MIBiG Protein Description MIBiG Cluster MiBiG Product % ID % Coverage BLAST Score E-value
BAB69698.1 iturin_A_synthetase_A BGC0001098 NRP + Polyketide 35.0 100.9 334.0 3.7e-91
AAF08795.1 MycA BGC0001103 NRP + Polyketide 33.0 100.9 331.0 2.4e-90
AHD05679.1 putative_non-ribosomal_peptide_ligase/_polyketide_synthase_hybrid BGC0000402 NRP 33.0 101.7 331.0 4.1e-90
ABS74181.1 bacillomycin_D_synthetase_A_ BGC0001090 Polyketide + NRP:Lipopeptide 34.0 100.9 330.0 7.1e-90
AHI59107.1 locillomycin_synthase_D BGC0001005 NRP + Polyketide 33.0 102.4 316.0 1.1e-85
PPE_RS11475 aminotransferase_class_III-fold_pyridoxal_phosphate-dependent_enzyme BGC0001993 NRP 31.0 103.2 302.0 1.2e-81
AID65222.1 putative_aspartate_racemase BGC0000335 NRP 32.0 87.5 267.0 7.3e-71
ABM21572.1 crpD BGC0000975 NRP + Polyketide 30.0 90.5 236.0 1.1e-61
ABM21569.1 crpA BGC0000975 NRP + Polyketide 26.0 102.7 232.0 2.6e-60
AAF00957.1 mcyG BGC0001017 NRP + Polyketide:Modular type I 28.0 101.3 229.0 1.7e-59
AAY42397.1 Nonribosomal_peptide_synthetase BGC0001000 NRP:Lipopeptide + Polyketide:Modular type I 29.0 89.7 228.0 2.9e-59
AQH32483.1 hybrid_peptide_synthetase/polyketide_synthase BGC0001667 NRP + Polyketide 27.0 101.7 228.0 2.9e-59
ATP76242.1 NdaC BGC0001705 NRP + Polyketide 27.0 101.7 227.0 8.4e-59
CAM59601.1 non-ribosomal_peptide_synthetase/polyketide_synthase_hybrid_enzyme BGC0000297 NRP:Glycopeptide + Polyketide:Other + Saccharide:Hybrid/tailoring 29.0 100.6 226.0 1.1e-58
AAY42398.1 Nonribosomal_peptide_synthetase BGC0001000 NRP:Lipopeptide + Polyketide:Modular type I 28.0 89.7 226.0 1.1e-58
CAD29795.1 peptide_synthetase BGC0001015 NRP + Polyketide 26.0 102.2 225.0 1.9e-58
AAO62585.1 peptide_sythetase_polyketide_synthase_fusion_protein BGC0001016 NRP + Polyketide 27.0 100.8 225.0 2.4e-58
ATY37596.1 BogI BGC0001532 NRP 29.0 88.3 223.0 9.3e-58
ABD14712.1 cesB BGC0000320 NRP:Cyclic depsipeptide 27.0 90.5 213.0 1.3e-54
AQX77695.1 NocQ BGC0001704 Other 28.0 88.9 210.0 1.1e-53
AKL71650.1 NocQ BGC0001703 Other 28.0 90.0 204.0 4.5e-52
BAE93741.1 putative_acyltransferase_family_protein BGC0000164 Polyketide 30.0 62.2 200.0 8.4e-51
WP_081656241.1 non-ribosomal_peptide_synthetase BGC0001467 NRP:Cyclic depsipeptide + Polyketide:Modular type I 27.0 91.0 198.0 4.2e-50
ADD82966.1 BatY BGC0001099 NRP + Polyketide:Modular type I + Polyketide:Trans-AT type I 27.0 87.8 194.0 6e-49
WP_010639240.1 non-ribosomal_peptide_synthetase BGC0000958 NRP:Cyclic depsipeptide + Polyketide:Modular type I 26.0 85.6 188.0 4.3e-47
AIU36103.1 LglD BGC0000180 Polyketide:Trans-AT type I 25.0 101.7 187.0 5.7e-47
ACR50797.1 acyltransferase BGC0000163 Polyketide 29.0 62.7 186.0 1.3e-46
AEF33080.1 putative_peptide_synthetase BGC0001039 NRP + Polyketide 26.0 86.9 186.0 1.3e-46
AXN93578.1 PuwC BGC0001950 NRP 28.0 91.0 185.0 2.1e-46
AXN93587.1 PuwC BGC0001951 NRP 28.0 91.0 185.0 2.1e-46
AXN93595.1 PuwI BGC0001952 NRP 26.0 109.8 185.0 3.7e-46
AIW82280.1 PuwC BGC0001125 NRP + Polyketide 28.0 90.2 183.0 1.1e-45
AQA28568.1 acyl-CoA_synthase BGC0001663 Polyketide 28.0 87.8 182.0 1.8e-45
DAB41914.1 ArzL_-_PKS_(FAAL,_ACP) BGC0001884 NRP + Polyketide 28.0 88.3 182.0 1.8e-45
SCO70321.1 AMP-dependent_synthetase/ligase BGC0001433 Polyketide:Modular type I 29.0 61.9 181.0 3.1e-45
AWW87429.1 CoA_ligase BGC0001755 Polyketide 25.0 81.5 181.0 3.1e-45
QBC75017.1 acyl-CoA_synthase BGC0001968 NRP 32.0 65.2 181.0 4.1e-45
AAS98774.1 Acyl-ACP_Synthetase BGC0001001 NRP + Polyketide 27.0 87.5 181.0 5.3e-45
WP_026723805.1 AMP-dependent_synthetase BGC0001467 NRP:Cyclic depsipeptide + Polyketide:Modular type I 27.0 89.6 180.0 6.9e-45
AQX77690.1 NocL BGC0001704 Other 29.0 91.0 179.0 2e-44
AFV96135.1 AMP-dependent_synthetase/ligase BGC0001064 Polyketide:Modular type I + Polyketide:Type III 29.0 91.6 178.0 2.6e-44
ACA99172.1 polyketide_synthase BGC0001160 Polyketide:Modular type I 28.0 82.4 178.0 2.6e-44
ARU81115.1 CylA BGC0001566 Polyketide 29.0 91.6 178.0 2.6e-44
AMB48444.1 long-chain-fatty-acid--CoA_ligase BGC0001357 Polyketide 28.0 90.7 177.0 5.9e-44
CAD32910.2 acyl-CoA_synthase BGC0000354 NRP 32.0 51.4 176.0 1e-43
AWI62626.1 nonribosomal_peptide_synthetase BGC0001943 NRP 28.0 90.3 176.0 1e-43
ADQ55476.1 NRPS BGC0000350 NRP:Beta-lactam 28.0 95.6 176.0 1.3e-43
AQX77685.1 NocH BGC0001704 Other 26.0 97.3 176.0 1.3e-43
WP_039806856.1 non-ribosomal_peptide_synthetase BGC0002001 NRP + Polyketide 30.0 63.9 175.0 3.8e-43
CAE53367.1 acyl-CoA_ligase BGC0000440 NRP:Glycopeptide 26.0 69.5 174.0 5e-43
CAG15025.1 Acyl-CoA_synthase BGC0000441 NRP 26.0 69.5 174.0 5e-43
AAY42393.1 Acyl-ACP_synthetase BGC0001000 NRP:Lipopeptide + Polyketide:Modular type I 27.0 86.7 174.0 5e-43
ABL74940.1 NRPS BGC0001048 NRP:Glycopeptide + Polyketide:Modular type I + Saccharide:Hybrid/tailoring 25.0 87.5 174.0 6.5e-43
AKL71645.1 NocL BGC0001703 Other 27.0 89.4 173.0 8.5e-43
ADC45517.1 AMP-dependent_synthetase_and_ligase BGC0000093 Polyketide 29.0 63.3 173.0 1.1e-42
AXN93598.1 PuwC BGC0001952 NRP 27.0 87.0 173.0 1.4e-42
ADN13832.1 Polyketide_Synthase BGC0001164 Polyketide:Modular type I 24.0 107.0 172.0 2.5e-42
AXN93608.1 PuwI BGC0001953 NRP 29.0 85.0 172.0 2.5e-42
AWX24482.1 non-ribosomal_peptide_synthetase BGC0001695 NRP 24.0 87.7 171.0 3.2e-42
CAQ71826.1 non_ribosomal_peptide_synthase,_antibiotic_synthesis;_contains_1_condensation_domain,_1_AMP-acid_ligases_II_domain,_PP-binding,_Phosphopantetheine_attachment_site_and_one_taurine_catabolism_dioxygenase BGC0001189 NRP 30.0 49.4 171.0 4.2e-42
BAK64635.1 putative_CoA_ligase BGC0000135 Polyketide 26.0 88.0 170.0 9.3e-42
ARU08069.1 mlcG BGC0001448 NRP:Ca+-dependent lipopeptide 26.0 82.6 170.0 9.3e-42
EGJ35087.1 Fatty-acyl-ACP_ligase BGC0001163 Polyketide:Modular type I 28.0 82.8 170.0 1.2e-41
AKL71641.1 NocH BGC0001703 Other 26.0 103.0 170.0 1.2e-41
ACG60776.1 NRPS(AL/ACP/C/A/PCP/C/A) BGC0001058 NRP:Glycopeptide + Polyketide:Modular type I + Saccharide:Hybrid/tailoring 29.0 62.5 169.0 1.6e-41
AXN93611.1 PuwC BGC0001953 NRP 30.0 65.3 169.0 1.6e-41
AAZ23074.1 acyl-CoA_ligase BGC0000291 NRP 28.0 77.4 168.0 3.6e-41
AEF16021.1 acyl-CoA_synthase BGC0000379 NRP 26.0 70.9 168.0 4.6e-41
ADJ63842.1 Serobactin_synthetase BGC0000424 NRP:NRP siderophore 25.0 97.9 167.0 6.1e-41
ALV86864.1 Tlo18 BGC0001406 NRP 32.0 50.9 167.0 6.1e-41
CAJ88192.1 putative_peptide_synthetase_NRPS5-4-3 BGC0000151 Polyketide:Modular type I + Saccharide:Hybrid/tailoring 26.0 89.2 166.0 1.3e-40
ACY06269.1 CoA_ligase BGC0001042 NRP + Polyketide 31.0 48.7 166.0 1.8e-40
AHH34188.1 fatty_acyl_ACP_ligase BGC0001162 Polyketide:Modular type I 28.0 84.2 165.0 2.3e-40
ABX37385.1 amino_acid_adenylation_domain_protein BGC0000984 NRP + Polyketide 29.0 51.4 165.0 3.9e-40
WP_006051172.1 AMP-binding_protein BGC0001999 NRP 25.0 73.4 164.0 5.1e-40
EWM62997.1 non-ribosomal_peptide_synthetase BGC0001328 NRP:Cyclic depsipeptide + Polyketide:Modular type I 31.0 65.0 164.0 6.7e-40
ACZ65474.1 palmitoyl-CoA_synthetase BGC0000140 Polyketide 29.0 63.8 163.0 1.1e-39
AAY93445.1 non-ribosomal_peptide_synthetase_PvdL BGC0000413 NRP 28.0 77.2 163.0 1.1e-39
AEA60663.1 pyoverdine_synthetase BGC0000892 Other 28.0 63.9 163.0 1.1e-39
KFL51881.1 beta-ketoacyl_synthase BGC0001711 NRP + Polyketide 26.0 86.7 162.0 1.9e-39
ABI22131.1 putative_non-ribosomal_peptide_synthetase BGC0000422 NRP 27.0 62.5 161.0 4.3e-39
AHH34187.1 fatty_acyl_ACP_ligase BGC0001161 Polyketide:Modular type I 27.0 84.3 161.0 4.3e-39
BAW32322.1 nonribosomal_peptide_synthetase BGC0001630 NRP + Polyketide 25.0 85.4 161.0 4.3e-39
EDT06082.1 AMP-dependent_synthetase_and_ligase BGC0001897 Polyketide 27.0 68.7 161.0 5.7e-39
CBW54660.1 Acyl-CoA_ligase BGC0000971 NRP + Polyketide:Modular type I 25.0 88.0 160.0 9.7e-39
AQW35047.1 Acyl-CoA_synthetase BGC0001675 Polyketide 25.0 83.9 160.0 9.7e-39
AEC04356.1 polyketide_synthase BGC0000178 Polyketide:Trans-AT type I 24.0 104.3 160.0 1.3e-38
CAD17792.1 probable_non_ribosomal_peptide_synthetase_protein BGC0001754 NRP + Polyketide 31.0 49.2 159.0 1.6e-38
QED55419.1 fatty-acyl_AMP_ligase BGC0001984 NRP 25.0 80.4 158.0 4.8e-38
BAO84862.1 putative_long-chain-fatty-acid-CoA_ligase BGC0000414 NRP 29.0 65.5 157.0 8.2e-38
AZF85942.1 long-chain-fatty-acid-CoA_ligase BGC0001963 NRP + Polyketide 27.0 71.7 157.0 8.2e-38
AHF22854.1 MarL BGC0000091 Polyketide 30.0 49.7 156.0 1.1e-37
WP_018960015.1 fatty_acyl-AMP_ligase BGC0002010 NRP + Polyketide 25.0 86.7 156.0 1.1e-37
AHH53502.1 acyl-CoA_synthetase BGC0000439 NRP:Ca+-dependent lipopeptide 27.0 62.3 156.0 1.4e-37
ABP57745.1 DepA BGC0000993 NRP:Cyclic depsipeptide + Polyketide:Modular type I 24.0 77.8 155.0 2.4e-37
CAO98843.1 hypothetical_protein BGC0000023 Polyketide:Modular type I 28.0 64.1 155.0 4.1e-37
AAX31555.1 acyl-CoA_ligase BGC0000336 NRP 31.0 50.2 153.0 9.1e-37
AYM52861.1 AMP-binding_protein BGC0001964 Polyketide 27.0 63.0 153.0 9.1e-37
CAD15508.1 polyketide_synthase/non-ribosomal_peptide_synthetase BGC0001014 NRP:NRP siderophore + Polyketide:Modular type I + Polyketide:Iterative type I 27.0 83.4 153.0 1.2e-36
AWS21283.1 acyl-CoA_synthetase BGC0001934 Polyketide 25.0 88.4 152.0 2.6e-36
AZY91992.1 putative_fatty_acyl-AMP_ligase BGC0002022 Polyketide 25.0 88.4 152.0 2.6e-36
AHN85651.1 Phn2 BGC0000122 Polyketide:Modular type I 24.0 83.5 150.0 1e-35
ABF87031.1 non-ribosomal_peptide_synthetase/polyketide_synthase BGC0000393 NRP + Polyketide:Modular type I 30.0 50.0 150.0 1e-35
AFR69331.1 nonribosomal_peptide_synthetase_SpiA BGC0001045 NRP:Cyclic depsipeptide + Polyketide:Modular type I 30.0 54.0 149.0 1.3e-35
AAN85512.1 nonribosomal_peptide_synthetase BGC0001101 NRP + Polyketide:Modular type I + Polyketide:Trans-AT type I 27.0 63.4 149.0 1.7e-35
CAA16183.1 polyketide_synthase BGC0001063 NRP + Polyketide 28.0 49.5 149.0 2.2e-35
WP_012015338.1 AMP-binding_protein BGC0001458 Other:Aminocoumarin 24.0 73.7 144.0 4.2e-34
WP_053065267.1 type_I_polyketide_synthase BGC0001330 NRP:Cyclic depsipeptide + Polyketide:Modular type I 27.0 64.4 144.0 5.5e-34
ABC70854.1 FadD28 BGC0000365 NRP 28.0 65.8 143.0 9.4e-34
WP_019032757.1 type_I_polyketide_synthase BGC0001331 NRP:Cyclic depsipeptide + Polyketide:Modular type I 26.0 63.6 143.0 9.4e-34
AOE23577.1 FoxBI BGC0001598 NRP + Polyketide 27.0 65.3 143.0 9.4e-34
BAG84248.1 putative_polyketide_synthase BGC0000257 Polyketide 28.0 51.9 139.0 1.8e-32
CAQ43075.1 polyketide_synthase BGC0000970 NRP + Polyketide:Modular type I 25.0 80.4 138.0 3e-32
orf9 acyl-CoA_synthetase BGC0001420 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 27.0 63.4 138.0 3e-32
APZ78722.1 acyl-CoA_synthetase BGC0001420 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 27.0 63.4 138.0 3e-32
ABC35922.1 AMP-binding_domain_protein BGC0001102 NRP:Beta-lactam + Polyketide:Modular type I 28.0 64.7 138.0 3.9e-32
WP_003598544.1 fatty_acyl-AMP_ligase BGC0001991 Polyketide 28.0 50.0 133.0 1.7e-30
AGD80622.1 AMP-dependent_synthetase_and_ligase BGC0000394 NRP 25.0 85.6 130.0 1.1e-29
AEZ53969.1 putative_AMP-binding_domain-containing_protein BGC0000144 Polyketide:Modular type I 24.0 67.4 120.0 8.5e-27
CDE97356.1 plipastatin_synthase_subunit_C BGC0001686 NRP 22.0 100.6 99.0 2e-20
AEW31026.1 short-chain_acyl-CoA_synthetase BGC0000407 NRP 24.0 63.0 97.0 7.7e-20
AAF08801.1 YngI BGC0001103 NRP + Polyketide 25.0 63.0 97.0 7.7e-20
ABS74201.1 YngI BGC0001095 NRP 22.0 89.6 91.0 7.2e-18
AAL33757.1 putative_non-ribosomal_peptide_synthetase BGC0000421 NRP 22.0 94.1 86.0 1.4e-16
AGY30675.1 Ann3 BGC0001298 Polyketide 24.0 61.6 86.0 1.4e-16
AHI59110.1 locillomycin_synthase_C BGC0001005 NRP + Polyketide 22.0 100.6 86.0 1.8e-16
ABV79985.1 ApnA BGC0000301 NRP 22.0 102.8 84.0 6.7e-16
CAJ76290.1 putative_non-ribosomal_peptide_synthase BGC0000972 NRP + Polyketide:Modular type I + Polyketide:Trans-AT type I 23.0 77.8 83.0 1.2e-15
BAH43766.1 tyrocidine_synthetase_III BGC0000452 NRP 21.0 77.1 77.0 6.3e-14
AUO16404.1 4-guanidinobutanoate:CoA_ligase BGC0001700 Polyketide 21.0 80.9 75.0 3.1e-13
CZT62785.1 Non-ribosomal_peptide_synthase,_involved_in_Hassallidin_biosynthesis BGC0001614 NRP 23.0 97.2 74.0 9.1e-13
ARM20285.1 4-guanidinobutanoate:CoA_ligase BGC0001523 Polyketide 21.0 79.3 72.0 2.7e-12
AHD05614.1 putative_non-ribosomal_peptide_ligase/_polyketide_synthase_hybrid BGC0001033 NRP + Polyketide 21.0 77.2 71.0 4.5e-12
AEU11006.1 NpnB BGC0001029 NRP + Polyketide 23.0 100.2 70.0 1e-11
ACM79812.1 ZmaQ BGC0001059 NRP + Polyketide 22.0 77.4 70.0 1e-11
AAC06348.1 bacitracin_synthetase_3 BGC0000310 NRP 22.0 99.4 69.0 1.7e-11
APZ78846.1 nonribosomal_peptide_synthetase BGC0001431 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 81.8 67.0 8.5e-11
AHD05627.1 putative_non-ribosomal_peptide_ligase_domain_protein BGC0001033 NRP + Polyketide 22.0 96.4 66.0 1.5e-10
ARO38317.1 nonribosomal_peptide_synthetase BGC0001560 NRP + Polyketide 21.0 99.4 66.0 1.9e-10
CAE17553.1 acyl_CoA_ligase BGC0000210 Polyketide:Type II + Saccharide:Oligosaccharide 26.0 49.1 63.0 1.6e-09
CZT62784.1 Non-ribosomal_peptide_synthase,_involved_in_Hassallidin_biosynthesis BGC0001614 NRP 22.0 94.5 63.0 1.6e-09
AQX14497.1 monobactam_NRPS_scaffold_4 BGC0001672 NRP 23.0 45.6 62.0 2.1e-09
AAZ03550.1 McnA BGC0000332 NRP 22.0 101.3 61.0 8e-09
BAB69699.1 iturin_A_synthetase_B BGC0001098 NRP + Polyketide 23.0 44.8 61.0 8e-09
APZ78729.1 nonribosomal_peptide_synthetase BGC0001421 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 98.7 61.0 8e-09
APZ78744.1 nonribosomal_peptide_synthetase BGC0001422 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 78.8 60.0 1e-08
ATY37590.1 BogC BGC0001532 NRP 21.0 53.8 59.0 1.8e-08
ACZ55942.1 non-ribosomal_peptide_synthetase BGC0000302 NRP 21.0 100.0 59.0 2.3e-08
CAC17499.1 putative_non-ribosomal_peptide_synthase BGC0000324 NRP 23.0 51.9 59.0 3e-08
AGO86662.1 equisetin_synthetase BGC0001255 NRP + Polyketide 22.0 72.6 59.0 3e-08
CBF73453.1 nonribosomal_peptide_synthase,_putative_(JCVI) BGC0001515 NRP 21.0 95.1 59.0 3e-08
WP_013184596.1 non-ribosomal_peptide_synthetase BGC0001825 NRP 21.0 79.1 58.0 4e-08
CAO91861.1 PKS-NRPS_hybrid BGC0000968 NRP + Polyketide:Iterative type I 22.0 66.3 58.0 5.2e-08
CAG29032.1 nonribosomal_peptide_synthetase_(modules_3_to_6) BGC0001023 NRP + Polyketide:Modular type I 21.0 77.8 57.0 6.8e-08
APZ78822.1 nonribosomal_peptide_synthetase BGC0001429 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 77.8 57.0 6.8e-08
AQM58286.1 non-ribosomal_peptide_synthase BGC0001816 NRP + Polyketide 23.0 55.7 57.0 8.8e-08
WP_010369430.1 non-ribosomal_peptide_synthetase BGC0000314 Polyketide + NRP:Cyclic depsipeptide + Other:Aminocoumarin 22.0 79.0 57.0 1.2e-07
AAS47562.1 mixed_type_I_polyketide_synthase_-_peptide_synthetase BGC0001108 Polyketide:Trans-AT type I 22.0 77.7 56.0 1.5e-07
ctg1_orf8 BGC0001109 NRP + Polyketide 22.0 77.7 56.0 1.5e-07
APZ78692.1 nonribosomal_peptide_synthetase BGC0001418 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 77.8 56.0 2e-07
AXN93602.1 PuwF-G BGC0001952 NRP 22.0 99.7 56.0 2e-07
AXN93615.1 PuwG BGC0001953 NRP 21.0 96.7 56.0 2e-07
BAK26562.1 PKS-NRPS_hybrid BGC0000977 NRP + Polyketide 23.0 59.3 56.0 2.6e-07
APZ78782.1 nonribosomal_peptide_synthetase BGC0001426 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 79.0 54.0 5.7e-07
ACZ55944.1 non-ribosomal_peptide_synthetase BGC0000302 NRP 24.0 59.0 54.0 7.5e-07
AKD43499.1 Acyl-CoA_ligase BGC0001409 Polyketide 24.0 54.1 54.0 7.5e-07
AXN93614.1 PuwF BGC0001953 NRP 22.0 98.3 54.0 7.5e-07
APZ78704.1 nonribosomal_peptide_synthetase BGC0001419 NRP:Cyclic depsipeptide + Polyketide:Iterative type I 21.0 77.8 54.0 9.8e-07
AFH75329.1 nonribosomal_peptide_synthetase BGC0000398 NRP:Cyclic depsipeptide 21.0 53.3 53.0 1.7e-06
ATQ39428.1 cyclosporin_C_synthetase BGC0001565 NRP 22.0 55.5 52.0 2.2e-06
ctg1_orf10 BGC0000321 NRP 23.0 56.0 52.0 2.8e-06
CAA07759.1 acyl_CoA_ligase BGC0000246 Polyketide 22.0 62.7 51.0 4.8e-06
CAK50779.1 acyl_CoA_ligase BGC0000247 Polyketide:Type II + Saccharide:Oligosaccharide 22.0 62.7 51.0 4.8e-06
ADC79614.1 BafY BGC0000028 Polyketide:Modular type I 25.0 49.8 50.0 8.3e-06