LOCUS       c00023_NZ_AOFM..        1776 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_AOFM01000025.1 Bacillus sonorensis L12 contig25, whole genome
            shotgun sequence.
ACCESSION   NZ_AOFM01000025
VERSION     NZ_AOFM01000025
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 16:22:17
            Original ID  :: NZ_AOFM01000025.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1776
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1776
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1:1774](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2..1774
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..1776
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1776
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             2..1774
                     /NRPS_PKS="Domain: AMP-binding (49-435). E-value: 1.1e-108.
                     Score: 355.3. Matches aSDomain:
                     nrpspksdomains_ctg23_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (541-591). E-value: 1.8e-19. Score:
                     61.6. Matches aSDomain: nrpspksdomains_ctg23_1_PCP.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     365.5; E-value: 6.1e-111)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg23_1"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-120, bitscore:
                     387.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.2e-12, bitscore:
                     32.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LNLLEAVAQNPNVPLSDLSLLNEAEKRRIVEVWNETELEVPADKT
                     IHELFEAQVNRTPDHGAAVYNGRRWTYRELNARANRLARLLIEKGARPEQRIGIMLKPS
                     LDMASGVLAVLKAGAAYVPIDPGYPEQRIKYVLEDSGAELLLTQTGVAVPDGFCGEAIL
                     LDSVLSGDVSPADDVNPQSGAEPHNLAYLIYTSGTTGQPKGVMVEHQSLVNLCYWHNDA
                     FNVTEHDKSAKYAGFGFDASVWEMFPYWIAGAELHIIDEAIRKDIVRLNRYFEEHGITI
                     TFLPTQLCEQFMELDNRSLRVLLTGGDRLKRVEKRGYSLVNNYGPTENTVVATSGAIDP
                     DEGMLSIGKPIANTRVYVLGEHNQVQPAGVTGELCIAGRGLARGYLNKPEETEKRFTED
                     PFAPGERMYRTGDAVRWLPDGRIEYIGRIDQQVNIRGFRIELSEIEVQLAKLPGVQEAV
                     VTDIEDASGNKALCGYVTSAERLDTESLAGQVAKTLPDYMVPAYWVQLDELPMTANGKV
                     DKRALPQPDLKAQTAEYKAPRTDIEQLLADIWQEVLGIGRIGISDNFFALGGDSIKGIQ
                     MASRLQQHGWKLEMK"
     aSDomain        149..1306
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg23_1_AMP-binding.1"
                     /evalue="1.10E-108"
                     /label="ctg23_1_AMP-binding.1"
                     /locus_tag="ctg23_1"
                     /protein_end="435"
                     /protein_start="49"
                     /score="355.3"
                     /specificity="consensus: leu"
                     /tool="antismash"
                     /translation="FEAQVNRTPDHGAAVYNGRRWTYRELNARANRLARLLIEKGARPE
                     QRIGIMLKPSLDMASGVLAVLKAGAAYVPIDPGYPEQRIKYVLEDSGAELLLTQTGVAV
                     PDGFCGEAILLDSVLSGDVSPADDVNPQSGAEPHNLAYLIYTSGTTGQPKGVMVEHQSL
                     VNLCYWHNDAFNVTEHDKSAKYAGFGFDASVWEMFPYWIAGAELHIIDEAIRKDIVRLN
                     RYFEEHGITITFLPTQLCEQFMELDNRSLRVLLTGGDRLKRVEKRGYSLVNNYGPTENT
                     VVATSGAIDPDEGMLSIGKPIANTRVYVLGEHNQVQPAGVTGELCIAGRGLARGYLNKP
                     EETEKRFTEDPFAPGERMYRTGDAVRWLPDGRIEYIGRIDQQVNIR"
     PFAM_domain     149..1306
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_1_0001"
                     /evalue="4.80E-99"
                     /label="ctg23_1"
                     /locus_tag="ctg23_1"
                     /protein_end="435"
                     /protein_start="49"
                     /score="331.9"
                     /tool="antismash"
                     /translation="FEAQVNRTPDHGAAVYNGRRWTYRELNARANRLARLLIEKGARPE
                     QRIGIMLKPSLDMASGVLAVLKAGAAYVPIDPGYPEQRIKYVLEDSGAELLLTQTGVAV
                     PDGFCGEAILLDSVLSGDVSPADDVNPQSGAEPHNLAYLIYTSGTTGQPKGVMVEHQSL
                     VNLCYWHNDAFNVTEHDKSAKYAGFGFDASVWEMFPYWIAGAELHIIDEAIRKDIVRLN
                     RYFEEHGITITFLPTQLCEQFMELDNRSLRVLLTGGDRLKRVEKRGYSLVNNYGPTENT
                     VVATSGAIDPDEGMLSIGKPIANTRVYVLGEHNQVQPAGVTGELCIAGRGLARGYLNKP
                     EETEKRFTEDPFAPGERMYRTGDAVRWLPDGRIEYIGRIDQQVNIR"
     aSModule        149..1774
                     /complete
                     /domains="nrpspksdomains_ctg23_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg23_1_PCP.1"
                     /locus_tags="ctg23_1"
                     /monomer_pairings="leu -> leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     aSDomain        212..1378
                     /aSDomain="TIGR01733"
                     /aSTool="tigrfam"
                     /description="AA-adenyl-dom: amino acid adenylation domain"
                     /detection="hmmscan"
                     /domain_id="TIGRFam_ctg23_1_0001"
                     /evalue="1.00E-129"
                     /identifier="TIGR01733"
                     /label="ctg23_1"
                     /locus_tag="ctg23_1"
                     /protein_end="459"
                     /protein_start="70"
                     /score="430.9"
                     /tool="antismash"
                     /translation="TYRELNARANRLARLLIEKGARPEQRIGIMLKPSLDMASGVLAVL
                     KAGAAYVPIDPGYPEQRIKYVLEDSGAELLLTQTGVAVPDGFCGEAILLDSVLSGDVSP
                     ADDVNPQSGAEPHNLAYLIYTSGTTGQPKGVMVEHQSLVNLCYWHNDAFNVTEHDKSAK
                     YAGFGFDASVWEMFPYWIAGAELHIIDEAIRKDIVRLNRYFEEHGITITFLPTQLCEQF
                     MELDNRSLRVLLTGGDRLKRVEKRGYSLVNNYGPTENTVVATSGAIDPDEGMLSIGKPI
                     ANTRVYVLGEHNQVQPAGVTGELCIAGRGLARGYLNKPEETEKRFTEDPFAPGERMYRT
                     GDAVRWLPDGRIEYIGRIDQQVNIRGFRIELSEIEVQLAKLPGVQEAVV"
     CDS_motif       335..376
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg23_1_0001"
                     /evalue="1.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg23_1"
                     /protein_end="125"
                     /protein_start="111"
                     /score="21.6"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPID"
     CDS_motif       569..628
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg23_1_0002"
                     /evalue="2.90E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg23_1"
                     /protein_end="209"
                     /protein_start="189"
                     /score="35.0"
                     /tool="antismash"
                     /translation="AYLIYTSGTTGQPKGVMVEH"
     CDS_motif       968..997
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg23_1_0003"
                     /evalue="3.10E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg23_1"
                     /protein_end="332"
                     /protein_start="322"
                     /score="10.2"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
     CDS_motif       1100..1189
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg23_1_0004"
                     /evalue="2.50E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg23_1"
                     /protein_end="396"
                     /protein_start="366"
                     /score="51.7"
                     /tool="antismash"
                     /translation="PAGVTGELCIAGRGLARGYLNKPEETEKRF"
     CDS_motif       1271..1336
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg23_1_0005"
                     /evalue="6.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg23_1"
                     /protein_end="445"
                     /protein_start="423"
                     /score="34.3"
                     /tool="antismash"
                     /translation="YIGRIDQQVNIRGFRIELSEIE"
     PFAM_domain     1328..1549
                     /aSDomain="AMP-binding_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13193.8"
                     /description="AMP-binding enzyme C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_1_0002"
                     /evalue="3.20E-11"
                     /label="ctg23_1"
                     /locus_tag="ctg23_1"
                     /protein_end="516"
                     /protein_start="442"
                     /score="44.1"
                     /tool="antismash"
                     /translation="EIEVQLAKLPGVQEAVVTDIEDASGNKALCGYVTSAERLDTESLA
                     GQVAKTLPDYMVPAYWVQLDELPMTANGK"
     aSDomain        1625..1774
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg23_1_PCP.1"
                     /evalue="1.80E-19"
                     /label="ctg23_1_PCP.1"
                     /locus_tag="ctg23_1"
                     /protein_end="591"
                     /protein_start="541"
                     /score="61.6"
                     /tool="antismash"
                     /translation="EQLLADIWQEVLGIGRIGISDNFFALGGDSIKGIQMASRLQQHGW
                     KLEMK"
     PFAM_domain     1631..1768
                     /aSDomain="PP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00550.27"
                     /description="Phosphopantetheine attachment site"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg23_1_0003"
                     /evalue="2.60E-08"
                     /label="ctg23_1"
                     /locus_tag="ctg23_1"
                     /protein_end="589"
                     /protein_start="543"
                     /score="34.2"
                     /tool="antismash"
                     /translation="LLADIWQEVLGIGRIGISDNFFALGGDSIKGIQMASRLQQHGWKL
                     E"
ORIGIN
        1 gctcaatctg ctcgaagcgg ttgcgcaaaa cccgaatgtc cccctttcag acctttccct
       61 gctcaatgaa gcggaaaaac gccgcatcgt tgaagtgtgg aatgagacgg agctggaagt
      121 gccggcggac aaaacgattc atgagctgtt tgaagcgcag gtaaaccgca ctcctgacca
      181 cggggcagcg gtgtacaacg gccgccgctg gacgtacagg gagctcaacg cccgcgcgaa
      241 ccgcctggcc cgcctgctca tcgaaaaagg tgcgcgtccg gaacagcgga tcggcatcat
      301 gcttaagccg tcccttgaca tggcgtccgg cgtgctggcg gttttaaaag cgggagcggc
      361 atatgtgccg atcgaccctg gctatccgga acaaagaatc aaatatgttt tggaagacag
      421 cggggcggag ctcctgctga cgcaaacggg tgtagccgtg cctgacggct tctgcgggga
      481 agcgattcta ctcgattctg tactgagcgg ggatgtatcg cctgcagacg atgtcaaccc
      541 tcagtccgga gctgagcctc acaatctcgc gtacttaatc tacacatcag gcacgacggg
      601 ccagccgaaa ggcgtcatgg tcgagcacca gtcgctcgta aatttgtgct attggcacaa
      661 tgacgcgttc aacgtcacag aacacgacaa gagcgcgaaa tacgccggct tcggctttga
      721 cgcatctgtg tgggaaatgt tcccgtattg gatagccgga gccgagctgc atatcatcga
      781 tgaagcgatt cggaaggaca tcgtccgcct gaatcgatat ttcgaagagc acggcatcac
      841 gatcacattc ctgccgacac agctgtgcga gcagtttatg gagctggaca accggtcact
      901 gcgcgtgctt ctgacgggcg gtgacaggct gaaacgggtg gaaaagcgcg gctattcgct
      961 cgtcaacaac tacggtccga cggaaaacac ggtcgtcgcc acaagcggtg cgattgatcc
     1021 tgatgaagga atgctttcga tcggcaagcc gattgccaac acgcgcgtct atgtgctcgg
     1081 agagcacaat caagtccagc ctgccggtgt gacgggtgaa ctgtgcatcg ccggacgggg
     1141 gcttgcgcgc ggctacctca acaaaccgga ggaaacggag aagcgtttta cagaggaccc
     1201 gttcgcaccg ggcgagcgga tgtacaggac gggtgatgcg gtcaggtggc ttccggacgg
     1261 ccggatcgaa tatatcggcc ggatcgacca gcaggtcaac atccgcggct tccggatcga
     1321 gctttcagaa atcgaagtcc agctggccaa gcttccgggc gtacaggaag cggtcgtcac
     1381 tgacattgag gacgcctccg gcaacaaagc gctttgcggc tatgtcacat cagctgaaag
     1441 gcttgacaca gaaagccttg ccggccaggt ggcgaagacg ctgccggact acatggtgcc
     1501 cgcttattgg gtgcagctcg atgagctgcc gatgacggcg aacggaaaag tcgataaacg
     1561 ggcgcttccg cagcctgacc taaaagcgca gacagctgaa tacaaagcgc cgagaacgga
     1621 tattgaacag ctgcttgccg atatttggca ggaggttctc ggcattggcc gaatcggaat
     1681 cagcgacaat ttctttgcgc tcggcgggga ctcgatcaaa gggattcaaa tggcgagccg
     1741 ccttcagcag cacggctgga agcttgaaat gaaaga
//
