LOCUS       c00049_NZ_NFJN..        3259 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_NFJN01000053.1 Gordonibacter urolithinfaciens strain An234A
            An234A_contig_53, whole genome shotgun sequence.
ACCESSION   NZ_NFJN01000053
VERSION     NZ_NFJN01000053
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 15:16:32
            Original ID  :: NZ_NFJN01000053.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3259
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..3258
                     /NRPS_PKS="Domain: AMP-binding (36-407). E-value: 4.2e-85.
                     Score: 277.6. Matches aSDomain:
                     nrpspksdomains_ctg49_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (826-891). E-value: 2e-18. Score:
                     58.2. Matches aSDomain: nrpspksdomains_ctg49_1_PCP.1"
                     /NRPS_PKS="Domain: Heterocyclization (910-1084). E-value:
                     2.4e-30. Score: 97.8. Matches aSDomain:
                     nrpspksdomains_ctg49_1_Heterocyclization.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     276.8; E-value: 4.5e-84)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg49_1"
                     /sec_met_domain="Condensation (E-value: 5.6e-20, bitscore:
                     57.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.9e-79, bitscore:
                     251.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.3e-12, bitscore:
                     33.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DWSEPFPDLLPPSQRAARAEANSEGADLGGGTLHGRFFARAQAEP
                     DAPALRWGADGEATYGELAGRALRIAGGLAAAGVGEGDRVAVRLPKGPGQVAAVLGVLA
                     AGAAYVPVGVSQPEARAALILEQAGASLCVDSTELARLEAADPLPAPAGPSERALAYVI
                     YTSGSTGVPKGVAVSHAAAWNTIADVVRRWGMGPGDAVLGVSALDFDLSVFDIFGALSF
                     GAAVVLPDEEERRDASAWRELVSSRGVTVWNSAPALLDVMLDDGPGAAAGLRLALVSGD
                     WVPLGLRSKLPEGVLLVAMGGATEAGIWSNYFEVGEVDPSWSSIPYGRPLSNQSFRVVG
                     EDGLDCPDWVPGELWIGGGSLADGYLGDPGKTAASFVEDGGRWYRTGDMGRYWPGAVLE
                     FLGRKDSQQQVKIRGHRVELGEVEAAVAGCPGVKGAVAVVAGEREHLFAVAIPAISEAP
                     IASISERSPVENREAEHTRLTALSNILIKERGLSNDRLFTNEQSDESSSRYHAVMEMLS
                     RNTSNCSTVCRDNTLEEAADFYEKILTEAADPSTLYESDRFSPEALFLSRNSSQRIIKD
                     AIATIKAMAETLGRKLKVADFGCRSGRISALLLPEVADTISEYHLLDTSQSMLDMAKTR
                     LDDFSEKVRTAKLTKVVDQDIHARFDVVVSINAIHTMEEPSEAISALGETLAADGRAFI
                     VDPTEFAPSAMVTAAIAQCAFGDGEPSGLLSPEKWIDSLSEKGLRADWVKIDDGLFISL
                     SSRTSPPIDLTLLRSTLEKEVPAYMVPEHLGILPSQPLSRNGKVDKGSVKDWAELYCSF
                     CDASDNYIEPNGEIEKKVSSIWSKFLGGRRVGRYDGFFEYGGDSLSATRMISALSKIGI
                     KASLKELFASGNLADFCKGLSISSPNNSKAITINEELRFEPFDLTEVQRAYTIGRTSGL
                     DLGKVGSYCFFMFETSEYSHDRFASAWRILLERHGALRTLLVKNGEKQQVYRSSDLNLP
                     LAVQNAMDSKDARKIVEQAMSHRSIDLTKSPGFEALSAVYSSGAVVGIGFDNTMVDGVS
                     MSTVLAELAELYENPGADLPGIGLTFRDFVVGHRRDGR"
     protocluster    1..3259
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:3258](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3258
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..3259
                     /SMILES="NC(CS)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3259
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     aSDomain        109..1221
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg49_1_AMP-binding.1"
                     /evalue="4.20E-85"
                     /label="ctg49_1_AMP-binding.1"
                     /locus_tag="ctg49_1"
                     /protein_end="407"
                     /protein_start="36"
                     /score="277.6"
                     /specificity="consensus: cys"
                     /tool="antismash"
                     /translation="FFARAQAEPDAPALRWGADGEATYGELAGRALRIAGGLAAAGVGE
                     GDRVAVRLPKGPGQVAAVLGVLAAGAAYVPVGVSQPEARAALILEQAGASLCVDSTELA
                     RLEAADPLPAPAGPSERALAYVIYTSGSTGVPKGVAVSHAAAWNTIADVVRRWGMGPGD
                     AVLGVSALDFDLSVFDIFGALSFGAAVVLPDEEERRDASAWRELVSSRGVTVWNSAPAL
                     LDVMLDDGPGAAAGLRLALVSGDWVPLGLRSKLPEGVLLVAMGGATEAGIWSNYFEVGE
                     VDPSWSSIPYGRPLSNQSFRVVGEDGLDCPDWVPGELWIGGGSLADGYLGDPGKTAASF
                     VEDGGRWYRTGDMGRYWPGAVLEFLGRKDSQ"
     PFAM_domain     109..1221
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_1_0001"
                     /evalue="1.10E-77"
                     /label="ctg49_1"
                     /locus_tag="ctg49_1"
                     /protein_end="407"
                     /protein_start="36"
                     /score="261.5"
                     /tool="antismash"
                     /translation="FFARAQAEPDAPALRWGADGEATYGELAGRALRIAGGLAAAGVGE
                     GDRVAVRLPKGPGQVAAVLGVLAAGAAYVPVGVSQPEARAALILEQAGASLCVDSTELA
                     RLEAADPLPAPAGPSERALAYVIYTSGSTGVPKGVAVSHAAAWNTIADVVRRWGMGPGD
                     AVLGVSALDFDLSVFDIFGALSFGAAVVLPDEEERRDASAWRELVSSRGVTVWNSAPAL
                     LDVMLDDGPGAAAGLRLALVSGDWVPLGLRSKLPEGVLLVAMGGATEAGIWSNYFEVGE
                     VDPSWSSIPYGRPLSNQSFRVVGEDGLDCPDWVPGELWIGGGSLADGYLGDPGKTAASF
                     VEDGGRWYRTGDMGRYWPGAVLEFLGRKDSQ"
     aSModule        109..2673
                     /complete
                     /domains="nrpspksdomains_ctg49_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg49_1_PCP.1"
                     /locus_tags="ctg49_1"
                     /monomer_pairings="cys -> cys"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     aSDomain        175..1308
                     /aSDomain="TIGR01733"
                     /aSTool="tigrfam"
                     /description="AA-adenyl-dom: amino acid adenylation domain"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg49_1_0001"
                     /evalue="1.40E-104"
                     /identifier="TIGR01733"
                     /label="ctg49_1"
                     /locus_tag="ctg49_1"
                     /protein_end="436"
                     /protein_start="58"
                     /score="348.1"
                     /tool="antismash"
                     /translation="TYGELAGRALRIAGGLAAAGVGEGDRVAVRLPKGPGQVAAVLGVL
                     AAGAAYVPVGVSQPEARAALILEQAGASLCVDSTELARLEAADPLPAPAGPSERALAYV
                     IYTSGSTGVPKGVAVSHAAAWNTIADVVRRWGMGPGDAVLGVSALDFDLSVFDIFGALS
                     FGAAVVLPDEEERRDASAWRELVSSRGVTVWNSAPALLDVMLDDGPGAAAGLRLALVSG
                     DWVPLGLRSKLPEGVLLVAMGGATEAGIWSNYFEVGEVDPSWSSIPYGRPLSNQSFRVV
                     GEDGLDCPDWVPGELWIGGGSLADGYLGDPGKTAASFVEDGGRWYRTGDMGRYWPGAVL
                     EFLGRKDSQQQVKIRGHRVELGEVEAAVAGCPGVKGAV"
     CDS_motif       298..336
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0001"
                     /evalue="2.30E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg49_1"
                     /protein_end="112"
                     /protein_start="99"
                     /score="11.5"
                     /tool="antismash"
                     /translation="LGVLAAGAAYVPV"
     CDS_motif       478..537
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0002"
                     /evalue="1.30E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg49_1"
                     /protein_end="179"
                     /protein_start="159"
                     /score="33.0"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGVPKGVAVSH"
     CDS_motif       652..684
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0003"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg49_1"
                     /protein_end="228"
                     /protein_start="217"
                     /score="-2.5"
                     /tool="antismash"
                     /translation="GALSFGAAVVL"
     CDS_motif       1039..1128
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0004"
                     /evalue="1.20E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg49_1"
                     /protein_end="376"
                     /protein_start="346"
                     /score="46.4"
                     /tool="antismash"
                     /translation="PDWVPGELWIGGGSLADGYLGDPGKTAASF"
     CDS_motif       1198..1269
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0005"
                     /evalue="3.70E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg49_1"
                     /protein_end="423"
                     /protein_start="399"
                     /score="28.8"
                     /tool="antismash"
                     /translation="FLGRKDSQQQVKIRGHRVELGEVE"
     PFAM_domain     1786..2073
                     /aSDomain="Methyltransf_12"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF08242.14"
                     /description="Methyltransferase domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_1_0002"
                     /evalue="3.50E-11"
                     /label="ctg49_1"
                     /locus_tag="ctg49_1"
                     /protein_end="691"
                     /protein_start="595"
                     /score="43.8"
                     /tool="antismash"
                     /translation="DFGCRSGRISALLLPEVADTISEYHLLDTSQSMLDMAKTRLDDFS
                     EKVRTAKLTKVVDQDIHARFDVVVSINAIHTMEEPSEAISALGETLAADGR"
     aSDomain        2479..2673
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg49_1_PCP.1"
                     /evalue="2.00E-18"
                     /label="ctg49_1_PCP.1"
                     /locus_tag="ctg49_1"
                     /protein_end="891"
                     /protein_start="826"
                     /score="58.2"
                     /tool="antismash"
                     /translation="EKKVSSIWSKFLGGRRVGRYDGFFEYGGDSLSATRMISALSKIGI
                     KASLKELFASGNLADFCKGL"
     PFAM_domain     2485..2667
                     /aSDomain="PP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00550.27"
                     /description="Phosphopantetheine attachment site"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_1_0003"
                     /evalue="1.30E-06"
                     /label="ctg49_1"
                     /locus_tag="ctg49_1"
                     /protein_end="889"
                     /protein_start="828"
                     /score="28.7"
                     /tool="antismash"
                     /translation="KVSSIWSKFLGGRRVGRYDGFFEYGGDSLSATRMISALSKIGIKA
                     SLKELFASGNLADFCK"
     aSDomain        2731..3252
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg49_1_Heterocyclization.1"
                     /evalue="2.40E-30"
                     /label="ctg49_1_Heterocyclization.1"
                     /locus_tag="ctg49_1"
                     /protein_end="1084"
                     /protein_start="910"
                     /score="97.8"
                     /tool="antismash"
                     /translation="EPFDLTEVQRAYTIGRTSGLDLGKVGSYCFFMFETSEYSHDRFAS
                     AWRILLERHGALRTLLVKNGEKQQVYRSSDLNLPLAVQNAMDSKDARKIVEQAMSHRSI
                     DLTKSPGFEALSAVYSSGAVVGIGFDNTMVDGVSMSTVLAELAELYENPGADLPGIGLT
                     FRDFVVGHRRD"
     PFAM_domain     2821..3243
                     /aSDomain="Condensation"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00668.22"
                     /description="Condensation domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg49_1_0004"
                     /evalue="1.90E-11"
                     /label="ctg49_1"
                     /locus_tag="ctg49_1"
                     /protein_end="1081"
                     /protein_start="940"
                     /score="43.4"
                     /tool="antismash"
                     /translation="FMFETSEYSHDRFASAWRILLERHGALRTLLVKNGEKQQVYRSSD
                     LNLPLAVQNAMDSKDARKIVEQAMSHRSIDLTKSPGFEALSAVYSSGAVVGIGFDNTMV
                     DGVSMSTVLAELAELYENPGADLPGIGLTFRDFVVGH"
     CDS_motif       3130..3180
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg49_1_0006"
                     /evalue="1.40E-03"
                     /label="C3_DCL_135-156"
                     /locus_tag="ctg49_1"
                     /protein_end="1060"
                     /protein_start="1043"
                     /score="11.3"
                     /tool="antismash"
                     /translation="VDGVSMSTVLAELAELY"
ORIGIN
        1 gactggtcgg agccgttccc cgacctgctg cccccttcgc agcgcgcggc gcgcgccgag
       61 gcgaactcgg agggggccga cctcggcggc gggacgctcc acggacggtt cttcgcccgc
      121 gcccaggccg agccggatgc cccggcgctg cggtggggag ccgacggcga ggcgacctac
      181 ggggagcttg ccggccgcgc cctgcgcatc gccggggggc tcgccgccgc cggcgtgggc
      241 gagggcgacc gcgtggccgt caggctcccg aaggggcccg gccaggtggc agccgtgctg
      301 ggcgtgctgg ccgccggggc ggcgtacgtc cccgtcggcg tctcccagcc cgaggcccgc
      361 gcggcgctca tcctcgaaca ggcgggcgcg tccctgtgcg tcgactccac cgagctggcc
      421 cgcctcgagg cggccgaccc cctccccgcg cccgccggcc cctccgagcg cgcgctggcc
      481 tacgtgatct acacgtccgg ctccaccggg gtgccgaagg gggtggcggt gagccacgcc
      541 gccgcgtgga acaccatcgc cgatgtcgtg cgccgatggg gcatggggcc gggagatgcg
      601 gtcctgggcg tgtcggccct tgacttcgac ctctccgtgt tcgacatctt cggggccctc
      661 tcgttcggag ccgccgtcgt gctgcccgac gaggaggagc gccgcgacgc atcggcgtgg
      721 cgcgagctcg tctcgtcccg gggcgtgacg gtgtggaact cggcccccgc cctgctggac
      781 gtgatgctcg acgacggccc cggggccgcg gccggtctgc ggttggccct cgtctccggc
      841 gactgggtgc cgctgggtct gcgctcgaag ctgcccgagg gggtgctgct cgtggccatg
      901 ggcggcgcga ccgaggccgg catatggtcg aactacttcg aggtcgggga ggtcgatccg
      961 tcgtggagct cgatccccta cggcagaccc ctctccaacc agtccttccg cgtcgtgggc
     1021 gaggacggcc tcgactgccc cgactgggtg cccggcgagc tgtggatcgg cggggggagc
     1081 ctggccgacg ggtatctggg cgatccgggg aagacggccg cctccttcgt cgaggacggg
     1141 gggcgctggt accggacggg ggacatgggc cgctactggc ccggagccgt cttggagttc
     1201 ctcggtcgca aggattccca gcagcaggtg aagatacgcg gccacagggt ggagctgggc
     1261 gaggtcgagg ccgccgtggc cggatgcccc ggggtgaagg gggccgtggc cgtcgtggcc
     1321 ggcgagcgcg agcatctttt tgctgtcgca atccctgcca tcagcgaagc ccccatcgcc
     1381 tcaattagcg agagatcgcc agtcgagaac agggaagctg aacacacgcg gctcactgcg
     1441 ctttctaata ttcttatcaa agagcgaggc ttgtcgaatg acaggctttt tacgaacgag
     1501 cagagtgacg aatcttcttc acgatatcac gcggttatgg aaatgctctc tcgcaacact
     1561 tcaaattgct caacagtatg ccgagacaac acattggaag aagccgctga tttctatgaa
     1621 aagatactga cagaagccgc agacccttcc acgttgtacg aaagcgacag gttctctcct
     1681 gaagccctct ttctctctcg caactcgtcc caaagaatca tcaaggacgc aatcgccaca
     1741 atcaaggcaa tggctgaaac gcttggccgc aaacttaaag tcgctgattt cggatgccga
     1801 agtggacgca tatcagccct gttgctcccc gaggtcgcag atactatctc ggaataccat
     1861 ctgctcgaca cttctcaatc tatgctagat atggccaaaa caaggctaga cgacttttca
     1921 gaaaaagtga gaacagcaaa gcttacaaaa gttgtcgatc aagatattca tgcacgtttc
     1981 gatgttgtgg tgtcgataaa cgcaattcac accatggaag aaccttctga ggcaataagt
     2041 gctcttggag aaaccctagc agcagacggc agggctttca ttgtggatcc caccgagttt
     2101 gccccttccg caatggtaac agctgcaatt gcccagtgcg catttggcga tggggaaccg
     2161 agcggtctgc tttctcccga gaaatggatc gactctcttt cggagaaagg attgcgtgcc
     2221 gactgggtaa aaatcgacga tggcctgttc atctctctgt ccagccgaac ttctccacct
     2281 atcgacctca cacttctccg ctctactcta gaaaaagaag ttcccgcata tatggttccc
     2341 gagcatcttg gaatcctacc ttctcagccg ctatccagaa acggaaaagt ggataaagga
     2401 agcgttaaag actgggctga attgtattgc tctttctgtg atgcatctga caattatatt
     2461 gagccaaatg gagaaatcga gaaaaaggtt tcgtctatat ggtcaaaatt ccttggagga
     2521 aggcgtgtcg gccgctacga tggatttttt gaatacggag gagacagcct aagtgctacg
     2581 cggatgattt ccgctctttc aaaaattggc atcaaagcaa gtctaaagga gcttttcgcc
     2641 tcaggaaatc ttgcagactt ctgcaaaggg ctttccattt cgtctcctaa taactccaag
     2701 gcaatcacta tcaatgaaga gttgagattt gagccgttcg acctcaccga ggtacagagg
     2761 gcatacacca taggaaggac cagcggtctc gacttgggca aggtaggatc ctattgcttc
     2821 ttcatgtttg aaactagcga atacagtcac gacagatttg catcagcatg gaggattctg
     2881 ctcgagcgtc acggagcctt gcgcactctt ctagtcaaga atggcgagaa gcagcaggtg
     2941 tatcgatcat ctgatctgaa tctacctctg gctgtccaaa atgcaatgga ttcaaaagat
     3001 gctcgtaaaa tagtcgaaca agcaatgtcg catagatcga tagatctaac gaaatctccc
     3061 ggctttgaag ctctctcggc agtttatagc agcggtgctg ttgtcggtat tggcttcgac
     3121 aacactatgg tagacggcgt gagcatgagc accgttctcg ccgaactggc cgaactctac
     3181 gagaaccccg gggccgacct ccccggcatc ggcctgacgt tcagggattt cgtcgtcggg
     3241 caccggagag acggtcgcg
//
