LOCUS       c00020_NZ_JAEF..        2115 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JAEF01000031.1 Ruminococcus flavefaciens ATCC 19208
            L870DRAFT_scaffold00020.20_C, whole genome shotgun sequence.
ACCESSION   NZ_JAEF01000031
VERSION     NZ_JAEF01000031
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 12:56:51
            Original ID  :: NZ_JAEF01000031.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2115
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2115
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:2114](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3..2114)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..2115
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..2115
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     CDS             complement(3..2114)
                     /NRPS_PKS="Domain: Condensation_LCL (48-332). E-value:
                     7.3e-72. Score: 233.7. Matches aSDomain:
                     nrpspksdomains_ctg20_1_Condensation_LCL.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     264.7; E-value: 3.1e-80)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg20_1"
                     /sec_met_domain="Condensation (E-value: 1.6e-68, bitscore:
                     217.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.9e-47, bitscore:
                     146.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LVNRIEAETGVRIALKEVFAHPTAEQLAVLAESVGKEYGIIGKAE
                     NKEYYTATYQQEQIYITSVFLAESTQYNMPFAVKLNEGIDIERFKECIRKVVERNDVFR
                     YSFDIIENKVVFRINDVTALNIEEQTLDKEENYNKKIFEDFIRPFDLKSAPLIRAKIIS
                     FENSKRHIFMMDMHHIIADGLSIAIIFESVFALYNGIEYAETVPFVDYSQYLLQQDMSS
                     SEEYWVNEFSDELPEYEIVADYPRPAVMTRESKTCTFKFDSEMSRNIYKLCKAENVTPY
                     MFMLSTLIILLGKKSNSEDIVVGTPVSGRLDLSTEKTIGLFVNTLPIRVKFDKKDSFRD
                     ILMNVREKSIAAFDNQMYPLEKLLKKLDIKKESSHNPLFDIMFSMNNFELNRDDLERHG
                     VSIIEDNTLSVAKFDLFFNAHEDENNEFVISVEYCSELYRDGMMQAMIEQWAEIIRMYC
                     SDQNITVEKTQLPISNSVISQCAVEEEFEIKETVIEMLESSVKRNSDSVALVYEDENTT
                     YTKFSERVNIIANYLTMKGFGKGDCIAVMMPRCRELIEVIYAVMKAGCVYVPVSLDYPK
                     NRQDHIFNDCDVKAIITVNNPANEEPLSADRQVLLYDQLFEANDSSDNGCMCSLNDLAY
                     IIYTSGSSGNPKGVKISHGALTNIVLNLEYLHPTDKVIYLLKTSNMFDVSISEIFGWFV
                     SGGSLVILGP"
     PFAM_domain     complement(9..626)
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg20_1_0002"
                     /evalue="1.20E-45"
                     /label="ctg20_1"
                     /locus_tag="ctg20_1"
                     /protein_end="702"
                     /protein_start="496"
                     /score="155.9"
                     /tool="antismash"
                     /translation="ESSVKRNSDSVALVYEDENTTYTKFSERVNIIANYLTMKGFGKGD
                     CIAVMMPRCRELIEVIYAVMKAGCVYVPVSLDYPKNRQDHIFNDCDVKAIITVNNPANE
                     EPLSADRQVLLYDQLFEANDSSDNGCMCSLNDLAYIIYTSGSSGNPKGVKISHGALTNI
                     VLNLEYLHPTDKVIYLLKTSNMFDVSISEIFGWFVSGGSLVIL"
     CDS_motif       complement(156..215)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0006"
                     /evalue="4.90E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg20_1"
                     /protein_end="653"
                     /protein_start="633"
                     /score="28.3"
                     /tool="antismash"
                     /translation="AYIIYTSGSSGNPKGVKISH"
     CDS_motif       complement(405..440)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0005"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg20_1"
                     /protein_end="570"
                     /protein_start="558"
                     /score="14.9"
                     /tool="antismash"
                     /translation="AVMKAGCVYVPV"
     PFAM_domain     complement(705..1973)
                     /aSDomain="Condensation"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00668.22"
                     /description="Condensation domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg20_1_0001"
                     /evalue="2.40E-86"
                     /label="ctg20_1"
                     /locus_tag="ctg20_1"
                     /protein_end="470"
                     /protein_start="47"
                     /score="290.4"
                     /tool="antismash"
                     /translation="EYYTATYQQEQIYITSVFLAESTQYNMPFAVKLNEGIDIERFKEC
                     IRKVVERNDVFRYSFDIIENKVVFRINDVTALNIEEQTLDKEENYNKKIFEDFIRPFDL
                     KSAPLIRAKIISFENSKRHIFMMDMHHIIADGLSIAIIFESVFALYNGIEYAETVPFVD
                     YSQYLLQQDMSSSEEYWVNEFSDELPEYEIVADYPRPAVMTRESKTCTFKFDSEMSRNI
                     YKLCKAENVTPYMFMLSTLIILLGKKSNSEDIVVGTPVSGRLDLSTEKTIGLFVNTLPI
                     RVKFDKKDSFRDILMNVREKSIAAFDNQMYPLEKLLKKLDIKKESSHNPLFDIMFSMNN
                     FELNRDDLERHGVSIIEDNTLSVAKFDLFFNAHEDENNEFVISVEYCSELYRDGMMQAM
                     IEQWAEIIRMYCSDQNITVEKTQL"
     CDS_motif       complement(960..1079)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0004"
                     /evalue="6.60E-12"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="ctg20_1"
                     /protein_end="385"
                     /protein_start="345"
                     /score="37.7"
                     /tool="antismash"
                     /translation="REKSIAAFDNQMYPLEKLLKKLDIKKESSHNPLFDIMFSM"
     aSDomain        complement(1119..1970)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg20_1_Condensation_LCL.1"
                     /domain_subtype="Condensation_LCL"
                     /evalue="7.30E-72"
                     /label="ctg20_1_Condensation_LCL.1"
                     /locus_tag="ctg20_1"
                     /protein_end="332"
                     /protein_start="48"
                     /score="233.7"
                     /tool="antismash"
                     /translation="YYTATYQQEQIYITSVFLAESTQYNMPFAVKLNEGIDIERFKECI
                     RKVVERNDVFRYSFDIIENKVVFRINDVTALNIEEQTLDKEENYNKKIFEDFIRPFDLK
                     SAPLIRAKIISFENSKRHIFMMDMHHIIADGLSIAIIFESVFALYNGIEYAETVPFVDY
                     SQYLLQQDMSSSEEYWVNEFSDELPEYEIVADYPRPAVMTRESKTCTFKFDSEMSRNIY
                     KLCKAENVTPYMFMLSTLIILLGKKSNSEDIVVGTPVSGRLDLSTEKTIGLFVNTLPIR
                     VKF"
     CDS_motif       complement(1128..1217)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0003"
                     /evalue="2.40E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="ctg20_1"
                     /protein_end="329"
                     /protein_start="299"
                     /score="45.6"
                     /tool="antismash"
                     /translation="DIVVGTPVSGRLDLSTEKTIGLFVNTLPIR"
     CDS_motif       complement(1524..1589)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0002"
                     /evalue="2.40E-07"
                     /label="C3_LCL_132-143"
                     /locus_tag="ctg20_1"
                     /protein_end="197"
                     /protein_start="175"
                     /score="23.3"
                     /tool="antismash"
                     /translation="MHHIIADGLSIAIIFESVFALY"
     CDS_motif       complement(1794..1907)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0001"
                     /evalue="8.40E-11"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg20_1"
                     /protein_end="107"
                     /protein_start="69"
                     /score="34.3"
                     /tool="antismash"
                     /translation="TQYNMPFAVKLNEGIDIERFKECIRKVVERNDVFRYSF"
ORIGIN
        1 tcaggtccta atattaccaa tgatccgccg cttacgaacc atccgaaaat ctcggatatg
       61 gaaacatcaa acatattgct tgtctttaac aggtatatta ccttgtctgt cggatgcaga
      121 tactcaagat tcagaacgat atttgtcaga gcaccgtgag atatcttaac acccttagga
      181 tttccggatg aacctgaagt gtagataata tacgcaagat cattcagact gcacatacag
      241 ccattgtctg agctgtcatt tgcttcaaac agctgatcgt aaagcagtac ctgtcggtcg
      301 gcactcaacg gctcttcatt agctggatta tttacagtga ttattgcctt gacatcacag
      361 tcgttgaata tatgatcctg tctgttttta ggatagtcaa gacttacagg cacataaaca
      421 cagcctgcct tcattacagc atagataact tctatcagtt cacggcatct tggcatcatt
      481 actgcaatgc aatcaccctt tccgaatccc ttcatcgtaa ggtaatttgc tatgatatta
      541 actctttcag aaaacttggt atatgttgta ttctcgtctt catataccaa agcaacactg
      601 tcgctgtttc tcttgactga gctttcaagc atttcgataa ctgtttcttt gatctcaaat
      661 tcctcttcaa cagcacactg tgaaataaca gagttgctta tcggcagctg agtcttttct
      721 acagtgatat tctgatcgct gcaatacatt ctgataattt cagcccactg ttctatcatt
      781 gcctgcatca taccgtctct gtataattca ctgcaatact ctacagaaat aacaaattca
      841 ttgttttcat cttcgtgggc attaaagaac agatcaaact tagccacaga caatgtatta
      901 tcttctatta ttgatacacc gtgtctttca agatcgtctc tgttcagctc aaagttattc
      961 attgagaaca tgatatcaaa caacggatta tgagatgatt cctttttaat atcaagcttc
     1021 ttcagtaact tttccaaagg atacatctga ttgtcaaatg cagctatgct tttttctctg
     1081 acattcatta atatatccct gaaactgtcc tttttatcaa acttcactct gatcggcaaa
     1141 gtatttacaa agagtcctat cgttttttcg gtactcaggt ctaatctgcc cgatacagga
     1201 gtgcccacaa ctatatcttc tgaatttgat ttctttccga gaagtattat aagtgtagac
     1261 agcatgaaca tatacggtgt tacattttct gccttgcaca gcttataaat attacggctc
     1321 atttcactgt caaatttgaa tgtacaggtc ttgctctccc ttgtcataac cgcaggtctc
     1381 ggataatcag ccactatttc atattcagga agctcatcgg aaaactcatt tacccagtat
     1441 tcttcactgc tgctcatatc ctgctgaagc aaatactgcg aatagtcaac gaacggaact
     1501 gtttctgcgt attcaattcc gttatacaat gcgaaaacag attcaaatat aatagctatt
     1561 gataatccgt cagctatgat atgatgcata tccatcataa aaatatgtct tttgctgttt
     1621 tcaaagctta ttatcttggc tctgatcaac ggtgcagatt taaggtcaaa cggtcttata
     1681 aagtcttcaa atatcttttt attatagttt tcttctttat caagagtctg ctcttcaata
     1741 ttcaaagcag tgacatcatt tatcctgaat actaccttgt tttcaataat atcaaacgaa
     1801 tatctgaata catcgttcct ttctacaact tttcttatgc attccttgaa cctttctata
     1861 tctatacctt cattcagctt cactgcaaac ggcatattat actgggtcga ttctgccaga
     1921 aataccgaag taatatatat ctgctcctgc tgatatgtag ctgtataata ttctttattt
     1981 tcagcttttc ctattattcc gtattcttta ccgacacttt ctgcaagaac tgcaagctgt
     2041 tctgctgtag ggtgtgcaaa tacttccttc aatgctattc ttacgcctgt ttctgcttct
     2101 attctgttca caagc
//
