GCF_001717125.1_ASM171712v1_genomic - 6 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_MCGI01000001 (original name was: NZ_MCGI01000001.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	ranthipeptide,cyclic-lactone-autoinducer	1,587,775	1,609,847

NZ_MCGI01000003 (original name was: NZ_MCGI01000003.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS	314,153	361,535	TP-1161	RiPP:Thiopeptide	16%
Region 3.2	cyclic-lactone-autoinducer	602,415	621,834
Region 3.3	cyclic-lactone-autoinducer	642,515	663,084

NZ_MCGI01000004 (original name was: NZ_MCGI01000004.1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	lanthipeptide-class-ii	477,721	500,603
Region 4.2	ranthipeptide	739,219	760,616

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	ranthipeptide,cyclic-lactone-autoinducer	1,587,775	1,609,847
Region 3.1	NRPS	314,153	361,535	TP-1161	RiPP:Thiopeptide	16%
Region 3.2	cyclic-lactone-autoinducer	602,415	621,834
Region 3.3	cyclic-lactone-autoinducer	642,515	663,084
Region 4.1	lanthipeptide-class-ii	477,721	500,603
Region 4.2	ranthipeptide	739,219	760,616

No secondary metabolite regions were found in these records:: NZ_MCGI01000002 (original name was: NZ_MCGI01000002.1); NZ_MCGI01000005 (original name was: NZ_MCGI01000005.1); NZ_MCGI01000006 (original name was: NZ_MCGI01000006.1); NZ_MCGI01000007 (original name was: NZ_MCGI01000007.1); NZ_MCGI01000008 (original name was: NZ_MCGI01000008.1); NZ_MCGI01000009 (original name was: NZ_MCGI01000009.1); NZ_MCGI01000010 (original name was: NZ_MCGI01000010.1); NZ_MCGI01000011 (original name was: NZ_MCGI01000011.1)

Compact view

c00001_NZ_MCGI.. - Region 1 - cyclic-lactone-autoinducer,ranthipeptide

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Location: 1,587,775 - 1,609,847 nt. (total: 22,073 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or YcaO or TIGR03882)))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001929.1	0.54	RiPP	WGK	Streptococcus ferus
BGC0001550.1	0.48	RiPP	citrulassin D	Streptomyces katrae
BGC0001551.1	0.47	RiPP	citrulassin E	Streptomyces glaucescens
BGC0001496.1	0.47	RiPP	actinokineosin	Actinokineospora spheciospongiae
BGC0001634.1	0.47	RiPP	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001552.1	0.47	RiPP	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000575.1	0.46	RiPP	lariatin	Rhodococcus jostii
BGC0001291.1	0.46	RiPP	enterocin NKR-5-3B	Enterococcus faecium
BGC0001549.1	0.45	RiPP	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001645.1	0.44	RiPP	lagmysin	Streptomyces sp. NRRL S-118

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001929.1	0.61	RiPP	WGK	Streptococcus ferus
BGC0001550.1	0.57	RiPP	citrulassin D	Streptomyces katrae
BGC0001551.1	0.57	RiPP	citrulassin E	Streptomyces glaucescens
BGC0001496.1	0.57	RiPP	actinokineosin	Actinokineospora spheciospongiae
BGC0001634.1	0.57	RiPP	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001552.1	0.57	RiPP	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000575.1	0.56	RiPP	lariatin	Rhodococcus jostii
BGC0001291.1	0.56	RiPP	enterocin NKR-5-3B	Enterococcus faecium
BGC0001549.1	0.56	RiPP	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001645.1	0.55	RiPP	lagmysin	Streptomyces sp. NRRL S-118

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

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Detailed TIGRFAM domain annotation

Selected features only

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c00003_NZ_MCGI.. - Region 1 - NRPS

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Location: 314,153 - 361,535 nt. (total: 47,383 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Pfam domains

TIGRFAM domains

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000313.1	0.25	NRP	beauvericin	Beauveria bassiana
BGC0001686.1	0.25	NRP	N-octanoyl-Met-Phe-H	Clostridium sp. CAG:567
BGC0001185.1	0.24	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000308.1	0.24	NRP	aureusimine	Staphylococcus aureus subsp. aureus str. JKD6008
BGC0001132.1	0.23	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.23	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.1	0.23	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.1	0.23	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000401.1	0.23	NRP	paenibactin	Paenibacillus elgii B69
BGC0001135.1	0.22	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000313.1	0.59	NRP	beauvericin	Beauveria bassiana
BGC0001686.1	0.58	NRP	N-octanoyl-Met-Phe-H	Clostridium sp. CAG:567
BGC0001185.1	0.57	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0000308.1	0.57	NRP	aureusimine	Staphylococcus aureus subsp. aureus str. JKD6008
BGC0001132.1	0.56	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.56	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.1	0.56	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.1	0.56	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000401.1	0.56	NRP	paenibactin	Paenibacillus elgii B69
BGC0001135.1	0.55	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known gene clusters

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Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 314152 - 361535:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (tyr - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg3_290

Search NORINE for peptide: strict or relaxed

AMP-binding (24..448): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: tycliGGPLK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 50% (weak)

ctg3_293

Search NORINE for peptide: strict or relaxed

AMP-binding (461..852): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGqVaK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 80% (moderate)

AMP-binding (1492..1881): X

NRPSPredictor2: phe, trp, phg, tyr, bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DivqIGmVCK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 60% (weak)

c00003_NZ_MCGI.. - Region 2 - cyclic-lactone-autoinducer

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Location: 602,415 - 621,834 nt. (total: 19,420 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000486.1	0.09	RiPP	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000526.1	0.05	RiPP	macedocin	Streptococcus macedonicus
BGC0001067.1	0.05	Polyketide, Terpene	fumagillin, β-trans-bergamotene, fumagillol	Aspergillus fumigatus Af293
BGC0001133.1	0.05	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001902.1	0.03	NRP, Polyketide	bengamide	Myxococcus virescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000486.1	0.32	RiPP	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000526.1	0.23	RiPP	macedocin	Streptococcus macedonicus
BGC0001067.1	0.23	Polyketide, Terpene	fumagillin, β-trans-bergamotene, fumagillol	Aspergillus fumigatus Af293
BGC0001133.1	0.22	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001902.1	0.16	NRP, Polyketide	bengamide	Myxococcus virescens

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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c00003_NZ_MCGI.. - Region 3 - cyclic-lactone-autoinducer

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Location: 642,515 - 663,084 nt. (total: 20,570 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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transport-related genes

regulatory genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001521.1	0.16	NRP	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000486.1	0.09	RiPP	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000526.1	0.03	RiPP	macedocin	Streptococcus macedonicus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001521.1	0.47	NRP	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000486.1	0.33	RiPP	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000526.1	0.16	RiPP	macedocin	Streptococcus macedonicus

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

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c00004_NZ_MCGI.. - Region 1 - lanthipeptide-class-ii

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Location: 477,721 - 500,603 nt. (total: 22,883 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Lanthipeptides

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000517.1	0.13	RiPP	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000548.1	0.13	RiPP	salivaricin A	Streptococcus salivarius
BGC0000502.1	0.13	RiPP	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001339.1	0.03	Polyketide	squalestatin S1	Phoma sp. MF5453

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000517.1	0.42	RiPP	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000548.1	0.41	RiPP	salivaricin A	Streptococcus salivarius
BGC0000502.1	0.41	RiPP	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001339.1	0.15	Polyketide	squalestatin S1	Phoma sp. MF5453

Similar known gene clusters

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg4_436 - Putative Class II

ctg4_437 leader / core peptide: MGNEREQFMENAG - NVLFEVEEDhaGKEMMNDhaNQIPANDhbEDhaDhbPFLDhaIFCC
allorf_0481227_0481298 leader / core peptide: LGIQKIRAAITGC - FFDhaDhaVPACPL

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed Pfam domain annotation

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Detailed TIGRFAM domain annotation

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Lanthipeptides

Core peptide predictions

ctg4_437 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 3699.7 Da

Molecular weight: 3702.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3720.2 Da N = 1; 3738.2 Da N = 2; 3756.2 Da N = 3; 3774.2 Da N = 4

allorf_0481227_0481298 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 1030.5 Da

Molecular weight: 1031.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1049.2 Da N = 1

c00004_NZ_MCGI.. - Region 2 - ranthipeptide

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Location: 739,219 - 760,616 nt. (total: 21,398 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or YcaO or TIGR03882)))

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core biosynthetic genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	ranthipeptide	Similarity score	Type	Compound(s)	Organism
BGC0000344.1		0.04	NRP	entolysin	Pseudomonas entomophila L48

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000344.1		0.21	NRP	entolysin	Pseudomonas entomophila L48

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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