GCF_003667725.1_ASM366772v1_genomic - 6 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP033022.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	terpene	1,242,458	1,263,303

NZ_CP033023.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NAPAA	59,813	93,847
Region 2.2	thioamitides	613,615	635,524
Region 2.3	NRPS,T1PKS	698,220	777,542	tubulysin A	NRP + Polyketide	6%

NZ_CP033025.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	hserlactone	136,621	147,633

NZ_CP033026.1

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	hserlactone	166,219	177,577

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	terpene	1,242,458	1,263,303
Region 2.1	NAPAA	59,813	93,847
Region 2.2	thioamitides	613,615	635,524
Region 2.3	NRPS,T1PKS	698,220	777,542	tubulysin A	NRP + Polyketide	6%
Region 4.1	hserlactone	136,621	147,633
Region 5.1	hserlactone	166,219	177,577

No secondary metabolite regions were found in these records:: NZ_CP033024.1

Compact view

NZ_CP033022.1 - Region 1 - terpene

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Location: 1,242,458 - 1,263,303 nt. (total: 20,846 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000660.1	0.24	Terpene	albaflavenone	Streptomyces coelicolor A3(2)
BGC0000674.1	0.23	Terpene	(-)-δ-cadinene	Streptomyces clavuligerus ATCC 27064
BGC0000678.1	0.21	Terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000286.1	0.21	Polyketide	viguiepinol	Streptomyces sp. KO-3988
BGC0000675.1	0.20	Terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001336.1	0.20	Alkaloid	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000823.1	0.20	Alkaloid	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001223.1	0.19	Other	hydroxysporine	uncultured bacterium
BGC0001595.1	0.18	Alkaloid	fischerindole	Fischerella sp. SAG 46.79
BGC0001225.1	0.18	NRP	celesticetin	Streptomyces caelestis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000660.1	0.57	Terpene	albaflavenone	Streptomyces coelicolor A3(2)
BGC0000674.1	0.56	Terpene	(-)-δ-cadinene	Streptomyces clavuligerus ATCC 27064
BGC0000678.1	0.54	Terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000286.1	0.53	Polyketide	viguiepinol	Streptomyces sp. KO-3988
BGC0000675.1	0.53	Terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001336.1	0.52	Alkaloid	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000823.1	0.52	Alkaloid	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001223.1	0.51	Other	hydroxysporine	uncultured bacterium
BGC0001595.1	0.50	Alkaloid	fischerindole	Fischerella sp. SAG 46.79
BGC0001225.1	0.50	NRP	celesticetin	Streptomyces caelestis

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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NZ_CP033023.1 - Region 1 - NAPAA

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Location: 59,813 - 93,847 nt. (total: 34,035 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and PP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Pfam domains

TIGRFAM domains

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000410.1	0.23	NRP	pseudomonine	Pseudomonas fluorescens
BGC0000375.1	0.22	NRP	indigoidine	Streptomyces chromofuscus
BGC0000427.1	0.21	NRP	shinorine, 4-deoxygadusol, mycosporine glycine	Trichormus variabilis ATCC 29413
BGC0001399.1	0.21	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000888.1	0.21	Other	bacilysin	Bacillus sp. CS93
BGC0001184.1	0.21	Other	bacilysin	Bacillus velezensis FZB42
BGC0001197.1	0.20	Polyketide	frankiamicin	Frankia sp. EAN1pec
BGC0000228.1	0.19	Polyketide	granaticin	Streptomyces violaceoruber
BGC0001758.1	0.18	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001211.1	0.18	NRP	albachelin	Amycolatopsis alba DSM 44262

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000410.1	0.56	NRP	pseudomonine	Pseudomonas fluorescens
BGC0000375.1	0.55	NRP	indigoidine	Streptomyces chromofuscus
BGC0000427.1	0.54	NRP	shinorine, 4-deoxygadusol, mycosporine glycine	Trichormus variabilis ATCC 29413
BGC0001399.1	0.54	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000888.1	0.54	Other	bacilysin	Bacillus sp. CS93
BGC0001184.1	0.54	Other	bacilysin	Bacillus velezensis FZB42
BGC0001197.1	0.53	Polyketide	frankiamicin	Frankia sp. EAN1pec
BGC0000228.1	0.51	Polyketide	granaticin	Streptomyces violaceoruber
BGC0001758.1	0.50	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001211.1	0.50	NRP	albachelin	Amycolatopsis alba DSM 44262

Similar known gene clusters

No matches found.

Module view

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Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 59812 - 93847:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_65

Search NORINE for peptide: strict or relaxed

AMP-binding (45..446): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DmEHNgTVtK
Nearest Stachelhaus code:: dab
Stachelhaus code match:: 70% (weak)

NZ_CP033023.1 - Region 2 - thioamitides

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Location: 613,615 - 635,524 nt. (total: 21,910 nt) Show pHMM detection rules used

thioamitides: ((YcaO or TIGR03882) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.1	0.26	RiPP	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001526.1	0.21	Other	bartolosides E, bartolosides F, bartolosides G, bartolosides H, bartolosides I, bartolosides J, bartolosides K	Synechocystis salina LEGE 06099
BGC0001525.1	0.17	Other	bartoloside 2, bartoloside 3, bartoloside 4	Synechocystis salina LEGE 06155
BGC0000554.1	0.16	RiPP	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000538.1	0.16	RiPP	nisin Z	Lactococcus lactis
BGC0000571.1	0.16	RiPP	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0000541.1	0.16	RiPP	paenicidin A	Paenibacillus polymyxa
BGC0000560.1	0.16	RiPP	subtilomycin	Bacillus subtilis
BGC0001173.1	0.16	RiPP	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000552.1	0.16	RiPP	SmbA, SmbB	Streptococcus mutans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.1	0.60	RiPP	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001526.1	0.53	Other	bartolosides E, bartolosides F, bartolosides G, bartolosides H, bartolosides I, bartolosides J, bartolosides K	Synechocystis salina LEGE 06099
BGC0001525.1	0.48	Other	bartoloside 2, bartoloside 3, bartoloside 4	Synechocystis salina LEGE 06155
BGC0000554.1	0.47	RiPP	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000538.1	0.47	RiPP	nisin Z	Lactococcus lactis
BGC0000571.1	0.47	RiPP	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0000541.1	0.47	RiPP	paenicidin A	Paenibacillus polymyxa
BGC0000560.1	0.46	RiPP	subtilomycin	Bacillus subtilis
BGC0001173.1	0.46	RiPP	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000552.1	0.46	RiPP	SmbA, SmbB	Streptococcus mutans

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

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Detailed TIGRFAM domain annotation

Selected features only

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NZ_CP033023.1 - Region 3 - NRPS,T1PKS

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Location: 698,220 - 777,542 nt. (total: 79,323 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Pfam domains

TIGRFAM domains

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000457.1	0.65	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001434.1	0.65	NRP	nematophin	Xenorhabdus budapestensis
BGC0001698.1	0.65	NRP	nevaltophin A, nevaltophin B, nevaltophin C, nevaltophin D	Xenorhabdus budapestensis
BGC0001748.1	0.62	NRP, Polyketide	pseudospumigin A, pseudospumigin B, pseudospumigin C, pseudospumigin D, pseudospumigin E, pseudospumigin F	Nostoc sp. CENA543
BGC0001833.1	0.62	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001050.1	0.62	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000373.1	0.62	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0001399.1	0.62	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000347.1	0.61	NRP	equibactin	Streptococcus equi subsp. equi
BGC0000426.1	0.61	NRP	sevadicin	Paenibacillus larvae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000347.1	0.66	NRP	equibactin	Streptococcus equi subsp. equi
BGC0000457.1	0.66	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001434.1	0.66	NRP	nematophin	Xenorhabdus budapestensis
BGC0001698.1	0.66	NRP	nevaltophin A, nevaltophin B, nevaltophin C, nevaltophin D	Xenorhabdus budapestensis
BGC0001748.1	0.65	NRP, Polyketide	pseudospumigin A, pseudospumigin B, pseudospumigin C, pseudospumigin D, pseudospumigin E, pseudospumigin F	Nostoc sp. CENA543
BGC0001833.1	0.65	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001050.1	0.64	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000373.1	0.64	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0001399.1	0.64	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000426.1	0.64	NRP	sevadicin	Paenibacillus larvae

Similar known gene clusters

Module view

Legend

Detailed Pfam domain annotation

Selected features only

Expand to show all names

Detailed TIGRFAM domain annotation

Selected features only

Expand to show all names

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 698219 - 777542:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly) + (ohmal) + (X) + (ohmal) + (ala - cys) + (gly - D-ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_644

Search NORINE for peptide: strict or relaxed

AMP-binding (519..911): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg2_646

PKS_AT (568..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.2
inactive: 90.3
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 43.8
2-Methylbutyryl-CoA: 28.3
Ethylmalonyl-CoA: 27.9
CHC-CoA: 21.4
Benzoyl-CoA: 20.8
fatty_acid: 17.9
Isobutyryl-CoA: 17.6
trans-1,2-CPDA: 17.2
Acetyl-CoA: 11.3
3-Methylbutyryl-CoA: 10.4

ctg2_647

CAL_domain (173..507): pk

minowa: NH2

Prediction, score:

NH2: 101.6
Acetyl-CoA: 40.9
fatty_acid: 12.6
AHBA: 12.4
shikimic_acid: 0.0

ctg2_648

Search NORINE for peptide: strict or relaxed

AMP-binding (537..929): X

NRPSPredictor2: tyr, bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DApTLGAvnK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)

ctg2_654

PKS_AT (537..824): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 156.9
inactive: 92.6
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 53.8
Propionyl-CoA: 39.6
2-Methylbutyryl-CoA: 24.4
CHC-CoA: 21.9
Benzoyl-CoA: 21.4
Isobutyryl-CoA: 19.5
Ethylmalonyl-CoA: 15.4
3-Methylbutyryl-CoA: 14.3
trans-1,2-CPDA: 12.6
Acetyl-CoA: 11.9
fatty_acid: 8.7

ctg2_655

Search NORINE for peptide: strict or relaxed

AMP-binding (508..895): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

AMP-binding (1551..1948): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLyNMSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

ctg2_656

Search NORINE for peptide: strict or relaxed

AMP-binding (490..888): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1543..1930): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNcALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 90% (moderate)

NZ_CP033025.1 - Region 1 - hserlactone

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Location: 136,621 - 147,633 nt. (total: 11,013 nt) Show pHMM detection rules used

Region on contig edge.

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	hserlactone	Similarity score	Type	Compound(s)	Organism
BGC0000329.1		0.04	NRP	corpeptin A, corpeptin B	Pseudomonas corrugata

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000329.1		0.18	NRP	corpeptin A, corpeptin B	Pseudomonas corrugata

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

Expand to show all names

Detailed TIGRFAM domain annotation

Selected features only

Expand to show all names

NZ_CP033026.1 - Region 1 - hserlactone

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Location: 166,219 - 177,577 nt. (total: 11,359 nt) Show pHMM detection rules used

Region on contig edge.

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

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resistance

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Gene details

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MIBiG comparison

KnownClusterBlast

Pfam domains

TIGRFAM domains

Similar gene clusters

Analysis type:

Reference	hserlactone	Similarity score	Type	Compound(s)	Organism
BGC0000329.1		0.04	NRP	corpeptin A, corpeptin B	Pseudomonas corrugata

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000329.1		0.19	NRP	corpeptin A, corpeptin B	Pseudomonas corrugata

Similar known gene clusters

No matches found.

Detailed Pfam domain annotation

Selected features only

Expand to show all names

Detailed TIGRFAM domain annotation

Selected features only

Expand to show all names