFSLPGDGYLTVSAEGVKSERTSTDGADLRRFYPAGDPREA
                     NAERHWRYGSPGLDDWKLGANAGAFITEHTELYGFATYQDRTAESQAVYRRPIDANNIV
                     SVYPDGFLPLLDVRSRDAAGTAGIKYEDDALGRFDLSATYGRNRIDYHLGDSLNASLGP
                     ASPRSFDAGALINDQAQLSLDHVKEFAVGYSAAPLVFSSGLAWRHEGYEVLAGDTASWV
                     QGRVLPSRAGGAQGFPGTRPSDEGDYSRKVLGAYLGLEQQLTQALQLGVAARSEHYDDF
                     GNTSTGKLSLRYDFNEHFGLRSTLSSGYRAPTLGQIGTSATQTEFLAGDPTAYQVGTLS
                     VDTGVARALGAQDLKPEKSRNISVGLVWQPVEDASLTLDAYQIRIKDRIALSETLRGST
                     VNQILASQGYGNYSGVSFFTNALDTRTHGLDLIGHYRLALPQVGQVTFNAGFNWSKTQV
                     TDIQDNPGPLSGSGVILINREALSYVQSASPQSKLVLGADWRQGPWSAAFTTLRYGRYT
                     LDSNTGPAFDQTFGSQWVSNASLSYDVDSALTLTAGANNLFDSYPDKIDVANRFVGGRV
                     QYQSISPAGAEGAYYYLKANYHF"
     PFAM_domain     complement(2031..3629)
                     /aSDomain="TonB_dep_Rec"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00593.26"
                     /description="TonB dependent receptor"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_9_0004"
                     /evalue="2.40E-43"
                     /label="ctg60_9"
                     /locus_tag="ctg60_9"
                     /protein_end="892"
                     /protein_start="359"
                     /score="149.8"
                     /tool="antismash"
                     /translation="ANAGAFITEHTELYGFATYQDRTAESQAVYRRPIDANNIVSVYPD
                     GFLPLLDVRSRDAAGTAGIKYEDDALGRFDLSATYGRNRIDYHLGDSLNASLGPASPRS
                     FDAGALINDQAQLSLDHVKEFAVGYSAAPLVFSSGLAWRHEGYEVLAGDTASWVQGRVL
                     PSRAGGAQGFPGTRPSDEGDYSRKVLGAYLGLEQQLTQALQLGVAARSEHYDDFGNTST
                     GKLSLRYDFNEHFGLRSTLSSGYRAPTLGQIGTSATQTEFLAGDPTAYQVGTLSVDTGV
                     ARALGAQDLKPEKSRNISVGLVWQPVEDASLTLDAYQIRIKDRIALSETLRGSTVNQIL
                     ASQGYGNYSGVSFFTNALDTRTHGLDLIGHYRLALPQVGQVTFNAGFNWSKTQVTDIQD
                     NPGPLSGSGVILINREALSYVQSASPQSKLVLGADWRQGPWSAAFTTLRYGRYTLDSNT
                     GPAFDQTFGSQWVSNASLSYDVDSALTLTAGANNLFDSYPDKIDVANRFVGGRVQYQSI
                     SPAGAEGAYYYLKANY"
     PFAM_domain     complement(3915..4271)
                     /aSDomain="Plug"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07715.17"
                     /description="TonB-dependent Receptor Plug Domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_9_0003"
                     /evalue="6.40E-20"
                     /label="ctg60_9"
                     /locus_tag="ctg60_9"
                     /protein_end="264"
                     /protein_start="145"
                     /score="71.8"
                     /tool="antismash"
                     /translation="ASAPVDIIQGEALRRTGHDSLAKALETLVPSVSYPQINGTDGVSA
                     QRPVLLRGLAADQVLVLVNGKRRHASAFVNTKATLGRGSQAVDLSTLPVAAVDHIELLR
                     DGASAQYGSDAIAGV"
     CDS             5001..6047
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg60_10"
                     /sec_met_domain="AfsA (E-value: 3.4e-23, bitscore: 71.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLQHPDPTTFDSAVVLAATVEENTVPRGMVHRAAVSEVFLTGA
                     QRVAEGHFRCLAQLPRTHSYFSEHTHRDAHYDTLLLLEVFRQASIYVSHAFLDVSTSDK
                     FIYLDSDTSIVEREALVVGARPASALIDVRVVGEYLRHGQRSGVTLDMTLSVNGRVAAR
                     HDGMSIRWMSDTAWNKIRTPALAASAAQHDPLAQSPAPLAPFQVGRHWLSNVVLGREIV
                     VEDGHLVAALIVDQSNPAIFDHPLDHIPGMLLLEGFRQLALVGAKRQLGLAPDELLLGR
                     ARVAFTRFGEFGLATQVKANLDSLRVLDDGRAVVNLSVEQGGAQLAHAEVELQRLFQPL
                     ALAAAGGA"
     PFAM_domain     5091..5492
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_10_0005"
                     /evalue="1.40E-22"
                     /label="ctg60_10"
                     /locus_tag="ctg60_10"
                     /protein_end="164"
                     /protein_start="30"
                     /score="80.1"
                     /tool="antismash"
                     /translation="MVHRAAVSEVFLTGAQRVAEGHFRCLAQLPRTHSYFSEHTHRDAH
                     YDTLLLLEVFRQASIYVSHAFLDVSTSDKFIYLDSDTSIVEREALVVGARPASALIDVR
                     VVGEYLRHGQRSGVTLDMTLSVNGRVAARH"
     PFAM_domain     5619..5993
                     /aSDomain="AfsA"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03756.15"
                     /description="A-factor biosynthesis hotdog domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_10_0006"
                     /evalue="2.60E-18"
                     /label="ctg60_10"
                     /locus_tag="ctg60_10"
                     /protein_end="331"
                     /protein_start="206"
                     /score="66.3"
                     /tool="antismash"
                     /translation="VGRHWLSNVVLGREIVVEDGHLVAALIVDQSNPAIFDHPLDHIPG
                     MLLLEGFRQLALVGAKRQLGLAPDELLLGRARVAFTRFGEFGLATQVKANLDSLRVLDD
                     GRAVVNLSVEQGGAQLAHAEV"
     CDS             6047..6733
                     /locus_tag="ctg60_11"
                     /transl_table=11
                     /translation="MSAIERVWFDFGGVLSPPIDALFAQYEVKTGLAPAVLQRAFAEVA
                     EALGVPPLAPIENALLSEDEWGRRVELALSRQEPPADLSRARLRRFGEQWFAGVQANAA
                     MVAAARKLKESGFAIGILTNNVVEWEPYWRAIVGLDEVADLIVDSSKEGCRKPDAQFFD
                     IARQRAGLPAAANLLIDDLAENVAAAAALGWRTVHFRDNRQALGDITHATGVCVFSVQQ
                     PEGAPA"
     aSDomain        6344..6640
                     /aSDomain="TIGR01509"
                     /aSTool="tigrfam"
                     /description="HAD-SF-IA-v3: HAD hydrolase, family IA,
                     variant 3"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg60_11_0001"
                     /evalue="1.10E-09"
                     /identifier="TIGR01509"
                     /label="ctg60_11"
                     /locus_tag="ctg60_11"
                     /protein_end="198"
                     /protein_start="99"
                     /score="36.6"
                     /tool="antismash"
                     /translation="QANAAMVAAARKLKESGFAIGILTNNVVEWEPYWRAIVGLDEVAD
                     LIVDSSKEGCRKPDAQFFDIARQRAGLPAAANLLIDDLAENVAAAAALGWRTVH"
     PFAM_domain     6506..6655
                     /aSDomain="Hydrolase_like"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13242.8"
                     /description="HAD-hyrolase-like"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_11_0007"
                     /evalue="3.50E-05"
                     /label="ctg60_11"
                     /locus_tag="ctg60_11"
                     /protein_end="203"
                     /protein_start="153"
                     /score="23.9"
                     /tool="antismash"
                     /translation="CRKPDAQFFDIARQRAGLPAAANLLIDDLAENVAAAAALGWRTVH
                     FRDNR"
     CDS             6733..7971
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 300.2; E-value: 3.6e-91)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg60_12"
                     /sec_met_domain="p450 (E-value: 4.9e-13, bitscore: 38.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIALDSLPGAAARLADIDITHIANLDAAPAYRPLPAEGGFQRIL
                     LPSGHSAIHLTRYAEVQALLLDNRAIRAPCNEEDGPSFLPTITPPELLLNNDIPDHGRL
                     RKVVARDFSPSGVAVLREAVRQVTHARIDLLRQQGRGADLFSLVLEQVPSTVDCQLLGI
                     PLADRDYYRPLSHTVQIASDHDVPDLLRQFWGVYDYLTDLVTGARPSLPDGLIQRFIAA
                     RNDSEPPLSDKELVGILLGVLIGGDQNILSVLTKAVYVLLAQPVLYRSLVDDPGLIPSA
                     VEELLRLLPLGTISTFPRLASSDIEGPWGLIPAGSVIYADAFAANRDPEVFSDPLAIRL
                     DRQEARHLQFGYGMHNCMGAALVRLEIATVLEILVERLPDLRLDIAAEQVPWVEGIILR
                     RPTRLPVRGFTSL"
     PFAM_domain     7570..7863
                     /aSDomain="p450"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00067.24"
                     /description="Cytochrome P450"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_12_0008"
                     /evalue="5.90E-10"
                     /label="ctg60_12"
                     /locus_tag="ctg60_12"
                     /protein_end="377"
                     /protein_start="279"
                     /score="38.6"
                     /tool="antismash"
                     /translation="SAVEELLRLLPLGTISTFPRLASSDIEGPWGLIPAGSVIYADAFA
                     ANRDPEVFSDPLAIRLDRQEARHLQFGYGMHNCMGAALVRLEIATVLEILVER"
     CDS             7988..9523
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 318.1; E-value:
                     1.2e-96)"
                     /gene_kind="transport"
                     /locus_tag="ctg60_13"
                     /transl_table=11
                     /translation="MNSKLSDNALLGTILLAIFVVPSSISGTALALPAIGNDIQASLAQ
                     LQWVVNAFNLTFACFTLAWGALADRLGRKRCFVVGAGLYVLASVISALASQAWVLDAGR
                     ALAGIGAAAIFSCGIAILSTHFDGAARLRAFALFGTVAGLGVSLGPTLSGLLIEHFGWR
                     AIFWAHLLTLGLVLLGTPLITRDGQLAERTARFDLLGSLLFILALLALMVAIVQGSQWG
                     WRSQGVLGLVALSAVLLVLFAWQERRHRQPMLDLSLLTSGPFVGLALVTVAASFGFVTL
                     LTYLPSYLFGVLRLAPTQAGLAMLLLTVPMLFCPILAGKWAARGVSAVAILKASLLALL
                     AGVVGLALLAQAHAALWHLAAPLLLVGVGMGLSAGLVDGLALKTVPEQKAGMAAGLLNT
                     FRLGSEAIAVAVYGSLLATLLDDQLRQELARLSSDPATVRGWIDSVAAGNLEGALRGVA
                     QPELVGDLLRGGYDAAFHGVLWFLAAVIVVLILVIGALLRPLRQQERGALAVQ"
     PFAM_domain     8027..9196
                     /aSDomain="MFS_1"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF07690.18"
                     /description="Major Facilitator Superfamily"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_13_0009"
                     /evalue="1.10E-48"
                     /label="ctg60_13"
                     /locus_tag="ctg60_13"
                     /protein_end="403"
                     /protein_start="13"
                     /score="166.1"
                     /tool="antismash"
                     /translation="ILLAIFVVPSSISGTALALPAIGNDIQASLAQLQWVVNAFNLTFA
                     CFTLAWGALADRLGRKRCFVVGAGLYVLASVISALASQAWVLDAGRALAGIGAAAIFSC
                     GIAILSTHFDGAARLRAFALFGTVAGLGVSLGPTLSGLLIEHFGWRAIFWAHLLTLGLV
                     LLGTPLITRDGQLAERTARFDLLGSLLFILALLALMVAIVQGSQWGWRSQGVLGLVALS
                     AVLLVLFAWQERRHRQPMLDLSLLTSGPFVGLALVTVAASFGFVTLLTYLPSYLFGVLR
                     LAPTQAGLAMLLLTVPMLFCPILAGKWAARGVSAVAILKASLLALLAGVVGLALLAQAH
                     AALWHLAAPLLLVGVGMGLSAGLVDGLALKTVPEQKAGMAAGLLNTFRLG"
     CDS             9778..10287
                     /gene_functions="regulatory (smcogs)
                     SMCOG1126:extracytoplasmic-function sigma-70 factor (Score:
                     123.7; E-value: 1.2e-37)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg60_14"
                     /transl_table=11
                     /translation="MNGQNLLKQQTVTRIFQQHYLWLCSRLAFRTGCNHSAEDIAAETF
                     LRVWMLPDPTAIREPRALLTTIAQRLMYETWRRRDLEKAYLQSLSLVPEELHPSPQEQL
                     MLIQSLVAIDRLLEGLSRQARSVFVLSQLEGLTYVQIQQRLGLSLGRIHQLMAQALRCC
                     YLGLEE"
     PFAM_domain     9823..10017
                     /aSDomain="Sigma70_r2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF04542.16"
                     /description="Sigma-70 region 2"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_14_0010"
                     /evalue="6.70E-10"
                     /label="ctg60_14"
                     /locus_tag="ctg60_14"
                     /protein_end="80"
                     /protein_start="15"
                     /score="38.7"
                     /tool="antismash"
                     /translation="FQQHYLWLCSRLAFRTGCNHSAEDIAAETFLRVWMLPDPTAIREP
                     RALLTTIAQRLMYETWRRRD"
     aSDomain        9823..10260
                     /aSDomain="TIGR02937"
                     /aSTool="tigrfam"
                     /description="sigma70-ECF: RNA polymerase sigma factor,
                     sigma-70 family"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg60_14_0002"
                     /evalue="1.10E-15"
                     /identifier="TIGR02937"
                     /label="ctg60_14"
                     /locus_tag="ctg60_14"
                     /protein_end="161"
                     /protein_start="15"
                     /score="55.6"
                     /tool="antismash"
                     /translation="FQQHYLWLCSRLAFRTGCNHSAEDIAAETFLRVWMLPDPTAIREP
                     RALLTTIAQRLMYETWRRRDLEKAYLQSLSLVPEELHPSPQEQLMLIQSLVAIDRLLEG
                     LSRQARSVFVLSQLEGLTYVQIQQRLGLSLGRIHQLMAQALR"
     PFAM_domain     10108..10266
                     /aSDomain="Sigma70_r4_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF08281.14"
                     /description="Sigma-70, region 4"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg60_14_0011"
                     /evalue="1.90E-11"
                     /label="ctg60_14"
                     /locus_tag="ctg60_14"
                     /protein_end="163"
                     /protein_start="110"
                     /score="43.6"
                     /tool="antismash"
                     /translation="VAIDRLLEGLSRQARSVFVLSQLEGLTYVQIQQRLGLSLGRIHQL
                     MAQALRCC"
ORIGIN
        1 ggccagcggc aggaggatgt ggcgccacag cgacaggccg tgcaggccca gcgccgcggc
       61 gctttcatgg atgccgcggg gcaccgtgtc gataccggcg cgcagcgctt cgatctggaa
      121 ggcgccgctg tacagggcga tggcgatgat cgcccagccg gtgccgccga gcaggcgcac
      181 cgggctgccg tcgtcgccga cggtcagtgg cagcagggcg ccgaggccga agtagaagaa
      241 gaacagttgc accagcagcg gggtgctgcg ccacagcgcg acgtagccgt cgaccaggcc
      301 gcgcagccag cgtctggggc tgcgctgggc ggccacaccg agggcgccga tcagcagcga
      361 ggcgaggccg gcgaccacgg ccatgagcag ggtcaggccc agcccggcga gcaggcgctc
      421 gcggtcggtc gggtcccaca gcacgctgag ggtcaggccg tggtcttgca gccacaggcg
      481 caggtcgggc tcagtcatgc cgggccaccg cctggcgtgc ctgctgctgc tcgaccagcc
      541 aggggctggg cgccaggcca tagtggcgct ccagggcgat gatcaggccc tcggcgtgcc
      601 agccctcgac gatccgcgag agtgcgctga gcaactgcgg ctggcccttg cgcacggcga
      661 tgccccaggg ggccgggtcc tggccgggga gtttgacttc gtagtcctcc cagcccgggg
      721 tggccgccag ggcgcggtac agcacatcgt cgccgagggc gccgaggcag cgctggtcgg
      781 ccaccgcctt gcgcacttcg gcactgccgt tgaaggaaat gaaacgcacg ccgtagcgct
      841 cttccagcgg tctgatccag acgctggtgg ccggcgcgca cagggtcttg ccgcgcaggc
      901 tcgaccagtc cgccagcacg ctggccttgg gcgcgaacac cgtggcggcg gactggtaga
      961 agccggggct ggcgatgtcc acctgctcgc gccgggcggg ggtgtcgagc atcgaggtca
     1021 tcagcaggtc gcaacggccc tgctgcagga acaggatgcg attcaccgga ttgacctgca
     1081 ccagacgcag cgtcagcgcc ttgccggtgg cggtcgccag ctggcggtgc aggtcgtcga
     1141 tcaggtcggg gatcagcccg gcggggcgtc cctgcgcatc gaggaacttg aacggcggga
     1201 actcgtcgcc gctgcacacg ttgaggactc cgcggtccac cgcgcgttcc agcagcggct
     1261 cggcgctgag cccgccgctg accagcagcg cggccagcca gggcaattgg cgcaggccga
     1321 tcatgaggcc acccagccct gtcgcgagcg cgccgcggcg ccggccggga agggttgcgg
     1381 cgtgcccagc tgggccgcga gccaggtcag gtgggccgac tcttcgaggt tgatcagcag
     1441 gcgggtggcg gccagggcat cgcgctcgct ccaggcgaac ggcccgtggt tggccagcag
     1501 cgtcgctggt gcccgcggat gcgcctgcag cgcggcgatc accggttccg gcgcatagcg
     1561 cgggccccag gcggtgaggg ggatttcctg gtcttcatcg aggaccccga gcaactggct
     1621 ggcatgcgcg cgcagcggtc ggccggcgat ggcgaaggcg ctcaggtacg gcgaatgggt
     1681 atggatcacc gcgcgcacct ggggccgctg gcggtagatg gcgacatgca gcgcggcgac
     1741 ttcgcgcagg ttgtcgttga gccggccctg gctctgttcg aggtcgaagt cgagcacggc
     1801 ggcgcggccg gcgtcggggc cgttgaaggc ggcgatcagc aggcgctcct ggccgggcag
     1861 gcgcaggctg aggttgcccg cttcgttggc cgagagcgcc tggtggcgct gcagcaactg
     1921 gtgggcgctg gcgacgtcct gttcgaattg ctggacgagc agcagcaggt ccgcgggggg
     1981 tgtctggctg gtcatgacgg tcttcctgtt cagggggcgg gtcagaagtg gtagttggcc
     2041 ttgaggtagt agtacgcacc ctcggcgccg gccggcgaga tgctctggta ctgcacgcga
     2101 ccgccgacga agcggttggc cacgtcgatc ttgtccgggt agctgtcgaa caggttgttg
     2161 gccccggcgg tcagggtgag ggccgaatcg acgtcatagg acaggctggc gttgctcacc
     2221 cactggctgc cgaaggtctg gtcgaaggcc gggccggtgt tggagtcgag ggtgtagcgg
     2281 ccgtagcgca gggtggtgaa cgccgccgac cagggcccct ggcgccagtc ggcgccgagc
     2341 accagcttgc tttgcgggct ggcgctctgg acgtagctca gggcctcgcg gttgatcagg
     2401 atgaccccgc tgccgctgag cgggccgggg ttgtcctgga tatcggtgac ctgggtcttg
     2461 ctccagttga agccggcgtt gaaggtcacc tggccgacct ggggcagggc caggcggtag
     2521 tgcccgatca ggtccaggcc atgggtgcgg gtatcgaggg cgttggtgaa gaaactcacc
     2581 ccggagtagt tgccgtagcc ctggctggcg aggatctggt tgaccgtgct gccgcgcagg
     2641 gtctccgaca gggcgatgcg gtccttgatg cggatctggt aggcgtcgag ggtcaggctg
     2701 gcgtcctcga ccggctgcca gaccaggccc acggaaatgt tccgcgactt ctccggtttc
     2761 aggtcctggg cgcccagcgc gcgggccacg ccggtgtcga cgctgagggt gccgacctgg
     2821 taggcggtgg ggtcgccggc gaggaactcg gtctgggtcg ccgaggtgcc gatctggccg
     2881 agggtcggcg cgcgatagcc gctgctcagg gtggagcgca ggccgaagtg ctcgttgaag
     2941 tcgtagcgca gcgagagctt gccggtgctg gtgttgccga agtcgtcgta gtgttcgctg
     3001 cgcgccgcca cgcccagttg cagggcctgg gtcagttgct gttcgagccc caggtaggcg
     3061 ccgagcacct tgcgcgaata gtcgccttca tcgctcggcc gggtgccggg aaaaccctgg
     3121 gcgccaccgg cccgggacgg caggacgcgg ccctggaccc aggacgcggt gtccccggcc
     3181 agcacttcgt agccctcgtg gcgccaggcc aggccgctgg agaacaccag cggcgcggcg
     3241 ctgtagccga cggcgaattc cttgacatgg tcgaggctca gttgggcctg gtcgttgatc
     3301 agcgcgccgg catcgaagct gcgcgggctg gcgggaccga ggctggcgtt gagcgagtcg
     3361 ccgaggtggt aatcgatgcg gttgcggcca taggtcgcgc tgaggtcgaa gcggcccagc
     3421 gcgtcatcct cgtacttgat accggcggtg ccggcggcat cgcgcgagcg cacgtcgagc
     3481 aggggcagga agccgtcggg atagacactg acgatattgt tggcgtcgat cggccgacgg
     3541 tacacggcct gggactcggc ggtgcggtcc tgataggtgg cgaagccgta cagctcggtg
     3601 tgttcggtga tgaaggcgcc ggcgttggcc cccagtttcc agtcatccag ccccggcgag
     3661 ccgtagcgcc agtgccgctc ggcgttggct tcgcgggggt cgcctgccgg gtagaagcgg
     3721 cgcaggtcgg cgccgtcggt ggaggtgcgt tcgcttttca cgccttcggc gctgacggtc
     3781 aggtagccgt cgcccggcag gctgaagccc tgccagcccg acagcgagcg gctgaaaccg
     3841 tcgcccttgc tgtactggcc ggtgctggcc tgggcctcgc cgccctggtc gcggtccttg
     3901 aggacgatgt tgatcacccc ggcgatggca tccgagccgt attgcgccga ggcgccgtcg
     3961 cgcagcagtt cgatatggtc gacggcggcc accggcagcg tcgacaggtc caccgcctgc
     4021 gagccgcgac cgagcgtggc cttggtgttg acgaaggccg aggcgtgccg gcgcttgccg
     4081 ttgaccagca ccagcacctg gtcggcggcg aggccacgca gcagcaccgg gcgctgcgcg
     4141 gaaacgccgt cggtgccgtt gatctgcggg tagctgaccg agggcaccag ggtttccagg
     4201 gccttggcca ggctgtcgtg gccggtgcgc cgcagcgcct cgccctggat gatgtccacc
     4261 ggcgcgctgg cctgcagggc gccgatgtcg ctgcgccggg tgccgatcac ctggaccgtg
     4321 cccaggcgtg gcgcggccgc cggggtcagc ggcgcggcgg cggcgctgtc gtccaggcgg
     4381 tggatgcgca ccgcgccact ggggctgctg ctgtggccca ggccgctgcc gcgcagggcg
     4441 gtgtccagcg cctgctgcgc ggtgaagcgg ccccgcacgg cgggaccgtg cagaccggcg
     4501 acctgcgcct ggtcatagac gatggccttg ctgctgcgtt gcgaaatcgc cagcagcacg
     4561 tcttcgaggc tgccggcggg aatgtcgtac tcgacctgtt gctgctggct ggcgggggct
     4621 gtggcgctgt cggcgaacac ggtggcgggc aggccctgga tggccaggac gcaggccagg
     4681 gaggcgaggg tgaatcgttg gtgcatgaat gactccagat cggttcagcg cgggctgttg
     4741 tttccgacct tgagcgatga gcgcccggga ttttcagcgc cgcgattaga acatctggtt
     4801 gcatcgatat aacggctgcg accctcgggc cagagctgtc gcgcgggtat aagcacgtaa
     4861 ccaaacgatc tgtacgggca gtgaaaactt tgcgcctccg gcccacacac aagcggaacc
     4921 ggtcgagccg acacgccagc tacggagtgt gtggtcgacg gtgccgaacc ccttgcgtac
     4981 aacctgaaga ggcctgctcc atgaccagct tgcagcatcc tgatccgacg accttcgact
     5041 ccgccgtggt gctggccgcc actgtcgagg aaaacaccgt gccgcgcggc atggtccacc
     5101 gcgcggcggt gagcgaggtg ttcctgaccg gcgcccagcg cgtcgccgag ggccatttcc
     5161 gctgcctggc gcaactgccg cggacccaca gctacttcag cgagcacacc caccgcgacg
     5221 cgcactacga cacgctgttg ctgctggagg tgtttcgcca ggcctccatc tatgtgtccc
     5281 acgccttcct cgacgtgagc accagcgaca agttcatcta cctcgacagc gacacctcga
     5341 tcgtcgagcg cgaagccctg gtggtcggtg cgcggccggc gtcggcgctg atcgacgtgc
     5401 gggtggtggg cgagtacctg cgccacggcc agcgcagcgg cgtgaccctg gacatgaccc
     5461 tgagcgtgaa cggccgggtc gcggcccgcc atgacggcat gtcgatccgc tggatgagcg
     5521 ataccgcctg gaacaagatc cgcaccccgg cgctcgccgc cagtgccgcg cagcatgacc
     5581 cgctggccca gtcaccggcg ccgctggcac cgttccaggt cggccggcac tggctgagca
     5641 atgtcgtgct cgggcgcgag atcgtcgtgg aggacggcca tctggtcgcc gcgctgatcg
     5701 tcgaccagtc caacccggcg atcttcgatc acccgctgga ccatatcccc ggcatgctcc
     5761 tgctcgaagg cttccgccag ctcgccctgg tcggcgccaa gcggcaactg ggcctggccc
     5821 ccgacgaact gctgctcggc cgggccaggg tggcgttcac ccgcttcggc gagttcggcc
     5881 tggcgaccca ggtcaaagcc aacctggaca gcctgcgcgt gctggacgat ggccgggctg
     5941 tggtgaacct gtcggtggag cagggcgggg cgcagctggc gcatgccgag gtggaattgc
     6001 agcgtctgtt ccagccactg gcgctggccg ccgcgggagg tgcctgatga gtgccatcga
     6061 gcgggtctgg ttcgatttcg gcggtgtgct gtcgccgccc atcgacgcgt tgttcgcgca
     6121 gtacgaagtg aagaccggtc tggcccccgc ggtgttgcag cgggccttcg ccgaggtcgc
     6181 cgaggcgctc ggcgtgccgc cgctggcacc gatcgaaaac gcgctgctca gcgaagacga
     6241 gtgggggcgc cgtgtcgagc tggcgttgag tcgccaggag ccgccggcgg acctgtcgcg
     6301 ggcgcgcctg cggcgcttcg gcgagcagtg gttcgccggc gtgcaggcca acgccgcgat
     6361 ggtcgcggcg gcgcgcaagc tcaaggagtc cgggttcgcc atcggcatcc tgaccaacaa
     6421 cgtggtcgag tgggaaccct actggcgcgc catcgtcggc ctcgacgaag tcgccgacct
     6481 gatcgtcgac tcgtcgaagg aaggctgccg caaacccgat gcgcagttct tcgacatcgc
     6541 ccggcagcgc gccgggctgc ccgccgcggc caatctgctg atcgatgacc tggccgagaa
     6601 tgtcgccgct gccgctgccc tgggctggcg cactgtgcat tttcgcgaca accgccaggc
     6661 cctgggcgac atcacccacg ccaccggtgt ctgcgttttt tccgtgcagc agcctgaagg
     6721 agcacctgcc tgatgaacat cgccctcgac agcctgcccg gcgccgccgc ccggctcgcg
     6781 gatatcgaca tcacccacat cgccaacctg gatgccgcgc cggcctaccg gccgctgccc
     6841 gccgaaggcg gtttccagcg catcctgctg cccagcgggc acagcgccat ccacctgacc
     6901 cgctatgccg aggtgcaggc gctgctgctc gacaaccgcg ccatccgcgc gccgtgcaac
     6961 gaggaggatg ggccgagttt cctgcccacc atcaccccgc cggagctgct gctgaacaac
     7021 gacatccccg accatggccg gctgcgcaag gtggtggcgc gggacttcag tcccagcggc
     7081 gtcgcggtgt tgcgcgaggc ggtgcggcag gtcacccatg cgcgcatcga cctgctgcgc
     7141 cagcaggggc gcggcgccga cctgttcagc ctggtgctgg agcaggtgcc gtccaccgtg
     7201 gactgccagc tgctcggcat tcccctggcc gaccgcgact actaccggcc gctgagccac
     7261 accgtgcaga tcgcctcgga ccacgatgtg cctgacctgc tgcggcagtt ctggggcgtc
     7321 tacgactacc tcaccgacct ggtcaccggc gcccgtccca gcctgccgga cggtttgatc
     7381 cagcgtttca tcgccgcgcg caacgacagc gagccgccgc tgagcgacaa ggaactggtg
     7441 gggattctcc tcggtgtgct gatcggcggt gaccagaaca tcctcagcgt gctgaccaag
     7501 gcggtctatg tcctgctcgc gcaaccggtg ctgtaccgca gcctggtgga cgaccccggg
     7561 ctgattccgt cggcggtgga ggaactgttg cgcctgctgc cgctgggcac catttcgacc
     7621 ttcccgcgcc tggccagcag cgatatcgaa gggccctggg ggctgatccc ggcgggcagc
     7681 gtgatctacg ccgatgcgtt cgccgccaac cgcgatcccg aggtgttcag cgatccgctg
     7741 gctatccgcc tcgaccgcca ggaggcccgg cacctgcagt tcggctacgg catgcacaac
     7801 tgcatgggcg ccgcgctggt gcgcctggag atcgccacgg tgctggagat tctcgtcgag
     7861 cgcctgcccg acctgcgcct ggacatcgcg gcggagcagg tgccgtgggt cgagggcatc
     7921 atcctgcgcc gtccgacccg cctgccggta cgcggcttca cctctctctg aacctctgga
     7981 cggccccatg aacagcaagc tttcggacaa cgccctgctg ggcaccattc tcctggcgat
     8041 ctttgtcgtc cccagttcca tctccggcac cgcgctggcc ctgccggcga tcggcaatga
     8101 cattcaggcc tcgctggccc agctgcagtg ggtggtcaac gcgttcaacc tgaccttcgc
     8161 ctgcttcacc ctggcctggg gggcgctggc tgatcgcctg ggccgcaagc gctgcttcgt
     8221 ggtcggcgcc ggcctgtatg tgctggcttc ggtcatctcg gccctggcca gccaggcctg
     8281 ggtactggat gccgggcggg cgctggccgg gattggcgcg gcggcgatct tctcctgcgg
     8341 catcgccatt ctgtccacgc acttcgacgg cgcggcgcgg ttgcgcgcct tcgccctgtt
     8401 cggcacggtc gccggccttg gcgtcagcct cggcccgacc ctgtccggcc tgctgatcga
     8461 acacttcggc tggcgggcga tcttctgggc tcatctgctg acccttggcc tggtgctgct
     8521 gggcacgccg ttgatcaccc gcgacggcca actggccgaa cgcacggcgc gtttcgacct
     8581 gctcggttcg ttgctgttca tcctcgccct gctggccttg atggtggcca tcgtccaggg
     8641 ctcgcagtgg ggctggcgca gccagggcgt gctggggctg gtggcgctgt cggcggtgct
     8701 gctggtgctg ttcgcctggc aggaacgtcg tcaccggcaa ccgatgctcg acctgtcgct
     8761 gctgaccagc gggcctttcg tcgggctggc gctggttacg gtggcggcgt cgttcggctt
     8821 cgtgaccctg ctgacctacc tgccgagcta tctgttcggg gtcttgcgcc tggcgccgac
     8881 ccaggccggg ctggccatgc tgctgcttac cgtgccgatg ctgttctgcc cgattctcgc
     8941 gggtaagtgg gcggcccggg gcgtttccgc ggtggccatc ctcaaggcca gcctgctggc
     9001 cttgctggcc ggggtggtcg gcctggcgct gctggcccag gcccatgcgg cgctctggca
     9061 tctcgccgca ccgctgttgc tggtgggcgt gggcatgggg ctgtccgccg gcctggtgga
     9121 tggcctggcg ctgaagaccg tgccggagca gaaggccggc atggccgccg gcctgctcaa
     9181 taccttccgc ctgggcagcg aggccattgc cgtggcggtg tacggctcgt tgctggcgac
     9241 tctgctcgat gaccagttgc gtcaggaact ggcccgcctc agcagcgacc cggccaccgt
     9301 ccgcggctgg atcgacagcg tcgccgccgg caacctcgaa ggcgcgctgc gcggtgtggc
     9361 gcagcccgag ctggtcggtg atctgctgcg cggcggttac gacgcggcgt tccacggcgt
     9421 gctgtggttc ctggcggcgg tgatcgtggt tctgatcctg gtcatcggcg ccttgctgcg
     9481 gccactgcgc cagcaggaaa ggggggcgct ggcggtgcag tgagcacggc cgccacgggt
     9541 ctttggccag ggtggcgcag ccggtctgcc gcgggcatcc ggcggcgctg gcgcgccatt
     9601 ctggtcaagg atggatatgc attaattgaa tgtttgtaac gttttttatt atttttcagg
     9661 atgattgccc tgatgcagac tcgactcaac tgccagggaa gcagtcaacc gatcacctga
     9721 ccggccacca ccttcttgtc cgatggcgtg tgcagtcccg cagaagattg tcagaccatg
     9781 aatggacaaa acctgctcaa gcagcagacc gtgacccgca tcttccagca gcattatctt
     9841 tggctgtgct cacgcctggc gtttcgcacc ggttgcaacc acagcgccga ggacatcgcc
     9901 gcggaaacct tcctgcgtgt ctggatgctc cccgatccga cggcgatccg cgagccacgg
     9961 gcgttgctga cgaccatcgc gcagcgcctg atgtacgaga cctggcgccg ccgcgacctg
    10021 gaaaaagcct acctgcagtc actcagcctg gtccccgagg aactgcaccc ctcgccccag
    10081 gagcaactga tgctgatcca gagcctggtg gccatcgacc gcctgctgga aggcctgtcc
    10141 cgccaggccc gcagcgtctt cgtcctcagt cagctggagg gcctgaccta cgtgcagatc
    10201 cagcagcgcc tgggtttgtc cctcgggcgt atccaccagc tcatggccca ggccctgcgc
    10261 tgctgctacc tggggctgga agaatgaacc cgcagcgcct cgacgatccg gtggcggagg
    10321 aggccatcga gtggatggtg cgcctgcagt cgggggcctt cgatgacggc cagcaggctg
    10381 aactggagca ttggcgcaac cgctccagcc atcaccgcca ggtgtatcag cgcctgctgg
    10441 cggggctggc gccgtttcgc cagtcgccgt ggcgcggtcg cgccagccag ccgttgctga
    10501 gcagcctcga acaacccgat ggccgccggg cgttcctgcg aacggcgctg ggcctcagtc
    10561 tgctggtcgg tggcggcgcc tggctcggcg gggtgcaagg gtttgccggc ggcgccgcgc
    10621 aggagtaccg caccgccacc gccgagcgtg gcagctggcg cctgagcgat ggcagccagt
    10681 tgcagctcaa tgcgcgcagt cgggtgtcgt tgtggttcga tggcgaacag cggcgcctgc
    10741 gcctgcacga aggggaaatg ctgctgcagg tggcgccgct ggccagtcgg ccgctggcga
    10801 tcgagaccgc ttccggcacc ctgtacagcc acggtgggca actggcgctg cgcgaactgg
    10861 cggaccgttc gcggctggtc agcctgcgcg aagacattcg cctgcagccc tccggcgcag
    10921 cggcgctgtc cctgcgcgcc gggcgggtga cctggttcga cgcccgcggg gtactcgaac
    10981 aggccccgat gggctccatg gaaaccgcct ggctgcaagg ctggctggtg gtgcacaacg
    11041 aaccgct
//
