LOCUS       NZ_CP035998.1          12313 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_CP035998.1 Bacillus mycoides strain BPN36/3 plasmid p481.
ACCESSION   NZ_CP035998
VERSION     NZ_CP035998.1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 13:07:22
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 34413
            Orig. end    :: 46726
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12313
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[39413:41726](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7313
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..12313
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193)"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12313
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193)"
                     /tool="antismash"
     CDS             complement(281..808)
                     /locus_tag="ctg2_30"
                     /transl_table=11
                     /translation="MNFRKGITAFLLGSVFVFSSVGVSEAYVKEGWKLPSKSATYKWGD
                     RLDDGSSIIKSGWQAADSAWYTASRINFTVSSSSVNKLNSWFESSSTYYGRMNTTYNTS
                     TKKVTKFAGDINAGNTNITKSNVAKSTGVHEFGHAIGIGHNSGTSIMNSNRNRTTMHVP
                     QTDDKNGVNAIY"
     PFAM_domain     complement(284..442)
                     /aSDomain="Peptidase_M10"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00413.26"
                     /description="Matrixin"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_30_0001"
                     /evalue="5.50E-07"
                     /label="ctg2_30"
                     /locus_tag="ctg2_30"
                     /protein_end="175"
                     /protein_start="122"
                     /score="29.6"
                     /tool="antismash"
                     /translation="TKSNVAKSTGVHEFGHAIGIGHNSGTSIMNSNRNRTTMHVPQTDD
                     KNGVNAIY"
     CDS             945..1718
                     /locus_tag="ctg2_31"
                     /transl_table=11
                     /translation="MYKHKVIKWSLCLLIFTFLIIIGKTIWSNTDIAEMSNVSPNSETI
                     VLSKTFKTTNHLSEMVEEADVIVLGEYYGLHSKWNMANNSSHEIPGEEVEGHLFNFHVK
                     EILKGDSVENKILINHRYSETLVLEESNEVIDDNGIILKGATKVCTKKVKNKDPLYIKP
                     KSGDTYIVFLKENSKLGHFYPALEPFMIQFDLVNIAHLKSNLINWDKNKYKFETKVNEK
                     TFYIENEIDSTIKDNISGKTLKEIKNFVKKTSKMN"
     CDS             2082..2489
                     /locus_tag="ctg2_32"
                     /transl_table=11
                     /translation="MIFQSMITAIIEKLQETVNPYWIIVFGSTAEDRERDDSDIDIAYL
                     SDIKLGNYERFLLAQELADIVHRDVDLVDLSEASTVFQAQIIHTGKTIFCSDEERKTIF
                     EIKTLKMYSKLNEERKIVFEDIKKRGSIYEE"
     PFAM_domain     2109..2378
                     /aSDomain="Polbeta"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF18765.3"
                     /description="Polymerase beta, Nucleotidyltransferase"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_32_0002"
                     /evalue="1.50E-22"
                     /label="ctg2_32"
                     /locus_tag="ctg2_32"
                     /protein_end="99"
                     /protein_start="9"
                     /score="79.7"
                     /tool="antismash"
                     /translation="IIEKLQETVNPYWIIVFGSTAEDRERDDSDIDIAYLSDIKLGNYE
                     RFLLAQELADIVHRDVDLVDLSEASTVFQAQIIHTGKTIFCSDEE"
     CDS             2479..2892
                     /locus_tag="ctg2_33"
                     /transl_table=11
                     /translation="MKNEVILNKISTIERCIKRIQEVYGNNPDNLEDYTKQDSIILNIQ
                     RACEASIDLAMHIVAGKKLGLPQSSREAFDLLVDADLLSTELASKLKAMVGFRNIAVHD
                     YQSVNLDIVRQIIEKHLKDFKLFTKEVMGILDL"
     PFAM_domain     2515..2868
                     /aSDomain="DUF86"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF01934.19"
                     /description="Protein of unknown function DUF86"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_33_0003"
                     /evalue="1.80E-26"
                     /label="ctg2_33"
                     /locus_tag="ctg2_33"
                     /protein_end="130"
                     /protein_start="12"
                     /score="92.5"
                     /tool="antismash"
                     /translation="IERCIKRIQEVYGNNPDNLEDYTKQDSIILNIQRACEASIDLAMH
                     IVAGKKLGLPQSSREAFDLLVDADLLSTELASKLKAMVGFRNIAVHDYQSVNLDIVRQI
                     IEKHLKDFKLFTKE"
     CDS             3130..3345
                     /locus_tag="ctg2_34"
                     /transl_table=11
                     /translation="MQIRENNNSNEILIAAAVSAIEQMKYEIAQEFGVTLGPDSTARAN
                     GSVGGEITKRLVRMAQEQLSGQYKIH"
     PFAM_domain     3157..3321
                     /aSDomain="SASP"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00269.22"
                     /description="Small, acid-soluble spore proteins,
                     alpha/beta type"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_34_0004"
                     /evalue="5.10E-19"
                     /label="ctg2_34"
                     /locus_tag="ctg2_34"
                     /protein_end="64"
                     /protein_start="9"
                     /score="68.2"
                     /tool="antismash"
                     /translation="NEILIAAAVSAIEQMKYEIAQEFGVTLGPDSTARANGSVGGEITK
                     RLVRMAQEQL"
     CDS             complement(3418..3831)
                     /locus_tag="ctg2_35"
                     /transl_table=11
                     /translation="MKKVILAGALGIAVLSGMNLPGAEAPKASAATTNLEIQEVTKYYS
                     MSSYEVSQKETFNLNKGEELTVFVQNSGFPISYTVFDADNQVIGTYNANSPYGRVFKVQ
                     KDGNISVQFQAGANSSYMKKMNFTAKIAISKLN"
     CDS             complement(4155..4646)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg2_36"
                     /sec_met_domain="Abi (E-value: 1.4e-11, bitscore: 39.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRNIHNFLSNLNKIPFILLMTIISFLITIPLNLFLPPSQANPSI
                     NESIIEHILLVLIIAPFLETLLFQKVLFFILQLSSYISERNILTILIASIIFGLTHQFD
                     ITYIAAATLMGIVFNYSYSIYQEKNYNDPKSMSAFWIVFWIHELHNTIAFLIIEFGQKL
                     "
     PFAM_domain     complement(4194..4493)
                     /aSDomain="Rce1-like"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF02517.18"
                     /description="Type II CAAX prenyl endopeptidase Rce1-like"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_36_0005"
                     /evalue="6.70E-11"
                     /label="ctg2_36"
                     /locus_tag="ctg2_36"
                     /protein_end="151"
                     /protein_start="51"
                     /score="42.5"
                     /tool="antismash"
                     /translation="HILLVLIIAPFLETLLFQKVLFFILQLSSYISERNILTILIASII
                     FGLTHQFDITYIAAATLMGIVFNYSYSIYQEKNYNDPKSMSAFWIVFWIHELHNT"
     CDS             complement(4729..4929)
                     /locus_tag="ctg2_37"
                     /transl_table=11
                     /translation="MNEKLSTNFQELTEKELQTNGGAIRILSKVYEAANHCLRDNYCRG
                     RLGNDMGNAGRIEAGHYGGRP"
     CDS             5001..7313
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 297.3; E-value:
                     3.8e-90)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_38"
                     /sec_met_domain="TIGR01193 (E-value: 2.4e-212, bitscore:
                     701.9, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.3e-43, bitscore:
                     141.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYMQNCIFSLFSLYSDIIKTDIKIQGKEAAILFTKKISYIRQQDL
                     KDCGPACLAMISRYHGFTMSISKIREIAGTDLEGTNIKGLIEAGEKLGFEVKGVKATNF
                     EAFKEIPLPAIAHVVMDGGLLHYVVIHKVKNNKIYIVDPEKGFATYSLEEFGQIWTGIL
                     VLMTPSGDFQTGKSSQSTLSRFIFLLRQQTNLLVPLFFASIFFNLFGLLGAFYFKFLID
                     DIVTNQLLQTLHIVSIGIVILYIFKVFLSYFRTHLILYLSRRIDIQLMLGYYRHVVGLP
                     MNFFETRKVGEIISRFMDASKIRDALSTITVTLMIDTVMVTLGAVLLYIHSPVLFGVTL
                     LLIPFYIGIIFAFRKPYQHINREEMESNAKLNSYLVESLNGIATIKSYNAEKEVFFQTE
                     NRFVNLLRYVFKRGMLSNLQGSIKMGLELIGGTVILWVGAMQVLKGSMTIGELITYNAL
                     LGYFLNPIENLIGIQPTMQSALVAGERLNEIFDLDAEKDEKEHKKVSPKQLSGKIECSN
                     VTFRYGTRKNVLNNISFSIEQGSQVAFVGESGSGKTTISKLLMNYYEAQEGVIYYEDYH
                     IKEINRTALRDRIAYVSQESFFFSASIYENLRFGLERQVSLEEMIHVCKLACIHEFISE
                     LPLSYETLLEENASNLSGGQRQRLAIARALLKKPDILILDEATSNLDSTTEKHITDMLK
                     ELGLQGVTVIMIAHRLSTIQHANRIFVMEKGNIIERGSHEELLFHKGEYYRLWKNQTVH
                     VEHIESQSYAMRGGQYE"
     PFAM_domain     5109..5507
                     /aSDomain="Peptidase_C39"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF03412.17"
                     /description="Peptidase C39 family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_38_0006"
                     /evalue="1.90E-41"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="169"
                     /protein_start="36"
                     /score="141.1"
                     /tool="antismash"
                     /translation="ISYIRQQDLKDCGPACLAMISRYHGFTMSISKIREIAGTDLEGTN
                     IKGLIEAGEKLGFEVKGVKATNFEAFKEIPLPAIAHVVMDGGLLHYVVIHKVKNNKIYI
                     VDPEKGFATYSLEEFGQIWTGILVLMTPS"
     aSDomain        5124..7244
                     /aSDomain="TIGR01193"
                     /aSTool="tigrfam"
                     /description="bacteriocin_ABC: ABC-type bacteriocin
                     transporter"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg2_38_0001"
                     /evalue="2.40E-211"
                     /identifier="TIGR01193"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="748"
                     /protein_start="41"
                     /score="701.9"
                     /tool="antismash"
                     /translation="QQDLKDCGPACLAMISRYHGFTMSISKIREIAGTDLEGTNIKGLI
                     EAGEKLGFEVKGVKATNFEAFKEIPLPAIAHVVMDGGLLHYVVIHKVKNNKIYIVDPEK
                     GFATYSLEEFGQIWTGILVLMTPSGDFQTGKSSQSTLSRFIFLLRQQTNLLVPLFFASI
                     FFNLFGLLGAFYFKFLIDDIVTNQLLQTLHIVSIGIVILYIFKVFLSYFRTHLILYLSR
                     RIDIQLMLGYYRHVVGLPMNFFETRKVGEIISRFMDASKIRDALSTITVTLMIDTVMVT
                     LGAVLLYIHSPVLFGVTLLLIPFYIGIIFAFRKPYQHINREEMESNAKLNSYLVESLNG
                     IATIKSYNAEKEVFFQTENRFVNLLRYVFKRGMLSNLQGSIKMGLELIGGTVILWVGAM
                     QVLKGSMTIGELITYNALLGYFLNPIENLIGIQPTMQSALVAGERLNEIFDLDAEKDEK
                     EHKKVSPKQLSGKIECSNVTFRYGTRKNVLNNISFSIEQGSQVAFVGESGSGKTTISKL
                     LMNYYEAQEGVIYYEDYHIKEINRTALRDRIAYVSQESFFFSASIYENLRFGLERQVSL
                     EEMIHVCKLACIHEFISELPLSYETLLEENASNLSGGQRQRLAIARALLKKPDILILDE
                     ATSNLDSTTEKHITDMLKELGLQGVTVIMIAHRLSTIQHANRIFVMEKGNIIERGSHEE
                     LLFHKGEYYRLWK"
     PFAM_domain     5595..6395
                     /aSDomain="ABC_membrane"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00664.25"
                     /description="ABC transporter transmembrane region"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_38_0007"
                     /evalue="8.10E-46"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="465"
                     /protein_start="198"
                     /score="157.0"
                     /tool="antismash"
                     /translation="LFFASIFFNLFGLLGAFYFKFLIDDIVTNQLLQTLHIVSIGIVIL
                     YIFKVFLSYFRTHLILYLSRRIDIQLMLGYYRHVVGLPMNFFETRKVGEIISRFMDASK
                     IRDALSTITVTLMIDTVMVTLGAVLLYIHSPVLFGVTLLLIPFYIGIIFAFRKPYQHIN
                     REEMESNAKLNSYLVESLNGIATIKSYNAEKEVFFQTENRFVNLLRYVFKRGMLSNLQG
                     SIKMGLELIGGTVILWVGAMQVLKGSMTIGELITYNALLGYFLNP"
     PFAM_domain     6585..7037
                     /aSDomain="ABC_tran"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00005.29"
                     /description="ABC transporter"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_38_0008"
                     /evalue="8.60E-35"
                     /label="ctg2_38"
                     /locus_tag="ctg2_38"
                     /protein_end="679"
                     /protein_start="528"
                     /score="120.3"
                     /tool="antismash"
                     /translation="LNNISFSIEQGSQVAFVGESGSGKTTISKLLMNYYEAQEGVIYYE
                     DYHIKEINRTALRDRIAYVSQESFFFSASIYENLRFGLERQVSLEEMIHVCKLACIHEF
                     ISELPLSYETLLEENASNLSGGQRQRLAIARALLKKPDILILDEATS"
     CDS             7306..8619
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 63.7; E-value:
                     2.3e-19)"
                     /gene_kind="transport"
                     /locus_tag="ctg2_39"
                     /transl_table=11
                     /translation="MSKIYSFDQLTDSVELLEKRPPRFITGLLCFLCISLLGFMIWAYV
                     GKVDIVSKGTATIQSKSDISVSRTQIPGVVDTVSVKSGDAVKKGDTLLQLKNQESMDKQ
                     NQMDQIVKHLEGQKEMLEQLKKSIKSHKLYFTDEVDKKIREEYKAYEQGYQSLQNEKEN
                     EINVIGNSKLSNEQDEILQGLIAEKENIQREIKSINKQKDKENILDEQKQVLDDKIQSL
                     ESQQNNIEKRIAQRKGTLDSERKKVDTTKEGKQKQKEYALNQYRESAIVSVNQRIQSLE
                     QEIFIKKQDLSGLHNQNETMIIKAHQDGVVQFPSILQPGDLIDSGQEVVSIIPKEDEKR
                     VKILLSAQEIRGIKKGDKVQYSFKLNKTDKQMGKVTYISAHPIFDKDSKGYVYELEATI
                     EKQVLYELHTGMVGKASVVTGEEQIWRFILRKLDFISN"
     PFAM_domain     7513..7629
                     /aSDomain="Biotin_lipoyl_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13533.8"
                     /description="Biotin-lipoyl like"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_39_0009"
                     /evalue="2.90E-08"
                     /label="ctg2_39"
                     /locus_tag="ctg2_39"
                     /protein_end="108"
                     /protein_start="69"
                     /score="33.5"
                     /tool="antismash"
                     /translation="QIPGVVDTVSVKSGDAVKKGDTLLQLKNQESMDKQNQMD"
     PFAM_domain     8212..8511
                     /aSDomain="HlyD_3"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13437.8"
                     /description="HlyD family secretion protein"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_39_0010"
                     /evalue="7.70E-14"
                     /label="ctg2_39"
                     /locus_tag="ctg2_39"
                     /protein_end="402"
                     /protein_start="302"
                     /score="52.4"
                     /tool="antismash"
                     /translation="IKAHQDGVVQFPSILQPGDLIDSGQEVVSIIPKEDEKRVKILLSA
                     QEIRGIKKGDKVQYSFKLNKTDKQMGKVTYISAHPIFDKDSKGYVYELEATIEKQ"
     PFAM_domain     8778..9062
                     /aSDomain="SnoaL_2"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF12680.9"
                     /description="SnoaL-like domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_40_0011"
                     /evalue="2.10E-06"
                     /label="ctg2_40"
                     /locus_tag="ctg2_40"
                     /protein_end="95"
                     /protein_start="0"
                     /score="28.4"
                     /tool="antismash"
                     /translation="MDAFKTYLSENVSWTEAAGFPYAGTYIGPDEVVKNVHERLGTEWD
                     NYSAKDEIYAFNNNTVIVYGKYSGTYKVTGKSFIADFCHLYEFDENDKVK"
     CDS             8778..9113
                     /locus_tag="ctg2_40"
                     /transl_table=11
                     /translation="MDAFKTYLSENVSWTEAAGFPYAGTYIGPDEVVKNVHERLGTEWD
                     NYSAKDEIYAFNNNTVIVYGKYSGTYKVTGKSFIADFCHLYEFDENDKVKKFIQIVDSA
                     TVNEVLS"
     CDS             complement(10801..11370)
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 82.2; E-value: 1.1e-24)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg2_41"
                     /transl_table=11
                     /translation="MIKLEKENKKMKIIQSATILFSMGGVRNTTVDEIAKKAKVGKGTI
                     YYYYTDKTEILCDCYMRHIQLIRSQLFDLYKEEKDVLLRIQKILHYISLEYHQDPFVST
                     LFQEYKEYHSPEITACFKKSEEEAIKMISNLLEKGYQQGALHKVPLPLTAFMLVRMIFN
                     YEQDYIKDDKSDKEFLQLLKHMLLKN"
     PFAM_domain     complement(11203..11334)
                     /aSDomain="TetR_N"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00440.25"
                     /description="Bacterial regulatory proteins, tetR family"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg2_41_0012"
                     /evalue="1.40E-12"
                     /label="ctg2_41"
                     /locus_tag="ctg2_41"
                     /protein_end="56"
                     /protein_start="12"
                     /score="47.2"
                     /tool="antismash"
                     /translation="IIQSATILFSMGGVRNTTVDEIAKKAKVGKGTIYYYYTDKTEIL"
ORIGIN
        1 ttatttggat tacgtgccat attccactta gagtcaagac ctccatattc accaattaca
       61 actagatcgg cttcattaat catttgattt aaatcacttg tagcagggaa atctttactg
      121 agaatgacag tttcagcatt cttatcttcg ccagcaatat tggagaagtg actattatat
      181 gctgaatatc ctccgaaacc aatcactgca gctaaaggaa ttgcaattac taaatttttt
      241 gcttttttat ttaacattaa gaaatcatct cctttttctg ttaatagatt gcatttacac
      301 cattcttatc gtcagtttga ggaacatgca tagtagtacg attacgattg ctgttcataa
      361 ttgaagtgcc actgttgtga cctattccta ttgcatgacc aaactcatgt actcctgtac
      421 ttttagcaac attagatttt gtaatatttg tattccctgc attaatatca ccagcgaatt
      481 tcgttacttt tttagtggat gtattataag ttgtgttcat tcttccataa taagtagagg
      541 aactttcaaa ccaagagttt aatttgttca cagaagaaga agaaactgta aagttaatac
      601 gagaagcagt gtaccaggct gaatctgcag cttgccaacc ggatttaatg atactggagc
      661 catcgtctaa tctgtctccc cacttatatg tagctgattt tgaaggtagc ttccatcctt
      721 cttttacata agcttctgaa actccaacgc tggaaaagac aaacacacta cctaataaaa
      781 atgcagtaat cccttttcta aaattcataa tatccctcca aagattattt ttaacaagag
      841 aattatagca tcattatctt gttttgaatt aatatttttt aaaaattcag aatatttata
      901 ttgagaaaat ggtataaaat atcttttaag gagacgttat tataatgtat aaacataaag
      961 taataaaatg gagtctatgt ttattaatat ttactttttt aattattatt ggtaaaacaa
     1021 tttggtcaaa tacagacata gcagaaatgt ctaatgtttc accgaactct gaaactatag
     1081 tattaagtaa gacattcaaa actacaaatc atttaagtga aatggttgaa gaagcagatg
     1141 taattgtact aggtgaatac tacggcttac actctaagtg gaatatggca aacaattcat
     1201 cacatgagat tccaggtgaa gaagtcgaag gccatctgtt taattttcat gttaaagaaa
     1261 tcttaaaagg ggactctgta gagaataaaa ttttaataaa tcatagatac tcagaaacac
     1321 ttgtattaga agaaagtaat gaagtgattg atgacaatgg aataattttg aaaggtgcta
     1381 cgaaagtgtg tacaaaaaaa gtgaaaaata aagatccatt gtatataaaa cctaaatctg
     1441 gtgacacata tatagttttt ttaaaagaga atagtaaact tggacatttt tatccagctt
     1501 tagaaccttt tatgattcaa tttgatttag tgaatattgc acacctaaaa agtaatctaa
     1561 taaattggga taaaaacaaa tataaatttg aaacaaaggt taatgagaaa actttttata
     1621 ttgaaaatga aatagattca actatcaaag ataatattag tggaaaaaca ttaaaagaaa
     1681 ttaagaattt tgtgaagaaa acttcaaaaa tgaattaaca ttatgacata ctatatttct
     1741 atatataatt gcggatatgg ttctaacacc tttctggtgt atttaaatga ggaaaatata
     1801 aaggtttata aatctgtgaa gttattggat gttaaataat gatataagat ttatacctat
     1861 aagaacttta aattttccgt ttacagcatt aggtcataat cctatcacta aaaaaggaca
     1921 aattcctgca agaaggggat gcttctaaca aaaaaatcta taattttaaa gtgctttaag
     1981 tgtcaagaag gtttaattta attatgtaat tgtgcagctt taaagattag gtaatggtta
     2041 tgatataata aaattcaagt atactaaggg ggattaatta aatgattttt caaagcatga
     2101 ttacagctat tatagaaaaa ttacaagaga ctgtgaatcc ctattggata attgtatttg
     2161 gatccacagc agaggacaga gaacgagatg atagtgacat tgatatagct tatttaagtg
     2221 acataaaatt agggaattat gaacgttttt tattagcaca ggagttagcg gatattgttc
     2281 atagagatgt tgatttagtt gatttatcag aagcttctac agttttccaa gctcaaatta
     2341 tacatacagg aaagacaata ttttgttcgg atgaagagcg aaaaacaatt tttgaaataa
     2401 aaactttaaa aatgtattca aaattgaatg aggaacgcaa aatcgtattt gaggatataa
     2461 agaaaagagg ttctatatat gaagaatgaa gtcattttga ataaaatttc tacaatagag
     2521 cgttgtataa aaagaattca agaggtatat ggaaataacc ctgataattt agaggattat
     2581 acaaagcaag attctataat attaaatatt caaagagcat gcgaagcaag tattgattta
     2641 gcaatgcata ttgttgcagg gaagaagttg ggactacctc aatctagtag agaggcattt
     2701 gatttgttgg ttgatgcaga tctacttagt acagaactgg caagtaagtt aaaagcaatg
     2761 gttgggtttc gaaatattgc tgttcatgat tatcaaagtg taaacttaga tattgttcgt
     2821 caaattattg aaaaacatct aaaagatttt aaactattta cgaaagaagt tatgggaatt
     2881 ctagacctat aaaagtacaa atcttagaac catgattaaa gtggagaaca ctgtagtaac
     2941 tgtatatcat gaggaggaaa gtacttctgt ggacccttta cttgctttaa caattaaaaa
     3001 caaattaagc gttacatgaa catattgaaa aaaggcatct taaggtgtct tttttgtttt
     3061 gaaaattttt tacatatttt gccgtaaacg aaacataata ttactaaaat cagtggaggt
     3121 gtgtaagaaa tgcaaattcg cgaaaataat aattcaaacg aaatcttgat agcagctgca
     3181 gtaagtgcta ttgagcaaat gaaatatgaa attgctcagg agtttggtgt tacacttgga
     3241 ccggattcaa cagcacgagc gaatggttca gtaggtggag aaattacaaa acgattagtt
     3301 cgtatggcgc aagaacaatt atcaggtcaa tataaaattc actaaagtta aaggtatatc
     3361 ctgttgaaag gatatacctt tttaattttt tgaaagagga taatgaaatt ttgattgtta
     3421 gttaagttta gaaattgcaa ttttagctgt gaaattcatc ttcttcatgt acgaagaatt
     3481 tgcacctgct tgaaattgta cagaaatatt accgtctttt tgtactttga atacgcggcc
     3541 atatggtgaa ttagcattgt aggtgccaat tacttggtta tctgcatcaa agaccgtata
     3601 agaaattggg aacccagaat tttgtacaaa gactgtaagt tcttctcctt tattgagatt
     3661 aaatgtttcc ttttgggaca cttcataaga actcatagag taatattttg taacttcttg
     3721 aatttctaaa ttcgtagttg ctgcactcgc ttttggtgcc tctgctcctg gtaagttcat
     3781 acctgatagt actgcaattc ctagtgcccc tgctaaaata acttttttca taatgctagt
     3841 tcctccttta atatattgtg agatttgaaa aggaagtgaa aatatcacct actgaaacca
     3901 ttatagttac aatgataatt attatcaata cgaaatgcta tgcaattttg catacatgaa
     3961 gtacagcaaa aggccaatca atttaaattt catgtagttt tttgctgctt ttcacgtttt
     4021 attacgtaca atggttttgg tgcaaaaaaa taattgctac tttatgtttc taactatcat
     4081 ttttgcatca gaatcataaa aagcactttt taatgtttta aaagtgcctt tatgttggta
     4141 ttaattatgt agttttataa cttttgacca aattctatta ttaagaatgc tattgtatta
     4201 tgtagttcat gtatccaaaa aacaatccag aaagcagaca tactttttgg gtcgttataa
     4261 tttttttctt gatatataga atatgaataa ttaaatacga tccccataag tgttgctgca
     4321 gctatatatg taatatcaaa ttgatgagtt aatccgaata tgattgacgc tataagaata
     4381 gtaagtatat tacgttctga tatatatgag cttagttgta aaatgaaaaa aagtactttt
     4441 tgaaatagta aagtttcaag aaatggagca ataattaaca ctaataaaat gtgttcaata
     4501 attgactcat ttattgatgg attcgcttgt gaaggtggta aaaataaatt aagtggaata
     4561 gtaattaaaa aactaattat ggtcataagt aaaataaatg gaattttatt taaattagaa
     4621 agaaaattat gtatattcct gaacatttat attatatctc cttagttatg aaattcaaca
     4681 aaatccaata actagttatt ggattttgtt gaattttatt tatttagttt atggtcttcc
     4741 accataatgg ccagcttcaa ttcttccggc atttcccata tcgttaccta atcttccacg
     4801 acaatagtta tcgcgtaaac aatggttggc agcttcataa actttagata aaattcttat
     4861 tgcaccacca tttgtttgta attctttttc agttaattcc tgaaagtttg ttgataattt
     4921 ttcattcatt acatccatct ccttaaaaaa ataaattgtt gattctttat atacgataac
     4981 ataaatgttc tgtaaattta atgtatatgc aaaattgcat atttagtctt ttttctttat
     5041 attcggatat aatcaaaaca gatataaaaa tacaaggaaa agaggctgcg attttgttca
     5101 caaaaaagat ttcttatata agacaacagg atttaaaaga ttgtggtcca gcgtgtcttg
     5161 caatgatttc tcgttatcat ggttttacta tgtctatatc aaaaattaga gaaatagcag
     5221 gtactgattt agaaggaacg aatataaaag gtttaataga agctggagag aagttgggtt
     5281 ttgaggtaaa aggagtaaaa gctacaaatt tcgaagcatt taaagaaatt cctttacctg
     5341 ctattgcaca tgtagttatg gatggtggtc ttcttcatta tgtagtaatt cataaagtta
     5401 aaaataataa aatatatatt gtggatcctg aaaaaggatt tgctacatat tcattagagg
     5461 aatttggaca aatatggact ggtatcttag tacttatgac tcccagtggg gattttcaaa
     5521 caggtaaatc atcacaaagt acattatctc gttttatatt tttattaaga caacaaacta
     5581 atttattggt acctttattt tttgcttcca tcttttttaa tttatttgga ttattaggag
     5641 cattttattt taaattttta attgatgata ttgtgacaaa ccagttgtta cagacacttc
     5701 atattgtatc aattgggatt gttatattgt atatttttaa agtttttcta tcctattttc
     5761 gtactcattt aatcttatat ttaagtcgac gaatagacat tcaattgatg cttggatatt
     5821 atcgccatgt tgtaggatta ccgatgaatt tttttgaaac aagaaaagta ggggaaatca
     5881 tttctcgatt tatggatgca agcaaaataa gagatgcatt atcaacaata acagttacat
     5941 taatgattga tacggtaatg gttacattag gagctgtttt attatatata catagtcccg
     6001 ttttatttgg tgttacttta ttattaattc ccttttatat aggcattatt tttgcttttc
     6061 gtaaaccata tcaacatatt aatagagaag agatggaaag taatgcaaaa ttaaattcat
     6121 atttagtaga gtctttaaat gggattgcga ctataaaatc ttataacgct gaaaaagaag
     6181 ttttttttca aacagaaaat cgtttcgtta atttattaag atatgtattt aaaagaggaa
     6241 tgttatctaa tttacaaggt tctattaaga tggggcttga attaattggg gggacagtta
     6301 ttctttgggt tggtgctatg caagtattaa aaggaagtat gactataggt gaactgatta
     6361 catataatgc ccttttagga tattttttaa atccgattga aaatttaata ggaattcagc
     6421 caactatgca atctgcgttg gttgcaggag aacgattaaa tgaaatcttt gatttagatg
     6481 ctgaaaaaga tgaaaaagaa cataagaaag tttctcctaa acagttatct ggaaaaattg
     6541 aatgttcaaa tgttacattt cgttatggaa cacgtaagaa tgttttaaac aatataagtt
     6601 tttctataga acaaggtagt caagtggctt ttgtaggaga aagtggttcc ggaaaaacaa
     6661 caatttcaaa attgttaatg aattattatg aagcgcaaga aggagttata tattatgaag
     6721 attatcatat taaagaaata aatcgtactg cattacgaga tcggattgct tatgtttctc
     6781 aagaatcatt tttctttagt gcgtctattt atgaaaattt acgttttgga ttagaacgac
     6841 aagtttcgtt agaagagatg atacatgtat gtaaattagc ttgtatacat gagtttatta
     6901 gtgaactacc tttaagttat gaaactttat tagaggaaaa tgcttcgaat ttatcgggtg
     6961 gacagagaca acgtttggca attgcgagag cgttattaaa gaaaccggat atcttaatat
     7021 tagatgaggc cacaagtaat ttagattcta caactgaaaa acatattacg gatatgttaa
     7081 aagaattagg tttacaagga gtaacagtta tcatgattgc gcatagatta agcacaattc
     7141 agcatgcaaa tcgaattttt gtaatggaaa aaggaaatat tatagaacga ggttctcatg
     7201 aagaattatt attccataaa ggtgagtatt atcgtctttg gaagaaccaa acagttcacg
     7261 tagaacatat agaatcacaa tcatatgcaa tgcgaggtgg tcaatatgag taaaatatat
     7321 tcttttgatc aattaacaga tagtgtggag ttattggaaa aaaggccccc tcggtttatt
     7381 acgggattac tatgttttct ttgtataagt ttattaggat ttatgatttg ggcatatgtt
     7441 ggaaaagtag acattgtgag taaaggaaca gcaacaatac aaagtaagtc agatataagt
     7501 gtgtctcgaa ctcaaattcc aggtgttgta gatactgttt ctgtaaaatc aggagatgcg
     7561 gtgaaaaagg gagatacttt gcttcaatta aaaaaccagg aatcaatgga taaacaaaat
     7621 caaatggatc aaattgttaa gcatttagaa ggacaaaaag agatgctaga acaattgaaa
     7681 aagagtatta aatcccataa attatatttt actgatgaag tggataaaaa aataagggaa
     7741 gaatataaag catatgagca aggctatcaa tctttgcaaa atgaaaaaga gaatgagata
     7801 aatgtgatag gtaatagtaa attatcaaat gaacaagatg agattttaca aggattgatt
     7861 gcagaaaagg aaaatataca aagagaaatt aaatctataa ataaacagaa agataaagaa
     7921 aacatattag atgaacaaaa acaagtattg gatgataaaa ttcaatcttt agaatctcaa
     7981 caaaataata tagaaaaaag aatagcgcaa agaaaaggaa cgttagatag tgaaagaaag
     8041 aaagttgata caacgaagga agggaaacaa aaacaaaagg aatacgcttt aaatcaatat
     8101 agagaaagtg ctatagtttc tgtaaatcag cgtattcaat ctttagagca agagatattt
     8161 ataaaaaaac aagacttgtc tggattacat aatcaaaatg aaacgatgat tataaaagca
     8221 catcaagatg gagttgtaca attcccttcc attttacaac cgggtgattt aatagattca
     8281 ggtcaagagg tcgtttcaat tattccgaaa gaagatgaaa aaagggtaaa aattttgtta
     8341 tctgctcaag agataagagg gattaaaaaa ggggataagg tacagtattc gtttaagtta
     8401 aataaaacgg ataaacaaat gggaaaagtt acgtacatat cggcacatcc tatttttgat
     8461 aaagattcta aagggtatgt ttatgaattg gaggcaacaa ttgaaaaaca ggtattgtat
     8521 gagctacata caggaatggt aggcaaagct tctgtagtga caggagaaga acagatatgg
     8581 agatttatat taagaaagtt agattttata tctaattaat agagagtaat attgagttta
     8641 catgttaatt tggaataaag ttacgatgtt ttattccttc aagtaaaaaa atataagtga
     8701 gttttgatta aactttttta taaaaattgt ataatgtttc acaaaatagg aatccgtttt
     8761 aattaatttt tttcaaaatg gatgctttca aaacatattt aagtgaaaat gtatcttgga
     8821 cagaagctgc tgggtttcca tatgcgggca cttatatagg tcctgatgaa gtagtgaaaa
     8881 atgtacatga acgtcttgga actgaatggg ataactatag cgctaaagat gaaatttatg
     8941 cttttaataa caatactgtt atcgtgtatg gaaaatatag tggaacatat aaagtaacag
     9001 ggaaatcatt catagcagat ttttgtcatc tttatgagtt tgatgagaac gataaggtta
     9061 agaagtttat tcaaattgtg gacagtgcaa ctgttaatga ggtattaagt tagagttacc
     9121 agtttcaaat atcaacattt acctttaaca gagccaaaaa ttaaaacaag gtcccttaat
     9181 catgttaaag ggaccttgct cacgcaaatg atctgtaaat caatttatct ttgatataca
     9241 gatcatttgt atgataattt ttgtgaataa ttgctaaata gtgaacttac ataaatgctt
     9301 ttagaagttc ttgaattaat gggattgctc cacctataaa ttttaaaact gccattgcga
     9361 tttccatatt atgctctcct cttttttcgt attaagatgt gataaatctt tataatctta
     9421 ttatagtaag cgcttacaat tataacaatg aattttttta tgcaatattg catatttata
     9481 cactagatct ctattattca ttttttaatg gtactatagc gaggtgagag tctttaaggg
     9541 ttctccttaa taggaggaaa tatggaaaga gtgcaggcga tttacagtaa tcaaaaattt
     9601 aagagttggg ttaaacaggt attagctgta ggaggagtaa tagggtgctg ctatttggtg
     9661 ttatatgttt tctgtttctt agttatcatg taaatattat acaactaaac tagtagagtc
     9721 ttgcctagag aggtaaggct cttttattgt agtacctgga gatgggcaaa caatatacaa
     9781 tacttcttag gtttgtcaga ttactttgta tcgtgccttg tttctctata ttctagaaca
     9841 ctaactccta tcattgagag ttaataaact tctatttaaa gtttccattt atattgaaaa
     9901 cagaattcta ttgataaaaa atatgttttt actagataca tattaaaaag acaagcataa
     9961 attatattag attttagcaa aatctttttg ccttggaaaa ggatttaaat cttacttata
    10021 gatttgattc ctttttatat accataatcc ttatattact acgtaaatat aatacagtaa
    10081 taatgattta aaagcaagaa tattagtagg ggtacgttca cacatccatc aacttaagat
    10141 tttaaagtag ctcgtgaact actcgccact tagcaaagct tgaagtggga gcttctcagt
    10201 tccacgacga aagtaccctt tcgtctccct gagcgttact tcggggtgtt ccacccctag
    10261 atgtccaacg cttggacgct cttttggtac ggttgatatt ttacgcagga accgaatggc
    10321 ttggttttcg cactctattt tcttcctgca acctcatata tcaagttatc aaagaacttc
    10381 atatagctag tttatcaaaa gtttttggct atgccaaacc gaaattcatc tcccacctac
    10441 cgttgggcta cgcccttcac acgcttgagg aaggagaatt ctttcgggga attagttaaa
    10501 ttaaaatcat attggctaac gtaaagaata ccaatcattt acttctccta tcagcacaag
    10561 taaatgattg gtatttctca ttaatattgc taagtctgaa ctacgagcag aatactttat
    10621 ttcattcttt tttcccttac agtttgatct tgtaatcgct gttgttcttt tatcagtcat
    10681 tcctacataa gcgttggata ttttgcaggt ttgtccagac tgaaaagagc ttattaaacc
    10741 atccattatt cacgttcttc ttcacatatt tgtaacacaa ttattttttt gttgttaaaa
    10801 ctagttttta agaagcatat gctttagcaa ctgcagaaat tctttatcac ttttatcatc
    10861 ttttatgtaa tcctgttcgt agttaaaaat cattcgtact aacataaaag ctgtaagtgg
    10921 taatggtacc ttatgcaaag ctccttgctg ataacctttt tcgagcaaat tacttatcat
    10981 tttaattgct tcttcttcag actttttaaa acaagctgta atttcgggtg aatggtactc
    11041 tttatattct tgaaagagtg tagaaacaaa cggatcttga tgatattcta ggctgatata
    11101 atgaaggatt ttttgtattc gaagtaaaac atctttttcc tctttatata aatcaaataa
    11161 ttgtgaacgt ataagctgaa tatgacgcat atagcagtca cataatattt cagttttatc
    11221 tgtatagtaa taataaatag tacctttacc aactttagct ttctttgcaa tttcatcgac
    11281 cgttgtgtta cggacaccac ccatagagaa cagtattgtg gcagattgaa tgattttcat
    11341 ttttttattt tctttttcta atttgatcat tgtctacacc ctatccttga aatatttagc
    11401 gaagttttct ttcattacat tctgtatcag tccatttgta attttttaac tcctaataga
    11461 aagtcctcta tttgcttctt atagaataac atacaccatt ctcatttcta gtatataact
    11521 ataatagaat gcaactttat aacttaggat aaaaatgaaa gtttaaggta ataacttttt
    11581 aatcagacga tgagaattca agtgaaaggg gacagtgatt tctttcttga ttgaattatt
    11641 tgatttttta ttcaattcgt tttattatat aaacatgact aaaagatgtt tttagtcgga
    11701 tttttttacg gaaaaccgac tgatatttaa ttttagtcgg tttctatact atttgagaga
    11761 aggtagacac tatgtgtgga attgcagggt ggatagactg gtctaaagac ctgtctcatg
    11821 aacaagaaac gttaaaaaaa atgacagatg ctatcactca ccgagggcct gatgcagagg
    11881 ggcattggct ttcaaaacga gcagcattag gtcatcgacg tctcatcgtc attgatcctg
    11941 aaggtggcct tcaacctatg ttgtataaag atggaaatga tacgattggc cttacgttca
    12001 atggagaaat ttataactat caagaattac gtaaagaact tgaggggaaa ggtcatgaat
    12061 ttcagacaaa gtctgataca gaggtattac tacatgctta tttagaatgg caagaagatt
    12121 gtgttcaaca cttaaatggg atttttgctt ttggtatttg ggatgaaaga tttgggaaat
    12181 tgatgcttgg tcgtgatcat ctaggtgtga agcctttatt tttcgctcaa agaggaagcg
    12241 caattctctt tggttcagag ttaaaagttt tgctttcgca tccgcttgta aatgcagaag
    12301 tagataaaga agg
//
