LOCUS       c00046_NZ_JARF..        2354 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  NZ_JARFMK010000051.1 Bradyrhizobium yuanmingense strain ALSPC2061
            NODE_51_length_2354_cov_81.2048, whole genome shotgun sequence.
ACCESSION   c00046_NZ_JARF..
VERSION     c00046_NZ_JARF..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.1.1
            Run date     :: 2024-03-14 14:27:07
            Original ID  :: NZ_JARFMK010000051.1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2354
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     PFAM_domain     1..150
                     /aSDomain="AMP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00501.30"
                     /description="AMP-binding enzyme"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg46_1_0001"
                     /evalue="1.10E-12"
                     /label="ctg46_1"
                     /locus_tag="ctg46_1"
                     /protein_end="50"
                     /protein_start="0"
                     /score="47.4"
                     /tool="antismash"
                     /translation="LNRPELTAERFIASPFVDGDRLYRTGDLGRYLPDGNLEFLGRNDD
                     QVKIR"
     CDS             1..2352
                     /NRPS_PKS="Domain: PCP (166-233). E-value: 6.6e-28. Score:
                     88.6. Matches aSDomain: nrpspksdomains_ctg46_1_PCP.1"
                     /NRPS_PKS="Domain: Condensation_Dual (281-577). E-value:
                     1.1e-140. Score: 459.9. Matches aSDomain:
                     nrpspksdomains_ctg46_1_Condensation_Dual.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     185; E-value: 4.7e-56)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg46_1"
                     /sec_met_domain="Condensation (E-value: 4.5e-45, bitscore:
                     140.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.5e-30, bitscore:
                     90.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6e-18, bitscore:
                     51.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LNRPELTAERFIASPFVDGDRLYRTGDLGRYLPDGNLEFLGRNDD
                     QVKIRGFRIEPGEIAARLCEHALVREAVVVAQQDRRGDKHLVGYVVCAPEAQSDEVDGP
                     DLANTLRAHLSSRLPDYMVPSAFVRLEVLPLTVNGKLDRKALPPPADDAYARAAYEAPQ
                     GAVETALARIWAELLGLERVGRNDHFFELGGHSLLAVQVSSRLSQAVGVELPLSTLFAT
                     PVLADFAASLGRPHEVAVPASLITEQSTAITPQMLPLIELAQPEIDRIIATVPGGVRNI
                     QDIYALTPLQDGILFHHLLASRGDPYLLVSQMAFADRNLLERYLGAVQQVVDRHDILRT
                     AFVWEGLSSPAQVVWRRASLQVSEVELEGCDGCAADELRRRFDPRRQRIDLGRAPLLRF
                     VTARDPGSGRWLLLELQHHLIGDHTTLEVMHAEVRAMLAGRADELAAPLPFRNLVAQAR
                     LAVDAKAHEEFFRGLLADIDEPTMPFGLSEVYGDGSGVGEAHRMLPQALNERLREQARR
                     LGVSLASLCHLAWGQVVARSSGREQVVFGTVLFGRMHAGAGADRTMGLFINTLPVRLAL
                     DETGVAASVRSTHAQLSELLAHEHASLALAQRCSGVAAPAPLFSALLNYRHNRPAATPG
                     SGPDDVLSGVEWLGEEERTNYPVTLSVEDYGEALGLTAQVAAPVSADRLCGYMQRALEQ
                     LAQALEHAPNTPVRELDILPADERNYLLEELNRTAAAYPSERCIHELFEAQVQKAPDAM
                     AVVHEDERLSYGELNARANRLAHQLIALGVR"
     protocluster    1..2354
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:2352](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..2352
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..2354
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..2354
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     CDS_motif       115..177
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0001"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg46_1"
                     /protein_end="59"
                     /protein_start="38"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRNDDQVKIRGFRIEPGEI"
     PFAM_domain     172..420
                     /aSDomain="AMP-binding_C"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF13193.8"
                     /description="AMP-binding enzyme C-terminal domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg46_1_0002"
                     /evalue="3.90E-13"
                     /label="ctg46_1"
                     /locus_tag="ctg46_1"
                     /protein_end="140"
                     /protein_start="57"
                     /score="50.3"
                     /tool="antismash"
                     /translation="EIAARLCEHALVREAVVVAQQDRRGDKHLVGYVVCAPEAQSDEVD
                     GPDLANTLRAHLSSRLPDYMVPSAFVRLEVLPLTVNGK"
     CDS_motif       217..273
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0002"
                     /evalue="8.00E+00"
                     /label="C5_LCL_267-296"
                     /locus_tag="ctg46_1"
                     /protein_end="91"
                     /protein_start="72"
                     /score="-0.8"
                     /tool="antismash"
                     /translation="VVVAQQDRRGDKHLVGYVV"
     aSDomain        499..699
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg46_1_PCP.1"
                     /evalue="6.60E-28"
                     /label="ctg46_1_PCP.1"
                     /locus_tag="ctg46_1"
                     /protein_end="233"
                     /protein_start="166"
                     /score="88.6"
                     /tool="antismash"
                     /translation="ETALARIWAELLGLERVGRNDHFFELGGHSLLAVQVSSRLSQAVG
                     VELPLSTLFATPVLADFAASLG"
     PFAM_domain     505..690
                     /aSDomain="PP-binding"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00550.27"
                     /description="Phosphopantetheine attachment site"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg46_1_0003"
                     /evalue="1.50E-13"
                     /label="ctg46_1"
                     /locus_tag="ctg46_1"
                     /protein_end="230"
                     /protein_start="168"
                     /score="50.9"
                     /tool="antismash"
                     /translation="ALARIWAELLGLERVGRNDHFFELGGHSLLAVQVSSRLSQAVGVE
                     LPLSTLFATPVLADFAA"
     aSDomain        844..1731
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg46_1_Condensation_Dual.1"
                     /domain_subtype="Condensation_Dual"
                     /evalue="1.10E-140"
                     /label="ctg46_1_Condensation_Dual.1"
                     /locus_tag="ctg46_1"
                     /protein_end="577"
                     /protein_start="281"
                     /score="459.9"
                     /tool="antismash"
                     /translation="QDIYALTPLQDGILFHHLLASRGDPYLLVSQMAFADRNLLERYLG
                     AVQQVVDRHDILRTAFVWEGLSSPAQVVWRRASLQVSEVELEGCDGCAADELRRRFDPR
                     RQRIDLGRAPLLRFVTARDPGSGRWLLLELQHHLIGDHTTLEVMHAEVRAMLAGRADEL
                     AAPLPFRNLVAQARLAVDAKAHEEFFRGLLADIDEPTMPFGLSEVYGDGSGVGEAHRML
                     PQALNERLREQARRLGVSLASLCHLAWGQVVARSSGREQVVFGTVLFGRMHAGAGADRT
                     MGLFINTLPVRLALD"
     PFAM_domain     844..2163
                     /aSDomain="Condensation"
                     /aSTool="clusterhmmer"
                     /database="34.0"
                     /db_xref="PF00668.22"
                     /description="Condensation domain"
                     /detection="hmmscan"
                     /domain_id="clusterhmmer_ctg46_1_0004"
                     /evalue="8.90E-38"
                     /label="ctg46_1"
                     /locus_tag="ctg46_1"
                     /protein_end="721"
                     /protein_start="281"
                     /score="130.2"
                     /tool="antismash"
                     /translation="QDIYALTPLQDGILFHHLLASRGDPYLLVSQMAFADRNLLERYLG
                     AVQQVVDRHDILRTAFVWEGLSSPAQVVWRRASLQVSEVELEGCDGCAADELRRRFDPR
                     RQRIDLGRAPLLRFVTARDPGSGRWLLLELQHHLIGDHTTLEVMHAEVRAMLAGRADEL
                     AAPLPFRNLVAQARLAVDAKAHEEFFRGLLADIDEPTMPFGLSEVYGDGSGVGEAHRML
                     PQALNERLREQARRLGVSLASLCHLAWGQVVARSSGREQVVFGTVLFGRMHAGAGADRT
                     MGLFINTLPVRLALDETGVAASVRSTHAQLSELLAHEHASLALAQRCSGVAAPAPLFSA
                     LLNYRHNRPAATPGSGPDDVLSGVEWLGEEERTNYPVTLSVEDYGEALGLTAQVAAPVS
                     ADRLCGYMQRALEQLAQALEHAPNTPVRELDILPADERNYL"
     CDS_motif       853..894
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0003"
                     /evalue="9.10E-06"
                     /label="C1_dual_004-017"
                     /locus_tag="ctg46_1"
                     /protein_end="298"
                     /protein_start="284"
                     /score="18.2"
                     /tool="antismash"
                     /translation="YALTPLQDGILFHH"
     CDS_motif       919..1032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0004"
                     /evalue="1.10E-14"
                     /label="C2_dual_024-063"
                     /locus_tag="ctg46_1"
                     /protein_end="344"
                     /protein_start="306"
                     /score="47.0"
                     /tool="antismash"
                     /translation="YLLVSQMAFADRNLLERYLGAVQQVVDRHDILRTAFVW"
     CDS_motif       1246..1311
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0005"
                     /evalue="8.30E-06"
                     /label="C3_dual_135-156"
                     /locus_tag="ctg46_1"
                     /protein_end="437"
                     /protein_start="415"
                     /score="18.7"
                     /tool="antismash"
                     /translation="QHHLIGDHTTLEVMHAEVRAML"
     CDS_motif       1345..1386
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0006"
                     /evalue="3.50E-06"
                     /label="C4_dual_169-182"
                     /locus_tag="ctg46_1"
                     /protein_end="462"
                     /protein_start="448"
                     /score="19.5"
                     /tool="antismash"
                     /translation="PFRNLVAQARLAVD"
     CDS_motif       1624..1719
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0007"
                     /evalue="1.10E-16"
                     /label="C5_dual_260-291"
                     /locus_tag="ctg46_1"
                     /protein_end="573"
                     /protein_start="541"
                     /score="52.8"
                     /tool="antismash"
                     /translation="QVVFGTVLFGRMHAGAGADRTMGLFINTLPVR"
     CDS_motif       1771..1848
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg46_1_0008"
                     /evalue="4.20E-12"
                     /label="C67_dual_14fromHMM"
                     /locus_tag="ctg46_1"
                     /protein_end="616"
                     /protein_start="590"
                     /score="38.2"
                     /tool="antismash"
                     /translation="QLSELLAHEHASLALAQRCSGVAAPA"
ORIGIN
        1 ctgaaccgcc ccgagctgac agccgagcgg ttcatcgcca gtccctttgt ggacggcgat
       61 cggctgtaca ggaccggcga cctcgggcgt tatctgccgg acggcaatct ggagttcctg
      121 ggccgcaatg acgaccaagt gaagatccgc ggcttccgca tcgagccggg cgagatcgcg
      181 gcgcggcttt gcgaacacgc cttggtgcgc gaggcggtgg tggtggcgca acaggatcgc
      241 cgcggcgaca agcacctcgt cggctatgtg gtatgtgcgc ccgaagctca atcggatgag
      301 gtggatggcc ctgaccttgc caacaccttg cgcgcgcact tgagtagccg gctgccggac
      361 tacatggtgc cgtcggcgtt cgtgcggctt gaggtgctgc cgctgacggt gaacggcaag
      421 ctcgatcgca aggcgctgcc gccgccggct gacgatgcct atgcacgggc ggcctatgag
      481 gcgccgcagg gcgcagtcga gacggcgttg gcacggatct gggccgagct tctcgggctg
      541 gagcgggtcg gacgcaacga ccacttcttc gaactgggcg gccactcgct tctagcggtg
      601 caggtgtcga gccggctgtc gcaggctgtt ggagtcgaac tgccgctgtc gacgctgttc
      661 gccacgccgg tgctggccga ttttgccgca agccttggcc gtcctcacga agtggcggtg
      721 cctgccagcc tgatcaccga gcagagtacg gcgatcacgc cgcagatgct gccgctgatc
      781 gagctggccc agcctgagat cgaccggatc atcgccacgg tgcctggtgg cgtccgcaac
      841 atccaggaca tttatgcgct gacgccgctg caggacggca tcctgttcca tcatctgctg
      901 gccagccggg gtgatcccta tctgctggtg tcgcagatgg cgtttgccga ccgcaacctg
      961 ttggagcgct atcttggtgc ggttcagcaa gtggtggacc ggcacgacat cctgcgcacg
     1021 gcgtttgtgt gggagggact gtcgagcccg gcccaggtgg tgtggcgtcg tgcctcgctt
     1081 caggtgagcg aggtcgagct ggagggctgt gatggttgcg ccgccgatga gctccggcgc
     1141 cggtttgatc cgcgccggca gcgcatcgat cttggccggg cacccttgtt gcggtttgtg
     1201 accgcgcgcg atcccggcag cgggcgctgg ctgctgctgg agctgcagca tcatttgatc
     1261 ggggatcaca cgacgctgga agtgatgcat gccgaggtgc gggccatgct ggcagggcgg
     1321 gccgatgaac tggccgcgcc gctgccgttc cgtaatctgg tggcgcaggc gcgtcttgct
     1381 gttgatgcca aggcgcatga agagttcttc cgagggctgc tggccgacat cgacgagccg
     1441 accatgccgt tcggcttgag cgaggtctac ggcgacggca gcggcgtcgg cgaggcgcat
     1501 cggatgctgc cgcaggcgct caacgagcgg ctgcgcgaac aggcgcggcg gctcggggtg
     1561 agcctggcca gcctgtgcca tctggcctgg ggacaggtgg tggcgcgcag cagtggccgc
     1621 gagcaggtgg tgttcggcac ggtgctgttc ggccggatgc atgcgggtgc gggcgccgac
     1681 cgcacaatgg gcctgttcat caacaccctg cctgtgcggc ttgccttgga cgagaccggg
     1741 gttgcggcga gcgtgcgcag cacccacgcg cagctttccg agctgctggc gcacgagcat
     1801 gcctcgctgg cgctggcgca acgctgcagc ggcgttgcgg cgccggcgcc gctgttcagc
     1861 gcgctgttga actaccgcca taacaggccg gcagccacgc ccggctccgg accggatgat
     1921 gtcctgtcgg gcgtggaatg gctcggcgag gaggagcgca ccaactatcc cgtgaccctg
     1981 tcggtggagg attatggcga agcgctcggc ctgacggcgc aggtggcggc gcctgtctct
     2041 gcggatcgcc tctgcggcta tatgcagcgc gcgctcgagc aattggcgca ggcgctggag
     2101 catgccccca acacgccggt gcgcgagctc gacatcctgc cggcggacga acgcaattat
     2161 ctgctggagg agttgaaccg gaccgcggcg gcctatccgt cggagcggtg catccatgag
     2221 ctgttcgagg cgcaggtgca aaaggcgccg gacgcgatgg cggtggtcca tgaggacgag
     2281 cgcctgagct atggcgagct caacgcgcgg gccaaccggc tggcgcatca gctgatcgca
     2341 ctcggagtaa ggcc
//
