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Overview
1.1
2.1
2.2

### Identified secondary metabolite regions using strictness 'relaxed'

NZ_HE998567.1

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | NRPS | 955,360 | 1,001,743 |

NZ_HE998568.1

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 2.1 | RRE-containing | 64,328 | 84,606 |
Region 2.2 | cyclic-lactone-autoinducer | 353,455 | 374,012 |

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 1.1 | NRPS | 955,360 | 1,001,743 |
Region 2.1 | RRE-containing | 64,328 | 84,606 |
Region 2.2 | cyclic-lactone-autoinducer | 353,455 | 374,012 |

No secondary metabolite regions were found in these records:
  NZ_HE998569.1
  NZ_HE998570.1
  NZ_HE998571.1
  NZ_HE998572.1
  NZ_HE998573.1
  NZ_HE998574.1
  NZ_HE998575.1
  NZ_HE998576.1
  NZ_HE998577.1
  NZ_HE998578.1
  NZ_HE998579.1
  NZ_HE998580.1
  NZ_HE998581.1

Compact view
NZ_HE998567.1 - Region 1 - NRPS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 955,360 - 1,001,743 nt. (total: 46,384 nt) Show pHMM detection rules used
NRPS: cds(Condensation and (AMP-binding or A-OX))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
NRPS/PKS domains
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
Pfam domains
TIGRFAM domains
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | NRPS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001343.1 |  | 0.27 | Polyketide | aurachin A, aurachin D | Stigmatella aurantiaca Sg a15
BGC0001585.1 |  | 0.26 | Alkaloid | melinacidin IV | Escovopsis weberi
BGC0000321.1 |  | 0.26 | NRP | chaetocin | Collariella virescens
BGC0000417.1 |  | 0.25 | NRP | rhodochelin | Rhodococcus jostii RHA1
BGC0001123.1 |  | 0.25 | NRP | aspirochlorine | Aspergillus oryzae RIB40
BGC0001771.1 |  | 0.25 | NRP | scleric acid | Streptomyces sclerotialus
BGC0000343.1 |  | 0.24 | NRP | enterobactin | Pseudomonas sp. J465
BGC0001185.1 |  | 0.24 | NRP | bacillibactin | Bacillus velezensis FZB42
BGC0001149.1 |  | 0.23 | NRP, Saccharide | brabantamide A | Pseudomonas sp. SHC52
BGC0001833.1 |  | 0.23 | NRP | icosalide A, icosalide B | Burkholderia gladioli

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001343.1 |  | 0.61 | Polyketide | aurachin A, aurachin D | Stigmatella aurantiaca Sg a15
BGC0001585.1 |  | 0.60 | Alkaloid | melinacidin IV | Escovopsis weberi
BGC0000321.1 |  | 0.60 | NRP | chaetocin | Collariella virescens
BGC0000417.1 |  | 0.59 | NRP | rhodochelin | Rhodococcus jostii RHA1
BGC0001123.1 |  | 0.58 | NRP | aspirochlorine | Aspergillus oryzae RIB40
BGC0001771.1 |  | 0.58 | NRP | scleric acid | Streptomyces sclerotialus
BGC0000343.1 |  | 0.57 | NRP | enterobactin | Pseudomonas sp. J465
BGC0001185.1 |  | 0.57 | NRP | bacillibactin | Bacillus velezensis FZB42
BGC0001149.1 |  | 0.56 | NRP, Saccharide | brabantamide A | Pseudomonas sp. SHC52
BGC0001833.1 |  | 0.56 | NRP | icosalide A, icosalide B | Burkholderia gladioli

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Module view
Shows module structures for each candidate in NRPS- and PKS regions. Genes are shown in predicted order, and are only present when containing at least one complete module. A domain glossary is available here.
Legend
Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names
NRPS/PKS products
NRPS/PKS monomers
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 955359 - 1001743:
Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:
  (X - pro)

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS monomer predictions
Shows the predicted monomers for each adynelation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).

ctg1_921: X - pro
  Search NORINE for peptide: strict or relaxed
  AMP-binding (469..852): X
  NRPSPredictor2: hydrophobic-aromatic
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aromatic
    Large clusters prediction:
      N/A
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DAisLGqIgK
    Nearest Stachelhaus code:
      bht
    Stachelhaus code match:
      60% (weak)
  AMP-binding (1485..1863): pro
  NRPSPredictor2: pro
  SVM prediction details:
    Predicted physicochemical class:
      hydrophobic-aliphatic
    Large clusters prediction:
      N/A
    Small clusters prediction:
      pro
    Single AA prediction:
      pro
  Stachelhaus prediction details:
    Stachelhaus sequence:
      DvTqIvcVyK
    Nearest Stachelhaus code:
      asn
    Stachelhaus code match:
      50% (weak)
ctg1_925: X
  Search NORINE for peptide: strict or relaxed
  AMP-binding (30..445): X
  NRPSPredictor2: (unknown)
  SVM prediction details:
    NOTE: outside applicability domain
    Predicted physicochemical class:
      hydrophilic
    Large clusters prediction:
      N/A
    Small clusters prediction:
      N/A
    Single AA prediction:
      N/A
  Stachelhaus prediction details:
    Stachelhaus sequence:
      gLcrvGGPLK
    Nearest Stachelhaus code:
      ala
    Stachelhaus code match:
      60% (weak)

NZ_HE998568.1 - Region 1 - RRE-containing
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 64,328 - 84,606 nt. (total: 20,279 nt) Show pHMM detection rules used
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
MIBiG comparison
KnownClusterBlast
Pfam domains
TIGRFAM domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | RRE-containing | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000938.1 |  | 0.16 | Other (Aminocoumarin) | fosfomycin | Streptomyces fradiae
BGC0000554.1 |  | 0.16 | RiPP | SRO15-3108 | Streptomyces filamentosus NRRL 15998
BGC0000487.1 |  | 0.16 | RiPP | carnocyclin | Carnobacterium maltaromaticum
BGC0001210.1 |  | 0.16 | RiPP | pseudomycoicidin | Bacillus pseudomycoides DSM 12442
BGC0000551.1 |  | 0.16 | RiPP | SapB | Streptomyces coelicolor A3(2)
BGC0000602.1 |  | 0.16 | RiPP | subtilosin A | Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001526.1 |  | 0.16 | Other | bartolosides E, bartolosides F, bartolosides G, bartolosides H, bartolosides I, bartolosides J, bartolosides K | Synechocystis salina LEGE 06099
BGC0000626.1 |  | 0.16 | RiPP | thuricin | Bacillus thuringiensis
BGC0000539.1 |  | 0.16 | RiPP | nukacin ISK-1 | Staphylococcus warneri
BGC0000501.1 |  | 0.15 | RiPP | catenulipeptin | Catenulispora acidiphila DSM 44928

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000938.1 |  | 0.47 | Other (Aminocoumarin) | fosfomycin | Streptomyces fradiae
BGC0000554.1 |  | 0.47 | RiPP | SRO15-3108 | Streptomyces filamentosus NRRL 15998
BGC0000487.1 |  | 0.46 | RiPP | carnocyclin | Carnobacterium maltaromaticum
BGC0001210.1 |  | 0.46 | RiPP | pseudomycoicidin | Bacillus pseudomycoides DSM 12442
BGC0000551.1 |  | 0.46 | RiPP | SapB | Streptomyces coelicolor A3(2)
BGC0000602.1 |  | 0.46 | RiPP | subtilosin A | Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0001526.1 |  | 0.46 | Other | bartolosides E, bartolosides F, bartolosides G, bartolosides H, bartolosides I, bartolosides J, bartolosides K | Synechocystis salina LEGE 06099
BGC0000626.1 |  | 0.46 | RiPP | thuricin | Bacillus thuringiensis
BGC0000539.1 |  | 0.46 | RiPP | nukacin ISK-1 | Staphylococcus warneri
BGC0000501.1 |  | 0.46 | RiPP | catenulipeptin | Catenulispora acidiphila DSM 44928

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names
NZ_HE998568.1 - Region 2 - cyclic-lactone-autoinducer
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 353,455 - 374,012 nt. (total: 20,558 nt) Show pHMM detection rules used
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
MIBiG comparison
KnownClusterBlast
Pfam domains
TIGRFAM domains
Similar gene clusters
Shows careas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | cyclic-lactone-autoinducer | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001314.1 |  | 0.17 | Terpene | tirucalla | Arabidopsis thaliana
BGC0001484.1 |  | 0.13 | Other | 2'-chloropentostatin, 2'-amino-2'-deoxyadenosine | Actinomadura sp. ATCC 39365
BGC0000486.1 |  | 0.09 | RiPP | butyrivibriocin AR10 | Butyrivibrio fibrisolvens

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0001314.1 |  | 0.48 | Terpene | tirucalla | Arabidopsis thaliana
BGC0001484.1 |  | 0.41 | Other | 2'-chloropentostatin, 2'-amino-2'-deoxyadenosine | Actinomadura sp. ATCC 39365
BGC0000486.1 |  | 0.33 | RiPP | butyrivibriocin AR10 | Butyrivibrio fibrisolvens

Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.

No matches found.

Detailed Pfam domain annotation
Shows Pfam domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description. Domains with a bold border have Gene Ontology information.
Selected features only
Expand to show all names
Detailed TIGRFAM domain annotation
Shows TIGRFAM domains found in each gene within the region. Click on each domain for more information about the domain's accession, location, and description.
Selected features only
Expand to show all names